Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0012392

Physical Location & Seq

Genomic Coordinates Forward (+)

41978296 .. 41979639

Gene Structure 1344 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1305000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 714 bp

ATGGCGGATTCTGAAGCATCGCAGTCTACAGAAACGGTGCGAGTGGACGAGAAGAATCTGAAGTATTTGGAGTTCGTTCAAGTGGCGTCGATTTACGTGGTGGTGTGCTTCTCGAGCCTCTACGAGTACGCGAAGGAGAACTCCGGTCCGCTTAAACCGGGTGTTCAGACGGTGGAAGGCACCGTCCGAACCGTAACCGGGCCGGTCTACGAGAAGTTCCACGACCTTCCCTTCCAACTCCTCAGATTTGTCGATCGCAAGGTGGACGAGTCCTTGAGCGAGGTGGACCGTCACGTGCCGGTTCTGGTGAAGCAGGTGTCGAGCCAGGCGCTATCGGTGGCGAGAGCGGTGGAGCGAGGCGGCTTGGTGGACACGGCTAAGAATCTCACCGGGAGCTTGTACGCTAAATGCGAACCCGTGGCGGAGCAGGTGTACTACAAGTACGAGCCGGTGGCAGAGCAGTACGCCGTGTCGGCTTGGCGCGCGCTGAATCGGCTCCCGCTGTTTCCGCAGGTGGTTCATATAACGGTCCCCACCGCGTCGTACTGGTCCGACAAGTACAATCGGGCCGTCGGGTACACCGTGAACAGAGGCTACTCCGTGGCGGCGTATCTGCCGTTGATTCCGACGGAGAGAATTGCCAAGTTGTTTGATGAAGCCGAGAACGGGGTTGCCGTTTCGACGAACGGTGGGGATGTTGTAGTGGAGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005811 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0016043 GO:0019915 GO:0032502 GO:0033036 GO:0034389 GO:0040007 GO:0040008 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044444 GO:0044464 GO:0045927 GO:0048518 GO:0048583 GO:0048584 GO:0048589 GO:0050789 GO:0051179 GO:0051235 GO:0065007 GO:0065008 GO:0071840 GO:0080134 GO:0080186 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

REF

Protein Analysis

238

Amino Acids

26.32

Weight (kDa)

5.35

Isoelectric Point (pI)

27.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REF	PF05755	16 - 218	2.6e-86	Rubber elongation factor protein (REF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47780
fragaria_vesca	FvH4_2g25450
malus_domestica	MD03G1285600.v1.1 MD11G1305000.v1.1
prunus_persica	Prupe.8G258700_v2.0.a1 Prupe.8G258700_v2.0.a1
pyrus_communis	pycom11g26680
rosa_chinensis	RchiOBHm_Chr6g0293681
rosa_laevigata	RLG00000011933
rosa_multiflora	Rmu_co8092110.1_g000001 Rmu_sc0008436.1_g000002 Rmu_sc0028689.1_g000001
rosa_roxburghii	Rroxscaffold_7G00173990
rosa_rugosa	Rorug06G0237400
rosa_samantha	Rh6AG350000 Rh6BG357500 Rh6CG364300 Rh6DG351200
rosa_wichuraiana	Rw6G030470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	304, 418, 502
Acc36I	ACCTGC	3 cut(s)	304, 418, 502
AccB1I	GGYRCC	1 cut(s)	179
AccBSI	CCGCTC	1 cut(s)	347
AccI	GTMKAC	2 cut(s)	26, 207
AccII	CGCG	4 cut(s)	131, 483, 485, 539
AciI	CCGC	9 cut(s)	5, 149, 347, 360, 422, 500, 509, 537, 605
AcuI	CTGAAG	2 cut(s)	33, 80
AcvI	CACGTG	1 cut(s)	295
AcyI	GRCGYC	1 cut(s)	86
AfaI	GTAC	8 cut(s)	128, 401, 434, 443, 464, 545, 560, 578
AfiI	CCNNNNNNNGG	2 cut(s)	198, 666
AgsI	TTSAA	1 cut(s)	80
AjnI	CCWGG	1 cut(s)	324
AluBI	AGCT	1 cut(s)	396
AluI	AGCT	1 cut(s)	396
Ama87I	CYCGRG	1 cut(s)	112
AoxI	GGCC	2 cut(s)	200, 567
AspLEI	GCGC	4 cut(s)	331, 483, 485, 487
AspS9I	GGNCC	6 cut(s)	146, 200, 286, 529, 549, 567
AsuC2I	CCSGG	3 cut(s)	159, 199, 391
AsuHPI	GGTGA	2 cut(s)	319, 379
AvaI	CYCGRG	1 cut(s)	112
AvaII	GGWCC	4 cut(s)	146, 286, 529, 549
BanI	GGYRCC	1 cut(s)	179
BbrPI	CACGTG	1 cut(s)	295
BceAI	ACGGC	5 cut(s)	390, 452, 554, 601, 659
BciT130I	CCWGG	1 cut(s)	326
BcnI	CCSGG	3 cut(s)	159, 199, 391
BfmI	CTRYAG	1 cut(s)	27
BfoI	RGCGCY	1 cut(s)	332
BfuAI	ACCTGC	3 cut(s)	304, 418, 502
BglI	GCCNNNNNGGC	1 cut(s)	473
BisI	GCNGC	2 cut(s)	361, 606
BlsI	GCNGC	2 cut(s)	362, 607
Bme1390I	CCNGG	4 cut(s)	159, 199, 326, 391
Bme18I	GGWCC	4 cut(s)	146, 286, 529, 549
BmeT110I	CYCGRG	1 cut(s)	112
BmgT120I	GGNCC	6 cut(s)	146, 200, 286, 529, 549, 567
BmiI	GGNNCC	3 cut(s)	181, 497, 531
BmrFI	CCNGG	4 cut(s)	159, 199, 326, 391
BmsI	GCATC	1 cut(s)	26
BpuEI	CTTGAG	1 cut(s)	295
BpuMI	CCSGG	3 cut(s)	159, 199, 391
BsaAI	YACGTR	2 cut(s)	97, 295
BsaHI	GRCGYC	1 cut(s)	86
BsaJI	CCNNGG	2 cut(s)	417, 600
BsaWI	WCCGGW	1 cut(s)	143
BsaXI	ACNNNNNCTCC	2 cut(s)	416, 446
Bsc4I	CCNNNNNNNGG	2 cut(s)	198, 666
Bse118I	RCCGGY	3 cut(s)	202, 298, 448
Bse1I	ACTGG	1 cut(s)	551
BseBI	CCWGG	1 cut(s)	326
BseDI	CCNNGG	2 cut(s)	417, 600
BseGI	GGATG	1 cut(s)	700
BseLI	CCNNNNNNNGG	2 cut(s)	198, 666
BseMII	CTCAG	1 cut(s)	256
BseNI	ACTGG	1 cut(s)	551
BsePI	GCGCGC	2 cut(s)	481, 483
BseRI	GAGGAG	1 cut(s)	230
Bsh1236I	CGCG	4 cut(s)	131, 483, 485, 539
Bsh1285I	CGRYCG	1 cut(s)	256
BshFI	GGCC	2 cut(s)	202, 569
BshNI	GGYRCC	1 cut(s)	179
BsiEI	CGRYCG	1 cut(s)	256
BsiHKCI	CYCGRG	1 cut(s)	112
BsiSI	CCGG	7 cut(s)	144, 158, 198, 203, 299, 390, 449
BslFI	GGGAC	1 cut(s)	515
BslI	CCNNNNNNNGG	2 cut(s)	198, 666
BsmFI	GGGAC	1 cut(s)	515
BsnI	GGCC	2 cut(s)	202, 569
BsoBI	CYCGRG	1 cut(s)	112
Bsp143I	GATC	1 cut(s)	253
BspACI	CCGC	9 cut(s)	5, 149, 347, 360, 422, 500, 509, 537, 605
BspANI	GGCC	2 cut(s)	202, 569
BspCNI	CTCAG	1 cut(s)	255
BspFNI	CGCG	4 cut(s)	131, 483, 485, 539
BspLI	GGNNCC	3 cut(s)	181, 497, 531
BspMI	ACCTGC	3 cut(s)	304, 418, 502
BspT107I	GGYRCC	1 cut(s)	179
BsrBI	CCGCTC	1 cut(s)	347
BsrFI	RCCGGY	3 cut(s)	202, 298, 448
BsrI	ACTGG	1 cut(s)	551
BssAI	RCCGGY	3 cut(s)	202, 298, 448
BssECI	CCNNGG	2 cut(s)	417, 600
BssHII	GCGCGC	2 cut(s)	481, 483
BssMI	GATC	1 cut(s)	253
BssNI	GRCGYC	1 cut(s)	86
Bst2UI	CCWGG	1 cut(s)	326
Bst4CI	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 529, 583, 689
BstACI	GRCGYC	1 cut(s)	86
BstBAI	YACGTR	2 cut(s)	97, 295
BstC8I	GCNNGC	2 cut(s)	483, 485
BstDEI	CTNAG	2 cut(s)	242, 378
BstDSI	CCRYGG	2 cut(s)	417, 600
BstF5I	GGATG	1 cut(s)	700
BstFNI	CGCG	4 cut(s)	131, 483, 485, 539
BstH2I	RGCGCY	1 cut(s)	332
BstHHI	GCGC	4 cut(s)	331, 483, 485, 487
BstKTI	GATC	1 cut(s)	256
BstMBI	GATC	1 cut(s)	253
BstMCI	CGRYCG	1 cut(s)	256
BstMWI	GCNNNNNNNGC	5 cut(s)	114, 473, 482, 493, 508
BstNI	CCWGG	1 cut(s)	326
BstSCI	CCNGG	4 cut(s)	157, 197, 324, 389
BstSFI	CTRYAG	1 cut(s)	27
BstUI	CGCG	4 cut(s)	131, 483, 485, 539
BsuRI	GGCC	2 cut(s)	202, 569
BtgI	CCRYGG	2 cut(s)	417, 600
BtgZI	GCGATG	1 cut(s)	3
BtsCI	GGATG	1 cut(s)	700
BveI	ACCTGC	3 cut(s)	304, 418, 502
Cac8I	GCNNGC	2 cut(s)	483, 485
CfoI	GCGC	4 cut(s)	331, 483, 485, 487
Cfr10I	RCCGGY	3 cut(s)	202, 298, 448
Cfr13I	GGNCC	6 cut(s)	146, 200, 286, 529, 549, 567
CpoI	CGGWCCG	1 cut(s)	146
CseI	GACGC	2 cut(s)	75, 528
Csp6I	GTAC	8 cut(s)	127, 400, 433, 442, 463, 544, 559, 577
CspI	CGGWCCG	1 cut(s)	146
CviQI	GTAC	8 cut(s)	127, 400, 433, 442, 463, 544, 559, 577
DdeI	CTNAG	2 cut(s)	242, 378
DpnI	GATC	1 cut(s)	255
DpnII	GATC	1 cut(s)	253
EciI	GGCGGA	2 cut(s)	20, 437
Eco47I	GGWCC	4 cut(s)	146, 286, 529, 549
Eco57I	CTGAAG	2 cut(s)	33, 80
Eco72I	CACGTG	1 cut(s)	295
Eco88I	CYCGRG	1 cut(s)	112
EcoRII	CCWGG	1 cut(s)	324
FaiI	YATR	2 cut(s)	522, 524
FaqI	GGGAC	1 cut(s)	515
FauI	CCCGC	1 cut(s)	507
FblI	GTMKAC	2 cut(s)	26, 207
Fnu4HI	GCNGC	2 cut(s)	361, 606
FokI	GGATG	1 cut(s)	707
Fsp4HI	GCNGC	2 cut(s)	361, 606
GlaI	GCGC	4 cut(s)	330, 482, 484, 486
GluI	GCNGC	2 cut(s)	361, 606
HaeII	RGCGCY	1 cut(s)	332
HaeIII	GGCC	2 cut(s)	202, 569
HapII	CCGG	7 cut(s)	144, 158, 198, 203, 299, 390, 449
HgaI	GACGC	2 cut(s)	75, 528
HhaI	GCGC	4 cut(s)	331, 483, 485, 487
Hin1I	GRCGYC	1 cut(s)	86
Hin6I	GCGC	4 cut(s)	329, 481, 483, 485
HinP1I	GCGC	4 cut(s)	329, 481, 483, 485
HinfI	GANTC	6 cut(s)	8, 55, 269, 382, 490, 622
HpaII	CCGG	7 cut(s)	144, 158, 198, 203, 299, 390, 449
HphI	GGTGA	2 cut(s)	319, 379
Hpy166II	GTNNAC	9 cut(s)	27, 46, 208, 265, 286, 370, 433, 579, 586
Hpy188I	TCNGA	7 cut(s)	13, 60, 168, 188, 245, 553, 627
Hpy188III	TCNNGA	1 cut(s)	112
Hpy8I	GTNNAC	9 cut(s)	27, 46, 208, 265, 286, 370, 433, 579, 586
Hpy99I	CGWCG	5 cut(s)	91, 544, 575, 631, 685
HpyAV	CCTTC	4 cut(s)	127, 170, 236, 241
HpyCH4III	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 529, 583, 689
HpyCH4IV	ACGT	2 cut(s)	96, 294
HpyF10VI	GCNNNNNNNGC	5 cut(s)	114, 473, 482, 493, 508
HpyF3I	CTNAG	2 cut(s)	242, 378
HpySE526I	ACGT	2 cut(s)	96, 294
Hsp92I	GRCGYC	1 cut(s)	86
HspAI	GCGC	4 cut(s)	329, 481, 483, 485
Kzo9I	GATC	1 cut(s)	253
LmnI	GCTCC	5 cut(s)	352, 393, 424, 501, 706
LweI	GCATC	1 cut(s)	26
MaeII	ACGT	2 cut(s)	96, 294
MaeIII	GTNAC	2 cut(s)	193, 290
MalI	GATC	1 cut(s)	255
MbiI	CCGCTC	1 cut(s)	347
MboI	GATC	1 cut(s)	253
MboII	GAAGA	1 cut(s)	64
MluCI	AATT	1 cut(s)	636
MlyI	GAGTC	1 cut(s)	278
MmeI	TCCRAC	3 cut(s)	259, 576, 650
MnlI	CCTC	5 cut(s)	128, 251, 274, 350, 584
MseI	TTAA	1 cut(s)	153
MspA1I	CMGCKG	1 cut(s)	502
MspI	CCGG	7 cut(s)	144, 158, 198, 203, 299, 390, 449
MspR9I	CCNGG	4 cut(s)	159, 199, 326, 391
MvaI	CCWGG	1 cut(s)	326
MvnI	CGCG	4 cut(s)	131, 483, 485, 539
MwoI	GCNNNNNNNGC	5 cut(s)	114, 473, 482, 493, 508
NciI	CCSGG	3 cut(s)	159, 199, 391
NdeII	GATC	1 cut(s)	253
NlaIV	GGNNCC	3 cut(s)	181, 497, 531
NmeAIII	GCCGAG	1 cut(s)	685
NmuCI	GTSAC	1 cut(s)	290
PaeR7I	CTCGAG	1 cut(s)	112
PaqCI	CACCTGC	3 cut(s)	304, 418, 502
PauI	GCGCGC	2 cut(s)	481, 483
PcsI	WCGNNNNNNNCGW	3 cut(s)	408, 579, 672
PfeI	GAWTC	5 cut(s)	8, 55, 382, 490, 622
PkrI	GCNGC	2 cut(s)	362, 607
Ple19I	CGATCG	1 cut(s)	256
PleI	GAGTC	1 cut(s)	277
PmaCI	CACGTG	1 cut(s)	295
PmlI	CACGTG	1 cut(s)	295
PpsI	GAGTC	1 cut(s)	277
Ppu21I	YACGTR	2 cut(s)	97, 295
Psp6I	CCWGG	1 cut(s)	324
PspCI	CACGTG	1 cut(s)	295
PspGI	CCWGG	1 cut(s)	324
PspN4I	GGNNCC	3 cut(s)	181, 497, 531
PspPI	GGNCC	6 cut(s)	146, 200, 286, 529, 549, 567
PsrI	GAACNNNNNNTAC	4 cut(s)	56, 88, 578, 610
PteI	GCGCGC	2 cut(s)	481, 483
PvuI	CGATCG	1 cut(s)	256
RsaI	GTAC	8 cut(s)	128, 401, 434, 443, 464, 545, 560, 578
RsaNI	GTAC	8 cut(s)	127, 400, 433, 442, 463, 544, 559, 577
Rsr2I	CGGWCCG	1 cut(s)	146
RsrII	CGGWCCG	1 cut(s)	146
SaqAI	TTAA	1 cut(s)	153
SatI	GCNGC	2 cut(s)	361, 606
Sau3AI	GATC	1 cut(s)	253
Sau96I	GGNCC	6 cut(s)	146, 200, 286, 529, 549, 567
SchI	GAGTC	1 cut(s)	278
ScrFI	CCNGG	4 cut(s)	159, 199, 326, 391
SetI	ASST	9 cut(s)	99, 228, 264, 285, 297, 318, 398, 432, 516
SfaNI	GCATC	1 cut(s)	26
SfcI	CTRYAG	1 cut(s)	27
Sfr274I	CTCGAG	1 cut(s)	112
SinI	GGWCC	4 cut(s)	146, 286, 529, 549
SlaI	CTCGAG	1 cut(s)	112
SmlI	CTYRAG	2 cut(s)	112, 274
SmoI	CTYRAG	2 cut(s)	112, 274
Sse9I	AATT	1 cut(s)	636
SsiI	CCGC	9 cut(s)	5, 149, 347, 360, 422, 500, 509, 537, 605
StyD4I	CCNGG	4 cut(s)	157, 197, 324, 389
TaaI	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 529, 583, 689
TaiI	ACGT	2 cut(s)	99, 297
TaqI	TCGA	5 cut(s)	89, 113, 252, 320, 680
TasI	AATT	1 cut(s)	636
TatI	WGTACW	2 cut(s)	432, 558
TauI	GCSGC	2 cut(s)	363, 608
TfiI	GAWTC	5 cut(s)	8, 55, 382, 490, 622
Tru1I	TTAA	1 cut(s)	153
Tru9I	TTAA	1 cut(s)	153
TseFI	GTSAC	1 cut(s)	290
Tsp45I	GTSAC	1 cut(s)	290
TspDTI	ATGAA	2 cut(s)	509, 669
TspGWI	ACGGA	2 cut(s)	589, 644
VpaK11BI	GGWCC	4 cut(s)	146, 286, 529, 549
XhoI	CTCGAG	1 cut(s)	112
XmiI	GTMKAC	2 cut(s)	26, 207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.