FvH4_2g25450

Rubber elongation factor protein (REF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012392
Physical Location & Seq
Forward (+)
20641669 .. 20644137
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25450.t1

Sequence Viewer

Length: 717 bp
ATGGCGGATGCCGAATCTTCACGCCCAATCAATACGGTGGATTGCGATGAGAAGAAGCTGAAGTATCTGGAATTTGTGGAGGTGGCGGCGATTTACGCCGTGCTCTGCTTCTCGAGCCTCTACGAGTACGCCAAGGAAAACGCCGGTCCTCTGAAACCGGGGGTTCAGACGGTGGAAGGCACCGTCAAAACGGTGATCGGACCGGTCTACGAGAAGTTCCAGGACCTTCCTTTCCAGCTCCTTAAATTGGTGGATCGCAAGGTGGACGAGTCGCTGAGCGAGTTGGACAGTCACGTGCCGCGTCTGGTTAAGACGGCGTCAAACAAGGCTCTGTCTGTGGCGACGGAGGTTCAGCGGGCCGGGTTGGTGGACGCGGCGCGTAGCATCACGGCGAGCGCGTACTCGAAATACGAGCCGATGGCGGAGGAGGTGTACTACAAGTACGAACCGGTGGCGGAGCAGTACGCGGTGTCCGCTTGGCGGTCGCTGAACCGGATGCCGCTGGTTCCTCAGGTGGCTCAGATTATGGTTCCCACGGCGGCCTATTGGTCAGAGAAGTACAATCAGGCTGTTTGTTACACGGCGGAGAAGGGGTTCCCCGTGGCGGGTCACCTGCCGTTGATTCCGACGGAGAGGATTGCGAAGGCGTTTGAGGAAGCCGAGCATGTTTCTCCTGACGTTTCGACGAATGCCGGAGCCATCGGCCTGGAACAGTGA

Protein Analysis

239

Amino Acids

26.27

Weight (kDa)

5.06

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 16 - 218 5.1e-87 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47780
fragaria_vesca FvH4_2g25450
malus_domestica MD03G1285600.v1.1 MD11G1305000.v1.1
prunus_persica Prupe.8G258700_v2.0.a1 Prupe.8G258700_v2.0.a1
pyrus_communis pycom11g26680
rosa_chinensis RchiOBHm_Chr6g0293681
rosa_laevigata RLG00000011933
rosa_multiflora Rmu_co8092110.1_g000001 Rmu_sc0008436.1_g000002 Rmu_sc0028689.1_g000001
rosa_roxburghii Rroxscaffold_7G00173990
rosa_rugosa Rorug06G0237400
rosa_samantha Rh6AG350000 Rh6BG357500 Rh6CG364300 Rh6DG351200
rosa_wichuraiana Rw6G030470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 207
AccII CGCG 5 cut(s) 301, 374, 379, 398, 467
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 80
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 6 cut(s) 128, 401, 434, 443, 464, 560
AfiI CCNNNNNNNGG 4 cut(s) 247, 480, 604, 605
AgeI ACCGGT 2 cut(s) 202, 448
AjnI CCWGG 2 cut(s) 219, 705
AloI GAACNNNNNNTCC 2 cut(s) 578, 610
AluBI AGCT 2 cut(s) 58, 238
AluI AGCT 2 cut(s) 58, 238
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 3 cut(s) 357, 540, 703
ApoI RAATTY 1 cut(s) 71
AsiGI ACCGGT 2 cut(s) 202, 448
Asp700I GAANNNNTTC 1 cut(s) 593
AspLEI GCGC 2 cut(s) 379, 398
AspS9I GGNCC 4 cut(s) 146, 200, 223, 357
AsuC2I CCSGG 2 cut(s) 159, 361
AsuHPI GGTGA 2 cut(s) 205, 602
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 3 cut(s) 146, 200, 223
AxyI CCTNAGG 1 cut(s) 510
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 105
BccI CCATC 2 cut(s) 412, 707
BceAI ACGGC 6 cut(s) 83, 330, 405, 552, 597, 601
BciT130I CCWGG 2 cut(s) 221, 707
BcnI CCSGG 2 cut(s) 159, 361
BfuAI ACCTGC 1 cut(s) 621
BisI GCNGC 5 cut(s) 87, 299, 375, 500, 540
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 5 cut(s) 88, 300, 376, 501, 541
Bme1390I CCNGG 4 cut(s) 159, 221, 361, 707
Bme18I GGWCC 3 cut(s) 146, 200, 223
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 146, 200, 223, 357
BmiI GGNNCC 5 cut(s) 181, 507, 531, 596, 697
BmrFI CCNGG 4 cut(s) 159, 221, 361, 707
BmsI GCATC 2 cut(s) 393, 486
Bpu1102I GCTNAGC 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 159, 361
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 132, 158, 534, 600
BsaWI WCCGGW 3 cut(s) 202, 448, 492
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 578, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 480, 604, 605
Bse118I RCCGGY 3 cut(s) 143, 202, 448
Bse21I CCTNAGG 1 cut(s) 510
BseBI CCWGG 2 cut(s) 221, 707
BseDI CCNNGG 4 cut(s) 132, 158, 534, 600
BseGI GGATG 2 cut(s) 13, 501
BseLI CCNNNNNNNGG 4 cut(s) 247, 480, 604, 605
BseMII CTCAG 3 cut(s) 266, 524, 533
BseRI GAGGAG 1 cut(s) 440
Bsh1236I CGCG 5 cut(s) 301, 374, 379, 398, 467
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 3 cut(s) 359, 542, 705
BshNI GGYRCC 1 cut(s) 179
BshTI ACCGGT 2 cut(s) 202, 448
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 7 cut(s) 144, 158, 203, 360, 449, 493, 693
BslI CCNNNNNNNGG 4 cut(s) 247, 480, 604, 605
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 3 cut(s) 359, 542, 705
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 195, 253
Bsp1720I GCTNAGC 1 cut(s) 275
BspANI GGCC 3 cut(s) 359, 542, 705
BspCNI CTCAG 3 cut(s) 267, 523, 532
BspFNI CGCG 5 cut(s) 301, 374, 379, 398, 467
BspLI GGNNCC 5 cut(s) 181, 507, 531, 596, 697
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 179
BsrFI RCCGGY 3 cut(s) 143, 202, 448
BssAI RCCGGY 3 cut(s) 143, 202, 448
BssECI CCNNGG 4 cut(s) 132, 158, 534, 600
BssMI GATC 2 cut(s) 195, 253
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 2 cut(s) 221, 707
Bst4CI ACNGT 6 cut(s) 37, 172, 184, 193, 290, 714
BstACI GRCGYC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 2 cut(s) 357, 394
BstDEI CTNAG 3 cut(s) 275, 510, 519
BstDSI CCRYGG 2 cut(s) 534, 600
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 2 cut(s) 13, 501
BstFNI CGCG 5 cut(s) 301, 374, 379, 398, 467
BstHHI GCGC 2 cut(s) 379, 398
BstKTI GATC 2 cut(s) 198, 256
BstMBI GATC 2 cut(s) 195, 253
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 3 cut(s) 95, 114, 473
BstNI CCWGG 2 cut(s) 221, 707
BstNSI RCATGY 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 4 cut(s) 157, 219, 359, 705
BstUI CGCG 5 cut(s) 301, 374, 379, 398, 467
BstXI CCANNNNNNTGG 1 cut(s) 706
Bsu36I CCTNAGG 1 cut(s) 510
BsuRI GGCC 3 cut(s) 359, 542, 705
BtgI CCRYGG 2 cut(s) 534, 600
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 13, 501
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 2 cut(s) 357, 394
CfoI GCGC 2 cut(s) 379, 398
Cfr10I RCCGGY 3 cut(s) 143, 202, 448
Cfr13I GGNCC 4 cut(s) 146, 200, 223, 357
CpoI CGGWCCG 1 cut(s) 200
CseI GACGC 3 cut(s) 290, 306, 380
Csp6I GTAC 6 cut(s) 127, 400, 433, 442, 463, 559
CspAI ACCGGT 2 cut(s) 202, 448
CspI CGGWCCG 1 cut(s) 200
CviAII CATG 1 cut(s) 665
CviQI GTAC 6 cut(s) 127, 400, 433, 442, 463, 559
DdeI CTNAG 3 cut(s) 275, 510, 519
DpnI GATC 2 cut(s) 197, 255
DpnII GATC 2 cut(s) 195, 253
EciI GGCGGA 4 cut(s) 20, 437, 470, 599
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 3 cut(s) 146, 200, 223
Eco57I CTGAAG 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 295
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 219, 705
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 1 cut(s) 668
FaiI YATR 2 cut(s) 527, 666
FatI CATG 1 cut(s) 664
FauI CCCGC 2 cut(s) 348, 598
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 5 cut(s) 87, 299, 375, 500, 540
FokI GGATG 2 cut(s) 20, 508
Fsp4HI GCNGC 5 cut(s) 87, 299, 375, 500, 540
GlaI GCGC 2 cut(s) 378, 397
GluI GCNGC 5 cut(s) 87, 299, 375, 500, 540
HaeIII GGCC 3 cut(s) 359, 542, 705
HapII CCGG 7 cut(s) 144, 158, 203, 360, 449, 493, 693
HgaI GACGC 3 cut(s) 290, 306, 380
HhaI GCGC 2 cut(s) 379, 398
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 1 cut(s) 668
Hin6I GCGC 2 cut(s) 377, 396
HinP1I GCGC 2 cut(s) 377, 396
HinfI GANTC 3 cut(s) 14, 269, 622
HpaII CCGG 7 cut(s) 144, 158, 203, 360, 449, 493, 693
HphI GGTGA 2 cut(s) 205, 602
Hpy166II GTNNAC 4 cut(s) 208, 265, 370, 433
Hpy188I TCNGA 6 cut(s) 153, 168, 200, 522, 553, 627
Hpy188III TCNNGA 3 cut(s) 68, 112, 674
Hpy8I GTNNAC 4 cut(s) 208, 265, 370, 433
Hpy99I CGWCG 3 cut(s) 346, 631, 688
HpyAV CCTTC 4 cut(s) 170, 236, 583, 637
HpyCH4III ACNGT 6 cut(s) 37, 172, 184, 193, 290, 714
HpyCH4IV ACGT 2 cut(s) 294, 678
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 114, 473
HpyF3I CTNAG 3 cut(s) 275, 510, 519
HpySE526I ACGT 2 cut(s) 294, 678
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 1 cut(s) 668
HspAI GCGC 2 cut(s) 377, 396
Kzo9I GATC 2 cut(s) 195, 253
LmnI GCTCC 3 cut(s) 243, 457, 695
LweI GCATC 2 cut(s) 393, 486
MaeII ACGT 2 cut(s) 294, 678
MaeIII GTNAC 3 cut(s) 290, 575, 608
MalI GATC 2 cut(s) 197, 255
MboI GATC 2 cut(s) 195, 253
MboII GAAGA 2 cut(s) 9, 64
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 71, 245
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 2 cut(s) 264, 650
MnlI CCTC 9 cut(s) 73, 128, 159, 340, 418, 421, 519, 627, 646
MroXI GAANNNNTTC 1 cut(s) 593
MseI TTAA 2 cut(s) 243, 309
MspA1I CMGCKG 2 cut(s) 355, 502
MspI CCGG 7 cut(s) 144, 158, 203, 360, 449, 493, 693
MspR9I CCNGG 4 cut(s) 159, 221, 361, 707
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 2 cut(s) 221, 707
MvnI CGCG 5 cut(s) 301, 374, 379, 398, 467
MwoI GCNNNNNNNGC 3 cut(s) 95, 114, 473
NciI CCSGG 2 cut(s) 159, 361
NdeII GATC 2 cut(s) 195, 253
NlaIII CATG 1 cut(s) 668
NlaIV GGNNCC 5 cut(s) 181, 507, 531, 596, 697
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 2 cut(s) 290, 608
NspI RCATGY 1 cut(s) 668
PaeR7I CTCGAG 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 14, 622
PflFI GACNNNGTC 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 202, 448
PkrI GCNGC 5 cut(s) 88, 300, 376, 501, 541
PleI GAGTC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 219, 705
PspCI CACGTG 1 cut(s) 295
PspEI GGTNACC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 219, 705
PspN4I GGNNCC 5 cut(s) 181, 507, 531, 596, 697
PspPI GGNCC 4 cut(s) 146, 200, 223, 357
PspPPI RGGWCCY 1 cut(s) 223
PsyI GACNNNGTC 1 cut(s) 316
RsaI GTAC 6 cut(s) 128, 401, 434, 443, 464, 560
RsaNI GTAC 6 cut(s) 127, 400, 433, 442, 463, 559
Rsr2I CGGWCCG 1 cut(s) 200
RsrII CGGWCCG 1 cut(s) 200
SaqAI TTAA 2 cut(s) 243, 309
SatI GCNGC 5 cut(s) 87, 299, 375, 500, 540
Sau3AI GATC 2 cut(s) 195, 253
Sau96I GGNCC 4 cut(s) 146, 200, 223, 357
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 4 cut(s) 159, 221, 361, 707
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 2 cut(s) 393, 486
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 3 cut(s) 146, 200, 223
SlaI CTCGAG 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 2 cut(s) 71, 245
StyD4I CCNGG 4 cut(s) 157, 219, 359, 705
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 6 cut(s) 37, 172, 184, 193, 290, 714
TaiI ACGT 2 cut(s) 297, 681
TaqI TCGA 3 cut(s) 113, 404, 683
TasI AATT 2 cut(s) 71, 245
TatI WGTACW 2 cut(s) 432, 558
TauI GCSGC 5 cut(s) 89, 301, 377, 502, 542
TfiI GAWTC 2 cut(s) 14, 622
Tru1I TTAA 2 cut(s) 243, 309
Tru9I TTAA 2 cut(s) 243, 309
TseFI GTSAC 2 cut(s) 290, 608
Tsp45I GTSAC 2 cut(s) 290, 608
TspGWI ACGGA 2 cut(s) 359, 644
Tth111I GACNNNGTC 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 146, 200, 223
XapI RAATTY 1 cut(s) 71
XceI RCATGY 1 cut(s) 668
XhoI CTCGAG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.