Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012392

Physical Location & Seq

Genomic Coordinates Forward (+)

36517667 .. 36525628

Gene Structure 7962 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1285600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 678 bp

ATGGCAGATTCTGAAGCATCGCAGTCTACAGAAACGGTGCGAGTGGACGCGAAGAAGCTCAAGTATTTGGAATTCGTTCAGGTGGCGGCGATTTACGTGGTGGTGTGCTTCTCGAGCCTGTACGAGTACGCAAAGGAGAACTCCGGTCCGCTTAAACCAGGGGTTCAGACGGTGGAAGGCACCGTCCGAACCGTAATCGGACCGGTCTACGAGAAGTTACACGGCCTTCCCTTCCAATTCCTCAAATTCGTCGATCGCAAGGTGGATGAGTCATTGAGCGAGGTGGACCGTCACGTGCCAGTTCTGGTGAAGCAGGCGTCGAGCCAGGCGCTATCGGTGGCGAGAGAGGTGGAGCAAGGCGGCTTGGTGAGCACGGCTAAGAATATTACTGTGAGCGTGTACTACAAGTACGAGCCGGTGGCGGAGCAGTACGCCGTGTCGGCTTGGCGAGCGCTAAACCGGCTACCGCTGTTTCCATTGGTGGCTCAGATAATAGTCCCAACCGTGTCGTACTGGTCCGGCAAGTACAATCGGGCCGTCGGGTACACTGCCAACAGAGGCTACTCCGTGGCGGCGTATCTGCCGTTGATTCCGACGGAGAGGATTGCCAAGGTGTTTGAAGCGGAGAACGTGGTTGCCGTTCCGACGAACGGTGGCTCTGTTGCAGTGGAGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005811 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0016043 GO:0019915 GO:0032502 GO:0033036 GO:0034389 GO:0040007 GO:0040008 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044444 GO:0044464 GO:0045927 GO:0048518 GO:0048583 GO:0048584 GO:0048589 GO:0050789 GO:0051179 GO:0051235 GO:0065007 GO:0065008 GO:0071840 GO:0080134 GO:0080186 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

REF

Protein Analysis

226

Amino Acids

24.74

Weight (kDa)

7.74

Isoelectric Point (pI)

26.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REF	PF05755	16 - 207	2.8e-85	Rubber elongation factor protein (REF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47780
fragaria_vesca	FvH4_2g25450
malus_domestica	MD03G1285600.v1.1 MD11G1305000.v1.1
prunus_persica	Prupe.8G258700_v2.0.a1 Prupe.8G258700_v2.0.a1
pyrus_communis	pycom11g26680
rosa_chinensis	RchiOBHm_Chr6g0293681
rosa_laevigata	RLG00000011933
rosa_multiflora	Rmu_co8092110.1_g000001 Rmu_sc0008436.1_g000002 Rmu_sc0028689.1_g000001
rosa_roxburghii	Rroxscaffold_7G00173990
rosa_rugosa	Rorug06G0237400
rosa_samantha	Rh6AG350000 Rh6BG357500 Rh6CG364300 Rh6DG351200
rosa_wichuraiana	Rw6G030470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	179
AccI	GTMKAC	2 cut(s)	26, 207
AccII	CGCG	1 cut(s)	50
AciI	CCGC	7 cut(s)	86, 149, 360, 422, 467, 572, 623
AcsI	RAATTY	2 cut(s)	71, 245
AcuI	CTGAAG	1 cut(s)	33
AcvI	CACGTG	1 cut(s)	295
AcyI	GRCGYC	1 cut(s)	317
AfaI	GTAC	8 cut(s)	122, 128, 401, 410, 431, 512, 527, 545
AfeI	AGCGCT	1 cut(s)	453
AfiI	CCNNNNNNNGG	1 cut(s)	650
AgeI	ACCGGT	1 cut(s)	202
AgsI	TTSAA	1 cut(s)	620
AjnI	CCWGG	2 cut(s)	157, 324
AluBI	AGCT	1 cut(s)	58
AluI	AGCT	1 cut(s)	58
Alw21I	GWGCWC	1 cut(s)	374
AlwNI	CAGNNNCTG	1 cut(s)	11
Ama87I	CYCGRG	1 cut(s)	112
Aor51HI	AGCGCT	1 cut(s)	453
AoxI	GGCC	2 cut(s)	223, 534
ApoI	RAATTY	2 cut(s)	71, 245
AsiGI	ACCGGT	1 cut(s)	202
Asp700I	GAANNNNTTC	1 cut(s)	75
AspLEI	GCGC	2 cut(s)	331, 454
AspS9I	GGNCC	5 cut(s)	146, 200, 286, 516, 534
AsuHPI	GGTGA	2 cut(s)	319, 379
AvaI	CYCGRG	1 cut(s)	112
AvaII	GGWCC	4 cut(s)	146, 200, 286, 516
BanI	GGYRCC	1 cut(s)	179
BbrPI	CACGTG	1 cut(s)	295
Bbv12I	GWGCWC	1 cut(s)	374
BceAI	ACGGC	6 cut(s)	238, 390, 419, 521, 568, 623
BciT130I	CCWGG	2 cut(s)	159, 326
BfmI	CTRYAG	1 cut(s)	27
BfoI	RGCGCY	2 cut(s)	332, 455
BglI	GCCNNNNNGGC	1 cut(s)	440
BisI	GCNGC	3 cut(s)	87, 361, 573
BlsI	GCNGC	3 cut(s)	88, 362, 574
Bme1390I	CCNGG	2 cut(s)	159, 326
Bme18I	GGWCC	4 cut(s)	146, 200, 286, 516
BmeT110I	CYCGRG	1 cut(s)	112
BmgT120I	GGNCC	5 cut(s)	146, 200, 286, 516, 534
BmiI	GGNNCC	1 cut(s)	181
BmrFI	CCNGG	2 cut(s)	159, 326
BmsI	GCATC	1 cut(s)	26
BpuEI	CTTGAG	1 cut(s)	44
BsaAI	YACGTR	2 cut(s)	97, 295
BsaHI	GRCGYC	1 cut(s)	317
BsaJI	CCNNGG	3 cut(s)	158, 567, 609
BsaWI	WCCGGW	2 cut(s)	143, 202
Bsc4I	CCNNNNNNNGG	1 cut(s)	650
Bse118I	RCCGGY	3 cut(s)	202, 415, 459
Bse1I	ACTGG	2 cut(s)	299, 518
BseBI	CCWGG	2 cut(s)	159, 326
BseDI	CCNNGG	3 cut(s)	158, 567, 609
BseGI	GGATG	1 cut(s)	271
BseLI	CCNNNNNNNGG	1 cut(s)	650
BseMII	CTCAG	1 cut(s)	500
BseNI	ACTGG	2 cut(s)	299, 518
Bsh1236I	CGCG	1 cut(s)	50
Bsh1285I	CGRYCG	1 cut(s)	256
BshFI	GGCC	2 cut(s)	225, 536
BshNI	GGYRCC	1 cut(s)	179
BshTI	ACCGGT	1 cut(s)	202
BsiEI	CGRYCG	1 cut(s)	256
BsiHKAI	GWGCWC	1 cut(s)	374
BsiHKCI	CYCGRG	1 cut(s)	112
BsiSI	CCGG	5 cut(s)	144, 203, 416, 460, 519
BslFI	GGGAC	1 cut(s)	482
BslI	CCNNNNNNNGG	1 cut(s)	650
BsmFI	GGGAC	1 cut(s)	482
BsnI	GGCC	2 cut(s)	225, 536
BsoBI	CYCGRG	1 cut(s)	112
Bsp1286I	GDGCHC	1 cut(s)	374
Bsp143I	GATC	1 cut(s)	253
BspACI	CCGC	7 cut(s)	86, 149, 360, 422, 467, 572, 623
BspANI	GGCC	2 cut(s)	225, 536
BspCNI	CTCAG	1 cut(s)	499
BspFNI	CGCG	1 cut(s)	50
BspLI	GGNNCC	1 cut(s)	181
BspT107I	GGYRCC	1 cut(s)	179
BsrFI	RCCGGY	3 cut(s)	202, 415, 459
BsrI	ACTGG	2 cut(s)	299, 518
BssAI	RCCGGY	3 cut(s)	202, 415, 459
BssECI	CCNNGG	3 cut(s)	158, 567, 609
BssMI	GATC	1 cut(s)	253
BssNI	GRCGYC	1 cut(s)	317
BssT1I	CCWWGG	1 cut(s)	609
Bst2UI	CCWGG	2 cut(s)	159, 326
Bst4CI	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 391, 505, 653
BstACI	GRCGYC	1 cut(s)	317
BstBAI	YACGTR	2 cut(s)	97, 295
BstC8I	GCNNGC	2 cut(s)	315, 450
BstDEI	CTNAG	2 cut(s)	378, 486
BstDSI	CCRYGG	1 cut(s)	567
BstF5I	GGATG	1 cut(s)	271
BstFNI	CGCG	1 cut(s)	50
BstH2I	RGCGCY	2 cut(s)	332, 455
BstHHI	GCGC	2 cut(s)	331, 454
BstKTI	GATC	1 cut(s)	256
BstMBI	GATC	1 cut(s)	253
BstMCI	CGRYCG	1 cut(s)	256
BstMWI	GCNNNNNNNGC	5 cut(s)	114, 369, 440, 449, 460
BstNI	CCWGG	2 cut(s)	159, 326
BstSCI	CCNGG	2 cut(s)	157, 324
BstSFI	CTRYAG	1 cut(s)	27
BstUI	CGCG	1 cut(s)	50
BsuRI	GGCC	2 cut(s)	225, 536
BtgI	CCRYGG	1 cut(s)	567
BtgZI	GCGATG	1 cut(s)	3
BtsCI	GGATG	1 cut(s)	271
BtsI	GCAGTG	2 cut(s)	546, 672
BtsIMutI	CAGTG	2 cut(s)	546, 672
Cac8I	GCNNGC	2 cut(s)	315, 450
CaiI	CAGNNNCTG	1 cut(s)	11
CfoI	GCGC	2 cut(s)	331, 454
Cfr10I	RCCGGY	3 cut(s)	202, 415, 459
Cfr13I	GGNCC	5 cut(s)	146, 200, 286, 516, 534
CpoI	CGGWCCG	2 cut(s)	146, 200
CseI	GACGC	2 cut(s)	56, 306
Csp6I	GTAC	8 cut(s)	121, 127, 400, 409, 430, 511, 526, 544
CspAI	ACCGGT	1 cut(s)	202
CspI	CGGWCCG	2 cut(s)	146, 200
CviQI	GTAC	8 cut(s)	121, 127, 400, 409, 430, 511, 526, 544
DdeI	CTNAG	2 cut(s)	378, 486
DpnI	GATC	1 cut(s)	255
DpnII	GATC	1 cut(s)	253
EciI	GGCGGA	1 cut(s)	437
Eco130I	CCWWGG	1 cut(s)	609
Eco47I	GGWCC	4 cut(s)	146, 200, 286, 516
Eco47III	AGCGCT	1 cut(s)	453
Eco57I	CTGAAG	1 cut(s)	33
Eco72I	CACGTG	1 cut(s)	295
Eco88I	CYCGRG	1 cut(s)	112
EcoRI	GAATTC	1 cut(s)	71
EcoRII	CCWGG	2 cut(s)	157, 324
EcoT14I	CCWWGG	1 cut(s)	609
ErhI	CCWWGG	1 cut(s)	609
FaqI	GGGAC	1 cut(s)	482
FblI	GTMKAC	2 cut(s)	26, 207
Fnu4HI	GCNGC	3 cut(s)	87, 361, 573
FokI	GGATG	1 cut(s)	278
Fsp4HI	GCNGC	3 cut(s)	87, 361, 573
GlaI	GCGC	2 cut(s)	330, 453
GluI	GCNGC	3 cut(s)	87, 361, 573
HaeII	RGCGCY	2 cut(s)	332, 455
HaeIII	GGCC	2 cut(s)	225, 536
HapII	CCGG	5 cut(s)	144, 203, 416, 460, 519
HgaI	GACGC	2 cut(s)	56, 306
HhaI	GCGC	2 cut(s)	331, 454
Hin1I	GRCGYC	1 cut(s)	317
Hin6I	GCGC	2 cut(s)	329, 452
HinP1I	GCGC	2 cut(s)	329, 452
HinfI	GANTC	3 cut(s)	8, 269, 589
HpaII	CCGG	5 cut(s)	144, 203, 416, 460, 519
HphI	GGTGA	2 cut(s)	319, 379
Hpy166II	GTNNAC	6 cut(s)	27, 46, 208, 286, 400, 546
Hpy188I	TCNGA	7 cut(s)	13, 168, 188, 200, 489, 594, 645
Hpy188III	TCNNGA	1 cut(s)	112
Hpy8I	GTNNAC	6 cut(s)	27, 46, 208, 286, 400, 546
Hpy99I	CGWCG	5 cut(s)	254, 322, 542, 598, 649
HpyAV	CCTTC	3 cut(s)	170, 236, 241
HpyCH4III	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 391, 505, 653
HpyCH4IV	ACGT	3 cut(s)	96, 294, 630
HpyCH4V	TGCA	1 cut(s)	665
HpyF10VI	GCNNNNNNNGC	5 cut(s)	114, 369, 440, 449, 460
HpyF3I	CTNAG	2 cut(s)	378, 486
HpySE526I	ACGT	3 cut(s)	96, 294, 630
Hsp92I	GRCGYC	1 cut(s)	317
HspAI	GCGC	2 cut(s)	329, 452
Kzo9I	GATC	1 cut(s)	253
LmnI	GCTCC	3 cut(s)	352, 424, 670
LweI	GCATC	1 cut(s)	26
MaeII	ACGT	3 cut(s)	96, 294, 630
MaeIII	GTNAC	2 cut(s)	216, 290
MalI	GATC	1 cut(s)	255
MboI	GATC	1 cut(s)	253
MboII	GAAGA	1 cut(s)	64
MhlI	GDGCHC	1 cut(s)	374
MluCI	AATT	3 cut(s)	71, 236, 245
MlyI	GAGTC	1 cut(s)	278
MmeI	TCCRAC	2 cut(s)	617, 668
MnlI	CCTC	5 cut(s)	251, 274, 340, 551, 594
MroXI	GAANNNNTTC	1 cut(s)	75
MseI	TTAA	1 cut(s)	153
MspA1I	CMGCKG	1 cut(s)	469
MspI	CCGG	5 cut(s)	144, 203, 416, 460, 519
MspR9I	CCNGG	2 cut(s)	159, 326
MvaI	CCWGG	2 cut(s)	159, 326
MvnI	CGCG	1 cut(s)	50
MwoI	GCNNNNNNNGC	5 cut(s)	114, 369, 440, 449, 460
NdeII	GATC	1 cut(s)	253
NlaIV	GGNNCC	1 cut(s)	181
NmuCI	GTSAC	1 cut(s)	290
PaeR7I	CTCGAG	1 cut(s)	112
PcsI	WCGNNNNNNNCGW	1 cut(s)	636
PdmI	GAANNNNTTC	1 cut(s)	75
PfeI	GAWTC	2 cut(s)	8, 589
PinAI	ACCGGT	1 cut(s)	202
PkrI	GCNGC	3 cut(s)	88, 362, 574
Ple19I	CGATCG	1 cut(s)	256
PleI	GAGTC	1 cut(s)	277
PmaCI	CACGTG	1 cut(s)	295
PmlI	CACGTG	1 cut(s)	295
PpsI	GAGTC	1 cut(s)	277
Ppu21I	YACGTR	2 cut(s)	97, 295
Psp6I	CCWGG	2 cut(s)	157, 324
PspCI	CACGTG	1 cut(s)	295
PspGI	CCWGG	2 cut(s)	157, 324
PspN4I	GGNNCC	1 cut(s)	181
PspPI	GGNCC	5 cut(s)	146, 200, 286, 516, 534
PstNI	CAGNNNCTG	1 cut(s)	11
PvuI	CGATCG	1 cut(s)	256
RsaI	GTAC	8 cut(s)	122, 128, 401, 410, 431, 512, 527, 545
RsaNI	GTAC	8 cut(s)	121, 127, 400, 409, 430, 511, 526, 544
Rsr2I	CGGWCCG	2 cut(s)	146, 200
RsrII	CGGWCCG	2 cut(s)	146, 200
SaqAI	TTAA	1 cut(s)	153
SatI	GCNGC	3 cut(s)	87, 361, 573
Sau3AI	GATC	1 cut(s)	253
Sau96I	GGNCC	5 cut(s)	146, 200, 286, 516, 534
SchI	GAGTC	1 cut(s)	278
ScrFI	CCNGG	2 cut(s)	159, 326
SduI	GDGCHC	1 cut(s)	374
SetI	ASST	9 cut(s)	60, 84, 99, 264, 285, 297, 351, 615, 633
SfaNI	GCATC	1 cut(s)	26
SfcI	CTRYAG	1 cut(s)	27
Sfr274I	CTCGAG	1 cut(s)	112
SinI	GGWCC	4 cut(s)	146, 200, 286, 516
SlaI	CTCGAG	1 cut(s)	112
SmlI	CTYRAG	2 cut(s)	59, 112
SmoI	CTYRAG	2 cut(s)	59, 112
Sse9I	AATT	3 cut(s)	71, 236, 245
SsiI	CCGC	7 cut(s)	86, 149, 360, 422, 467, 572, 623
SspI	AATATT	1 cut(s)	385
StyD4I	CCNGG	2 cut(s)	157, 324
StyI	CCWWGG	1 cut(s)	609
TaaI	ACNGT	8 cut(s)	37, 172, 184, 193, 290, 391, 505, 653
TaiI	ACGT	3 cut(s)	99, 297, 633
TaqI	TCGA	3 cut(s)	113, 252, 320
TasI	AATT	3 cut(s)	71, 236, 245
TatI	WGTACW	2 cut(s)	399, 525
TauI	GCSGC	3 cut(s)	89, 363, 575
TfiI	GAWTC	2 cut(s)	8, 589
Tru1I	TTAA	1 cut(s)	153
Tru9I	TTAA	1 cut(s)	153
TscAI	CASTG	2 cut(s)	553, 672
TseFI	GTSAC	1 cut(s)	290
Tsp45I	GTSAC	1 cut(s)	290
TspGWI	ACGGA	2 cut(s)	556, 611
TspRI	CASTG	2 cut(s)	553, 672
VpaK11BI	GGWCC	4 cut(s)	146, 200, 286, 516
XapI	RAATTY	2 cut(s)	71, 245
XhoI	CTCGAG	1 cut(s)	112
XmiI	GTMKAC	2 cut(s)	26, 207
XmnI	GAANNNNTTC	1 cut(s)	75

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.