AT3G03570

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007197
Physical Location & Seq
Forward (+)
856876 .. 860469
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03570.1

Sequence Viewer

Length: 1824 bp
ATGTCGCAGCAAATCAGCGGTAATAATAATATCCCGCTGAGTGAAGTTTACTGGTCTCTCGTTAACAAAGCCGATAAGAAATTCTCCAAGATCAGAGACTTGCCCTTCTATGAACGCTCCAGGTATGAGAACTATTTCTTCAAGGTATTCAAAGTATACACACAACTATGGAAGTTTCAGCAAGAGAATAGGCAGAAGCTTGTTGAAGCTGGACTTAAGAGATGGGAGATTGGAGAGATCGCTTCTCGCATTGCCCAGCTTTACTATGGACATTATATGCGAACAAGTGATGCTGGTTACTTATCCGAGTCCTATGTATTCTACGAAGCAATACTCACTAGGGAGTACTTCAAAGATGGATTGTTTCAGGATCTCAATATTGCAAACAAGCAGCTTCGGTTTCTTGCAAGGTTTCTTATGGTGTGTTTGGTTCTTGGCCGCCGTGAGATGGTGCATCAACTCGTTGATCAGTTTAAACGGTTGATTGATGAATGCAAGAGGACATTCCAGGAAACCGATTTTAAGGAATGGAAGGTGGTGGCGCAGGAAATAGTTAGATTTCTGAAATCGGACACTGCATTCATGAACATTAGACCTCTAAGATACAGTCTTGTATTGGATCCTAATCTGGATGCTGGTACTCCGCGTGCAAGTAGGTCTTTAAGGTTGACGGATGCTATCTTGAGCAGTTATTACTGTAACGAGGTCAAGTATTCGGAGCTCACGCTCGATTCTTTTAGGATGCTTCAATGCTTAGAGTGGGAACCGAGTGGTTCACTATATCAATCAACTGGTGCTAAAATGGGTCAGAATGCTCCCGTTGGAGTTGCTCGTATTAACTCCCAGAGCATGAATGATCCTACTTTGCCACCTAACCCTCGCAAAGCTGTCCTTTATCGTCCATCCATAACACATTTTTTGGCTGTTTTGGCCACCATTTGTGAGGAGCTTCCTTCTCATGGCATTCTTTTATTATATCTGTCTGCTTCTGGCAAGATTGGACAGATTTCATCATCTCCCTTGAGTGCTAGAAGTGCAACCAGTGTTGAGGAGAATATCTTGAGAGACTTTGAATCTCACACAATCAAACAAGAGACAGAACCATCACTTCAAATTACGCCATCTGGTCAGTCTTTGAGGCAAATCAGTGAGGACGCAGTCAGTACCCCATGTGGTTTGTCTTTTGGCAGTCATGGGCTCACAGGATCAAGCTACATATATCCCTCTGATTTAGTTCCATTTACAAGGAAACCTCTTTTCATAATCATTGACAGTGATAGCAGCACAGTTTTTAAAAATATTTGTGGAGCGGAGAAAGGAGAACCAGCCGCGTTACTTCTGTCTCCATCACATACTCCTCCGCTTATCTCTGCTGATTTCTCTCGTCAACCTAGTGGTAGCCTTTTCACCATTTTTCTCACGTCACCAGTCCAAGCGTTTTGCTTGTTAAGTGAGATTTCCGCCTCTGATATGGAAACGGATATTTTTACTAAGGCCGAGAAGCTTCTTTCCTCGTCTATGAACGAATGGGCTTCGACATTGGCAACATCAGATACCCTTCACCCTGTTTGGTCACAGATACTAAAAGATCCCTTCTTGAGACGACTACTCCTAAGATTCATATTCTGCCGGGCTGTCTTGGCACTGTACACTCCAGTCTTCAATAACAAACAAAATCAGCCAGAGTGCTGTCCGTCTCTCCCAGAATCGCTATTACCAACTGCTCCTGCTGTTCAATCCGCTGTGTTTCAGATGGCCAATGTATTTGGTGCTACAAGTAAATTTACTATACCTCAAGATATCACAATGCTCGAAAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

607

Amino Acids

68.63

Weight (kDa)

7.51

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 19 - 552 1.8e-201 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1310
AccI GTMKAC 1 cut(s) 156
AccII CGCG 2 cut(s) 646, 1331
AciI CCGC 9 cut(s) 18, 35, 439, 644, 1310, 1329, 1361, 1461, 1740
AclWI GGATC 6 cut(s) 378, 614, 627, 851, 1213, 1583
AcoI YGGCCR 3 cut(s) 436, 930, 1755
AcsI RAATTY 2 cut(s) 80, 1781
AfaI GTAC 4 cut(s) 347, 640, 1165, 1649
AfiI CCNNNNNNNGG 2 cut(s) 448, 959
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 9 cut(s) 142, 151, 206, 352, 749, 1073, 1112, 1663, 1736
AjiI CACGTC 1 cut(s) 1422
AjnI CCWGG 2 cut(s) 119, 507
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 7 cut(s) 60, 90, 1059, 1088, 1347, 1594, 1701
AlwI GGATC 6 cut(s) 378, 614, 627, 851, 1213, 1583
AoxI GGCC 4 cut(s) 436, 930, 1494, 1755
ApeKI GCWGC 3 cut(s) 7, 391, 1281
ApoI RAATTY 2 cut(s) 80, 1781
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 544
AsuC2I CCSGG 1 cut(s) 1631
AsuHPI GGTGA 3 cut(s) 1399, 1416, 1553
BalI TGGCCA 2 cut(s) 932, 1757
BamHI GGATCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 723, 1200
BbsI GAAGAC 1 cut(s) 1651
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 3 cut(s) 19, 403, 1293
BccI CCATC 8 cut(s) 216, 350, 442, 910, 1111, 1129, 1354, 1747
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 2 cut(s) 121, 509
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 1631
BcoDI GTCTC 7 cut(s) 60, 90, 1059, 1088, 1347, 1594, 1701
BfaI CTAG 3 cut(s) 339, 1029, 1392
BfrI CTTAAG 1 cut(s) 215
BisI GCNGC 5 cut(s) 8, 392, 439, 1282, 1329
BlsI GCNGC 5 cut(s) 9, 393, 440, 1283, 1330
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 121, 509, 1631
BmgBI CACGTC 1 cut(s) 1422
BmiI GGNNCC 2 cut(s) 621, 765
BmrFI CCNGG 3 cut(s) 121, 509, 1631
BmsI GCATC 5 cut(s) 280, 463, 622, 664, 732
BpiI GAAGAC 1 cut(s) 1651
BpmI CTGGAG 2 cut(s) 103, 1638
BpuEI CTTGAG 5 cut(s) 703, 1042, 1081, 1618, 1779
BpuMI CCSGG 1 cut(s) 1631
BsaBI GATNNNNATC 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 1593, 1623
Bsc4I CCNNNNNNNGG 2 cut(s) 448, 959
Bse1I ACTGG 5 cut(s) 56, 796, 1041, 1427, 1655
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 2 cut(s) 121, 509
BseGI GGATG 4 cut(s) 637, 679, 747, 902
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 2 cut(s) 448, 959
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 5 cut(s) 56, 796, 1041, 1427, 1655
BseRI GAGGAG 3 cut(s) 959, 1064, 1347
BseXI GCAGC 3 cut(s) 19, 403, 1293
BseYI CCCAGC 1 cut(s) 255
Bsh1236I CGCG 2 cut(s) 646, 1331
BshFI GGCC 4 cut(s) 438, 932, 1496, 1757
BsiHKAI GWGCWC 1 cut(s) 723
BsiSI CCGG 1 cut(s) 1630
BslI CCNNNNNNNGG 2 cut(s) 448, 959
BsmAI GTCTC 7 cut(s) 60, 90, 1059, 1088, 1347, 1594, 1701
BsmBI CGTCTC 2 cut(s) 1594, 1701
BsmI GAATGC 4 cut(s) 497, 578, 817, 963
BsnI GGCC 4 cut(s) 438, 932, 1496, 1757
Bso31I GGTCTC 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 723, 1200
Bsp1407I TGTACA 1 cut(s) 1647
Bsp143I GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
BspACI CCGC 9 cut(s) 18, 35, 439, 644, 1310, 1329, 1361, 1461, 1740
BspANI GGCC 4 cut(s) 438, 932, 1496, 1757
BspCNI CTCAG 1 cut(s) 30
BspFNI CGCG 2 cut(s) 646, 1331
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 2 cut(s) 621, 765
BspPI GGATC 6 cut(s) 378, 614, 627, 851, 1213, 1583
BspTI CTTAAG 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 60
BsrBI CCGCTC 1 cut(s) 1310
BsrDI GCAATG 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 1647
BsrI ACTGG 5 cut(s) 56, 796, 1041, 1427, 1655
BssMI GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
BssNAI GTATAC 1 cut(s) 157
Bst1107I GTATAC 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 121, 509
Bst4CI ACNGT 6 cut(s) 480, 608, 698, 1274, 1288, 1647
BstAFI CTTAAG 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 1647
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 5 cut(s) 38, 599, 754, 1491, 1613
BstF5I GGATG 4 cut(s) 637, 679, 747, 902
BstFNI CGCG 2 cut(s) 646, 1331
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 8 cut(s) 93, 240, 373, 469, 622, 859, 1208, 1591
BstMAI GTCTC 7 cut(s) 60, 90, 1059, 1088, 1347, 1594, 1701
BstMBI GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
BstMWI GCNNNNNNNGC 3 cut(s) 929, 1034, 1640
BstNI CCWGG 2 cut(s) 121, 509
BstSCI CCNGG 3 cut(s) 119, 507, 1629
BstUI CGCG 2 cut(s) 646, 1331
BstV1I GCAGC 3 cut(s) 19, 403, 1293
BstV2I GAAGAC 1 cut(s) 1651
BstX2I RGATCY 3 cut(s) 370, 619, 1588
BstYI RGATCY 3 cut(s) 370, 619, 1588
BstZ17I GTATAC 1 cut(s) 157
BsuRI GGCC 4 cut(s) 438, 932, 1496, 1757
BtrI CACGTC 1 cut(s) 1422
BtsCI GGATG 4 cut(s) 637, 679, 747, 902
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 5 cut(s) 573, 1048, 1153, 1279, 1643
Cac8I GCNNGC 1 cut(s) 648
CciI TCATGA 1 cut(s) 582
CfoI GCGC 1 cut(s) 544
CseI GACGC 1 cut(s) 1163
Csp6I GTAC 4 cut(s) 346, 639, 1164, 1648
CviAII CATG 5 cut(s) 583, 850, 959, 1170, 1193
CviQI GTAC 4 cut(s) 346, 639, 1164, 1648
DdeI CTNAG 5 cut(s) 38, 599, 754, 1491, 1613
DpnI GATC 8 cut(s) 92, 239, 372, 468, 621, 858, 1207, 1590
DpnII GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
DraI TTTAAA 2 cut(s) 475, 1294
EaeI YGGCCR 3 cut(s) 436, 930, 1755
EciI GGCGGA 1 cut(s) 1450
Ecl136II GAGCTC 1 cut(s) 721
Eco24I GRGCYC 2 cut(s) 723, 1200
Eco31I GGTCTC 1 cut(s) 60
Eco32I GATATC 1 cut(s) 1801
Eco53kI GAGCTC 1 cut(s) 721
EcoICRI GAGCTC 1 cut(s) 721
EcoRII CCWGG 2 cut(s) 119, 507
EcoRV GATATC 1 cut(s) 1801
EcoT38I GRGCYC 2 cut(s) 723, 1200
Esp3I CGTCTC 2 cut(s) 1594, 1701
FaeI CATG 5 cut(s) 586, 853, 962, 1173, 1196
FalI AAGNNNNNCTT 6 cut(s) 198, 230, 643, 675, 876, 908
FatI CATG 5 cut(s) 582, 849, 958, 1169, 1192
FauI CCCGC 1 cut(s) 42
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 5 cut(s) 8, 392, 439, 1282, 1329
FokI GGATG 4 cut(s) 644, 686, 754, 889
FriOI GRGCYC 2 cut(s) 723, 1200
Fsp4HI GCNGC 5 cut(s) 8, 392, 439, 1282, 1329
FspBI CTAG 3 cut(s) 339, 1029, 1392
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 8, 392, 439, 1282, 1329
GsaI CCCAGC 1 cut(s) 259
GsuI CTGGAG 2 cut(s) 103, 1638
HaeIII GGCC 4 cut(s) 438, 932, 1496, 1757
HapII CCGG 1 cut(s) 1630
HgaI GACGC 1 cut(s) 1163
HhaI GCGC 1 cut(s) 544
Hin1II CATG 5 cut(s) 586, 853, 962, 1173, 1196
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 3 cut(s) 64, 669, 1388
HindII GTYRAC 3 cut(s) 64, 669, 1388
HindIII AAGCTT 3 cut(s) 197, 1502, 1816
HinfI GANTC 5 cut(s) 308, 731, 1073, 1617, 1706
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 1 cut(s) 1630
HphI GGTGA 3 cut(s) 1399, 1416, 1553
Hpy166II GTNNAC 7 cut(s) 49, 64, 157, 669, 776, 1388, 1650
Hpy188III TCNNGA 7 cut(s) 368, 583, 629, 682, 1060, 1597, 1796
Hpy8I GTNNAC 7 cut(s) 49, 64, 157, 669, 776, 1388, 1650
HpyAV CCTTC 5 cut(s) 115, 526, 963, 1568, 1603
HpyCH4III ACNGT 6 cut(s) 480, 608, 698, 1274, 1288, 1647
HpyCH4IV ACGT 1 cut(s) 1421
HpyCH4V TGCA 7 cut(s) 383, 407, 454, 495, 578, 650, 1037
HpyF10VI GCNNNNNNNGC 3 cut(s) 929, 1034, 1640
HpyF3I CTNAG 5 cut(s) 38, 599, 754, 1491, 1613
HpySE526I ACGT 1 cut(s) 1421
Hsp92II CATG 5 cut(s) 586, 853, 962, 1173, 1196
HspAI GCGC 1 cut(s) 542
Ksp22I TGATCA 1 cut(s) 466
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
LmnI GCTCC 6 cut(s) 122, 718, 820, 946, 1307, 1729
Lsp1109I GCAGC 3 cut(s) 19, 403, 1293
LweI GCATC 5 cut(s) 280, 463, 622, 664, 732
MaeI CTAG 3 cut(s) 339, 1029, 1392
MaeII ACGT 1 cut(s) 1421
MaeIII GTNAC 5 cut(s) 296, 698, 1332, 1422, 1572
MalI GATC 8 cut(s) 92, 239, 372, 468, 621, 858, 1207, 1590
MbiI CCGCTC 1 cut(s) 1310
MboI GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
MboII GAAGA 2 cut(s) 130, 1651
MflI RGATCY 3 cut(s) 370, 619, 1588
MhlI GDGCHC 2 cut(s) 723, 1200
MlsI TGGCCA 2 cut(s) 932, 1757
MluCI AATT 3 cut(s) 80, 1113, 1781
MluNI TGGCCA 2 cut(s) 932, 1757
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 802
Mox20I TGGCCA 2 cut(s) 932, 1757
MroXI GAANNNNTTC 1 cut(s) 134
MscI TGGCCA 2 cut(s) 932, 1757
MseI TTAA 8 cut(s) 63, 216, 474, 522, 662, 837, 1293, 1448
MslI CAYNNNNRTG 1 cut(s) 166
Msp20I TGGCCA 2 cut(s) 932, 1757
MspA1I CMGCKG 3 cut(s) 18, 37, 1742
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 1630
MspR9I CCNGG 3 cut(s) 121, 509, 1631
MssI GTTTAAAC 1 cut(s) 475
Mva1269I GAATGC 4 cut(s) 497, 578, 817, 963
MvaI CCWGG 2 cut(s) 121, 509
MvnI CGCG 2 cut(s) 646, 1331
MwoI GCNNNNNNNGC 3 cut(s) 929, 1034, 1640
NciI CCSGG 1 cut(s) 1631
NdeII GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
NlaIII CATG 5 cut(s) 586, 853, 962, 1173, 1196
NlaIV GGNNCC 2 cut(s) 621, 765
NmeAIII GCCGAG 1 cut(s) 1522
NmuCI GTSAC 2 cut(s) 1422, 1572
PagI TCATGA 1 cut(s) 582
PctI GAATGC 4 cut(s) 497, 578, 817, 963
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 4 cut(s) 731, 1073, 1617, 1706
PflFI GACNNNGTC 1 cut(s) 1157
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 5 cut(s) 9, 393, 440, 1283, 1330
PleI GAGTC 1 cut(s) 316
PmeI GTTTAAAC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 119, 507
PspFI CCCAGC 1 cut(s) 255
PspGI CCWGG 2 cut(s) 119, 507
PspN4I GGNNCC 2 cut(s) 621, 765
PsuI RGATCY 3 cut(s) 370, 619, 1588
PsyI GACNNNGTC 1 cut(s) 1157
RsaI GTAC 4 cut(s) 347, 640, 1165, 1649
RsaNI GTAC 4 cut(s) 346, 639, 1164, 1648
RseI CAYNNNNRTG 1 cut(s) 166
SacI GAGCTC 1 cut(s) 723
SaqAI TTAA 8 cut(s) 63, 216, 474, 522, 662, 837, 1293, 1448
SatI GCNGC 5 cut(s) 8, 392, 439, 1282, 1329
Sau3AI GATC 8 cut(s) 90, 237, 370, 466, 619, 856, 1205, 1588
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 3 cut(s) 121, 509, 1631
SduI GDGCHC 2 cut(s) 723, 1200
SfaNI GCATC 5 cut(s) 280, 463, 622, 664, 732
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 6 cut(s) 215, 682, 1021, 1060, 1597, 1794
SmoI CTYRAG 6 cut(s) 215, 682, 1021, 1060, 1597, 1794
Sse9I AATT 3 cut(s) 80, 1113, 1781
SsiI CCGC 9 cut(s) 18, 35, 439, 644, 1310, 1329, 1361, 1461, 1740
SspI AATATT 2 cut(s) 379, 1300
SspMI CTAG 3 cut(s) 339, 1029, 1392
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 3 cut(s) 119, 507, 1629
TaaI ACNGT 6 cut(s) 480, 608, 698, 1274, 1288, 1647
TaiI ACGT 1 cut(s) 1424
TaqI TCGA 3 cut(s) 729, 1535, 1812
TasI AATT 3 cut(s) 80, 1113, 1781
TatI WGTACW 2 cut(s) 345, 1647
TauI GCSGC 2 cut(s) 441, 1331
TfiI GAWTC 4 cut(s) 731, 1073, 1617, 1706
Tru1I TTAA 8 cut(s) 63, 216, 474, 522, 662, 837, 1293, 1448
Tru9I TTAA 8 cut(s) 63, 216, 474, 522, 662, 837, 1293, 1448
TscAI CASTG 5 cut(s) 580, 1048, 1153, 1279, 1650
TseFI GTSAC 2 cut(s) 1422, 1572
TseI GCWGC 3 cut(s) 7, 391, 1281
Tsp45I GTSAC 2 cut(s) 1422, 1572
TspDTI ATGAA 9 cut(s) 126, 504, 571, 599, 866, 999, 1249, 1535, 1609
TspGWI ACGGA 3 cut(s) 686, 1493, 1683
TspRI CASTG 5 cut(s) 580, 1048, 1153, 1279, 1650
Tth111I GACNNNGTC 1 cut(s) 1157
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 2 cut(s) 80, 1781
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 3 cut(s) 339, 1029, 1392
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.