RLG00000029489

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007197
Physical Location & Seq
Forward (+)
39448896 .. 39456129
7234 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029489

Sequence Viewer

Length: 945 bp
ATGCTGCAAAGATACAGTGGAATGCCCTGTTTTAGCTTGTCATTGATTTTCTGTTGCTGCTGTTGTGGTGATGGGCCATTGAGATATAGTATTGTGCTGGAATGTCATCCTGATTCCTTACCACCTGTTTCCACATTGGTTGTAAGGAGGTCTTTGAGACTGCGAGATGCGATACTAAGCAGCTACCATCATAATGAGGTTCTGGCAACAGTTTGTGAGGAGCTTCCATCTGATCGAGTTATCTTGATATATCTGTCCGCTGCAGCCGGTTCTGGAATGTACGCTGATGCAACATCTGACAGACTAAATCCTTCTTGTGGACAAAGAAAAGGAGATGACTCAAGCTTACAGGCTAGTTGTTTAAACTTTGGTAGCCATGGAAATGGAGGAATAAATAGCATCCATCCCAGTGACTTGGTTCCGTATACAAGAAGGCCGCTCTTTCTGGTTATTGACTGTGACAGCAGTGAAGCTTTTGAGGCTATAAATGGAGTACAAAAAGGAGAGACAGCTGCAATGCTCCTATCTTCGAGCTCTTCATATCAAGCTTCTGATGTTGGTTCCTCTCGTCAGCCAAGTGGAAGCTTATTCACTATTTTCCTTACAGCTCCAATGCAGGCTTTCTGTTTATTGCTTGGTGTTTCAGGCACTGATATTGACATGGTTTTGACTGATCCATTCCTGAGGCGACTCCTATTGAGGTTCATCTTTTGTCGGGCGGTGCTATCACTCTATGCTCCAACCTTCAATAAGAAGGAATTTATTCCCAAGTGTAAGGACTTGTACTCCCAGAAAATAGACAGGGTGACACAGAAGCAGTGCCATGTTCATGAATCATACCGAGTAAAGCAGAAGAAGGACTTGTACTCCCAGAAAATAGACAGGGTGACACAGAAGCAGTGCCATGTTCATGAATCATACCGAGTAAAGCAGAAGGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

315

Amino Acids

34.96

Weight (kDa)

8.43

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 67 - 220 6e-34 Protein SCAI
SCAI PF12070 221 - 247 1.2e-06 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 425
AciI CCGC 3 cut(s) 258, 437, 719
AclWI GGATC 1 cut(s) 668
AcsI RAATTY 1 cut(s) 758
AfaI GTAC 4 cut(s) 281, 495, 785, 866
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 1 cut(s) 748
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 2 cut(s) 151, 500
AlwI GGATC 1 cut(s) 668
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 2 cut(s) 74, 434
ApeKI GCWGC 6 cut(s) 4, 57, 180, 260, 263, 512
ApoI RAATTY 1 cut(s) 758
Asp700I GAANNNNTTC 1 cut(s) 762
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 80, 817, 898
AxyI CCTNAGG 1 cut(s) 683
BanII GRGCYC 1 cut(s) 536
BarI GAAGNNNNNNTAC 2 cut(s) 848, 880
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 5 cut(s) 44, 192, 247, 275, 499
BccI CCATC 4 cut(s) 65, 195, 235, 411
BcoDI GTCTC 2 cut(s) 151, 500
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 7 cut(s) 5, 58, 181, 261, 264, 437, 513
BlsI GCNGC 7 cut(s) 6, 59, 182, 262, 265, 438, 514
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 420, 562
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 3 cut(s) 157, 277, 408
BmuI ACTGGG 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 376
BsaXI ACNNNNNCTCC 4 cut(s) 770, 800, 851, 881
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 408
Bse21I CCTNAGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 3 cut(s) 106, 399, 403
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 5 cut(s) 44, 192, 247, 275, 499
BshFI GGCC 2 cut(s) 76, 436
BsiHKAI GWGCWC 1 cut(s) 536
BsiSI CCGG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 2 cut(s) 151, 500
BsmI GAATGC 1 cut(s) 27
BsnI GGCC 2 cut(s) 76, 436
Bsp1286I GDGCHC 1 cut(s) 536
Bsp143I GATC 2 cut(s) 232, 673
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 3 cut(s) 258, 437, 719
BspANI GGCC 2 cut(s) 76, 436
BspCNI CTCAG 1 cut(s) 675
BspHI TCATGA 2 cut(s) 829, 910
BspLI GGNNCC 2 cut(s) 420, 562
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 668
BspQI GCTCTTC 1 cut(s) 541
BsrBI CCGCTC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 2 cut(s) 232, 673
BssNAI GTATAC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 376
Bst1107I GTATAC 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 17, 211, 458
Bst6I CTCTTC 1 cut(s) 541
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 2 cut(s) 176, 683
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 3 cut(s) 106, 399, 403
BstKTI GATC 2 cut(s) 235, 676
BstMAI GTCTC 2 cut(s) 151, 500
BstMBI GATC 2 cut(s) 232, 673
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 5 cut(s) 44, 192, 247, 275, 499
BstXI CCANNNNNNTGG 2 cut(s) 383, 415
BstZ17I GTATAC 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 683
BsuRI GGCC 2 cut(s) 76, 436
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 3 cut(s) 106, 399, 403
BtsI GCAGTG 3 cut(s) 472, 824, 905
BtsIMutI CAGTG 6 cut(s) 22, 415, 472, 648, 824, 905
Cac8I GCNNGC 1 cut(s) 618
CaiI CAGNNNCTG 1 cut(s) 650
CciI TCATGA 2 cut(s) 829, 910
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 4 cut(s) 280, 494, 784, 865
CspCI CAANNNNNGTGG 2 cut(s) 121, 156
CviAII CATG 6 cut(s) 377, 661, 824, 830, 905, 911
CviQI GTAC 4 cut(s) 280, 494, 784, 865
DdeI CTNAG 2 cut(s) 176, 683
DpnI GATC 2 cut(s) 234, 675
DpnII GATC 2 cut(s) 232, 673
DraI TTTAAA 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 541
EarI CTCTTC 1 cut(s) 541
Ecl136II GAGCTC 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 534
Eco81I CCTNAGG 1 cut(s) 683
EcoICRI GAGCTC 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 6 cut(s) 380, 664, 827, 833, 908, 914
FalI AAGNNNNNCTT 4 cut(s) 136, 168, 845, 877
FatI CATG 6 cut(s) 376, 660, 823, 829, 904, 910
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 7 cut(s) 5, 58, 181, 261, 264, 437, 513
FokI GGATG 3 cut(s) 93, 386, 390
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 7 cut(s) 5, 58, 181, 261, 264, 437, 513
FspBI CTAG 1 cut(s) 354
GluI GCNGC 7 cut(s) 5, 58, 181, 261, 264, 437, 513
HaeIII GGCC 2 cut(s) 76, 436
HapII CCGG 1 cut(s) 267
Hin1II CATG 6 cut(s) 380, 664, 827, 833, 908, 914
HindIII AAGCTT 4 cut(s) 343, 471, 546, 583
HinfI GANTC 5 cut(s) 113, 338, 690, 833, 914
HpaII CCGG 1 cut(s) 267
HphI GGTGA 3 cut(s) 80, 817, 898
Hpy166II GTNNAC 2 cut(s) 320, 426
Hpy188I TCNGA 3 cut(s) 232, 298, 553
Hpy188III TCNNGA 6 cut(s) 110, 244, 273, 682, 830, 911
Hpy8I GTNNAC 2 cut(s) 320, 426
HpyAV CCTTC 6 cut(s) 321, 426, 748, 754, 850, 928
HpyCH4III ACNGT 3 cut(s) 17, 211, 458
HpyCH4V TGCA 5 cut(s) 7, 263, 290, 515, 616
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 2 cut(s) 176, 683
Hsp92II CATG 6 cut(s) 380, 664, 827, 833, 908, 914
Kzo9I GATC 2 cut(s) 232, 673
LguI GCTCTTC 1 cut(s) 541
LmnI GCTCC 4 cut(s) 220, 525, 613, 742
Lsp1109I GCAGC 5 cut(s) 44, 192, 247, 275, 499
LweI GCATC 3 cut(s) 157, 277, 408
MaeI CTAG 1 cut(s) 354
MaeIII GTNAC 4 cut(s) 410, 458, 805, 886
MalI GATC 2 cut(s) 234, 675
MbiI CCGCTC 1 cut(s) 439
MboI GATC 2 cut(s) 232, 673
MboII GAAGA 3 cut(s) 519, 528, 865
MhlI GDGCHC 1 cut(s) 536
MluCI AATT 1 cut(s) 758
MlyI GAGTC 2 cut(s) 332, 684
MmeI TCCRAC 1 cut(s) 764
MnlI CCTC 8 cut(s) 141, 190, 211, 380, 472, 574, 678, 693
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 5 cut(s) 192, 381, 408, 828, 909
MspA1I CMGCKG 2 cut(s) 260, 512
MspI CCGG 1 cut(s) 267
MssI GTTTAAAC 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 479
NcoI CCATGG 1 cut(s) 376
NdeII GATC 2 cut(s) 232, 673
NlaIII CATG 6 cut(s) 380, 664, 827, 833, 908, 914
NlaIV GGNNCC 2 cut(s) 420, 562
NmuCI GTSAC 4 cut(s) 410, 458, 805, 886
PagI TCATGA 2 cut(s) 829, 910
PciSI GCTCTTC 1 cut(s) 541
PctI GAATGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 3 cut(s) 113, 833, 914
PkrI GCNGC 7 cut(s) 6, 59, 182, 262, 265, 438, 514
PleI GAGTC 2 cut(s) 332, 684
PmeI GTTTAAAC 1 cut(s) 363
PpsI GAGTC 2 cut(s) 332, 684
Psp124BI GAGCTC 1 cut(s) 536
PspN4I GGNNCC 2 cut(s) 420, 562
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 650
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 4 cut(s) 281, 495, 785, 866
RsaNI GTAC 4 cut(s) 280, 494, 784, 865
RseI CAYNNNNRTG 5 cut(s) 192, 381, 408, 828, 909
SacI GAGCTC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 541
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 7 cut(s) 5, 58, 181, 261, 264, 437, 513
Sau3AI GATC 2 cut(s) 232, 673
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 2 cut(s) 332, 684
SduI GDGCHC 1 cut(s) 536
SfaNI GCATC 3 cut(s) 157, 277, 408
SfcI CTRYAG 1 cut(s) 261
SmiMI CAYNNNNRTG 5 cut(s) 192, 381, 408, 828, 909
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 1 cut(s) 758
SsiI CCGC 3 cut(s) 258, 437, 719
SspMI CTAG 1 cut(s) 354
SstI GAGCTC 1 cut(s) 536
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 3 cut(s) 17, 211, 458
TaqI TCGA 2 cut(s) 235, 530
TasI AATT 1 cut(s) 758
TatI WGTACW 3 cut(s) 493, 783, 864
TauI GCSGC 1 cut(s) 439
TfiI GAWTC 3 cut(s) 113, 833, 914
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 6 cut(s) 22, 415, 472, 655, 824, 905
TseFI GTSAC 4 cut(s) 410, 458, 805, 886
TseI GCWGC 6 cut(s) 4, 57, 180, 260, 263, 512
Tsp45I GTSAC 4 cut(s) 410, 458, 805, 886
TspDTI ATGAA 6 cut(s) 528, 694, 818, 846, 899, 927
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 6 cut(s) 22, 415, 472, 655, 824, 905
XapI RAATTY 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.