pycom02g20430

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007197
Physical Location & Seq
Reverse (-)
18542744 .. 18547658
4915 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20430.1

Sequence Viewer

Length: 1575 bp
ATGCAAGCACAGCAAAACCCTAACATCCCTGTTAGCGAAATCTACTGGTCTCTCCTCCACAAAGCTGACAAGAAATTCTCCAAAATCAGAGAGCTCCCCTATTACCACCGCAACAGGCACGAGTACGACGCGTATTTCTACAAGGTCTTCAAGGTGTACACGCAGCTATGGAAATTTCAGCAAGAAAATCGCCAGAAACTCGTGGAGTCCGGTCTCCGGCGATCGGAGATTGGCGAGATCGCGTCGCGAATTGCGCAGCTCTACTTGGGGCAGTACATGAGGACCAGCGAGGCAAGCTACCTCTCCGAGGCGTACATATTCTACGAGGCGATTCTCAGCAGAGAGTACTTCAAGGAGGGCATGTTTCAGGACCTGAGCTTGGCCAACAAGCAGCTCCGGTTTGTTTCGAGGTTTTTAATGGTGTGCTTGCTCTTGAATCGGCGAGAGATGGTGCAGCAGCTGCTCAATCAGCTCAGAGTCTTGGTTGATGAGTGCAAGAGGACCTTCCAGGAAACTGATTTTAAAGAATGGAAGCTGGTGATTCAGGAAATAGTTAGATTTTTGAAAGCTGACACAGCTTTTATGAATATCAGGCCTTTGAGATACAGTATTGTTCTAGACTGTCATCCTGATTCCCTGCCACATGTTTCCACAGCAGTTGCAAAGAGGTCCTTGAGACTGCGAGATGCTATACTGAGCAGCTACCATCATAATGAGGTAAAGTTTTCAGAGCTTACTCTTGACACTTTTAGGATGCTTCAGTGTCTGGAGTGGGAACCTAGTGGCTCATTTTACCAGTCTAGTGGGGCTAAAAATGGTCAGAATGGGGCACCGGCGTCCAGCCGCATTAATTACTCTCAAGATATTGCCGATCCTACTCTGCCACCTAATCCAAGAAAGGCTGTCTTATACCGTCCGTCTGTTACACATTTTCTAGCAGTTTTGGCCACAGTTTGTGAGGAGCTTCCCCCTGATGGAGTTGTTTTGATATATCTGTCAGCTTCAGGTGCTGGAATGTACTCTGATGCGACGTCCCCTTCTACATTGGCCTATTCGTCTGATAGGATAAACCCTTCTCGTGGACAAGGTAAAGGAGATGGTTCAAGCTCTCAGGAGGGCTGTTTACAGTTTGGTAGCCATAGAAACGGAGGAGTGAATAACATTTATCCGAGTGACTTGGTTCCATTTACACGAAGACCACTTGTTTTAGTTATTGACTGTGACAGCAGTGAAGCTTTTGAGGCTATCAACGGAGTACAAAAAGGAGAGACCGCTGCAATGCTCCTATCTTCTAGCTCCTCATATCACACTACTCTCGGTTCCTCTCGTCAGTCAAGCGGAAGCCTATTCACTATTTTCCTCACAGCTCCAGTGCAGGCTTTCTGTTTACTGCTTGGTATTTCAGGTTCTGACATTGACATGGAAACATTTGACAAGGCAGAGAAGCTTCTTTCCAGTTCGTTAAGTGAATGGGGGTCTACTTTGGTGACATTGGACACTATTGATCCTGTTTGGGCACAAATTTTGAGTGACCCCTTCCTAAGGCGACTCCTTTTGAGGTACTCCTCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

525

Amino Acids

59.37

Weight (kDa)

7.59

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 18 - 521 3.4e-189 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1034
Acc16I TGCGCA 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 829
AccI GTMKAC 1 cut(s) 1478
AccII CGCG 3 cut(s) 131, 242, 247
AciI CCGC 4 cut(s) 109, 844, 1272, 1338
AclWI GGATC 2 cut(s) 866, 1499
AcoI YGGCCR 2 cut(s) 381, 945
AcsI RAATTY 3 cut(s) 74, 173, 1521
AcuI CTGAAG 2 cut(s) 743, 987
AcyI GRCGYC 2 cut(s) 836, 1031
AfaI GTAC 8 cut(s) 125, 158, 275, 314, 347, 1019, 1257, 1562
AfiI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
AflIII ACRYGT 2 cut(s) 129, 643
AgsI TTSAA 5 cut(s) 151, 352, 436, 565, 1104
AjnI CCWGG 1 cut(s) 507
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 4 cut(s) 54, 218, 670, 1262
AlwI GGATC 2 cut(s) 866, 1499
AlwNI CAGNNNCTG 5 cut(s) 373, 460, 766, 1010, 1409
AoxI GGCC 4 cut(s) 381, 593, 945, 1047
ApeKI GCWGC 8 cut(s) 163, 256, 391, 454, 457, 460, 699, 1274
ApoI RAATTY 3 cut(s) 74, 173, 1521
AseI ATTAAT 1 cut(s) 849
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 4 cut(s) 282, 370, 501, 669
AsuHPI GGTGA 2 cut(s) 550, 1498
AvaII GGWCC 4 cut(s) 282, 370, 501, 669
AxyI CCTNAGG 1 cut(s) 1541
BaeGI GKGCMC 2 cut(s) 832, 1519
BalI TGGCCA 2 cut(s) 383, 947
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 3 cut(s) 119, 200, 1077
BbsI GAAGAC 2 cut(s) 139, 1201
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 8 cut(s) 175, 268, 403, 447, 466, 469, 711, 1261
BccI CCATC 4 cut(s) 442, 714, 968, 1091
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 4 cut(s) 54, 218, 670, 1262
BfaI CTAG 5 cut(s) 617, 780, 801, 935, 1293
BisI GCNGC 9 cut(s) 164, 257, 392, 455, 458, 461, 700, 844, 1275
BlsI GCNGC 9 cut(s) 165, 258, 393, 456, 459, 462, 701, 845, 1276
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 4 cut(s) 282, 370, 501, 669
BmgT120I GGNCC 4 cut(s) 282, 370, 501, 669
BmiI GGNNCC 4 cut(s) 777, 831, 1182, 1321
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 3 cut(s) 676, 744, 1015
BpiI GAAGAC 2 cut(s) 139, 1201
BpmI CTGGAG 2 cut(s) 788, 1353
Bpu10I CCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 694, 843
BsaHI GRCGYC 2 cut(s) 836, 1031
BsaI GGTCTC 3 cut(s) 54, 218, 1262
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 2 cut(s) 209, 396
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 4 cut(s) 50, 796, 1370, 1455
Bse21I CCTNAGG 1 cut(s) 1541
Bse3DI GCAATG 1 cut(s) 1284
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 3 cut(s) 24, 625, 759
BseLI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 5 cut(s) 349, 365, 487, 686, 1124
BseNI ACTGG 4 cut(s) 50, 796, 1370, 1455
BseRI GAGGAG 5 cut(s) 44, 974, 1164, 1288, 1555
BseSI GKGCMC 2 cut(s) 832, 1519
BseXI GCAGC 8 cut(s) 175, 268, 403, 447, 466, 469, 711, 1261
BsgI GTGCAG 2 cut(s) 473, 1394
Bsh1236I CGCG 3 cut(s) 131, 242, 247
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 383, 595, 947, 1049
BshNI GGYRCC 1 cut(s) 829
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 4 cut(s) 210, 217, 397, 833
BslFI GGGAC 1 cut(s) 1018
BslI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
BsmAI GTCTC 4 cut(s) 54, 218, 670, 1262
BsmFI GGGAC 1 cut(s) 1018
BsnI GGCC 4 cut(s) 383, 595, 947, 1049
Bso31I GGTCTC 3 cut(s) 54, 218, 1262
Bsp1286I GDGCHC 3 cut(s) 96, 832, 1519
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 221, 237, 871, 1504
Bsp68I TCGCGA 1 cut(s) 247
BspACI CCGC 4 cut(s) 109, 844, 1272, 1338
BspANI GGCC 4 cut(s) 383, 595, 947, 1049
BspCNI CTCAG 5 cut(s) 348, 366, 486, 687, 1123
BspFNI CGCG 3 cut(s) 131, 242, 247
BspLI GGNNCC 4 cut(s) 777, 831, 1182, 1321
BspPI GGATC 2 cut(s) 866, 1499
BspT107I GGYRCC 1 cut(s) 829
BspTNI GGTCTC 3 cut(s) 54, 218, 1262
BsrDI GCAATG 1 cut(s) 1284
BsrFI RCCGGY 1 cut(s) 832
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 4 cut(s) 50, 796, 1370, 1455
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 4 cut(s) 221, 237, 871, 1504
BssNI GRCGYC 2 cut(s) 836, 1031
BssSI CACGAG 3 cut(s) 119, 200, 1077
Bst2BI CACGAG 3 cut(s) 119, 200, 1077
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 6 cut(s) 608, 623, 914, 952, 1128, 1220
BstACI GRCGYC 2 cut(s) 836, 1031
BstAPI GCANNNNNTGC 1 cut(s) 460
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 6, 295, 428, 1377
BstDEI CTNAG 6 cut(s) 335, 374, 473, 695, 1110, 1541
BstENI CCTNNNNNAGG 2 cut(s) 305, 1540
BstF5I GGATG 3 cut(s) 24, 625, 759
BstFNI CGCG 3 cut(s) 131, 242, 247
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 224, 240, 874, 1507
BstMAI GTCTC 4 cut(s) 54, 218, 670, 1262
BstMBI GATC 4 cut(s) 221, 237, 871, 1504
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 2 cut(s) 364, 647
BstSCI CCNGG 1 cut(s) 507
BstSLI GKGCMC 2 cut(s) 832, 1519
BstUI CGCG 3 cut(s) 131, 242, 247
BstV1I GCAGC 8 cut(s) 175, 268, 403, 447, 466, 469, 711, 1261
BstV2I GAAGAC 2 cut(s) 139, 1201
BstXI CCANNNNNNTGG 1 cut(s) 803
Bsu36I CCTNAGG 1 cut(s) 1541
BsuRI GGCC 4 cut(s) 383, 595, 947, 1049
BtsCI GGATG 3 cut(s) 24, 625, 759
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 3 cut(s) 767, 1234, 1377
BtuMI TCGCGA 1 cut(s) 247
Cac8I GCNNGC 4 cut(s) 6, 295, 428, 1377
CaiI CAGNNNCTG 5 cut(s) 373, 460, 766, 1010, 1409
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 4 cut(s) 282, 370, 501, 669
CseI GACGC 3 cut(s) 137, 231, 825
Csp6I GTAC 8 cut(s) 124, 157, 274, 313, 346, 1018, 1256, 1561
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 4 cut(s) 277, 361, 644, 1420
CviQI GTAC 8 cut(s) 124, 157, 274, 313, 346, 1018, 1256, 1561
DdeI CTNAG 6 cut(s) 335, 374, 473, 695, 1110, 1541
DpnI GATC 4 cut(s) 223, 239, 873, 1506
DpnII GATC 4 cut(s) 221, 237, 871, 1504
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 2 cut(s) 381, 945
Ecl136II GAGCTC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 3 cut(s) 54, 218, 1262
Eco47I GGWCC 4 cut(s) 282, 370, 501, 669
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 743, 987
Eco81I CCTNAGG 1 cut(s) 1541
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 2 cut(s) 305, 1540
EcoO109I RGGNCCY 3 cut(s) 370, 501, 669
EcoRII CCWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 4 cut(s) 280, 364, 647, 1423
FalI AAGNNNNNCTT 6 cut(s) 488, 520, 656, 688, 890, 922
FaqI GGGAC 1 cut(s) 1018
FatI CATG 4 cut(s) 276, 360, 643, 1419
FblI GTMKAC 1 cut(s) 1478
Fnu4HI GCNGC 9 cut(s) 164, 257, 392, 455, 458, 461, 700, 844, 1275
FokI GGATG 3 cut(s) 11, 612, 766
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 9 cut(s) 164, 257, 392, 455, 458, 461, 700, 844, 1275
FspBI CTAG 5 cut(s) 617, 780, 801, 935, 1293
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 1 cut(s) 255
GluI GCNGC 9 cut(s) 164, 257, 392, 455, 458, 461, 700, 844, 1275
GsuI CTGGAG 2 cut(s) 788, 1353
HaeIII GGCC 4 cut(s) 383, 595, 947, 1049
HapII CCGG 4 cut(s) 210, 217, 397, 833
HgaI GACGC 3 cut(s) 137, 231, 825
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 2 cut(s) 836, 1031
Hin1II CATG 4 cut(s) 280, 364, 647, 1423
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 2 cut(s) 1233, 1445
HinfI GANTC 7 cut(s) 206, 331, 436, 477, 541, 632, 1548
HpaII CCGG 4 cut(s) 210, 217, 397, 833
HphI GGTGA 2 cut(s) 550, 1498
Hpy166II GTNNAC 6 cut(s) 157, 159, 1082, 1124, 1388, 1479
Hpy8I GTNNAC 6 cut(s) 157, 159, 1082, 1124, 1388, 1479
Hpy99I CGWCG 3 cut(s) 131, 247, 1033
HpyAV CCTTC 4 cut(s) 514, 1047, 1083, 1546
HpyCH4III ACNGT 6 cut(s) 608, 623, 914, 952, 1128, 1220
HpyCH4IV ACGT 1 cut(s) 1031
HpyCH4V TGCA 6 cut(s) 4, 454, 495, 662, 1277, 1375
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
HpyF3I CTNAG 6 cut(s) 335, 374, 473, 695, 1110, 1541
HpySE526I ACGT 1 cut(s) 1031
Hsp92I GRCGYC 2 cut(s) 836, 1031
Hsp92II CATG 4 cut(s) 280, 364, 647, 1423
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 4 cut(s) 221, 237, 871, 1504
LmnI GCTCC 6 cut(s) 99, 399, 961, 1287, 1301, 1372
Lsp1109I GCAGC 8 cut(s) 175, 268, 403, 447, 466, 469, 711, 1261
LweI GCATC 3 cut(s) 676, 744, 1015
MaeI CTAG 5 cut(s) 617, 780, 801, 935, 1293
MaeII ACGT 1 cut(s) 1031
MaeIII GTNAC 5 cut(s) 922, 1172, 1220, 1486, 1529
MalI GATC 4 cut(s) 223, 239, 873, 1506
MboI GATC 4 cut(s) 221, 237, 871, 1504
MboII GAAGA 3 cut(s) 139, 1206, 1281
MhlI GDGCHC 3 cut(s) 96, 832, 1519
MlsI TGGCCA 2 cut(s) 383, 947
MluCI AATT 5 cut(s) 74, 173, 249, 850, 1521
MluI ACGCGT 1 cut(s) 129
MluNI TGGCCA 2 cut(s) 383, 947
MlyI GAGTC 3 cut(s) 215, 486, 1542
Mox20I TGGCCA 2 cut(s) 383, 947
MscI TGGCCA 2 cut(s) 383, 947
MseI TTAA 5 cut(s) 416, 522, 849, 1463, 1573
MslI CAYNNNNRTG 2 cut(s) 711, 1418
Msp20I TGGCCA 2 cut(s) 383, 947
MspA1I CMGCKG 2 cut(s) 460, 1274
MspI CCGG 4 cut(s) 210, 217, 397, 833
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 3 cut(s) 131, 242, 247
MwoI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
NdeII GATC 4 cut(s) 221, 237, 871, 1504
NlaIII CATG 4 cut(s) 280, 364, 647, 1423
NlaIV GGNNCC 4 cut(s) 777, 831, 1182, 1321
NmuCI GTSAC 4 cut(s) 1172, 1220, 1486, 1529
NruI TCGCGA 1 cut(s) 247
NsbI TGCGCA 1 cut(s) 255
NspI RCATGY 2 cut(s) 364, 647
PceI AGGCCT 1 cut(s) 595
PciI ACATGT 1 cut(s) 643
PfeI GAWTC 4 cut(s) 331, 436, 541, 632
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 9 cut(s) 165, 258, 393, 456, 459, 462, 701, 845, 1276
Ple19I CGATCG 1 cut(s) 224
PleI GAGTC 3 cut(s) 214, 485, 1542
PpsI GAGTC 3 cut(s) 214, 485, 1542
PpuMI RGGWCCY 3 cut(s) 370, 501, 669
PscI ACATGT 1 cut(s) 643
PshBI ATTAAT 1 cut(s) 849
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 3 cut(s) 370, 501, 669
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 4 cut(s) 777, 831, 1182, 1321
PspPI GGNCC 4 cut(s) 282, 370, 501, 669
PspPPI RGGWCCY 3 cut(s) 370, 501, 669
PsrI GAACNNNNNNTAC 2 cut(s) 1390, 1422
PstNI CAGNNNCTG 5 cut(s) 373, 460, 766, 1010, 1409
PvuI CGATCG 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 460
RruI TCGCGA 1 cut(s) 247
RsaI GTAC 8 cut(s) 125, 158, 275, 314, 347, 1019, 1257, 1562
RsaNI GTAC 8 cut(s) 124, 157, 274, 313, 346, 1018, 1256, 1561
RseI CAYNNNNRTG 2 cut(s) 711, 1418
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 5 cut(s) 416, 522, 849, 1463, 1573
SatI GCNGC 9 cut(s) 164, 257, 392, 455, 458, 461, 700, 844, 1275
Sau3AI GATC 4 cut(s) 221, 237, 871, 1504
Sau96I GGNCC 4 cut(s) 282, 370, 501, 669
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 3 cut(s) 215, 486, 1542
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 3 cut(s) 96, 832, 1519
SfaNI GCATC 3 cut(s) 676, 744, 1015
SgrAI CRCCGGYG 1 cut(s) 832
SinI GGWCC 4 cut(s) 282, 370, 501, 669
SmiMI CAYNNNNRTG 2 cut(s) 711, 1418
SmlI CTYRAG 2 cut(s) 673, 858
SmoI CTYRAG 2 cut(s) 673, 858
Sse9I AATT 5 cut(s) 74, 173, 249, 850, 1521
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 4 cut(s) 109, 844, 1272, 1338
SspMI CTAG 5 cut(s) 617, 780, 801, 935, 1293
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 6 cut(s) 608, 623, 914, 952, 1128, 1220
TaiI ACGT 1 cut(s) 1034
TaqI TCGA 1 cut(s) 407
TasI AATT 5 cut(s) 74, 173, 249, 850, 1521
TatI WGTACW 5 cut(s) 156, 273, 345, 1017, 1255
TauI GCSGC 1 cut(s) 846
TfiI GAWTC 4 cut(s) 331, 436, 541, 632
Tru1I TTAA 5 cut(s) 416, 522, 849, 1463, 1573
Tru9I TTAA 5 cut(s) 416, 522, 849, 1463, 1573
TscAI CASTG 3 cut(s) 767, 1234, 1377
TseFI GTSAC 4 cut(s) 1172, 1220, 1486, 1529
TseI GCWGC 8 cut(s) 163, 256, 391, 454, 457, 460, 699, 1274
Tsp45I GTSAC 4 cut(s) 1172, 1220, 1486, 1529
TspDTI ATGAA 1 cut(s) 599
TspGWI ACGGA 3 cut(s) 906, 1161, 1266
TspRI CASTG 3 cut(s) 767, 1234, 1377
VpaK11BI GGWCC 4 cut(s) 282, 370, 501, 669
VspI ATTAAT 1 cut(s) 849
XagI CCTNNNNNAGG 2 cut(s) 305, 1540
XapI RAATTY 3 cut(s) 74, 173, 1521
XbaI TCTAGA 1 cut(s) 616
XceI RCATGY 2 cut(s) 364, 647
XmiI GTMKAC 1 cut(s) 1478
XspI CTAG 5 cut(s) 617, 780, 801, 935, 1293
ZraI GACGTC 1 cut(s) 1032
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.