MD02G1239100.v1.1

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007197
Physical Location & Seq
Reverse (-)
28674155 .. 28684056
9902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1239100.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGCAAGCACAGCAAAACCCTAACATCCCAGTTAGCGAAATCTACTGGTCTCTCCTCCACAAAGCCGACAAGAAATTCTCCAAAATCAGAGAGCTCCCCTATTACCACCGCAACAGGCACGAGTACGACGCGTATTTCTACAAGGTCTTCAAGGTGTACACGCAGCTATGGAAATTTCAGCAAGAAAATCGCCAGAAACTCGTGGAGTCCGGTCTCCGGCGATCGGAGATTGGCGAGATCGCCTCGCGAATTGCGCAGCTCTACTTGGGGCAGTACATGAGGATCAGCGAGGCAAGCTACCTATCCGAGGCGTACATATTCTACGAGGCGATTCTCAGCAGAGAATACTTCAAGGAGGGCATGTTTCAGGACCTGAGCTTGGCCAACAAGCAGCTCCGGTTTGTTTCGAGGTTTTTAATGGTGTGCTTGCTCTTGAATCGGCGAGAGATGGTGCAGCAGCTGCTCAATCAGCTCAGAGTCTTGGTTGATGAGTGCAAGAGGACCTTCCAGGAAACTGATTTTAAAGAATGGAAGCTGGTGATTCAGGAAATAGTTAGATTTTTGAAAGCTGACACAGCTTTTATGAATATCAGGCCTTTGAGATACAGTATTGTTCTAGACTGTCATCCTGATTCCTTACCACATGTTTCCACAGCAGTTGCAAAGAGGTCCTTGAGACTACGAGATGCTATACTGAGCAGCTACCATCATAATGAGGTAAAGTTTTCAGAGCTTACTCTTGACACTTTTAGGATGCTTCAGTGTCTGGAGTGGGAACCTAGTGGCTCATTTTACCAGTCTAGTGGGGCTAAAAATGGTCAGAATGGGGCACCAGCGTCCAGCCGCATTAATTACTCTCAAGATATTGCCGATCCTACTCTGCCACCTAATCCAAGAAAGGCTGTCTTATACCGTCCGTCTGTTACACATTTTCTAGCAGTTTTGGCCACAGTTTGTGAGGAGCTTCCCCCTGATGGAGTTATTTTGATATATCTGTCAGCTTCAGGTGCTGGAATGTACTCTGATGCGACGTCCCCTTCTACCTTGGCCTATTCGTCTGATAGGATAAACCCTTCTCGTGGACAAGGTAAAGGAGATGGTTCAAGTTCTCAGGTGGGCTGTTTACAGTTTGGTAGCCATAGAAACGGAGGAGTGAATAACATTTATCCGAGTGACTTGGTTCCATTTACAAGAAGACCGCTTGTTTTAGTTATTGACTGTGACAGCAGTGAAGCTTTTGAGGCTATAAACGGAGTACAAAAAGGAGAGACAGTTGCAATGCTCCTATCTTCTAGCTCCTCATATCACACTACTCTTGGTTCCTCTCGTCAGTCAAGCGGAAGCCTATTCACTATTTTCCTCACAGCTCCAGTGCAGGCTTTCTGTTTACTGCTTGGTATTTCAGGTTCTGACATTGACATGGAAACATTTGACAAGGCCGAGAAGCTTCTTTCCAGTTCGTTAAGTGACTGGGGGTCTACTTTGGTGACATTGGACACTATTGATCCTGTTTGGGCGCAGATTTTGAGTGACCCCTTCCTAAGGCGACTCCTTTTGAGGTTCATATTTTGTCAGGCTGTGCTATCACTCTTTGCTCCAACCTTCAATAAGAAGGAATTTATTCCAAAGTGTACGCCATGTCTTCCAGTTTCTGTCTCGCCAATGTCAACAGCTTGTCAAACTGTAATTTTACAGACAGCGAACATCTTTGGAGCCACCAGCAAGTTCATCTTCTCAGAAGGACTTGAACTCCCCGAAAATAGACAGGGCGATACAGAATCAATGTCATGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

599

Amino Acids

67.41

Weight (kDa)

7.51

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 18 - 533 3.7e-194 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1034
Acc16I TGCGCA 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 829
AccI GTMKAC 1 cut(s) 1478
AccII CGCG 2 cut(s) 131, 247
AciI CCGC 4 cut(s) 109, 844, 1199, 1338
AclWI GGATC 3 cut(s) 290, 866, 1499
AcoI YGGCCR 2 cut(s) 381, 945
AcsI RAATTY 3 cut(s) 74, 173, 1616
AcuI CTGAAG 2 cut(s) 743, 987
AcyI GRCGYC 1 cut(s) 1031
AfaI GTAC 7 cut(s) 125, 158, 275, 314, 1019, 1257, 1633
AfiI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
AflIII ACRYGT 2 cut(s) 129, 643
AgsI TTSAA 7 cut(s) 151, 352, 436, 565, 1104, 1606, 1748
AhdI GACNNNNNGTC 1 cut(s) 1474
AjnI CCWGG 1 cut(s) 507
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 5 cut(s) 54, 218, 670, 1262, 1660
AlwI GGATC 3 cut(s) 290, 866, 1499
AlwNI CAGNNNCTG 6 cut(s) 373, 460, 766, 1010, 1409, 1652
AoxI GGCC 5 cut(s) 381, 593, 945, 1047, 1437
ApeKI GCWGC 7 cut(s) 163, 256, 391, 454, 457, 460, 699
ApoI RAATTY 3 cut(s) 74, 173, 1616
AseI ATTAAT 1 cut(s) 849
Asp700I GAANNNNTTC 1 cut(s) 1620
AspLEI GCGC 2 cut(s) 256, 1519
AspS9I GGNCC 3 cut(s) 370, 501, 669
AsuHPI GGTGA 2 cut(s) 550, 1498
AvaII GGWCC 3 cut(s) 370, 501, 669
AxyI CCTNAGG 1 cut(s) 1541
BaeGI GKGCMC 1 cut(s) 832
BalI TGGCCA 2 cut(s) 383, 947
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 3 cut(s) 119, 200, 1077
BbsI GAAGAC 3 cut(s) 139, 1201, 1634
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 7 cut(s) 175, 268, 403, 447, 466, 469, 711
BccI CCATC 4 cut(s) 442, 714, 968, 1091
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 5 cut(s) 54, 218, 670, 1262, 1660
BfaI CTAG 5 cut(s) 617, 780, 801, 935, 1293
BisI GCNGC 8 cut(s) 164, 257, 392, 455, 458, 461, 700, 844
BlsI GCNGC 8 cut(s) 165, 258, 393, 456, 459, 462, 701, 845
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 3 cut(s) 370, 501, 669
BmeRI GACNNNNNGTC 1 cut(s) 1474
BmgT120I GGNCC 3 cut(s) 370, 501, 669
BmiI GGNNCC 5 cut(s) 777, 831, 1182, 1321, 1714
BmrFI CCNGG 1 cut(s) 509
BmrI ACTGGG 2 cut(s) 23, 1480
BmsI GCATC 3 cut(s) 676, 744, 1015
BmuI ACTGGG 2 cut(s) 23, 1480
BpiI GAAGAC 3 cut(s) 139, 1201, 1634
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 2 cut(s) 788, 1353
Bpu10I CCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 694, 843
BsaHI GRCGYC 1 cut(s) 1031
BsaI GGTCTC 2 cut(s) 54, 218
BsaJI CCNNGG 2 cut(s) 306, 1044
BsaWI WCCGGW 2 cut(s) 209, 396
BsaXI ACNNNNNCTCC 6 cut(s) 347, 377, 1257, 1287, 1734, 1764
Bsc4I CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
Bse1I ACTGG 7 cut(s) 29, 50, 796, 1370, 1455, 1475, 1646
Bse21I CCTNAGG 1 cut(s) 1541
Bse3DI GCAATG 1 cut(s) 1284
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 306, 1044
BseGI GGATG 3 cut(s) 24, 625, 759
BseLI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 6 cut(s) 349, 365, 487, 686, 1124, 1749
BseNI ACTGG 7 cut(s) 29, 50, 796, 1370, 1455, 1475, 1646
BseRI GAGGAG 4 cut(s) 44, 974, 1164, 1288
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 7 cut(s) 175, 268, 403, 447, 466, 469, 711
BsgI GTGCAG 2 cut(s) 473, 1394
Bsh1236I CGCG 2 cut(s) 131, 247
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 5 cut(s) 383, 595, 947, 1049, 1439
BshNI GGYRCC 1 cut(s) 829
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 3 cut(s) 210, 217, 397
BslFI GGGAC 1 cut(s) 1018
BslI CCNNNNNNNGG 7 cut(s) 216, 223, 307, 379, 974, 1079, 1542
BsmAI GTCTC 5 cut(s) 54, 218, 670, 1262, 1660
BsmFI GGGAC 1 cut(s) 1018
BsnI GGCC 5 cut(s) 383, 595, 947, 1049, 1439
Bso31I GGTCTC 2 cut(s) 54, 218
Bsp1286I GDGCHC 2 cut(s) 96, 832
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 5 cut(s) 221, 237, 282, 871, 1504
Bsp68I TCGCGA 1 cut(s) 247
BspACI CCGC 4 cut(s) 109, 844, 1199, 1338
BspANI GGCC 5 cut(s) 383, 595, 947, 1049, 1439
BspCNI CTCAG 6 cut(s) 348, 366, 486, 687, 1123, 1748
BspFNI CGCG 2 cut(s) 131, 247
BspHI TCATGA 1 cut(s) 1793
BspLI GGNNCC 5 cut(s) 777, 831, 1182, 1321, 1714
BspPI GGATC 3 cut(s) 290, 866, 1499
BspT107I GGYRCC 1 cut(s) 829
BspTNI GGTCTC 2 cut(s) 54, 218
BsrDI GCAATG 1 cut(s) 1284
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 7 cut(s) 29, 50, 796, 1370, 1455, 1475, 1646
BssECI CCNNGG 2 cut(s) 306, 1044
BssMI GATC 5 cut(s) 221, 237, 282, 871, 1504
BssNI GRCGYC 1 cut(s) 1031
BssSI CACGAG 3 cut(s) 119, 200, 1077
BssT1I CCWWGG 1 cut(s) 1044
Bst2BI CACGAG 3 cut(s) 119, 200, 1077
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 8 cut(s) 608, 623, 914, 952, 1128, 1220, 1273, 1684
BstACI GRCGYC 1 cut(s) 1031
BstAPI GCANNNNNTGC 1 cut(s) 460
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 6, 295, 428, 1377
BstDEI CTNAG 7 cut(s) 335, 374, 473, 695, 1110, 1541, 1735
BstENI CCTNNNNNAGG 2 cut(s) 305, 1540
BstF5I GGATG 3 cut(s) 24, 625, 759
BstFNI CGCG 2 cut(s) 131, 247
BstHHI GCGC 2 cut(s) 256, 1519
BstKTI GATC 5 cut(s) 224, 240, 285, 874, 1507
BstMAI GTCTC 5 cut(s) 54, 218, 670, 1262, 1660
BstMBI GATC 5 cut(s) 221, 237, 282, 871, 1504
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 2 cut(s) 364, 647
BstSCI CCNGG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 2 cut(s) 131, 247
BstV1I GCAGC 7 cut(s) 175, 268, 403, 447, 466, 469, 711
BstV2I GAAGAC 3 cut(s) 139, 1201, 1634
BstXI CCANNNNNNTGG 1 cut(s) 803
Bsu36I CCTNAGG 1 cut(s) 1541
BsuRI GGCC 5 cut(s) 383, 595, 947, 1049, 1439
BtsCI GGATG 3 cut(s) 24, 625, 759
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 3 cut(s) 767, 1234, 1377
BtuMI TCGCGA 1 cut(s) 247
Cac8I GCNNGC 4 cut(s) 6, 295, 428, 1377
CaiI CAGNNNCTG 6 cut(s) 373, 460, 766, 1010, 1409, 1652
CciI TCATGA 1 cut(s) 1793
CfoI GCGC 2 cut(s) 256, 1519
Cfr13I GGNCC 3 cut(s) 370, 501, 669
CseI GACGC 2 cut(s) 137, 825
Csp6I GTAC 7 cut(s) 124, 157, 274, 313, 1018, 1256, 1632
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 7 cut(s) 277, 361, 644, 1420, 1638, 1788, 1794
CviQI GTAC 7 cut(s) 124, 157, 274, 313, 1018, 1256, 1632
DdeI CTNAG 7 cut(s) 335, 374, 473, 695, 1110, 1541, 1735
DpnI GATC 5 cut(s) 223, 239, 284, 873, 1506
DpnII GATC 5 cut(s) 221, 237, 282, 871, 1504
DraI TTTAAA 1 cut(s) 523
DriI GACNNNNNGTC 1 cut(s) 1474
EaeI YGGCCR 2 cut(s) 381, 945
Eam1105I GACNNNNNGTC 1 cut(s) 1474
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 1044
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 2 cut(s) 54, 218
Eco47I GGWCC 3 cut(s) 370, 501, 669
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 743, 987
Eco81I CCTNAGG 1 cut(s) 1541
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 2 cut(s) 305, 1540
EcoO109I RGGNCCY 3 cut(s) 370, 501, 669
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 1044
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 7 cut(s) 280, 364, 647, 1423, 1641, 1791, 1797
FalI AAGNNNNNCTT 8 cut(s) 488, 520, 656, 688, 890, 922, 1715, 1747
FaqI GGGAC 1 cut(s) 1018
FatI CATG 7 cut(s) 276, 360, 643, 1419, 1637, 1787, 1793
FblI GTMKAC 1 cut(s) 1478
Fnu4HI GCNGC 8 cut(s) 164, 257, 392, 455, 458, 461, 700, 844
FokI GGATG 3 cut(s) 11, 612, 766
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 8 cut(s) 164, 257, 392, 455, 458, 461, 700, 844
FspBI CTAG 5 cut(s) 617, 780, 801, 935, 1293
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 2 cut(s) 255, 1518
GluI GCNGC 8 cut(s) 164, 257, 392, 455, 458, 461, 700, 844
GsuI CTGGAG 2 cut(s) 788, 1353
HaeIII GGCC 5 cut(s) 383, 595, 947, 1049, 1439
HapII CCGG 3 cut(s) 210, 217, 397
HgaI GACGC 2 cut(s) 137, 825
HhaI GCGC 2 cut(s) 256, 1519
Hin1I GRCGYC 1 cut(s) 1031
Hin1II CATG 7 cut(s) 280, 364, 647, 1423, 1641, 1791, 1797
Hin6I GCGC 2 cut(s) 254, 1517
HinP1I GCGC 2 cut(s) 254, 1517
HincII GTYRAC 1 cut(s) 1668
HindII GTYRAC 1 cut(s) 1668
HindIII AAGCTT 2 cut(s) 1233, 1445
HinfI GANTC 8 cut(s) 206, 331, 436, 477, 541, 632, 1548, 1778
HpaII CCGG 3 cut(s) 210, 217, 397
HphI GGTGA 2 cut(s) 550, 1498
Hpy166II GTNNAC 8 cut(s) 157, 159, 1082, 1124, 1388, 1479, 1632, 1668
Hpy8I GTNNAC 8 cut(s) 157, 159, 1082, 1124, 1388, 1479, 1632, 1668
Hpy99I CGWCG 2 cut(s) 131, 1033
HpyAV CCTTC 7 cut(s) 514, 1047, 1083, 1546, 1606, 1612, 1733
HpyCH4III ACNGT 8 cut(s) 608, 623, 914, 952, 1128, 1220, 1273, 1684
HpyCH4IV ACGT 1 cut(s) 1031
HpyCH4V TGCA 6 cut(s) 4, 454, 495, 662, 1277, 1375
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
HpyF3I CTNAG 7 cut(s) 335, 374, 473, 695, 1110, 1541, 1735
HpySE526I ACGT 1 cut(s) 1031
Hsp92I GRCGYC 1 cut(s) 1031
Hsp92II CATG 7 cut(s) 280, 364, 647, 1423, 1641, 1791, 1797
HspAI GCGC 2 cut(s) 254, 1517
Kzo9I GATC 5 cut(s) 221, 237, 282, 871, 1504
LmnI GCTCC 8 cut(s) 99, 399, 961, 1287, 1301, 1372, 1600, 1712
Lsp1109I GCAGC 7 cut(s) 175, 268, 403, 447, 466, 469, 711
LweI GCATC 3 cut(s) 676, 744, 1015
MaeI CTAG 5 cut(s) 617, 780, 801, 935, 1293
MaeII ACGT 1 cut(s) 1031
MaeIII GTNAC 6 cut(s) 922, 1172, 1220, 1466, 1486, 1529
MalI GATC 5 cut(s) 223, 239, 284, 873, 1506
MboI GATC 5 cut(s) 221, 237, 282, 871, 1504
MboII GAAGA 5 cut(s) 139, 1206, 1281, 1634, 1723
MhlI GDGCHC 2 cut(s) 96, 832
MlsI TGGCCA 2 cut(s) 383, 947
MluCI AATT 6 cut(s) 74, 173, 249, 850, 1616, 1686
MluI ACGCGT 1 cut(s) 129
MluNI TGGCCA 2 cut(s) 383, 947
MlyI GAGTC 3 cut(s) 215, 486, 1542
MmeI TCCRAC 1 cut(s) 1622
Mox20I TGGCCA 2 cut(s) 383, 947
MroXI GAANNNNTTC 1 cut(s) 1620
MscI TGGCCA 2 cut(s) 383, 947
MseI TTAA 4 cut(s) 416, 522, 849, 1463
MslI CAYNNNNRTG 3 cut(s) 711, 1418, 1792
Msp20I TGGCCA 2 cut(s) 383, 947
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 3 cut(s) 210, 217, 397
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 2 cut(s) 131, 247
MwoI GCNNNNNNNGC 9 cut(s) 10, 253, 294, 460, 469, 575, 944, 1007, 1241
NdeII GATC 5 cut(s) 221, 237, 282, 871, 1504
NlaIII CATG 7 cut(s) 280, 364, 647, 1423, 1641, 1791, 1797
NlaIV GGNNCC 5 cut(s) 777, 831, 1182, 1321, 1714
NmeAIII GCCGAG 1 cut(s) 1465
NmuCI GTSAC 5 cut(s) 1172, 1220, 1466, 1486, 1529
NruI TCGCGA 1 cut(s) 247
NsbI TGCGCA 1 cut(s) 255
NspI RCATGY 2 cut(s) 364, 647
PagI TCATGA 1 cut(s) 1793
PceI AGGCCT 1 cut(s) 595
PciI ACATGT 1 cut(s) 643
PdmI GAANNNNTTC 1 cut(s) 1620
PfeI GAWTC 5 cut(s) 331, 436, 541, 632, 1778
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 8 cut(s) 165, 258, 393, 456, 459, 462, 701, 845
Ple19I CGATCG 1 cut(s) 224
PleI GAGTC 3 cut(s) 214, 485, 1542
PpsI GAGTC 3 cut(s) 214, 485, 1542
PpuMI RGGWCCY 3 cut(s) 370, 501, 669
PscI ACATGT 1 cut(s) 643
PshBI ATTAAT 1 cut(s) 849
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 3 cut(s) 370, 501, 669
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 5 cut(s) 777, 831, 1182, 1321, 1714
PspPI GGNCC 3 cut(s) 370, 501, 669
PspPPI RGGWCCY 3 cut(s) 370, 501, 669
PsrI GAACNNNNNNTAC 2 cut(s) 1390, 1422
PstNI CAGNNNCTG 6 cut(s) 373, 460, 766, 1010, 1409, 1652
PvuI CGATCG 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 460
RruI TCGCGA 1 cut(s) 247
RsaI GTAC 7 cut(s) 125, 158, 275, 314, 1019, 1257, 1633
RsaNI GTAC 7 cut(s) 124, 157, 274, 313, 1018, 1256, 1632
RseI CAYNNNNRTG 3 cut(s) 711, 1418, 1792
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 4 cut(s) 416, 522, 849, 1463
SatI GCNGC 8 cut(s) 164, 257, 392, 455, 458, 461, 700, 844
Sau3AI GATC 5 cut(s) 221, 237, 282, 871, 1504
Sau96I GGNCC 3 cut(s) 370, 501, 669
SchI GAGTC 3 cut(s) 215, 486, 1542
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 2 cut(s) 96, 832
SfaNI GCATC 3 cut(s) 676, 744, 1015
SinI GGWCC 3 cut(s) 370, 501, 669
SmiMI CAYNNNNRTG 3 cut(s) 711, 1418, 1792
SmlI CTYRAG 2 cut(s) 673, 858
SmoI CTYRAG 2 cut(s) 673, 858
Sse9I AATT 6 cut(s) 74, 173, 249, 850, 1616, 1686
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 4 cut(s) 109, 844, 1199, 1338
SspMI CTAG 5 cut(s) 617, 780, 801, 935, 1293
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 8 cut(s) 608, 623, 914, 952, 1128, 1220, 1273, 1684
TaiI ACGT 1 cut(s) 1034
TaqI TCGA 1 cut(s) 407
TasI AATT 6 cut(s) 74, 173, 249, 850, 1616, 1686
TatI WGTACW 4 cut(s) 156, 273, 1017, 1255
TauI GCSGC 1 cut(s) 846
TfiI GAWTC 5 cut(s) 331, 436, 541, 632, 1778
Tru1I TTAA 4 cut(s) 416, 522, 849, 1463
Tru9I TTAA 4 cut(s) 416, 522, 849, 1463
TscAI CASTG 3 cut(s) 767, 1234, 1377
TseFI GTSAC 5 cut(s) 1172, 1220, 1466, 1486, 1529
TseI GCWGC 7 cut(s) 163, 256, 391, 454, 457, 460, 699
Tsp45I GTSAC 5 cut(s) 1172, 1220, 1466, 1486, 1529
TspDTI ATGAA 4 cut(s) 599, 1552, 1717, 1782
TspGWI ACGGA 3 cut(s) 906, 1161, 1266
TspRI CASTG 3 cut(s) 767, 1234, 1377
VpaK11BI GGWCC 3 cut(s) 370, 501, 669
VspI ATTAAT 1 cut(s) 849
XagI CCTNNNNNAGG 2 cut(s) 305, 1540
XapI RAATTY 3 cut(s) 74, 173, 1616
XbaI TCTAGA 1 cut(s) 616
XceI RCATGY 2 cut(s) 364, 647
XmiI GTMKAC 1 cut(s) 1478
XmnI GAANNNNTTC 1 cut(s) 1620
XspI CTAG 5 cut(s) 617, 780, 801, 935, 1293
ZraI GACGTC 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.