AT3G05840
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005949
Physical Location & Seq
Forward (+)
1739777 .. 1743163
3387 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05840.1

Sequence Viewer

Length: 1230 bp
ATGGCCTCGGTGGGCATAGAGCCTAGTGCCGCGGTTAGAGAATCTACTGGAAACGTTACTGATGCTGATAGATTACCCGAGGAGATGAAGGACATGAAAATTCAAGATGATAAAGAAATGGAAGCTACGATTGTTAATGGCAATGTGACTGAGACTGGCCATATAATAGTAACTACTATAGGAGGAAGAAATGGCCAGCCAAAACAGACAATCAGTTACATGGCGGAGCGAGTTGTTGGACATGGCTCCTTTGGTGTTGTGTTTCAGGCCAAATGTTTAGAAACAGGAGAAACTGTTGCTATAAAGAAAGTTCTACAAGATCGGAGGTACAAGAATCGTGAGCTTCAAACAATGAGGCTACTTGACCATCCAAATGTTGTGTCTTTGAAACATTGTTTCTTCTCTACAACCGAAAAAGATGAGCTTTATCTCAACTTGGTTCTGGAATACGTTCCGGAAACTGTGCACCGCGTCATCAAACACTACAACAAACTTAACCAACGAATGCCTCTCGTTTACGTCAAACTTTACACTTATCAGATTTTTAGGTCCTTATCCTACATTCACCGATGTATCGGCGTATGTCATCGAGACATCAAACCTCAAAACTTGTTGGTAAATCCACACACTCATCAAGTGAAACTATGCGATTTTGGAAGTGCGAAAGTATTGGTTAAAGGAGAGCCAAACATTTCATACATTTGCTCGAGGTATTACAGAGCACCTGAGCTCATTTTTGGAGCCACCGAGTATACTACAGCCATTGATGTCTGGTCTGCAGGATGTGTTCTCGCCGAGCTTCTTCTCGGGCAGCCATTGTTCCCGGGTGAGAGCGGTGTTGATCAACTTGTAGAGATTATAAAGGTTTTGGGAACACCAACAAGGGAAGAAATCAAATGCATGAACCCGAATTACACAGAGTTCAAATTTCCTCAGATTAAAGCTCATCCATGGCATAAGATTTTCCACAAGAGAATGCCTCCAGAAGCTGTTGATTTGGTCTCAAGGCTTCTTCAATACTCTCCCAATCTCCGTTGTGCTGCTCTTGATTCATTGGTCCACCCATTCTTTGACGAGCTAAGAGATCCGAATGCGCGATTACCCAACGGACGTTTCCTTCCACCGCTCTTTAACTTTAAGCCTCATGAACTTAAAGGTGTGCCTGTGGAGATGGTGGCGAAGTTAGTTCCAGAACATGCGAGGAAGCAATGTCCGTGGCTCAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009933 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032502 GO:0036211 GO:0042538 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0047484 GO:0048507 GO:0048518 GO:0048532 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050896 GO:0065007 GO:0071704 GO:0080134 GO:0140096 GO:1901000 GO:1901002 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01521 ko04012 ko04062 ko04110 ko04150 ko04151 ko04310 ko04340 ko04341 ko04360 ko04390 ko04510 ko04550 ko04657 ko04660 ko04662 ko04711 ko04722 ko04728 ko04910 ko04916 ko04917 ko04919 ko04931 ko04932 ko04934 ko05010 ko05160 ko05162 ko05164 ko05165 ko05166 ko05167 ko05169 ko05200 ko05210 ko05213 ko05215 ko05217 ko05224 ko05225 ko05226 map01521 map04012 map04062 map04110 map04150 map04151 map04310 map04340 map04341 map04360 map04390 map04510 map04550 map04657 map04660 map04662 map04711 map04722 map04728 map04910 map04916 map04917 map04919 map04931 map04932 map04934 map05010 map05160 map05162 map05164 map05165 map05166 map05167 map05169 map05200 map05210 map05213 map05215 map05217 map05224 map05225 map05226
Pfam Domains
Protein Families
Pkinase

Protein Analysis

409

Amino Acids

46.59

Weight (kDa)

8.55

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 74 - 357 1.9e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 76 - 275 8.1e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
AccBSI CCGCTC 2 cut(s) 834, 1126
AccI GTMKAC 1 cut(s) 752
AccII CGCG 3 cut(s) 32, 471, 1096
AccIII TCCGGA 1 cut(s) 454
AciI CCGC 6 cut(s) 30, 32, 224, 469, 834, 1124
AclI AACGTT 1 cut(s) 54
AclWI GGATC 1 cut(s) 1079
AcoI YGGCCR 2 cut(s) 157, 193
AcsI RAATTY 2 cut(s) 99, 926
AfaI GTAC 1 cut(s) 329
AgsI TTSAA 5 cut(s) 104, 347, 388, 925, 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 1097, 1129
AluBI AGCT 8 cut(s) 125, 343, 424, 730, 799, 944, 989, 1078
AluI AGCT 8 cut(s) 125, 343, 424, 730, 799, 944, 989, 1078
Alw21I GWGCWC 3 cut(s) 468, 724, 732
Alw26I GTCTC 3 cut(s) 146, 585, 1006
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 1 cut(s) 1079
AlwNI CAGNNNCTG 1 cut(s) 989
Ama87I CYCGRG 4 cut(s) 77, 706, 806, 823
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 4 cut(s) 3, 157, 193, 267
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 2 cut(s) 811, 1040
ApoI RAATTY 2 cut(s) 99, 926
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 1096
AspS9I GGNCC 2 cut(s) 549, 1057
AsuC2I CCSGG 2 cut(s) 824, 825
AsuHPI GGTGA 2 cut(s) 557, 839
AvaI CYCGRG 4 cut(s) 77, 706, 806, 823
AvaII GGWCC 2 cut(s) 549, 1057
BaeGI GKGCMC 1 cut(s) 468
BaeI ACNNNNGTAYC 2 cut(s) 556, 589
BalI TGGCCA 2 cut(s) 159, 195
BanII GRGCYC 1 cut(s) 732
Bbv12I GWGCWC 3 cut(s) 468, 724, 732
BbvI GCAGC 2 cut(s) 823, 1027
BccI CCATC 2 cut(s) 375, 1165
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 824, 825
BcoDI GTCTC 3 cut(s) 146, 585, 1006
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 3 cut(s) 177, 756, 777
BisI GCNGC 3 cut(s) 30, 812, 1041
BlsI GCNGC 3 cut(s) 31, 813, 1042
Bme1390I CCNGG 2 cut(s) 824, 825
Bme18I GGWCC 2 cut(s) 549, 1057
BmeT110I CYCGRG 4 cut(s) 77, 706, 806, 823
BmgT120I GGNCC 2 cut(s) 549, 1057
BmiI GGNNCC 2 cut(s) 247, 742
BmrFI CCNGG 2 cut(s) 824, 825
BmsI GCATC 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 964, 996
BpmI CTGGAG 1 cut(s) 966
Bpu10I CCTNAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 988
BpuMI CCSGG 2 cut(s) 824, 825
BsaI GGTCTC 1 cut(s) 1006
BsaJI CCNNGG 6 cut(s) 6, 30, 78, 823, 950, 1214
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bse1I ACTGG 2 cut(s) 52, 160
Bse3DI GCAATG 2 cut(s) 148, 1214
BseAI TCCGGA 1 cut(s) 454
BseDI CCNNGG 6 cut(s) 6, 30, 78, 823, 950, 1214
BseGI GGATG 3 cut(s) 367, 788, 946
BseMI GCAATG 2 cut(s) 148, 1214
BseMII CTCAG 3 cut(s) 141, 717, 947
BseNI ACTGG 2 cut(s) 52, 160
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 2 cut(s) 823, 1027
Bsh1236I CGCG 3 cut(s) 32, 471, 1096
BshFI GGCC 4 cut(s) 5, 159, 195, 269
BsiHKAI GWGCWC 3 cut(s) 468, 724, 732
BsiHKCI CYCGRG 4 cut(s) 77, 706, 806, 823
BsiSI CCGG 2 cut(s) 455, 824
BsmAI GTCTC 3 cut(s) 146, 585, 1006
BsmI GAATGC 3 cut(s) 510, 981, 1096
BsnI GGCC 4 cut(s) 5, 159, 195, 269
Bso31I GGTCTC 1 cut(s) 1006
BsoBI CYCGRG 4 cut(s) 77, 706, 806, 823
Bsp1286I GDGCHC 3 cut(s) 468, 724, 732
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 3 cut(s) 319, 841, 1084
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 6 cut(s) 30, 32, 224, 469, 834, 1124
BspANI GGCC 4 cut(s) 5, 159, 195, 269
BspCNI CTCAG 3 cut(s) 142, 718, 946
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 3 cut(s) 32, 471, 1096
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 2 cut(s) 247, 742
BspMAI CTGCAG 1 cut(s) 781
BspPI GGATC 1 cut(s) 1079
BspTNI GGTCTC 1 cut(s) 1006
BsrBI CCGCTC 2 cut(s) 834, 1126
BsrDI GCAATG 2 cut(s) 148, 1214
BsrI ACTGG 2 cut(s) 52, 160
BssECI CCNNGG 6 cut(s) 6, 30, 78, 823, 950, 1214
BssMI GATC 3 cut(s) 319, 841, 1084
BssNAI GTATAC 1 cut(s) 753
BssT1I CCWWGG 1 cut(s) 950
Bst1107I GTATAC 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 295, 463
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 5 cut(s) 150, 726, 933, 1079, 1220
BstDSI CCRYGG 3 cut(s) 30, 950, 1214
BstF5I GGATG 3 cut(s) 367, 788, 946
BstFNI CGCG 3 cut(s) 32, 471, 1096
BstHHI GCGC 1 cut(s) 1096
BstKTI GATC 3 cut(s) 322, 844, 1087
BstMAI GTCTC 3 cut(s) 146, 585, 1006
BstMBI GATC 3 cut(s) 319, 841, 1084
BstNSI RCATGY 1 cut(s) 1199
BstSCI CCNGG 2 cut(s) 822, 823
BstSFI CTRYAG 3 cut(s) 177, 756, 777
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 3 cut(s) 32, 471, 1096
BstV1I GCAGC 2 cut(s) 823, 1027
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BstZ17I GTATAC 1 cut(s) 753
BsuRI GGCC 4 cut(s) 5, 159, 195, 269
BtgI CCRYGG 3 cut(s) 30, 950, 1214
BtsCI GGATG 3 cut(s) 367, 788, 946
Cac8I GCNNGC 1 cut(s) 197
CaiI CAGNNNCTG 1 cut(s) 989
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 1 cut(s) 1096
Cfr13I GGNCC 2 cut(s) 549, 1057
Cfr42I CCGCGG 1 cut(s) 33
Cfr9I CCCGGG 1 cut(s) 823
CseI GACGC 1 cut(s) 460
Csp6I GTAC 1 cut(s) 328
CspCI CAANNNNNGTGG 1 cut(s) 1196
CviAII CATG 7 cut(s) 94, 220, 242, 901, 951, 1145, 1196
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 5 cut(s) 150, 726, 933, 1079, 1220
DpnI GATC 3 cut(s) 321, 843, 1086
DpnII GATC 3 cut(s) 319, 841, 1084
EaeI YGGCCR 2 cut(s) 157, 193
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 950
Eco24I GRGCYC 1 cut(s) 732
Eco31I GGTCTC 1 cut(s) 1006
Eco47I GGWCC 2 cut(s) 549, 1057
Eco53kI GAGCTC 1 cut(s) 730
Eco88I CYCGRG 4 cut(s) 77, 706, 806, 823
EcoICRI GAGCTC 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 950
EcoT22I ATGCAT 1 cut(s) 902
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 950
FaeI CATG 7 cut(s) 97, 223, 245, 904, 954, 1148, 1199
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FatI CATG 7 cut(s) 93, 219, 241, 900, 950, 1144, 1195
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 3 cut(s) 30, 812, 1041
FokI GGATG 3 cut(s) 354, 795, 933
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 3 cut(s) 30, 812, 1041
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 1095
GluI GCNGC 3 cut(s) 30, 812, 1041
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 4 cut(s) 5, 159, 195, 269
HapII CCGG 2 cut(s) 455, 824
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 1096
Hin1II CATG 7 cut(s) 97, 223, 245, 904, 954, 1148, 1199
Hin6I GCGC 1 cut(s) 1094
HinP1I GCGC 1 cut(s) 1094
HinfI GANTC 3 cut(s) 41, 334, 1049
HpaII CCGG 2 cut(s) 455, 824
HphI GGTGA 2 cut(s) 557, 839
Hpy166II GTNNAC 4 cut(s) 466, 517, 753, 1060
Hpy188I TCNGA 4 cut(s) 324, 540, 936, 1089
Hpy188III TCNNGA 9 cut(s) 104, 338, 443, 455, 590, 983, 1046, 1145, 1190
Hpy8I GTNNAC 4 cut(s) 466, 517, 753, 1060
HpyAV CCTTC 2 cut(s) 82, 1127
HpyCH4III ACNGT 2 cut(s) 295, 463
HpyCH4IV ACGT 4 cut(s) 54, 450, 519, 1111
HpyCH4V TGCA 3 cut(s) 466, 779, 900
HpyF3I CTNAG 5 cut(s) 150, 726, 933, 1079, 1220
HpySE526I ACGT 4 cut(s) 54, 450, 519, 1111
Hsp92II CATG 7 cut(s) 97, 223, 245, 904, 954, 1148, 1199
HspAI GCGC 1 cut(s) 1094
Kpn2I TCCGGA 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 841
KspI CCGCGG 1 cut(s) 33
Kzo9I GATC 3 cut(s) 319, 841, 1084
LmnI GCTCC 3 cut(s) 226, 251, 740
Lsp1109I GCAGC 2 cut(s) 823, 1027
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 24
MaeII ACGT 4 cut(s) 54, 450, 519, 1111
MaeIII GTNAC 4 cut(s) 55, 145, 169, 215
MalI GATC 3 cut(s) 321, 843, 1086
MbiI CCGCTC 2 cut(s) 834, 1126
MboI GATC 3 cut(s) 319, 841, 1084
MboII GAAGA 5 cut(s) 198, 391, 794, 899, 1004
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 3 cut(s) 468, 724, 732
MlsI TGGCCA 2 cut(s) 159, 195
MluCI AATT 3 cut(s) 99, 910, 926
MluNI TGGCCA 2 cut(s) 159, 195
MmeI TCCRAC 1 cut(s) 217
Mox20I TGGCCA 2 cut(s) 159, 195
Mph1103I ATGCAT 1 cut(s) 902
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 450
MscI TGGCCA 2 cut(s) 159, 195
MseI TTAA 7 cut(s) 135, 495, 675, 939, 1131, 1137, 1152
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 2 cut(s) 159, 195
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 455, 824
MspR9I CCNGG 2 cut(s) 824, 825
Mva1269I GAATGC 3 cut(s) 510, 981, 1096
MvnI CGCG 3 cut(s) 32, 471, 1096
NciI CCSGG 2 cut(s) 824, 825
NcoI CCATGG 1 cut(s) 950
NdeII GATC 3 cut(s) 319, 841, 1084
NlaIII CATG 7 cut(s) 97, 223, 245, 904, 954, 1148, 1199
NlaIV GGNNCC 2 cut(s) 247, 742
NmeAIII GCCGAG 1 cut(s) 820
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 902
NspI RCATGY 1 cut(s) 1199
PaeR7I CTCGAG 1 cut(s) 706
PagI TCATGA 1 cut(s) 1144
PctI GAATGC 3 cut(s) 510, 981, 1096
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 3 cut(s) 41, 334, 1049
PkrI GCNGC 3 cut(s) 31, 813, 1042
PpuMI RGGWCCY 1 cut(s) 549
PsiI TTATAA 1 cut(s) 860
Psp124BI GAGCTC 1 cut(s) 732
Psp1406I AACGTT 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 549
PspN4I GGNNCC 2 cut(s) 247, 742
PspPI GGNCC 2 cut(s) 549, 1057
PspPPI RGGWCCY 1 cut(s) 549
PspXI VCTCGAGB 1 cut(s) 706
PsrI GAACNNNNNNTAC 2 cut(s) 896, 928
PstI CTGCAG 1 cut(s) 781
PstNI CAGNNNCTG 1 cut(s) 989
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 372
SacI GAGCTC 1 cut(s) 732
SacII CCGCGG 1 cut(s) 33
SaqAI TTAA 7 cut(s) 135, 495, 675, 939, 1131, 1137, 1152
SatI GCNGC 3 cut(s) 30, 812, 1041
Sau3AI GATC 3 cut(s) 319, 841, 1084
Sau96I GGNCC 2 cut(s) 549, 1057
ScrFI CCNGG 2 cut(s) 824, 825
SduI GDGCHC 3 cut(s) 468, 724, 732
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 3 cut(s) 177, 756, 777
Sfr274I CTCGAG 1 cut(s) 706
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 2 cut(s) 549, 1057
SlaI CTCGAG 1 cut(s) 706
SmaI CCCGGG 1 cut(s) 825
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 706, 1003
SmoI CTYRAG 2 cut(s) 706, 1003
Sse9I AATT 3 cut(s) 99, 910, 926
SsiI CCGC 6 cut(s) 30, 32, 224, 469, 834, 1124
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 822, 823
StyI CCWWGG 1 cut(s) 950
TaaI ACNGT 2 cut(s) 295, 463
TaiI ACGT 4 cut(s) 57, 453, 522, 1114
TaqI TCGA 2 cut(s) 589, 707
TasI AATT 3 cut(s) 99, 910, 926
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 3 cut(s) 41, 334, 1049
Tru1I TTAA 7 cut(s) 135, 495, 675, 939, 1131, 1137, 1152
Tru9I TTAA 7 cut(s) 135, 495, 675, 939, 1131, 1137, 1152
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 811, 1040
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 6 cut(s) 101, 110, 684, 917, 1041, 1161
TspGWI ACGGA 3 cut(s) 1022, 1122, 1203
TspMI CCCGGG 1 cut(s) 823
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 549, 1057
XapI RAATTY 2 cut(s) 99, 926
XceI RCATGY 1 cut(s) 1199
XhoI CTCGAG 1 cut(s) 706
XmaI CCCGGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.