Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005949

Physical Location & Seq

Genomic Coordinates Forward (+)

27977381 .. 27981604

Gene Structure 4224 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1189400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGGCTTCAGTGGGTGTGGCGCCTACTTCAGGTAGGAGAGAACCCAGTGGTCATGCGGTTGGTGTGGATAAGTTGCCGGAGGAGATGAATGACATGAAAATTAGGGATGACAAAGAAATGGAATCCACAGTCGCAGTTGTTGACGGTAACGGAACAGAGACAGGCCATATAATCGTGACAACTATTGGTGGTAGAAATGGCCAGCCAAAACAGACAATTAGTTACATGGCTGAGCGTGTTGTCGGACACGGATCATTTGGAGTTGTGTTCCAAGCAAAGTGCTTAGAGACTGGTGAAACTGTGGCTATTAAGAAGGTTTTGCAAGATAAGAGGTACAAGAATCGTGAGCTACAGACCATGCGCCTTCTTGACCACCCAAATGTTGTCTCTTTGAAGCATTGCTTCTTTTCAACGACGGAAAAGGATGAACTCTATCTTAACTTGGTGCTTGAGTATGTTCCTGAAACGGTTCACCGTGTGATTAAACACTACAACAAGTTGAACCAACGGATGCCTCTTATATATGTTAAACTCTACACATACCAGATCTTTAGGGCGTTATCCTACATTCACCGCTGCATTGGAGTGTGTCATCGGGACATTAAGCCTCAAAATCTTCTGGTGAACCCACATACCCACCAAGTAAAGTTGTGCGACTTTGGAAGTGCCAAAGTTTTGGTAAAAGGAGAGCCAAATATATCTTATATCTGCTCTAGATATTATAGAGCACCGGAACTTATATTTGGAGCAACTGAGTATACTTCAGCTATTGATGTTTGGTCTGTTGGGTGTGTTCTTGCTGAGCTATTGCTCGGACAGCCTCTCTTTCCTGGTGAGAGTGGAGTTGACCAGCTTGTTGAGATTATCAAGGTTTTGGGCACTCCAACACGGGAGGAAATCAAATGCATGAACCCTAACTATACAGAGTTCAAATTTCCTCAAATCAAAGCTCACCCGTGGCACAAGATATTCCACAAGCGTATGCCTCCAGAAGCTGTTGATCTGGTTTCAAGACTGCTGCAATACTCTCCTAACCTGCGGTGCACAGCTCTGGATGCCTTGGTCCACTCATTCTTTGATGATCTTCGCGACCCAAACACCCGCCTGCCGAATGGACGCTTCCTTCCGCCTTTATTCAACTTTAAATCACATGAATTGAAGGGAGTGCCTGCGGAAACTTTGATGAAATTGGTTCCAGAACACGTGCGAAAGCAAGTCCCGTTCTTGGCATCCGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009933 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032502 GO:0036211 GO:0042538 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0047484 GO:0048507 GO:0048518 GO:0048532 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050896 GO:0065007 GO:0071704 GO:0080134 GO:0140096 GO:1901000 GO:1901002 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko01521 ko04012 ko04062 ko04110 ko04150 ko04151 ko04310 ko04340 ko04341 ko04360 ko04390 ko04510 ko04550 ko04657 ko04660 ko04662 ko04711 ko04722 ko04728 ko04910 ko04916 ko04917 ko04919 ko04931 ko04932 ko04934 ko05010 ko05160 ko05162 ko05164 ko05165 ko05166 ko05167 ko05169 ko05200 ko05210 ko05213 ko05215 ko05217 ko05224 ko05225 ko05226 map01521 map04012 map04062 map04110 map04150 map04151 map04310 map04340 map04341 map04360 map04390 map04510 map04550 map04657 map04660 map04662 map04711 map04722 map04728 map04910 map04916 map04917 map04919 map04931 map04932 map04934 map05010 map05160 map05162 map05164 map05165 map05166 map05167 map05169 map05200 map05210 map05213 map05215 map05217 map05224 map05225 map05226

Pfam Domains

Protein Families

Pkinase

Protein Analysis

413

Amino Acids

46.73

Weight (kDa)

8.7

Isoelectric Point (pI)

35.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	76 - 359	2e-61	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	78 - 277	1e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05840 AT3G05840 AT5G26751
fragaria_vesca	FvH4_6g08170 FvH4_6g08170 FvH4_6g08170 FvH4_6g08170 FvH4_6g08170
malus_domestica	MD04G1189400.v1.1 MD12G1202200.v1.1
prunus_persica	Prupe.6G307000_v2.0.a1 Prupe.6G307000_v2.0.a1 Prupe.6G307000_v2.0.a1 Prupe.6G307000_v2.0.a1 Prupe.6G307000_v2.0.a1 Prupe.6G307000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0458041
rosa_multiflora	Rmu_sc0000384.1_g000013
rosa_roxburghii	Rroxscaffold_6G00421330
rosa_rugosa	Rorug03G0024500 Rorug03G0024600
rosa_samantha	Rh3AG085000 Rh3BG087800 Rh3CG088300 Rh3DG088700
rosa_wichuraiana	Rw0G012420 Rw3G007240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1044
AccB1I	GGYRCC	1 cut(s)	19
AccI	GTMKAC	1 cut(s)	758
AccII	CGCG	1 cut(s)	1089
AciI	CCGC	6 cut(s)	56, 574, 1039, 1102, 1127, 1172
AclWI	GGATC	1 cut(s)	259
AcoI	YGGCCR	1 cut(s)	199
AcsI	RAATTY	1 cut(s)	932
AcuI	CTGAAG	2 cut(s)	12, 747
AcvI	CACGTG	1 cut(s)	1204
AcyI	GRCGYC	1 cut(s)	20
AdeI	CACNNNGTG	1 cut(s)	478
AfaI	GTAC	1 cut(s)	335
AfiI	CCNNNNNNNGG	2 cut(s)	29, 1225
AflIII	ACRYGT	1 cut(s)	1201
AgsI	TTSAA	7 cut(s)	394, 411, 502, 931, 1011, 1138, 1159
AjnI	CCWGG	1 cut(s)	829
AjuI	GAANNNNNNNTTGG	2 cut(s)	726, 758
AluBI	AGCT	7 cut(s)	349, 767, 805, 853, 950, 995, 1049
AluI	AGCT	7 cut(s)	349, 767, 805, 853, 950, 995, 1049
Alw21I	GWGCWC	2 cut(s)	730, 1046
Alw26I	GTCTC	3 cut(s)	152, 281, 391
Alw44I	GTGCAC	1 cut(s)	1042
AlwI	GGATC	1 cut(s)	259
AlwNI	CAGNNNCTG	1 cut(s)	995
AoxI	GGCC	2 cut(s)	163, 199
ApaLI	GTGCAC	1 cut(s)	1042
ApeKI	GCWGC	2 cut(s)	576, 1018
ApoI	RAATTY	1 cut(s)	932
Asp700I	GAANNNNTTC	1 cut(s)	468
AspLEI	GCGC	2 cut(s)	22, 363
AspS9I	GGNCC	1 cut(s)	1063
AsuHPI	GGTGA	6 cut(s)	305, 464, 563, 634, 845, 944
AvaII	GGWCC	1 cut(s)	1063
BaeGI	GKGCMC	2 cut(s)	881, 1046
BalI	TGGCCA	1 cut(s)	201
BanI	GGYRCC	1 cut(s)	19
BbrPI	CACGTG	1 cut(s)	1204
Bbv12I	GWGCWC	2 cut(s)	730, 1046
BbvI	GCAGC	2 cut(s)	563, 1005
BciT130I	CCWGG	1 cut(s)	831
BcoDI	GTCTC	3 cut(s)	152, 281, 391
BfaI	CTAG	1 cut(s)	714
BfmI	CTRYAG	1 cut(s)	350
BfoI	RGCGCY	1 cut(s)	23
BfuAI	ACCTGC	1 cut(s)	1044
BglII	AGATCT	1 cut(s)	546
BisI	GCNGC	2 cut(s)	577, 1019
BlpI	GCTNAGC	2 cut(s)	231, 801
BlsI	GCNGC	2 cut(s)	578, 1020
Bme1390I	CCNGG	1 cut(s)	831
Bme18I	GGWCC	1 cut(s)	1063
BmgT120I	GGNCC	1 cut(s)	1063
BmiI	GGNNCC	2 cut(s)	21, 1194
BmrFI	CCNGG	1 cut(s)	831
BmrI	ACTGGG	1 cut(s)	39
BmsI	GCATC	2 cut(s)	501, 1045
BmuI	ACTGGG	1 cut(s)	39
BpmI	CTGGAG	1 cut(s)	972
Bpu1102I	GCTNAGC	2 cut(s)	231, 801
BpuEI	CTTGAG	1 cut(s)	470
BsaAI	YACGTR	1 cut(s)	1204
BsaHI	GRCGYC	1 cut(s)	20
BsaJI	CCNNGG	2 cut(s)	956, 1059
BsaWI	WCCGGW	1 cut(s)	730
Bsc4I	CCNNNNNNNGG	2 cut(s)	29, 1225
Bse1I	ACTGG	2 cut(s)	45, 295
Bse3DI	GCAATG	1 cut(s)	397
BseBI	CCWGG	1 cut(s)	831
BseDI	CCNNGG	2 cut(s)	956, 1059
BseGI	GGATG	5 cut(s)	112, 430, 516, 1060, 1229
BseLI	CCNNNNNNNGG	2 cut(s)	29, 1225
BseMI	GCAATG	1 cut(s)	397
BseMII	CTCAG	3 cut(s)	222, 744, 792
BseNI	ACTGG	2 cut(s)	45, 295
BseRI	GAGGAG	1 cut(s)	95
BseSI	GKGCMC	2 cut(s)	881, 1046
BseXI	GCAGC	2 cut(s)	563, 1005
Bsh1236I	CGCG	1 cut(s)	1089
BshFI	GGCC	2 cut(s)	165, 201
BshNI	GGYRCC	1 cut(s)	19
BsiHKAI	GWGCWC	2 cut(s)	730, 1046
BsiSI	CCGG	2 cut(s)	77, 731
BslFI	GGGAC	2 cut(s)	611, 1202
BslI	CCNNNNNNNGG	2 cut(s)	29, 1225
BsmAI	GTCTC	3 cut(s)	152, 281, 391
BsmFI	GGGAC	2 cut(s)	611, 1202
BsnI	GGCC	2 cut(s)	165, 201
Bsp1286I	GDGCHC	3 cut(s)	730, 881, 1046
Bsp143I	GATC	4 cut(s)	251, 546, 1000, 1081
Bsp1720I	GCTNAGC	2 cut(s)	231, 801
Bsp68I	TCGCGA	1 cut(s)	1089
BspACI	CCGC	6 cut(s)	56, 574, 1039, 1102, 1127, 1172
BspANI	GGCC	2 cut(s)	165, 201
BspCNI	CTCAG	3 cut(s)	223, 745, 793
BspFNI	CGCG	1 cut(s)	1089
BspLI	GGNNCC	2 cut(s)	21, 1194
BspMI	ACCTGC	1 cut(s)	1044
BspPI	GGATC	1 cut(s)	259
BspT107I	GGYRCC	1 cut(s)	19
BsrDI	GCAATG	1 cut(s)	397
BsrI	ACTGG	2 cut(s)	45, 295
BssECI	CCNNGG	2 cut(s)	956, 1059
BssMI	GATC	4 cut(s)	251, 546, 1000, 1081
BssNAI	GTATAC	1 cut(s)	759
BssNI	GRCGYC	1 cut(s)	20
BssT1I	CCWWGG	1 cut(s)	1059
Bst1107I	GTATAC	1 cut(s)	759
Bst2UI	CCWGG	1 cut(s)	831
Bst4CI	ACNGT	5 cut(s)	130, 146, 301, 469, 476
BstACI	GRCGYC	1 cut(s)	20
BstBAI	YACGTR	1 cut(s)	1204
BstC8I	GCNNGC	3 cut(s)	203, 1106, 1170
BstDEI	CTNAG	4 cut(s)	231, 283, 753, 801
BstDSI	CCRYGG	1 cut(s)	956
BstENI	CCTNNNNNAGG	1 cut(s)	27
BstF5I	GGATG	5 cut(s)	112, 430, 516, 1060, 1229
BstFNI	CGCG	1 cut(s)	1089
BstH2I	RGCGCY	1 cut(s)	23
BstHHI	GCGC	2 cut(s)	22, 363
BstKTI	GATC	4 cut(s)	254, 549, 1003, 1084
BstMAI	GTCTC	3 cut(s)	152, 281, 391
BstMBI	GATC	4 cut(s)	251, 546, 1000, 1081
BstMWI	GCNNNNNNNGC	2 cut(s)	817, 1055
BstNI	CCWGG	1 cut(s)	831
BstSCI	CCNGG	1 cut(s)	829
BstSFI	CTRYAG	1 cut(s)	350
BstSLI	GKGCMC	2 cut(s)	881, 1046
BstUI	CGCG	1 cut(s)	1089
BstV1I	GCAGC	2 cut(s)	563, 1005
BstX2I	RGATCY	1 cut(s)	546
BstXI	CCANNNNNNTGG	1 cut(s)	676
BstYI	RGATCY	1 cut(s)	546
BstZ17I	GTATAC	1 cut(s)	759
BsuRI	GGCC	2 cut(s)	165, 201
BtgI	CCRYGG	1 cut(s)	956
BtsCI	GGATG	5 cut(s)	112, 430, 516, 1060, 1229
BtsIMutI	CAGTG	2 cut(s)	15, 52
BtuMI	TCGCGA	1 cut(s)	1089
BveI	ACCTGC	1 cut(s)	1044
Cac8I	GCNNGC	3 cut(s)	203, 1106, 1170
CaiI	CAGNNNCTG	1 cut(s)	995
CfoI	GCGC	2 cut(s)	22, 363
Cfr13I	GGNCC	1 cut(s)	1063
CseI	GACGC	1 cut(s)	1125
Csp6I	GTAC	1 cut(s)	334
CviAII	CATG	6 cut(s)	53, 94, 226, 358, 907, 1151
CviQI	GTAC	1 cut(s)	334
DdeI	CTNAG	4 cut(s)	231, 283, 753, 801
DinI	GGCGCC	1 cut(s)	21
DpnI	GATC	4 cut(s)	253, 548, 1002, 1083
DpnII	GATC	4 cut(s)	251, 546, 1000, 1081
DraI	TTTAAA	1 cut(s)	1144
DraIII	CACNNNGTG	1 cut(s)	478
EaeI	YGGCCR	1 cut(s)	199
EciI	GGCGGA	1 cut(s)	1116
Eco130I	CCWWGG	1 cut(s)	1059
Eco47I	GGWCC	1 cut(s)	1063
Eco57I	CTGAAG	2 cut(s)	12, 747
Eco72I	CACGTG	1 cut(s)	1204
EcoNI	CCTNNNNNAGG	1 cut(s)	27
EcoRII	CCWGG	1 cut(s)	829
EcoT14I	CCWWGG	1 cut(s)	1059
EcoT22I	ATGCAT	1 cut(s)	908
EgeI	GGCGCC	1 cut(s)	21
EheI	GGCGCC	1 cut(s)	21
ErhI	CCWWGG	1 cut(s)	1059
FaeI	CATG	6 cut(s)	56, 97, 229, 361, 910, 1154
FalI	AAGNNNNNCTT	2 cut(s)	386, 418
FaqI	GGGAC	2 cut(s)	611, 1202
FatI	CATG	6 cut(s)	52, 93, 225, 357, 906, 1150
FauI	CCCGC	1 cut(s)	1109
FblI	GTMKAC	1 cut(s)	758
Fnu4HI	GCNGC	2 cut(s)	577, 1019
FokI	GGATG	5 cut(s)	119, 437, 523, 1067, 1216
Fsp4HI	GCNGC	2 cut(s)	577, 1019
FspBI	CTAG	1 cut(s)	714
GlaI	GCGC	2 cut(s)	21, 362
GluI	GCNGC	2 cut(s)	577, 1019
GsuI	CTGGAG	1 cut(s)	972
HaeII	RGCGCY	1 cut(s)	23
HaeIII	GGCC	2 cut(s)	165, 201
HapII	CCGG	2 cut(s)	77, 731
HgaI	GACGC	1 cut(s)	1125
HhaI	GCGC	2 cut(s)	22, 363
Hin1I	GRCGYC	1 cut(s)	20
Hin1II	CATG	6 cut(s)	56, 97, 229, 361, 910, 1154
Hin6I	GCGC	2 cut(s)	20, 361
HinP1I	GCGC	2 cut(s)	20, 361
HincII	GTYRAC	2 cut(s)	142, 847
HindII	GTYRAC	2 cut(s)	142, 847
HinfI	GANTC	2 cut(s)	122, 340
HpaII	CCGG	2 cut(s)	77, 731
HphI	GGTGA	6 cut(s)	305, 464, 563, 634, 845, 944
Hpy166II	GTNNAC	7 cut(s)	142, 472, 625, 759, 847, 1044, 1066
Hpy188I	TCNGA	3 cut(s)	245, 815, 1234
Hpy8I	GTNNAC	7 cut(s)	142, 472, 625, 759, 847, 1044, 1066
Hpy99I	CGWCG	1 cut(s)	418
HpyAV	CCTTC	4 cut(s)	307, 374, 1133, 1153
HpyCH4III	ACNGT	5 cut(s)	130, 146, 301, 469, 476
HpyCH4IV	ACGT	1 cut(s)	1203
HpyCH4V	TGCA	5 cut(s)	322, 579, 906, 1021, 1044
HpyF10VI	GCNNNNNNNGC	2 cut(s)	817, 1055
HpyF3I	CTNAG	4 cut(s)	231, 283, 753, 801
HpySE526I	ACGT	1 cut(s)	1203
Hsp92I	GRCGYC	1 cut(s)	20
Hsp92II	CATG	6 cut(s)	56, 97, 229, 361, 910, 1154
HspAI	GCGC	2 cut(s)	20, 361
KasI	GGCGCC	1 cut(s)	19
Kzo9I	GATC	4 cut(s)	251, 546, 1000, 1081
LmnI	GCTCC	1 cut(s)	746
Lsp1109I	GCAGC	2 cut(s)	563, 1005
LweI	GCATC	2 cut(s)	501, 1045
MaeI	CTAG	1 cut(s)	714
MaeII	ACGT	1 cut(s)	1203
MaeIII	GTNAC	3 cut(s)	146, 175, 221
MalI	GATC	4 cut(s)	253, 548, 1002, 1083
MboI	GATC	4 cut(s)	251, 546, 1000, 1081
MboII	GAAGA	2 cut(s)	608, 1076
MflI	RGATCY	1 cut(s)	546
MhlI	GDGCHC	3 cut(s)	730, 881, 1046
MlsI	TGGCCA	1 cut(s)	201
MluCI	AATT	5 cut(s)	99, 216, 932, 1154, 1187
MluNI	TGGCCA	1 cut(s)	201
Mly113I	GGCGCC	1 cut(s)	20
MmeI	TCCRAC	2 cut(s)	223, 908
MnlI	CCTC	8 cut(s)	73, 324, 525, 618, 831, 886, 948, 996
Mox20I	TGGCCA	1 cut(s)	201
Mph1103I	ATGCAT	1 cut(s)	908
MroXI	GAANNNNTTC	1 cut(s)	468
MscI	TGGCCA	1 cut(s)	201
MseI	TTAA	6 cut(s)	309, 438, 483, 528, 603, 1143
MslI	CAYNNNNRTG	2 cut(s)	378, 584
Msp20I	TGGCCA	1 cut(s)	201
MspA1I	CMGCKG	1 cut(s)	576
MspI	CCGG	2 cut(s)	77, 731
MspR9I	CCNGG	1 cut(s)	831
MvaI	CCWGG	1 cut(s)	831
MvnI	CGCG	1 cut(s)	1089
MwoI	GCNNNNNNNGC	2 cut(s)	817, 1055
NarI	GGCGCC	1 cut(s)	20
NdeII	GATC	4 cut(s)	251, 546, 1000, 1081
NlaIII	CATG	6 cut(s)	56, 97, 229, 361, 910, 1154
NlaIV	GGNNCC	2 cut(s)	21, 1194
NmuCI	GTSAC	1 cut(s)	175
NruI	TCGCGA	1 cut(s)	1089
NsiI	ATGCAT	1 cut(s)	908
PdmI	GAANNNNTTC	1 cut(s)	468
PfeI	GAWTC	2 cut(s)	122, 340
PkrI	GCNGC	2 cut(s)	578, 1020
PluTI	GGCGCC	1 cut(s)	23
PmaCI	CACGTG	1 cut(s)	1204
PmlI	CACGTG	1 cut(s)	1204
Ppu21I	YACGTR	1 cut(s)	1204
Psp6I	CCWGG	1 cut(s)	829
PspCI	CACGTG	1 cut(s)	1204
PspGI	CCWGG	1 cut(s)	829
PspN4I	GGNNCC	2 cut(s)	21, 1194
PspPI	GGNCC	1 cut(s)	1063
PstNI	CAGNNNCTG	1 cut(s)	995
PsuI	RGATCY	1 cut(s)	546
RruI	TCGCGA	1 cut(s)	1089
RsaI	GTAC	1 cut(s)	335
RsaNI	GTAC	1 cut(s)	334
RseI	CAYNNNNRTG	2 cut(s)	378, 584
SaqAI	TTAA	6 cut(s)	309, 438, 483, 528, 603, 1143
SatI	GCNGC	2 cut(s)	577, 1019
Sau3AI	GATC	4 cut(s)	251, 546, 1000, 1081
Sau96I	GGNCC	1 cut(s)	1063
ScrFI	CCNGG	1 cut(s)	831
SduI	GDGCHC	3 cut(s)	730, 881, 1046
SfaNI	GCATC	2 cut(s)	501, 1045
SfcI	CTRYAG	1 cut(s)	350
SfoI	GGCGCC	1 cut(s)	21
SinI	GGWCC	1 cut(s)	1063
SmiMI	CAYNNNNRTG	2 cut(s)	378, 584
SmlI	CTYRAG	1 cut(s)	449
SmoI	CTYRAG	1 cut(s)	449
Sse9I	AATT	5 cut(s)	99, 216, 932, 1154, 1187
SsiI	CCGC	6 cut(s)	56, 574, 1039, 1102, 1127, 1172
SspDI	GGCGCC	1 cut(s)	19
SspMI	CTAG	1 cut(s)	714
StyD4I	CCNGG	1 cut(s)	829
StyI	CCWWGG	1 cut(s)	1059
TaaI	ACNGT	5 cut(s)	130, 146, 301, 469, 476
TaiI	ACGT	1 cut(s)	1206
TasI	AATT	5 cut(s)	99, 216, 932, 1154, 1187
TfiI	GAWTC	2 cut(s)	122, 340
Tru1I	TTAA	6 cut(s)	309, 438, 483, 528, 603, 1143
Tru9I	TTAA	6 cut(s)	309, 438, 483, 528, 603, 1143
TscAI	CASTG	2 cut(s)	15, 52
TseFI	GTSAC	1 cut(s)	175
TseI	GCWGC	2 cut(s)	576, 1018
Tsp45I	GTSAC	1 cut(s)	175
TspDTI	ATGAA	6 cut(s)	101, 110, 441, 923, 1167, 1199
TspGWI	ACGGA	4 cut(s)	165, 264, 431, 523
TspRI	CASTG	2 cut(s)	15, 52
VneI	GTGCAC	1 cut(s)	1042
VpaK11BI	GGWCC	1 cut(s)	1063
XagI	CCTNNNNNAGG	1 cut(s)	27
XapI	RAATTY	1 cut(s)	932
XbaI	TCTAGA	1 cut(s)	713
XmiI	GTMKAC	1 cut(s)	758
XmnI	GAANNNNTTC	1 cut(s)	468
XspI	CTAG	1 cut(s)	714
Zsp2I	ATGCAT	1 cut(s)	908

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.