Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008568

Physical Location & Seq

Genomic Coordinates Reverse (-)

2079033 .. 2083472

Gene Structure 4440 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G06650.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGGCTACGGGACAGCTATTTTCACGCAACACACAAGCGTTGTTCTACAATTATAAGCAGCTTCCCATTCAGCGTATGCTTGATTTTGATTTCCTCTGTGGGCGAGAAACTCCTTCTGTTGCTGGTATCATAAACCCAGGTTCCGAGGGATTCCAAAAGCTATTTTTTGGTCAGGAGGAGATCGCCATCCCTGTCCACGCAGCCATTGAGGCAGCATGCGCTGCGCATCCCACAGCAGATGTATTCATCAACTTCGCATCGTTTAGAAGTGCTGCTGCTTCATCCATGGCTGCTTTGAAGCAGCCAACTATTAAAGTAGTGGCCATTATAGCAGAAGGTGTTCCGGAGTCAGACACTAAGCAGTTGATTGCTTATGCTCGTGCTAATAATAAGGTTATTATTGGACCAGCTACTGTTGGAGGCGTTCAAGCTGGAGCCTTTAAAATTGGTGACACAGCTGGAACTATTGACAATATCATCCAATGCAAGTTGTACAGGCCTGGATCTGTTGGCTTTGTCTCAAAATCTGGTGGCATGTCTAATGAAATGTACAATACCATTGCCCGTGTGACAGATGGGATCTATGAAGGCATTGCTATTGGTGGGGATGTGTTCCCGGGCTCAACTTTATCTGACCATATCCTGCGGTTTAACAACATCCCACAGATTAAGATGGTGGTTGTACTTGGAGAGCTTGGAGGAAGAGATGAATATTCTCTAGTTGAAGCCATGAAACAGGGAAAAGTGACCAAACCAGTGGTTGCTTGGGTCAGCGGGACTTGTGCACGTCTATTCAAGTCCGAAGTACAATTTGGTCATGCAGGGGCCAAAAGTGGTGGAGAGATGGAGTCTGCACAAGCCAAGAATCAAGCGCTACAAGATGCTGGAGCTACTGTACCCACTTCATTCGAAGCCCTAGAAGTTGCAATCAAGGAAACTTTTGACAAATTGGTTGAAGAGGGAAAGGTCTCTCCTATTAAGGAAGTCACTCCTCCACAAATCCCTGAAGATCTCAGCTCTGCAATTAAGAGTGGGAAAGTCCGGGCTCCTACTCACATCATCTCCACCATTTCTGATGACAGAGGGGAGGAACCATGCTATGCTGGTGTGCCAATGTCATCCATCATTGAACAGGGTTATGGCGTGGGAGATGTCATTTCCCTCCTATGGTTCAAACGTAGTCTTCCTCGATATTGTACCAAATTCATCGAGATATGCATAATGTTGTGCGCTGACCACGGTCCATGCGTCTCTGGCGCTCACAACACCATCGTAACAGCAAGAGCAGGAAAAGACCTTGTCTCAAGTCTTGTCTCAGGTCTATTAACAATCGGTCCTAGATTTGGTGGTGCCATTGATGATGCAGCACGATACTTCAAGGACGCTTGTGACAGGAATCTCACACCTTATGAATTCGTGGAAGGCATGAAGAAGAAAGGAATCCGTGTCCCTGGCATTGGTCACAGGATAAAGAGCAGAGACAACAGAGACAAAAGAGTGGAGCTGCTTCAGAAATTTGCACGGTCTAACTTCCCTGCAGTGAAGTACATGGAATACGCAGTCCAAGTAGAGACATACACACTGTCTAAAGCCAACAACCTGGTACTCAACGTCGATGGAGCCATTGGATCTCTCTTCTTAGACCTTCTCGCTGGAAGTGGGATGTTCACAAAACAAGAGATAGACGAAATAGTTCAGATCGGTTATCTCAACGGCCTCTTCGTCCTCGCTCGATCCATCGGTTTAATCGGGCACACATTCGATCAGAAGAGACTGAAGCAGCCACTGTACCGACACCCGTGGGAAGATGTCTTGTACACCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016020 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

Citrate_synt CoA_binding Ligase_CoA

Protein Analysis

608

Amino Acids

65.81

Weight (kDa)

7.92

Isoelectric Point (pI)

36.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ligase_CoA	PF00549	173 - 297	1.5e-18	CoA-ligase
Citrate_synt	PF00285	400 - 597	1.1e-16	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008568)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06650 AT3G06650 AT5G49460 AT5G49460
fragaria_vesca	FvH4_4g19740 FvH4_4g19740 FvH4_4g19740 FvH4_4g19740
malus_domestica	MD13G1153500.v1.1 MD16G1154000.v1.1
prunus_persica	Prupe.1G117200_v2.0.a1 Prupe.1G117200_v2.0.a1
pyrus_communis	pycom13g13120 pycom16g13050
rosa_chinensis	RchiOBHm_Chr4g0425291
rosa_laevigata	RLG00000007382
rosa_multiflora	Rmu_sc0009125.1_g000003
rosa_roxburghii	Rroxscaffold_5G00367290
rosa_rugosa	Rorug04G0202100 Rorug04G0202200 Rorug04G0202300
rosa_samantha	Rh4CG276200
rosa_wichuraiana	Rw4G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	54
Acc16I	TGCGCA	1 cut(s)	225
AccB1I	GGYRCC	1 cut(s)	1349
AccIII	TCCGGA	1 cut(s)	343
AciI	CCGC	2 cut(s)	646, 774
AclWI	GGATC	4 cut(s)	511, 587, 1636, 1728
AcoI	YGGCCR	1 cut(s)	321
AcsI	RAATTY	3 cut(s)	1202, 1412, 1514
AcuI	CTGAAG	3 cut(s)	1026, 1493, 1796
AfeI	AGCGCT	1 cut(s)	873
AfiI	CCNNNNNNNGG	3 cut(s)	1167, 1343, 1457
AgsI	TTSAA	8 cut(s)	298, 428, 725, 796, 956, 1130, 1174, 1378
AjiI	CACGTC	1 cut(s)	788
AjnI	CCWGG	4 cut(s)	136, 499, 1450, 1599
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
Alw21I	GWGCWC	1 cut(s)	787
Alw26I	GTCTC	9 cut(s)	523, 973, 1255, 1306, 1318, 1473, 1482, 1565, 1765
Alw44I	GTGCAC	1 cut(s)	783
AlwI	GGATC	4 cut(s)	511, 587, 1636, 1728
AlwNI	CAGNNNCTG	2 cut(s)	413, 1786
Ama87I	CYCGRG	1 cut(s)	616
Aor13HI	TCCGGA	1 cut(s)	343
Aor51HI	AGCGCT	1 cut(s)	873
AoxI	GGCC	4 cut(s)	321, 497, 825, 1714
ApaLI	GTGCAC	1 cut(s)	783
ApoI	RAATTY	3 cut(s)	1202, 1412, 1514
Asp700I	GAANNNNTTC	2 cut(s)	339, 1692
AspLEI	GCGC	5 cut(s)	221, 226, 874, 1232, 1259
AspS9I	GGNCC	4 cut(s)	404, 825, 1241, 1334
AsuC2I	CCSGG	3 cut(s)	617, 618, 1043
AsuHPI	GGTGA	1 cut(s)	461
AsuII	TTCGAA	1 cut(s)	909
AvaI	CYCGRG	1 cut(s)	616
AvaII	GGWCC	3 cut(s)	404, 1241, 1334
BaeGI	GKGCMC	2 cut(s)	787, 1755
BalI	TGGCCA	1 cut(s)	323
BanI	GGYRCC	1 cut(s)	1349
BanII	GRGCYC	2 cut(s)	623, 1048
BauI	CACGAG	1 cut(s)	378
BbsI	GAAGAC	1 cut(s)	1175
Bbv12I	GWGCWC	1 cut(s)	787
BccI	CCATC	8 cut(s)	194, 569, 667, 838, 1130, 1277, 1610, 1745
BceAI	ACGGC	1 cut(s)	1729
BciT130I	CCWGG	4 cut(s)	138, 501, 1452, 1601
BcnI	CCSGG	3 cut(s)	617, 618, 1043
BcoDI	GTCTC	9 cut(s)	523, 973, 1255, 1306, 1318, 1473, 1482, 1565, 1765
BfaI	CTAG	3 cut(s)	719, 917, 1338
BfmI	CTRYAG	1 cut(s)	1536
BfoI	RGCGCY	2 cut(s)	875, 1260
BglI	GCCNNNNNGGC	1 cut(s)	209
BglII	AGATCT	1 cut(s)	1009
Bme1390I	CCNGG	7 cut(s)	138, 501, 617, 618, 1043, 1452, 1601
Bme18I	GGWCC	3 cut(s)	404, 1241, 1334
BmeT110I	CYCGRG	1 cut(s)	616
BmgBI	CACGTC	1 cut(s)	788
BmgT120I	GGNCC	4 cut(s)	404, 825, 1241, 1334
BmiI	GGNNCC	7 cut(s)	142, 436, 826, 1047, 1092, 1351, 1621
BmrFI	CCNGG	7 cut(s)	138, 501, 617, 618, 1043, 1452, 1601
BmsI	GCATC	4 cut(s)	235, 266, 871, 1351
BoxI	GACNNNNGTC	1 cut(s)	1239
BpiI	GAAGAC	1 cut(s)	1175
BpmI	CTGGAG	2 cut(s)	453, 906
Bpu14I	TTCGAA	1 cut(s)	909
BpuEI	CTTGAG	1 cut(s)	1288
BpuMI	CCSGG	3 cut(s)	617, 618, 1043
BsaBI	GATNNNNATC	1 cut(s)	185
BsaI	GGTCTC	1 cut(s)	973
BsaJI	CCNNGG	7 cut(s)	136, 144, 285, 616, 1237, 1450, 1799
BsaWI	WCCGGW	1 cut(s)	343
BsaXI	ACNNNNNCTCC	4 cut(s)	879, 909, 1046, 1076
Bsc4I	CCNNNNNNNGG	3 cut(s)	1167, 1343, 1457
Bse1I	ACTGG	1 cut(s)	755
Bse3DI	GCAATG	2 cut(s)	558, 591
Bse8I	GATNNNNATC	1 cut(s)	185
BseAI	TCCGGA	1 cut(s)	343
BseBI	CCWGG	4 cut(s)	138, 501, 1452, 1601
BseDI	CCNNGG	7 cut(s)	136, 144, 285, 616, 1237, 1450, 1799
BseGI	GGATG	8 cut(s)	186, 226, 281, 477, 613, 657, 1118, 1668
BseJI	GATNNNNATC	1 cut(s)	185
BseLI	CCNNNNNNNGG	3 cut(s)	1167, 1343, 1457
BseMI	GCAATG	2 cut(s)	558, 591
BseMII	CTCAG	2 cut(s)	1027, 1329
BseNI	ACTGG	1 cut(s)	755
BseRI	GAGGAG	2 cut(s)	191, 981
BseSI	GKGCMC	2 cut(s)	787, 1755
BsgI	GTGCAG	1 cut(s)	837
BshFI	GGCC	4 cut(s)	323, 499, 827, 1716
BshNI	GGYRCC	1 cut(s)	1349
BsiHKAI	GWGCWC	1 cut(s)	787
BsiHKCI	CYCGRG	1 cut(s)	616
BsiSI	CCGG	3 cut(s)	344, 617, 1042
BslFI	GGGAC	3 cut(s)	24, 790, 1433
BslI	CCNNNNNNNGG	3 cut(s)	1167, 1343, 1457
BsmAI	GTCTC	9 cut(s)	523, 973, 1255, 1306, 1318, 1473, 1482, 1565, 1765
BsmBI	CGTCTC	1 cut(s)	1255
BsmFI	GGGAC	3 cut(s)	24, 790, 1433
BsnI	GGCC	4 cut(s)	323, 499, 827, 1716
Bso31I	GGTCTC	1 cut(s)	973
BsoBI	CYCGRG	1 cut(s)	616
Bsp119I	TTCGAA	1 cut(s)	909
Bsp1286I	GDGCHC	4 cut(s)	623, 787, 1048, 1755
Bsp13I	TCCGGA	1 cut(s)	343
Bsp1407I	TGTACA	3 cut(s)	492, 549, 1815
Bsp143I	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
Bsp19I	CCATGG	1 cut(s)	285
BspACI	CCGC	2 cut(s)	646, 774
BspANI	GGCC	4 cut(s)	323, 499, 827, 1716
BspCNI	CTCAG	2 cut(s)	1026, 1328
BspEI	TCCGGA	1 cut(s)	343
BspLI	GGNNCC	7 cut(s)	142, 436, 826, 1047, 1092, 1351, 1621
BspMAI	CTGCAG	1 cut(s)	1540
BspPI	GGATC	4 cut(s)	511, 587, 1636, 1728
BspT104I	TTCGAA	1 cut(s)	909
BspT107I	GGYRCC	1 cut(s)	1349
BspTNI	GGTCTC	1 cut(s)	973
BsrDI	GCAATG	2 cut(s)	558, 591
BsrGI	TGTACA	3 cut(s)	492, 549, 1815
BsrI	ACTGG	1 cut(s)	755
BssECI	CCNNGG	7 cut(s)	136, 144, 285, 616, 1237, 1450, 1799
BssMI	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
BssSI	CACGAG	1 cut(s)	378
BssT1I	CCWWGG	1 cut(s)	285
Bst2BI	CACGAG	1 cut(s)	378
Bst2UI	CCWGG	4 cut(s)	138, 501, 1452, 1601
Bst4CI	ACNGT	6 cut(s)	415, 895, 1241, 1524, 1584, 1788
Bst6I	CTCTTC	5 cut(s)	697, 951, 1640, 1724, 1763
BstAPI	GCANNNNNTGC	1 cut(s)	221
BstAUI	TGTACA	3 cut(s)	492, 549, 1815
BstBI	TTCGAA	1 cut(s)	909
BstC8I	GCNNGC	1 cut(s)	217
BstDEI	CTNAG	4 cut(s)	357, 1013, 1315, 1639
BstDSI	CCRYGG	3 cut(s)	285, 1237, 1799
BstF5I	GGATG	8 cut(s)	186, 226, 281, 477, 613, 657, 1118, 1668
BstH2I	RGCGCY	2 cut(s)	875, 1260
BstHHI	GCGC	5 cut(s)	221, 226, 874, 1232, 1259
BstKTI	GATC	8 cut(s)	183, 506, 582, 1012, 1631, 1701, 1736, 1765
BstMAI	GTCTC	9 cut(s)	523, 973, 1255, 1306, 1318, 1473, 1482, 1565, 1765
BstMBI	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
BstMWI	GCNNNNNNNGC	5 cut(s)	209, 218, 221, 329, 1254
BstNI	CCWGG	4 cut(s)	138, 501, 1452, 1601
BstNSI	RCATGY	2 cut(s)	219, 538
BstPAI	GACNNNNGTC	1 cut(s)	1239
BstSCI	CCNGG	7 cut(s)	136, 499, 615, 616, 1041, 1450, 1599
BstSFI	CTRYAG	1 cut(s)	1536
BstSLI	GKGCMC	2 cut(s)	787, 1755
BstV2I	GAAGAC	1 cut(s)	1175
BstX2I	RGATCY	4 cut(s)	503, 579, 1009, 1628
BstXI	CCANNNNNNTGG	2 cut(s)	757, 1600
BstYI	RGATCY	4 cut(s)	503, 579, 1009, 1628
BsuRI	GGCC	4 cut(s)	323, 499, 827, 1716
BtgI	CCRYGG	3 cut(s)	285, 1237, 1799
BtrI	CACGTC	1 cut(s)	788
BtsCI	GGATG	8 cut(s)	186, 226, 281, 477, 613, 657, 1118, 1668
BtsI	GCAGTG	1 cut(s)	1545
BtsIMutI	CAGTG	4 cut(s)	762, 1545, 1580, 1784
Cac8I	GCNNGC	1 cut(s)	217
CaiI	CAGNNNCTG	2 cut(s)	413, 1786
CfoI	GCGC	5 cut(s)	221, 226, 874, 1232, 1259
Cfr13I	GGNCC	4 cut(s)	404, 825, 1241, 1334
Cfr9I	CCCGGG	1 cut(s)	616
CseI	GACGC	2 cut(s)	1237, 1391
CsiI	ACCWGGT	1 cut(s)	1599
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviAII	CATG	9 cut(s)	216, 286, 535, 730, 818, 1095, 1245, 1426, 1549
DdeI	CTNAG	4 cut(s)	357, 1013, 1315, 1639
DpnI	GATC	8 cut(s)	182, 505, 581, 1011, 1630, 1700, 1735, 1764
DpnII	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
DraI	TTTAAA	1 cut(s)	442
EaeI	YGGCCR	1 cut(s)	321
Eam1104I	CTCTTC	5 cut(s)	697, 951, 1640, 1724, 1763
EarI	CTCTTC	5 cut(s)	697, 951, 1640, 1724, 1763
Eco130I	CCWWGG	1 cut(s)	285
Eco147I	AGGCCT	1 cut(s)	499
Eco24I	GRGCYC	2 cut(s)	623, 1048
Eco31I	GGTCTC	1 cut(s)	973
Eco47I	GGWCC	3 cut(s)	404, 1241, 1334
Eco47III	AGCGCT	1 cut(s)	873
Eco57I	CTGAAG	3 cut(s)	1026, 1493, 1796
Eco88I	CYCGRG	1 cut(s)	616
EcoRI	GAATTC	1 cut(s)	1412
EcoRII	CCWGG	4 cut(s)	136, 499, 1450, 1599
EcoT14I	CCWWGG	1 cut(s)	285
EcoT22I	ATGCAT	1 cut(s)	1220
EcoT38I	GRGCYC	2 cut(s)	623, 1048
ErhI	CCWWGG	1 cut(s)	285
Esp3I	CGTCTC	1 cut(s)	1255
FaeI	CATG	9 cut(s)	219, 289, 538, 733, 821, 1098, 1248, 1429, 1552
FaqI	GGGAC	3 cut(s)	24, 790, 1433
FatI	CATG	9 cut(s)	215, 285, 534, 729, 817, 1094, 1244, 1425, 1548
FauI	CCCGC	1 cut(s)	767
FokI	GGATG	8 cut(s)	173, 213, 268, 464, 620, 644, 1105, 1675
FriOI	GRGCYC	2 cut(s)	623, 1048
FspBI	CTAG	3 cut(s)	719, 917, 1338
FspI	TGCGCA	1 cut(s)	225
GlaI	GCGC	5 cut(s)	220, 225, 873, 1231, 1258
GsuI	CTGGAG	2 cut(s)	453, 906
HaeII	RGCGCY	2 cut(s)	875, 1260
HaeIII	GGCC	4 cut(s)	323, 499, 827, 1716
HapII	CCGG	3 cut(s)	344, 617, 1042
HgaI	GACGC	2 cut(s)	1237, 1391
HhaI	GCGC	5 cut(s)	221, 226, 874, 1232, 1259
Hin1II	CATG	9 cut(s)	219, 289, 538, 733, 821, 1098, 1248, 1429, 1552
Hin6I	GCGC	5 cut(s)	219, 224, 872, 1230, 1257
HinP1I	GCGC	5 cut(s)	219, 224, 872, 1230, 1257
HinfI	GANTC	6 cut(s)	150, 347, 848, 865, 1396, 1440
HpaII	CCGG	3 cut(s)	344, 617, 1042
HphI	GGTGA	1 cut(s)	461
Hpy166II	GTNNAC	4 cut(s)	196, 785, 1668, 1818
Hpy188I	TCNGA	8 cut(s)	145, 352, 634, 802, 1075, 1512, 1698, 1767
Hpy188III	TCNNGA	3 cut(s)	173, 344, 1210
Hpy8I	GTNNAC	4 cut(s)	196, 785, 1668, 1818
Hpy99I	CGWCG	1 cut(s)	1616
HpyAV	CCTTC	5 cut(s)	123, 329, 581, 1415, 1655
HpyCH4III	ACNGT	6 cut(s)	415, 895, 1241, 1524, 1584, 1788
HpyCH4IV	ACGT	3 cut(s)	787, 1177, 1611
HpyF10VI	GCNNNNNNNGC	5 cut(s)	209, 218, 221, 329, 1254
HpyF3I	CTNAG	4 cut(s)	357, 1013, 1315, 1639
HpySE526I	ACGT	3 cut(s)	787, 1177, 1611
Hsp92II	CATG	9 cut(s)	219, 289, 538, 733, 821, 1098, 1248, 1429, 1552
HspAI	GCGC	5 cut(s)	219, 224, 872, 1230, 1257
Kpn2I	TCCGGA	1 cut(s)	343
Kzo9I	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
LmnI	GCTCC	5 cut(s)	434, 887, 1051, 1501, 1619
LweI	GCATC	4 cut(s)	235, 266, 871, 1351
MabI	ACCWGGT	1 cut(s)	1599
MaeI	CTAG	3 cut(s)	719, 917, 1338
MaeII	ACGT	3 cut(s)	787, 1177, 1611
MaeIII	GTNAC	7 cut(s)	449, 568, 745, 985, 1273, 1388, 1460
MalI	GATC	8 cut(s)	182, 505, 581, 1011, 1630, 1700, 1735, 1764
MboI	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
MflI	RGATCY	4 cut(s)	503, 579, 1009, 1628
MhlI	GDGCHC	4 cut(s)	623, 787, 1048, 1755
MlsI	TGGCCA	1 cut(s)	323
MluCI	AATT	8 cut(s)	49, 444, 809, 947, 1023, 1202, 1412, 1514
MluNI	TGGCCA	1 cut(s)	323
MlyI	GAGTC	2 cut(s)	356, 857
MmeI	TCCRAC	1 cut(s)	397
Mox20I	TGGCCA	1 cut(s)	323
Mph1103I	ATGCAT	1 cut(s)	1220
MroI	TCCGGA	1 cut(s)	343
MroXI	GAANNNNTTC	2 cut(s)	339, 1692
MscI	TGGCCA	1 cut(s)	323
MseI	TTAA	8 cut(s)	312, 441, 651, 669, 978, 1026, 1325, 1745
Msp20I	TGGCCA	1 cut(s)	323
MspA1I	CMGCKG	2 cut(s)	458, 774
MspI	CCGG	3 cut(s)	344, 617, 1042
MspR9I	CCNGG	7 cut(s)	138, 501, 617, 618, 1043, 1452, 1601
MteI	GCGCNGCGC	1 cut(s)	222
MvaI	CCWGG	4 cut(s)	138, 501, 1452, 1601
MwoI	GCNNNNNNNGC	5 cut(s)	209, 218, 221, 329, 1254
NciI	CCSGG	3 cut(s)	617, 618, 1043
NcoI	CCATGG	1 cut(s)	285
NdeII	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
NlaIII	CATG	9 cut(s)	219, 289, 538, 733, 821, 1098, 1248, 1429, 1552
NlaIV	GGNNCC	7 cut(s)	142, 436, 826, 1047, 1092, 1351, 1621
NmuCI	GTSAC	6 cut(s)	449, 568, 745, 985, 1388, 1460
NsbI	TGCGCA	1 cut(s)	225
NsiI	ATGCAT	1 cut(s)	1220
NspI	RCATGY	2 cut(s)	219, 538
NspV	TTCGAA	1 cut(s)	909
PaeI	GCATGC	1 cut(s)	219
PceI	AGGCCT	1 cut(s)	499
PcsI	WCGNNNNNNNCGW	2 cut(s)	1245, 1719
PdmI	GAANNNNTTC	2 cut(s)	339, 1692
PfeI	GAWTC	4 cut(s)	150, 865, 1396, 1440
PflFI	GACNNNGTC	1 cut(s)	1298
PleI	GAGTC	2 cut(s)	355, 856
PpsI	GAGTC	2 cut(s)	355, 856
PshAI	GACNNNNGTC	1 cut(s)	1239
PsiI	TTATAA	1 cut(s)	54
Psp6I	CCWGG	4 cut(s)	136, 499, 1450, 1599
PspGI	CCWGG	4 cut(s)	136, 499, 1450, 1599
PspN4I	GGNNCC	7 cut(s)	142, 436, 826, 1047, 1092, 1351, 1621
PspPI	GGNCC	4 cut(s)	404, 825, 1241, 1334
PstI	CTGCAG	1 cut(s)	1540
PstNI	CAGNNNCTG	2 cut(s)	413, 1786
PsuI	RGATCY	4 cut(s)	503, 579, 1009, 1628
PsyI	GACNNNGTC	1 cut(s)	1298
PvuII	CAGCTG	1 cut(s)	458
SaqAI	TTAA	8 cut(s)	312, 441, 651, 669, 978, 1026, 1325, 1745
Sau3AI	GATC	8 cut(s)	180, 503, 579, 1009, 1628, 1698, 1733, 1762
Sau96I	GGNCC	4 cut(s)	404, 825, 1241, 1334
SchI	GAGTC	2 cut(s)	356, 857
ScrFI	CCNGG	7 cut(s)	138, 501, 617, 618, 1043, 1452, 1601
SduI	GDGCHC	4 cut(s)	623, 787, 1048, 1755
SexAI	ACCWGGT	1 cut(s)	1599
SfaNI	GCATC	4 cut(s)	235, 266, 871, 1351
SfcI	CTRYAG	1 cut(s)	1536
SfuI	TTCGAA	1 cut(s)	909
SinI	GGWCC	3 cut(s)	404, 1241, 1334
SmaI	CCCGGG	1 cut(s)	618
SmlI	CTYRAG	1 cut(s)	1303
SmoI	CTYRAG	1 cut(s)	1303
SphI	GCATGC	1 cut(s)	219
Sse9I	AATT	8 cut(s)	49, 444, 809, 947, 1023, 1202, 1412, 1514
SseBI	AGGCCT	1 cut(s)	499
SsiI	CCGC	2 cut(s)	646, 774
SspI	AATATT	1 cut(s)	713
SspMI	CTAG	3 cut(s)	719, 917, 1338
StuI	AGGCCT	1 cut(s)	499
StyD4I	CCNGG	7 cut(s)	136, 499, 615, 616, 1041, 1450, 1599
StyI	CCWWGG	1 cut(s)	285
TaaI	ACNGT	6 cut(s)	415, 895, 1241, 1524, 1584, 1788
TaiI	ACGT	3 cut(s)	790, 1180, 1614
TaqI	TCGA	6 cut(s)	909, 1189, 1209, 1614, 1732, 1761
TaqII	GACCGA	1 cut(s)	1322
TasI	AATT	8 cut(s)	49, 444, 809, 947, 1023, 1202, 1412, 1514
TatI	WGTACW	6 cut(s)	492, 549, 682, 805, 1545, 1815
TfiI	GAWTC	4 cut(s)	150, 865, 1396, 1440
Tru1I	TTAA	8 cut(s)	312, 441, 651, 669, 978, 1026, 1325, 1745
Tru9I	TTAA	8 cut(s)	312, 441, 651, 669, 978, 1026, 1325, 1745
TscAI	CASTG	4 cut(s)	762, 1545, 1587, 1791
TseFI	GTSAC	6 cut(s)	449, 568, 745, 985, 1388, 1460
Tsp45I	GTSAC	6 cut(s)	449, 568, 745, 985, 1388, 1460
TspGWI	ACGGA	1 cut(s)	1433
TspMI	CCCGGG	1 cut(s)	616
TspRI	CASTG	4 cut(s)	762, 1545, 1587, 1791
Tth111I	GACNNNGTC	1 cut(s)	1298
VneI	GTGCAC	1 cut(s)	783
VpaK11BI	GGWCC	3 cut(s)	404, 1241, 1334
XapI	RAATTY	3 cut(s)	1202, 1412, 1514
XceI	RCATGY	2 cut(s)	219, 538
XcmI	CCANNNNNNNNNTGG	1 cut(s)	762
XmaI	CCCGGG	1 cut(s)	616
XmnI	GAANNNNTTC	2 cut(s)	339, 1692
XspI	CTAG	3 cut(s)	719, 917, 1338
Zsp2I	ATGCAT	1 cut(s)	1220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.