Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0008568

Physical Location & Seq

Genomic Coordinates Forward (+)

23286810 .. 23292418

Gene Structure 5609 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g19740.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGGCCACCGGTCAATTGTTTTCCCGGACTACTCAAGCTTTGTTCTACAACTACAAGCAGCTCCCTATCCAGCGCATGCTTGATTTTGACTTCCTTTGCGGGAGGGAAACACCGTCTGTTGCCGGAATCATTAATCCCGGTGCTGAGGGATTTCAGAAGCTCTTTTTCGGCCAGGAGGAAATCGCCATACCAGTGCACTCAAGCATTGAGGCATCATGTGCTGCACATCCCACTGCCGATGTCTTTATCAACTTTGCTTCCTACAGAAGTGCTGCTGCTTCTTCCTTGACTGCTCTCAAGCAGCCAACCATCAGAGTCGTGGCCATTATTGCTGAGGGTGTGCCTGAGTCGGACGCTAAGCAGTTGATCGCATATGCACGAGCAAACAACAAGGTTGTCATTGGCCCAGCTACTGTTGGAGGAATCCAAGCTGGAGCTTTTAAGATTGGTGACACTGCCGGAACAATTGATAACATTATTCATTGCAAGCTATACAGGCCTGGATCTGTTGGTTTTGTCTCCAAATCCGGTGGGATGTCCAATGAAATGTACAATACTGTAGCTCGTGTAACAGACGGACTTTATGAAGGTATTGCTATTGGAGGTGATGTGTTCCCAGGTTCTACACTCTCAGACCATGTTCTGCGGTTTAACAATATCCCTCAGGTTAAAATTATTGTTGTACTTGGGGAACTTGGCGGGAGAGATGAGTATTCTCTAGTTGAAGCTTTAAAGCAGGGAAAAGTTAACAAACCAGTGGTTGCTTGGGTTAGTGGAACTTGTGCAACACTTTTCAAATCCGAAGTACAATTTGGACATGCTGGCGCTAAAAGTGGTGGTGAGATGGAGTCTGCACAAGCAAAGAACCAAGCCCTGAGGGATGCTGGAGCTGTTGTTCCCACTTCATATGAAGCTTTGGAAATTGCCATCAAGGAAACGTTTGAGAAATTGGTTGAAGAGGGGAAAATTGCACCAGTGAAGGAATTTAAGCCTCCACAGATACCTGAAGATCTTAACACCGCAATTAAGAGTGGAAAAGTTAGGGCTCCAACTCATATTATATCCACAATCTCTGATGATAGAGGTGAGGAACCATGCTATGCTGGTGTGCCTATGTCTTCCATTGTTGAGCAGGGTTATGGTGTGGGTGATGTTATCTCTCTTTTGTGGTTCAAACGCAGCCTTCCCAGTTACTGTACCAAGTTTATTGAGATATGTGTCATGTTGTGTGCTGACCATGGTCCTTGCGTCTCTGGTGCTCACAACACAATAGTAACCGCTAGGGCTGGCAAGGACCTAGTTTCCAGCCTTGTCTCAGGTTTGCTCACAATTGGTCCACGATTTGGTGGTGCTGTTGATGATGCTGCTCGATACTTCAAGGATGCATATGACAAGAATCTTACACCCTATGAGTTTGTCGAAAGCATGAAAAAGAGGGGAATCCGTGTGCCAGGGATTGGTCACAGGATCAAGAGAGGGGACAACAGAGATAAAAGAGTAGAGCTCCTACAACTGTTTGCACGCACACATTTCCCTTCTGTCAAGTACATGGAATACGCCGTAGAAGTTGAGACCTACACGCTCTCAAAGGCAAACAATCTGGTCCTGAATGTGGATGGTGCTATTGGAACCCTGTTCTTGGATCTCCTTGCTGGCAGTGGAATGTTCAACAAGCAAGAGATTGATGAGATTGTTGAGATTGGCTATCTGAATGGGCTATTTGTGCTGGCACGTTCCATTGGTTTGATTGGGCACACATTTGACCAGAAGAGGTTGAAGCAGCCCCTATACCGCCACCCATGGGAGGATGTTCTTTACACCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016020 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

Citrate_synt CoA_binding Ligase_CoA

Protein Analysis

609

Amino Acids

65.95

Weight (kDa)

6.87

Isoelectric Point (pI)

32.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Succ_CoA_lig	PF13607	167 - 306	5.3e-06	Succinyl-CoA ligase like flavodoxin domain
Ligase_CoA	PF00549	173 - 298	2e-19	CoA-ligase
Citrate_synt	PF00285	400 - 597	1.9e-16	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008568)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06650 AT3G06650 AT5G49460 AT5G49460
fragaria_vesca	FvH4_4g19740 FvH4_4g19740 FvH4_4g19740 FvH4_4g19740
malus_domestica	MD13G1153500.v1.1 MD16G1154000.v1.1
prunus_persica	Prupe.1G117200_v2.0.a1 Prupe.1G117200_v2.0.a1
pyrus_communis	pycom13g13120 pycom16g13050
rosa_chinensis	RchiOBHm_Chr4g0425291
rosa_laevigata	RLG00000007382
rosa_multiflora	Rmu_sc0009125.1_g000003
rosa_roxburghii	Rroxscaffold_5G00367290
rosa_rugosa	Rorug04G0202100 Rorug04G0202200 Rorug04G0202300
rosa_samantha	Rh4CG276200
rosa_wichuraiana	Rw4G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1343, 1457
AciI	CCGC	6 cut(s)	99, 646, 699, 1020, 1278, 1792
AclI	AACGTT	1 cut(s)	938
AclWI	GGATC	3 cut(s)	511, 1475, 1650
AcoI	YGGCCR	3 cut(s)	3, 169, 321
AcsI	RAATTY	1 cut(s)	983
AcuI	CTGAAG	1 cut(s)	1026
AdeI	CACNNNGTG	1 cut(s)	1824
AfaI	GTAC	5 cut(s)	551, 684, 807, 1198, 1547
AfiI	CCNNNNNNNGG	6 cut(s)	1343, 1457, 1612, 1639, 1801, 1804
AgeI	ACCGGT	1 cut(s)	8
AgsI	TTSAA	7 cut(s)	725, 796, 956, 1174, 1378, 1669, 1777
AjnI	CCWGG	4 cut(s)	171, 499, 616, 1450
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AloI	GAACNNNNNNTCC	2 cut(s)	879, 911
Alw21I	GWGCWC	3 cut(s)	198, 1261, 1506
Alw26I	GTCTC	4 cut(s)	523, 1255, 1318, 1565
Alw44I	GTGCAC	1 cut(s)	194
AlwI	GGATC	3 cut(s)	511, 1475, 1650
AlwNI	CAGNNNCTG	2 cut(s)	413, 1194
AoxI	GGCC	5 cut(s)	3, 169, 321, 403, 497
ApaLI	GTGCAC	1 cut(s)	194
ApeKI	GCWGC	8 cut(s)	58, 221, 272, 275, 301, 1179, 1364, 1780
ApoI	RAATTY	1 cut(s)	983
AseI	ATTAAT	1 cut(s)	132
AsiGI	ACCGGT	1 cut(s)	8
AspLEI	GCGC	2 cut(s)	75, 827
AspS9I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
AsuC2I	CCSGG	2 cut(s)	25, 138
AsuHPI	GGTGA	5 cut(s)	461, 617, 851, 1097, 1160
AvaII	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
AxyI	CCTNAGG	2 cut(s)	663, 875
BaeGI	GKGCMC	2 cut(s)	198, 1755
BalI	TGGCCA	2 cut(s)	5, 323
BanII	GRGCYC	2 cut(s)	1048, 1506
BauI	CACGAG	2 cut(s)	378, 564
BbsI	GAAGAC	1 cut(s)	1110
Bbv12I	GWGCWC	3 cut(s)	198, 1261, 1506
BbvCI	CCTCAGC	2 cut(s)	144, 333
BbvI	GCAGC	8 cut(s)	70, 208, 259, 262, 313, 1191, 1351, 1792
BccI	CCATC	4 cut(s)	317, 838, 935, 1610
BceAI	ACGGC	1 cut(s)	1544
BciT130I	CCWGG	4 cut(s)	173, 501, 618, 1452
BcnI	CCSGG	2 cut(s)	25, 138
BcoDI	GTCTC	4 cut(s)	523, 1255, 1318, 1565
BfaI	CTAG	3 cut(s)	719, 1281, 1298
BfmI	CTRYAG	2 cut(s)	262, 558
BfoI	RGCGCY	1 cut(s)	828
BglII	AGATCT	1 cut(s)	1009
BisI	GCNGC	8 cut(s)	59, 222, 273, 276, 302, 1180, 1365, 1781
BlpI	GCTNAGC	1 cut(s)	357
BlsI	GCNGC	8 cut(s)	60, 223, 274, 277, 303, 1181, 1366, 1782
Bme1390I	CCNGG	6 cut(s)	25, 138, 173, 501, 618, 1452
Bme18I	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
BmgT120I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
BmiI	GGNNCC	3 cut(s)	1047, 1092, 1630
BmrFI	CCNGG	6 cut(s)	25, 138, 173, 501, 618, 1452
BmrI	ACTGGG	1 cut(s)	1182
BmsI	GCATC	4 cut(s)	221, 871, 1351, 1372
BmuI	ACTGGG	1 cut(s)	1182
BoxI	GACNNNNGTC	1 cut(s)	1239
BpiI	GAAGAC	1 cut(s)	1110
BplI	GAGNNNNNCTC	2 cut(s)	1488, 1520
BpmI	CTGGAG	2 cut(s)	453, 906
Bpu10I	CCTNAGC	2 cut(s)	144, 333
Bpu1102I	GCTNAGC	1 cut(s)	357
BpuEI	CTTGAG	3 cut(s)	18, 184, 281
BpuMI	CCSGG	2 cut(s)	25, 138
BsaI	GGTCTC	1 cut(s)	1565
BsaJI	CCNNGG	4 cut(s)	616, 1237, 1451, 1799
BsaWI	WCCGGW	2 cut(s)	8, 527
BsaXI	ACNNNNNCTCC	4 cut(s)	594, 624, 879, 909
Bsc4I	CCNNNNNNNGG	6 cut(s)	1343, 1457, 1612, 1639, 1801, 1804
Bse118I	RCCGGY	1 cut(s)	8
Bse1I	ACTGG	4 cut(s)	191, 755, 974, 1188
Bse21I	CCTNAGG	2 cut(s)	663, 875
Bse3DI	GCAATG	1 cut(s)	481
BseBI	CCWGG	4 cut(s)	173, 501, 618, 1452
BseDI	CCNNGG	4 cut(s)	616, 1237, 1451, 1799
BseGI	GGATG	6 cut(s)	226, 540, 886, 1387, 1621, 1813
BseLI	CCNNNNNNNGG	6 cut(s)	1343, 1457, 1612, 1639, 1801, 1804
BseMI	GCAATG	1 cut(s)	481
BseMII	CTCAG	7 cut(s)	135, 324, 336, 645, 677, 866, 1329
BseNI	ACTGG	4 cut(s)	191, 755, 974, 1188
BseSI	GKGCMC	2 cut(s)	198, 1755
BseXI	GCAGC	8 cut(s)	70, 208, 259, 262, 313, 1191, 1351, 1792
BseYI	CCCAGC	1 cut(s)	406
BsgI	GTGCAG	2 cut(s)	207, 837
BshFI	GGCC	5 cut(s)	5, 171, 323, 405, 499
BshTI	ACCGGT	1 cut(s)	8
BsiHKAI	GWGCWC	3 cut(s)	198, 1261, 1506
BsiSI	CCGG	6 cut(s)	9, 25, 123, 138, 459, 528
BslFI	GGGAC	1 cut(s)	1493
BslI	CCNNNNNNNGG	6 cut(s)	1343, 1457, 1612, 1639, 1801, 1804
BsmAI	GTCTC	4 cut(s)	523, 1255, 1318, 1565
BsmBI	CGTCTC	1 cut(s)	1255
BsmFI	GGGAC	1 cut(s)	1493
BsnI	GGCC	5 cut(s)	5, 171, 323, 405, 499
Bso31I	GGTCTC	1 cut(s)	1565
Bsp1286I	GDGCHC	5 cut(s)	198, 1048, 1261, 1506, 1755
Bsp1407I	TGTACA	1 cut(s)	549
Bsp143I	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
Bsp1720I	GCTNAGC	1 cut(s)	357
Bsp19I	CCATGG	2 cut(s)	1237, 1799
BspACI	CCGC	6 cut(s)	99, 646, 699, 1020, 1278, 1792
BspANI	GGCC	5 cut(s)	5, 171, 323, 405, 499
BspCNI	CTCAG	7 cut(s)	136, 325, 337, 644, 676, 867, 1328
BspLI	GGNNCC	3 cut(s)	1047, 1092, 1630
BspPI	GGATC	3 cut(s)	511, 1475, 1650
BspTNI	GGTCTC	1 cut(s)	1565
BsrDI	GCAATG	1 cut(s)	481
BsrFI	RCCGGY	1 cut(s)	8
BsrGI	TGTACA	1 cut(s)	549
BsrI	ACTGG	4 cut(s)	191, 755, 974, 1188
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	4 cut(s)	616, 1237, 1451, 1799
BssMI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
BssSI	CACGAG	2 cut(s)	378, 564
BssT1I	CCWWGG	2 cut(s)	1237, 1799
Bst2BI	CACGAG	2 cut(s)	378, 564
Bst2UI	CCWGG	4 cut(s)	173, 501, 618, 1452
Bst4CI	ACNGT	5 cut(s)	114, 415, 559, 1196, 1515
Bst6I	CTCTTC	2 cut(s)	951, 1763
BstAPI	GCANNNNNTGC	1 cut(s)	218
BstAUI	TGTACA	1 cut(s)	549
BstC8I	GCNNGC	7 cut(s)	77, 488, 823, 1288, 1522, 1654, 1728
BstDEI	CTNAG	8 cut(s)	144, 333, 345, 357, 631, 663, 875, 1315
BstDSI	CCRYGG	2 cut(s)	1237, 1799
BstF5I	GGATG	6 cut(s)	226, 540, 886, 1387, 1621, 1813
BstH2I	RGCGCY	1 cut(s)	828
BstHHI	GCGC	2 cut(s)	75, 827
BstKTI	GATC	5 cut(s)	369, 506, 1012, 1470, 1645
BstMAI	GTCTC	4 cut(s)	523, 1255, 1318, 1565
BstMBI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
BstMWI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
BstNI	CCWGG	4 cut(s)	173, 501, 618, 1452
BstNSI	RCATGY	2 cut(s)	79, 821
BstPAI	GACNNNNGTC	1 cut(s)	1239
BstSCI	CCNGG	6 cut(s)	23, 136, 171, 499, 616, 1450
BstSFI	CTRYAG	2 cut(s)	262, 558
BstSLI	GKGCMC	2 cut(s)	198, 1755
BstV1I	GCAGC	8 cut(s)	70, 208, 259, 262, 313, 1191, 1351, 1792
BstV2I	GAAGAC	1 cut(s)	1110
BstX2I	RGATCY	3 cut(s)	503, 1009, 1642
BstYI	RGATCY	3 cut(s)	503, 1009, 1642
Bsu36I	CCTNAGG	2 cut(s)	663, 875
BsuRI	GGCC	5 cut(s)	5, 171, 323, 405, 499
BtgI	CCRYGG	2 cut(s)	1237, 1799
BtsCI	GGATG	6 cut(s)	226, 540, 886, 1387, 1621, 1813
BtsI	GCAGTG	3 cut(s)	231, 453, 1663
BtsIMutI	CAGTG	6 cut(s)	198, 231, 453, 762, 981, 1663
Cac8I	GCNNGC	7 cut(s)	77, 488, 823, 1288, 1522, 1654, 1728
CaiI	CAGNNNCTG	2 cut(s)	413, 1194
CfoI	GCGC	2 cut(s)	75, 827
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
CseI	GACGC	2 cut(s)	362, 1237
Csp6I	GTAC	5 cut(s)	550, 683, 806, 1197, 1546
CspAI	ACCGGT	1 cut(s)	8
CspCI	CAANNNNNGTGG	2 cut(s)	511, 546
CviQI	GTAC	5 cut(s)	550, 683, 806, 1197, 1546
DdeI	CTNAG	8 cut(s)	144, 333, 345, 357, 631, 663, 875, 1315
DpnI	GATC	5 cut(s)	368, 505, 1011, 1469, 1644
DpnII	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
DraI	TTTAAA	1 cut(s)	732
DraIII	CACNNNGTG	1 cut(s)	1824
EaeI	YGGCCR	3 cut(s)	3, 169, 321
Eam1104I	CTCTTC	2 cut(s)	951, 1763
EarI	CTCTTC	2 cut(s)	951, 1763
Ecl136II	GAGCTC	1 cut(s)	1504
Eco130I	CCWWGG	2 cut(s)	1237, 1799
Eco147I	AGGCCT	1 cut(s)	499
Eco24I	GRGCYC	2 cut(s)	1048, 1506
Eco31I	GGTCTC	1 cut(s)	1565
Eco47I	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
Eco53kI	GAGCTC	1 cut(s)	1504
Eco57I	CTGAAG	1 cut(s)	1026
Eco81I	CCTNAGG	2 cut(s)	663, 875
EcoICRI	GAGCTC	1 cut(s)	1504
EcoO109I	RGGNCCY	1 cut(s)	1294
EcoRII	CCWGG	4 cut(s)	171, 499, 616, 1450
EcoT14I	CCWWGG	2 cut(s)	1237, 1799
EcoT22I	ATGCAT	1 cut(s)	1387
EcoT38I	GRGCYC	2 cut(s)	1048, 1506
ErhI	CCWWGG	2 cut(s)	1237, 1799
Esp3I	CGTCTC	1 cut(s)	1255
FaqI	GGGAC	1 cut(s)	1493
FauI	CCCGC	2 cut(s)	92, 692
FauNDI	CATATG	3 cut(s)	373, 907, 1387
Fnu4HI	GCNGC	8 cut(s)	59, 222, 273, 276, 302, 1180, 1365, 1781
FokI	GGATG	6 cut(s)	213, 547, 893, 1394, 1628, 1820
FriOI	GRGCYC	2 cut(s)	1048, 1506
Fsp4HI	GCNGC	8 cut(s)	59, 222, 273, 276, 302, 1180, 1365, 1781
FspBI	CTAG	3 cut(s)	719, 1281, 1298
GlaI	GCGC	2 cut(s)	74, 826
GluI	GCNGC	8 cut(s)	59, 222, 273, 276, 302, 1180, 1365, 1781
GsaI	CCCAGC	1 cut(s)	410
GsuI	CTGGAG	2 cut(s)	453, 906
HaeII	RGCGCY	1 cut(s)	828
HaeIII	GGCC	5 cut(s)	5, 171, 323, 405, 499
HapII	CCGG	6 cut(s)	9, 25, 123, 138, 459, 528
HgaI	GACGC	2 cut(s)	362, 1237
HhaI	GCGC	2 cut(s)	75, 827
Hin6I	GCGC	2 cut(s)	73, 825
HinP1I	GCGC	2 cut(s)	73, 825
HincII	GTYRAC	1 cut(s)	748
HindII	GTYRAC	1 cut(s)	748
HindIII	AAGCTT	3 cut(s)	36, 726, 912
HinfI	GANTC	7 cut(s)	126, 315, 347, 423, 848, 1396, 1440
HpaI	GTTAAC	1 cut(s)	748
HpaII	CCGG	6 cut(s)	9, 25, 123, 138, 459, 528
HphI	GGTGA	5 cut(s)	461, 617, 851, 1097, 1160
Hpy166II	GTNNAC	3 cut(s)	196, 748, 1337
Hpy188I	TCNGA	7 cut(s)	156, 314, 352, 634, 802, 1075, 1710
Hpy188III	TCNNGA	2 cut(s)	1471, 1606
Hpy8I	GTNNAC	3 cut(s)	196, 748, 1337
HpyAV	CCTTC	4 cut(s)	581, 973, 1193, 1545
HpyCH4III	ACNGT	5 cut(s)	114, 415, 559, 1196, 1515
HpyCH4IV	ACGT	2 cut(s)	938, 1732
HpyCH4V	TGCA	9 cut(s)	196, 224, 377, 486, 785, 854, 971, 1385, 1520
HpyF10VI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
HpyF3I	CTNAG	8 cut(s)	144, 333, 345, 357, 631, 663, 875, 1315
HpySE526I	ACGT	2 cut(s)	938, 1732
HspAI	GCGC	2 cut(s)	73, 825
KspAI	GTTAAC	1 cut(s)	748
Kzo9I	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
LmnI	GCTCC	5 cut(s)	66, 434, 887, 1051, 1509
Lsp1109I	GCAGC	8 cut(s)	70, 208, 259, 262, 313, 1191, 1351, 1792
LweI	GCATC	4 cut(s)	221, 871, 1351, 1372
MaeI	CTAG	3 cut(s)	719, 1281, 1298
MaeII	ACGT	2 cut(s)	938, 1732
MaeIII	GTNAC	5 cut(s)	449, 568, 1190, 1273, 1460
MalI	GATC	5 cut(s)	368, 505, 1011, 1469, 1644
MboI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
MboII	GAAGA	5 cut(s)	273, 968, 1019, 1110, 1780
MfeI	CAATTG	3 cut(s)	14, 465, 1329
MflI	RGATCY	3 cut(s)	503, 1009, 1642
MhlI	GDGCHC	5 cut(s)	198, 1048, 1261, 1506, 1755
MlsI	TGGCCA	2 cut(s)	5, 323
MluNI	TGGCCA	2 cut(s)	5, 323
MlyI	GAGTC	3 cut(s)	324, 356, 857
MmeI	TCCRAC	3 cut(s)	330, 397, 1073
Mox20I	TGGCCA	2 cut(s)	5, 323
Mph1103I	ATGCAT	1 cut(s)	1387
MscI	TGGCCA	2 cut(s)	5, 323
MseI	TTAA	9 cut(s)	132, 441, 651, 669, 731, 747, 987, 1014, 1026
MslI	CAYNNNNRTG	1 cut(s)	191
Msp20I	TGGCCA	2 cut(s)	5, 323
MspI	CCGG	6 cut(s)	9, 25, 123, 138, 459, 528
MspR9I	CCNGG	6 cut(s)	25, 138, 173, 501, 618, 1452
MunI	CAATTG	3 cut(s)	14, 465, 1329
MvaI	CCWGG	4 cut(s)	173, 501, 618, 1452
MwoI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
NciI	CCSGG	2 cut(s)	25, 138
NcoI	CCATGG	2 cut(s)	1237, 1799
NdeI	CATATG	3 cut(s)	373, 907, 1387
NdeII	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
NlaIV	GGNNCC	3 cut(s)	1047, 1092, 1630
NmuCI	GTSAC	2 cut(s)	449, 1460
NsiI	ATGCAT	1 cut(s)	1387
NspI	RCATGY	2 cut(s)	79, 821
PaeI	GCATGC	1 cut(s)	79
PceI	AGGCCT	1 cut(s)	499
PfeI	GAWTC	4 cut(s)	126, 423, 1396, 1440
PflMI	CCANNNNNTGG	2 cut(s)	1343, 1457
PfoI	TCCNGGA	1 cut(s)	23
PinAI	ACCGGT	1 cut(s)	8
PkrI	GCNGC	8 cut(s)	60, 223, 274, 277, 303, 1181, 1366, 1782
PleI	GAGTC	3 cut(s)	323, 355, 856
PpsI	GAGTC	3 cut(s)	323, 355, 856
PpuMI	RGGWCCY	1 cut(s)	1294
PshAI	GACNNNNGTC	1 cut(s)	1239
PshBI	ATTAAT	1 cut(s)	132
Psp124BI	GAGCTC	1 cut(s)	1506
Psp1406I	AACGTT	1 cut(s)	938
Psp5II	RGGWCCY	1 cut(s)	1294
Psp6I	CCWGG	4 cut(s)	171, 499, 616, 1450
PspFI	CCCAGC	1 cut(s)	406
PspGI	CCWGG	4 cut(s)	171, 499, 616, 1450
PspN4I	GGNNCC	3 cut(s)	1047, 1092, 1630
PspPI	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
PspPPI	RGGWCCY	1 cut(s)	1294
PstNI	CAGNNNCTG	2 cut(s)	413, 1194
PsuI	RGATCY	3 cut(s)	503, 1009, 1642
RsaI	GTAC	5 cut(s)	551, 684, 807, 1198, 1547
RsaNI	GTAC	5 cut(s)	550, 683, 806, 1197, 1546
RseI	CAYNNNNRTG	1 cut(s)	191
SacI	GAGCTC	1 cut(s)	1506
SaqAI	TTAA	9 cut(s)	132, 441, 651, 669, 731, 747, 987, 1014, 1026
SatI	GCNGC	8 cut(s)	59, 222, 273, 276, 302, 1180, 1365, 1781
Sau3AI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
Sau96I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
SchI	GAGTC	3 cut(s)	324, 356, 857
ScrFI	CCNGG	6 cut(s)	25, 138, 173, 501, 618, 1452
SduI	GDGCHC	5 cut(s)	198, 1048, 1261, 1506, 1755
SfaNI	GCATC	4 cut(s)	221, 871, 1351, 1372
SfcI	CTRYAG	2 cut(s)	262, 558
SinI	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
SmiMI	CAYNNNNRTG	1 cut(s)	191
SmlI	CTYRAG	3 cut(s)	33, 199, 296
SmoI	CTYRAG	3 cut(s)	33, 199, 296
SphI	GCATGC	1 cut(s)	79
SseBI	AGGCCT	1 cut(s)	499
SsiI	CCGC	6 cut(s)	99, 646, 699, 1020, 1278, 1792
SspMI	CTAG	3 cut(s)	719, 1281, 1298
SstI	GAGCTC	1 cut(s)	1506
StuI	AGGCCT	1 cut(s)	499
StyD4I	CCNGG	6 cut(s)	23, 136, 171, 499, 616, 1450
StyI	CCWWGG	2 cut(s)	1237, 1799
TaaI	ACNGT	5 cut(s)	114, 415, 559, 1196, 1515
TaiI	ACGT	2 cut(s)	941, 1735
TaqI	TCGA	2 cut(s)	1369, 1419
TatI	WGTACW	4 cut(s)	549, 682, 805, 1545
TfiI	GAWTC	4 cut(s)	126, 423, 1396, 1440
Tru1I	TTAA	9 cut(s)	132, 441, 651, 669, 731, 747, 987, 1014, 1026
Tru9I	TTAA	9 cut(s)	132, 441, 651, 669, 731, 747, 987, 1014, 1026
TscAI	CASTG	6 cut(s)	198, 238, 460, 762, 981, 1663
TseFI	GTSAC	2 cut(s)	449, 1460
TseI	GCWGC	8 cut(s)	58, 221, 272, 275, 301, 1179, 1364, 1780
Tsp45I	GTSAC	2 cut(s)	449, 1460
TspDTI	ATGAA	6 cut(s)	470, 558, 600, 894, 924, 1442
TspGWI	ACGGA	2 cut(s)	591, 1433
TspRI	CASTG	6 cut(s)	198, 238, 460, 762, 981, 1663
Van91I	CCANNNNNTGG	2 cut(s)	1343, 1457
VneI	GTGCAC	1 cut(s)	194
VpaK11BI	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
VspI	ATTAAT	1 cut(s)	132
XapI	RAATTY	1 cut(s)	983
XceI	RCATGY	2 cut(s)	79, 821
XcmI	CCANNNNNNNNNTGG	2 cut(s)	316, 762
XspI	CTAG	3 cut(s)	719, 1281, 1298
Zsp2I	ATGCAT	1 cut(s)	1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.