Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008568

Physical Location & Seq

Genomic Coordinates Reverse (-)

12028027 .. 12033283

Gene Structure 5257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1153500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGGCCACGGGACAACTTTTTTCGCGCACCACTCAGGCGCTGTTCTACAACTACAAGCAGCTCCCAGTCCAGCGCATGCTCGATTTTGACTTCCTTTGCGGGAGGGAGACACCATCAGTTGCTGGAATCATTAACCCCGGTTCTGAGGGATTTCAGAAGCTATTTTTCGGTCAAGAGGAAATCGCCATACCAGTGCACTCAAACATTGAAGCATCATGCGCTGCACATCCTACTGCTGATGTCTTTATCAACTTTGCTTCATACAGAAGTGCTGCCGCTTCTTCCATGGCTGCTCTGAAGCAACCAACCATTAGAGTTGTGGCAATCATTGCTGAAGGTGTACCTGAGTCAGACACTAAGCAATTGATCGCATATGCACGGGCAAACAACAAGGTTGTCATTGGCCCAGCTACTGTTGGAGGAATTCAAGCTGGAGCTTTTAAGATTGGTGACACTGCTGGAACAATTGATAACATTATTCACTGCAAGCTATACAGGCCTGGATCTGTTGGTTTTGTCTCCAAATCTGGTGGGATGTCTAATGAGTTATACAATACTGTAGCTCGTGTAACAGATGGGCTTTTTGAAGGTATTGCTATTGGAGGTGATGTGTTCCCAGGGTCTACTCTTTCTGACCATGTTCTGCGGTATAACAATATCCCCCAGGTCAAACTTATTGTTGTACTTGGGGAACTTGGTGGGAGAGACGAGTATTCTCTAGTTGAAGCTTTGAAACAGGGCAAAGTTACCAAACCAGTGGTTGCTTGGGTTAGTGGGACTTGTGCAAGGCTTTTCAAATCCGAAGTGCAATTTGGTCATGCTGGCGCAAAAAGTGGTGGTGAGATGGAGTCTGCACAAGCAAAGAATCAAGCCCTGAGGGATGCTGGTGCTGTTGTTCCCACTTCATATGAAGCTTTGGAAATTGCTATCAAGGAAACATTTGAAAAACTGGTTGAAGAAGGCAAGATTGCACCAGTCAAGGAATTCAAGCCTCCACAAATACCTGAAGATCTCAACTCTGCGATTAAGAGCGGAAAAGTTCGTGCTCCAACTCATATCATATCCACAATCTCTGATGACAGAGGTGAGGAACCATGTTATGCTGGTGTACCTATGTCTTCCATTGTTGAGCAGGGTTATGGTGTCGGTGACGTTATCTCTCTTTTGTGGTTCAAGCGTAGCCTTCCCAGTTACTGTACTAAATTTATTGAGATTTGCATCATGTTATGTGCCGACCATGGTCCTTGCGTCTCTGGTGCACACAACTCAATAGTAACTGCTAGGGCTGGCAAGGACCTTGTTTCCTGCCTTGTTTCAGGTTTGCTCACAATTGGACCACGATTTGGTGGTGCCGTTGATGATGCTGCTCGATATTTCAAGGATGCATATGACAGGAATCTTACACCTTATGAGTTCGTTGAAAGTATGAAGAAGAAGGGCATTCGTGTGCCAGGGATTGGGCATAGGATCAAGAGAGGGGACAACAGAGATAAGAGAGTAGAGCTTCTACAAGCGTTTGCACGCACACATTTCCCTTCTGTCAAGTACATGGAATATGCCGTTCAAGTTGAAACCTACACACTCTCGAAGGCAAACAACCTGGTCCTCAATGTTGATGGTGCAATTGGAACCCTGTTCTTGGATCTTCTTGCTGGCAGTGGAATGTTCGCCAAACCAGAGATTGACGAGATTGTTGAGATTGGATATCTGAATGGGCTATTCGTGCTGGCACGTTCTATTGGTTTGATTGGGCACACATTCGACCAGAAGAGGTTGAAGCAGCCCCTATACCGCCACCCATGGGAGGACGTTCTTTACACCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016020 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

Citrate_synt CoA_binding Ligase_CoA

Protein Analysis

609

Amino Acids

65.9

Weight (kDa)

7.56

Isoelectric Point (pI)

32.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ligase_CoA	PF00549	173 - 298	9.5e-19	CoA-ligase
Citrate_synt	PF00285	400 - 597	2.7e-17	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008568)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06650 AT3G06650 AT5G49460 AT5G49460
fragaria_vesca	FvH4_4g19740 FvH4_4g19740 FvH4_4g19740 FvH4_4g19740
malus_domestica	MD13G1153500.v1.1 MD16G1154000.v1.1
prunus_persica	Prupe.1G117200_v2.0.a1 Prupe.1G117200_v2.0.a1
pyrus_communis	pycom13g13120 pycom16g13050
rosa_chinensis	RchiOBHm_Chr4g0425291
rosa_laevigata	RLG00000007382
rosa_multiflora	Rmu_sc0009125.1_g000003
rosa_roxburghii	Rroxscaffold_5G00367290
rosa_rugosa	Rorug04G0202100 Rorug04G0202200 Rorug04G0202300
rosa_samantha	Rh4CG276200
rosa_wichuraiana	Rw4G022420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1349
AccB7I	CCANNNNNTGG	2 cut(s)	1343, 1457
AccBSI	CCGCTC	1 cut(s)	1032
AccI	GTMKAC	1 cut(s)	623
AccII	CGCG	1 cut(s)	25
AciI	CCGC	5 cut(s)	99, 276, 646, 1032, 1792
AclWI	GGATC	3 cut(s)	511, 1475, 1650
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	423, 983, 1202
AcuI	CTGAAG	3 cut(s)	317, 354, 1026
AdeI	CACNNNGTG	1 cut(s)	1824
AfaI	GTAC	5 cut(s)	342, 684, 1110, 1198, 1547
AfiI	CCNNNNNNNGG	5 cut(s)	1343, 1457, 1639, 1801, 1804
AjnI	CCWGG	5 cut(s)	499, 616, 663, 1450, 1599
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
Alw21I	GWGCWC	3 cut(s)	198, 1048, 1261
Alw26I	GTCTC	4 cut(s)	101, 523, 699, 1255
Alw44I	GTGCAC	2 cut(s)	194, 1257
AlwI	GGATC	3 cut(s)	511, 1475, 1650
AlwNI	CAGNNNCTG	4 cut(s)	40, 122, 413, 1194
AoxI	GGCC	3 cut(s)	3, 403, 497
ApaLI	GTGCAC	2 cut(s)	194, 1257
ApeKI	GCWGC	6 cut(s)	58, 221, 272, 290, 1364, 1780
ApoI	RAATTY	3 cut(s)	423, 983, 1202
AspLEI	GCGC	5 cut(s)	27, 40, 75, 221, 827
AspS9I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
AsuC2I	CCSGG	1 cut(s)	138
AsuHPI	GGTGA	5 cut(s)	461, 617, 851, 1097, 1160
AvaII	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
AxyI	CCTNAGG	1 cut(s)	875
BaeGI	GKGCMC	3 cut(s)	198, 1261, 1755
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	1349
BauI	CACGAG	1 cut(s)	564
BbsI	GAAGAC	1 cut(s)	1110
Bbv12I	GWGCWC	3 cut(s)	198, 1048, 1261
BbvI	GCAGC	6 cut(s)	70, 208, 259, 277, 1351, 1792
BccI	CCATC	4 cut(s)	121, 569, 838, 1610
BceAI	ACGGC	2 cut(s)	1337, 1544
BciT130I	CCWGG	5 cut(s)	501, 618, 665, 1452, 1601
BcnI	CCSGG	1 cut(s)	138
BcoDI	GTCTC	4 cut(s)	101, 523, 699, 1255
BfaI	CTAG	2 cut(s)	719, 1281
BfmI	CTRYAG	1 cut(s)	558
BfoI	RGCGCY	1 cut(s)	41
BglII	AGATCT	1 cut(s)	1009
BisI	GCNGC	7 cut(s)	59, 222, 273, 276, 291, 1365, 1781
BlsI	GCNGC	7 cut(s)	60, 223, 274, 277, 292, 1366, 1782
Bme1390I	CCNGG	6 cut(s)	138, 501, 618, 665, 1452, 1601
Bme18I	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
BmgT120I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
BmiI	GGNNCC	3 cut(s)	1092, 1351, 1630
BmrFI	CCNGG	6 cut(s)	138, 501, 618, 665, 1452, 1601
BmrI	ACTGGG	2 cut(s)	59, 1182
BmsI	GCATC	5 cut(s)	221, 871, 1227, 1351, 1372
BmuI	ACTGGG	2 cut(s)	59, 1182
BoxI	GACNNNNGTC	1 cut(s)	1239
BpiI	GAAGAC	1 cut(s)	1110
BpmI	CTGGAG	1 cut(s)	453
BpuMI	CCSGG	1 cut(s)	138
BsaBI	GATNNNNATC	1 cut(s)	1217
BsaJI	CCNNGG	9 cut(s)	6, 136, 285, 616, 617, 663, 1237, 1451, 1799
BsaXI	ACNNNNNCTCC	2 cut(s)	594, 624
Bsc4I	CCNNNNNNNGG	5 cut(s)	1343, 1457, 1639, 1801, 1804
Bse1I	ACTGG	6 cut(s)	65, 191, 755, 954, 974, 1188
Bse21I	CCTNAGG	1 cut(s)	875
Bse3DI	GCAATG	1 cut(s)	327
Bse8I	GATNNNNATC	1 cut(s)	1217
BseBI	CCWGG	5 cut(s)	501, 618, 665, 1452, 1601
BseDI	CCNNGG	9 cut(s)	6, 136, 285, 616, 617, 663, 1237, 1451, 1799
BseGI	GGATG	4 cut(s)	226, 540, 886, 1387
BseJI	GATNNNNATC	1 cut(s)	1217
BseLI	CCNNNNNNNGG	5 cut(s)	1343, 1457, 1639, 1801, 1804
BseMI	GCAATG	1 cut(s)	327
BseMII	CTCAG	4 cut(s)	47, 135, 336, 866
BseNI	ACTGG	6 cut(s)	65, 191, 755, 954, 974, 1188
BseSI	GKGCMC	3 cut(s)	198, 1261, 1755
BseXI	GCAGC	6 cut(s)	70, 208, 259, 277, 1351, 1792
BseYI	CCCAGC	1 cut(s)	406
BsgI	GTGCAG	2 cut(s)	207, 837
Bsh1236I	CGCG	1 cut(s)	25
BshFI	GGCC	3 cut(s)	5, 405, 499
BshNI	GGYRCC	1 cut(s)	1349
BsiHKAI	GWGCWC	3 cut(s)	198, 1048, 1261
BsiSI	CCGG	1 cut(s)	138
BslFI	GGGAC	3 cut(s)	24, 790, 1493
BslI	CCNNNNNNNGG	5 cut(s)	1343, 1457, 1639, 1801, 1804
BsmAI	GTCTC	4 cut(s)	101, 523, 699, 1255
BsmBI	CGTCTC	2 cut(s)	699, 1255
BsmFI	GGGAC	3 cut(s)	24, 790, 1493
BsmI	GAATGC	1 cut(s)	1440
BsnI	GGCC	3 cut(s)	5, 405, 499
Bsp1286I	GDGCHC	4 cut(s)	198, 1048, 1261, 1755
Bsp143I	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
Bsp19I	CCATGG	3 cut(s)	285, 1237, 1799
BspACI	CCGC	5 cut(s)	99, 276, 646, 1032, 1792
BspANI	GGCC	3 cut(s)	5, 405, 499
BspCNI	CTCAG	4 cut(s)	46, 136, 337, 867
BspFNI	CGCG	1 cut(s)	25
BspLI	GGNNCC	3 cut(s)	1092, 1351, 1630
BspPI	GGATC	3 cut(s)	511, 1475, 1650
BspT107I	GGYRCC	1 cut(s)	1349
BsrBI	CCGCTC	1 cut(s)	1032
BsrDI	GCAATG	1 cut(s)	327
BsrI	ACTGG	6 cut(s)	65, 191, 755, 954, 974, 1188
BssECI	CCNNGG	9 cut(s)	6, 136, 285, 616, 617, 663, 1237, 1451, 1799
BssMI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
BssSI	CACGAG	1 cut(s)	564
BssT1I	CCWWGG	3 cut(s)	285, 1237, 1799
Bst2BI	CACGAG	1 cut(s)	564
Bst2UI	CCWGG	5 cut(s)	501, 618, 665, 1452, 1601
Bst4CI	ACNGT	3 cut(s)	415, 559, 1196
Bst6I	CTCTTC	1 cut(s)	1763
BstAPI	GCANNNNNTGC	1 cut(s)	329
BstC8I	GCNNGC	7 cut(s)	77, 488, 823, 1288, 1522, 1654, 1728
BstDEI	CTNAG	5 cut(s)	33, 144, 345, 357, 875
BstDSI	CCRYGG	4 cut(s)	6, 285, 1237, 1799
BstF5I	GGATG	4 cut(s)	226, 540, 886, 1387
BstFNI	CGCG	1 cut(s)	25
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	5 cut(s)	27, 40, 75, 221, 827
BstKTI	GATC	5 cut(s)	369, 506, 1012, 1470, 1645
BstMAI	GTCTC	4 cut(s)	101, 523, 699, 1255
BstMBI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
BstMWI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
BstNI	CCWGG	5 cut(s)	501, 618, 665, 1452, 1601
BstNSI	RCATGY	1 cut(s)	79
BstPAI	GACNNNNGTC	1 cut(s)	1239
BstSCI	CCNGG	6 cut(s)	136, 499, 616, 663, 1450, 1599
BstSFI	CTRYAG	1 cut(s)	558
BstSLI	GKGCMC	3 cut(s)	198, 1261, 1755
BstUI	CGCG	1 cut(s)	25
BstV1I	GCAGC	6 cut(s)	70, 208, 259, 277, 1351, 1792
BstV2I	GAAGAC	1 cut(s)	1110
BstX2I	RGATCY	3 cut(s)	503, 1009, 1642
BstXI	CCANNNNNNTGG	1 cut(s)	757
BstYI	RGATCY	3 cut(s)	503, 1009, 1642
Bsu36I	CCTNAGG	1 cut(s)	875
BsuRI	GGCC	3 cut(s)	5, 405, 499
BtgI	CCRYGG	4 cut(s)	6, 285, 1237, 1799
BtsCI	GGATG	4 cut(s)	226, 540, 886, 1387
BtsI	GCAGTG	3 cut(s)	453, 481, 1663
BtsIMutI	CAGTG	5 cut(s)	198, 453, 481, 762, 1663
Cac8I	GCNNGC	7 cut(s)	77, 488, 823, 1288, 1522, 1654, 1728
CaiI	CAGNNNCTG	4 cut(s)	40, 122, 413, 1194
CfoI	GCGC	5 cut(s)	27, 40, 75, 221, 827
Cfr13I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
CseI	GACGC	1 cut(s)	1237
CsiI	ACCWGGT	1 cut(s)	1599
Csp6I	GTAC	5 cut(s)	341, 683, 1109, 1197, 1546
CspCI	CAANNNNNGTGG	2 cut(s)	511, 546
CviQI	GTAC	5 cut(s)	341, 683, 1109, 1197, 1546
DdeI	CTNAG	5 cut(s)	33, 144, 345, 357, 875
DpnI	GATC	5 cut(s)	368, 505, 1011, 1469, 1644
DpnII	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
DraIII	CACNNNGTG	1 cut(s)	1824
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	1763
EarI	CTCTTC	1 cut(s)	1763
Eco130I	CCWWGG	3 cut(s)	285, 1237, 1799
Eco147I	AGGCCT	1 cut(s)	499
Eco32I	GATATC	1 cut(s)	1706
Eco47I	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
Eco57I	CTGAAG	3 cut(s)	317, 354, 1026
Eco81I	CCTNAGG	1 cut(s)	875
EcoO109I	RGGNCCY	1 cut(s)	1294
EcoRI	GAATTC	2 cut(s)	423, 983
EcoRII	CCWGG	5 cut(s)	499, 616, 663, 1450, 1599
EcoRV	GATATC	1 cut(s)	1706
EcoT14I	CCWWGG	3 cut(s)	285, 1237, 1799
EcoT22I	ATGCAT	1 cut(s)	1387
ErhI	CCWWGG	3 cut(s)	285, 1237, 1799
Esp3I	CGTCTC	2 cut(s)	699, 1255
FaqI	GGGAC	3 cut(s)	24, 790, 1493
FauI	CCCGC	1 cut(s)	92
FauNDI	CATATG	3 cut(s)	373, 907, 1387
FblI	GTMKAC	1 cut(s)	623
Fnu4HI	GCNGC	7 cut(s)	59, 222, 273, 276, 291, 1365, 1781
FokI	GGATG	4 cut(s)	213, 547, 893, 1394
Fsp4HI	GCNGC	7 cut(s)	59, 222, 273, 276, 291, 1365, 1781
FspBI	CTAG	2 cut(s)	719, 1281
GlaI	GCGC	5 cut(s)	26, 39, 74, 220, 826
GluI	GCNGC	7 cut(s)	59, 222, 273, 276, 291, 1365, 1781
GsaI	CCCAGC	1 cut(s)	410
GsuI	CTGGAG	1 cut(s)	453
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	3 cut(s)	5, 405, 499
HapII	CCGG	1 cut(s)	138
HgaI	GACGC	1 cut(s)	1237
HhaI	GCGC	5 cut(s)	27, 40, 75, 221, 827
Hin6I	GCGC	5 cut(s)	25, 38, 73, 219, 825
HinP1I	GCGC	5 cut(s)	25, 38, 73, 219, 825
HindIII	AAGCTT	2 cut(s)	726, 912
HinfI	GANTC	5 cut(s)	126, 347, 848, 865, 1396
HpaII	CCGG	1 cut(s)	138
HphI	GGTGA	5 cut(s)	461, 617, 851, 1097, 1160
Hpy166II	GTNNAC	5 cut(s)	196, 341, 624, 1109, 1259
Hpy188I	TCNGA	8 cut(s)	145, 156, 297, 352, 634, 802, 1075, 1710
Hpy188III	TCNNGA	3 cut(s)	173, 1471, 1585
Hpy8I	GTNNAC	5 cut(s)	196, 341, 624, 1109, 1259
HpyAV	CCTTC	7 cut(s)	329, 581, 953, 1193, 1429, 1545, 1582
HpyCH4III	ACNGT	3 cut(s)	415, 559, 1196
HpyCH4IV	ACGT	3 cut(s)	1152, 1732, 1809
HpyF10VI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
HpyF3I	CTNAG	5 cut(s)	33, 144, 345, 357, 875
HpySE526I	ACGT	3 cut(s)	1152, 1732, 1809
HspAI	GCGC	5 cut(s)	25, 38, 73, 219, 825
Kzo9I	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
LmnI	GCTCC	3 cut(s)	66, 434, 1051
Lsp1109I	GCAGC	6 cut(s)	70, 208, 259, 277, 1351, 1792
LweI	GCATC	5 cut(s)	221, 871, 1227, 1351, 1372
MabI	ACCWGGT	1 cut(s)	1599
MaeI	CTAG	2 cut(s)	719, 1281
MaeII	ACGT	3 cut(s)	1152, 1732, 1809
MaeIII	GTNAC	6 cut(s)	449, 568, 745, 1148, 1190, 1273
MalI	GATC	5 cut(s)	368, 505, 1011, 1469, 1644
MbiI	CCGCTC	1 cut(s)	1032
MboI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
MboII	GAAGA	8 cut(s)	273, 968, 1019, 1110, 1441, 1444, 1637, 1780
MfeI	CAATTG	4 cut(s)	362, 465, 1329, 1623
MflI	RGATCY	3 cut(s)	503, 1009, 1642
MhlI	GDGCHC	4 cut(s)	198, 1048, 1261, 1755
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	9 cut(s)	362, 423, 465, 809, 921, 983, 1202, 1329, 1623
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	356, 857
MmeI	TCCRAC	2 cut(s)	397, 1073
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	1387
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	132, 441, 1026
MslI	CAYNNNNRTG	2 cut(s)	191, 1445
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	1 cut(s)	138
MspR9I	CCNGG	6 cut(s)	138, 501, 618, 665, 1452, 1601
MunI	CAATTG	4 cut(s)	362, 465, 1329, 1623
Mva1269I	GAATGC	1 cut(s)	1440
MvaI	CCWGG	5 cut(s)	501, 618, 665, 1452, 1601
MvnI	CGCG	1 cut(s)	25
MwoI	GCNNNNNNNGC	4 cut(s)	218, 329, 496, 1723
NciI	CCSGG	1 cut(s)	138
NcoI	CCATGG	3 cut(s)	285, 1237, 1799
NdeI	CATATG	3 cut(s)	373, 907, 1387
NdeII	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
NlaIV	GGNNCC	3 cut(s)	1092, 1351, 1630
NmuCI	GTSAC	2 cut(s)	449, 1148
NsiI	ATGCAT	1 cut(s)	1387
NspI	RCATGY	1 cut(s)	79
PaeI	GCATGC	1 cut(s)	79
PasI	CCCWGGG	1 cut(s)	617
PceI	AGGCCT	1 cut(s)	499
PctI	GAATGC	1 cut(s)	1440
PfeI	GAWTC	3 cut(s)	126, 865, 1396
PflMI	CCANNNNNTGG	2 cut(s)	1343, 1457
PkrI	GCNGC	7 cut(s)	60, 223, 274, 277, 292, 1366, 1782
PleI	GAGTC	2 cut(s)	355, 856
PpsI	GAGTC	2 cut(s)	355, 856
PpuMI	RGGWCCY	1 cut(s)	1294
PshAI	GACNNNNGTC	1 cut(s)	1239
Psp5II	RGGWCCY	1 cut(s)	1294
Psp6I	CCWGG	5 cut(s)	499, 616, 663, 1450, 1599
PspFI	CCCAGC	1 cut(s)	406
PspGI	CCWGG	5 cut(s)	499, 616, 663, 1450, 1599
PspN4I	GGNNCC	3 cut(s)	1092, 1351, 1630
PspPI	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
PspPPI	RGGWCCY	1 cut(s)	1294
PstNI	CAGNNNCTG	4 cut(s)	40, 122, 413, 1194
PsuI	RGATCY	3 cut(s)	503, 1009, 1642
RsaI	GTAC	5 cut(s)	342, 684, 1110, 1198, 1547
RsaNI	GTAC	5 cut(s)	341, 683, 1109, 1197, 1546
RseI	CAYNNNNRTG	2 cut(s)	191, 1445
SaqAI	TTAA	3 cut(s)	132, 441, 1026
SatI	GCNGC	7 cut(s)	59, 222, 273, 276, 291, 1365, 1781
Sau3AI	GATC	5 cut(s)	366, 503, 1009, 1467, 1642
Sau96I	GGNCC	5 cut(s)	404, 1241, 1294, 1334, 1603
SchI	GAGTC	2 cut(s)	356, 857
ScrFI	CCNGG	6 cut(s)	138, 501, 618, 665, 1452, 1601
SduI	GDGCHC	4 cut(s)	198, 1048, 1261, 1755
SexAI	ACCWGGT	1 cut(s)	1599
SfaNI	GCATC	5 cut(s)	221, 871, 1227, 1351, 1372
SfcI	CTRYAG	1 cut(s)	558
SinI	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
SmiMI	CAYNNNNRTG	2 cut(s)	191, 1445
SphI	GCATGC	1 cut(s)	79
Sse9I	AATT	9 cut(s)	362, 423, 465, 809, 921, 983, 1202, 1329, 1623
SseBI	AGGCCT	1 cut(s)	499
SsiI	CCGC	5 cut(s)	99, 276, 646, 1032, 1792
SspMI	CTAG	2 cut(s)	719, 1281
StuI	AGGCCT	1 cut(s)	499
StyD4I	CCNGG	6 cut(s)	136, 499, 616, 663, 1450, 1599
StyI	CCWWGG	3 cut(s)	285, 1237, 1799
TaaI	ACNGT	3 cut(s)	415, 559, 1196
TaiI	ACGT	3 cut(s)	1155, 1735, 1812
TaqI	TCGA	4 cut(s)	81, 1369, 1586, 1761
TaqII	GACCGA	1 cut(s)	158
TasI	AATT	9 cut(s)	362, 423, 465, 809, 921, 983, 1202, 1329, 1623
TatI	WGTACW	3 cut(s)	682, 1196, 1545
TauI	GCSGC	1 cut(s)	278
TfiI	GAWTC	3 cut(s)	126, 865, 1396
Tru1I	TTAA	3 cut(s)	132, 441, 1026
Tru9I	TTAA	3 cut(s)	132, 441, 1026
TscAI	CASTG	5 cut(s)	198, 460, 488, 762, 1663
TseFI	GTSAC	2 cut(s)	449, 1148
TseI	GCWGC	6 cut(s)	58, 221, 272, 290, 1364, 1780
Tsp45I	GTSAC	2 cut(s)	449, 1148
TspDTI	ATGAA	4 cut(s)	249, 894, 924, 1442
TspRI	CASTG	5 cut(s)	198, 460, 488, 762, 1663
Van91I	CCANNNNNTGG	2 cut(s)	1343, 1457
VneI	GTGCAC	2 cut(s)	194, 1257
VpaK11BI	GGWCC	4 cut(s)	1241, 1294, 1334, 1603
XapI	RAATTY	3 cut(s)	423, 983, 1202
XceI	RCATGY	1 cut(s)	79
XcmI	CCANNNNNNNNNTGG	2 cut(s)	316, 762
XmiI	GTMKAC	1 cut(s)	623
XspI	CTAG	2 cut(s)	719, 1281
Zsp2I	ATGCAT	1 cut(s)	1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.