AT3G10700

Galactokinase galactose-binding signature

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003653
Physical Location & Seq
Reverse (-)
3346509 .. 3351170
4662 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10700.2

Sequence Viewer

Length: 1275 bp
ATGTCTTGGCCTACGGATTCTGAGTTAAATTCCATAAAGGAGGCAGTGGCTCAGATGAGTGGAAGAGATAAAGGAGAAGTTCGAGTGGTGGTCGCTCCTTATCGTATATGTCCTTTAGGAGCTCACATTGATCACCAGGGTGGAACTGTATCAGCTATGACGATTAATAAAGGGATCCTTCTTGGTTTTGTTCCATCGGGTGATACTCAGGTCCAGTTGCGCTCTGCACAATTTGAAGGAGAAGTATGTTTCAGAGTAGATGAAATCCAGCACCCAATAGGCCTAGCAAACAAGAATGGTGCAAGCACGCCATCTCCATCGAAGGAAAAAAGTATCTGGGGTACTTATGCCAGAGGAGCAGTTTATGCGTTACAGAGCAGCAAAAAGAATCTCAAACAGGGCATTATTGGTTACCTCAGTGGCTCAAATGGACTAGATAGCTCCGGGCTTAGCTCATCAGCTGCTGTTGGTGTGGCATACCTGCTAGCTCTAGAGAATGCAAACGAATTGACTGTATCCCCAACAGAAAATATCGAATATGACAGGCTTATTGAGAATGGGTATCTGGGTCTGCGGAATGGAATTTTGGATCAATCAGCTATTTTGCTTTCGAATTATGGGTGTCTAACATACATGGACTGCAAGACTTTGGACCACGAGCTTGTACAGGCTCCTGAACTGGAGAAACCGTTCAGGATATTGTTAGCATTCTCAGGCTTGAGGCAGGCGTTGACCACCAACCCAGGATATAATCTGCGAGTTTCTGAGTGTCAAGAGGCAGCAAAAGTTCTTTTGACTGCATCTGGGAACAGTGAGCTGGAACCTACGTTGTGCAATGTTGAGCATGCGGTCTATGAAGCTCACAAGCATGAGCTGAAACCGGTTTTAGCTAAAAGAGCAGAGCATTATTTCTCGGAGAACATGCGAGTTATCAAAGGACGGGAAGCCTGGGCTTCAGGGAATCTTGAAGAATTTGGAAAGCTAATTTCAGCATCCGGCTTGAGTTCCATTGAGAATTACGAATGCGGTGCGGAGCCACTGATCCAGCTATACAAGATTCTTCTGAAGGCTCCTGGTGTATATGGAGCTAGATTCAGCGGTGCAGGTTTCAGGGGATGTTGTCTAGCCTTTGTAGATGCAGAAAAAGCTGAGGCAGCTGCTTCATATGTGAAGGATGAATATGAAAAGGCCCAACCCGAGTTTGCTAACAATCTAAATGGAGGAAAACCTGTTCTCATCTGTGAAGCAGGTGACGCTGCTCGTGTTCTTCTCTGA

Protein Analysis

424

Amino Acids

45.73

Weight (kDa)

5.54

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 142 - 204 1.8e-08 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1238
Acc36I ACCTGC 3 cut(s) 489, 1094, 1238
AciI CCGC 5 cut(s) 574, 848, 1026, 1031, 1098
AclWI GGATC 4 cut(s) 169, 182, 597, 1036
AcsI RAATTY 3 cut(s) 28, 582, 971
AcuI CTGAAG 2 cut(s) 939, 1085
AfaI GTAC 2 cut(s) 343, 666
AgeI ACCGGT 1 cut(s) 880
AgsI TTSAA 2 cut(s) 236, 968
AjnI CCWGG 4 cut(s) 135, 742, 947, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AleI CACNNNNGTG 1 cut(s) 138
AloI GAACNNNNNNTCC 4 cut(s) 674, 706, 1215, 1247
Alw21I GWGCWC 1 cut(s) 124
AlwI GGATC 4 cut(s) 169, 182, 597, 1036
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 1196
AoxI GGCC 3 cut(s) 8, 280, 1188
ApeKI GCWGC 6 cut(s) 378, 461, 779, 1154, 1157, 1256
ApoI RAATTY 3 cut(s) 28, 582, 971
AseI ATTAAT 1 cut(s) 165
AsiGI ACCGGT 1 cut(s) 880
Asp700I GAANNNNTTC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 3 cut(s) 211, 652, 1189
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 3 cut(s) 125, 212, 1262
AsuII TTCGAA 1 cut(s) 611
AsuNHI GCTAGC 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 1196
AvaII GGWCC 2 cut(s) 211, 652
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BamHI GGATCC 1 cut(s) 174
BanII GRGCYC 1 cut(s) 124
BauI CACGAG 2 cut(s) 656, 1260
Bbv12I GWGCWC 1 cut(s) 124
BbvCI CCTCAGC 1 cut(s) 1149
BbvI GCAGC 6 cut(s) 390, 448, 791, 1144, 1166, 1243
BccI CCATC 3 cut(s) 202, 319, 325
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 4 cut(s) 137, 744, 949, 1074
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 445
BfaI CTAG 6 cut(s) 284, 434, 485, 491, 1089, 1124
BfuAI ACCTGC 3 cut(s) 489, 1094, 1238
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 6 cut(s) 379, 462, 780, 1155, 1158, 1257
BlpI GCTNAGC 1 cut(s) 449
BlsI GCNGC 6 cut(s) 380, 463, 781, 1156, 1159, 1258
Bme1390I CCNGG 5 cut(s) 137, 445, 744, 949, 1074
Bme18I GGWCC 2 cut(s) 211, 652
BmeT110I CYCGRG 1 cut(s) 1196
BmgT120I GGNCC 3 cut(s) 211, 652, 1189
BmiI GGNNCC 5 cut(s) 176, 672, 822, 1035, 1071
BmrFI CCNGG 5 cut(s) 137, 445, 744, 949, 1074
BmsI GCATC 3 cut(s) 809, 1001, 1126
BmtI GCTAGC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 1 cut(s) 1149
Bpu1102I GCTNAGC 1 cut(s) 449
Bpu14I TTCGAA 1 cut(s) 611
BpuEI CTTGAG 2 cut(s) 739, 1021
BpuMI CCSGG 1 cut(s) 445
BsaJI CCNNGG 3 cut(s) 136, 742, 948
BsaWI WCCGGW 1 cut(s) 880
BsaXI ACNNNNNCTCC 4 cut(s) 298, 328, 674, 704
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 2 cut(s) 214, 684
Bse3DI GCAATG 1 cut(s) 841
BseBI CCWGG 4 cut(s) 137, 744, 949, 1074
BseDI CCNNGG 3 cut(s) 136, 742, 948
BseGI GGATG 3 cut(s) 992, 1121, 1180
BseMI GCAATG 1 cut(s) 841
BseMII CTCAG 7 cut(s) 12, 65, 221, 430, 726, 756, 1140
BseNI ACTGG 2 cut(s) 214, 684
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 6 cut(s) 390, 448, 791, 1144, 1166, 1243
BsgI GTGCAG 2 cut(s) 210, 1122
BshFI GGCC 3 cut(s) 10, 282, 1190
BshTI ACCGGT 1 cut(s) 880
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 1196
BsiSI CCGG 3 cut(s) 444, 881, 996
BsmI GAATGC 3 cut(s) 502, 707, 1028
BsnI GGCC 3 cut(s) 10, 282, 1190
BsoBI CYCGRG 1 cut(s) 1196
Bsp119I TTCGAA 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 124
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 4 cut(s) 130, 174, 589, 1041
Bsp1720I GCTNAGC 1 cut(s) 449
BspACI CCGC 5 cut(s) 574, 848, 1026, 1031, 1098
BspANI GGCC 3 cut(s) 10, 282, 1190
BspCNI CTCAG 7 cut(s) 13, 64, 220, 429, 725, 757, 1141
BspLI GGNNCC 5 cut(s) 176, 672, 822, 1035, 1071
BspMI ACCTGC 3 cut(s) 489, 1094, 1238
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 4 cut(s) 169, 182, 597, 1036
BspT104I TTCGAA 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 841
BsrFI RCCGGY 1 cut(s) 880
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 2 cut(s) 214, 684
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 3 cut(s) 136, 742, 948
BssMI GATC 4 cut(s) 130, 174, 589, 1041
BssSI CACGAG 2 cut(s) 656, 1260
Bst2BI CACGAG 2 cut(s) 656, 1260
Bst2UI CCWGG 4 cut(s) 137, 744, 949, 1074
Bst4CI ACNGT 4 cut(s) 148, 514, 690, 812
Bst6I CTCTTC 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 664
BstBI TTCGAA 1 cut(s) 611
BstC8I GCNNGC 5 cut(s) 304, 308, 486, 726, 846
BstDEI CTNAG 8 cut(s) 21, 51, 207, 416, 449, 712, 765, 1149
BstEII GGTNACC 1 cut(s) 410
BstF5I GGATG 3 cut(s) 992, 1121, 1180
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 4 cut(s) 133, 177, 592, 1044
BstMBI GATC 4 cut(s) 130, 174, 589, 1041
BstMWI GCNNNNNNNGC 6 cut(s) 356, 365, 896, 1145, 1154, 1253
BstNI CCWGG 4 cut(s) 137, 744, 949, 1074
BstNSI RCATGY 2 cut(s) 848, 925
BstPI GGTNACC 1 cut(s) 410
BstSCI CCNGG 5 cut(s) 135, 443, 742, 947, 1072
BstV1I GCAGC 6 cut(s) 390, 448, 791, 1144, 1166, 1243
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 3 cut(s) 10, 282, 1190
BtsCI GGATG 3 cut(s) 992, 1121, 1180
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 4 cut(s) 51, 424, 817, 1037
BveI ACCTGC 3 cut(s) 489, 1094, 1238
Cac8I GCNNGC 5 cut(s) 304, 308, 486, 726, 846
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 3 cut(s) 211, 652, 1189
CseI GACGC 1 cut(s) 1262
Csp6I GTAC 2 cut(s) 342, 665
CspAI ACCGGT 1 cut(s) 880
CviAII CATG 4 cut(s) 634, 845, 869, 922
CviQI GTAC 2 cut(s) 342, 665
DdeI CTNAG 8 cut(s) 21, 51, 207, 416, 449, 712, 765, 1149
DpnI GATC 4 cut(s) 132, 176, 591, 1043
DpnII GATC 4 cut(s) 130, 174, 589, 1041
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 122
Eco147I AGGCCT 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 211, 652
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 2 cut(s) 939, 1085
Eco88I CYCGRG 1 cut(s) 1196
Eco91I GGTNACC 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 410
EcoRII CCWGG 4 cut(s) 135, 742, 947, 1072
EcoT38I GRGCYC 1 cut(s) 124
FaeI CATG 4 cut(s) 637, 848, 872, 925
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FatI CATG 4 cut(s) 633, 844, 868, 921
FauNDI CATATG 1 cut(s) 1165
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 6 cut(s) 379, 462, 780, 1155, 1158, 1257
FokI GGATG 3 cut(s) 979, 1128, 1187
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 6 cut(s) 379, 462, 780, 1155, 1158, 1257
FspBI CTAG 6 cut(s) 284, 434, 485, 491, 1089, 1124
GlaI GCGC 1 cut(s) 221
GluI GCNGC 6 cut(s) 379, 462, 780, 1155, 1158, 1257
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 3 cut(s) 10, 282, 1190
HapII CCGG 3 cut(s) 444, 881, 996
HgaI GACGC 1 cut(s) 1262
HhaI GCGC 1 cut(s) 222
Hin1II CATG 4 cut(s) 637, 848, 872, 925
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 1 cut(s) 732
HindII GTYRAC 1 cut(s) 732
HinfI GANTC 5 cut(s) 17, 388, 961, 1057, 1092
HpaII CCGG 3 cut(s) 444, 881, 996
HphI GGTGA 3 cut(s) 125, 212, 1262
Hpy166II GTNNAC 1 cut(s) 732
Hpy188I TCNGA 7 cut(s) 22, 54, 254, 766, 916, 1065, 1274
Hpy188III TCNNGA 5 cut(s) 491, 674, 694, 773, 965
Hpy8I GTNNAC 1 cut(s) 732
HpyAV CCTTC 5 cut(s) 188, 230, 316, 1060, 1165
HpyCH4III ACNGT 4 cut(s) 148, 514, 690, 812
HpyCH4IV ACGT 1 cut(s) 827
HpyCH4V TGCA 8 cut(s) 227, 302, 500, 642, 800, 834, 1103, 1139
HpyF10VI GCNNNNNNNGC 6 cut(s) 356, 365, 896, 1145, 1154, 1253
HpyF3I CTNAG 8 cut(s) 21, 51, 207, 416, 449, 712, 765, 1149
HpySE526I ACGT 1 cut(s) 827
Hsp92II CATG 4 cut(s) 637, 848, 872, 925
HspAI GCGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 4 cut(s) 130, 174, 589, 1041
LmnI GCTCC 8 cut(s) 100, 119, 356, 446, 676, 1033, 1075, 1085
Lsp1109I GCAGC 6 cut(s) 390, 448, 791, 1144, 1166, 1243
LweI GCATC 3 cut(s) 809, 1001, 1126
MaeI CTAG 6 cut(s) 284, 434, 485, 491, 1089, 1124
MaeII ACGT 1 cut(s) 827
MaeIII GTNAC 3 cut(s) 369, 410, 1250
MalI GATC 4 cut(s) 132, 176, 591, 1043
MboI GATC 4 cut(s) 130, 174, 589, 1041
MboII GAAGA 4 cut(s) 75, 980, 1052, 1259
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 8 cut(s) 28, 230, 506, 582, 613, 971, 984, 1015
MnlI CCTC 7 cut(s) 34, 347, 425, 714, 769, 1144, 1214
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 2 cut(s) 26, 165
MslI CAYNNNNRTG 2 cut(s) 138, 867
MspA1I CMGCKG 3 cut(s) 461, 1098, 1157
MspI CCGG 3 cut(s) 444, 881, 996
MspR9I CCNGG 5 cut(s) 137, 445, 744, 949, 1074
Mva1269I GAATGC 3 cut(s) 502, 707, 1028
MvaI CCWGG 4 cut(s) 137, 744, 949, 1074
MwoI GCNNNNNNNGC 6 cut(s) 356, 365, 896, 1145, 1154, 1253
NciI CCSGG 1 cut(s) 445
NdeI CATATG 1 cut(s) 1165
NdeII GATC 4 cut(s) 130, 174, 589, 1041
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 4 cut(s) 637, 848, 872, 925
NlaIV GGNNCC 5 cut(s) 176, 672, 822, 1035, 1071
NmuCI GTSAC 1 cut(s) 1250
NspI RCATGY 2 cut(s) 848, 925
NspV TTCGAA 1 cut(s) 611
OliI CACNNNNGTG 1 cut(s) 138
PaeI GCATGC 1 cut(s) 848
PaqCI CACCTGC 1 cut(s) 1238
PceI AGGCCT 1 cut(s) 282
PctI GAATGC 3 cut(s) 502, 707, 1028
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 5 cut(s) 17, 388, 961, 1057, 1092
PinAI ACCGGT 1 cut(s) 880
PkrI GCNGC 6 cut(s) 380, 463, 781, 1156, 1159, 1258
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 124
Psp6I CCWGG 4 cut(s) 135, 742, 947, 1072
PspEI GGTNACC 1 cut(s) 410
PspGI CCWGG 4 cut(s) 135, 742, 947, 1072
PspN4I GGNNCC 5 cut(s) 176, 672, 822, 1035, 1071
PspPI GGNCC 3 cut(s) 211, 652, 1189
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 174
PvuII CAGCTG 2 cut(s) 461, 1157
RsaI GTAC 2 cut(s) 343, 666
RsaNI GTAC 2 cut(s) 342, 665
RseI CAYNNNNRTG 2 cut(s) 138, 867
SacI GAGCTC 1 cut(s) 124
SaqAI TTAA 2 cut(s) 26, 165
SatI GCNGC 6 cut(s) 379, 462, 780, 1155, 1158, 1257
Sau3AI GATC 4 cut(s) 130, 174, 589, 1041
Sau96I GGNCC 3 cut(s) 211, 652, 1189
ScrFI CCNGG 5 cut(s) 137, 445, 744, 949, 1074
SduI GDGCHC 1 cut(s) 124
SfaNI GCATC 3 cut(s) 809, 1001, 1126
SfuI TTCGAA 1 cut(s) 611
SinI GGWCC 2 cut(s) 211, 652
SmiMI CAYNNNNRTG 2 cut(s) 138, 867
SmlI CTYRAG 2 cut(s) 718, 1000
SmoI CTYRAG 2 cut(s) 718, 1000
SphI GCATGC 1 cut(s) 848
Sse9I AATT 8 cut(s) 28, 230, 506, 582, 613, 971, 984, 1015
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 5 cut(s) 574, 848, 1026, 1031, 1098
SspMI CTAG 6 cut(s) 284, 434, 485, 491, 1089, 1124
SstI GAGCTC 1 cut(s) 124
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 5 cut(s) 135, 443, 742, 947, 1072
TaaI ACNGT 4 cut(s) 148, 514, 690, 812
TaiI ACGT 1 cut(s) 830
TaqI TCGA 4 cut(s) 82, 320, 534, 611
TasI AATT 8 cut(s) 28, 230, 506, 582, 613, 971, 984, 1015
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 5 cut(s) 17, 388, 961, 1057, 1092
Tru1I TTAA 2 cut(s) 26, 165
Tru9I TTAA 2 cut(s) 26, 165
TscAI CASTG 4 cut(s) 51, 424, 817, 1044
TseFI GTSAC 1 cut(s) 1250
TseI GCWGC 6 cut(s) 378, 461, 779, 1154, 1157, 1256
Tsp45I GTSAC 1 cut(s) 1250
TspDTI ATGAA 5 cut(s) 276, 870, 1152, 1191, 1197
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 4 cut(s) 51, 424, 817, 1044
VpaK11BI GGWCC 2 cut(s) 211, 652
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 3 cut(s) 28, 582, 971
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 2 cut(s) 848, 925
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 6 cut(s) 284, 434, 485, 491, 1089, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.