FvH4_4g06430

Galacturonokinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003653
Physical Location & Seq
Reverse (-)
5724178 .. 5735009
10832 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06430.t8

Sequence Viewer

Length: 1266 bp
ATGGGTGGATCCTCTTGGCCTTCTGAAACTCAGTTAAATGAAATTAGAGAGATAGTTTCAGAAATGTCTGGGAGAGGTAGGGAACAAGTCCGGGTCGTGGCTTCTCCTTATCGAATTTGTCCACTCGGAGCTCATATTGATCACCAGGGCGGCATAGTATCTGCTATGACGATTAACAGGGGAATACTTCTAGGTTTTGTTCCTTCTGGTGACAACCAGGTTATTCTACGGTCAGGACAGTTCAAAGGCAGAGTCAGGTTCAGAATTGATGAAGTTTCGTGCCCTATGAACAATGGAAATGATGCTTCCAAAAGGGACGAAGAAAGTGATTGGGGAAGTTATGCAAGAGGAGCTGTATATGCATTACAGAGTAAGAAGACCTGTCTTGTCCAGGGTATCATTGGATACATATGTGGTACTGAAGGTATGGACAGTTCAGGCGTTAGCTCTTCAGCTGCTGTTGGTGTAGCTTACCTAATGGCTTTGGAAAATGCAAACAATTTGATTGTGTCCCCTGAAGAAAATATTGAGTTTGATCGGCTGATTGAAAATGAATTTCGGGGCCTGAGGAATGGTATATTGGACCAATCAGCCATATTACTGTCAAGCTATGGTTCTCTACTATGCATGAACTGCAAGACTAAAGAGCATAAGCTTGTACATCCGCCGAAATTGGGAAAGAACCACGAGACGGAGTGGCAGGAAGCGTACAAAATATTGTTGGCATTTTCAGGCTTAAAGCAAGCTTTAACTGAAAATTCTGGATATAATCGTCGAGTTGGAGAGTGTCAAGAGGCAGCTACGGTTCTTTTGAATGCGTCTGGAAATGGTGCAGCAGAGCCAGTCCTATCTAATGTTGAGCCAGAAGCTTATCAAACTCACAAGCACGTATTGAAACCTAATCTAGCCAAAAGAGCCGAGCATTTTTTCTCTGAGAATATGCGGGTTATAAAAGGACTTGAAGCTTGGGCTTCAGGGAGGTTCAAAGATTTTGGCACACTCATTACCGAATCAGGCTTGAGTTCGATTCAGAACTATGAATGTGGTTCTAAACCTCTGATACAATTGCGTGAGATCGTCCTAAGGGCTCCTGGTGTCTTTGGAGCACGGTTCAGTGGTGCCGGATTTAGAGGGTGCTGTGTTGCACTGGTAGATGCCAATCTTGCAGCTGAAGCTGCATCGTTTGTCAGGGAAGAGTATAGCAAGCTCCAGCCTGACTTGGATGTCAACAAAAAGACATACAAGGGAGCAACCTTACCAGATTGA

Protein Analysis

422

Amino Acids

45.9

Weight (kDa)

6.49

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 49 - 106 4e-08 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 950
AasI GACNNNNNNGTC 1 cut(s) 1223
AccB1I GGYRCC 1 cut(s) 1118
AciI CCGC 3 cut(s) 150, 665, 943
AclWI GGATC 2 cut(s) 3, 16
AcsI RAATTY 3 cut(s) 114, 554, 757
AcuI CTGAAG 5 cut(s) 435, 441, 537, 957, 1191
AfaI GTAC 3 cut(s) 418, 660, 710
AfiI CCNNNNNNNGG 3 cut(s) 97, 674, 691
AgsI TTSAA 6 cut(s) 244, 548, 814, 895, 962, 985
AjnI CCWGG 4 cut(s) 144, 216, 390, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
Alw21I GWGCWC 2 cut(s) 133, 1108
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 3, 16
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 2 cut(s) 17, 562
ApeKI GCWGC 5 cut(s) 455, 797, 833, 1166, 1175
ApoI RAATTY 3 cut(s) 114, 554, 757
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspS9I GGNCC 2 cut(s) 562, 583
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 134, 221
AvaII GGWCC 1 cut(s) 583
AxyI CCTNAGG 2 cut(s) 566, 1082
BaeGI GKGCMC 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 1118
BanII GRGCYC 2 cut(s) 133, 1090
BauI CACGAG 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 2 cut(s) 133, 1108
BbvI GCAGC 5 cut(s) 442, 809, 845, 1162, 1178
BciT130I CCWGG 4 cut(s) 146, 218, 392, 1092
BciVI GTATCC 1 cut(s) 398
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 2 cut(s) 191, 905
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 6 cut(s) 151, 456, 798, 834, 1167, 1176
BlsI GCNGC 6 cut(s) 152, 457, 799, 835, 1168, 1177
Bme1390I CCNGG 5 cut(s) 92, 146, 218, 392, 1092
Bme18I GGWCC 1 cut(s) 583
BmgT120I GGNCC 2 cut(s) 562, 583
BmiI GGNNCC 4 cut(s) 10, 563, 1089, 1120
BmrFI CCNGG 5 cut(s) 92, 146, 218, 392, 1092
BmsI GCATC 3 cut(s) 292, 1144, 1187
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 1193
BpuEI CTTGAG 1 cut(s) 1039
BpuMI CCSGG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 1158
BsaJI CCNNGG 2 cut(s) 145, 391
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 674, 691
Bse1I ACTGG 2 cut(s) 842, 1152
Bse21I CCTNAGG 2 cut(s) 566, 1082
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 4 cut(s) 146, 218, 392, 1092
BseDI CCNNGG 2 cut(s) 145, 391
BseGI GGATG 2 cut(s) 661, 1228
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 97, 674, 691
BseMII CTCAG 3 cut(s) 44, 557, 924
BseNI ACTGG 2 cut(s) 842, 1152
BseRI GAGGAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 5 cut(s) 442, 809, 845, 1162, 1178
BsgI GTGCAG 1 cut(s) 852
BshFI GGCC 2 cut(s) 19, 564
BshNI GGYRCC 1 cut(s) 1118
BsiHKAI GWGCWC 2 cut(s) 133, 1108
BsiSI CCGG 2 cut(s) 91, 1122
BslFI GGGAC 2 cut(s) 329, 496
BslI CCNNNNNNNGG 3 cut(s) 97, 674, 691
BsmAI GTCTC 1 cut(s) 683
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 2 cut(s) 329, 496
BsmI GAATGC 1 cut(s) 820
BsnI GGCC 2 cut(s) 19, 564
Bsp1286I GDGCHC 4 cut(s) 133, 284, 1090, 1108
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 4 cut(s) 8, 139, 535, 1074
BspACI CCGC 3 cut(s) 150, 665, 943
BspANI GGCC 2 cut(s) 19, 564
BspCNI CTCAG 3 cut(s) 43, 558, 925
BspLI GGNNCC 4 cut(s) 10, 563, 1089, 1120
BspPI GGATC 2 cut(s) 3, 16
BspQI GCTCTTC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 1118
BsrGI TGTACA 1 cut(s) 658
BsrI ACTGG 2 cut(s) 842, 1152
BssECI CCNNGG 2 cut(s) 145, 391
BssMI GATC 4 cut(s) 8, 139, 535, 1074
BssSI CACGAG 1 cut(s) 686
Bst2BI CACGAG 1 cut(s) 686
Bst2UI CCWGG 4 cut(s) 146, 218, 392, 1092
Bst4CI ACNGT 6 cut(s) 231, 240, 434, 603, 805, 1110
Bst6I CTCTTC 2 cut(s) 454, 1188
BstAPI GCANNNNNTGC 1 cut(s) 633
BstAUI TGTACA 1 cut(s) 658
BstBAI YACGTR 1 cut(s) 889
BstC8I GCNNGC 2 cut(s) 744, 1205
BstDEI CTNAG 4 cut(s) 30, 566, 933, 1082
BstF5I GGATG 2 cut(s) 661, 1228
BstKTI GATC 4 cut(s) 11, 142, 538, 1077
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 4 cut(s) 8, 139, 535, 1074
BstMWI GCNNNNNNNGC 7 cut(s) 350, 359, 633, 914, 1163, 1172, 1175
BstNI CCWGG 4 cut(s) 146, 218, 392, 1092
BstSCI CCNGG 5 cut(s) 90, 144, 216, 390, 1090
BstSLI GKGCMC 1 cut(s) 284
BstV1I GCAGC 5 cut(s) 442, 809, 845, 1162, 1178
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
Bsu36I CCTNAGG 2 cut(s) 566, 1082
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 2 cut(s) 19, 564
BtsCI GGATG 2 cut(s) 661, 1228
BtsIMutI CAGTG 2 cut(s) 1120, 1145
Cac8I GCNNGC 2 cut(s) 744, 1205
CaiI CAGNNNCTG 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 562, 583
CseI GACGC 1 cut(s) 807
CsiI ACCWGGT 1 cut(s) 216
Csp6I GTAC 3 cut(s) 417, 659, 709
CviAII CATG 1 cut(s) 628
CviQI GTAC 3 cut(s) 417, 659, 709
DdeI CTNAG 4 cut(s) 30, 566, 933, 1082
DpnI GATC 4 cut(s) 10, 141, 537, 1076
DpnII GATC 4 cut(s) 8, 139, 535, 1074
DrdI GACNNNNNNGTC 1 cut(s) 1223
DseDI GACNNNNNNGTC 1 cut(s) 1223
Eam1104I CTCTTC 2 cut(s) 454, 1188
EarI CTCTTC 2 cut(s) 454, 1188
EciI GGCGGA 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 133, 1090
Eco47I GGWCC 1 cut(s) 583
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 5 cut(s) 435, 441, 537, 957, 1191
Eco81I CCTNAGG 2 cut(s) 566, 1082
EcoICRI GAGCTC 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 4 cut(s) 144, 216, 390, 1090
EcoT22I ATGCAT 2 cut(s) 364, 629
EcoT38I GRGCYC 2 cut(s) 133, 1090
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 1 cut(s) 631
FaqI GGGAC 2 cut(s) 329, 496
FatI CATG 1 cut(s) 627
FauI CCCGC 1 cut(s) 936
FauNDI CATATG 1 cut(s) 410
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 6 cut(s) 151, 456, 798, 834, 1167, 1176
FokI GGATG 2 cut(s) 648, 1235
FriOI GRGCYC 2 cut(s) 133, 1090
Fsp4HI GCNGC 6 cut(s) 151, 456, 798, 834, 1167, 1176
FspBI CTAG 2 cut(s) 191, 905
GluI GCNGC 6 cut(s) 151, 456, 798, 834, 1167, 1176
GsuI CTGGAG 1 cut(s) 1193
HaeIII GGCC 2 cut(s) 19, 564
HapII CCGG 2 cut(s) 91, 1122
HgaI GACGC 1 cut(s) 807
Hin1II CATG 1 cut(s) 631
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HindIII AAGCTT 4 cut(s) 653, 744, 867, 963
HinfI GANTC 3 cut(s) 252, 1010, 1027
HpaII CCGG 2 cut(s) 91, 1122
HphI GGTGA 2 cut(s) 134, 221
Hpy166II GTNNAC 2 cut(s) 122, 1228
Hpy188I TCNGA 7 cut(s) 25, 61, 128, 263, 934, 1032, 1059
Hpy188III TCNNGA 4 cut(s) 234, 762, 791, 822
Hpy8I GTNNAC 2 cut(s) 122, 1228
Hpy99I CGWCG 1 cut(s) 777
HpyAV CCTTC 3 cut(s) 30, 213, 416
HpyCH4III ACNGT 6 cut(s) 231, 240, 434, 603, 805, 1110
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 9 cut(s) 344, 362, 494, 627, 636, 833, 1145, 1166, 1178
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 359, 633, 914, 1163, 1172, 1175
HpyF3I CTNAG 4 cut(s) 30, 566, 933, 1082
HpySE526I ACGT 1 cut(s) 888
Hsp92II CATG 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 4 cut(s) 8, 139, 535, 1074
LguI GCTCTTC 1 cut(s) 454
LmnI GCTCC 6 cut(s) 128, 350, 1093, 1103, 1212, 1247
Lsp1109I GCAGC 5 cut(s) 442, 809, 845, 1162, 1178
LweI GCATC 3 cut(s) 292, 1144, 1187
MabI ACCWGGT 1 cut(s) 216
MaeI CTAG 2 cut(s) 191, 905
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 1 cut(s) 209
MalI GATC 4 cut(s) 10, 141, 537, 1076
MboI GATC 4 cut(s) 8, 139, 535, 1074
MboII GAAGA 5 cut(s) 332, 388, 441, 530, 1205
MfeI CAATTG 1 cut(s) 1064
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 4 cut(s) 133, 284, 1090, 1108
MluCI AATT 8 cut(s) 42, 114, 264, 499, 554, 671, 757, 1064
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 760
MnlI CCTC 8 cut(s) 22, 68, 341, 561, 787, 972, 1065, 1124
Mph1103I ATGCAT 2 cut(s) 364, 629
MseI TTAA 4 cut(s) 35, 174, 737, 749
MspA1I CMGCKG 2 cut(s) 455, 1169
MspI CCGG 2 cut(s) 91, 1122
MspR9I CCNGG 5 cut(s) 92, 146, 218, 392, 1092
MunI CAATTG 1 cut(s) 1064
Mva1269I GAATGC 1 cut(s) 820
MvaI CCWGG 4 cut(s) 146, 218, 392, 1092
MwoI GCNNNNNNNGC 7 cut(s) 350, 359, 633, 914, 1163, 1172, 1175
NciI CCSGG 1 cut(s) 92
NdeI CATATG 1 cut(s) 410
NdeII GATC 4 cut(s) 8, 139, 535, 1074
NlaIII CATG 1 cut(s) 631
NlaIV GGNNCC 4 cut(s) 10, 563, 1089, 1120
NmeAIII GCCGAG 1 cut(s) 943
NmuCI GTSAC 1 cut(s) 209
NsiI ATGCAT 2 cut(s) 364, 629
PciSI GCTCTTC 1 cut(s) 454
PctI GAATGC 1 cut(s) 820
PfeI GAWTC 2 cut(s) 1010, 1027
PkrI GCNGC 6 cut(s) 152, 457, 799, 835, 1168, 1177
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 889
PsiI TTATAA 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 4 cut(s) 144, 216, 390, 1090
PspGI CCWGG 4 cut(s) 144, 216, 390, 1090
PspN4I GGNNCC 4 cut(s) 10, 563, 1089, 1120
PspPI GGNCC 2 cut(s) 562, 583
PsrI GAACNNNNNNTAC 2 cut(s) 418, 450
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 2 cut(s) 455, 1169
RsaI GTAC 3 cut(s) 418, 660, 710
RsaNI GTAC 3 cut(s) 417, 659, 709
SacI GAGCTC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 454
SaqAI TTAA 4 cut(s) 35, 174, 737, 749
SatI GCNGC 6 cut(s) 151, 456, 798, 834, 1167, 1176
Sau3AI GATC 4 cut(s) 8, 139, 535, 1074
Sau96I GGNCC 2 cut(s) 562, 583
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 5 cut(s) 92, 146, 218, 392, 1092
SduI GDGCHC 4 cut(s) 133, 284, 1090, 1108
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 3 cut(s) 292, 1144, 1187
SinI GGWCC 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
Sse9I AATT 8 cut(s) 42, 114, 264, 499, 554, 671, 757, 1064
SsiI CCGC 3 cut(s) 150, 665, 943
SspI AATATT 2 cut(s) 526, 717
SspMI CTAG 2 cut(s) 191, 905
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 5 cut(s) 90, 144, 216, 390, 1090
TaaI ACNGT 6 cut(s) 231, 240, 434, 603, 805, 1110
TaiI ACGT 1 cut(s) 891
TaqI TCGA 3 cut(s) 112, 775, 1025
TasI AATT 8 cut(s) 42, 114, 264, 499, 554, 671, 757, 1064
TatI WGTACW 1 cut(s) 658
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 1010, 1027
Tru1I TTAA 4 cut(s) 35, 174, 737, 749
Tru9I TTAA 4 cut(s) 35, 174, 737, 749
TscAI CASTG 2 cut(s) 1120, 1152
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 5 cut(s) 455, 797, 833, 1166, 1175
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 6 cut(s) 54, 285, 302, 567, 644, 1053
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 2 cut(s) 1120, 1152
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 3 cut(s) 114, 554, 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 2 cut(s) 191, 905
Zsp2I ATGCAT 2 cut(s) 364, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.