Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009285

Physical Location & Seq

Genomic Coordinates Reverse (-)

3972180 .. 3974440

Gene Structure 2261 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G12530.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 633 bp

ATGGCTGGCCAGACTGATCCTCATATTTCACTGTTTTCTCCACAAGAGGTTGAGTTTATGGCAGAGGACGAGCTGGTCGAGATTGTTCCTAATATGAACATGGAGCAACTCAATTTCATCAGTGGCGATTTCGGTCGGTTCATTCCTCAGATTCCGACAAAGGTGCCTCTGTGGCTTGCAGTGGCACTGAAGAGGAGAGGAAAATGCACTTTCCGGCCTCCGGGGTGGATGTCTGTTGATAATTTGACTCAGATCTTGGAAGCAGAACGTGAATCTCAGTCGACATTCCAGGCATTACCATTTAGTTACGTGGAGATTGCCAGACTACTTTTTGACCATGCACGTGACGATATTCCAGACATGTATATGGTGAGATCCCTAGTCGAGGACATCAGAGATGTACGGCTTCACAAACTTGAGACTAACTTAGGATCATTTCAAGGTACATCTGCAGTAAAGATCTCTAATGTATCGGCAATGGAAGTAAACATAGTACGCCCATTTGTGATAAGAGCCTTAGAAGCATTTTATAAGCATGACAAGCCAGAGGCTGATGTAGACAGAGACACCAGAAGTAGTAGACAACAGCGTGAAACCAACAATGAACCAAGGAGACCTCTAAGGCAACGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0000811 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0022402 GO:0031261 GO:0031298 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044786 GO:0046483 GO:0050896 GO:0051716 GO:0070013 GO:0071704 GO:0090304 GO:1901360 GO:1901576 GO:1902292 GO:1902315 GO:1902969 GO:1902975 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sld5

Protein Analysis

210

Amino Acids

24.29

Weight (kDa)

5.66

Isoelectric Point (pI)

59.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PSF2_N	PF25005	13 - 70	7.3e-28	PSF2 N-terminal domain
Sld5	PF05916	76 - 171	4.2e-15	GINS complex protein helical bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12530 AT3G12530 AT3G12530 AT3G12530
fragaria_vesca	FvH4_7g22520
malus_domestica	MD01G1134500.v1.1 MD07G1199100.v1.1
prunus_persica	Prupe.2G235900_v2.0.a1 Prupe.2G235900_v2.0.a1
pyrus_communis	pycom01g15520 pycom07g18620
rosa_chinensis	RchiOBHm_Chr1g0367201
rosa_laevigata	RLG00000027292
rosa_multiflora	Rmu_sc0006367.1_g000002
rosa_roxburghii	Rroxscaffold_4G00289550
rosa_rugosa	Rorug01G0335100 Rorug01G0335200
rosa_samantha	Rh1AG342000 Rh1BG302400 Rh1CG318800 Rh1DG334900
rosa_wichuraiana	Rw1G030390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	531
AasI	GACNNNNNNGTC	1 cut(s)	74
AccB1I	GGYRCC	1 cut(s)	163
AccI	GTMKAC	3 cut(s)	281, 558, 580
AclWI	GGATC	3 cut(s)	11, 369, 439
AcoI	YGGCCR	1 cut(s)	7
AcuI	CTGAAG	1 cut(s)	209
AcvI	CACGTG	1 cut(s)	344
AfaI	GTAC	3 cut(s)	402, 445, 495
AfiI	CCNNNNNNNGG	2 cut(s)	220, 385
AflIII	ACRYGT	1 cut(s)	360
AgsI	TTSAA	1 cut(s)	440
AjnI	CCWGG	1 cut(s)	288
AluBI	AGCT	1 cut(s)	73
AluI	AGCT	1 cut(s)	73
Alw26I	GTCTC	3 cut(s)	413, 558, 607
AlwI	GGATC	3 cut(s)	11, 369, 439
AlwNI	CAGNNNCTG	1 cut(s)	551
AoxI	GGCC	2 cut(s)	7, 215
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	1 cut(s)	382
BalI	TGGCCA	1 cut(s)	9
BanI	GGYRCC	1 cut(s)	163
BbrPI	CACGTG	1 cut(s)	344
BceAI	ACGGC	1 cut(s)	419
BcgI	CGANNNNNNTGC	2 cut(s)	145, 179
BciT130I	CCWGG	1 cut(s)	290
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	3 cut(s)	413, 558, 607
BfaI	CTAG	2 cut(s)	380, 631
BfmI	CTRYAG	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	172
BglII	AGATCT	2 cut(s)	252, 459
Bme1390I	CCNGG	2 cut(s)	222, 290
BmiI	GGNNCC	1 cut(s)	165
BmrFI	CCNGG	2 cut(s)	222, 290
BpuEI	CTTGAG	1 cut(s)	437
BpuMI	CCSGG	1 cut(s)	222
BsaAI	YACGTR	2 cut(s)	310, 344
BsaI	GGTCTC	1 cut(s)	607
BsaJI	CCNNGG	2 cut(s)	221, 608
Bsc4I	CCNNNNNNNGG	2 cut(s)	220, 385
Bse3DI	GCAATG	1 cut(s)	483
BseBI	CCWGG	1 cut(s)	290
BseDI	CCNNGG	2 cut(s)	221, 608
BseGI	GGATG	1 cut(s)	234
BseLI	CCNNNNNNNGG	2 cut(s)	220, 385
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	3 cut(s)	161, 263, 290
BseRI	GAGGAG	1 cut(s)	208
Bsh1285I	CGRYCG	1 cut(s)	136
BshFI	GGCC	2 cut(s)	9, 217
BshNI	GGYRCC	1 cut(s)	163
BsiEI	CGRYCG	1 cut(s)	136
BsiSI	CCGG	2 cut(s)	214, 221
BslI	CCNNNNNNNGG	2 cut(s)	220, 385
BsmAI	GTCTC	3 cut(s)	413, 558, 607
BsnI	GGCC	2 cut(s)	9, 217
Bso31I	GGTCTC	1 cut(s)	607
Bsp143I	GATC	5 cut(s)	16, 252, 374, 431, 459
BspANI	GGCC	2 cut(s)	9, 217
BspCNI	CTCAG	3 cut(s)	160, 262, 289
BspLI	GGNNCC	1 cut(s)	165
BspMAI	CTGCAG	1 cut(s)	454
BspPI	GGATC	3 cut(s)	11, 369, 439
BspT107I	GGYRCC	1 cut(s)	163
BspTNI	GGTCTC	1 cut(s)	607
BsrDI	GCAATG	1 cut(s)	483
BssECI	CCNNGG	2 cut(s)	221, 608
BssMI	GATC	5 cut(s)	16, 252, 374, 431, 459
BssT1I	CCWWGG	1 cut(s)	608
Bst2UI	CCWGG	1 cut(s)	290
Bst4CI	ACNGT	1 cut(s)	33
Bst6I	CTCTTC	1 cut(s)	185
BstBAI	YACGTR	2 cut(s)	310, 344
BstC8I	GCNNGC	2 cut(s)	7, 177
BstDEI	CTNAG	6 cut(s)	147, 249, 276, 427, 517, 620
BstENI	CCTNNNNNAGG	1 cut(s)	383
BstF5I	GGATG	1 cut(s)	234
BstKTI	GATC	5 cut(s)	19, 255, 377, 434, 462
BstMAI	GTCTC	3 cut(s)	413, 558, 607
BstMBI	GATC	5 cut(s)	16, 252, 374, 431, 459
BstMCI	CGRYCG	1 cut(s)	136
BstMWI	GCNNNNNNNGC	3 cut(s)	172, 521, 541
BstNI	CCWGG	1 cut(s)	290
BstNSI	RCATGY	1 cut(s)	364
BstSCI	CCNGG	2 cut(s)	220, 288
BstSFI	CTRYAG	1 cut(s)	450
BstX2I	RGATCY	3 cut(s)	252, 374, 459
BstYI	RGATCY	3 cut(s)	252, 374, 459
BsuRI	GGCC	2 cut(s)	9, 217
BtsCI	GGATG	1 cut(s)	234
BtsI	GCAGTG	1 cut(s)	186
BtsIMutI	CAGTG	4 cut(s)	29, 127, 185, 186
Cac8I	GCNNGC	2 cut(s)	7, 177
CaiI	CAGNNNCTG	1 cut(s)	551
Csp6I	GTAC	3 cut(s)	401, 444, 494
CviAII	CATG	4 cut(s)	100, 338, 361, 536
CviJI	RGCY	9 cut(s)	5, 9, 73, 175, 217, 406, 515, 544, 551
CviKI_1	RGCY	9 cut(s)	5, 9, 73, 175, 217, 406, 515, 544, 551
CviQI	GTAC	3 cut(s)	401, 444, 494
DdeI	CTNAG	6 cut(s)	147, 249, 276, 427, 517, 620
DpnI	GATC	5 cut(s)	18, 254, 376, 433, 461
DpnII	GATC	5 cut(s)	16, 252, 374, 431, 459
DrdI	GACNNNNNNGTC	1 cut(s)	74
DseDI	GACNNNNNNGTC	1 cut(s)	74
EaeI	YGGCCR	1 cut(s)	7
Eam1104I	CTCTTC	1 cut(s)	185
EarI	CTCTTC	1 cut(s)	185
Eco130I	CCWWGG	1 cut(s)	608
Eco31I	GGTCTC	1 cut(s)	607
Eco57I	CTGAAG	1 cut(s)	209
Eco72I	CACGTG	1 cut(s)	344
EcoNI	CCTNNNNNAGG	1 cut(s)	383
EcoRII	CCWGG	1 cut(s)	288
EcoT14I	CCWWGG	1 cut(s)	608
ErhI	CCWWGG	1 cut(s)	608
FaeI	CATG	4 cut(s)	103, 341, 364, 539
FatI	CATG	4 cut(s)	99, 337, 360, 535
FblI	GTMKAC	3 cut(s)	281, 558, 580
FokI	GGATG	1 cut(s)	241
FspBI	CTAG	2 cut(s)	380, 631
HaeIII	GGCC	2 cut(s)	9, 217
HapII	CCGG	2 cut(s)	214, 221
Hin1II	CATG	4 cut(s)	103, 341, 364, 539
HincII	GTYRAC	1 cut(s)	282
HindII	GTYRAC	1 cut(s)	282
HinfI	GANTC	3 cut(s)	151, 247, 272
HpaII	CCGG	2 cut(s)	214, 221
HphI	GGTGA	1 cut(s)	382
Hpy166II	GTNNAC	4 cut(s)	282, 487, 559, 581
Hpy188I	TCNGA	4 cut(s)	150, 156, 252, 395
Hpy188III	TCNNGA	2 cut(s)	79, 356
Hpy8I	GTNNAC	4 cut(s)	282, 487, 559, 581
HpyCH4III	ACNGT	1 cut(s)	33
HpyCH4IV	ACGT	3 cut(s)	268, 309, 343
HpyCH4V	TGCA	4 cut(s)	179, 207, 341, 452
HpyF10VI	GCNNNNNNNGC	3 cut(s)	172, 521, 541
HpyF3I	CTNAG	6 cut(s)	147, 249, 276, 427, 517, 620
HpySE526I	ACGT	3 cut(s)	268, 309, 343
Hsp92II	CATG	4 cut(s)	103, 341, 364, 539
Kzo9I	GATC	5 cut(s)	16, 252, 374, 431, 459
LmnI	GCTCC	1 cut(s)	103
MaeI	CTAG	2 cut(s)	380, 631
MaeII	ACGT	3 cut(s)	268, 309, 343
MaeIII	GTNAC	2 cut(s)	305, 344
MalI	GATC	5 cut(s)	18, 254, 376, 433, 461
MboI	GATC	5 cut(s)	16, 252, 374, 431, 459
MboII	GAAGA	1 cut(s)	202
MflI	RGATCY	3 cut(s)	252, 374, 459
MlsI	TGGCCA	1 cut(s)	9
MluCI	AATT	2 cut(s)	112, 241
MluNI	TGGCCA	1 cut(s)	9
MlyI	GAGTC	1 cut(s)	241
MmeI	TCCRAC	1 cut(s)	179
Mox20I	TGGCCA	1 cut(s)	9
MscI	TGGCCA	1 cut(s)	9
MslI	CAYNNNNRTG	2 cut(s)	342, 365
Msp20I	TGGCCA	1 cut(s)	9
MspI	CCGG	2 cut(s)	214, 221
MspR9I	CCNGG	2 cut(s)	222, 290
MvaI	CCWGG	1 cut(s)	290
MwoI	GCNNNNNNNGC	3 cut(s)	172, 521, 541
NciI	CCSGG	1 cut(s)	222
NdeII	GATC	5 cut(s)	16, 252, 374, 431, 459
NlaIII	CATG	4 cut(s)	103, 341, 364, 539
NlaIV	GGNNCC	1 cut(s)	165
NmuCI	GTSAC	1 cut(s)	344
NspI	RCATGY	1 cut(s)	364
PciI	ACATGT	1 cut(s)	360
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PfeI	GAWTC	2 cut(s)	151, 272
PleI	GAGTC	1 cut(s)	241
PmaCI	CACGTG	1 cut(s)	344
PmlI	CACGTG	1 cut(s)	344
PpsI	GAGTC	1 cut(s)	241
Ppu21I	YACGTR	2 cut(s)	310, 344
PscI	ACATGT	1 cut(s)	360
PsiI	TTATAA	1 cut(s)	531
Psp6I	CCWGG	1 cut(s)	288
PspCI	CACGTG	1 cut(s)	344
PspGI	CCWGG	1 cut(s)	288
PspN4I	GGNNCC	1 cut(s)	165
PstI	CTGCAG	1 cut(s)	454
PstNI	CAGNNNCTG	1 cut(s)	551
PsuI	RGATCY	3 cut(s)	252, 374, 459
RsaI	GTAC	3 cut(s)	402, 445, 495
RsaNI	GTAC	3 cut(s)	401, 444, 494
RseI	CAYNNNNRTG	2 cut(s)	342, 365
SalI	GTCGAC	1 cut(s)	280
Sau3AI	GATC	5 cut(s)	16, 252, 374, 431, 459
SchI	GAGTC	1 cut(s)	241
ScrFI	CCNGG	2 cut(s)	222, 290
SetI	ASST	8 cut(s)	51, 75, 165, 271, 312, 346, 445, 619
SfcI	CTRYAG	1 cut(s)	450
SmiMI	CAYNNNNRTG	2 cut(s)	342, 365
SmlI	CTYRAG	1 cut(s)	416
SmoI	CTYRAG	1 cut(s)	416
Sse9I	AATT	2 cut(s)	112, 241
SspMI	CTAG	2 cut(s)	380, 631
StyD4I	CCNGG	2 cut(s)	220, 288
StyI	CCWWGG	1 cut(s)	608
TaaI	ACNGT	1 cut(s)	33
TaiI	ACGT	3 cut(s)	271, 312, 346
TaqI	TCGA	3 cut(s)	78, 281, 384
TaqII	GACCGA	1 cut(s)	122
TasI	AATT	2 cut(s)	112, 241
TfiI	GAWTC	2 cut(s)	151, 272
TscAI	CASTG	4 cut(s)	36, 127, 186, 192
TseFI	GTSAC	1 cut(s)	344
Tsp45I	GTSAC	1 cut(s)	344
TspDTI	ATGAA	4 cut(s)	106, 110, 130, 618
TspRI	CASTG	4 cut(s)	36, 127, 186, 192
XagI	CCTNNNNNAGG	1 cut(s)	383
XceI	RCATGY	1 cut(s)	364
XmiI	GTMKAC	3 cut(s)	281, 558, 580
XspI	CTAG	2 cut(s)	380, 631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.