AT3G14240

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0017066
Physical Location & Seq
Reverse (-)
4741287 .. 4744263
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14240.1

Sequence Viewer

Length: 2328 bp
ATGGCTTTCTTCTTCTACTTTTTCTTCCTCTTGACTCTTTCTTCTCCTTCTTCTTCTGCTTCCTCTTCAAACTCCTTGACTTACATCGTCCACGTCGACCACGAAGCTAAACCCTCAATCTTCCCCACTCATTTTCACTGGTACACTTCCTCTCTTGCCTCACTCACCTCTTCTCCTCCCTCCATTATCCACACATACGACACCGTTTTCCATGGCTTCTCCGCTAGACTCACTTCACAAGACGCTAGTCAACTCTTAGACCATCCTCATGTCATCTCTGTTATCCCGGAACAAGTCCGTCACTTGCACACCACTCGTTCCCCTGAGTTTCTTGGTCTTAGGTCCACCGACAAAGCGGGTCTACTCGAAGAGTCTGATTTCGGGTCGGATCTAGTTATCGGAGTTATCGATACTGGTGTTTGGCCCGAAAGACCTAGCTTTGATGACCGTGGTCTTGGTCCTGTTCCCATTAAATGGAAAGGCCAATGTATCGCTTCCCAAGATTTTCCGGAGTCTGCTTGTAACCGTAAACTCGTCGGAGCTAGATTCTTCTGCGGTGGGTATGAAGCAACCAACGGGAAAATGAATGAAACGACTGAGTTTCGCTCTCCGCGTGACTCCGATGGACATGGGACTCACACAGCTTCAATCTCCGCCGGCCGTTACGTTTTTCCGGCGTCAACTCTCGGCTACGCTCACGGCGTCGCTGCTGGGATGGCTCCAAAAGCTAGACTCGCCGCTTACAAAGTCTGTTGGAACTCCGGTTGTTACGACTCCGATATCTTAGCCGCTTTCGACACCGCCGTTGCCGACGGTGTCGATGTCATCTCTCTCTCCGTTGGAGGCGTCGTGGTTCCTTATTACCTAGACGCTATCGCTATTGGAGCTTTTGGAGCTATTGACAGAGGCATATTCGTCTCTGCTTCCGCCGGTAACGGAGGTCCCGGTGCTTTAACGGTGACAAATGTTGCTCCGTGGATGACAACAGTCGGAGCTGGAACAATCGATAGGGATTTTCCGGCCAATGTGAAACTCGGTAACGGGAAGATGATTTCTGGTGTTAGTGTATACGGTGGACCGGGTCTGGATCCGGGTCGAATGTACCCGCTTGTTTACGGTGGTAGTTTACTAGGCGGCGATGGTTACTCATCGTCTCTGTGTCTTGAAGGCTCGTTGGATCCGAATTTAGTGAAGGGAAAGATCGTTCTTTGTGATAGAGGAATCAATTCTAGAGCAACCAAAGGTGAGATCGTACGGAAAAATGGAGGCTTGGGGATGATTATAGCGAATGGTGTGTTTGACGGTGAAGGTTTAGTAGCTGATTGCCACGTGTTACCGGCGACATCTGTTGGTGCTTCTGGAGGAGATGAGATTCGTAGGTATATCTCTGAATCATCCAAATCTCGTTCATCGAAACATCCAACGGCTACGATTGTTTTCAAAGGTACCCGACTTGGGATCCGACCTGCACCGGTTGTTGCATCTTTCTCTGCTCGTGGTCCTAATCCAGAGACGCCGGAGATTCTTAAACCGGATGTAATCGCACCTGGTTTGAATATTCTAGCTGCTTGGCCTGACCGGATTGGTCCATCTGGTGTTACTTCTGATAACCGGAGAACTGAGTTCAACATTTTATCAGGCACTTCGATGGCGTGCCCGCACGTGTCTGGTCTAGCTGCTTTGCTTAAGGCGGCTCATCCGGATTGGAGTCCGGCGGCGATAAGATCAGCATTGATAACCACGGCTTACACGGTTGATAACAGCGGTGAGCCGATGATGGATGAGTCCACTGGCAATACATCTTCGGTTATGGATTATGGTTCGGGTCATGTTCACCCAACCAAAGCTATGGATCCGGGATTAGTCTATGATATAACATCTTATGATTATATCAACTTCTTGTGTAATTCTAACTACACTAGAACCAACATTGTGACAATAACCCGTCGCCAAGCGGACTGTGACGGTGCAAGACGAGCGGGACACGTCGGGAATTTAAACTACCCGTCGTTCTCTGTCGTGTTTCAGCAGTATGGAGAGAGTAAAATGTCGACACATTTCATTAGGACTGTGACAAATGTAGGCGATTCGGATTCGGTTTACGAGATTAAGATTAGGCCGCCTAGAGGGACTACGGTGACGGTTGAGCCGGAGAAGCTATCGTTCAGACGGGTGGGGCAGAAACTGAGTTTTGTTGTTAGGGTTAAGACCACAGAGGTGAAGTTGTCACCAGGAGCGACAAATGTGGAGACTGGTCATATAGTTTGGTCAGATGGGAAACGGAATGTGACAAGTCCCTTGGTGGTCACTCTTCAACAACCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

775

Amino Acids

82.58

Weight (kDa)

6.74

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 27 - 103 5.4e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 128 - 599 1.4e-49 Subtilase family
PA PF02225 368 - 458 6.4e-11 PA domain
fn3_6 PF17766 665 - 769 2.9e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1474
Acc65I GGTACC 1 cut(s) 1445
AccB1I GGYRCC 1 cut(s) 1445
AccB7I CCANNNNNTGG 1 cut(s) 474
AccBSI CCGCTC 1 cut(s) 1979
AccI GTMKAC 4 cut(s) 96, 361, 1068, 2051
AccII CGCG 1 cut(s) 613
AccIII TCCGGA 2 cut(s) 508, 1699
AclWI GGATC 9 cut(s) 396, 1082, 1095, 1172, 1185, 1453, 1466, 1847, 1860
AcoI YGGCCR 2 cut(s) 658, 1020
AcsI RAATTY 2 cut(s) 1183, 1993
AcvI CACGTG 2 cut(s) 1330, 1663
AcyI GRCGYC 4 cut(s) 677, 702, 846, 1514
AfaI GTAC 4 cut(s) 143, 1103, 1254, 1447
AfiI CCNNNNNNNGG 3 cut(s) 474, 1455, 2126
AflII CTTAAG 1 cut(s) 1685
AflIII ACRYGT 3 cut(s) 1329, 1662, 1984
AgeI ACCGGT 1 cut(s) 1471
AgsI TTSAA 7 cut(s) 69, 648, 1166, 1441, 1555, 1627, 2315
AjiI CACGTC 2 cut(s) 94, 1987
AjnI CCWGG 2 cut(s) 1546, 2230
AleI CACNNNNGTG 1 cut(s) 2216
AloI GAACNNNNNNTCC 2 cut(s) 1188, 1220
Alw26I GTCTC 4 cut(s) 922, 1158, 1505, 2243
AlwI GGATC 9 cut(s) 396, 1082, 1095, 1172, 1185, 1453, 1466, 1847, 1860
AlwNI CAGNNNCTG 1 cut(s) 2185
Aor13HI TCCGGA 2 cut(s) 508, 1699
AoxI GGCC 6 cut(s) 422, 481, 658, 1020, 1571, 2117
ApeKI GCWGC 3 cut(s) 707, 1565, 1676
ApoI RAATTY 2 cut(s) 1183, 1993
AsiGI ACCGGT 1 cut(s) 1471
Asp700I GAANNNNTTC 1 cut(s) 1225
Asp718I GGTACC 1 cut(s) 1445
AspS9I GGNCC 7 cut(s) 342, 423, 458, 941, 1076, 1499, 1586
AsuC2I CCSGG 5 cut(s) 287, 945, 1080, 1092, 1857
AsuHPI GGTGA 9 cut(s) 157, 970, 1256, 1316, 1778, 1826, 2149, 2220, 2230
AvaII GGWCC 6 cut(s) 342, 458, 941, 1076, 1499, 1586
BaeGI GKGCMC 1 cut(s) 1658
BaeI ACNNNNGTAYC 2 cut(s) 402, 435
BamHI GGATCC 4 cut(s) 1087, 1177, 1458, 1852
BanI GGYRCC 1 cut(s) 1445
BauI CACGAG 1 cut(s) 1494
BbrPI CACGTG 2 cut(s) 1330, 1663
BbvI GCAGC 3 cut(s) 694, 1552, 1663
BccI CCATC 8 cut(s) 270, 617, 709, 1133, 1597, 1642, 1771, 2267
BceAI ACGGC 5 cut(s) 645, 715, 788, 1440, 1758
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 1548, 2232
BcnI CCSGG 5 cut(s) 287, 945, 1080, 1092, 1857
BcoDI GTCTC 4 cut(s) 922, 1158, 1505, 2243
BfrI CTTAAG 1 cut(s) 1685
BfuAI ACCTGC 1 cut(s) 1474
BisI GCNGC 9 cut(s) 708, 738, 789, 1135, 1566, 1677, 1692, 1716, 2120
BlsI GCNGC 9 cut(s) 709, 739, 790, 1136, 1567, 1678, 1693, 1717, 2121
Bme1390I CCNGG 7 cut(s) 287, 945, 1080, 1092, 1548, 1857, 2232
Bme18I GGWCC 6 cut(s) 342, 458, 941, 1076, 1499, 1586
BmgBI CACGTC 2 cut(s) 94, 1987
BmgT120I GGNCC 7 cut(s) 342, 423, 458, 941, 1076, 1499, 1586
BmiI GGNNCC 8 cut(s) 720, 855, 943, 1089, 1179, 1447, 1460, 1854
BmrFI CCNGG 7 cut(s) 287, 945, 1080, 1092, 1548, 1857, 2232
BmsI GCATC 1 cut(s) 1490
BoxI GACNNNNGTC 3 cut(s) 246, 450, 986
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 1 cut(s) 1380
BpuMI CCSGG 5 cut(s) 287, 945, 1080, 1092, 1857
Bsa29I ATCGAT 2 cut(s) 408, 1005
BsaAI YACGTR 2 cut(s) 1330, 1663
BsaHI GRCGYC 4 cut(s) 677, 702, 846, 1514
BsaJI CCNNGG 5 cut(s) 211, 448, 974, 1740, 2298
BsaWI WCCGGW 7 cut(s) 508, 761, 1471, 1531, 1578, 1611, 1699
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 834, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 1455, 2126
Bse118I RCCGGY 4 cut(s) 656, 929, 1336, 1471
Bse1I ACTGG 4 cut(s) 143, 418, 1795, 2257
BseAI TCCGGA 2 cut(s) 508, 1699
BseBI CCWGG 2 cut(s) 1548, 2232
BseCI ATCGAT 2 cut(s) 408, 1005
BseDI CCNNGG 5 cut(s) 211, 448, 974, 1740, 2298
BseGI GGATG 9 cut(s) 262, 720, 984, 1281, 1394, 1417, 1540, 1696, 1786
BseLI CCNNNNNNNGG 3 cut(s) 474, 1455, 2126
BseMII CTCAG 4 cut(s) 315, 588, 1611, 2177
BseNI ACTGG 4 cut(s) 143, 418, 1795, 2257
BseRI GAGGAG 2 cut(s) 165, 1377
BseSI GKGCMC 1 cut(s) 1658
BseX3I CGGCCG 1 cut(s) 658
BseXI GCAGC 3 cut(s) 694, 1552, 1663
BseYI CCCAGC 1 cut(s) 710
BsgI GTGCAG 1 cut(s) 1452
Bsh1236I CGCG 1 cut(s) 613
Bsh1285I CGRYCG 1 cut(s) 661
BshFI GGCC 6 cut(s) 424, 483, 660, 1022, 1573, 2119
BshNI GGYRCC 1 cut(s) 1445
BshTI ACCGGT 1 cut(s) 1471
BshVI ATCGAT 2 cut(s) 408, 1005
BsiEI CGRYCG 1 cut(s) 661
BsiWI CGTACG 1 cut(s) 1252
BslFI GGGAC 5 cut(s) 646, 927, 1995, 2143, 2280
BslI CCNNNNNNNGG 3 cut(s) 474, 1455, 2126
BsmAI GTCTC 4 cut(s) 922, 1158, 1505, 2243
BsmBI CGTCTC 3 cut(s) 922, 1158, 1505
BsmFI GGGAC 5 cut(s) 646, 927, 1995, 2143, 2280
BsnI GGCC 6 cut(s) 424, 483, 660, 1022, 1573, 2119
Bsp1286I GDGCHC 1 cut(s) 1658
Bsp13I TCCGGA 2 cut(s) 508, 1699
Bsp143I GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
Bsp19I CCATGG 1 cut(s) 211
BspANI GGCC 6 cut(s) 424, 483, 660, 1022, 1573, 2119
BspCNI CTCAG 4 cut(s) 316, 589, 1612, 2178
BspDI ATCGAT 2 cut(s) 408, 1005
BspEI TCCGGA 2 cut(s) 508, 1699
BspFNI CGCG 1 cut(s) 613
BspLI GGNNCC 8 cut(s) 720, 855, 943, 1089, 1179, 1447, 1460, 1854
BspMI ACCTGC 1 cut(s) 1474
BspPI GGATC 9 cut(s) 396, 1082, 1095, 1172, 1185, 1453, 1466, 1847, 1860
BspT107I GGYRCC 1 cut(s) 1445
BspTI CTTAAG 1 cut(s) 1685
BsrBI CCGCTC 1 cut(s) 1979
BsrFI RCCGGY 4 cut(s) 656, 929, 1336, 1471
BsrI ACTGG 4 cut(s) 143, 418, 1795, 2257
BssAI RCCGGY 4 cut(s) 656, 929, 1336, 1471
BssECI CCNNGG 5 cut(s) 211, 448, 974, 1740, 2298
BssMI GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
BssNAI GTATAC 1 cut(s) 1069
BssNI GRCGYC 4 cut(s) 677, 702, 846, 1514
BssSI CACGAG 1 cut(s) 1494
BssT1I CCWWGG 2 cut(s) 211, 2298
Bst1107I GTATAC 1 cut(s) 1069
Bst2BI CACGAG 1 cut(s) 1494
Bst2UI CCWGG 2 cut(s) 1548, 2232
Bst6I CTCTTC 4 cut(s) 70, 175, 363, 2316
BstACI GRCGYC 4 cut(s) 677, 702, 846, 1514
BstAFI CTTAAG 1 cut(s) 1685
BstBAI YACGTR 2 cut(s) 1330, 1663
BstC8I GCNNGC 3 cut(s) 658, 1654, 1658
BstDEI CTNAG 7 cut(s) 256, 324, 338, 597, 784, 1620, 2186
BstDSI CCRYGG 4 cut(s) 211, 448, 974, 1740
BstF5I GGATG 9 cut(s) 262, 720, 984, 1281, 1394, 1417, 1540, 1696, 1786
BstFNI CGCG 1 cut(s) 613
BstKTI GATC 8 cut(s) 391, 1090, 1180, 1203, 1251, 1461, 1727, 1855
BstMAI GTCTC 4 cut(s) 922, 1158, 1505, 2243
BstMBI GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
BstMCI CGRYCG 1 cut(s) 661
BstMWI GCNNNNNNNGC 7 cut(s) 716, 725, 734, 884, 893, 1976, 2155
BstNI CCWGG 2 cut(s) 1548, 2232
BstPAI GACNNNNGTC 3 cut(s) 246, 450, 986
BstSCI CCNGG 7 cut(s) 285, 943, 1078, 1090, 1546, 1855, 2230
BstSLI GKGCMC 1 cut(s) 1658
BstUI CGCG 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 694, 1552, 1663
BstX2I RGATCY 5 cut(s) 388, 1087, 1177, 1458, 1852
BstXI CCANNNNNNTGG 1 cut(s) 1849
BstYI RGATCY 5 cut(s) 388, 1087, 1177, 1458, 1852
BstZ17I GTATAC 1 cut(s) 1069
BstZI CGGCCG 1 cut(s) 658
Bsu15I ATCGAT 2 cut(s) 408, 1005
BsuRI GGCC 6 cut(s) 424, 483, 660, 1022, 1573, 2119
BsuTUI ATCGAT 2 cut(s) 408, 1005
BtgI CCRYGG 4 cut(s) 211, 448, 974, 1740
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 2 cut(s) 94, 1987
BtsCI GGATG 9 cut(s) 262, 720, 984, 1281, 1394, 1417, 1540, 1696, 1786
BtsIMutI CAGTG 2 cut(s) 136, 1788
BveI ACCTGC 1 cut(s) 1474
Cac8I GCNNGC 3 cut(s) 658, 1654, 1658
CaiI CAGNNNCTG 1 cut(s) 2185
Cfr10I RCCGGY 4 cut(s) 656, 929, 1336, 1471
Cfr13I GGNCC 7 cut(s) 342, 423, 458, 941, 1076, 1499, 1586
ClaI ATCGAT 2 cut(s) 408, 1005
CseI GACGC 6 cut(s) 251, 666, 691, 835, 878, 1522
CsiI ACCWGGT 1 cut(s) 1546
Csp6I GTAC 4 cut(s) 142, 1102, 1253, 1446
CspAI ACCGGT 1 cut(s) 1471
CviAII CATG 4 cut(s) 212, 269, 629, 1829
CviQI GTAC 4 cut(s) 142, 1102, 1253, 1446
DdeI CTNAG 7 cut(s) 256, 324, 338, 597, 784, 1620, 2186
DpnI GATC 8 cut(s) 390, 1089, 1179, 1202, 1250, 1460, 1726, 1854
DpnII GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
DraI TTTAAA 1 cut(s) 1998
EaeI YGGCCR 2 cut(s) 658, 1020
EagI CGGCCG 1 cut(s) 658
Eam1104I CTCTTC 4 cut(s) 70, 175, 363, 2316
EarI CTCTTC 4 cut(s) 70, 175, 363, 2316
EciI GGCGGA 2 cut(s) 643, 916
EclXI CGGCCG 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 211, 2298
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 6 cut(s) 342, 458, 941, 1076, 1499, 1586
Eco52I CGGCCG 1 cut(s) 658
Eco72I CACGTG 2 cut(s) 1330, 1663
EcoO109I RGGNCCY 1 cut(s) 941
EcoRII CCWGG 2 cut(s) 1546, 2230
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 2 cut(s) 211, 2298
ErhI CCWWGG 2 cut(s) 211, 2298
Esp3I CGTCTC 3 cut(s) 922, 1158, 1505
FaeI CATG 4 cut(s) 215, 272, 632, 1832
FaqI GGGAC 5 cut(s) 646, 927, 1995, 2143, 2280
FatI CATG 4 cut(s) 211, 268, 628, 1828
FauI CCCGC 4 cut(s) 349, 1113, 1665, 1972
FblI GTMKAC 4 cut(s) 96, 361, 1068, 2051
Fnu4HI GCNGC 9 cut(s) 708, 738, 789, 1135, 1566, 1677, 1692, 1716, 2120
FokI GGATG 9 cut(s) 249, 727, 991, 1288, 1381, 1404, 1547, 1683, 1793
Fsp4HI GCNGC 9 cut(s) 708, 738, 789, 1135, 1566, 1677, 1692, 1716, 2120
GluI GCNGC 9 cut(s) 708, 738, 789, 1135, 1566, 1677, 1692, 1716, 2120
GsaI CCCAGC 1 cut(s) 714
GsuI CTGGAG 1 cut(s) 1380
HaeIII GGCC 6 cut(s) 424, 483, 660, 1022, 1573, 2119
HgaI GACGC 6 cut(s) 251, 666, 691, 835, 878, 1522
Hin1I GRCGYC 4 cut(s) 677, 702, 846, 1514
Hin1II CATG 4 cut(s) 215, 272, 632, 1832
HincII GTYRAC 4 cut(s) 97, 251, 681, 2052
HindII GTYRAC 4 cut(s) 97, 251, 681, 2052
HphI GGTGA 9 cut(s) 157, 970, 1256, 1316, 1778, 1826, 2149, 2220, 2230
Hpy188III TCNNGA 9 cut(s) 31, 509, 1085, 1163, 1230, 1359, 1508, 1700, 1990
Hpy99I CGWCG 8 cut(s) 98, 539, 707, 815, 851, 1950, 1991, 2011
HpyAV CCTTC 4 cut(s) 57, 1160, 1186, 1301
HpyCH4IV ACGT 5 cut(s) 93, 666, 1329, 1662, 1986
HpyCH4V TGCA 4 cut(s) 307, 1469, 1481, 1970
HpyF10VI GCNNNNNNNGC 7 cut(s) 716, 725, 734, 884, 893, 1976, 2155
HpyF3I CTNAG 7 cut(s) 256, 324, 338, 597, 784, 1620, 2186
HpySE526I ACGT 5 cut(s) 93, 666, 1329, 1662, 1986
Hsp92I GRCGYC 4 cut(s) 677, 702, 846, 1514
Hsp92II CATG 4 cut(s) 215, 272, 632, 1832
Kpn2I TCCGGA 2 cut(s) 508, 1699
KpnI GGTACC 1 cut(s) 1449
KroI GCCGGC 1 cut(s) 656
KroNI GCCGGC 1 cut(s) 658
Kzo9I GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
LmnI GCTCC 7 cut(s) 539, 724, 884, 893, 976, 992, 2234
Lsp1109I GCAGC 3 cut(s) 694, 1552, 1663
LweI GCATC 1 cut(s) 1490
MabI ACCWGGT 1 cut(s) 1546
MaeII ACGT 5 cut(s) 93, 666, 1329, 1662, 1986
MalI GATC 8 cut(s) 390, 1089, 1179, 1202, 1250, 1460, 1726, 1854
MbiI CCGCTC 1 cut(s) 1979
MboI GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
MflI RGATCY 5 cut(s) 388, 1087, 1177, 1458, 1852
MhlI GDGCHC 1 cut(s) 1658
MluCI AATT 4 cut(s) 1183, 1225, 1906, 1993
MmeI TCCRAC 8 cut(s) 366, 517, 734, 820, 970, 1155, 1445, 1486
MroI TCCGGA 2 cut(s) 508, 1699
MroNI GCCGGC 1 cut(s) 656
MroXI GAANNNNTTC 1 cut(s) 1225
MseI TTAA 7 cut(s) 471, 953, 1527, 1686, 1997, 2109, 2205
MslI CAYNNNNRTG 3 cut(s) 267, 1646, 2216
MspA1I CMGCKG 1 cut(s) 1764
MspCI CTTAAG 1 cut(s) 1685
MspR9I CCNGG 7 cut(s) 287, 945, 1080, 1092, 1548, 1857, 2232
MvaI CCWGG 2 cut(s) 1548, 2232
MvnI CGCG 1 cut(s) 613
MwoI GCNNNNNNNGC 7 cut(s) 716, 725, 734, 884, 893, 1976, 2155
NaeI GCCGGC 1 cut(s) 658
NciI CCSGG 5 cut(s) 287, 945, 1080, 1092, 1857
NcoI CCATGG 1 cut(s) 211
NdeII GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
NgoMIV GCCGGC 1 cut(s) 656
NlaIII CATG 4 cut(s) 215, 272, 632, 1832
NlaIV GGNNCC 8 cut(s) 720, 855, 943, 1089, 1179, 1447, 1460, 1854
NmeAIII GCCGAG 1 cut(s) 666
OliI CACNNNNGTG 1 cut(s) 2216
PcsI WCGNNNNNNNCGW 7 cut(s) 93, 99, 405, 610, 699, 801, 1178
PdiI GCCGGC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 1225
PfeI GAWTC 7 cut(s) 546, 1221, 1372, 1391, 1522, 2087, 2093
Pfl23II CGTACG 1 cut(s) 1252
PflFI GACNNNGTC 2 cut(s) 815, 1080
PflMI CCANNNNNTGG 1 cut(s) 474
PfoI TCCNGGA 2 cut(s) 285, 1855
PinAI ACCGGT 1 cut(s) 1471
PkrI GCNGC 9 cut(s) 709, 739, 790, 1136, 1567, 1678, 1693, 1717, 2121
PmaCI CACGTG 2 cut(s) 1330, 1663
PmlI CACGTG 2 cut(s) 1330, 1663
Ppu21I YACGTR 2 cut(s) 1330, 1663
PpuMI RGGWCCY 1 cut(s) 941
PshAI GACNNNNGTC 3 cut(s) 246, 450, 986
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 1546, 2230
PspCI CACGTG 2 cut(s) 1330, 1663
PspFI CCCAGC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 1546, 2230
PspLI CGTACG 1 cut(s) 1252
PspN4I GGNNCC 8 cut(s) 720, 855, 943, 1089, 1179, 1447, 1460, 1854
PspPI GGNCC 7 cut(s) 342, 423, 458, 941, 1076, 1499, 1586
PspPPI RGGWCCY 1 cut(s) 941
PstNI CAGNNNCTG 1 cut(s) 2185
PsuI RGATCY 5 cut(s) 388, 1087, 1177, 1458, 1852
PsyI GACNNNGTC 2 cut(s) 815, 1080
RsaI GTAC 4 cut(s) 143, 1103, 1254, 1447
RsaNI GTAC 4 cut(s) 142, 1102, 1253, 1446
RseI CAYNNNNRTG 3 cut(s) 267, 1646, 2216
SalI GTCGAC 2 cut(s) 95, 2050
SaqAI TTAA 7 cut(s) 471, 953, 1527, 1686, 1997, 2109, 2205
SatI GCNGC 9 cut(s) 708, 738, 789, 1135, 1566, 1677, 1692, 1716, 2120
Sau3AI GATC 8 cut(s) 388, 1087, 1177, 1200, 1248, 1458, 1724, 1852
Sau96I GGNCC 7 cut(s) 342, 423, 458, 941, 1076, 1499, 1586
ScrFI CCNGG 7 cut(s) 287, 945, 1080, 1092, 1548, 1857, 2232
SduI GDGCHC 1 cut(s) 1658
SexAI ACCWGGT 1 cut(s) 1546
SfaNI GCATC 1 cut(s) 1490
SinI GGWCC 6 cut(s) 342, 458, 941, 1076, 1499, 1586
SmiMI CAYNNNNRTG 3 cut(s) 267, 1646, 2216
SmlI CTYRAG 1 cut(s) 1685
SmoI CTYRAG 1 cut(s) 1685
Sse9I AATT 4 cut(s) 1183, 1225, 1906, 1993
SspI AATATT 1 cut(s) 1558
StyD4I CCNGG 7 cut(s) 285, 943, 1078, 1090, 1546, 1855, 2230
StyI CCWWGG 2 cut(s) 211, 2298
TaiI ACGT 5 cut(s) 96, 669, 1332, 1665, 1989
TasI AATT 4 cut(s) 1183, 1225, 1906, 1993
TauI GCSGC 6 cut(s) 740, 791, 1137, 1694, 1718, 2122
TfiI GAWTC 7 cut(s) 546, 1221, 1372, 1391, 1522, 2087, 2093
Tru1I TTAA 7 cut(s) 471, 953, 1527, 1686, 1997, 2109, 2205
Tru9I TTAA 7 cut(s) 471, 953, 1527, 1686, 1997, 2109, 2205
TscAI CASTG 2 cut(s) 143, 1795
TseI GCWGC 3 cut(s) 707, 1565, 1676
TspDTI ATGAA 5 cut(s) 579, 599, 603, 1398, 2050
TspGWI ACGGA 6 cut(s) 287, 826, 951, 963, 1270, 2296
TspRI CASTG 2 cut(s) 143, 1795
Tth111I GACNNNGTC 2 cut(s) 815, 1080
Van91I CCANNNNNTGG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 1685
VpaK11BI GGWCC 6 cut(s) 342, 458, 941, 1076, 1499, 1586
XapI RAATTY 2 cut(s) 1183, 1993
XbaI TCTAGA 1 cut(s) 1229
XmiI GTMKAC 4 cut(s) 96, 361, 1068, 2051
XmnI GAANNNNTTC 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.