Prupe.3G032200_v2.0.a1

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0017066
Physical Location & Seq
Forward (+)
2394192 .. 2397245
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G032200.1

Sequence Viewer

Length: 2355 bp
ATGGCTATCTCTCTTCTCTATCTCCTCATAATCTCACTCTCCTTCTCCAACTTACCCTCTTCTTCTTCTTCTTCTGATAATAATAATAATAATGTTAATAATGCTGCAAAGACCTTCATAGTCCAAGTCCAGCCAAGCTCCAAACCCTCCATTTTCCCAACCCACCAAGACTGGTACTCTTCCTCTCTCTCCTCTCTTTCTTCTGATAAAGCCACTGCCCCCACCGTCCTCCACACTTACTCTACTGTCTTCCATGGCTTCTCTGCCAAGCTTTCCCCATCCCAAGCCCAGACACTTCAGTCCCTTGACCACGTCACCGCCATTATCCCCGAGCAAGTCCGCCAACTCCACACCACCCGCTCCCCCGAGTTCCTCGGCCTCCGCTCCACCGACGCCGCCGGTACCCTCCTCAGGGAGTCTGATTTCGGGTCTGACCTTGTCATCGGAGTCATCGACACCGGCATCTGGCCCGAACGCAAGTCATTCCACGACCGAGACCTGGGCCCCACCCCCTCCAAGTGGAAAGGCCAGTGTGTCGCCGGCAAAGACTTCCCCGCCACCATCTGCAACCGCAAGCTTATCGGCGCTCGCTTCTTCTCCGCCGGCTTCGAGTCCACGAACGGAAAAATGAATGAGACCTCAGAGTACCGCTCCCCCCGCGACTCCGACGGCCACGGGACCCACACTGCCTCCATCGCTGCCGGGAGGTACGTTTTCCCAGCGTCCACTCTCGGCTACGCCAAGGGCGTTGCCGCCGGAATGGCTCCCAAGGCCCGGCTTGCCGCCTACAAGGTCTGCTGGAGTGCCGGCTGCTACGACTCCGACATCCTCGCCGCGTTCGACGCCGCCGTCGCAGACGGCTGCGACGTCGTTTCCCTCAGCGTCGGTGGCGTCGTCGTCCCCTACCACCTCGACGCCATAGCCATCGGCGCGTACGGAGCCTCGGACTCCGGCGTCTTCGTCTCTGCCTCCGCCGGAAACGGCGGTCCCGGAGGGCTCACTGTCACCAATGTGGCACCGTGGGTGACAACCGTCGGCGCGGGAACTATCGACAGGGACTTCCCGGCCGATGTGAAGCTCGGGAACGGCAGAATAATCCCGGGCATGAGCATCTATTCCGGGCCGGGTCTCGCTCCGGGTCGGATGTACCCGCTGGTTTACGCGGGCGGCGTCGGCGGCGATGGCTACTCCTCGTCTCTGTGTCTAGAAGGTTCTCTGAGTCAAGTAAAAGGGAAAATCGTCGTCTGCGACAGAGGAATTAATTCAAGAGCCGCGAAAGGTGACGTGGTGAAGAAAGCCGGAGGCGTTGGTATGATACTCGCGAACGGGGTTTTCGACGGCGAAGGACTAGTAGCTGACTGCCACGTGTTACCCGCCACCGCTGTGGCTGCCTCCACGGGCGACGAAATCAGGAGATACATCGCCGCATCCAAGTCTAAGTCCCCAGCTACAGCGACAATTGTGTTCAAGGGGACTCGGATCCGGGTCCGGCCCGCGCCGGTCGTGGCGTCGTTTTCAGCCCGCGGGCCGAATCCGGAGTCTCCGGAGATATTGAAGCCGGATGTTATCGCGCCCGGCTTGAATATATTGGCGGCTTGGCCCGATAAAGTCGGGCCCTCAGGCGTGGCTTCGGATAAGAGAAACACGGAGTTTAATATCCTCTCGGGAACATCAATGGCTTGCCCACACGTGTCCGGCTTGGCGGCGCTGTTGAAAGCGGCGCATCCAGATTGGAGCCCGGCCGCCATTAGATCCGCCCTGATGACCACGGCATACACCGTGGACAATCGGGGTGAGACCATGCTTGACGAGTCCAGCGGCAACACTTCCAGCGTTATGGACTTCGGGGCGGGTCATGTCCACCCGCAAAAGGCAATGGACCCGGGGTTGGTCTATGACATTGTCTCTTACGATTACGTGGATTTCTTGTGCAATTCCAATTATACCACTAAGAACATCCAGGTGGTGACTAGGAAGTTTGCCAATTGCAATGGGGCTAAGAGGGCAGGGCATGCTGGCAACTTAAATTACCCCTCCTTGTCTGTGGTGTTTCAGCAGTATGGAAAGCATAAGATGTCTACTCATTTTATTCGGACCGTGACAAATGTGGGTACGCCGAATTCGGTTTATCAGGTGACGGTTAAGCCGGCTGCAGGGATGACGGTGACAGTGGAGCCGGAGAAGCTTGCGTTTAGGAGGGTGGGGCAGAAGTTAAGTTTTCTTGTGAGGGTGCAAGCCTTGGCAGTGAAGCTATCCCCTGGTAGCACTAGCGTGAAGAGTGGTTCTATAGTTTGGTCTGATGGGAAGCACACTGTGACTAGCCCCTTGGTTGTGACAATGCAACAACCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

785

Amino Acids

82.24

Weight (kDa)

9.0

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 298, 848, 953
AatII GACGTC 1 cut(s) 870
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 2 cut(s) 401, 1015
AccBSI CCGCTC 3 cut(s) 360, 384, 651
AccI GTMKAC 1 cut(s) 2078
AccIII TCCGGA 2 cut(s) 1534, 1543
AclWI GGATC 3 cut(s) 1474, 1487, 1746
AcoI YGGCCR 3 cut(s) 670, 1065, 1740
AcsI RAATTY 1 cut(s) 2119
AcuI CTGAAG 1 cut(s) 281
AcvI CACGTG 2 cut(s) 1366, 1690
AcyI GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
AdeI CACNNNGTG 1 cut(s) 2314
AfaI GTAC 7 cut(s) 176, 403, 647, 710, 935, 1148, 2113
AfiI CCNNNNNNNGG 9 cut(s) 411, 412, 465, 499, 519, 774, 1870, 1888, 2153
AflIII ACRYGT 3 cut(s) 1365, 1687, 1689
AgsI TTSAA 5 cut(s) 1266, 1468, 1555, 1582, 1714
AhlI ACTAGT 1 cut(s) 1348
AjiI CACGTC 2 cut(s) 313, 1285
AjnI CCWGG 3 cut(s) 498, 1959, 2257
AleI CACNNNNGTG 3 cut(s) 1010, 1382, 2270
AluBI AGCT 8 cut(s) 138, 271, 577, 1078, 1355, 1448, 2185, 2251
AluI AGCT 8 cut(s) 138, 271, 577, 1078, 1355, 1448, 2185, 2251
Alw26I GTCTC 8 cut(s) 489, 629, 967, 1133, 1200, 1545, 1790, 1909
AlwI GGATC 3 cut(s) 1474, 1487, 1746
Ama87I CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
Aor13HI TCCGGA 2 cut(s) 1534, 1543
ApaI GGGCCC 2 cut(s) 506, 1617
ApeKI GCWGC 6 cut(s) 104, 698, 810, 861, 1388, 2150
ApoI RAATTY 1 cut(s) 2119
ArsI GACNNNNNNTTYG 2 cut(s) 1523, 1555
AseI ATTAAT 1 cut(s) 1260
Asp700I GAANNNNTTC 1 cut(s) 1261
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 7 cut(s) 587, 932, 1040, 1498, 1573, 1708, 1723
AsuHPI GGTGA 9 cut(s) 307, 997, 1036, 1292, 1300, 1805, 1978, 2146, 2176
AvaI CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
AvaII GGWCC 5 cut(s) 678, 986, 1486, 1879, 2094
AxyI CCTNAGG 2 cut(s) 410, 1618
BaeGI GKGCMC 2 cut(s) 506, 1617
BamHI GGATCC 1 cut(s) 1479
BanI GGYRCC 2 cut(s) 401, 1015
BanII GRGCYC 4 cut(s) 506, 999, 1617, 1739
BbrPI CACGTG 2 cut(s) 1366, 1690
BbsI GAAGAC 2 cut(s) 241, 949
BbvCI CCTCAGC 1 cut(s) 878
BbvI GCAGC 6 cut(s) 91, 685, 797, 848, 1375, 2137
BccI CCATC 6 cut(s) 286, 569, 701, 932, 1175, 2294
BceAI ACGGC 7 cut(s) 685, 833, 874, 997, 1102, 1354, 1785
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BciT130I CCWGG 3 cut(s) 500, 1961, 2259
BcoDI GTCTC 8 cut(s) 489, 629, 967, 1133, 1200, 1545, 1790, 1909
BcuI ACTAGT 1 cut(s) 1348
BfaI CTAG 6 cut(s) 1205, 1349, 1971, 2268, 2319, 2353
BfmI CTRYAG 3 cut(s) 1449, 2151, 2286
BfoI RGCGCY 2 cut(s) 588, 1709
BglI GCCNNNNNGGC 1 cut(s) 761
Bme18I GGWCC 5 cut(s) 678, 986, 1486, 1879, 2094
BmeT110I CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
BmgBI CACGTC 2 cut(s) 313, 1285
BmsI GCATC 4 cut(s) 471, 1119, 1436, 1732
BoxI GACNNNNGTC 1 cut(s) 1031
BpiI GAAGAC 2 cut(s) 241, 949
BplI GAGNNNNNCTC 2 cut(s) 635, 667
BpmI CTGGAG 1 cut(s) 820
Bpu10I CCTNAGC 1 cut(s) 878
BsaAI YACGTR 3 cut(s) 1366, 1690, 1918
BsaHI GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
BsaI GGTCTC 4 cut(s) 489, 629, 1133, 1790
BsaWI WCCGGW 2 cut(s) 1534, 1543
BsaXI ACNNNNNCTCC 2 cut(s) 674, 704
Bsc4I CCNNNNNNNGG 9 cut(s) 411, 412, 465, 499, 519, 774, 1870, 1888, 2153
Bse118I RCCGGY 7 cut(s) 398, 458, 539, 602, 806, 1498, 2146
Bse1I ACTGG 2 cut(s) 176, 529
Bse21I CCTNAGG 2 cut(s) 410, 1618
Bse3DI GCAATG 2 cut(s) 1881, 1996
BseAI TCCGGA 2 cut(s) 1534, 1543
BseBI CCWGG 3 cut(s) 500, 1961, 2259
BseGI GGATG 8 cut(s) 278, 825, 1149, 1427, 1567, 1723, 1956, 2163
BseLI CCNNNNNNNGG 9 cut(s) 411, 412, 465, 499, 519, 774, 1870, 1888, 2153
BseMI GCAATG 2 cut(s) 1881, 1996
BseMII CTCAG 5 cut(s) 424, 654, 892, 1208, 1632
BseNI ACTGG 2 cut(s) 176, 529
BseRI GAGGAG 4 cut(s) 14, 181, 398, 1180
BseSI GKGCMC 2 cut(s) 506, 1617
BseX3I CGGCCG 2 cut(s) 1065, 1740
BseXI GCAGC 6 cut(s) 91, 685, 797, 848, 1375, 2137
BseYI CCCAGC 2 cut(s) 718, 1444
Bsh1285I CGRYCG 4 cut(s) 493, 1068, 1503, 1743
BshNI GGYRCC 2 cut(s) 401, 1015
BsiEI CGRYCG 4 cut(s) 493, 1068, 1503, 1743
BsiHKCI CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
BsiWI CGTACG 1 cut(s) 933
BslFI GGGAC 7 cut(s) 286, 691, 884, 972, 1070, 1426, 1486
BslI CCNNNNNNNGG 9 cut(s) 411, 412, 465, 499, 519, 774, 1870, 1888, 2153
BsmAI GTCTC 8 cut(s) 489, 629, 967, 1133, 1200, 1545, 1790, 1909
BsmBI CGTCTC 2 cut(s) 967, 1200
BsmFI GGGAC 7 cut(s) 286, 691, 884, 972, 1070, 1426, 1486
Bso31I GGTCTC 4 cut(s) 489, 629, 1133, 1790
BsoBI CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
Bsp120I GGGCCC 2 cut(s) 502, 1613
Bsp1286I GDGCHC 4 cut(s) 506, 999, 1617, 1739
Bsp13I TCCGGA 2 cut(s) 1534, 1543
Bsp143I GATC 2 cut(s) 1479, 1751
Bsp19I CCATGG 1 cut(s) 253
Bsp68I TCGCGA 1 cut(s) 1322
BspCNI CTCAG 5 cut(s) 423, 653, 891, 1209, 1631
BspEI TCCGGA 2 cut(s) 1534, 1543
BspMAI CTGCAG 1 cut(s) 2155
BspPI GGATC 3 cut(s) 1474, 1487, 1746
BspT107I GGYRCC 2 cut(s) 401, 1015
BspTNI GGTCTC 4 cut(s) 489, 629, 1133, 1790
BsrBI CCGCTC 3 cut(s) 360, 384, 651
BsrDI GCAATG 2 cut(s) 1881, 1996
BsrFI RCCGGY 7 cut(s) 398, 458, 539, 602, 806, 1498, 2146
BsrI ACTGG 2 cut(s) 176, 529
BssAI RCCGGY 7 cut(s) 398, 458, 539, 602, 806, 1498, 2146
BssMI GATC 2 cut(s) 1479, 1751
BssNI GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
BssT1I CCWWGG 5 cut(s) 253, 741, 768, 2238, 2325
Bst2UI CCWGG 3 cut(s) 500, 1961, 2259
Bst6I CTCTTC 4 cut(s) 18, 64, 184, 2270
BstACI GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
BstAPI GCANNNNNTGC 1 cut(s) 2012
BstBAI YACGTR 3 cut(s) 1366, 1690, 1918
BstDEI CTNAG 8 cut(s) 410, 640, 878, 1217, 1437, 1618, 1950, 1998
BstDSI CCRYGG 7 cut(s) 253, 673, 1019, 1395, 1522, 1767, 1779
BstF5I GGATG 8 cut(s) 278, 825, 1149, 1427, 1567, 1723, 1956, 2163
BstH2I RGCGCY 2 cut(s) 588, 1709
BstHHI GCGC 7 cut(s) 587, 932, 1040, 1498, 1573, 1708, 1723
BstKTI GATC 2 cut(s) 1482, 1754
BstMAI GTCTC 8 cut(s) 489, 629, 967, 1133, 1200, 1545, 1790, 1909
BstMBI GATC 2 cut(s) 1479, 1751
BstMCI CGRYCG 4 cut(s) 493, 1068, 1503, 1743
BstNI CCWGG 3 cut(s) 500, 1961, 2259
BstNSI RCATGY 1 cut(s) 2015
BstPAI GACNNNNGTC 1 cut(s) 1031
BstSFI CTRYAG 3 cut(s) 1449, 2151, 2286
BstSLI GKGCMC 2 cut(s) 506, 1617
BstV1I GCAGC 6 cut(s) 91, 685, 797, 848, 1375, 2137
BstV2I GAAGAC 2 cut(s) 241, 949
BstX2I RGATCY 2 cut(s) 1479, 1751
BstXI CCANNNNNNTGG 2 cut(s) 1384, 1837
BstYI RGATCY 2 cut(s) 1479, 1751
BstZI CGGCCG 2 cut(s) 1065, 1740
Bsu36I CCTNAGG 2 cut(s) 410, 1618
BtgI CCRYGG 7 cut(s) 253, 673, 1019, 1395, 1522, 1767, 1779
BtgZI GCGATG 3 cut(s) 679, 1194, 1405
BtrI CACGTC 2 cut(s) 313, 1285
BtsCI GGATG 8 cut(s) 278, 825, 1149, 1427, 1567, 1723, 1956, 2163
BtsI GCAGTG 3 cut(s) 213, 684, 2250
BtsIMutI CAGTG 7 cut(s) 213, 536, 684, 999, 2175, 2250, 2310
BtuMI TCGCGA 1 cut(s) 1322
CfoI GCGC 7 cut(s) 587, 932, 1040, 1498, 1573, 1708, 1723
Cfr10I RCCGGY 7 cut(s) 398, 458, 539, 602, 806, 1498, 2146
Cfr42I CCGCGG 1 cut(s) 1525
Cfr9I CCCGGG 2 cut(s) 1099, 1882
CpoI CGGWCCG 1 cut(s) 2094
CseI GACGC 9 cut(s) 401, 711, 851, 871, 880, 923, 943, 1159, 1497
Csp6I GTAC 7 cut(s) 175, 402, 646, 709, 934, 1147, 2112
CspI CGGWCCG 1 cut(s) 2094
CviAII CATG 5 cut(s) 254, 1105, 1801, 1856, 2012
CviQI GTAC 7 cut(s) 175, 402, 646, 709, 934, 1147, 2112
DdeI CTNAG 8 cut(s) 410, 640, 878, 1217, 1437, 1618, 1950, 1998
DpnI GATC 2 cut(s) 1481, 1753
DpnII GATC 2 cut(s) 1479, 1751
DraIII CACNNNGTG 1 cut(s) 2314
DrdI GACNNNNNNGTC 3 cut(s) 298, 848, 953
DseDI GACNNNNNNGTC 3 cut(s) 298, 848, 953
EaeI YGGCCR 3 cut(s) 670, 1065, 1740
EagI CGGCCG 2 cut(s) 1065, 1740
Eam1104I CTCTTC 4 cut(s) 18, 64, 184, 2270
EarI CTCTTC 4 cut(s) 18, 64, 184, 2270
EciI GGCGGA 4 cut(s) 329, 589, 961, 1744
EclXI CGGCCG 2 cut(s) 1065, 1740
Eco130I CCWWGG 5 cut(s) 253, 741, 768, 2238, 2325
Eco24I GRGCYC 4 cut(s) 506, 999, 1617, 1739
Eco31I GGTCTC 4 cut(s) 489, 629, 1133, 1790
Eco47I GGWCC 5 cut(s) 678, 986, 1486, 1879, 2094
Eco52I CGGCCG 2 cut(s) 1065, 1740
Eco57I CTGAAG 1 cut(s) 281
Eco72I CACGTG 2 cut(s) 1366, 1690
Eco81I CCTNAGG 2 cut(s) 410, 1618
Eco88I CYCGRG 6 cut(s) 329, 365, 1079, 1099, 1663, 1882
EcoO109I RGGNCCY 3 cut(s) 503, 678, 1614
EcoRI GAATTC 1 cut(s) 2119
EcoRII CCWGG 3 cut(s) 498, 1959, 2257
EcoT14I CCWWGG 5 cut(s) 253, 741, 768, 2238, 2325
EcoT38I GRGCYC 4 cut(s) 506, 999, 1617, 1739
ErhI CCWWGG 5 cut(s) 253, 741, 768, 2238, 2325
Esp3I CGTCTC 2 cut(s) 967, 1200
FaeI CATG 5 cut(s) 257, 1108, 1804, 1859, 2015
FaqI GGGAC 7 cut(s) 286, 691, 884, 972, 1070, 1426, 1486
FatI CATG 5 cut(s) 253, 1104, 1800, 1855, 2011
FblI GTMKAC 1 cut(s) 2078
FokI GGATG 8 cut(s) 265, 812, 1156, 1414, 1574, 1710, 1943, 2170
FriOI GRGCYC 4 cut(s) 506, 999, 1617, 1739
FspBI CTAG 6 cut(s) 1205, 1349, 1971, 2268, 2319, 2353
GlaI GCGC 7 cut(s) 586, 931, 1039, 1497, 1572, 1707, 1722
GsaI CCCAGC 2 cut(s) 722, 1448
GsuI CTGGAG 1 cut(s) 820
HaeII RGCGCY 2 cut(s) 588, 1709
HgaI GACGC 9 cut(s) 401, 711, 851, 871, 880, 923, 943, 1159, 1497
HhaI GCGC 7 cut(s) 587, 932, 1040, 1498, 1573, 1708, 1723
Hin1I GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
Hin1II CATG 5 cut(s) 257, 1108, 1804, 1859, 2015
Hin6I GCGC 7 cut(s) 585, 930, 1038, 1496, 1571, 1706, 1721
HinP1I GCGC 7 cut(s) 585, 930, 1038, 1496, 1571, 1706, 1721
HindIII AAGCTT 3 cut(s) 269, 575, 2183
HphI GGTGA 9 cut(s) 307, 997, 1036, 1292, 1300, 1805, 1978, 2146, 2176
Hpy166II GTNNAC 6 cut(s) 615, 726, 1159, 1783, 1861, 2079
Hpy188III TCNNGA 9 cut(s) 1081, 1205, 1266, 1321, 1411, 1535, 1544, 1665, 1727
Hpy8I GTNNAC 6 cut(s) 615, 726, 1159, 1783, 1861, 2079
HpyAV CCTTC 4 cut(s) 52, 124, 1202, 1337
HpyCH4IV ACGT 7 cut(s) 312, 711, 867, 1284, 1365, 1689, 1917
HpyCH4V TGCA 7 cut(s) 107, 567, 1932, 1989, 2153, 2233, 2341
HpyF3I CTNAG 8 cut(s) 410, 640, 878, 1217, 1437, 1618, 1950, 1998
HpySE526I ACGT 7 cut(s) 312, 711, 867, 1284, 1365, 1689, 1917
Hsp92I GRCGYC 8 cut(s) 393, 843, 867, 891, 915, 954, 1170, 1508
Hsp92II CATG 5 cut(s) 257, 1108, 1804, 1859, 2015
HspAI GCGC 7 cut(s) 585, 930, 1038, 1496, 1571, 1706, 1721
KflI GGGWCCC 1 cut(s) 678
Kpn2I TCCGGA 2 cut(s) 1534, 1543
KpnI GGTACC 1 cut(s) 405
KroI GCCGGC 4 cut(s) 539, 602, 806, 2146
KroNI GCCGGC 4 cut(s) 541, 604, 808, 2148
KspI CCGCGG 1 cut(s) 1525
Kzo9I GATC 2 cut(s) 1479, 1751
LmnI GCTCC 9 cut(s) 143, 365, 389, 656, 769, 938, 1138, 1734, 2173
Lsp1109I GCAGC 6 cut(s) 91, 685, 797, 848, 1375, 2137
LweI GCATC 4 cut(s) 471, 1119, 1436, 1732
MaeI CTAG 6 cut(s) 1205, 1349, 1971, 2268, 2319, 2353
MaeII ACGT 7 cut(s) 312, 711, 867, 1284, 1365, 1689, 1917
MalI GATC 2 cut(s) 1481, 1753
MbiI CCGCTC 3 cut(s) 360, 384, 651
MboI GATC 2 cut(s) 1479, 1751
MfeI CAATTG 2 cut(s) 1458, 1984
MflI RGATCY 2 cut(s) 1479, 1751
MhlI GDGCHC 4 cut(s) 506, 999, 1617, 1739
MluCI AATT 8 cut(s) 1257, 1261, 1458, 1933, 1939, 1984, 2026, 2119
MmeI TCCRAC 4 cut(s) 72, 690, 846, 1121
MroI TCCGGA 2 cut(s) 1534, 1543
MroNI GCCGGC 4 cut(s) 539, 602, 806, 2146
MroXI GAANNNNTTC 1 cut(s) 1261
MseI TTAA 6 cut(s) 96, 1260, 1653, 2024, 2142, 2213
MslI CAYNNNNRTG 4 cut(s) 1010, 1382, 1961, 2270
MspA1I CMGCKG 4 cut(s) 1153, 1382, 1524, 1818
MunI CAATTG 2 cut(s) 1458, 1984
MvaI CCWGG 3 cut(s) 500, 1961, 2259
NaeI GCCGGC 4 cut(s) 541, 604, 808, 2148
NcoI CCATGG 1 cut(s) 253
NdeII GATC 2 cut(s) 1479, 1751
NgoMIV GCCGGC 4 cut(s) 539, 602, 806, 2146
NlaIII CATG 5 cut(s) 257, 1108, 1804, 1859, 2015
NmeAIII GCCGAG 2 cut(s) 354, 711
NruI TCGCGA 1 cut(s) 1322
NspI RCATGY 1 cut(s) 2015
OliI CACNNNNGTG 3 cut(s) 1010, 1382, 2270
PaeI GCATGC 1 cut(s) 2015
PcsI WCGNNNNNNNCGW 9 cut(s) 450, 744, 837, 846, 864, 891, 1167, 1245, 1332
PdiI GCCGGC 4 cut(s) 541, 604, 808, 2148
PdmI GAANNNNTTC 1 cut(s) 1261
PfeI GAWTC 1 cut(s) 1531
Pfl23II CGTACG 1 cut(s) 933
PflFI GACNNNGTC 3 cut(s) 311, 437, 1901
PfoI TCCNGGA 1 cut(s) 988
PmaCI CACGTG 2 cut(s) 1366, 1690
PmlI CACGTG 2 cut(s) 1366, 1690
Ppu21I YACGTR 3 cut(s) 1366, 1690, 1918
PpuMI RGGWCCY 1 cut(s) 678
PshAI GACNNNNGTC 1 cut(s) 1031
PshBI ATTAAT 1 cut(s) 1260
Psp5II RGGWCCY 1 cut(s) 678
Psp6I CCWGG 3 cut(s) 498, 1959, 2257
PspCI CACGTG 2 cut(s) 1366, 1690
PspFI CCCAGC 2 cut(s) 718, 1444
PspGI CCWGG 3 cut(s) 498, 1959, 2257
PspLI CGTACG 1 cut(s) 933
PspOMI GGGCCC 2 cut(s) 502, 1613
PspPPI RGGWCCY 1 cut(s) 678
PstI CTGCAG 1 cut(s) 2155
PsuI RGATCY 2 cut(s) 1479, 1751
PsyI GACNNNGTC 3 cut(s) 311, 437, 1901
RruI TCGCGA 1 cut(s) 1322
RsaI GTAC 7 cut(s) 176, 403, 647, 710, 935, 1148, 2113
RsaNI GTAC 7 cut(s) 175, 402, 646, 709, 934, 1147, 2112
RseI CAYNNNNRTG 4 cut(s) 1010, 1382, 1961, 2270
Rsr2I CGGWCCG 1 cut(s) 2094
RsrII CGGWCCG 1 cut(s) 2094
SacII CCGCGG 1 cut(s) 1525
SaqAI TTAA 6 cut(s) 96, 1260, 1653, 2024, 2142, 2213
Sau3AI GATC 2 cut(s) 1479, 1751
SduI GDGCHC 4 cut(s) 506, 999, 1617, 1739
SfaNI GCATC 4 cut(s) 471, 1119, 1436, 1732
SfcI CTRYAG 3 cut(s) 1449, 2151, 2286
Sfr303I CCGCGG 1 cut(s) 1525
SgrBI CCGCGG 1 cut(s) 1525
SinI GGWCC 5 cut(s) 678, 986, 1486, 1879, 2094
SmaI CCCGGG 2 cut(s) 1101, 1884
SmiMI CAYNNNNRTG 4 cut(s) 1010, 1382, 1961, 2270
SpeI ACTAGT 1 cut(s) 1348
SphI GCATGC 1 cut(s) 2015
Sse9I AATT 8 cut(s) 1257, 1261, 1458, 1933, 1939, 1984, 2026, 2119
SspMI CTAG 6 cut(s) 1205, 1349, 1971, 2268, 2319, 2353
StyI CCWWGG 5 cut(s) 253, 741, 768, 2238, 2325
TaiI ACGT 7 cut(s) 315, 714, 870, 1287, 1368, 1692, 1920
TaqI TCGA 6 cut(s) 453, 609, 840, 912, 1050, 1335
TaqII GACCGA 1 cut(s) 507
TasI AATT 8 cut(s) 1257, 1261, 1458, 1933, 1939, 1984, 2026, 2119
TfiI GAWTC 1 cut(s) 1531
Tru1I TTAA 6 cut(s) 96, 1260, 1653, 2024, 2142, 2213
Tru9I TTAA 6 cut(s) 96, 1260, 1653, 2024, 2142, 2213
TscAI CASTG 7 cut(s) 220, 536, 691, 1006, 2175, 2250, 2317
TseI GCWGC 6 cut(s) 104, 698, 810, 861, 1388, 2150
TspDTI ATGAA 2 cut(s) 106, 644
TspGWI ACGGA 3 cut(s) 636, 951, 1661
TspMI CCCGGG 2 cut(s) 1099, 1882
TspRI CASTG 7 cut(s) 220, 536, 691, 1006, 2175, 2250, 2317
Tth111I GACNNNGTC 3 cut(s) 311, 437, 1901
VpaK11BI GGWCC 5 cut(s) 678, 986, 1486, 1879, 2094
VspI ATTAAT 1 cut(s) 1260
XapI RAATTY 1 cut(s) 2119
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 1 cut(s) 2015
XmaI CCCGGG 2 cut(s) 1099, 1882
XmiI GTMKAC 1 cut(s) 2078
XmnI GAANNNNTTC 1 cut(s) 1261
XspI CTAG 6 cut(s) 1205, 1349, 1971, 2268, 2319, 2353
ZraI GACGTC 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.