Rorug02G0400700

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017066
Physical Location & Seq
Forward (+)
51123479 .. 51123883
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0400700.1

Sequence Viewer

Length: 405 bp
ATGCGGGCAAGTCTCTTGCTTATTATTCATCAAGGTATGGTTAGTGTTGACATAGAGACGGATTGTGCCATGGTGATTTCTCCTCATACAAGTAATACTGAGGACCTTTCAGAGGTTGGCTGCATTGTTGAGGATTGCAAAGCGTATATGCGTGCAATTCCATCTCTTAATCTTTACTCTGTCTGTAGAGAAGAAAATTGTGTAGCCCATAGATTGGCGCACCTTGCTAGAGAAAATTACTTAGATGATTATTGGCTAGATGAGGCTCCTGTGATCATCTGGGATGCACTCTATGAGAATTCTTGTACTAGTACTCGAGGTGTAGGTAAAATGTCCCCCTCACTGTACAATAATCTTTCAATTATGAATAATATTTCCAGGCTTGGGGCTGAGCCTCCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

134

Amino Acids

14.95

Weight (kDa)

4.69

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 5 - 75 1.7e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 214
AciI CCGC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 298
AfaI GTAC 3 cut(s) 307, 313, 347
AfiI CCNNNNNNNGG 3 cut(s) 112, 214, 384
AgsI TTSAA 1 cut(s) 360
AhlI ACTAGT 1 cut(s) 308
AjnI CCWGG 1 cut(s) 377
Alw26I GTCTC 2 cut(s) 17, 50
Ama87I CYCGRG 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 315
AvaII GGWCC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 379
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 17, 50
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 3 cut(s) 228, 257, 309
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 1 cut(s) 121
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 122
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 379
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 274
BmuI ACTGGG 1 cut(s) 393
Bpu1102I GCTNAGC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 69
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 214, 384
Bse1I ACTGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 3 cut(s) 112, 214, 384
BseMII CTCAG 2 cut(s) 90, 381
BseNI ACTGG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 315
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 3 cut(s) 112, 214, 384
BsmAI GTCTC 2 cut(s) 17, 50
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 1 cut(s) 273
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 1 cut(s) 4
BspCNI CTCAG 2 cut(s) 91, 382
BspLI GGNNCC 1 cut(s) 267
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 345
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 6, 153
BstDEI CTNAG 3 cut(s) 99, 241, 390
BstDSI CCRYGG 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 1 cut(s) 276
BstMAI GTCTC 2 cut(s) 17, 50
BstMBI GATC 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 107
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 2 cut(s) 6, 153
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 3 cut(s) 306, 312, 346
CviAII CATG 1 cut(s) 70
CviJI RGCY 7 cut(s) 120, 206, 256, 266, 382, 389, 394
CviKI_1 RGCY 7 cut(s) 120, 206, 256, 266, 382, 389, 394
CviQI GTAC 3 cut(s) 306, 312, 346
DdeI CTNAG 3 cut(s) 99, 241, 390
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 1 cut(s) 73
FaiI YATR 9 cut(s) 38, 53, 71, 87, 147, 149, 210, 294, 365
FaqI GGGAC 1 cut(s) 319
FatI CATG 1 cut(s) 69
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 3 cut(s) 228, 257, 309
GlaI GCGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 73
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4V TGCA 4 cut(s) 123, 138, 155, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 3 cut(s) 99, 241, 390
Hsp92II CATG 1 cut(s) 73
HspAI GCGC 1 cut(s) 218
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 4 cut(s) 265, 282, 364, 391
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 274
MaeI CTAG 3 cut(s) 228, 257, 309
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 1 cut(s) 203
MluCI AATT 5 cut(s) 156, 196, 235, 298, 360
MnlI CCTC 8 cut(s) 93, 94, 106, 124, 256, 311, 349, 405
MseI TTAA 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 224
NcoI CCATGG 1 cut(s) 69
NdeII GATC 1 cut(s) 273
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 103
PspPPI RGGWCCY 1 cut(s) 103
PspXI VCTCGAGB 1 cut(s) 315
RsaI GTAC 3 cut(s) 307, 313, 347
RsaNI GTAC 3 cut(s) 306, 312, 346
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 1 cut(s) 273
Sau96I GGNCC 1 cut(s) 103
ScaI AGTACT 1 cut(s) 313
ScrFI CCNGG 1 cut(s) 379
SetI ASST 6 cut(s) 37, 108, 117, 225, 322, 328
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 315
SinI GGWCC 1 cut(s) 103
SlaI CTCGAG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
SpeI ACTAGT 1 cut(s) 308
Sse9I AATT 5 cut(s) 156, 196, 235, 298, 360
SsiI CCGC 1 cut(s) 4
SspI AATATT 1 cut(s) 373
SspMI CTAG 3 cut(s) 228, 257, 309
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 345
TaqI TCGA 1 cut(s) 316
TasI AATT 5 cut(s) 156, 196, 235, 298, 360
TatI WGTACW 3 cut(s) 305, 311, 345
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 348
TseI GCWGC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 17, 380
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 1 cut(s) 348
Van91I CCANNNNNTGG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 103
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 315
XspI CTAG 3 cut(s) 228, 257, 309
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.