Rh2CG445500

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017066
Physical Location & Seq
Reverse (-)
60336269 .. 60339962
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG445500.1

Sequence Viewer

Length: 2322 bp
ATGTCTCTCTCTATCTCCATCCCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTAGCTTCAGCTTCTTCAGATTCCAAGACCTTCATAGTTCAAGTCCAACCTGATTCCAAGCCCTCAGTTTTCCCAACCCATAAACACTGGTATGAGTCTTCACTGTCCTCATTATCTTCTGAACCCACACCGTTGATACATACTTACAACACAGTCTTCCATGGCTTCTCTGCCAAGCTCTCTCCTTCCCAAGCCCAAAAGCTCCAGTCTTTGCCTCATGTTTTGGCTCTCATTCCCGAGCAAGTGCGCCGCCTCCACACCACTCGCTCGCCGGAGTTCCTCGGCCTCCGGTCCACTGACACTGCTGGGCTTCTCAAAGAATCGGACTTTGGTTCGGATCTCGTGATCGGAGTTATTGATACCGGAGTTTGGCCCGAGAGGCAGAGCTTCAACGACCACGACCTTAGTCCGGTTCCGTCCAAATGGAAGGGCCAGTGCGTCGCCGGAGAGAACTTTCTGGCTAGCTCGTGTAACCGCAAGCTCATCGGAGCGAGGTACTTCTGCGGCGGGTACGAGTCCACCAACGGCAAGATGAACCAGACTACGGAGTTTCGATCTCCCAGAGACACTGACGGCCACGGCACTCACACCGCCTCGATCGCCGCCGGCCGTTACGTCTTTCCGGCGTCTACTCTCGGCTATGCTAAAGGCGTCGCCGCCGGCATGGCTCCCAAGGCCCGGCTAGCAGCCTACAAGGTCTGCTGGAACGCCGGCTGTTACGACTCTGACATTCTCGCCGCCTTCGACTCCGCCGTCGCCGACGGATGCGACGTCGTCTCCCTCAGCGTCGGCGGGGTCGTTGTCCCCTACTACCTTGACGCCATTGCCATCGGAGCCTTTGGAGCCTCAGACGCCGGAGTATTTGTCTCGGCCTCCGCCGGAAATGGCGGCCCCGGTGGGCTCACGGTGACCAATGTGGCTCCATGGGTCACCACCGTCGGCGCCGGAACAATCGACCGTGACTTCCCTGCTGATGTTAAGCTCGGCAACGGCAGAATCATCCCCGGCATGAGTGTTTATGGCGGGCCGGGTCTCCCCCCGGGTCGGATGTTCCCGTTAGTTTACGCCGGCAGCGAAGGCGGTGATGGGTACTCCTCTTCTCTATGCCTAGAAGGTTCTCTTGACCAAAGGCTGGTGAAGAACAAGATAGTTGTTTGCGACAGAGGAATCAATTCAAGAGCTGCAAAAGGTGAGGTGGTGAAGAAAGCCGGAGGAGTTGGCATGATTCTTGCAAATGGTGTCTTCGACGGTGAAGGACTAGTAGCCGACTGCCATGTCTTACCTGCCACCGCCGTCGCTGCTTCCACCGGTGACGAAATAAGGCAGTACATAACAGCTGCGTCCAAGTCCAAATCACCACCAACAGCTACCATTTTGTTCAAGGGTACTCGGATCCGTGTCAAACCAGCACCGGTAGTTGCCTCATTTTCGGCCCGTGGCCCCAACCCGGAAGCGCCGGAAATACTGAAGCCGGATGTGATAGCACCTGGACTGAACATACTTGCAGCTTGGCCGGATAAAGTGGGGCCATCAGGCATTCCTTCGGATAAGAGAACCACCGAGTTCAACATTCTTTCGGGTACGTCAATGGCTTGCCCACACGTTTCGGGTCTGGCCGCCTTGTTGAAGGCGGCCCACCCGGAATGGAGTCCGGCTGCTATTAGATCGGCTCTAATGACGACAGCCTACACCGTCGACAATAGAGGCGAGACGATGCTTGACGAGTCTAGTGGGAATACTTCGACAGTTATGGACTTTGGGGCTGGCCATGTCCATCCGCAAAAGGCAATGGACCCTGGATTGGTTTACGACATAAGTTCATCCGATTATGTCGATTTCTTGTGTAATTCCAATTACACGACTAAGAACATCCAGGTGATCACTAGGAAAGTAGCAAATTGCAATGGGGCAAAGAGGGCAGGCCATTCGGGGAATCTGAATTACCCATCATTGTCTGTGGTTTTCCAGCAATACGGAAAGCGAAAGATGTCTACGCATTTTATTCGGACGGTCACAAATGTCGGGGACCCGAAATCGGTTTATCAGGTGACGATCAGGCCACCCCGCGGAATGTCGGTGACAGTGAAGCCGGAGAAGCTTGCGTTTAGGAGGGTGGGGCAGAAATTGAGCTTCCTTGTGAGGGTTCAAGCTAGGGAAGTCAAGCTTTCTCCTGGAAGTAGTAGCATGAAGAGTGGGTCTATACTGTGGTCAGATGGGAAACACACTGTGACTAGTCCTTTGGTTGTGACAATGCAACAACCTCTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

773

Amino Acids

81.98

Weight (kDa)

9.07

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 27 - 103 5.4e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 128 - 595 9.9e-49 Subtilase family
PA PF02225 369 - 457 2e-11 PA domain
fn3_6 PF17766 662 - 766 5.3e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 803, 1325
AatII GACGTC 1 cut(s) 825
Acc36I ACCTGC 1 cut(s) 1342
AccB1I GGYRCC 1 cut(s) 992
AccB7I CCANNNNNTGG 1 cut(s) 1183
AccI GTMKAC 3 cut(s) 680, 1746, 2042
AccII CGCG 1 cut(s) 2118
AclWI GGATC 3 cut(s) 396, 1438, 1451
AcoI YGGCCR 5 cut(s) 625, 658, 1562, 1665, 1816
AcuI CTGAAG 3 cut(s) 42, 51, 1538
AcyI GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
AdeI CACNNNGTG 1 cut(s) 2278
AfaI GTAC 6 cut(s) 548, 563, 1142, 1379, 1438, 1633
AflIII ACRYGT 1 cut(s) 1651
AgeI ACCGGT 2 cut(s) 1358, 1462
AgsI TTSAA 7 cut(s) 92, 442, 1227, 1432, 1618, 1678, 2198
AhlI ACTAGT 2 cut(s) 1309, 2282
AjnI CCWGG 4 cut(s) 1537, 1846, 1923, 2221
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
Alw26I GTCTC 6 cut(s) 9, 609, 832, 922, 1088, 1754
AlwI GGATC 3 cut(s) 396, 1438, 1451
Ama87I CYCGRG 3 cut(s) 287, 425, 1090
ApeKI GCWGC 7 cut(s) 737, 1122, 1232, 1349, 1388, 1556, 1706
ArsI GACNNNNNNTTYG 2 cut(s) 2272, 2304
AsiGI ACCGGT 2 cut(s) 1358, 1462
Asp700I GAANNNNTTC 1 cut(s) 1222
AspLEI GCGC 3 cut(s) 300, 995, 1507
AsuC2I CCSGG 8 cut(s) 730, 945, 1056, 1080, 1091, 1092, 1499, 1691
AsuNHI GCTAGC 2 cut(s) 512, 733
AvaI CYCGRG 3 cut(s) 287, 425, 1090
AvaII GGWCC 3 cut(s) 342, 1843, 2077
BaeI ACNNNNGTAYC 2 cut(s) 402, 435
BalI TGGCCA 1 cut(s) 1818
BamHI GGATCC 1 cut(s) 1443
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 954
BauI CACGAG 2 cut(s) 392, 517
BbsI GAAGAC 3 cut(s) 141, 199, 1285
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 7 cut(s) 749, 1134, 1219, 1336, 1375, 1568, 1693
BccI CCATC 7 cut(s) 26, 887, 1130, 1588, 1833, 2005, 2258
BceAI ACGGC 7 cut(s) 592, 640, 645, 646, 788, 1057, 1328
BcgI CGANNNNNNTGC 6 cut(s) 517, 551, 1959, 1993, 2036, 2070
BciT130I CCWGG 4 cut(s) 1539, 1848, 1925, 2223
BclI TGATCA 1 cut(s) 1929
BcnI CCSGG 8 cut(s) 730, 945, 1056, 1080, 1091, 1092, 1499, 1691
BcoDI GTCTC 6 cut(s) 9, 609, 832, 922, 1088, 1754
BcuI ACTAGT 2 cut(s) 1309, 2282
BfaI CTAG 9 cut(s) 513, 734, 1160, 1310, 1779, 1935, 2202, 2283, 2315
BfoI RGCGCY 2 cut(s) 996, 1508
BfuAI ACCTGC 1 cut(s) 1342
BglI GCCNNNNNGGC 2 cut(s) 430, 716
Bme18I GGWCC 3 cut(s) 342, 1843, 2077
BmeT110I CYCGRG 3 cut(s) 287, 425, 1090
BmsI GCATC 2 cut(s) 806, 1755
BmtI GCTAGC 2 cut(s) 516, 737
BoxI GACNNNNGTC 1 cut(s) 456
BpiI GAAGAC 3 cut(s) 141, 199, 1285
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 833
BpuMI CCSGG 8 cut(s) 730, 945, 1056, 1080, 1091, 1092, 1499, 1691
BsaHI GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
BsaI GGTCTC 1 cut(s) 1088
BsaWI WCCGGW 5 cut(s) 339, 413, 460, 1358, 1462
BsaXI ACNNNNNCTCC 4 cut(s) 812, 842, 900, 930
Bse118I RCCGGY 6 cut(s) 656, 710, 761, 1118, 1358, 1462
Bse1I ACTGG 3 cut(s) 143, 256, 484
Bse3DI GCAATG 3 cut(s) 873, 1845, 1960
BseBI CCWGG 4 cut(s) 1539, 1848, 1925, 2223
BseGI GGATG 8 cut(s) 18, 821, 1050, 1104, 1531, 1825, 1871, 1920
BseMI GCAATG 3 cut(s) 873, 1845, 1960
BseMII CTCAG 3 cut(s) 129, 847, 912
BseNI ACTGG 3 cut(s) 143, 256, 484
BseRI GAGGAG 2 cut(s) 1135, 1278
BseX3I CGGCCG 1 cut(s) 658
BseXI GCAGC 7 cut(s) 749, 1134, 1219, 1336, 1375, 1568, 1693
BseYI CCCAGC 1 cut(s) 356
Bsh1236I CGCG 1 cut(s) 2118
Bsh1285I CGRYCG 3 cut(s) 651, 661, 1009
BshNI GGYRCC 1 cut(s) 992
BshTI ACCGGT 2 cut(s) 1358, 1462
BsiEI CGRYCG 3 cut(s) 651, 661, 1009
BsiHKCI CYCGRG 3 cut(s) 287, 425, 1090
BslFI GGGAC 2 cut(s) 839, 2090
BsmAI GTCTC 6 cut(s) 9, 609, 832, 922, 1088, 1754
BsmBI CGTCTC 2 cut(s) 832, 1754
BsmFI GGGAC 2 cut(s) 839, 2090
BsmI GAATGC 1 cut(s) 1587
Bso31I GGTCTC 1 cut(s) 1088
BsoBI CYCGRG 3 cut(s) 287, 425, 1090
Bsp1286I GDGCHC 1 cut(s) 954
Bsp143I GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
Bsp19I CCATGG 2 cut(s) 211, 974
BspCNI CTCAG 3 cut(s) 128, 846, 911
BspFNI CGCG 1 cut(s) 2118
BspMI ACCTGC 1 cut(s) 1342
BspOI GCTAGC 2 cut(s) 516, 737
BspPI GGATC 3 cut(s) 396, 1438, 1451
BspT107I GGYRCC 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 1088
BsrDI GCAATG 3 cut(s) 873, 1845, 1960
BsrFI RCCGGY 6 cut(s) 656, 710, 761, 1118, 1358, 1462
BsrI ACTGG 3 cut(s) 143, 256, 484
BssAI RCCGGY 6 cut(s) 656, 710, 761, 1118, 1358, 1462
BssMI GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
BssNI GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
BssSI CACGAG 2 cut(s) 392, 517
BssT1I CCWWGG 3 cut(s) 211, 723, 974
Bst2BI CACGAG 2 cut(s) 392, 517
Bst2UI CCWGG 4 cut(s) 1539, 1848, 1925, 2223
Bst6I CTCTTC 2 cut(s) 1153, 2234
BstACI GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
BstDEI CTNAG 6 cut(s) 52, 115, 455, 833, 898, 1914
BstDSI CCRYGG 5 cut(s) 211, 628, 974, 1486, 2116
BstEII GGTNACC 2 cut(s) 958, 979
BstF5I GGATG 8 cut(s) 18, 821, 1050, 1104, 1531, 1825, 1871, 1920
BstFNI CGCG 1 cut(s) 2118
BstH2I RGCGCY 2 cut(s) 996, 1508
BstHHI GCGC 3 cut(s) 300, 995, 1507
BstKTI GATC 8 cut(s) 391, 399, 608, 651, 1446, 1718, 1932, 2106
BstMAI GTCTC 6 cut(s) 9, 609, 832, 922, 1088, 1754
BstMBI GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
BstMCI CGRYCG 3 cut(s) 651, 661, 1009
BstNI CCWGG 4 cut(s) 1539, 1848, 1925, 2223
BstPAI GACNNNNGTC 1 cut(s) 456
BstPI GGTNACC 2 cut(s) 958, 979
BstUI CGCG 1 cut(s) 2118
BstV1I GCAGC 7 cut(s) 749, 1134, 1219, 1336, 1375, 1568, 1693
BstV2I GAAGAC 3 cut(s) 141, 199, 1285
BstX2I RGATCY 2 cut(s) 388, 1443
BstYI RGATCY 2 cut(s) 388, 1443
BstZI CGGCCG 1 cut(s) 658
BtgI CCRYGG 5 cut(s) 211, 628, 974, 1486, 2116
BtsCI GGATG 8 cut(s) 18, 821, 1050, 1104, 1531, 1825, 1871, 1920
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 8 cut(s) 136, 152, 345, 351, 491, 618, 2139, 2274
BveI ACCTGC 1 cut(s) 1342
CfoI GCGC 3 cut(s) 300, 995, 1507
Cfr10I RCCGGY 6 cut(s) 656, 710, 761, 1118, 1358, 1462
Cfr42I CCGCGG 1 cut(s) 2119
Cfr9I CCCGGG 1 cut(s) 1090
CseI GACGC 7 cut(s) 478, 666, 691, 826, 878, 911, 1380
Csp6I GTAC 6 cut(s) 547, 562, 1141, 1378, 1437, 1632
CspAI ACCGGT 2 cut(s) 1358, 1462
CviAII CATG 9 cut(s) 212, 269, 715, 975, 1060, 1273, 1325, 1820, 2236
CviQI GTAC 6 cut(s) 547, 562, 1141, 1378, 1437, 1632
DdeI CTNAG 6 cut(s) 52, 115, 455, 833, 898, 1914
DinI GGCGCC 1 cut(s) 994
DpnI GATC 8 cut(s) 390, 398, 607, 650, 1445, 1717, 1931, 2105
DpnII GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
DraIII CACNNNGTG 1 cut(s) 2278
DrdI GACNNNNNNGTC 2 cut(s) 803, 1325
DseDI GACNNNNNNGTC 2 cut(s) 803, 1325
EaeI YGGCCR 5 cut(s) 625, 658, 1562, 1665, 1816
EagI CGGCCG 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 1153, 2234
EarI CTCTTC 2 cut(s) 1153, 2234
EciI GGCGGA 2 cut(s) 790, 916
EclXI CGGCCG 1 cut(s) 658
Eco130I CCWWGG 3 cut(s) 211, 723, 974
Eco24I GRGCYC 1 cut(s) 954
Eco31I GGTCTC 1 cut(s) 1088
Eco47I GGWCC 3 cut(s) 342, 1843, 2077
Eco52I CGGCCG 1 cut(s) 658
Eco57I CTGAAG 3 cut(s) 42, 51, 1538
Eco88I CYCGRG 3 cut(s) 287, 425, 1090
Eco91I GGTNACC 2 cut(s) 958, 979
EcoO109I RGGNCCY 1 cut(s) 2077
EcoO65I GGTNACC 2 cut(s) 958, 979
EcoRII CCWGG 4 cut(s) 1537, 1846, 1923, 2221
EcoT14I CCWWGG 3 cut(s) 211, 723, 974
EcoT38I GRGCYC 1 cut(s) 954
EgeI GGCGCC 1 cut(s) 994
EheI GGCGCC 1 cut(s) 994
ErhI CCWWGG 3 cut(s) 211, 723, 974
Esp3I CGTCTC 2 cut(s) 832, 1754
FaeI CATG 9 cut(s) 215, 272, 718, 978, 1063, 1276, 1328, 1823, 2239
FalI AAGNNNNNCTT 4 cut(s) 1155, 1187, 2199, 2231
FaqI GGGAC 2 cut(s) 839, 2090
FatI CATG 9 cut(s) 211, 268, 714, 974, 1059, 1272, 1324, 1819, 2235
FauI CCCGC 4 cut(s) 551, 836, 1067, 2123
FbaI TGATCA 1 cut(s) 1929
FblI GTMKAC 3 cut(s) 680, 1746, 2042
FokI GGATG 8 cut(s) 5, 828, 1037, 1111, 1538, 1812, 1858, 1907
FriOI GRGCYC 1 cut(s) 954
FspBI CTAG 9 cut(s) 513, 734, 1160, 1310, 1779, 1935, 2202, 2283, 2315
GlaI GCGC 3 cut(s) 299, 994, 1506
GsaI CCCAGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 2 cut(s) 996, 1508
HgaI GACGC 7 cut(s) 478, 666, 691, 826, 878, 911, 1380
HhaI GCGC 3 cut(s) 300, 995, 1507
Hin1I GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
Hin1II CATG 9 cut(s) 215, 272, 718, 978, 1063, 1276, 1328, 1823, 2239
Hin6I GCGC 3 cut(s) 298, 993, 1505
HinP1I GCGC 3 cut(s) 298, 993, 1505
HincII GTYRAC 1 cut(s) 1747
HindII GTYRAC 1 cut(s) 1747
HindIII AAGCTT 2 cut(s) 2147, 2213
Hpy166II GTNNAC 7 cut(s) 345, 570, 681, 1114, 1747, 1858, 2043
Hpy188III TCNNGA 4 cut(s) 287, 394, 1172, 1227
Hpy8I GTNNAC 7 cut(s) 345, 570, 681, 1114, 1747, 1858, 2043
HpyAV CCTTC 9 cut(s) 91, 246, 472, 802, 1121, 1157, 1298, 1602, 1672
HpyCH4IV ACGT 4 cut(s) 666, 822, 1634, 1653
HpyCH4V TGCA 5 cut(s) 1235, 1283, 1556, 1953, 2305
HpyF3I CTNAG 6 cut(s) 52, 115, 455, 833, 898, 1914
HpySE526I ACGT 4 cut(s) 666, 822, 1634, 1653
Hsp92I GRCGYC 6 cut(s) 677, 702, 822, 870, 903, 993
Hsp92II CATG 9 cut(s) 215, 272, 718, 978, 1063, 1276, 1328, 1823, 2239
HspAI GCGC 3 cut(s) 298, 993, 1505
KasI GGCGCC 1 cut(s) 992
KflI GGGWCCC 1 cut(s) 2077
KroI GCCGGC 4 cut(s) 656, 710, 761, 1118
KroNI GCCGGC 4 cut(s) 658, 712, 763, 1120
Ksp22I TGATCA 1 cut(s) 1929
KspI CCGCGG 1 cut(s) 2119
Kzo9I GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
LmnI GCTCC 6 cut(s) 258, 539, 724, 884, 893, 976
Lsp1109I GCAGC 7 cut(s) 749, 1134, 1219, 1336, 1375, 1568, 1693
LweI GCATC 2 cut(s) 806, 1755
MaeI CTAG 9 cut(s) 513, 734, 1160, 1310, 1779, 1935, 2202, 2283, 2315
MaeII ACGT 4 cut(s) 666, 822, 1634, 1653
MalI GATC 8 cut(s) 390, 398, 607, 650, 1445, 1717, 1931, 2105
MboI GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
MflI RGATCY 2 cut(s) 388, 1443
MhlI GDGCHC 1 cut(s) 954
MlsI TGGCCA 1 cut(s) 1818
MluCI AATT 6 cut(s) 1222, 1897, 1903, 1948, 1990, 2174
MluNI TGGCCA 1 cut(s) 1818
Mly113I GGCGCC 1 cut(s) 993
MlyI GAGTC 6 cut(s) 155, 575, 767, 791, 1708, 1784
MmeI TCCRAC 2 cut(s) 121, 1076
Mox20I TGGCCA 1 cut(s) 1818
MroNI GCCGGC 4 cut(s) 656, 710, 761, 1118
MroXI GAANNNNTTC 1 cut(s) 1222
MscI TGGCCA 1 cut(s) 1818
MseI TTAA 1 cut(s) 1029
MslI CAYNNNNRTG 2 cut(s) 141, 1925
Msp20I TGGCCA 1 cut(s) 1818
MspA1I CMGCKG 2 cut(s) 1388, 2118
Mva1269I GAATGC 1 cut(s) 1587
MvaI CCWGG 4 cut(s) 1539, 1848, 1925, 2223
MvnI CGCG 1 cut(s) 2118
NaeI GCCGGC 4 cut(s) 658, 712, 763, 1120
NarI GGCGCC 1 cut(s) 993
NciI CCSGG 8 cut(s) 730, 945, 1056, 1080, 1091, 1092, 1499, 1691
NcoI CCATGG 2 cut(s) 211, 974
NdeII GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
NgoMIV GCCGGC 4 cut(s) 656, 710, 761, 1118
NheI GCTAGC 2 cut(s) 512, 733
NlaIII CATG 9 cut(s) 215, 272, 718, 978, 1063, 1276, 1328, 1823, 2239
NmeAIII GCCGAG 4 cut(s) 312, 666, 899, 1014
NmuCI GTSAC 9 cut(s) 958, 979, 1010, 1361, 2062, 2098, 2128, 2278, 2296
PcsI WCGNNNNNNNCGW 5 cut(s) 792, 801, 819, 846, 1122
PctI GAATGC 1 cut(s) 1587
PdiI GCCGGC 4 cut(s) 658, 712, 763, 1120
PdmI GAANNNNTTC 1 cut(s) 1222
PfeI GAWTC 7 cut(s) 71, 104, 371, 1047, 1218, 1276, 1984
PflFI GACNNNGTC 1 cut(s) 824
PflMI CCANNNNNTGG 1 cut(s) 1183
PfoI TCCNGGA 1 cut(s) 2221
PinAI ACCGGT 2 cut(s) 1358, 1462
Ple19I CGATCG 1 cut(s) 651
PleI GAGTC 6 cut(s) 154, 574, 767, 791, 1707, 1783
PluTI GGCGCC 1 cut(s) 996
PpsI GAGTC 6 cut(s) 154, 574, 767, 791, 1707, 1783
PpuMI RGGWCCY 1 cut(s) 2077
PshAI GACNNNNGTC 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 2077
Psp6I CCWGG 4 cut(s) 1537, 1846, 1923, 2221
PspEI GGTNACC 2 cut(s) 958, 979
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 4 cut(s) 1537, 1846, 1923, 2221
PspPPI RGGWCCY 1 cut(s) 2077
PsuI RGATCY 2 cut(s) 388, 1443
PsyI GACNNNGTC 1 cut(s) 824
PvuI CGATCG 1 cut(s) 651
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 6 cut(s) 548, 563, 1142, 1379, 1438, 1633
RsaNI GTAC 6 cut(s) 547, 562, 1141, 1378, 1437, 1632
RseI CAYNNNNRTG 2 cut(s) 141, 1925
SacII CCGCGG 1 cut(s) 2119
SalI GTCGAC 1 cut(s) 1745
SaqAI TTAA 1 cut(s) 1029
Sau3AI GATC 8 cut(s) 388, 396, 605, 648, 1443, 1715, 1929, 2103
SchI GAGTC 6 cut(s) 155, 575, 767, 791, 1708, 1784
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 2 cut(s) 806, 1755
SfoI GGCGCC 1 cut(s) 994
Sfr303I CCGCGG 1 cut(s) 2119
SgrAI CRCCGGYG 1 cut(s) 1358
SgrBI CCGCGG 1 cut(s) 2119
SinI GGWCC 3 cut(s) 342, 1843, 2077
SmaI CCCGGG 1 cut(s) 1092
SmiMI CAYNNNNRTG 2 cut(s) 141, 1925
SpeI ACTAGT 2 cut(s) 1309, 2282
Sse9I AATT 6 cut(s) 1222, 1897, 1903, 1948, 1990, 2174
SspDI GGCGCC 1 cut(s) 992
SspMI CTAG 9 cut(s) 513, 734, 1160, 1310, 1779, 1935, 2202, 2283, 2315
StyI CCWWGG 3 cut(s) 211, 723, 974
TaiI ACGT 4 cut(s) 669, 825, 1637, 1656
TaqI TCGA 8 cut(s) 604, 647, 795, 1005, 1296, 1746, 1793, 1884
TasI AATT 6 cut(s) 1222, 1897, 1903, 1948, 1990, 2174
TatI WGTACW 1 cut(s) 1377
TauI GCSGC 8 cut(s) 303, 558, 656, 710, 791, 942, 1670, 1685
TfiI GAWTC 7 cut(s) 71, 104, 371, 1047, 1218, 1276, 1984
Tru1I TTAA 1 cut(s) 1029
Tru9I TTAA 1 cut(s) 1029
TscAI CASTG 8 cut(s) 143, 159, 352, 358, 491, 625, 2139, 2281
TseFI GTSAC 9 cut(s) 958, 979, 1010, 1361, 2062, 2098, 2128, 2278, 2296
TseI GCWGC 7 cut(s) 737, 1122, 1232, 1349, 1388, 1556, 1706
Tsp45I GTSAC 9 cut(s) 958, 979, 1010, 1361, 2062, 2098, 2128, 2278, 2296
TspDTI ATGAA 4 cut(s) 73, 599, 1860, 2252
TspGWI ACGGA 5 cut(s) 456, 611, 828, 1436, 2040
TspMI CCCGGG 1 cut(s) 1090
TspRI CASTG 8 cut(s) 143, 159, 352, 358, 491, 625, 2139, 2281
Tth111I GACNNNGTC 1 cut(s) 824
Van91I CCANNNNNTGG 1 cut(s) 1183
VpaK11BI GGWCC 3 cut(s) 342, 1843, 2077
XmaI CCCGGG 1 cut(s) 1090
XmiI GTMKAC 3 cut(s) 680, 1746, 2042
XmnI GAANNNNTTC 1 cut(s) 1222
XspI CTAG 9 cut(s) 513, 734, 1160, 1310, 1779, 1935, 2202, 2283, 2315
ZraI GACGTC 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.