FvH4_6g35680

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017066
Physical Location & Seq
Reverse (-)
28166367 .. 28168998
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35680.t1

Sequence Viewer

Length: 1740 bp
ATGAACCAGACGACGGAGTTTCGATCTCCTAGAGACACTGACGGCCACGGCACCCACACCGCCTCCATCGCCGCCGGCCGTTACGTCTTTCCGGCGTCCACTCTCGGCTATGCTAAAGGCGTCGCCGCCGGCATGGCTCCCAAGGCGCGGCTAGCAGCCTACAAGGTCTGCTGGAACGCCGGCTGTTACGACTCCGACATTCTCGCCGCCTTTGACGCCGCCGTGGCCGACGGATGCGACGTCGTCTCCCTCAGCGTTGGCGGGGTCGTTGTTCCCTACTACCTTGACGCCATTGCTATTGGAGCCTTTGGTGCTTCAGACGCCGGAGTGTTCGTCTCGGCATCCGCCGGAAATGGCGGCCCCGGCGGGCTCACGGTGACCAATGTAGCCCCATGGGTGACAACCGTCGGCGCTGGAACGATCGATCGTGACTTCCCTGCTGATGTTAAGCTCGGAAATGGCAGAATCATCCCTGGCATGAGTGTTTATGGCGGGCCGGGTCTTCCCCCGGGTCGTATGTTCCCGTTAGTTTACGCCGGCAGCGAAGGTGGTGATGGGTACTCCTCTTCTCTATGCCTTGAAGGGTCTCTTGACAAAAGCCTGGTGAAAAACAAGATAGTTGTTTGCGACAGAGGAATTAATTCAAGAGCTGCAAAAGGTGAGGTGGTGAAGAAAGCCGGAGGAGTTGGCATGATTCTTGCAAATGGTGTTTTCGACGGTGAAGGACTAGTAGCCGACTGCCACGTCTTACCCGCCACCGCCGTCGCGGCCTCCACCGGTGACGAAATAAGGAAGTACATAACAGCTGCAGCTAAGTCCAAATCCCCACCAACAGCTACCATTTTGTTCAAGGGTACTCGAATCCGTGTCAAGCCGGCACCGGTAGTTGCCTCATTCTCGGCCCGTGGCCCCAACCCAGAAGCGCCCGAAATAGTAAAGCCGGATGTGATAGCACCAGGACTGAACATTCTTGCAGCCTGGCCGGATAAAGTAGGGCCGTCAGGTATTCCTTCCGATAAGAGGAGTACCGAGTTCAACATACTTTCGGGTACATCAATGGCTTGTCCACACGTTTCTGGTTTGGCGGCCTTGTTGAAGGCCGCCCACCCGGAATGGAGTCCGGCTGCCATTAGATCGGCTCTAATGACAACGGCCTACACTGTGGACAATAGAGGCGAGACAATGCTTGATGAGTCGAGCGGGAATACTTCAACAGTCATGGACTTCGGTGCCGGTCATGTCCATCCGCAAAAGGCAATGGACCCTGGATTGATTTACGACATAAGTTCATCCGATTATGTCGATTTCTTGTGCAATTCCAACTACACGACCAAGAACATTCAGGTGGTCACTAGGAAATTAGCAAATTGCAATGGGGCAAAGAGGGCAGGCCATTCGGGGAATCTGAACTATCCATCATTGTCTGTGGTGTTCCAGCAATATGGAAAGCGAAAGAAGTCTACACATTTTATTCGGACGGTGACAAATGTTGGGGACCCAAAGTCAGTTTATCATGTGACGATTAGGCCACCGAGGGGAATGTCAGTGACGGTGCAGCCAGAGAAGCTTGCGTTTAGGAGGGTTGGGCAGAAATTGAACTTCCTTGTGAGGGTTCAGGCTAGGGAAGTGAAGCTTTCACCCGGAAGTAGTAGCATGGAGAGTGGGTCTATAATGTGGTCAGATGGCAAACACACTGTGACTAGTCCTTTGGTTGTGACAATGCAACAACCTCTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

580

Amino Acids

60.39

Weight (kDa)

9.14

Isoelectric Point (pI)

31.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 4 - 401 3.1e-43 Subtilase family
PA PF02225 175 - 263 1.3e-11 PA domain
fn3_6 PF17766 468 - 572 1.7e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 743
AatII GACGTC 1 cut(s) 243
AccB1I GGYRCC 3 cut(s) 50, 877, 1229
AccBSI CCGCTC 1 cut(s) 1200
AccI GTMKAC 1 cut(s) 1460
AccII CGCG 2 cut(s) 148, 767
AcoI YGGCCR 4 cut(s) 43, 76, 225, 980
AcuI CTGAAG 1 cut(s) 300
AcyI GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
AdeI CACNNNGTG 1 cut(s) 1696
AfaI GTAC 5 cut(s) 560, 797, 856, 1027, 1051
AfiI CCNNNNNNNGG 5 cut(s) 13, 147, 1020, 1535, 1609
AflIII ACRYGT 1 cut(s) 1069
AgeI ACCGGT 2 cut(s) 776, 880
AgsI TTSAA 7 cut(s) 581, 645, 850, 1036, 1096, 1212, 1597
AhdI GACNNNNNGTC 1 cut(s) 1501
AhlI ACTAGT 2 cut(s) 727, 1700
AjiI CACGTC 1 cut(s) 745
AjnI CCWGG 5 cut(s) 472, 600, 955, 977, 1264
AluBI AGCT 7 cut(s) 451, 650, 806, 812, 836, 1567, 1633
AluI AGCT 7 cut(s) 451, 650, 806, 812, 836, 1567, 1633
Alw26I GTCTC 5 cut(s) 27, 250, 340, 591, 1172
Ama87I CYCGRG 1 cut(s) 508
ApeKI GCWGC 8 cut(s) 155, 540, 650, 806, 809, 974, 1124, 1555
ArsI GACNNNNNNTTYG 2 cut(s) 1690, 1722
AseI ATTAAT 1 cut(s) 639
AsiGI ACCGGT 2 cut(s) 776, 880
Asp700I GAANNNNTTC 1 cut(s) 640
AspLEI GCGC 3 cut(s) 148, 413, 925
AspS9I GGNCC 7 cut(s) 359, 494, 901, 908, 995, 1261, 1495
AsuC2I CCSGG 6 cut(s) 363, 498, 509, 510, 1109, 1641
AsuNHI GCTAGC 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 508
AvaII GGWCC 2 cut(s) 1261, 1495
BanI GGYRCC 3 cut(s) 50, 877, 1229
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 494
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 8 cut(s) 167, 552, 637, 793, 821, 986, 1111, 1567
BccI CCATC 5 cut(s) 74, 548, 1251, 1423, 1676
BceAI ACGGC 7 cut(s) 58, 63, 64, 206, 746, 982, 1167
BcgI CGANNNNNNTGC 2 cut(s) 1377, 1411
BciT130I CCWGG 5 cut(s) 474, 602, 957, 979, 1266
BcnI CCSGG 6 cut(s) 363, 498, 509, 510, 1109, 1641
BcoDI GTCTC 5 cut(s) 27, 250, 340, 591, 1172
BcuI ACTAGT 2 cut(s) 727, 1700
BfaI CTAG 7 cut(s) 30, 152, 728, 1353, 1620, 1701, 1733
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 2 cut(s) 414, 926
BglI GCCNNNNNGGC 3 cut(s) 134, 224, 767
Bme18I GGWCC 2 cut(s) 1261, 1495
BmeRI GACNNNNNGTC 1 cut(s) 1501
BmeT110I CYCGRG 1 cut(s) 508
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 7 cut(s) 359, 494, 901, 908, 995, 1261, 1495
BmsI GCATC 2 cut(s) 224, 350
BmtI GCTAGC 1 cut(s) 155
BoxI GACNNNNGTC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 251
BpuMI CCSGG 6 cut(s) 363, 498, 509, 510, 1109, 1641
Bsa29I ATCGAT 1 cut(s) 423
BsaHI GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
BsaI GGTCTC 1 cut(s) 591
BsaWI WCCGGW 2 cut(s) 776, 880
BsaXI ACNNNNNCTCC 8 cut(s) 47, 77, 230, 260, 318, 348, 1649, 1679
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 147, 1020, 1535, 1609
Bse118I RCCGGY 8 cut(s) 74, 128, 179, 536, 776, 874, 880, 1232
Bse3DI GCAATG 3 cut(s) 291, 1263, 1378
BseBI CCWGG 5 cut(s) 474, 602, 957, 979, 1266
BseCI ATCGAT 1 cut(s) 423
BseGI GGATG 6 cut(s) 239, 341, 468, 949, 1243, 1289
BseLI CCNNNNNNNGG 5 cut(s) 13, 147, 1020, 1535, 1609
BseMI GCAATG 3 cut(s) 291, 1263, 1378
BseMII CTCAG 1 cut(s) 265
BseRI GAGGAG 3 cut(s) 553, 696, 1036
BseX3I CGGCCG 1 cut(s) 76
BseXI GCAGC 8 cut(s) 167, 552, 637, 793, 821, 986, 1111, 1567
BsgI GTGCAG 1 cut(s) 1574
Bsh1236I CGCG 2 cut(s) 148, 767
Bsh1285I CGRYCG 3 cut(s) 79, 423, 427
BshNI GGYRCC 3 cut(s) 50, 877, 1229
BshTI ACCGGT 2 cut(s) 776, 880
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 3 cut(s) 79, 423, 427
BsiHKCI CYCGRG 1 cut(s) 508
BslFI GGGAC 1 cut(s) 1508
BslI CCNNNNNNNGG 5 cut(s) 13, 147, 1020, 1535, 1609
BsmAI GTCTC 5 cut(s) 27, 250, 340, 591, 1172
BsmBI CGTCTC 2 cut(s) 250, 340
BsmFI GGGAC 1 cut(s) 1508
Bso31I GGTCTC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 4 cut(s) 23, 420, 424, 1133
Bsp19I CCATGG 1 cut(s) 392
BspCNI CTCAG 1 cut(s) 264
BspDI ATCGAT 1 cut(s) 423
BspFNI CGCG 2 cut(s) 148, 767
BspMAI CTGCAG 1 cut(s) 811
BspOI GCTAGC 1 cut(s) 155
BspT107I GGYRCC 3 cut(s) 50, 877, 1229
BspTNI GGTCTC 1 cut(s) 591
BsrBI CCGCTC 1 cut(s) 1200
BsrDI GCAATG 3 cut(s) 291, 1263, 1378
BsrFI RCCGGY 8 cut(s) 74, 128, 179, 536, 776, 874, 880, 1232
BssAI RCCGGY 8 cut(s) 74, 128, 179, 536, 776, 874, 880, 1232
BssMI GATC 4 cut(s) 23, 420, 424, 1133
BssNI GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
BssT1I CCWWGG 2 cut(s) 141, 392
Bst2UI CCWGG 5 cut(s) 474, 602, 957, 979, 1266
Bst4CI ACNGT 8 cut(s) 376, 406, 719, 1162, 1216, 1480, 1552, 1696
Bst6I CTCTTC 1 cut(s) 571
BstACI GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
BstDEI CTNAG 2 cut(s) 251, 813
BstDSI CCRYGG 4 cut(s) 46, 222, 392, 904
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 6 cut(s) 239, 341, 468, 949, 1243, 1289
BstFNI CGCG 2 cut(s) 148, 767
BstH2I RGCGCY 2 cut(s) 414, 926
BstHHI GCGC 3 cut(s) 148, 413, 925
BstKTI GATC 4 cut(s) 26, 423, 427, 1136
BstMAI GTCTC 5 cut(s) 27, 250, 340, 591, 1172
BstMBI GATC 4 cut(s) 23, 420, 424, 1133
BstMCI CGRYCG 3 cut(s) 79, 423, 427
BstNI CCWGG 5 cut(s) 474, 602, 957, 979, 1266
BstPAI GACNNNNGTC 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 376
BstSFI CTRYAG 1 cut(s) 807
BstUI CGCG 2 cut(s) 148, 767
BstV1I GCAGC 8 cut(s) 167, 552, 637, 793, 821, 986, 1111, 1567
BstV2I GAAGAC 1 cut(s) 494
BstXI CCANNNNNNTGG 1 cut(s) 1442
BstZI CGGCCG 1 cut(s) 76
Bsu15I ATCGAT 1 cut(s) 423
BsuTUI ATCGAT 1 cut(s) 423
BtgI CCRYGG 4 cut(s) 46, 222, 392, 904
BtgZI GCGATG 1 cut(s) 52
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 6 cut(s) 239, 341, 468, 949, 1243, 1289
BtsIMutI CAGTG 4 cut(s) 36, 1158, 1551, 1692
CfoI GCGC 3 cut(s) 148, 413, 925
Cfr10I RCCGGY 8 cut(s) 74, 128, 179, 536, 776, 874, 880, 1232
Cfr13I GGNCC 7 cut(s) 359, 494, 901, 908, 995, 1261, 1495
Cfr9I CCCGGG 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 423
CseI GACGC 5 cut(s) 84, 109, 224, 296, 329
Csp6I GTAC 5 cut(s) 559, 796, 855, 1026, 1050
CspAI ACCGGT 2 cut(s) 776, 880
CviAII CATG 8 cut(s) 133, 393, 478, 691, 1219, 1238, 1514, 1654
CviQI GTAC 5 cut(s) 559, 796, 855, 1026, 1050
DdeI CTNAG 2 cut(s) 251, 813
DpnI GATC 4 cut(s) 25, 422, 426, 1135
DpnII GATC 4 cut(s) 23, 420, 424, 1133
DraIII CACNNNGTG 1 cut(s) 1696
DrdI GACNNNNNNGTC 1 cut(s) 743
DriI GACNNNNNGTC 1 cut(s) 1501
DseDI GACNNNNNNGTC 1 cut(s) 743
EaeI YGGCCR 4 cut(s) 43, 76, 225, 980
EagI CGGCCG 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 571
Eam1105I GACNNNNNGTC 1 cut(s) 1501
EarI CTCTTC 1 cut(s) 571
EciI GGCGGA 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 76
Eco130I CCWWGG 2 cut(s) 141, 392
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 591
Eco47I GGWCC 2 cut(s) 1261, 1495
Eco52I CGGCCG 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 508
Eco91I GGTNACC 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 1495
EcoO65I GGTNACC 1 cut(s) 376
EcoRII CCWGG 5 cut(s) 472, 600, 955, 977, 1264
EcoT14I CCWWGG 2 cut(s) 141, 392
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 2 cut(s) 141, 392
Esp3I CGTCTC 2 cut(s) 250, 340
FaeI CATG 8 cut(s) 136, 396, 481, 694, 1222, 1241, 1517, 1657
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 1617, 1649
FaqI GGGAC 1 cut(s) 1508
FatI CATG 8 cut(s) 132, 392, 477, 690, 1218, 1237, 1513, 1653
FauI CCCGC 5 cut(s) 254, 359, 485, 760, 1193
FblI GTMKAC 1 cut(s) 1460
FokI GGATG 6 cut(s) 246, 328, 455, 956, 1230, 1276
FriOI GRGCYC 1 cut(s) 372
FspBI CTAG 7 cut(s) 30, 152, 728, 1353, 1620, 1701, 1733
GlaI GCGC 3 cut(s) 147, 412, 924
HaeII RGCGCY 2 cut(s) 414, 926
HgaI GACGC 5 cut(s) 84, 109, 224, 296, 329
HhaI GCGC 3 cut(s) 148, 413, 925
Hin1I GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
Hin1II CATG 8 cut(s) 136, 396, 481, 694, 1222, 1241, 1517, 1657
Hin6I GCGC 3 cut(s) 146, 411, 923
HinP1I GCGC 3 cut(s) 146, 411, 923
HindIII AAGCTT 2 cut(s) 1565, 1631
HinfI GANTC 7 cut(s) 191, 465, 694, 861, 1117, 1193, 1402
Hpy166II GTNNAC 5 cut(s) 99, 532, 1067, 1165, 1461
Hpy188I TCNGA 8 cut(s) 196, 319, 455, 1015, 1294, 1407, 1476, 1681
Hpy188III TCNNGA 3 cut(s) 428, 590, 645
Hpy8I GTNNAC 5 cut(s) 99, 532, 1067, 1165, 1461
Hpy99I CGWCG 8 cut(s) 16, 125, 233, 242, 245, 410, 719, 767
HpyAV CCTTC 5 cut(s) 539, 575, 716, 1020, 1090
HpyCH4III ACNGT 8 cut(s) 376, 406, 719, 1162, 1216, 1480, 1552, 1696
HpyCH4IV ACGT 4 cut(s) 84, 240, 744, 1071
HpyCH4V TGCA 8 cut(s) 653, 701, 809, 974, 1314, 1371, 1555, 1723
HpyF3I CTNAG 2 cut(s) 251, 813
HpySE526I ACGT 4 cut(s) 84, 240, 744, 1071
Hsp92I GRCGYC 6 cut(s) 95, 120, 216, 240, 288, 321
Hsp92II CATG 8 cut(s) 136, 396, 481, 694, 1222, 1241, 1517, 1657
HspAI GCGC 3 cut(s) 146, 411, 923
KflI GGGWCCC 1 cut(s) 1495
KroI GCCGGC 5 cut(s) 74, 128, 179, 536, 874
KroNI GCCGGC 5 cut(s) 76, 130, 181, 538, 876
Kzo9I GATC 4 cut(s) 23, 420, 424, 1133
LmnI GCTCC 2 cut(s) 142, 302
Lsp1109I GCAGC 8 cut(s) 167, 552, 637, 793, 821, 986, 1111, 1567
LweI GCATC 2 cut(s) 224, 350
MaeI CTAG 7 cut(s) 30, 152, 728, 1353, 1620, 1701, 1733
MaeII ACGT 4 cut(s) 84, 240, 744, 1071
MalI GATC 4 cut(s) 25, 422, 426, 1135
MbiI CCGCTC 1 cut(s) 1200
MboI GATC 4 cut(s) 23, 420, 424, 1133
MboII GAAGA 3 cut(s) 494, 558, 682
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 6 cut(s) 636, 640, 1315, 1358, 1366, 1592
MlyI GAGTC 3 cut(s) 185, 1126, 1202
MmeI TCCRAC 2 cut(s) 219, 1344
MroNI GCCGGC 5 cut(s) 74, 128, 179, 536, 874
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 2 cut(s) 447, 639
MslI CAYNNNNRTG 1 cut(s) 1343
MspA1I CMGCKG 1 cut(s) 806
MvaI CCWGG 5 cut(s) 474, 602, 957, 979, 1266
MvnI CGCG 2 cut(s) 148, 767
NaeI GCCGGC 5 cut(s) 76, 130, 181, 538, 876
NciI CCSGG 6 cut(s) 363, 498, 509, 510, 1109, 1641
NcoI CCATGG 1 cut(s) 392
NdeII GATC 4 cut(s) 23, 420, 424, 1133
NgoMIV GCCGGC 5 cut(s) 74, 128, 179, 536, 874
NheI GCTAGC 1 cut(s) 151
NlaIII CATG 8 cut(s) 136, 396, 481, 694, 1222, 1241, 1517, 1657
NmeAIII GCCGAG 3 cut(s) 84, 317, 878
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 540
PdiI GCCGGC 5 cut(s) 76, 130, 181, 538, 876
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 4 cut(s) 465, 694, 861, 1402
PflFI GACNNNGTC 1 cut(s) 242
PinAI ACCGGT 2 cut(s) 776, 880
Ple19I CGATCG 2 cut(s) 423, 427
PleI GAGTC 3 cut(s) 185, 1125, 1201
PpsI GAGTC 3 cut(s) 185, 1125, 1201
PpuMI RGGWCCY 1 cut(s) 1495
PshAI GACNNNNGTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 1495
Psp6I CCWGG 5 cut(s) 472, 600, 955, 977, 1264
PspEI GGTNACC 1 cut(s) 376
PspGI CCWGG 5 cut(s) 472, 600, 955, 977, 1264
PspPI GGNCC 7 cut(s) 359, 494, 901, 908, 995, 1261, 1495
PspPPI RGGWCCY 1 cut(s) 1495
PstI CTGCAG 1 cut(s) 811
PsyI GACNNNGTC 1 cut(s) 242
PvuI CGATCG 2 cut(s) 423, 427
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 5 cut(s) 560, 797, 856, 1027, 1051
RsaNI GTAC 5 cut(s) 559, 796, 855, 1026, 1050
RseI CAYNNNNRTG 1 cut(s) 1343
SaqAI TTAA 2 cut(s) 447, 639
Sau3AI GATC 4 cut(s) 23, 420, 424, 1133
Sau96I GGNCC 7 cut(s) 359, 494, 901, 908, 995, 1261, 1495
SchI GAGTC 3 cut(s) 185, 1126, 1202
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 2 cut(s) 224, 350
SfcI CTRYAG 1 cut(s) 807
SgrAI CRCCGGYG 1 cut(s) 776
SinI GGWCC 2 cut(s) 1261, 1495
SmaI CCCGGG 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 1343
SpeI ACTAGT 2 cut(s) 727, 1700
Sse9I AATT 6 cut(s) 636, 640, 1315, 1358, 1366, 1592
SspMI CTAG 7 cut(s) 30, 152, 728, 1353, 1620, 1701, 1733
StyI CCWWGG 2 cut(s) 141, 392
TaaI ACNGT 8 cut(s) 376, 406, 719, 1162, 1216, 1480, 1552, 1696
TaiI ACGT 4 cut(s) 87, 243, 747, 1074
TaqI TCGA 6 cut(s) 22, 423, 714, 859, 1196, 1302
TasI AATT 6 cut(s) 636, 640, 1315, 1358, 1366, 1592
TatI WGTACW 1 cut(s) 795
TauI GCSGC 9 cut(s) 74, 128, 151, 209, 221, 360, 770, 1088, 1103
TfiI GAWTC 4 cut(s) 465, 694, 861, 1402
Tru1I TTAA 2 cut(s) 447, 639
Tru9I TTAA 2 cut(s) 447, 639
TscAI CASTG 4 cut(s) 43, 1165, 1551, 1699
TseI GCWGC 8 cut(s) 155, 540, 650, 806, 809, 974, 1124, 1555
TspDTI ATGAA 2 cut(s) 17, 1278
TspGWI ACGGA 3 cut(s) 29, 246, 854
TspMI CCCGGG 1 cut(s) 508
TspRI CASTG 4 cut(s) 43, 1165, 1551, 1699
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 1261, 1495
VspI ATTAAT 1 cut(s) 639
XmaI CCCGGG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 1460
XmnI GAANNNNTTC 1 cut(s) 640
XspI CTAG 7 cut(s) 30, 152, 728, 1353, 1620, 1701, 1733
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.