MD17G1152800.v1.1

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017066
Physical Location & Seq
Reverse (-)
14166859 .. 14169234
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1152800.v1.1.491

Sequence Viewer

Length: 2376 bp
ATGGCTTTCTCTCTCCTCCATCTTCTCCTCTCTCTCATCATCTCTGTCTCCTCCCTATCTAACTTCTCAGCTTCTTCTTCTTCTTCTTCTCCTGAAAATAATAATCAGAATGATGGTGGAAAAACCTTCATTGTTCAAGTCCAACCAGACTCCAAACCCTCCATTTTCCCAACCCACCATGACTGGTACTCCTCCTCCCTTTCCTCCCTCTCCTCCTCCTCCTCCTCCCAACCCCCAACTATTCTCCACACTTACTCTACCGTCTTCCACGGCTTCTCAGCCAAGCTCTCCCCTTCCCAAGCCGACCAACTCCAGTCCCTCAGCCACGTCATCAGTCTCATTCCAGAGCAAGTCCGCCACATCCACACCACCCGCTCCCCTGAATTCCTCGGCCTCCGAACCACGGACCCGGCAGGACTTCTCAAGGAGTCCGACTTTGGGTCCGACCTCGTCATCGGAGTCATCGACACCGGAATCTGGCCAGAACGCCAGTCCTTCCACGACCGAGAACTGGGTCCCATCCCCACCAAATGGAAAGGCAGCTGCGTCGCCGGGAAGGACTTTGCGGCGAACCTTTGCAACCGCAAGCTCATCGGTGCCCGTTTCTTCTCCGCCGGCTTCGAATCCACTAACGGCAAGATGAATGAGACCACGGAGTACCGCTCCCCCAGAGACTCCGACGGCCACGGGACTCACACTGCCTCGATCGCCGCCGGACGGTACGTTTTCCCGGCGTCTACTCTCGGGTACGCCAAGGGAGTCGCAGCCGGCATGGCTCCCAAGGCACGGCTCGCCGCGTACAAGGTCTGCTGGAGCGCTGGCTGCTACGACTCCGACATCCTCGCCGCCTTCGACTCCGCTGTCGCCGACGGCTGCGACGTCGTGTCCCTCAGTGTAGGTGGCGTCGTCGTCCCGTACTACCTCGACGCCATAGCCATTGGCGCGTTCGGAGCCTCTGACGCCGGTGTCTTCGTGTCCGCGTCCGCCGGTAACGGCGGTCCGGGAGGGCTCACTGTCACCAATGTGGCTCCATGGGTGACAACCGTCGGCGCGGGAACTATTGATCGGGACTTCCCGGCCGATGTGAAGCTCGGAAACGGAAGAACAATCCCGGGCATGAGTGTATATTCCGGGCCGGGTCTCGCAGCTGGTCGGATGTACCCTCTGGTTTACGCTGATAGCGAAGGCAGCGACGGTTACTCCTCGTCCTTATGTCTAGAAGGTTCTTTGAGTCAAGATTTGGTCAAAGGAAAGATCGTCGTATGCGACCGTGGCATTAATTCAAGAGCTGCGAAAGGCGATGTTGTGAGGAAAGCTGGAGGGGTTGGAATGATACTCGCCAATGGGGTTTTCGACGGTGAAGGACTTGTAGCAGACTGCCACGTGTTACCCGCCACCGCGGTTGGTGCCGCCACCGGAGACGAAATCAGAAGGTACATCACAGCTGCATCCAAGTCCAAATCGCCACCTACGGCTACAATTGTGTTTAAAGGGACCAGAATTCGGGTGCGTCCCGCTCCTGTCGTGGCTTCATTTTCTGCCCGCGGGCCGAACCCGCAAGCACCAGAGATATTGAAGCCGGACGTGATTGCGCCCGGGTTGAATATACTGGCAGCTTGGCCGGACAAGATTGGACCTTCCGGTGTTGCTTCGGATAAGAGAAATACGGAGTTTAACATACTCTCTGGGACATCAATGGCTTGTCCACACGTGTCTGGATTGGCTGCTTTGCTGAAAGCAGCCCATCCAGACTGGAGTCCAGCTGCTATTAGGTCTGCCCTGATGACCACGGCTTACACCGTGGACAACAGGGGCGAGACCATGCTGGACGAGTCCAGTGGCAATACATCAACAGTCATGGACTTTGGGGCAGGCCATGTTCATCCCCAAAAGGCAATGGACCCTGGATTGGTCTATGACATTACTTCTTATGATTATGTGAATTTCTTGTGCAACTACAATTATACCACTAAGAACATACAAACGGTCACTAGGAAGCTTGCAAATTGCAATGGGGCAAAGAGGGCTGGACATGCTGGGAACCTCAATTACCCTTCATTGTCTGTGGTGTTTCAGCAGTACGGGAAGCATAAGATGAATACGCATTTTATCCGGACCGTCACGAACGTGGGCGGCCCCAAGTCTGTCTATCAGGTGAGGATCAGTCCGCCGGCGGGGACGACAGTGACGGTGGAGCCGGAGAGGCTCGCGTTTAGGAGGGTTGGGCAGAAGTTGAATTTTCTTGTGAGGGTTCATGCTTTGGCAGTGAAGCTATCTCCTGGTAGCACTAGTGTGACGAGTGGTTCTATAGTTTGGTCAGATGGGAAGCACACCGTGACAAGTCCTCTGGTTGTGACAATGCAACAACCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

792

Amino Acids

83.22

Weight (kDa)

8.75

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 42 - 122 3.4e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 147 - 614 5.2e-49 Subtilase family
PA PF02225 387 - 476 1.1e-12 PA domain
fn3_6 PF17766 681 - 785 1.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 860, 965
AatII GACGTC 1 cut(s) 882
AccB1I GGYRCC 2 cut(s) 596, 1406
AccB7I CCANNNNNTGG 1 cut(s) 531
AccBSI CCGCTC 3 cut(s) 375, 663, 1517
AccI GTMKAC 1 cut(s) 737
AccII CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
AccIII TCCGGA 1 cut(s) 2112
AclWI GGATC 1 cut(s) 2168
AcoI YGGCCR 4 cut(s) 479, 682, 1077, 1619
AcsI RAATTY 4 cut(s) 383, 1500, 1942, 2236
AcvI CACGTG 2 cut(s) 1384, 1711
AcyI GRCGYC 5 cut(s) 734, 879, 903, 927, 960
AdeI CACNNNGTG 1 cut(s) 2335
AfaI GTAC 9 cut(s) 188, 659, 722, 749, 800, 917, 1160, 1436, 2081
AfeI AGCGCT 1 cut(s) 817
AflIII ACRYGT 3 cut(s) 1383, 1708, 1710
AgsI TTSAA 5 cut(s) 137, 1284, 1576, 1603, 2236
AhdI GACNNNNNGTC 2 cut(s) 439, 883
AhlI ACTAGT 1 cut(s) 2288
AjiI CACGTC 2 cut(s) 328, 1585
AjnI CCWGG 2 cut(s) 1903, 2278
AjuI GAANNNNNNNTTGG 2 cut(s) 1334, 1366
AleI CACNNNNGTG 3 cut(s) 1022, 2126, 2291
Alw26I GTCTC 7 cut(s) 52, 341, 641, 666, 1145, 1413, 1811
AlwI GGATC 1 cut(s) 2168
Ama87I CYCGRG 3 cut(s) 743, 1111, 1595
Aor13HI TCCGGA 1 cut(s) 2112
Aor51HI AGCGCT 1 cut(s) 817
AoxI GGCC 9 cut(s) 391, 479, 682, 1077, 1133, 1547, 1619, 1873, 2134
ApoI RAATTY 4 cut(s) 383, 1500, 1942, 2236
AseI ATTAAT 1 cut(s) 1278
AspLEI GCGC 4 cut(s) 818, 944, 1052, 1594
AsuHPI GGTGA 4 cut(s) 1009, 1048, 1370, 2167
AsuII TTCGAA 1 cut(s) 621
AvaI CYCGRG 3 cut(s) 743, 1111, 1595
AvaII GGWCC 8 cut(s) 406, 441, 515, 998, 1494, 1634, 1900, 2115
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 481
BanI GGYRCC 2 cut(s) 596, 1406
BanII GRGCYC 1 cut(s) 1011
BbrPI CACGTG 2 cut(s) 1384, 1711
BbsI GAAGAC 2 cut(s) 256, 961
BbvCI CCTCAGC 1 cut(s) 320
BccI CCATC 5 cut(s) 27, 107, 527, 1752, 2315
BceAI ACGGC 8 cut(s) 286, 649, 697, 803, 886, 1009, 1488, 1806
BcgI CGANNNNNNTGC 4 cut(s) 529, 563, 574, 608
BciT130I CCWGG 2 cut(s) 1905, 2280
BcoDI GTCTC 7 cut(s) 52, 341, 641, 666, 1145, 1413, 1811
BcuI ACTAGT 1 cut(s) 2288
BfaI CTAG 3 cut(s) 1217, 1992, 2289
BfmI CTRYAG 1 cut(s) 2307
BfoI RGCGCY 1 cut(s) 819
BglI GCCNNNNNGGC 2 cut(s) 773, 2203
Bme18I GGWCC 8 cut(s) 406, 441, 515, 998, 1494, 1634, 1900, 2115
BmeRI GACNNNNNGTC 2 cut(s) 439, 883
BmeT110I CYCGRG 3 cut(s) 743, 1111, 1595
BmgBI CACGTC 2 cut(s) 328, 1585
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 1 cut(s) 1457
BmuI ACTGGG 1 cut(s) 521
BoxI GACNNNNGTC 2 cut(s) 1043, 1755
BpiI GAAGAC 2 cut(s) 256, 961
BplI GAGNNNNNCTC 2 cut(s) 647, 679
BpmI CTGGAG 4 cut(s) 296, 832, 1338, 1774
Bpu10I CCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 407
BsaAI YACGTR 2 cut(s) 1384, 1711
BsaHI GRCGYC 5 cut(s) 734, 879, 903, 927, 960
BsaI GGTCTC 3 cut(s) 641, 1145, 1811
BsaWI WCCGGW 4 cut(s) 470, 1415, 1640, 2112
BsaXI ACNNNNNCTCC 6 cut(s) 173, 179, 203, 209, 1184, 1214
Bse118I RCCGGY 5 cut(s) 614, 767, 962, 986, 2170
Bse1I ACTGG 7 cut(s) 188, 313, 490, 516, 1614, 1757, 1836
Bse3DI GCAATG 2 cut(s) 1902, 2017
BseAI TCCGGA 1 cut(s) 2112
BseBI CCWGG 2 cut(s) 1905, 2280
BseGI GGATG 7 cut(s) 360, 519, 837, 1161, 1448, 1744, 1882
BseMI GCAATG 2 cut(s) 1902, 2017
BseMII CTCAG 4 cut(s) 81, 291, 334, 904
BseNI ACTGG 7 cut(s) 188, 313, 490, 516, 1614, 1757, 1836
BseSI GKGCMC 1 cut(s) 601
BseX3I CGGCCG 1 cut(s) 1077
BseYI CCCAGC 1 cut(s) 2036
Bsh1236I CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
Bsh1285I CGRYCG 4 cut(s) 505, 708, 1080, 1270
BshFI GGCC 9 cut(s) 393, 481, 684, 1079, 1135, 1549, 1621, 1875, 2136
BshNI GGYRCC 2 cut(s) 596, 1406
BsiEI CGRYCG 4 cut(s) 505, 708, 1080, 1270
BsiHKCI CYCGRG 3 cut(s) 743, 1111, 1595
BsmAI GTCTC 7 cut(s) 52, 341, 641, 666, 1145, 1413, 1811
BsmBI CGTCTC 1 cut(s) 1413
BsnI GGCC 9 cut(s) 393, 481, 684, 1079, 1135, 1549, 1621, 1875, 2136
Bso31I GGTCTC 3 cut(s) 641, 1145, 1811
BsoBI CYCGRG 3 cut(s) 743, 1111, 1595
Bsp119I TTCGAA 1 cut(s) 621
Bsp1286I GDGCHC 2 cut(s) 601, 1011
Bsp13I TCCGGA 1 cut(s) 2112
Bsp143I GATC 4 cut(s) 705, 1063, 1254, 2160
Bsp19I CCATGG 1 cut(s) 1031
BspANI GGCC 9 cut(s) 393, 481, 684, 1079, 1135, 1549, 1621, 1875, 2136
BspCNI CTCAG 4 cut(s) 80, 290, 333, 903
BspEI TCCGGA 1 cut(s) 2112
BspFNI CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
BspPI GGATC 1 cut(s) 2168
BspT104I TTCGAA 1 cut(s) 621
BspT107I GGYRCC 2 cut(s) 596, 1406
BspTNI GGTCTC 3 cut(s) 641, 1145, 1811
BsrBI CCGCTC 3 cut(s) 375, 663, 1517
BsrDI GCAATG 2 cut(s) 1902, 2017
BsrFI RCCGGY 5 cut(s) 614, 767, 962, 986, 2170
BsrI ACTGG 7 cut(s) 188, 313, 490, 516, 1614, 1757, 1836
BssAI RCCGGY 5 cut(s) 614, 767, 962, 986, 2170
BssMI GATC 4 cut(s) 705, 1063, 1254, 2160
BssNI GRCGYC 5 cut(s) 734, 879, 903, 927, 960
BssT1I CCWWGG 3 cut(s) 753, 780, 1031
Bst2UI CCWGG 2 cut(s) 1905, 2280
BstACI GRCGYC 5 cut(s) 734, 879, 903, 927, 960
BstBAI YACGTR 2 cut(s) 1384, 1711
BstBI TTCGAA 1 cut(s) 621
BstDEI CTNAG 5 cut(s) 67, 277, 320, 890, 1971
BstF5I GGATG 7 cut(s) 360, 519, 837, 1161, 1448, 1744, 1882
BstFNI CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 4 cut(s) 818, 944, 1052, 1594
BstKTI GATC 4 cut(s) 708, 1066, 1257, 2163
BstMAI GTCTC 7 cut(s) 52, 341, 641, 666, 1145, 1413, 1811
BstMBI GATC 4 cut(s) 705, 1063, 1254, 2160
BstMCI CGRYCG 4 cut(s) 505, 708, 1080, 1270
BstNI CCWGG 2 cut(s) 1905, 2280
BstNSI RCATGY 1 cut(s) 2036
BstPAI GACNNNNGTC 2 cut(s) 1043, 1755
BstSFI CTRYAG 1 cut(s) 2307
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
BstV2I GAAGAC 2 cut(s) 256, 961
BstZI CGGCCG 1 cut(s) 1077
BsuRI GGCC 9 cut(s) 393, 481, 684, 1079, 1135, 1549, 1621, 1875, 2136
BtgZI GCGATG 1 cut(s) 1314
BtrI CACGTC 2 cut(s) 328, 1585
BtsCI GGATG 7 cut(s) 360, 519, 837, 1161, 1448, 1744, 1882
BtsI GCAGTG 2 cut(s) 696, 2271
BtsIMutI CAGTG 6 cut(s) 696, 898, 1011, 1843, 2190, 2271
CfoI GCGC 4 cut(s) 818, 944, 1052, 1594
Cfr10I RCCGGY 5 cut(s) 614, 767, 962, 986, 2170
Cfr42I CCGCGG 2 cut(s) 1401, 1546
Cfr9I CCCGGG 2 cut(s) 1111, 1595
CpoI CGGWCCG 2 cut(s) 998, 2115
CseI GACGC 7 cut(s) 535, 723, 892, 935, 968, 969, 1499
Csp6I GTAC 9 cut(s) 187, 658, 721, 748, 799, 916, 1159, 1435, 2080
CspCI CAANNNNNGTGG 2 cut(s) 1384, 1419
CspI CGGWCCG 2 cut(s) 998, 2115
CviAII CATG 9 cut(s) 179, 772, 1032, 1117, 1822, 1858, 1877, 2033, 2255
CviQI GTAC 9 cut(s) 187, 658, 721, 748, 799, 916, 1159, 1435, 2080
DdeI CTNAG 5 cut(s) 67, 277, 320, 890, 1971
DpnI GATC 4 cut(s) 707, 1065, 1256, 2162
DpnII GATC 4 cut(s) 705, 1063, 1254, 2160
DraI TTTAAA 1 cut(s) 1489
DraIII CACNNNGTG 1 cut(s) 2335
DrdI GACNNNNNNGTC 2 cut(s) 860, 965
DriI GACNNNNNGTC 2 cut(s) 439, 883
DseDI GACNNNNNNGTC 2 cut(s) 860, 965
EaeI YGGCCR 4 cut(s) 479, 682, 1077, 1619
EagI CGGCCG 1 cut(s) 1077
Eam1105I GACNNNNNGTC 2 cut(s) 439, 883
EciI GGCGGA 4 cut(s) 344, 601, 973, 2157
EclXI CGGCCG 1 cut(s) 1077
Eco130I CCWWGG 3 cut(s) 753, 780, 1031
Eco24I GRGCYC 1 cut(s) 1011
Eco31I GGTCTC 3 cut(s) 641, 1145, 1811
Eco47I GGWCC 8 cut(s) 406, 441, 515, 998, 1494, 1634, 1900, 2115
Eco47III AGCGCT 1 cut(s) 817
Eco52I CGGCCG 1 cut(s) 1077
Eco72I CACGTG 2 cut(s) 1384, 1711
Eco88I CYCGRG 3 cut(s) 743, 1111, 1595
EcoO109I RGGNCCY 1 cut(s) 515
EcoRI GAATTC 2 cut(s) 383, 1500
EcoRII CCWGG 2 cut(s) 1903, 2278
EcoT14I CCWWGG 3 cut(s) 753, 780, 1031
EcoT38I GRGCYC 1 cut(s) 1011
ErhI CCWWGG 3 cut(s) 753, 780, 1031
Esp3I CGTCTC 1 cut(s) 1413
FaeI CATG 9 cut(s) 182, 775, 1035, 1120, 1825, 1861, 1880, 2036, 2258
FatI CATG 9 cut(s) 178, 771, 1031, 1116, 1821, 1857, 1876, 2032, 2254
FauI CCCGC 8 cut(s) 380, 1045, 1399, 1522, 1538, 1550, 1563, 2167
FblI GTMKAC 1 cut(s) 737
FokI GGATG 7 cut(s) 347, 506, 824, 1168, 1435, 1731, 1869
FriOI GRGCYC 1 cut(s) 1011
FspBI CTAG 3 cut(s) 1217, 1992, 2289
GlaI GCGC 4 cut(s) 817, 943, 1051, 1593
GsaI CCCAGC 1 cut(s) 2040
GsuI CTGGAG 4 cut(s) 296, 832, 1338, 1774
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 9 cut(s) 393, 481, 684, 1079, 1135, 1549, 1621, 1875, 2136
HgaI GACGC 7 cut(s) 535, 723, 892, 935, 968, 969, 1499
HhaI GCGC 4 cut(s) 818, 944, 1052, 1594
Hin1I GRCGYC 5 cut(s) 734, 879, 903, 927, 960
Hin1II CATG 9 cut(s) 182, 775, 1035, 1120, 1825, 1861, 1880, 2036, 2258
Hin6I GCGC 4 cut(s) 816, 942, 1050, 1592
HinP1I GCGC 4 cut(s) 816, 942, 1050, 1592
HindIII AAGCTT 1 cut(s) 1997
HphI GGTGA 4 cut(s) 1009, 1048, 1370, 2167
Hpy166II GTNNAC 4 cut(s) 738, 1171, 1706, 1804
Hpy8I GTNNAC 4 cut(s) 738, 1171, 1706, 1804
HpyCH4IV ACGT 7 cut(s) 327, 723, 879, 1383, 1584, 1710, 2127
HpyCH4V TGCA 6 cut(s) 579, 1448, 1953, 2003, 2010, 2362
HpyF3I CTNAG 5 cut(s) 67, 277, 320, 890, 1971
HpySE526I ACGT 7 cut(s) 327, 723, 879, 1383, 1584, 1710, 2127
Hsp92I GRCGYC 5 cut(s) 734, 879, 903, 927, 960
Hsp92II CATG 9 cut(s) 182, 775, 1035, 1120, 1825, 1861, 1880, 2036, 2258
HspAI GCGC 4 cut(s) 816, 942, 1050, 1592
KflI GGGWCCC 1 cut(s) 515
Kpn2I TCCGGA 1 cut(s) 2112
KroI GCCGGC 3 cut(s) 614, 767, 2170
KroNI GCCGGC 3 cut(s) 616, 769, 2172
KspI CCGCGG 2 cut(s) 1401, 1546
Kzo9I GATC 4 cut(s) 705, 1063, 1254, 2160
LmnI GCTCC 8 cut(s) 380, 668, 781, 813, 950, 1033, 1522, 2194
LweI GCATC 1 cut(s) 1457
MaeI CTAG 3 cut(s) 1217, 1992, 2289
MaeII ACGT 7 cut(s) 327, 723, 879, 1383, 1584, 1710, 2127
MalI GATC 4 cut(s) 707, 1065, 1256, 2162
MbiI CCGCTC 3 cut(s) 375, 663, 1517
MboI GATC 4 cut(s) 705, 1063, 1254, 2160
MfeI CAATTG 1 cut(s) 1479
MhlI GDGCHC 2 cut(s) 601, 1011
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 9 cut(s) 383, 1279, 1479, 1500, 1942, 1960, 2005, 2047, 2236
MluNI TGGCCA 1 cut(s) 481
MmeI TCCRAC 7 cut(s) 166, 456, 468, 702, 858, 1133, 1306
Mox20I TGGCCA 1 cut(s) 481
MreI CGCCGGCG 1 cut(s) 2170
MroI TCCGGA 1 cut(s) 2112
MroNI GCCGGC 3 cut(s) 614, 767, 2170
MscI TGGCCA 1 cut(s) 481
MseI TTAA 3 cut(s) 1278, 1488, 1674
MslI CAYNNNNRTG 3 cut(s) 1022, 2126, 2291
Msp20I TGGCCA 1 cut(s) 481
MspA1I CMGCKG 7 cut(s) 543, 860, 1148, 1400, 1445, 1545, 1763
MunI CAATTG 1 cut(s) 1479
MvaI CCWGG 2 cut(s) 1905, 2280
MvnI CGCG 7 cut(s) 797, 944, 980, 1052, 1400, 1545, 2210
NaeI GCCGGC 3 cut(s) 616, 769, 2172
NcoI CCATGG 1 cut(s) 1031
NdeII GATC 4 cut(s) 705, 1063, 1254, 2160
NgoMIV GCCGGC 3 cut(s) 614, 767, 2170
NlaIII CATG 9 cut(s) 182, 775, 1035, 1120, 1825, 1861, 1880, 2036, 2258
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 8 cut(s) 1015, 1036, 1987, 2119, 2185, 2293, 2335, 2353
NspI RCATGY 1 cut(s) 2036
NspV TTCGAA 1 cut(s) 621
OliI CACNNNNGTG 3 cut(s) 1022, 2126, 2291
PcsI WCGNNNNNNNCGW 5 cut(s) 462, 849, 876, 1179, 1263
PdiI GCCGGC 3 cut(s) 616, 769, 2172
PfeI GAWTC 2 cut(s) 474, 623
PflFI GACNNNGTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 531
PfoI TCCNGGA 1 cut(s) 1000
Ple19I CGATCG 1 cut(s) 708
PmaCI CACGTG 2 cut(s) 1384, 1711
PmlI CACGTG 2 cut(s) 1384, 1711
Ppu21I YACGTR 2 cut(s) 1384, 1711
PpuMI RGGWCCY 1 cut(s) 515
PshAI GACNNNNGTC 2 cut(s) 1043, 1755
PshBI ATTAAT 1 cut(s) 1278
Psp5II RGGWCCY 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 1903, 2278
PspCI CACGTG 2 cut(s) 1384, 1711
PspFI CCCAGC 1 cut(s) 2036
PspGI CCWGG 2 cut(s) 1903, 2278
PspPPI RGGWCCY 1 cut(s) 515
PsrI GAACNNNNNNTAC 2 cut(s) 2033, 2065
PsyI GACNNNGTC 1 cut(s) 449
PvuI CGATCG 1 cut(s) 708
PvuII CAGCTG 4 cut(s) 543, 1148, 1445, 1763
RsaI GTAC 9 cut(s) 188, 659, 722, 749, 800, 917, 1160, 1436, 2081
RsaNI GTAC 9 cut(s) 187, 658, 721, 748, 799, 916, 1159, 1435, 2080
RseI CAYNNNNRTG 3 cut(s) 1022, 2126, 2291
Rsr2I CGGWCCG 2 cut(s) 998, 2115
RsrII CGGWCCG 2 cut(s) 998, 2115
SacII CCGCGG 2 cut(s) 1401, 1546
SaqAI TTAA 3 cut(s) 1278, 1488, 1674
Sau3AI GATC 4 cut(s) 705, 1063, 1254, 2160
SduI GDGCHC 2 cut(s) 601, 1011
SfaNI GCATC 1 cut(s) 1457
SfcI CTRYAG 1 cut(s) 2307
Sfr303I CCGCGG 2 cut(s) 1401, 1546
SfuI TTCGAA 1 cut(s) 621
SgrAI CRCCGGYG 2 cut(s) 962, 2170
SgrBI CCGCGG 2 cut(s) 1401, 1546
SinI GGWCC 8 cut(s) 406, 441, 515, 998, 1494, 1634, 1900, 2115
SmaI CCCGGG 2 cut(s) 1113, 1597
SmiMI CAYNNNNRTG 3 cut(s) 1022, 2126, 2291
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 2288
Sse9I AATT 9 cut(s) 383, 1279, 1479, 1500, 1942, 1960, 2005, 2047, 2236
SspMI CTAG 3 cut(s) 1217, 1992, 2289
StyI CCWWGG 3 cut(s) 753, 780, 1031
TaiI ACGT 7 cut(s) 330, 726, 882, 1386, 1587, 1713, 2130
TaqI TCGA 6 cut(s) 465, 621, 704, 852, 924, 1353
TaqII GACCGA 1 cut(s) 519
TasI AATT 9 cut(s) 383, 1279, 1479, 1500, 1942, 1960, 2005, 2047, 2236
TauI GCSGC 6 cut(s) 569, 713, 797, 848, 1412, 2136
TfiI GAWTC 2 cut(s) 474, 623
Tru1I TTAA 3 cut(s) 1278, 1488, 1674
Tru9I TTAA 3 cut(s) 1278, 1488, 1674
TscAI CASTG 6 cut(s) 703, 898, 1018, 1843, 2190, 2271
TseFI GTSAC 8 cut(s) 1015, 1036, 1987, 2119, 2185, 2293, 2335, 2353
Tsp45I GTSAC 8 cut(s) 1015, 1036, 1987, 2119, 2185, 2293, 2335, 2353
TspDTI ATGAA 7 cut(s) 118, 656, 1521, 1871, 2046, 2111, 2243
TspGWI ACGGA 4 cut(s) 419, 668, 1113, 1682
TspMI CCCGGG 2 cut(s) 1111, 1595
TspRI CASTG 6 cut(s) 703, 898, 1018, 1843, 2190, 2271
Tth111I GACNNNGTC 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 531
VpaK11BI GGWCC 8 cut(s) 406, 441, 515, 998, 1494, 1634, 1900, 2115
VspI ATTAAT 1 cut(s) 1278
XapI RAATTY 4 cut(s) 383, 1500, 1942, 2236
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 1 cut(s) 2036
XmaI CCCGGG 2 cut(s) 1111, 1595
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 3 cut(s) 1217, 1992, 2289
ZraI GACGTC 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.