Subtilisin-like protease
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G14240 |
| fragaria_vesca | FvH4_6g35680 |
| malus_domestica | MD17G1152800.v1.1 |
| prunus_persica | Prupe.3G032200_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr2g0147671 |
| rosa_laevigata | RLG00000020269 |
| rosa_multiflora | Rmu_sc0000308.1_g000011 |
| rosa_roxburghii | Rroxscaffold_2G00099400 |
| rosa_rugosa | Rorug02G0400700 |
| rosa_samantha | Rh2AG458100 Rh2BG470800 Rh2CG445500 |
| rosa_wichuraiana | Rw2G037460 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 806 |
| AatII | GACGTC | 1 cut(s) | 828 |
| Acc36I | ACCTGC | 1 cut(s) | 1345 |
| AccB1I | GGYRCC | 1 cut(s) | 995 |
| AccI | GTMKAC | 2 cut(s) | 1749, 2045 |
| AclWI | GGATC | 1 cut(s) | 399 |
| AcoI | YGGCCR | 6 cut(s) | 513, 628, 661, 1565, 1668, 1819 |
| AcsI | RAATTY | 2 cut(s) | 2297, 2323 |
| AcuI | CTGAAG | 4 cut(s) | 45, 54, 1541, 1569 |
| AcyI | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| AdeI | CACNNNGTG | 1 cut(s) | 2281 |
| AfaI | GTAC | 7 cut(s) | 551, 566, 1145, 1382, 1441, 1636, 2520 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 423, 598, 732, 1098, 1501, 1855, 2089, 2120, 2194 |
| AflII | CTTAAG | 1 cut(s) | 2315 |
| AflIII | ACRYGT | 1 cut(s) | 1654 |
| AgeI | ACCGGT | 2 cut(s) | 1361, 1465 |
| AgsI | TTSAA | 8 cut(s) | 95, 445, 1230, 1435, 1621, 1681, 2201, 2411 |
| AhlI | ACTAGT | 2 cut(s) | 1312, 2285 |
| AjiI | CACGTC | 1 cut(s) | 1330 |
| AjnI | CCWGG | 4 cut(s) | 1540, 1849, 1926, 2224 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 366, 398 |
| Alw21I | GWGCWC | 2 cut(s) | 2356, 2511 |
| Alw26I | GTCTC | 6 cut(s) | 9, 612, 835, 925, 1091, 1757 |
| AlwI | GGATC | 1 cut(s) | 399 |
| Ama87I | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| ApeKI | GCWGC | 7 cut(s) | 740, 1125, 1352, 1391, 1559, 1709, 2485 |
| ApoI | RAATTY | 2 cut(s) | 2297, 2323 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2275, 2307 |
| AsiGI | ACCGGT | 2 cut(s) | 1361, 1465 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1225 |
| AspLEI | GCGC | 3 cut(s) | 303, 998, 1510 |
| AsuNHI | GCTAGC | 1 cut(s) | 736 |
| AvaI | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| AvaII | GGWCC | 3 cut(s) | 345, 1846, 2080 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 405, 438 |
| BalI | TGGCCA | 2 cut(s) | 515, 1821 |
| BanI | GGYRCC | 1 cut(s) | 995 |
| BanII | GRGCYC | 2 cut(s) | 957, 2511 |
| BauI | CACGAG | 2 cut(s) | 395, 520 |
| BbsI | GAAGAC | 3 cut(s) | 144, 202, 1288 |
| Bbv12I | GWGCWC | 2 cut(s) | 2356, 2511 |
| BbvCI | CCTCAGC | 1 cut(s) | 836 |
| BbvI | GCAGC | 7 cut(s) | 752, 1137, 1339, 1378, 1571, 1696, 2472 |
| BccI | CCATC | 8 cut(s) | 26, 659, 890, 1133, 1836, 2008, 2261, 2464 |
| BceAI | ACGGC | 7 cut(s) | 595, 643, 648, 649, 791, 1060, 1331 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 520, 554, 1962, 1996, 2039, 2073 |
| BciT130I | CCWGG | 4 cut(s) | 1542, 1851, 1928, 2226 |
| BclI | TGATCA | 2 cut(s) | 1177, 1932 |
| BcoDI | GTCTC | 6 cut(s) | 9, 612, 835, 925, 1091, 1757 |
| BcuI | ACTAGT | 2 cut(s) | 1312, 2285 |
| BfoI | RGCGCY | 2 cut(s) | 999, 1511 |
| BfrI | CTTAAG | 1 cut(s) | 2315 |
| BfuAI | ACCTGC | 1 cut(s) | 1345 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 433, 719 |
| BglII | AGATCT | 1 cut(s) | 2565 |
| BmcAI | AGTACT | 1 cut(s) | 2520 |
| Bme18I | GGWCC | 3 cut(s) | 345, 1846, 2080 |
| BmeT110I | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| BmgBI | CACGTC | 1 cut(s) | 1330 |
| BmsI | GCATC | 4 cut(s) | 689, 809, 1403, 1758 |
| BmtI | GCTAGC | 1 cut(s) | 740 |
| BoxI | GACNNNNGTC | 2 cut(s) | 459, 2072 |
| BpiI | GAAGAC | 3 cut(s) | 144, 202, 1288 |
| BpmI | CTGGAG | 1 cut(s) | 242 |
| Bpu10I | CCTNAGC | 1 cut(s) | 836 |
| BsaHI | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| BsaI | GGTCTC | 1 cut(s) | 1091 |
| BsaWI | WCCGGW | 4 cut(s) | 342, 416, 1361, 1465 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 632, 662, 815, 845, 903, 933 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 423, 598, 732, 1098, 1501, 1855, 2089, 2120, 2194 |
| Bse118I | RCCGGY | 6 cut(s) | 659, 713, 764, 1121, 1361, 1465 |
| Bse1I | ACTGG | 4 cut(s) | 146, 259, 464, 487 |
| Bse3DI | GCAATG | 3 cut(s) | 876, 1848, 1963 |
| BseBI | CCWGG | 4 cut(s) | 1542, 1851, 1928, 2226 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 423, 598, 732, 1098, 1501, 1855, 2089, 2120, 2194 |
| BseMI | GCAATG | 3 cut(s) | 876, 1848, 1963 |
| BseMII | CTCAG | 3 cut(s) | 132, 850, 915 |
| BseNI | ACTGG | 4 cut(s) | 146, 259, 464, 487 |
| BseRI | GAGGAG | 2 cut(s) | 1138, 1281 |
| BseX3I | CGGCCG | 1 cut(s) | 661 |
| BseXI | GCAGC | 7 cut(s) | 752, 1137, 1339, 1378, 1571, 1696, 2472 |
| BseYI | CCCAGC | 2 cut(s) | 359, 1036 |
| Bsh1285I | CGRYCG | 3 cut(s) | 664, 1012, 2483 |
| BshNI | GGYRCC | 1 cut(s) | 995 |
| BshTI | ACCGGT | 2 cut(s) | 1361, 1465 |
| BsiEI | CGRYCG | 3 cut(s) | 664, 1012, 2483 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2356, 2511 |
| BsiHKCI | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| BslFI | GGGAC | 3 cut(s) | 842, 2093, 2506 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 423, 598, 732, 1098, 1501, 1855, 2089, 2120, 2194 |
| BsmAI | GTCTC | 6 cut(s) | 9, 612, 835, 925, 1091, 1757 |
| BsmBI | CGTCTC | 3 cut(s) | 835, 925, 1757 |
| BsmFI | GGGAC | 3 cut(s) | 842, 2093, 2506 |
| BsmI | GAATGC | 2 cut(s) | 766, 1590 |
| Bso31I | GGTCTC | 1 cut(s) | 1091 |
| BsoBI | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| Bsp1286I | GDGCHC | 3 cut(s) | 957, 2356, 2511 |
| Bsp143I | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| Bsp19I | CCATGG | 2 cut(s) | 214, 977 |
| BspCNI | CTCAG | 3 cut(s) | 131, 849, 914 |
| BspMI | ACCTGC | 1 cut(s) | 1345 |
| BspOI | GCTAGC | 1 cut(s) | 740 |
| BspPI | GGATC | 1 cut(s) | 399 |
| BspT107I | GGYRCC | 1 cut(s) | 995 |
| BspTI | CTTAAG | 1 cut(s) | 2315 |
| BspTNI | GGTCTC | 1 cut(s) | 1091 |
| BsrDI | GCAATG | 3 cut(s) | 876, 1848, 1963 |
| BsrFI | RCCGGY | 6 cut(s) | 659, 713, 764, 1121, 1361, 1465 |
| BsrI | ACTGG | 4 cut(s) | 146, 259, 464, 487 |
| BssAI | RCCGGY | 6 cut(s) | 659, 713, 764, 1121, 1361, 1465 |
| BssMI | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| BssNI | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| BssSI | CACGAG | 2 cut(s) | 395, 520 |
| BssT1I | CCWWGG | 3 cut(s) | 214, 726, 977 |
| Bst2BI | CACGAG | 2 cut(s) | 395, 520 |
| Bst2UI | CCWGG | 4 cut(s) | 1542, 1851, 1928, 2226 |
| Bst6I | CTCTTC | 2 cut(s) | 1156, 2237 |
| BstACI | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| BstAFI | CTTAAG | 1 cut(s) | 2315 |
| BstDEI | CTNAG | 6 cut(s) | 55, 118, 458, 836, 901, 1917 |
| BstDSI | CCRYGG | 4 cut(s) | 214, 631, 977, 1489 |
| BstEII | GGTNACC | 2 cut(s) | 961, 982 |
| BstH2I | RGCGCY | 2 cut(s) | 999, 1511 |
| BstHHI | GCGC | 3 cut(s) | 303, 998, 1510 |
| BstKTI | GATC | 8 cut(s) | 394, 402, 611, 1180, 1721, 1935, 2109, 2568 |
| BstMAI | GTCTC | 6 cut(s) | 9, 612, 835, 925, 1091, 1757 |
| BstMBI | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| BstMCI | CGRYCG | 3 cut(s) | 664, 1012, 2483 |
| BstNI | CCWGG | 4 cut(s) | 1542, 1851, 1928, 2226 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 459, 2072 |
| BstPI | GGTNACC | 2 cut(s) | 961, 982 |
| BstV1I | GCAGC | 7 cut(s) | 752, 1137, 1339, 1378, 1571, 1696, 2472 |
| BstV2I | GAAGAC | 3 cut(s) | 144, 202, 1288 |
| BstX2I | RGATCY | 2 cut(s) | 391, 2565 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2027 |
| BstYI | RGATCY | 2 cut(s) | 391, 2565 |
| BstZI | CGGCCG | 1 cut(s) | 661 |
| BtgI | CCRYGG | 4 cut(s) | 214, 631, 977, 1489 |
| BtgZI | GCGATG | 1 cut(s) | 637 |
| BtrI | CACGTC | 1 cut(s) | 1330 |
| BtsI | GCAGTG | 1 cut(s) | 354 |
| BtsIMutI | CAGTG | 8 cut(s) | 139, 155, 348, 354, 494, 2070, 2142, 2277 |
| BveI | ACCTGC | 1 cut(s) | 1345 |
| CfoI | GCGC | 3 cut(s) | 303, 998, 1510 |
| Cfr10I | RCCGGY | 6 cut(s) | 659, 713, 764, 1121, 1361, 1465 |
| Cfr9I | CCCGGG | 2 cut(s) | 1093, 2118 |
| CseI | GACGC | 5 cut(s) | 481, 694, 829, 881, 914 |
| Csp6I | GTAC | 7 cut(s) | 550, 565, 1144, 1381, 1440, 1635, 2519 |
| CspAI | ACCGGT | 2 cut(s) | 1361, 1465 |
| CviQI | GTAC | 7 cut(s) | 550, 565, 1144, 1381, 1440, 1635, 2519 |
| DdeI | CTNAG | 6 cut(s) | 55, 118, 458, 836, 901, 1917 |
| DinI | GGCGCC | 1 cut(s) | 997 |
| DpnI | GATC | 8 cut(s) | 393, 401, 610, 1179, 1720, 1934, 2108, 2567 |
| DpnII | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| DraIII | CACNNNGTG | 1 cut(s) | 2281 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 806 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 806 |
| EaeI | YGGCCR | 6 cut(s) | 513, 628, 661, 1565, 1668, 1819 |
| EagI | CGGCCG | 1 cut(s) | 661 |
| Eam1104I | CTCTTC | 2 cut(s) | 1156, 2237 |
| EarI | CTCTTC | 2 cut(s) | 1156, 2237 |
| EciI | GGCGGA | 2 cut(s) | 214, 919 |
| Ecl136II | GAGCTC | 1 cut(s) | 2509 |
| EclXI | CGGCCG | 1 cut(s) | 661 |
| Eco130I | CCWWGG | 3 cut(s) | 214, 726, 977 |
| Eco24I | GRGCYC | 2 cut(s) | 957, 2511 |
| Eco31I | GGTCTC | 1 cut(s) | 1091 |
| Eco47I | GGWCC | 3 cut(s) | 345, 1846, 2080 |
| Eco52I | CGGCCG | 1 cut(s) | 661 |
| Eco53kI | GAGCTC | 1 cut(s) | 2509 |
| Eco57I | CTGAAG | 4 cut(s) | 45, 54, 1541, 1569 |
| Eco88I | CYCGRG | 4 cut(s) | 290, 428, 1093, 2118 |
| Eco91I | GGTNACC | 2 cut(s) | 961, 982 |
| EcoICRI | GAGCTC | 1 cut(s) | 2509 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1580, 2080 |
| EcoO65I | GGTNACC | 2 cut(s) | 961, 982 |
| EcoRII | CCWGG | 4 cut(s) | 1540, 1849, 1926, 2224 |
| EcoT14I | CCWWGG | 3 cut(s) | 214, 726, 977 |
| EcoT38I | GRGCYC | 2 cut(s) | 957, 2511 |
| EgeI | GGCGCC | 1 cut(s) | 997 |
| EheI | GGCGCC | 1 cut(s) | 997 |
| ErhI | CCWWGG | 3 cut(s) | 214, 726, 977 |
| Esp3I | CGTCTC | 3 cut(s) | 835, 925, 1757 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1158, 1190, 2202, 2234 |
| FaqI | GGGAC | 3 cut(s) | 842, 2093, 2506 |
| FauI | CCCGC | 4 cut(s) | 554, 839, 1070, 2467 |
| FbaI | TGATCA | 2 cut(s) | 1177, 1932 |
| FblI | GTMKAC | 2 cut(s) | 1749, 2045 |
| FriOI | GRGCYC | 2 cut(s) | 957, 2511 |
| GlaI | GCGC | 3 cut(s) | 302, 997, 1509 |
| GsaI | CCCAGC | 2 cut(s) | 363, 1040 |
| GsuI | CTGGAG | 1 cut(s) | 242 |
| HaeII | RGCGCY | 2 cut(s) | 999, 1511 |
| HgaI | GACGC | 5 cut(s) | 481, 694, 829, 881, 914 |
| HhaI | GCGC | 3 cut(s) | 303, 998, 1510 |
| Hin1I | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| Hin6I | GCGC | 3 cut(s) | 301, 996, 1508 |
| HinP1I | GCGC | 3 cut(s) | 301, 996, 1508 |
| HincII | GTYRAC | 1 cut(s) | 1750 |
| HindII | GTYRAC | 1 cut(s) | 1750 |
| HindIII | AAGCTT | 3 cut(s) | 2150, 2216, 2595 |
| Hpy166II | GTNNAC | 6 cut(s) | 348, 573, 1117, 1750, 1861, 2046 |
| Hpy188III | TCNNGA | 4 cut(s) | 290, 397, 1175, 1230 |
| Hpy8I | GTNNAC | 6 cut(s) | 348, 573, 1117, 1750, 1861, 2046 |
| HpyCH4IV | ACGT | 5 cut(s) | 825, 1329, 1637, 1656, 2361 |
| HpyCH4V | TGCA | 7 cut(s) | 1286, 1394, 1559, 1956, 2304, 2330, 2488 |
| HpyF3I | CTNAG | 6 cut(s) | 55, 118, 458, 836, 901, 1917 |
| HpySE526I | ACGT | 5 cut(s) | 825, 1329, 1637, 1656, 2361 |
| Hsp92I | GRCGYC | 5 cut(s) | 705, 825, 873, 906, 996 |
| HspAI | GCGC | 3 cut(s) | 301, 996, 1508 |
| KasI | GGCGCC | 1 cut(s) | 995 |
| KflI | GGGWCCC | 1 cut(s) | 2080 |
| KroI | GCCGGC | 3 cut(s) | 659, 713, 1121 |
| KroNI | GCCGGC | 3 cut(s) | 661, 715, 1123 |
| Ksp22I | TGATCA | 2 cut(s) | 1177, 1932 |
| Kzo9I | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| LmnI | GCTCC | 6 cut(s) | 261, 542, 727, 887, 896, 979 |
| Lsp1109I | GCAGC | 7 cut(s) | 752, 1137, 1339, 1378, 1571, 1696, 2472 |
| LweI | GCATC | 4 cut(s) | 689, 809, 1403, 1758 |
| MaeII | ACGT | 5 cut(s) | 825, 1329, 1637, 1656, 2361 |
| MalI | GATC | 8 cut(s) | 393, 401, 610, 1179, 1720, 1934, 2108, 2567 |
| MboI | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| MflI | RGATCY | 2 cut(s) | 391, 2565 |
| MhlI | GDGCHC | 3 cut(s) | 957, 2356, 2511 |
| MlsI | TGGCCA | 2 cut(s) | 515, 1821 |
| MluCI | AATT | 9 cut(s) | 1225, 1406, 1900, 1906, 1951, 1993, 2177, 2297, 2323 |
| MluNI | TGGCCA | 2 cut(s) | 515, 1821 |
| Mly113I | GGCGCC | 1 cut(s) | 996 |
| MlyI | GAGTC | 7 cut(s) | 158, 578, 770, 794, 1711, 1787, 2488 |
| MmeI | TCCRAC | 2 cut(s) | 124, 1079 |
| Mox20I | TGGCCA | 2 cut(s) | 515, 1821 |
| MroNI | GCCGGC | 3 cut(s) | 659, 713, 1121 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1225 |
| MscI | TGGCCA | 2 cut(s) | 515, 1821 |
| MseI | TTAA | 2 cut(s) | 1032, 2316 |
| MslI | CAYNNNNRTG | 2 cut(s) | 144, 1928 |
| Msp20I | TGGCCA | 2 cut(s) | 515, 1821 |
| MspA1I | CMGCKG | 1 cut(s) | 1391 |
| MspCI | CTTAAG | 1 cut(s) | 2315 |
| Mva1269I | GAATGC | 2 cut(s) | 766, 1590 |
| MvaI | CCWGG | 4 cut(s) | 1542, 1851, 1928, 2226 |
| NaeI | GCCGGC | 3 cut(s) | 661, 715, 1123 |
| NarI | GGCGCC | 1 cut(s) | 996 |
| NcoI | CCATGG | 2 cut(s) | 214, 977 |
| NdeII | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| NgoMIV | GCCGGC | 3 cut(s) | 659, 713, 1121 |
| NheI | GCTAGC | 1 cut(s) | 736 |
| NmeAIII | GCCGAG | 3 cut(s) | 315, 669, 902 |
| NmuCI | GTSAC | 8 cut(s) | 961, 982, 1013, 1364, 2065, 2101, 2131, 2281 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 795, 804, 822, 849, 1125 |
| PctI | GAATGC | 2 cut(s) | 766, 1590 |
| PdiI | GCCGGC | 3 cut(s) | 661, 715, 1123 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1225 |
| PfeI | GAWTC | 9 cut(s) | 74, 107, 374, 1050, 1221, 1279, 1446, 1987, 2393 |
| PflFI | GACNNNGTC | 1 cut(s) | 827 |
| PfoI | TCCNGGA | 1 cut(s) | 2224 |
| PinAI | ACCGGT | 2 cut(s) | 1361, 1465 |
| PleI | GAGTC | 7 cut(s) | 157, 577, 770, 794, 1710, 1786, 2488 |
| PluTI | GGCGCC | 1 cut(s) | 999 |
| PpsI | GAGTC | 7 cut(s) | 157, 577, 770, 794, 1710, 1786, 2488 |
| PpuMI | RGGWCCY | 1 cut(s) | 2080 |
| PshAI | GACNNNNGTC | 2 cut(s) | 459, 2072 |
| Psp124BI | GAGCTC | 1 cut(s) | 2511 |
| Psp5II | RGGWCCY | 1 cut(s) | 2080 |
| Psp6I | CCWGG | 4 cut(s) | 1540, 1849, 1926, 2224 |
| PspEI | GGTNACC | 2 cut(s) | 961, 982 |
| PspFI | CCCAGC | 2 cut(s) | 359, 1036 |
| PspGI | CCWGG | 4 cut(s) | 1540, 1849, 1926, 2224 |
| PspPPI | RGGWCCY | 1 cut(s) | 2080 |
| PsuI | RGATCY | 2 cut(s) | 391, 2565 |
| PsyI | GACNNNGTC | 1 cut(s) | 827 |
| PvuII | CAGCTG | 1 cut(s) | 1391 |
| RsaI | GTAC | 7 cut(s) | 551, 566, 1145, 1382, 1441, 1636, 2520 |
| RsaNI | GTAC | 7 cut(s) | 550, 565, 1144, 1381, 1440, 1635, 2519 |
| RseI | CAYNNNNRTG | 2 cut(s) | 144, 1928 |
| SacI | GAGCTC | 1 cut(s) | 2511 |
| SalI | GTCGAC | 1 cut(s) | 1748 |
| SaqAI | TTAA | 2 cut(s) | 1032, 2316 |
| Sau3AI | GATC | 8 cut(s) | 391, 399, 608, 1177, 1718, 1932, 2106, 2565 |
| ScaI | AGTACT | 1 cut(s) | 2520 |
| SchI | GAGTC | 7 cut(s) | 158, 578, 770, 794, 1711, 1787, 2488 |
| SduI | GDGCHC | 3 cut(s) | 957, 2356, 2511 |
| SfaNI | GCATC | 4 cut(s) | 689, 809, 1403, 1758 |
| SfoI | GGCGCC | 1 cut(s) | 997 |
| SinI | GGWCC | 3 cut(s) | 345, 1846, 2080 |
| SmaI | CCCGGG | 2 cut(s) | 1095, 2120 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 144, 1928 |
| SmlI | CTYRAG | 1 cut(s) | 2315 |
| SmoI | CTYRAG | 1 cut(s) | 2315 |
| SpeI | ACTAGT | 2 cut(s) | 1312, 2285 |
| Sse9I | AATT | 9 cut(s) | 1225, 1406, 1900, 1906, 1951, 1993, 2177, 2297, 2323 |
| SspDI | GGCGCC | 1 cut(s) | 995 |
| SstI | GAGCTC | 1 cut(s) | 2511 |
| StyI | CCWWGG | 3 cut(s) | 214, 726, 977 |
| TaiI | ACGT | 5 cut(s) | 828, 1332, 1640, 1659, 2364 |
| TaqI | TCGA | 9 cut(s) | 607, 798, 1008, 1299, 1444, 1749, 1796, 1887, 2364 |
| TasI | AATT | 9 cut(s) | 1225, 1406, 1900, 1906, 1951, 1993, 2177, 2297, 2323 |
| TatI | WGTACW | 2 cut(s) | 1380, 2518 |
| TauI | GCSGC | 8 cut(s) | 306, 561, 659, 713, 794, 945, 1673, 1688 |
| TfiI | GAWTC | 9 cut(s) | 74, 107, 374, 1050, 1221, 1279, 1446, 1987, 2393 |
| Tru1I | TTAA | 2 cut(s) | 1032, 2316 |
| Tru9I | TTAA | 2 cut(s) | 1032, 2316 |
| TscAI | CASTG | 8 cut(s) | 146, 162, 355, 361, 494, 2070, 2142, 2284 |
| TseFI | GTSAC | 8 cut(s) | 961, 982, 1013, 1364, 2065, 2101, 2131, 2281 |
| TseI | GCWGC | 7 cut(s) | 740, 1125, 1352, 1391, 1559, 1709, 2485 |
| Tsp45I | GTSAC | 8 cut(s) | 961, 982, 1013, 1364, 2065, 2101, 2131, 2281 |
| TspDTI | ATGAA | 6 cut(s) | 76, 602, 1863, 2255, 2385, 2421 |
| TspGWI | ACGGA | 4 cut(s) | 459, 614, 831, 1439 |
| TspMI | CCCGGG | 2 cut(s) | 1093, 2118 |
| TspRI | CASTG | 8 cut(s) | 146, 162, 355, 361, 494, 2070, 2142, 2284 |
| Tth111I | GACNNNGTC | 1 cut(s) | 827 |
| Vha464I | CTTAAG | 1 cut(s) | 2315 |
| VpaK11BI | GGWCC | 3 cut(s) | 345, 1846, 2080 |
| XapI | RAATTY | 2 cut(s) | 2297, 2323 |
| XmaI | CCCGGG | 2 cut(s) | 1093, 2118 |
| XmiI | GTMKAC | 2 cut(s) | 1749, 2045 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1225 |
| ZraI | GACGTC | 1 cut(s) | 826 |
| ZrmI | AGTACT | 1 cut(s) | 2520 |