AT3G15080

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013135
Physical Location & Seq
Forward (+)
5074216 .. 5076566
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G15080.1

Sequence Viewer

Length: 828 bp
ATGAGTTCTGATCTGAAGAGAAAGCCGAAGAAGAAGATAACTCCTAAACCTGTTCAATTAAACCCTAATTGGTCACTCCTCCAACAAAAACTGAAATCTGATTCTCATGGCAACACTCGAAAATCTTCAAACCCAGAAAATCCCAGCTCCATATTAGGGAAGCGAAAGGAGAGACCCGATTCGGAGGTTGATGTTCCTAAGATTAATCCCTTAGCTCCGGTTAACGACGATTCCAGTTTGACAGATGAAGTGGCTATGGACTGTGAAATGGTTGGTGTCAGTCAAGGAACCAAAAGTGCCCTTGGACGAGTTACCTTGGTAAATAAATGGGGAAATGTTCTATATGATGAGTTTGTCCGTCCAGTGGAACATGTTGTTGACTTTCGTACTTCTATTAGCGGGATTCGACCTCGAGATTTGAGAAAGGCCAAAGATTTCCGCGTTGCTCAAACCAAAGTAGCAGAGTTGATAAAGGGAAAGATTCTCGTGGGACACGCCTTGCATAATGATCTCAAGGCTTTGCTGCTAACTCACCCGAAAAAGGACATAAGGGACACAGGAGAATATCAGCCCTTTCTCAAGGGCAAAACAAGAAAGTCTCTGAAACATCTCGCATCTGAAATTCTAGGGGCTGACATTCAAAATGGAGAGCACTGCCCTATTGATGATGCAAGAGCTGCGATGATGCTTTACCAAAAGAACAGAAAAGAGTGGGAGAAAACTGTGAAAGACCAGACACGGATGTGGCTGAAACAAAAGAAGCGTAAGCCTAAGAAGAAAGCTAAATATGGGAACAACGCTTCCACCAATGACAATCCAATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

275

Amino Acids

31.13

Weight (kDa)

9.92

Isoelectric Point (pI)

22.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 84 - 231 1.1e-15 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 441
AciI CCGC 2 cut(s) 399, 439
AcsI RAATTY 1 cut(s) 621
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 3 cut(s) 156, 364, 541
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 56, 129, 641
AleI CACNNNNGTG 1 cut(s) 742
AloI GAACNNNNNNTCC 2 cut(s) 785, 817
AluBI AGCT 4 cut(s) 147, 215, 677, 782
AluI AGCT 4 cut(s) 147, 215, 677, 782
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 166, 603
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 1 cut(s) 426
ApeKI GCWGC 2 cut(s) 523, 677
ApoI RAATTY 1 cut(s) 621
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 301
BauI CACGAG 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 510, 664
BcoDI GTCTC 2 cut(s) 166, 603
BfaI CTAG 1 cut(s) 626
BisI GCNGC 2 cut(s) 524, 678
BlsI GCNGC 2 cut(s) 525, 679
BmeT110I CYCGRG 1 cut(s) 411
BmiI GGNNCC 1 cut(s) 289
BmsI GCATC 3 cut(s) 623, 658, 675
Bpu10I CCTNAGC 1 cut(s) 211
BpuEI CTTGAG 2 cut(s) 497, 563
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 301, 315
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 364, 541
Bse1I ACTGG 2 cut(s) 234, 362
BseDI CCNNGG 2 cut(s) 301, 315
BseGI GGATG 1 cut(s) 747
BseLI CCNNNNNNNGG 3 cut(s) 156, 364, 541
BseNI ACTGG 2 cut(s) 234, 362
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 2 cut(s) 510, 664
BseYI CCCAGC 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 441
BshFI GGCC 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 504, 566
BslI CCNNNNNNNGG 3 cut(s) 156, 364, 541
BsmAI GTCTC 2 cut(s) 166, 603
BsmFI GGGAC 2 cut(s) 504, 566
BsnI GGCC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 166
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 301, 654
Bsp143I GATC 2 cut(s) 10, 508
BspACI CCGC 2 cut(s) 399, 439
BspANI GGCC 1 cut(s) 428
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 166
BsrI ACTGG 2 cut(s) 234, 362
BssECI CCNNGG 2 cut(s) 301, 315
BssMI GATC 2 cut(s) 10, 508
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 2 cut(s) 301, 315
Bst2BI CACGAG 1 cut(s) 485
Bst4CI ACNGT 2 cut(s) 263, 724
Bst6I CTCTTC 1 cut(s) 11
BstAPI GCANNNNNTGC 1 cut(s) 677
BstDEI CTNAG 3 cut(s) 198, 211, 771
BstF5I GGATG 1 cut(s) 747
BstFNI CGCG 1 cut(s) 441
BstKTI GATC 2 cut(s) 13, 511
BstMAI GTCTC 2 cut(s) 166, 603
BstMBI GATC 2 cut(s) 10, 508
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNSI RCATGY 1 cut(s) 374
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 510, 664
BsuRI GGCC 1 cut(s) 428
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 1 cut(s) 747
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 369, 652
Csp6I GTAC 1 cut(s) 387
CviAII CATG 2 cut(s) 107, 371
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 3 cut(s) 198, 211, 771
DpnI GATC 2 cut(s) 12, 510
DpnII GATC 2 cut(s) 10, 508
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 301, 315
Eco31I GGTCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 411
EcoT14I CCWWGG 2 cut(s) 301, 315
ErhI CCWWGG 2 cut(s) 301, 315
FaeI CATG 2 cut(s) 110, 374
FaiI YATR 9 cut(s) 108, 152, 257, 343, 345, 372, 504, 548, 789
FaqI GGGAC 2 cut(s) 504, 566
FatI CATG 2 cut(s) 106, 370
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 2 cut(s) 524, 678
FokI GGATG 1 cut(s) 754
Fsp4HI GCNGC 2 cut(s) 524, 678
FspBI CTAG 1 cut(s) 626
GluI GCNGC 2 cut(s) 524, 678
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 1 cut(s) 428
HapII CCGG 1 cut(s) 218
Hin1II CATG 2 cut(s) 110, 374
HincII GTYRAC 2 cut(s) 223, 379
HindII GTYRAC 2 cut(s) 223, 379
HinfI GANTC 5 cut(s) 101, 179, 230, 403, 481
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 2 cut(s) 223, 379
Hpy188I TCNGA 6 cut(s) 10, 15, 100, 184, 603, 619
Hpy188III TCNNGA 1 cut(s) 413
Hpy8I GTNNAC 2 cut(s) 223, 379
Hpy99I CGWCG 1 cut(s) 230
HpyCH4III ACNGT 2 cut(s) 263, 724
HpyCH4V TGCA 2 cut(s) 502, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 3 cut(s) 198, 211, 771
Hsp92II CATG 2 cut(s) 110, 374
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 10, 508
LmnI GCTCC 2 cut(s) 152, 220
LpnPI CCDG 8 cut(s) 63, 147, 157, 231, 247, 375, 543, 746
Lsp1109I GCAGC 2 cut(s) 510, 664
LweI GCATC 3 cut(s) 623, 658, 675
MaeI CTAG 1 cut(s) 626
MaeIII GTNAC 2 cut(s) 72, 310
MalI GATC 2 cut(s) 12, 510
MboI GATC 2 cut(s) 10, 508
MboII GAAGA 6 cut(s) 28, 40, 43, 46, 117, 787
MfeI CAATTG 1 cut(s) 819
MhlI GDGCHC 2 cut(s) 301, 654
MluCI AATT 4 cut(s) 56, 67, 621, 819
MmeI TCCRAC 1 cut(s) 106
MnlI CCTC 3 cut(s) 89, 178, 420
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 3 cut(s) 59, 204, 222
MslI CAYNNNNRTG 1 cut(s) 742
MspI CCGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 819
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeII GATC 2 cut(s) 10, 508
NlaIII CATG 2 cut(s) 110, 374
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 742
PaeR7I CTCGAG 1 cut(s) 411
PciI ACATGT 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 5 cut(s) 101, 179, 230, 403, 481
PkrI GCNGC 2 cut(s) 525, 679
PscI ACATGT 1 cut(s) 370
PshBI ATTAAT 1 cut(s) 204
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 289
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 1 cut(s) 742
SaqAI TTAA 3 cut(s) 59, 204, 222
SatI GCNGC 2 cut(s) 524, 678
Sau3AI GATC 2 cut(s) 10, 508
SduI GDGCHC 2 cut(s) 301, 654
SetI ASST 8 cut(s) 52, 149, 189, 217, 317, 412, 679, 784
SfaNI GCATC 3 cut(s) 623, 658, 675
Sfr274I CTCGAG 1 cut(s) 411
SlaI CTCGAG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 742
SmlI CTYRAG 3 cut(s) 411, 512, 578
SmoI CTYRAG 3 cut(s) 411, 512, 578
Sse9I AATT 4 cut(s) 56, 67, 621, 819
SsiI CCGC 2 cut(s) 399, 439
SspMI CTAG 1 cut(s) 626
StyI CCWWGG 2 cut(s) 301, 315
TaaI ACNGT 2 cut(s) 263, 724
TaqI TCGA 3 cut(s) 118, 406, 412
TasI AATT 4 cut(s) 56, 67, 621, 819
TfiI GAWTC 5 cut(s) 101, 179, 230, 403, 481
Tru1I TTAA 3 cut(s) 59, 204, 222
Tru9I TTAA 3 cut(s) 59, 204, 222
TscAI CASTG 2 cut(s) 369, 659
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 2 cut(s) 523, 677
Tsp45I GTSAC 1 cut(s) 72
TspDTI ATGAA 1 cut(s) 261
TspGWI ACGGA 2 cut(s) 347, 754
TspRI CASTG 2 cut(s) 369, 659
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 1 cut(s) 621
XceI RCATGY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.