Prupe.4G172700_v2.0.a1

RNA exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013135
Physical Location & Seq
Forward (+)
10229927 .. 10233983
4057 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G172700.1

Sequence Viewer

Length: 786 bp
ATGAAGAAGAACCCTAATAAGCAGTTGCAGATAAACCCCAACTGGGCTCAACTCCAACAAAAGCTGAAGAGCCATGGCCCTAAACCTCCAGCAACTATCTTAGGGAAGCGGAAAGAGAGAACGGATGCAGAGTTGGATGATTCTCTATCTCACGTCAATCCTCTCACTCCTGTTAATGATGATTGCAGTGTGACAGATGAAGTAGCCATGGATTGTGAAATGGTTGGTGTCAGTCAAGGAAACAAAAGTGCCCTTGGACGTGTTACACTGGTAAATAAATGGGGTAATGTTATATATGATGAGTTTGTTCGCCCACTAGAACATGTTGTTGATTTTCGCACTCAAATAAGTGGCATTCGTCCCCGGGACTTGAGAAAAGCAAAGGATTTTCGAATTGTTCAGAAAAGAGTGGCAGAGTTGATTAAAGGACGAATTCTTGTGGGCCATGCATTGCGCAATGATCTTAAGGCCTTGCTTTTAAGTCATCCCAAGGAGGATTTGAGGGATACATTGGAGTATGAACCTTTTATGAGGGGGGAAGGGTCCAGAAGGGCACTTCGGCATCTTGCAGCTGAGTTTCTTGATGTTCAGATCCAAAATGGGGAGCACTGCCCAGTAGAAGATGCTCGCGCTGCAATGGTTCTTTATCAGAAAAACAAAAAAGCATGGGAAAAGAGAGTTAAAGATCAAATTAAACTCAAGCAGAAACAGAAGAAACGAAAGCCTAAAAAGAAGCGGAAGGAGGGAGATGATTTGAAAACTGACCTTGCAGAAACAGCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

262

Amino Acids

29.9

Weight (kDa)

9.74

Isoelectric Point (pI)

31.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 455
AccII CGCG 1 cut(s) 630
AciI CCGC 2 cut(s) 109, 736
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 432
AcuI CTGAAG 1 cut(s) 86
AfiI CCNNNNNNNGG 2 cut(s) 43, 601
AflII CTTAAG 1 cut(s) 464
AflIII ACRYGT 2 cut(s) 259, 322
AgsI TTSAA 1 cut(s) 757
AjiI CACGTC 2 cut(s) 154, 260
AluBI AGCT 2 cut(s) 64, 572
AluI AGCT 2 cut(s) 64, 572
Alw21I GWGCWC 1 cut(s) 609
AlwI GGATC 1 cut(s) 586
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 3 cut(s) 76, 442, 468
ApeKI GCWGC 2 cut(s) 569, 632
ApoI RAATTY 1 cut(s) 432
AspLEI GCGC 2 cut(s) 456, 632
AspS9I GGNCC 3 cut(s) 77, 442, 543
AsuC2I CCSGG 2 cut(s) 364, 365
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 363
AvaII GGWCC 1 cut(s) 543
BaeGI GKGCMC 2 cut(s) 253, 556
BanII GRGCYC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 2 cut(s) 581, 619
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 2 cut(s) 364, 365
BfaI CTAG 2 cut(s) 317, 784
BfrI CTTAAG 1 cut(s) 464
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 2 cut(s) 570, 633
BlsI GCNGC 2 cut(s) 571, 634
Bme1390I CCNGG 2 cut(s) 364, 365
Bme18I GGWCC 1 cut(s) 543
BmeT110I CYCGRG 1 cut(s) 363
BmgBI CACGTC 2 cut(s) 154, 260
BmgT120I GGNCC 3 cut(s) 77, 442, 543
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 364, 365
BmrI ACTGGG 2 cut(s) 52, 608
BmsI GCATC 3 cut(s) 115, 571, 613
BmuI ACTGGG 2 cut(s) 52, 608
BpmI CTGGAG 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 2 cut(s) 391, 683
BpuMI CCSGG 2 cut(s) 364, 365
BsaJI CCNNGG 6 cut(s) 73, 207, 253, 362, 363, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 601
Bse1I ACTGG 3 cut(s) 47, 273, 614
Bse3DI GCAATG 3 cut(s) 449, 463, 642
BseDI CCNNGG 6 cut(s) 73, 207, 253, 362, 363, 489
BseGI GGATG 4 cut(s) 130, 142, 484, 779
BseLI CCNNNNNNNGG 2 cut(s) 43, 601
BseMI GCAATG 3 cut(s) 449, 463, 642
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 3 cut(s) 47, 273, 614
BseSI GKGCMC 2 cut(s) 253, 556
BseXI GCAGC 2 cut(s) 581, 619
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 3 cut(s) 78, 444, 470
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 1 cut(s) 364
BslFI GGGAC 2 cut(s) 345, 380
BslI CCNNNNNNNGG 2 cut(s) 43, 601
BsmFI GGGAC 2 cut(s) 345, 380
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 3 cut(s) 78, 444, 470
BsoBI CYCGRG 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 4 cut(s) 49, 253, 556, 609
Bsp143I GATC 3 cut(s) 460, 591, 685
Bsp19I CCATGG 2 cut(s) 73, 207
BspACI CCGC 2 cut(s) 109, 736
BspANI GGCC 3 cut(s) 78, 444, 470
BspCNI CTCAG 1 cut(s) 565
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 1 cut(s) 586
BspQI GCTCTTC 1 cut(s) 62
BspT104I TTCGAA 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 464
BsrDI GCAATG 3 cut(s) 449, 463, 642
BsrI ACTGG 3 cut(s) 47, 273, 614
BssECI CCNNGG 6 cut(s) 73, 207, 253, 362, 363, 489
BssMI GATC 3 cut(s) 460, 591, 685
BssT1I CCWWGG 4 cut(s) 73, 207, 253, 489
Bst6I CTCTTC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 2 cut(s) 100, 573
BstDSI CCRYGG 2 cut(s) 73, 207
BstF5I GGATG 4 cut(s) 130, 142, 484, 779
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 2 cut(s) 456, 632
BstKTI GATC 3 cut(s) 463, 594, 688
BstMBI GATC 3 cut(s) 460, 591, 685
BstMWI GCNNNNNNNGC 2 cut(s) 632, 776
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 362, 363
BstSLI GKGCMC 2 cut(s) 253, 556
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 581, 619
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 3 cut(s) 78, 444, 470
BtgI CCRYGG 2 cut(s) 73, 207
BtrI CACGTC 2 cut(s) 154, 260
BtsCI GGATG 4 cut(s) 130, 142, 484, 779
BtsI GCAGTG 2 cut(s) 193, 607
BtsIMutI CAGTG 3 cut(s) 193, 266, 607
Cac8I GCNNGC 1 cut(s) 628
CfoI GCGC 2 cut(s) 456, 632
Cfr13I GGNCC 3 cut(s) 77, 442, 543
Cfr9I CCCGGG 1 cut(s) 363
CviAII CATG 5 cut(s) 74, 208, 323, 446, 666
CviJI RGCY 9 cut(s) 47, 64, 72, 78, 206, 444, 470, 572, 724
CviKI_1 RGCY 9 cut(s) 47, 64, 72, 78, 206, 444, 470, 572, 724
DdeI CTNAG 2 cut(s) 100, 573
DpnI GATC 3 cut(s) 462, 593, 687
DpnII GATC 3 cut(s) 460, 591, 685
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 4 cut(s) 73, 207, 253, 489
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 432
EcoT14I CCWWGG 4 cut(s) 73, 207, 253, 489
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 4 cut(s) 73, 207, 253, 489
FaeI CATG 5 cut(s) 77, 211, 326, 449, 669
FaqI GGGAC 2 cut(s) 345, 380
FatI CATG 5 cut(s) 73, 207, 322, 445, 665
Fnu4HI GCNGC 2 cut(s) 570, 633
FokI GGATG 4 cut(s) 137, 149, 471, 766
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 2 cut(s) 570, 633
FspBI CTAG 2 cut(s) 317, 784
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 2 cut(s) 455, 631
GluI GCNGC 2 cut(s) 570, 633
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 3 cut(s) 78, 444, 470
HapII CCGG 1 cut(s) 364
HhaI GCGC 2 cut(s) 456, 632
Hin1II CATG 5 cut(s) 77, 211, 326, 449, 669
Hin6I GCGC 2 cut(s) 454, 630
HinP1I GCGC 2 cut(s) 454, 630
HinfI GANTC 1 cut(s) 140
HpaII CCGG 1 cut(s) 364
Hpy188I TCNGA 3 cut(s) 402, 591, 651
Hpy188III TCNNGA 2 cut(s) 546, 581
HpyAV CCTTC 3 cut(s) 533, 543, 733
HpyCH4IV ACGT 2 cut(s) 153, 259
HpyCH4V TGCA 7 cut(s) 28, 128, 186, 449, 569, 635, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 776
HpyF3I CTNAG 2 cut(s) 100, 573
HpySE526I ACGT 2 cut(s) 153, 259
Hsp92II CATG 5 cut(s) 77, 211, 326, 449, 669
HspAI GCGC 2 cut(s) 454, 630
Kzo9I GATC 3 cut(s) 460, 591, 685
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 604
LpnPI CCDG 7 cut(s) 28, 102, 183, 254, 377, 559, 627
Lsp1109I GCAGC 2 cut(s) 581, 619
LweI GCATC 3 cut(s) 115, 571, 613
MaeI CTAG 2 cut(s) 317, 784
MaeII ACGT 2 cut(s) 153, 259
MaeIII GTNAC 2 cut(s) 190, 262
MalI GATC 3 cut(s) 462, 593, 687
MboI GATC 3 cut(s) 460, 591, 685
MboII GAAGA 5 cut(s) 16, 19, 79, 632, 724
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 4 cut(s) 49, 253, 556, 609
MluCI AATT 3 cut(s) 393, 432, 690
MmeI TCCRAC 2 cut(s) 79, 114
MnlI CCTC 6 cut(s) 96, 171, 487, 495, 525, 736
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 6 cut(s) 174, 423, 465, 479, 681, 693
MspA1I CMGCKG 1 cut(s) 572
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 1 cut(s) 364
MspR9I CCNGG 2 cut(s) 364, 365
Mva1269I GAATGC 1 cut(s) 354
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 632, 776
NciI CCSGG 2 cut(s) 364, 365
NcoI CCATGG 2 cut(s) 73, 207
NdeII GATC 3 cut(s) 460, 591, 685
NlaIII CATG 5 cut(s) 77, 211, 326, 449, 669
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 1 cut(s) 190
NsbI TGCGCA 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 326
NspV TTCGAA 1 cut(s) 391
PceI AGGCCT 1 cut(s) 470
PciI ACATGT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 62
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 571, 634
PscI ACATGT 1 cut(s) 322
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 3 cut(s) 77, 442, 543
PsuI RGATCY 1 cut(s) 591
PvuII CAGCTG 1 cut(s) 572
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 6 cut(s) 174, 423, 465, 479, 681, 693
SatI GCNGC 2 cut(s) 570, 633
Sau3AI GATC 3 cut(s) 460, 591, 685
Sau96I GGNCC 3 cut(s) 77, 442, 543
ScrFI CCNGG 2 cut(s) 364, 365
SduI GDGCHC 4 cut(s) 49, 253, 556, 609
SetI ASST 7 cut(s) 66, 88, 156, 262, 526, 574, 768
SfaNI GCATC 3 cut(s) 115, 571, 613
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 1 cut(s) 543
SmaI CCCGGG 1 cut(s) 365
SmlI CTYRAG 3 cut(s) 370, 464, 698
SmoI CTYRAG 3 cut(s) 370, 464, 698
Sse9I AATT 3 cut(s) 393, 432, 690
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 2 cut(s) 109, 736
SspMI CTAG 2 cut(s) 317, 784
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 362, 363
StyI CCWWGG 4 cut(s) 73, 207, 253, 489
TaiI ACGT 2 cut(s) 156, 262
TaqI TCGA 1 cut(s) 391
TasI AATT 3 cut(s) 393, 432, 690
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 6 cut(s) 174, 423, 465, 479, 681, 693
Tru9I TTAA 6 cut(s) 174, 423, 465, 479, 681, 693
TscAI CASTG 3 cut(s) 193, 273, 614
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 569, 632
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 17, 213, 534
TspGWI ACGGA 1 cut(s) 137
TspMI CCCGGG 1 cut(s) 363
TspRI CASTG 3 cut(s) 193, 273, 614
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 543
XapI RAATTY 1 cut(s) 432
XceI RCATGY 1 cut(s) 326
XmaI CCCGGG 1 cut(s) 363
XspI CTAG 2 cut(s) 317, 784
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.