FvH4_3g19101

RNA exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013135
Physical Location & Seq
Forward (+)
12302068 .. 12309092
7025 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19101.t1

Sequence Viewer

Length: 795 bp
ATGAAAAAGAAAGCTACGCAGTTTCAACTAAATCCCAACTGGGCTCAATTCCAAGAAGACCTCAAGAACCAGCCTAAGCCTTCAAGACCCTTTAGCAACTCTGACTCTAAAATCCCTAAAACCATCTTGGGGAAAAGGAAAGAGAGGGTGGATGATTCTCCGTCTCTGTTGAATGTTCTGGAACCTGTTAATGATGATTGCAGTGTGACAGATGAAATAGGCATGGATTGTGAAATGGTTGGTGTTGGTCTAGGAAATAAAAGTGCGCTTGGACGAGTTACACTGATAAATAAATGGGGGAACGTTATATACGATGAGTTTGTTCGCCCAGTAGAACGCGTTGTTGATTTCCGTACTCAAATAAGTGGCATTCGTTCCTGGGACTTGCGAAAAGCAAAGGATTTTCGAATAGTTCAGAAAAATGTCGCTGAGCTGATTAAAGGACGAATTCTTGTGGGCCATGCATTGCGCAATGATCTGAAGGCATTACTTCTGAGTCATCCCAAGGAGGACATGAGGGATACATCAGTGTATCAACCCTTTAGGAGGGAGGAAGGATCTGTAAGGGCGCTACGGCATCTTGCAGCTGAGTTTCTTGGTGTTGAAATCCAAAACGGGGAGCACTGTCCTGTAGAAGATGCTCGTGCTGCAATGCTGCTTTATCAGAAAAACAGACAAGCATGGGAAAAGAGCGTTAAACTTCAACTTAGTATTAAAGAGAAGCAGAAGAAACGCGGCAAGGGAAAGAAGAAGCGAAAAGAGGGGCCTGATTTGAAACGGGATCATGCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

265

Amino Acids

30.26

Weight (kDa)

9.72

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 74 - 221 2e-10 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 470
AccII CGCG 2 cut(s) 339, 735
AciI CCGC 1 cut(s) 735
AclI AACGTT 1 cut(s) 303
AclWI GGATC 2 cut(s) 565, 789
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 3 cut(s) 129, 546, 616
AflIII ACRYGT 1 cut(s) 337
AgsI TTSAA 6 cut(s) 26, 84, 172, 605, 704, 775
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 3 cut(s) 14, 433, 587
AluI AGCT 3 cut(s) 14, 433, 587
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 2 cut(s) 565, 789
AoxI GGCC 2 cut(s) 457, 764
ApeKI GCWGC 3 cut(s) 584, 647, 655
ApoI RAATTY 1 cut(s) 447
AspLEI GCGC 3 cut(s) 268, 471, 571
AspS9I GGNCC 2 cut(s) 457, 764
AsuII TTCGAA 1 cut(s) 406
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanII GRGCYC 1 cut(s) 46
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 3 cut(s) 596, 634, 642
BccI CCATC 1 cut(s) 131
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 379
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 630
BfoI RGCGCY 1 cut(s) 572
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 4 cut(s) 585, 648, 656, 736
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 4 cut(s) 586, 649, 657, 737
Bme1390I CCNGG 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 457, 764
BmiI GGNNCC 2 cut(s) 183, 765
BmrFI CCNGG 1 cut(s) 379
BmrI ACTGGG 2 cut(s) 49, 323
BmsI GCATC 2 cut(s) 586, 628
BmuI ACTGGG 2 cut(s) 49, 323
BpiI GAAGAC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 378, 504
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 546, 616
Bse1I ACTGG 2 cut(s) 44, 329
Bse3DI GCAATG 3 cut(s) 464, 478, 657
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 378, 504
BseGI GGATG 2 cut(s) 157, 499
BseLI CCNNNNNNNGG 3 cut(s) 129, 546, 616
BseMI GCAATG 3 cut(s) 464, 478, 657
BseMII CTCAG 3 cut(s) 420, 485, 579
BseNI ACTGG 2 cut(s) 44, 329
BseXI GCAGC 3 cut(s) 596, 634, 642
Bsh1236I CGCG 2 cut(s) 339, 735
BshFI GGCC 2 cut(s) 459, 766
BsiHKAI GWGCWC 1 cut(s) 624
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 3 cut(s) 129, 546, 616
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 2 cut(s) 459, 766
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 46, 624
Bsp143I GATC 3 cut(s) 475, 557, 781
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 1 cut(s) 735
BspANI GGCC 2 cut(s) 459, 766
BspCNI CTCAG 3 cut(s) 421, 486, 580
BspFNI CGCG 2 cut(s) 339, 735
BspLI GGNNCC 2 cut(s) 183, 765
BspPI GGATC 2 cut(s) 565, 789
BspT104I TTCGAA 1 cut(s) 406
BsrDI GCAATG 3 cut(s) 464, 478, 657
BsrI ACTGG 2 cut(s) 44, 329
BssECI CCNNGG 2 cut(s) 378, 504
BssMI GATC 3 cut(s) 475, 557, 781
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 626
BstBI TTCGAA 1 cut(s) 406
BstDEI CTNAG 5 cut(s) 75, 429, 494, 588, 707
BstENI CCTNNNNNAGG 1 cut(s) 544
BstF5I GGATG 2 cut(s) 157, 499
BstFNI CGCG 2 cut(s) 339, 735
BstH2I RGCGCY 1 cut(s) 572
BstHHI GCGC 3 cut(s) 268, 471, 571
BstKTI GATC 3 cut(s) 478, 560, 784
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 3 cut(s) 475, 557, 781
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 339, 735
BstV1I GCAGC 3 cut(s) 596, 634, 642
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 459, 766
BtsCI GGATG 2 cut(s) 157, 499
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 4 cut(s) 208, 281, 534, 622
CfoI GCGC 3 cut(s) 268, 471, 571
Cfr13I GGNCC 2 cut(s) 457, 764
Csp6I GTAC 1 cut(s) 354
CviAII CATG 5 cut(s) 223, 461, 514, 681, 785
CviJI RGCY 8 cut(s) 14, 44, 73, 79, 433, 459, 587, 766
CviKI_1 RGCY 8 cut(s) 14, 44, 73, 79, 433, 459, 587, 766
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 5 cut(s) 75, 429, 494, 588, 707
DpnI GATC 3 cut(s) 477, 559, 783
DpnII GATC 3 cut(s) 475, 557, 781
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 226, 464, 517, 684, 788
FaiI YATR 7 cut(s) 224, 308, 310, 462, 515, 682, 786
FaqI GGGAC 1 cut(s) 395
FatI CATG 5 cut(s) 222, 460, 513, 680, 784
Fnu4HI GCNGC 4 cut(s) 585, 648, 656, 736
FokI GGATG 2 cut(s) 164, 486
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 4 cut(s) 585, 648, 656, 736
FspBI CTAG 1 cut(s) 251
FspI TGCGCA 1 cut(s) 470
GlaI GCGC 3 cut(s) 267, 470, 570
GluI GCNGC 4 cut(s) 585, 648, 656, 736
HaeII RGCGCY 1 cut(s) 572
HaeIII GGCC 2 cut(s) 459, 766
HhaI GCGC 3 cut(s) 268, 471, 571
Hin1II CATG 5 cut(s) 226, 464, 517, 684, 788
Hin6I GCGC 3 cut(s) 266, 469, 569
HinP1I GCGC 3 cut(s) 266, 469, 569
HinfI GANTC 3 cut(s) 104, 155, 496
Hpy188I TCNGA 5 cut(s) 103, 417, 480, 495, 666
Hpy188III TCNNGA 3 cut(s) 64, 84, 179
HpyAV CCTTC 3 cut(s) 90, 475, 548
HpyCH4III ACNGT 1 cut(s) 626
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 5 cut(s) 201, 464, 584, 650, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 5 cut(s) 75, 429, 494, 588, 707
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 5 cut(s) 226, 464, 517, 684, 788
HspAI GCGC 3 cut(s) 266, 469, 569
Kzo9I GATC 3 cut(s) 475, 557, 781
LmnI GCTCC 1 cut(s) 619
LpnPI CCDG 9 cut(s) 25, 83, 164, 198, 342, 364, 391, 642, 780
Lsp1109I GCAGC 3 cut(s) 596, 634, 642
LweI GCATC 2 cut(s) 586, 628
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 205, 277
MalI GATC 3 cut(s) 477, 559, 783
MboI GATC 3 cut(s) 475, 557, 781
MboII GAAGA 4 cut(s) 68, 647, 739, 760
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 2 cut(s) 46, 624
MluCI AATT 2 cut(s) 47, 447
MluI ACGCGT 1 cut(s) 337
MlyI GAGTC 2 cut(s) 98, 505
MnlI CCTC 7 cut(s) 71, 138, 502, 510, 540, 544, 754
Mph1103I ATGCAT 1 cut(s) 466
MseI TTAA 4 cut(s) 189, 438, 696, 714
MspA1I CMGCKG 1 cut(s) 587
MspR9I CCNGG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 379
MvnI CGCG 2 cut(s) 339, 735
MwoI GCNNNNNNNGC 1 cut(s) 647
NdeII GATC 3 cut(s) 475, 557, 781
NlaIII CATG 5 cut(s) 226, 464, 517, 684, 788
NlaIV GGNNCC 2 cut(s) 183, 765
NmuCI GTSAC 1 cut(s) 205
NsbI TGCGCA 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 466
NspV TTCGAA 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 369
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 4 cut(s) 586, 649, 657, 737
PleI GAGTC 2 cut(s) 98, 504
PpsI GAGTC 2 cut(s) 98, 504
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 2 cut(s) 183, 765
PspPI GGNCC 2 cut(s) 457, 764
PsrI GAACNNNNNNTAC 2 cut(s) 293, 325
PsuI RGATCY 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SaqAI TTAA 4 cut(s) 189, 438, 696, 714
SatI GCNGC 4 cut(s) 585, 648, 656, 736
Sau3AI GATC 3 cut(s) 475, 557, 781
Sau96I GGNCC 2 cut(s) 457, 764
SchI GAGTC 2 cut(s) 98, 505
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 2 cut(s) 46, 624
SetI ASST 6 cut(s) 16, 63, 187, 306, 435, 589
SfaNI GCATC 2 cut(s) 586, 628
SfcI CTRYAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 406
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 47, 447
SsiI CCGC 1 cut(s) 735
SspMI CTAG 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 1 cut(s) 626
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 406
TasI AATT 2 cut(s) 47, 447
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 4 cut(s) 189, 438, 696, 714
Tru9I TTAA 4 cut(s) 189, 438, 696, 714
TscAI CASTG 4 cut(s) 208, 288, 534, 629
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 3 cut(s) 584, 647, 655
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 17, 228
TspGWI ACGGA 2 cut(s) 150, 341
TspRI CASTG 4 cut(s) 208, 288, 534, 629
XagI CCTNNNNNAGG 1 cut(s) 544
XapI RAATTY 1 cut(s) 447
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.