Rh5BG223100

RNA exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013135
Physical Location & Seq
Forward (+)
25855031 .. 25859112
4082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG223100.1

Sequence Viewer

Length: 576 bp
ATGAAAAAGAAAGCTACGCAGTTTCAACTAAACCCCAACTGGGCTCAATTCCAAGAAGCGCTCAAGAGCCAGCCAAAGCCTTCCAGACCCTTCAACAACTCTGACTCTAAAACCCCAAAAACCATCTTAGGGAAACGGAAAGAGAGAGTGGATGATTCTCCGTCTCTGTTGAATGTTCTAGAACCTGTTAATGATGATTGCAGTGTGACAGATGAAATAGCCATGGATTGTGAAATGGTCGGTGTCGGTCTAGGAAATAAAAGTGCCCTTGGACGAGTTACACTGATAAATAAATGGGGGAACGTTATATATGACGAGTTTGTTCGCCCAGTAGAACGTGTTGTTGATTTCCGAACTCAAATAAGTGGCATTCGTTCCTGGGACTTGCGAAAAGCAAAGGATTTTCGGATAGTTCAGAAAAGAGTGGCAGAGGTGATTAAAGGACGAATTCTTGTGGGCCATGCATTACGCAATGATCTGAAGGCGTTACTTCTGAGTCACCCCAAGGAAGACTTGAGAGATACTTCCGTTTATCAACCATTTAAGAGGTACTTGTTGTCTTTAATAACTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

191

Amino Acids

21.82

Weight (kDa)

9.72

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 73 - 159 5.8e-08 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 303
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 1 cut(s) 551
AfeI AGCGCT 1 cut(s) 60
AfiI CCNNNNNNNGG 2 cut(s) 40, 129
AflIII ACRYGT 1 cut(s) 337
AgsI TTSAA 3 cut(s) 26, 94, 172
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 168
Aor51HI AGCGCT 1 cut(s) 60
AoxI GGCC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 447
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 2 cut(s) 445, 491
BaeGI GKGCMC 1 cut(s) 268
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanII GRGCYC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 516
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 2 cut(s) 179, 251
BfoI RGCGCY 1 cut(s) 62
Bme1390I CCNGG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 379
BmrI ACTGGG 2 cut(s) 49, 323
BmuI ACTGGG 2 cut(s) 49, 323
BpiI GAAGAC 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 47, 535
BsaJI CCNNGG 4 cut(s) 222, 268, 378, 504
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 129
Bse1I ACTGG 2 cut(s) 44, 329
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 4 cut(s) 222, 268, 378, 504
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 2 cut(s) 40, 129
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 2 cut(s) 44, 329
BseSI GKGCMC 1 cut(s) 268
BshFI GGCC 1 cut(s) 459
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 40, 129
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 1 cut(s) 459
Bsp1286I GDGCHC 2 cut(s) 46, 268
Bsp143I GATC 1 cut(s) 475
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 2 cut(s) 44, 329
BssECI CCNNGG 4 cut(s) 222, 268, 378, 504
BssMI GATC 1 cut(s) 475
BssT1I CCWWGG 3 cut(s) 222, 268, 504
Bst2UI CCWGG 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 127, 494
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 478
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 475
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstSLI GKGCMC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 208, 281
Cac8I GCNNGC 1 cut(s) 71
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 457
Csp6I GTAC 1 cut(s) 550
CviAII CATG 2 cut(s) 223, 461
CviJI RGCY 7 cut(s) 14, 44, 69, 73, 79, 221, 459
CviKI_1 RGCY 7 cut(s) 14, 44, 69, 73, 79, 221, 459
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 2 cut(s) 127, 494
DpnI GATC 1 cut(s) 477
DpnII GATC 1 cut(s) 475
Eco130I CCWWGG 3 cut(s) 222, 268, 504
Eco24I GRGCYC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 3 cut(s) 222, 268, 504
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 3 cut(s) 222, 268, 504
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 2 cut(s) 226, 464
FaiI YATR 5 cut(s) 224, 308, 310, 312, 462
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 536, 568
FaqI GGGAC 1 cut(s) 395
FatI CATG 2 cut(s) 222, 460
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 46
FspBI CTAG 2 cut(s) 179, 251
GlaI GCGC 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 1 cut(s) 459
HhaI GCGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 226, 464
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 3 cut(s) 104, 155, 496
HphI GGTGA 2 cut(s) 445, 491
Hpy188I TCNGA 6 cut(s) 103, 353, 408, 417, 480, 495
Hpy188III TCNNGA 3 cut(s) 64, 84, 179
HpyAV CCTTC 3 cut(s) 90, 100, 475
HpyCH4IV ACGT 2 cut(s) 303, 337
HpyCH4V TGCA 2 cut(s) 201, 464
HpyF3I CTNAG 2 cut(s) 127, 494
HpySE526I ACGT 2 cut(s) 303, 337
Hsp92II CATG 2 cut(s) 226, 464
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 1 cut(s) 475
LpnPI CCDG 7 cut(s) 25, 83, 97, 198, 342, 364, 391
MaeI CTAG 2 cut(s) 179, 251
MaeII ACGT 2 cut(s) 303, 337
MaeIII GTNAC 4 cut(s) 205, 277, 486, 497
MalI GATC 1 cut(s) 477
MboI GATC 1 cut(s) 475
MboII GAAGA 1 cut(s) 521
MhlI GDGCHC 2 cut(s) 46, 268
MluCI AATT 2 cut(s) 47, 447
MlyI GAGTC 2 cut(s) 98, 505
MnlI CCTC 2 cut(s) 424, 540
Mph1103I ATGCAT 1 cut(s) 466
MseI TTAA 5 cut(s) 189, 438, 543, 563, 574
MspR9I CCNGG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 379
NcoI CCATGG 1 cut(s) 222
NdeII GATC 1 cut(s) 475
NlaIII CATG 2 cut(s) 226, 464
NmuCI GTSAC 2 cut(s) 205, 497
NsiI ATGCAT 1 cut(s) 466
PctI GAATGC 1 cut(s) 369
PfeI GAWTC 1 cut(s) 155
PleI GAGTC 2 cut(s) 98, 504
PpsI GAGTC 2 cut(s) 98, 504
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspPI GGNCC 1 cut(s) 457
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
SaqAI TTAA 5 cut(s) 189, 438, 543, 563, 574
Sau3AI GATC 1 cut(s) 475
Sau96I GGNCC 1 cut(s) 457
SchI GAGTC 2 cut(s) 98, 505
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 2 cut(s) 46, 268
SetI ASST 6 cut(s) 16, 187, 306, 340, 435, 551
SmlI CTYRAG 2 cut(s) 62, 514
SmoI CTYRAG 2 cut(s) 62, 514
Sse9I AATT 2 cut(s) 47, 447
SspMI CTAG 2 cut(s) 179, 251
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 3 cut(s) 222, 268, 504
TaiI ACGT 2 cut(s) 306, 340
TaqII GACCGA 1 cut(s) 236
TasI AATT 2 cut(s) 47, 447
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 5 cut(s) 189, 438, 543, 563, 574
Tru9I TTAA 5 cut(s) 189, 438, 543, 563, 574
TscAI CASTG 2 cut(s) 208, 288
TseFI GTSAC 2 cut(s) 205, 497
Tsp45I GTSAC 2 cut(s) 205, 497
TspDTI ATGAA 2 cut(s) 17, 228
TspGWI ACGGA 3 cut(s) 150, 151, 517
TspRI CASTG 2 cut(s) 208, 288
XapI RAATTY 1 cut(s) 447
XbaI TCTAGA 1 cut(s) 178
XspI CTAG 2 cut(s) 179, 251
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.