FvH4_3g19102

RNA exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013135
Physical Location & Seq
Forward (+)
12309412 .. 12313980
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19102.t1

Sequence Viewer

Length: 744 bp
ATGACAAGCCCTCTCTGCCCAAGCACAGCTACATATTCTTTCCCCAAAAACCCAAATCAGAAGCGCGCGTTTGTCTCACTAAGAAGAAGAAAGATGAAAAAGAAAGCTACGCAGTTTCAACTAAATCCCAACTGGGCTCAATTCCAAGAAGACCTCAAGAACCAGCCTAAGCCTTCAAGACCCTTTAGCAACTCTGACTCTAAAATCCCTAAAACCATCTTGGGGAAAAGGAAAGAGAGGGTGGATGATTCTCCGTCTCTGTTGAATGTTCTGGAACCTGTTAATGATGATTGCAGTGTGACAGATGAAATAGGCATGGATTGTGAAATGGTTGGTGTTGGTCTAGGAAATAAAAGTGCGCTTGGACGAGTTACACTGATAAATAAATGGGGGAACGTTATATACGATGAGTTTGTTCGCCCAGTAGAACGCGTTGTTGATTTCCGTACTCAAATAAGTGGCATTCGTTCCTGGGACTTGCGAAAAGCAAAGGATTTTCGAATAGTTCAGAAAAATGTCGCTGAGCTGATTAAAGGACGAATTCTTGTGGGCCATGCATTGCGCAATGATCTGAAGGCATTACTTCTGAGTCATCCCAAGGAGGACATGAGGGATACATCAGTGTATCAACCCTTTAGGAGGGAGGAAGGATCTGTAAGGGCGCTACGGCATCTTGCAGCTGAGTTTCTTGGTGTTGAAATCCAAAACGGGGAGCACTGTCCTGTTAGTATTCTGAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

248

Amino Acids

28.12

Weight (kDa)

9.74

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 105 - 197 1.5e-07 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 563
AccII CGCG 3 cut(s) 66, 68, 432
AclI AACGTT 1 cut(s) 396
AclWI GGATC 1 cut(s) 658
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 3 cut(s) 222, 639, 709
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 4 cut(s) 119, 177, 265, 698
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 4 cut(s) 29, 107, 526, 680
AluI AGCT 4 cut(s) 29, 107, 526, 680
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 2 cut(s) 79, 261
AlwI GGATC 1 cut(s) 658
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 1 cut(s) 540
AspLEI GCGC 5 cut(s) 66, 68, 361, 564, 664
AspS9I GGNCC 1 cut(s) 550
AsuII TTCGAA 1 cut(s) 499
BaeI ACNNNNGTAYC 2 cut(s) 606, 639
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 1 cut(s) 689
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 683
BciT130I CCWGG 1 cut(s) 472
BciVI GTATCC 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 79, 261
BfaI CTAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 665
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 1 cut(s) 678
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 679
Bme1390I CCNGG 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 472
BmrI ACTGGG 2 cut(s) 142, 416
BmsI GCATC 1 cut(s) 679
BmuI ACTGGG 2 cut(s) 142, 416
BpiI GAAGAC 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 471, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 639, 709
Bse1I ACTGG 2 cut(s) 137, 422
Bse3DI GCAATG 2 cut(s) 557, 571
BseBI CCWGG 1 cut(s) 472
BseDI CCNNGG 2 cut(s) 471, 597
BseGI GGATG 2 cut(s) 250, 592
BseLI CCNNNNNNNGG 3 cut(s) 222, 639, 709
BseMI GCAATG 2 cut(s) 557, 571
BseMII CTCAG 3 cut(s) 513, 578, 672
BseNI ACTGG 2 cut(s) 137, 422
BsePI GCGCGC 1 cut(s) 64
BseXI GCAGC 1 cut(s) 689
Bsh1236I CGCG 3 cut(s) 66, 68, 432
BshFI GGCC 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 717
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 3 cut(s) 222, 639, 709
BsmAI GTCTC 2 cut(s) 79, 261
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 1 cut(s) 552
Bsp119I TTCGAA 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 139, 717
Bsp143I GATC 2 cut(s) 568, 650
Bsp1720I GCTNAGC 1 cut(s) 522
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 3 cut(s) 514, 579, 673
BspFNI CGCG 3 cut(s) 66, 68, 432
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 1 cut(s) 658
BspT104I TTCGAA 1 cut(s) 499
BsrDI GCAATG 2 cut(s) 557, 571
BsrI ACTGG 2 cut(s) 137, 422
BssECI CCNNGG 2 cut(s) 471, 597
BssHII GCGCGC 1 cut(s) 64
BssMI GATC 2 cut(s) 568, 650
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 472
Bst4CI ACNGT 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 5 cut(s) 80, 168, 522, 587, 681
BstENI CCTNNNNNAGG 1 cut(s) 637
BstF5I GGATG 2 cut(s) 250, 592
BstFNI CGCG 3 cut(s) 66, 68, 432
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 5 cut(s) 66, 68, 361, 564, 664
BstKTI GATC 2 cut(s) 571, 653
BstMAI GTCTC 2 cut(s) 79, 261
BstMBI GATC 2 cut(s) 568, 650
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNI CCWGG 1 cut(s) 472
BstSCI CCNGG 1 cut(s) 470
BstUI CGCG 3 cut(s) 66, 68, 432
BstV1I GCAGC 1 cut(s) 689
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 1 cut(s) 552
BtsCI GGATG 2 cut(s) 250, 592
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 4 cut(s) 301, 374, 627, 715
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 5 cut(s) 66, 68, 361, 564, 664
Cfr13I GGNCC 1 cut(s) 550
Csp6I GTAC 1 cut(s) 447
CviAII CATG 3 cut(s) 316, 554, 607
CviJI RGCY 9 cut(s) 9, 29, 107, 137, 166, 172, 526, 552, 680
CviKI_1 RGCY 9 cut(s) 9, 29, 107, 137, 166, 172, 526, 552, 680
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 5 cut(s) 80, 168, 522, 587, 681
DpnI GATC 2 cut(s) 570, 652
DpnII GATC 2 cut(s) 568, 650
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 593
EcoNI CCTNNNNNAGG 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 597
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 3 cut(s) 319, 557, 610
FaiI YATR 6 cut(s) 34, 317, 401, 403, 555, 608
FaqI GGGAC 1 cut(s) 488
FatI CATG 3 cut(s) 315, 553, 606
Fnu4HI GCNGC 1 cut(s) 678
FokI GGATG 2 cut(s) 257, 579
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 678
FspBI CTAG 1 cut(s) 344
FspI TGCGCA 1 cut(s) 563
GlaI GCGC 5 cut(s) 65, 67, 360, 563, 663
GluI GCNGC 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 1 cut(s) 552
HhaI GCGC 5 cut(s) 66, 68, 361, 564, 664
Hin1II CATG 3 cut(s) 319, 557, 610
Hin6I GCGC 5 cut(s) 64, 66, 359, 562, 662
HinP1I GCGC 5 cut(s) 64, 66, 359, 562, 662
HinfI GANTC 3 cut(s) 197, 248, 589
Hpy188I TCNGA 6 cut(s) 60, 196, 510, 573, 588, 735
Hpy188III TCNNGA 3 cut(s) 157, 177, 272
HpyAV CCTTC 3 cut(s) 183, 568, 641
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 3 cut(s) 294, 557, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
HpyF3I CTNAG 5 cut(s) 80, 168, 522, 587, 681
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 3 cut(s) 319, 557, 610
HspAI GCGC 5 cut(s) 64, 66, 359, 562, 662
Kzo9I GATC 2 cut(s) 568, 650
LmnI GCTCC 1 cut(s) 712
LpnPI CCDG 8 cut(s) 118, 176, 257, 291, 435, 457, 484, 735
Lsp1109I GCAGC 1 cut(s) 689
LweI GCATC 1 cut(s) 679
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 2 cut(s) 298, 370
MalI GATC 2 cut(s) 570, 652
MboI GATC 2 cut(s) 568, 650
MboII GAAGA 3 cut(s) 96, 99, 161
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 139, 717
MluCI AATT 2 cut(s) 140, 540
MluI ACGCGT 1 cut(s) 430
MlyI GAGTC 2 cut(s) 191, 598
MnlI CCTC 7 cut(s) 21, 164, 231, 595, 603, 633, 637
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 2 cut(s) 282, 531
MspA1I CMGCKG 1 cut(s) 680
MspR9I CCNGG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 472
MvnI CGCG 3 cut(s) 66, 68, 432
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeII GATC 2 cut(s) 568, 650
NlaIII CATG 3 cut(s) 319, 557, 610
NlaIV GGNNCC 1 cut(s) 276
NmuCI GTSAC 1 cut(s) 298
NsbI TGCGCA 1 cut(s) 563
NsiI ATGCAT 1 cut(s) 559
NspV TTCGAA 1 cut(s) 499
PauI GCGCGC 1 cut(s) 64
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 679
PleI GAGTC 2 cut(s) 191, 597
PpsI GAGTC 2 cut(s) 191, 597
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 550
PsrI GAACNNNNNNTAC 2 cut(s) 386, 418
PsuI RGATCY 1 cut(s) 650
PteI GCGCGC 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SaqAI TTAA 2 cut(s) 282, 531
SatI GCNGC 1 cut(s) 678
Sau3AI GATC 2 cut(s) 568, 650
Sau96I GGNCC 1 cut(s) 550
SchI GAGTC 2 cut(s) 191, 598
ScrFI CCNGG 1 cut(s) 472
SduI GDGCHC 2 cut(s) 139, 717
SetI ASST 7 cut(s) 31, 109, 156, 280, 399, 528, 682
SfaNI GCATC 1 cut(s) 679
SfuI TTCGAA 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 2 cut(s) 140, 540
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 470
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 499
TasI AATT 2 cut(s) 140, 540
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 2 cut(s) 282, 531
Tru9I TTAA 2 cut(s) 282, 531
TscAI CASTG 4 cut(s) 301, 381, 627, 722
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 110, 321
TspGWI ACGGA 2 cut(s) 243, 434
TspRI CASTG 4 cut(s) 301, 381, 627, 722
XagI CCTNNNNNAGG 1 cut(s) 637
XapI RAATTY 1 cut(s) 540
XspI CTAG 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.