AT3G16300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014226
Physical Location & Seq
Reverse (-)
5525322 .. 5526212
891 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16300.1

Sequence Viewer

Length: 639 bp
ATGGCGAAAGCAGCAGAGCAGAAACAAAACCCGACCGTTGCGAGTGCAGAAGCGAAGCTTGATATCCGCGACGTGGTCAACACAGCCATCACTTACGGCGAAGATAACCGTCACGGCGGCGGGAAACGCAATGACGTGGCCATGTTTGTGCTAAGAGCCATGTGCATGGCCGTATCAACGGTGGCTGTTACGTTAATGGTAACAGCACGCGAGACTAGCATGACCACTCTCTATGGCTTCGAGTTTCAGCTCCACGCCGTTTGGTCTCTCTCAGATTCTCTTATATATCTAGTTGTGGTTTCATCGGCAACGGTTCTTTACTCGTTGATTCAACTGATTATAAGTGGAACAAGACTAATGAGAAAATCTCCGGTGATTCCGACAAGAACACAAGCATGGTTTTGCTTCGTTGCAGATCAGATAATAGGATACGCAATGGTGAGTGGAGGATCTGCAGCTTTAGGAGTGACGAATATGAACAGAACAGGAATCAGACACATGCCTCTTCCTAATTTCTGCAAATCTCTTGGTTTCTTCTGTGACCATCTCGCTGGCTCCATCGTCTTCGCCTTATTCGCGTTTCTCCTCCTCGCCGCTTCTTCACTTCTCGACGTTCTCCATCTCTCACGCCACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

212

Amino Acids

23.11

Weight (kDa)

9.21

Isoelectric Point (pI)

25.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 43 - 196 9.6e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014226)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79780 AT3G16300
fragaria_vesca FvH4_6g32160
malus_domestica MD09G1205900.v1.1
prunus_persica Prupe.3G061300_v2.0.a1 Prupe.3G065500_v2.0.a1
pyrus_communis pycom09g12270
rosa_chinensis RchiOBHm_Chr2g0140051
rosa_multiflora Rmu_sc0003365.1_g000011 Rmu_sc0012959.1_g000001
rosa_roxburghii Rroxscaffold_2G00105110
rosa_rugosa Rorug02G0360800
rosa_samantha Rh2AG411400 Rh2BG421600 Rh2CG397500 Rh2DG431100
rosa_wichuraiana Rw2G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 341
AccII CGCG 3 cut(s) 69, 210, 578
AciI CCGC 5 cut(s) 67, 117, 120, 594, 634
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 2 cut(s) 138, 168
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 1 cut(s) 332
AjiI CACGTC 2 cut(s) 73, 136
AluBI AGCT 3 cut(s) 58, 250, 458
AluI AGCT 3 cut(s) 58, 250, 458
Alw26I GTCTC 2 cut(s) 206, 270
AlwI GGATC 1 cut(s) 457
AoxI GGCC 2 cut(s) 138, 168
ApeKI GCWGC 2 cut(s) 11, 455
AsuHPI GGTGA 2 cut(s) 385, 451
BalI TGGCCA 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 23, 467
BccI CCATC 4 cut(s) 95, 552, 566, 627
BceAI ACGGC 4 cut(s) 112, 130, 155, 242
BciVI GTATCC 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 206, 270
BfaI CTAG 2 cut(s) 216, 290
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 422
BisI GCNGC 4 cut(s) 12, 118, 456, 594
BlsI GCNGC 4 cut(s) 13, 119, 457, 595
BmgBI CACGTC 2 cut(s) 73, 136
BmiI GGNNCC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BsaI GGTCTC 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse3DI GCAATG 2 cut(s) 136, 441
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMI GCAATG 2 cut(s) 136, 441
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 2 cut(s) 575, 578
BseXI GCAGC 2 cut(s) 23, 467
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 3 cut(s) 69, 210, 578
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 140, 170
BsiEI CGRYCG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 2 cut(s) 206, 270
BsnI GGCC 2 cut(s) 140, 170
Bso31I GGTCTC 1 cut(s) 270
Bsp143I GATC 2 cut(s) 415, 449
BspACI CCGC 5 cut(s) 67, 117, 120, 594, 634
BspANI GGCC 2 cut(s) 140, 170
BspCNI CTCAG 1 cut(s) 284
BspFNI CGCG 3 cut(s) 69, 210, 578
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 270
BsrDI GCAATG 2 cut(s) 136, 441
BssMI GATC 2 cut(s) 415, 449
Bst4CI ACNGT 4 cut(s) 37, 110, 181, 313
Bst6I CTCTTC 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 208, 553
BstDEI CTNAG 2 cut(s) 152, 271
BstFNI CGCG 3 cut(s) 69, 210, 578
BstKTI GATC 2 cut(s) 418, 452
BstMAI GTCTC 2 cut(s) 206, 270
BstMBI GATC 2 cut(s) 415, 449
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 11, 126, 216, 575
BstNSI RCATGY 1 cut(s) 502
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 3 cut(s) 69, 210, 578
BstV1I GCAGC 2 cut(s) 23, 467
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 449
BstXI CCANNNNNNTGG 2 cut(s) 166, 551
BstYI RGATCY 1 cut(s) 449
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 2 cut(s) 140, 170
BtrI CACGTC 2 cut(s) 73, 136
Cac8I GCNNGC 2 cut(s) 208, 553
CspCI CAANNNNNGTGG 2 cut(s) 242, 277
CviAII CATG 6 cut(s) 142, 160, 166, 220, 396, 499
DdeI CTNAG 2 cut(s) 152, 271
DpnI GATC 2 cut(s) 417, 451
DpnII GATC 2 cut(s) 415, 449
EaeI YGGCCR 2 cut(s) 138, 168
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 64
EcoRV GATATC 1 cut(s) 64
FaeI CATG 6 cut(s) 145, 163, 169, 223, 399, 502
FalI AAGNNNNNCTT 2 cut(s) 42, 74
FatI CATG 6 cut(s) 141, 159, 165, 219, 395, 498
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 4 cut(s) 12, 118, 456, 594
Fsp4HI GCNGC 4 cut(s) 12, 118, 456, 594
FspBI CTAG 2 cut(s) 216, 290
GluI GCNGC 4 cut(s) 12, 118, 456, 594
HaeIII GGCC 2 cut(s) 140, 170
HapII CCGG 1 cut(s) 371
Hin1II CATG 6 cut(s) 145, 163, 169, 223, 399, 502
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 275, 328, 376, 489
HpaII CCGG 1 cut(s) 371
HphI GGTGA 2 cut(s) 385, 451
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 4 cut(s) 274, 381, 420, 494
Hpy188III TCNNGA 1 cut(s) 608
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 74, 614
HpyCH4III ACNGT 4 cut(s) 37, 110, 181, 313
HpyCH4IV ACGT 4 cut(s) 72, 135, 191, 612
HpyCH4V TGCA 5 cut(s) 47, 165, 413, 455, 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 126, 216, 575
HpyF3I CTNAG 2 cut(s) 152, 271
HpySE526I ACGT 4 cut(s) 72, 135, 191, 612
Hsp92II CATG 6 cut(s) 145, 163, 169, 223, 399, 502
Kzo9I GATC 2 cut(s) 415, 449
LmnI GCTCC 2 cut(s) 255, 560
LpnPI CCDG 3 cut(s) 384, 471, 537
Lsp1109I GCAGC 2 cut(s) 23, 467
MaeI CTAG 2 cut(s) 216, 290
MaeII ACGT 4 cut(s) 72, 135, 191, 612
MaeIII GTNAC 5 cut(s) 110, 187, 199, 466, 539
MalI GATC 2 cut(s) 417, 451
MboI GATC 2 cut(s) 415, 449
MboII GAAGA 5 cut(s) 113, 497, 526, 556, 591
MflI RGATCY 1 cut(s) 449
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 1 cut(s) 511
MluNI TGGCCA 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 4 cut(s) 440, 513, 596, 599
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 1 cut(s) 194
MslI CAYNNNNRTG 3 cut(s) 146, 164, 394
Msp20I TGGCCA 1 cut(s) 140
MspI CCGG 1 cut(s) 371
MvnI CGCG 3 cut(s) 69, 210, 578
MwoI GCNNNNNNNGC 4 cut(s) 11, 126, 216, 575
NdeII GATC 2 cut(s) 415, 449
NlaIII CATG 6 cut(s) 145, 163, 169, 223, 399, 502
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 3 cut(s) 110, 466, 539
NspI RCATGY 1 cut(s) 502
PfeI GAWTC 4 cut(s) 275, 328, 376, 489
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 4 cut(s) 13, 119, 457, 595
PsiI TTATAA 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 556
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 449
PsyI GACNNNGTC 1 cut(s) 74
RseI CAYNNNNRTG 3 cut(s) 146, 164, 394
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 4 cut(s) 12, 118, 456, 594
Sau3AI GATC 2 cut(s) 415, 449
SetI ASST 7 cut(s) 60, 75, 138, 194, 252, 460, 615
SfcI CTRYAG 1 cut(s) 453
SmiMI CAYNNNNRTG 3 cut(s) 146, 164, 394
Sse9I AATT 1 cut(s) 511
SsiI CCGC 5 cut(s) 67, 117, 120, 594, 634
SspMI CTAG 2 cut(s) 216, 290
TaaI ACNGT 4 cut(s) 37, 110, 181, 313
TaiI ACGT 4 cut(s) 75, 138, 194, 615
TaqI TCGA 2 cut(s) 240, 609
TasI AATT 1 cut(s) 511
TauI GCSGC 2 cut(s) 120, 596
TfiI GAWTC 4 cut(s) 275, 328, 376, 489
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TseFI GTSAC 3 cut(s) 110, 466, 539
TseI GCWGC 2 cut(s) 11, 455
Tsp45I GTSAC 3 cut(s) 110, 466, 539
TspDTI ATGAA 2 cut(s) 291, 491
Tth111I GACNNNGTC 1 cut(s) 74
XceI RCATGY 1 cut(s) 502
XspI CTAG 2 cut(s) 216, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.