MD09G1205900.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014226
Physical Location & Seq
Forward (+)
19664213 .. 19666996
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1205900.v1.1.491

Sequence Viewer

Length: 630 bp
ATGATGAGCAGCCGAAAGACGACGTCGTTCGACGAAGAGGCGGCGGGGGTGGGAAAGGTGGTGGAGGAGGAGATCAATGGAACGGTGAGAAGTGGTGGCGCCACAAGTGTTTTATCTCGGAGAGCTGAAGTGACGCACTTGTGCCTGAGGGCGTTGTGCATGGCGGCCTCGGTGACGGCGCTGTCGTTCATGGTGACAGCCAGGGAGGCGACCACTGTGTACGTCTACGGATTTCCGCTCCCAGTCAACTCAAAGTGGTCCTTTGCCAACGCCTTCGAGTATCTGGTGGGAGTTTCAGCAGCTGTAGCAGCTCACTCATTGATGCAACTACTAATAAGTGTGTCTAGGCTGCTCAGGAAGTCTCCACTCATTCCCTCAAGGAATCATGCATGGCTTACTTTTGCTGGGGATCAGGTATTTGCATATGCTATGATAAGCGCAGGGTCAGCTGCATCAGGAGTAAGCAACCTGAACCGCAATGGAATCCGGCATACAGCTCTTCCGGATTTTTGTAAACCCTTGCATATTTTCTGTGACCATGTTGCAATCTCAATAGCCTTCACTTTCTTCAGTTGCCTCCTGCTGGCCTTATCTGCTGTCCAGAATGTAATTTGGCTCTCCAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

210

Amino Acids

22.51

Weight (kDa)

9.12

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 40 - 194 3.1e-39 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014226)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79780 AT3G16300
fragaria_vesca FvH4_6g32160
malus_domestica MD09G1205900.v1.1
prunus_persica Prupe.3G061300_v2.0.a1 Prupe.3G065500_v2.0.a1
pyrus_communis pycom09g12270
rosa_chinensis RchiOBHm_Chr2g0140051
rosa_multiflora Rmu_sc0003365.1_g000011 Rmu_sc0012959.1_g000001
rosa_roxburghii Rroxscaffold_2G00105110
rosa_rugosa Rorug02G0360800
rosa_samantha Rh2AG411400 Rh2BG421600 Rh2CG397500 Rh2DG431100
rosa_wichuraiana Rw2G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 5 cut(s) 41, 44, 164, 236, 475
AclWI GGATC 1 cut(s) 417
AcuI CTGAAG 2 cut(s) 147, 553
AcyI GRCGYC 2 cut(s) 23, 99
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 583
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 5 cut(s) 125, 302, 311, 449, 497
AluI AGCT 5 cut(s) 125, 302, 311, 449, 497
Alw26I GTCTC 1 cut(s) 366
AlwI GGATC 1 cut(s) 417
AlwNI CAGNNNCTG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 165, 585
ApeKI GCWGC 5 cut(s) 9, 299, 308, 349, 449
AspLEI GCGC 3 cut(s) 101, 181, 440
AspS9I GGNCC 1 cut(s) 258
AsuHPI GGTGA 3 cut(s) 97, 184, 205
AvaII GGWCC 1 cut(s) 258
AxyI CCTNAGG 1 cut(s) 146
BanI GGYRCC 1 cut(s) 98
BbvI GCAGC 5 cut(s) 21, 311, 320, 336, 436
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 102, 182
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 7 cut(s) 10, 42, 165, 300, 309, 350, 450
BlsI GCNGC 7 cut(s) 11, 43, 166, 301, 310, 351, 451
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 1 cut(s) 202
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 2 cut(s) 312, 461
BmuI ACTGGG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 361
BsaHI GRCGYC 2 cut(s) 23, 99
BsaJI CCNNGG 2 cut(s) 168, 201
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 1 cut(s) 583
Bse1I ACTGG 1 cut(s) 242
Bse21I CCTNAGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 484
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 2 cut(s) 168, 201
BseLI CCNNNNNNNGG 1 cut(s) 583
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 137, 367
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 2 cut(s) 80, 83
BseXI GCAGC 5 cut(s) 21, 311, 320, 336, 436
BseYI CCCAGC 1 cut(s) 404
BshFI GGCC 2 cut(s) 167, 587
BshNI GGYRCC 1 cut(s) 98
BsiSI CCGG 2 cut(s) 487, 503
BslI CCNNNNNNNGG 1 cut(s) 583
BsmAI GTCTC 1 cut(s) 366
BsnI GGCC 2 cut(s) 167, 587
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 2 cut(s) 72, 409
BspACI CCGC 5 cut(s) 41, 44, 164, 236, 475
BspANI GGCC 2 cut(s) 167, 587
BspCNI CTCAG 2 cut(s) 138, 366
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 100
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 484
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 168, 201
BssMI GATC 2 cut(s) 72, 409
BssNI GRCGYC 2 cut(s) 23, 99
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 2 cut(s) 85, 217
Bst6I CTCTTC 2 cut(s) 30, 504
BstACI GRCGYC 2 cut(s) 23, 99
BstC8I GCNNGC 1 cut(s) 585
BstDEI CTNAG 2 cut(s) 146, 353
BstH2I RGCGCY 2 cut(s) 102, 182
BstHHI GCGC 3 cut(s) 101, 181, 440
BstKTI GATC 2 cut(s) 75, 412
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 2 cut(s) 72, 409
BstMWI GCNNNNNNNGC 5 cut(s) 206, 305, 308, 446, 593
BstNI CCWGG 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 5 cut(s) 21, 311, 320, 336, 436
Bsu36I CCTNAGG 1 cut(s) 146
BsuRI GGCC 2 cut(s) 167, 587
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 585
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 3 cut(s) 101, 181, 440
Cfr13I GGNCC 1 cut(s) 258
CseI GACGC 1 cut(s) 142
Csp6I GTAC 1 cut(s) 220
CviAII CATG 5 cut(s) 160, 190, 386, 390, 539
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 2 cut(s) 146, 353
DinI GGCGCC 1 cut(s) 100
DpnI GATC 2 cut(s) 74, 411
DpnII GATC 2 cut(s) 72, 409
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 30, 504
EarI CTCTTC 2 cut(s) 30, 504
Eco47I GGWCC 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 147, 553
Eco81I CCTNAGG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 391
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
FaeI CATG 5 cut(s) 163, 193, 389, 393, 542
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 5 cut(s) 159, 189, 385, 389, 538
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 424
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 7 cut(s) 10, 42, 165, 300, 309, 350, 450
Fsp4HI GCNGC 7 cut(s) 10, 42, 165, 300, 309, 350, 450
FspBI CTAG 1 cut(s) 345
GlaI GCGC 3 cut(s) 100, 180, 439
GluI GCNGC 7 cut(s) 10, 42, 165, 300, 309, 350, 450
GsaI CCCAGC 1 cut(s) 408
HaeII RGCGCY 2 cut(s) 102, 182
HaeIII GGCC 2 cut(s) 167, 587
HapII CCGG 2 cut(s) 487, 503
HgaI GACGC 1 cut(s) 142
HhaI GCGC 3 cut(s) 101, 181, 440
Hin1I GRCGYC 2 cut(s) 23, 99
Hin1II CATG 5 cut(s) 163, 193, 389, 393, 542
Hin6I GCGC 3 cut(s) 99, 179, 438
HinP1I GCGC 3 cut(s) 99, 179, 438
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 382, 483
HpaII CCGG 2 cut(s) 487, 503
HphI GGTGA 3 cut(s) 97, 184, 205
Hpy166II GTNNAC 4 cut(s) 220, 226, 247, 515
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 355, 456, 503, 601
Hpy8I GTNNAC 4 cut(s) 220, 226, 247, 515
Hpy99I CGWCG 3 cut(s) 25, 28, 35
HpyAV CCTTC 2 cut(s) 283, 568
HpyCH4III ACNGT 2 cut(s) 85, 217
HpyCH4IV ACGT 2 cut(s) 23, 222
HpyCH4V TGCA 7 cut(s) 159, 325, 389, 422, 452, 523, 545
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 305, 308, 446, 593
HpyF3I CTNAG 2 cut(s) 146, 353
HpySE526I ACGT 2 cut(s) 23, 222
Hsp92I GRCGYC 2 cut(s) 23, 99
Hsp92II CATG 5 cut(s) 163, 193, 389, 393, 542
HspAI GCGC 3 cut(s) 99, 179, 438
KasI GGCGCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 2 cut(s) 72, 409
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 5 cut(s) 21, 311, 320, 336, 436
LweI GCATC 2 cut(s) 312, 461
MaeI CTAG 1 cut(s) 345
MaeII ACGT 2 cut(s) 23, 222
MaeIII GTNAC 4 cut(s) 130, 172, 193, 533
MalI GATC 2 cut(s) 74, 411
MbiI CCGCTC 1 cut(s) 238
MboI GATC 2 cut(s) 72, 409
MboII GAAGA 3 cut(s) 47, 491, 559
MluCI AATT 1 cut(s) 609
Mly113I GGCGCC 1 cut(s) 99
MnlI CCTC 8 cut(s) 31, 58, 61, 141, 178, 199, 385, 587
Mph1103I ATGCAT 1 cut(s) 391
MroI TCCGGA 1 cut(s) 502
MspA1I CMGCKG 2 cut(s) 302, 449
MspI CCGG 2 cut(s) 487, 503
MspR9I CCNGG 1 cut(s) 202
MvaI CCWGG 1 cut(s) 202
MwoI GCNNNNNNNGC 5 cut(s) 206, 305, 308, 446, 593
NarI GGCGCC 1 cut(s) 99
NdeI CATATG 1 cut(s) 424
NdeII GATC 2 cut(s) 72, 409
NlaIII CATG 5 cut(s) 163, 193, 389, 393, 542
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 4 cut(s) 130, 172, 193, 533
NsiI ATGCAT 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PfeI GAWTC 2 cut(s) 382, 483
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 7 cut(s) 11, 43, 166, 301, 310, 351, 451
PluTI GGCGCC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 200
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 302
PsyI GACNNNGTC 1 cut(s) 22
PvuII CAGCTG 2 cut(s) 302, 449
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SapI GCTCTTC 1 cut(s) 504
SatI GCNGC 7 cut(s) 10, 42, 165, 300, 309, 350, 450
Sau3AI GATC 2 cut(s) 72, 409
Sau96I GGNCC 1 cut(s) 258
ScrFI CCNGG 1 cut(s) 202
SfaNI GCATC 2 cut(s) 312, 461
SfcI CTRYAG 1 cut(s) 303
SfoI GGCGCC 1 cut(s) 100
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 1 cut(s) 609
SsiI CCGC 5 cut(s) 41, 44, 164, 236, 475
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 2 cut(s) 85, 217
TaiI ACGT 2 cut(s) 26, 225
TaqI TCGA 2 cut(s) 30, 276
TasI AATT 1 cut(s) 609
TauI GCSGC 2 cut(s) 44, 167
TfiI GAWTC 2 cut(s) 382, 483
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 4 cut(s) 130, 172, 193, 533
TseI GCWGC 5 cut(s) 9, 299, 308, 349, 449
Tsp45I GTSAC 4 cut(s) 130, 172, 193, 533
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 1 cut(s) 220
Tth111I GACNNNGTC 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 345
ZraI GACGTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.