AT3G18860

Transducin family protein WD-40 repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009603
Physical Location & Seq
Forward (+)
6501668 .. 6508553
6886 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18860.2

Sequence Viewer

Length: 2283 bp
ATGGTCATGGATATCGATTTCAACGAGTACAAGCTCAGGTGCGAGCTTCATGGCCACGATGATGATGTTCGTGGGATTTGTGTGTGTAATGATGAGAATATAGCTACTTCATCAAGAGATAGGACCATAAGGGTTTGGTCACTGGATCCATCTGATAAGCGCAAGTATACCTCCGAGAAAATTCTGCTGGGTCATACTAGCTTTGTTGGGCCACTTGCATGGATCCCACCAACTGATGAATACCCTGAAGGAAGACTTGTCTCTGGGAGTATGGATACTTTCGTTTTTGTTTGGAATCTGATGAATGGAGAGAATATTCAGACATTGAAGGGTCATCAGATGCAAGTCACTGGTGTTGCTATTGATAATGAGGACATAGTTTCATCTTCAGTTGATCAAACCCTGAAACGATGGAGAAACGGTCAGCTTGTTGAATCTTGGGATGCACACCAGTCGCCTATCCAGGCAGTGATAAGGCTGCCATCTGGTGAGCTCGTTTCAGGTTCAAGTGACGCCAGTCTCAAACTCTGGAAGGGTAAAACGAGTCTGCAAACTCTTTCAGGGCATACAGATACTGTTAGAGGGTTAGCTGTGATGCCTGATTTAGGATTTCTTTCCGCATCACATGATGGTTCAATCAGGTTATGGGCACTAAGTGGCGAAGTTCTTCTTGAGATGGTTGGCCACACCTCCCTTGTTTATTCAGTTGACGCACATTCGTCTGGCCTTATTGTTAGTGCCAGTGAAGATCGTCATGCCAAGATATGGAAAGATGGAGTCTGCGTTCAGAGTTTGGAACACCCAGGCTGTATCTGGGATGCCAAGTTTTTGGAAACCGGAGATATTGTGACAGCATGTTCAGATGGAGTGGTTCGTGTTTGGACAGTACGTCATGATGCGATTGCAGATCAGATGGAGATTGATGCCTATGATTCCCAAATTTCTCAATACAAACTAAGCAGGAAAAAGGTTGGCGGATTGAAACTTGACGAGCTGCCAGGGCTTGATTCTTTGACGTCACCAGGTACCAGTGATGGCCAGACAAAAGTAGTAAGGGAAGGAGACAATGGTGTTGCTTATGCATGGAATATGAAAGAACAGAGATGGGACAAAATTGGTGAGGTTGTTGATGGCCCAGATGGTGTGGCTGATCGTCCAATTCACGAGGGAGTTCAATATGATTTTGTTTTCGATGTTGACATTGGTGATGGGGAACCTATCCGGAAGCTACCTTACAATCGATCAGACAATCCTTATGATGCAGCTGACAAGTGGCTTCTAAAAGAGAATCTCCCCTTTGCATATCGGCAGCAAATTGTTGAATTTATATTACAAAATTCTGGGCAGAAAGACTTCAACTTTAATCCATCTTTTCGTGATCCCTTCACCGGCGCAAATGCTTATGTGCCTGGGCAAGCATCTCGTACAGCTGCAACTCCGGCAAAACCTTTATACAAGCACATTCCAAAAAGAGGTGTGCTAGTTTTCGATGCTGCTCAATATGACGGGATTCTGAAAAAGATGACAGAGTTCAATACTACTTTACGATCTGACGCAGTAAACAATGACAAGTCCCTGACCGAACTCGAAGTATCCAGAGTAGGCGCAATCGTTAATATACTGAAGGACACATCACACTACCACTCTACCAATTTTGCTGACATGGATATTGCTTTGCTTTTGAAAGTGCTACAAGCATGGCCACCGGCAATGATGTTTCCTGCTACTGATATAGTAAGGATGCTCGTTTTGCATCATCATGGAGCAAGTCTACTTATCAAGCATGTGGAAAACAACAATGACCTGCTTTTGGATTTGATAAAGAAAGTTACAGAAGATTCTGCTCTTCCTGCAAATCTTTTAACGACTGTTCGTGTCCTTGTTAATCTATTCAAGAATTCTTCATTTCATTACTGGCTACAGACGCATCATAGTCAAATTCTTGATGCCTTCTCAAACTGTTATTCATCCCCAAATAAGAATCTGCAGTTGGCTTATTCGACGTTGCTTCTCAATTATGCAGTGCTATTAATAGAAAAGAAGGATCAAGAAGGTCAAGCTCAAGTCCTTTCAGCAGCTTTGCAGGTAGCAGAAGAAGAAGCTGCAGATGTTGATTCCAAGTTCCGGAGTCTGGTAGCAATTGGCTCACTGATGCTTGAAGGTCTCGTGAAGAAGATAGCGATAGATTTTGATGTAGAGAGCATCGCAAAATCAGCGAAGGCGTCAAAGGAAGCCAAGATTGCAGAAGTTGGGGCCGATATCGATCTTGTAATTCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

760

Amino Acids

84.27

Weight (kDa)

5.31

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 2 - 177 9e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 7 - 139 1.1e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 11 - 178 2e-23 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 11 - 140 2.6e-11 WDR3 second beta-propeller domain
WD40 PF00400 12 - 47 5.7e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 12 - 156 1.1e-15 TEP-1 second beta-propeller
WDR55 PF24796 13 - 257 2.1e-08 WDR55
Beta-prop_CAF1B_HIR1 PF24105 51 - 136 2.3e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 85 - 154 2.5e-07 THOC3 beta-propeller domain
WD40_Gbeta PF25391 85 - 178 2e-06 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 85 - 147 3.4e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 85 - 165 7.5e-08 Prp19 WD40 domain
EIF3I PF24805 86 - 155 5.6e-06 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 108 - 243 2.6e-08 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 121 - 256 3.5e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 139 - 296 7.3e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 140 - 309 2.8e-14 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 145 - 221 2e-06 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 148 - 318 9.5e-21 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 149 - 257 3.5e-07 SCAP Beta-propeller
Beta-prop_THOC3 PF25174 164 - 218 7e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 164 - 220 7.2e-06 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 164 - 264 4.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 181 - 257 4.7e-08 WDR41
WD40 PF00400 181 - 216 5.1e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 206 - 307 1.7e-07 THOC3 beta-propeller domain
PFU PF09070 341 - 450 3.3e-38 PFU (PLAA family ubiquitin binding)
PUL PF08324 488 - 753 7e-63 PUL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1019
Acc36I ACCTGC 2 cut(s) 1812, 2074
Acc65I GGTACC 1 cut(s) 1025
AccB1I GGYRCC 1 cut(s) 1025
AccB7I CCANNNNNTGG 1 cut(s) 765
AccI GTMKAC 2 cut(s) 167, 1771
AccIII TCCGGA 2 cut(s) 1221, 2124
AciI CCGC 2 cut(s) 618, 975
AclWI GGATC 6 cut(s) 140, 153, 217, 230, 1373, 2052
AcoI YGGCCR 4 cut(s) 52, 682, 1036, 1700
AcsI RAATTY 6 cut(s) 180, 939, 1322, 1336, 1897, 1938
AcuI CTGAAG 3 cut(s) 267, 372, 1643
AcyI GRCGYC 3 cut(s) 513, 1016, 2222
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 4 cut(s) 29, 888, 1027, 1426
AfiI CCNNNNNNNGG 7 cut(s) 605, 765, 1388, 1472, 1810, 2124, 2131
AhdI GACNNNNNGTC 1 cut(s) 888
AjnI CCWGG 5 cut(s) 462, 802, 997, 1021, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 1973, 2005
AloI GAACNNNNNNTCC 2 cut(s) 768, 800
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 4 cut(s) 265, 524, 1056, 2168
AlwI GGATC 6 cut(s) 140, 153, 217, 230, 1373, 2052
AlwNI CAGNNNCTG 1 cut(s) 575
Aor13HI TCCGGA 2 cut(s) 1221, 2124
AoxI GGCC 8 cut(s) 52, 209, 682, 724, 1036, 1132, 1700, 2253
ApeKI GCWGC 8 cut(s) 478, 994, 1262, 1309, 1430, 1493, 2075, 2102
ApoI RAATTY 6 cut(s) 180, 939, 1322, 1336, 1897, 1938
AseI ATTAAT 1 cut(s) 2030
Asp700I GAANNNNTTC 2 cut(s) 666, 1352
Asp718I GGTACC 1 cut(s) 1025
AspLEI GCGC 3 cut(s) 162, 1394, 1607
AspS9I GGNCC 4 cut(s) 123, 209, 1133, 2253
AsuHPI GGTGA 5 cut(s) 500, 1011, 1130, 1217, 1378
AvaII GGWCC 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 652
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BalI TGGCCA 4 cut(s) 54, 684, 1038, 1702
BamHI GGATCC 2 cut(s) 145, 222
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 1 cut(s) 495
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BauI CACGAG 2 cut(s) 1163, 2165
BbsI GAAGAC 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 8 cut(s) 465, 981, 1274, 1321, 1417, 1480, 2087, 2089
BcgI CGANNNNNNTGC 4 cut(s) 435, 469, 1403, 1437
BciT130I CCWGG 5 cut(s) 464, 804, 999, 1023, 1410
BciVI GTATCC 2 cut(s) 268, 1603
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 4 cut(s) 265, 524, 1056, 2168
BfaI CTAG 2 cut(s) 198, 1481
BfmI CTRYAG 3 cut(s) 1919, 1985, 2103
BfuAI ACCTGC 2 cut(s) 1812, 2074
BfuI GTATCC 2 cut(s) 268, 1603
BisI GCNGC 8 cut(s) 479, 995, 1263, 1310, 1431, 1494, 2076, 2103
BlsI GCNGC 8 cut(s) 480, 996, 1264, 1311, 1432, 1495, 2077, 2104
Bme1390I CCNGG 5 cut(s) 464, 804, 999, 1023, 1410
Bme18I GGWCC 1 cut(s) 123
BmeRI GACNNNNNGTC 1 cut(s) 888
BmgT120I GGNCC 4 cut(s) 123, 209, 1133, 2253
BmiI GGNNCC 5 cut(s) 147, 224, 1027, 1215, 2254
BmrFI CCNGG 5 cut(s) 464, 804, 999, 1023, 1410
BoxI GACNNNNGTC 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 692, 2046
Bsa29I ATCGAT 3 cut(s) 15, 1240, 2262
BsaBI GATNNNNATC 1 cut(s) 2262
BsaHI GRCGYC 3 cut(s) 513, 1016, 2222
BsaI GGTCTC 1 cut(s) 2168
BsaJI CCNNGG 3 cut(s) 802, 998, 1409
BsaWI WCCGGW 3 cut(s) 836, 1221, 2124
BsaXI ACNNNNNCTCC 6 cut(s) 406, 436, 768, 798, 831, 861
Bsc4I CCNNNNNNNGG 7 cut(s) 605, 765, 1388, 1472, 1810, 2124, 2131
Bse118I RCCGGY 2 cut(s) 1388, 1705
Bse1I ACTGG 7 cut(s) 147, 355, 451, 516, 741, 1029, 1919
Bse3DI GCAATG 1 cut(s) 1716
Bse8I GATNNNNATC 1 cut(s) 2262
BseAI TCCGGA 2 cut(s) 1221, 2124
BseBI CCWGG 5 cut(s) 464, 804, 999, 1023, 1410
BseCI ATCGAT 3 cut(s) 15, 1240, 2262
BseDI CCNNGG 3 cut(s) 802, 998, 1409
BseGI GGATG 4 cut(s) 448, 823, 1746, 1967
BseJI GATNNNNATC 1 cut(s) 2262
BseLI CCNNNNNNNGG 7 cut(s) 605, 765, 1388, 1472, 1810, 2124, 2131
BseMI GCAATG 1 cut(s) 1716
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 7 cut(s) 147, 355, 451, 516, 741, 1029, 1919
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 8 cut(s) 465, 981, 1274, 1321, 1417, 1480, 2087, 2089
BseYI CCCAGC 1 cut(s) 187
BshFI GGCC 8 cut(s) 54, 211, 684, 726, 1038, 1134, 1702, 2255
BshNI GGYRCC 1 cut(s) 1025
BshVI ATCGAT 3 cut(s) 15, 1240, 2262
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 6 cut(s) 837, 1222, 1389, 1439, 1706, 2125
BslFI GGGAC 2 cut(s) 1121, 1558
BslI CCNNNNNNNGG 7 cut(s) 605, 765, 1388, 1472, 1810, 2124, 2131
BsmAI GTCTC 4 cut(s) 265, 524, 1056, 2168
BsmFI GGGAC 2 cut(s) 1121, 1558
BsnI GGCC 8 cut(s) 54, 211, 684, 726, 1038, 1134, 1702, 2255
Bso31I GGTCTC 1 cut(s) 2168
Bsp1286I GDGCHC 2 cut(s) 495, 652
Bsp13I TCCGGA 2 cut(s) 1221, 2124
BspACI CCGC 2 cut(s) 618, 975
BspANI GGCC 8 cut(s) 54, 211, 684, 726, 1038, 1134, 1702, 2255
BspCNI CTCAG 1 cut(s) 48
BspDI ATCGAT 3 cut(s) 15, 1240, 2262
BspEI TCCGGA 2 cut(s) 1221, 2124
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 5 cut(s) 147, 224, 1027, 1215, 2254
BspMAI CTGCAG 2 cut(s) 1989, 2107
BspMI ACCTGC 2 cut(s) 1812, 2074
BspPI GGATC 6 cut(s) 140, 153, 217, 230, 1373, 2052
BspQI GCTCTTC 1 cut(s) 1851
BspT107I GGYRCC 1 cut(s) 1025
BspTNI GGTCTC 1 cut(s) 2168
BsrDI GCAATG 1 cut(s) 1716
BsrFI RCCGGY 2 cut(s) 1388, 1705
BsrI ACTGG 7 cut(s) 147, 355, 451, 516, 741, 1029, 1919
BssAI RCCGGY 2 cut(s) 1388, 1705
BssECI CCNNGG 3 cut(s) 802, 998, 1409
BssNAI GTATAC 1 cut(s) 168
BssNI GRCGYC 3 cut(s) 513, 1016, 2222
BssSI CACGAG 2 cut(s) 1163, 2165
Bst1107I GTATAC 1 cut(s) 168
Bst2BI CACGAG 2 cut(s) 1163, 2165
Bst2UI CCWGG 5 cut(s) 464, 804, 999, 1023, 1410
Bst4CI ACNGT 5 cut(s) 422, 577, 886, 1870, 1961
Bst6I CTCTTC 1 cut(s) 1851
BstACI GRCGYC 3 cut(s) 513, 1016, 2222
BstC8I GCNNGC 2 cut(s) 44, 1416
BstDEI CTNAG 3 cut(s) 35, 653, 956
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 4 cut(s) 448, 823, 1746, 1967
BstHHI GCGC 3 cut(s) 162, 1394, 1607
BstMAI GTCTC 4 cut(s) 265, 524, 1056, 2168
BstMWI GCNNNNNNNGC 7 cut(s) 1000, 1439, 1750, 1850, 1924, 2213, 2240
BstNI CCWGG 5 cut(s) 464, 804, 999, 1023, 1410
BstNSI RCATGY 2 cut(s) 858, 1787
BstPAI GACNNNNGTC 1 cut(s) 516
BstSCI CCNGG 5 cut(s) 462, 802, 997, 1021, 1408
BstSFI CTRYAG 3 cut(s) 1919, 1985, 2103
BstSLI GKGCMC 1 cut(s) 652
BstV1I GCAGC 8 cut(s) 465, 981, 1274, 1321, 1417, 1480, 2087, 2089
BstV2I GAAGAC 1 cut(s) 259
BstX2I RGATCY 2 cut(s) 145, 222
BstXI CCANNNNNNTGG 2 cut(s) 219, 829
BstYI RGATCY 2 cut(s) 145, 222
BstZ17I GTATAC 1 cut(s) 168
Bsu15I ATCGAT 3 cut(s) 15, 1240, 2262
BsuI GTATCC 2 cut(s) 268, 1603
BsuRI GGCC 8 cut(s) 54, 211, 684, 726, 1038, 1134, 1702, 2255
BsuTUI ATCGAT 3 cut(s) 15, 1240, 2262
BtgZI GCGATG 1 cut(s) 2188
BtsCI GGATG 4 cut(s) 448, 823, 1746, 1967
BtsI GCAGTG 2 cut(s) 474, 2028
BtsIMutI CAGTG 7 cut(s) 140, 348, 474, 748, 1036, 2028, 2147
BveI ACCTGC 2 cut(s) 1812, 2074
Cac8I GCNNGC 2 cut(s) 44, 1416
CaiI CAGNNNCTG 1 cut(s) 575
CciI TCATGA 1 cut(s) 892
CfoI GCGC 3 cut(s) 162, 1394, 1607
Cfr10I RCCGGY 2 cut(s) 1388, 1705
Cfr13I GGNCC 4 cut(s) 123, 209, 1133, 2253
ClaI ATCGAT 3 cut(s) 15, 1240, 2262
CseI GACGC 5 cut(s) 521, 719, 1562, 1933, 2211
CsiI ACCWGGT 1 cut(s) 1021
Csp6I GTAC 4 cut(s) 28, 887, 1026, 1425
CviQI GTAC 4 cut(s) 28, 887, 1026, 1425
DdeI CTNAG 3 cut(s) 35, 653, 956
DraIII CACNNNGTG 1 cut(s) 656
DriI GACNNNNNGTC 1 cut(s) 888
EaeI YGGCCR 4 cut(s) 52, 682, 1036, 1700
Eam1104I CTCTTC 1 cut(s) 1851
Eam1105I GACNNNNNGTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 1851
EciI GGCGGA 1 cut(s) 990
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 2168
Eco32I GATATC 2 cut(s) 13, 2260
Eco47I GGWCC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 3 cut(s) 267, 372, 1643
EcoICRI GAGCTC 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoRI GAATTC 1 cut(s) 1897
EcoRII CCWGG 5 cut(s) 462, 802, 997, 1021, 1408
EcoRV GATATC 2 cut(s) 13, 2260
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 495
FalI AAGNNNNNCTT 4 cut(s) 240, 272, 654, 686
FaqI GGGAC 2 cut(s) 1121, 1558
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 2 cut(s) 167, 1771
Fnu4HI GCNGC 8 cut(s) 479, 995, 1263, 1310, 1431, 1494, 2076, 2103
FokI GGATG 4 cut(s) 455, 830, 1753, 1954
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 8 cut(s) 479, 995, 1263, 1310, 1431, 1494, 2076, 2103
FspBI CTAG 2 cut(s) 198, 1481
GlaI GCGC 3 cut(s) 161, 1393, 1606
GluI GCNGC 8 cut(s) 479, 995, 1263, 1310, 1431, 1494, 2076, 2103
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 8 cut(s) 54, 211, 684, 726, 1038, 1134, 1702, 2255
HapII CCGG 6 cut(s) 837, 1222, 1389, 1439, 1706, 2125
HgaI GACGC 5 cut(s) 521, 719, 1562, 1933, 2211
HhaI GCGC 3 cut(s) 162, 1394, 1607
Hin1I GRCGYC 3 cut(s) 513, 1016, 2222
Hin6I GCGC 3 cut(s) 160, 1392, 1605
HinP1I GCGC 3 cut(s) 160, 1392, 1605
HincII GTYRAC 2 cut(s) 709, 1198
HindII GTYRAC 2 cut(s) 709, 1198
HpaII CCGG 6 cut(s) 837, 1222, 1389, 1439, 1706, 2125
HphI GGTGA 5 cut(s) 500, 1011, 1130, 1217, 1378
Hpy166II GTNNAC 5 cut(s) 168, 709, 1198, 1561, 1772
Hpy8I GTNNAC 5 cut(s) 168, 709, 1198, 1561, 1772
Hpy99I CGWCG 1 cut(s) 2005
HpyCH4III ACNGT 5 cut(s) 422, 577, 886, 1870, 1961
HpyCH4IV ACGT 3 cut(s) 889, 1016, 2003
HpyF10VI GCNNNNNNNGC 7 cut(s) 1000, 1439, 1750, 1850, 1924, 2213, 2240
HpyF3I CTNAG 3 cut(s) 35, 653, 956
HpySE526I ACGT 3 cut(s) 889, 1016, 2003
Hsp92I GRCGYC 3 cut(s) 513, 1016, 2222
HspAI GCGC 3 cut(s) 160, 1392, 1605
Kpn2I TCCGGA 2 cut(s) 1221, 2124
KpnI GGTACC 1 cut(s) 1029
Ksp22I TGATCA 1 cut(s) 394
LguI GCTCTTC 1 cut(s) 1851
LmnI GCTCC 1 cut(s) 1763
Lsp1109I GCAGC 8 cut(s) 465, 981, 1274, 1321, 1417, 1480, 2087, 2089
MabI ACCWGGT 1 cut(s) 1021
MaeI CTAG 2 cut(s) 198, 1481
MaeII ACGT 3 cut(s) 889, 1016, 2003
MaeIII GTNAC 6 cut(s) 138, 346, 509, 847, 1017, 1828
MfeI CAATTG 1 cut(s) 2139
MflI RGATCY 2 cut(s) 145, 222
MhlI GDGCHC 2 cut(s) 495, 652
MlsI TGGCCA 4 cut(s) 54, 684, 1038, 1702
MluNI TGGCCA 4 cut(s) 54, 684, 1038, 1702
MlyI GAGTC 3 cut(s) 553, 786, 2137
MnlI CCTC 7 cut(s) 181, 364, 575, 700, 1114, 1159, 1466
Mox20I TGGCCA 4 cut(s) 54, 684, 1038, 1702
Mph1103I ATGCAT 1 cut(s) 1084
MroI TCCGGA 2 cut(s) 1221, 2124
MroXI GAANNNNTTC 2 cut(s) 666, 1352
MscI TGGCCA 4 cut(s) 54, 684, 1038, 1702
MseI TTAA 5 cut(s) 1362, 1614, 1862, 1884, 2030
MslI CAYNNNNRTG 3 cut(s) 60, 217, 1758
Msp20I TGGCCA 4 cut(s) 54, 684, 1038, 1702
MspA1I CMGCKG 2 cut(s) 1265, 1430
MspI CCGG 6 cut(s) 837, 1222, 1389, 1439, 1706, 2125
MspR9I CCNGG 5 cut(s) 464, 804, 999, 1023, 1410
MunI CAATTG 1 cut(s) 2139
MvaI CCWGG 5 cut(s) 464, 804, 999, 1023, 1410
MwoI GCNNNNNNNGC 7 cut(s) 1000, 1439, 1750, 1850, 1924, 2213, 2240
NlaIV GGNNCC 5 cut(s) 147, 224, 1027, 1215, 2254
NmuCI GTSAC 5 cut(s) 138, 346, 509, 847, 1017
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 2 cut(s) 858, 1787
PagI TCATGA 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 1851
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdmI GAANNNNTTC 2 cut(s) 666, 1352
PfeI GAWTC 9 cut(s) 295, 434, 932, 1007, 1288, 1510, 1838, 1981, 2114
PflMI CCANNNNNTGG 1 cut(s) 765
PkrI GCNGC 8 cut(s) 480, 996, 1264, 1311, 1432, 1495, 2077, 2104
PleI GAGTC 3 cut(s) 552, 785, 2136
PpsI GAGTC 3 cut(s) 552, 785, 2136
PshAI GACNNNNGTC 1 cut(s) 516
PshBI ATTAAT 1 cut(s) 2030
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 5 cut(s) 462, 802, 997, 1021, 1408
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 5 cut(s) 462, 802, 997, 1021, 1408
PspN4I GGNNCC 5 cut(s) 147, 224, 1027, 1215, 2254
PspPI GGNCC 4 cut(s) 123, 209, 1133, 2253
PstI CTGCAG 2 cut(s) 1989, 2107
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 2 cut(s) 145, 222
PvuII CAGCTG 2 cut(s) 1265, 1430
RsaI GTAC 4 cut(s) 29, 888, 1027, 1426
RsaNI GTAC 4 cut(s) 28, 887, 1026, 1425
RseI CAYNNNNRTG 3 cut(s) 60, 217, 1758
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 1851
SaqAI TTAA 5 cut(s) 1362, 1614, 1862, 1884, 2030
SatI GCNGC 8 cut(s) 479, 995, 1263, 1310, 1431, 1494, 2076, 2103
Sau96I GGNCC 4 cut(s) 123, 209, 1133, 2253
SchI GAGTC 3 cut(s) 553, 786, 2137
ScrFI CCNGG 5 cut(s) 464, 804, 999, 1023, 1410
SduI GDGCHC 2 cut(s) 495, 652
SexAI ACCWGGT 1 cut(s) 1021
SfcI CTRYAG 3 cut(s) 1919, 1985, 2103
SgrAI CRCCGGYG 1 cut(s) 1388
SinI GGWCC 1 cut(s) 123
SmiMI CAYNNNNRTG 3 cut(s) 60, 217, 1758
SmlI CTYRAG 2 cut(s) 671, 2061
SmoI CTYRAG 2 cut(s) 671, 2061
SsiI CCGC 2 cut(s) 618, 975
SspI AATATT 1 cut(s) 316
SspMI CTAG 2 cut(s) 198, 1481
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 5 cut(s) 462, 802, 997, 1021, 1408
TaaI ACNGT 5 cut(s) 422, 577, 886, 1870, 1961
TaiI ACGT 3 cut(s) 892, 1019, 2006
TaqI TCGA 7 cut(s) 15, 1191, 1240, 1488, 1587, 2000, 2262
TaqII GACCGA 1 cut(s) 1595
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 9 cut(s) 295, 434, 932, 1007, 1288, 1510, 1838, 1981, 2114
Tru1I TTAA 5 cut(s) 1362, 1614, 1862, 1884, 2030
Tru9I TTAA 5 cut(s) 1362, 1614, 1862, 1884, 2030
TscAI CASTG 7 cut(s) 147, 355, 474, 748, 1036, 2028, 2154
TseFI GTSAC 5 cut(s) 138, 346, 509, 847, 1017
TseI GCWGC 8 cut(s) 478, 994, 1262, 1309, 1430, 1493, 2075, 2102
Tsp45I GTSAC 5 cut(s) 138, 346, 509, 847, 1017
TspDTI ATGAA 9 cut(s) 38, 99, 252, 317, 372, 1106, 1893, 1898, 1956
TspRI CASTG 7 cut(s) 147, 355, 474, 748, 1036, 2028, 2154
Van91I CCANNNNNTGG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 123
VspI ATTAAT 1 cut(s) 2030
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 6 cut(s) 180, 939, 1322, 1336, 1897, 1938
XceI RCATGY 2 cut(s) 858, 1787
XcmI CCANNNNNNNNNTGG 1 cut(s) 810
XmiI GTMKAC 2 cut(s) 167, 1771
XmnI GAANNNNTTC 2 cut(s) 666, 1352
XspI CTAG 2 cut(s) 198, 1481
ZraI GACGTC 1 cut(s) 1017
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.