RLG00000014418

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009603
Physical Location & Seq
Forward (+)
52964880 .. 52977355
12476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014418

Sequence Viewer

Length: 2370 bp
ATGGCCATGGATATCGATTTCAAAGAGTACCAATTGCGCTGCGAGCTCCGCGGCCACGAAGACGACGTTCGTGGAATTTGCGTGTGCGGCAATGAGGGAATAGCTACTTCTTCACGTGATCGAACTGTTAGGTTATGGTCCTTGGACCCTTCCGACGAACGCCGGTATGCGCCGGCGAAGATTCTGCTGGGCCACACGAGTTTCGTGGGCCCAGTTGTGTGGATTGTGCCCAATGAGGAGTATCCAGAAGGCCGAATTGTGTCCGGTGGCATGGACACTATGGTGTTGGTGTGGGACTTGAAGACTGGGGAGAAGGTCCAAACTCTCAAGGGACATCAGTTGCAGGTCACCGGCATTGCTTTCGATAATGGAGACATTGTTTCATCTTCTGTGGACAATACTTTAAAAAGGTGGAGGAATGGCCAACTGGTAGAATCTTGGGAGGCTCATAAGGCAGCAATCCAATCAGTTATAAAGCTTCCTTCTGGCGAACTTGTCACTGGTTCAAGTGATACTACTTTAAAACTTTGGAGAGGAACTACATGCACTCACACGTTTGTTGGGCATACAGATACTGTTAGAGGCTTGGCAATGATGTCTGGATTGGGAGTTCTTTCAGCTTCACATGATGGTTCTATCAGGTTATGGGCTCTCACCGGTGAAGTTTTAATGGAGATGGTTGGTCATACTTCTATTGTATATTCAGTTGATTCGCATGTTTCTGGACTAATAGTCAGTAGTAGCGAAGATTGTTTTGCCAAGATATGGAAAGATGGAGTTTGTGTTCAGAGCATAGAACATCCTGGTTGTGTTTGGGATGCAAAGTTCTTGGAAAATGGGGATATTGTGACAGCATGTTCTGATGGAGTCGTACGTATCTGGACAGTAAACCAGGATAAAATTGCTGATGCCCTAGAACTTGAGTCATATTTCTCCCAACTGACTCAGCACAAAATTAGCAGGAAGAGAGTTGGAGGATTGAAATTGGAAGATTTACCTGGGCTAGAGGCTTTACAGATTCCAGGAACCAGTGATGGTCAGACTAAGATTGTCAGAGAGGGGGACAATGGTGTGGCATATGCATGGAATGTCAGAGAACAGAAGTGGGATAAAATTGGCGAAGTTGTTGATGGGCCAGATGATGGCATGAAGCGTCCTGTTCTTGATGGAAGTGAATATGATTATGTGTTTGATGTCGACATTGGAGATGGTGAGCCTATACGGAAGCTGCCTTACAATCGATCAGATAATCCATATGACACTGCTGACAAGTGGCTTCTGAAGGAGAATCTTCCTCTTTCCTACCGTGAACAGGTTGTAGCGTTCATTCTCCAAAATACTGGACTGAAAGATTTCACACTTGATCCAACATTTCGTGATCCCTACACTGGGGCCAGTGCTTACGTACCAGGACAATCTTCAAGCAAGTCTGGTTCTGCAAAACCTACTTTCAAACATATTCCTAAGAAAGGAATGCTTGTTTTTGACGTGGCTCAGTTTGATGGGATTCTCAAGAAGACTACAGAGTTCAATAATAATCTACTGTCTGACCAGGAAAAGAGCAACTTGTCATTAAATGAAGTTGAGGTTTCCAGGCTGGGTGCTATTGTTAAAGTACTGAAGGACACATCACATTATCACTCTAGTAAATTTGCCGAAGGTGACATTGCTTTGTTGATGAGACTGCTAAAATCATGGCCAGTTGCAATGTTATTTCCAGTTATTGATACTTTGAGGATGATTGTCCTACACCCTGATGGAGCACGTCTACTTCTCCAGCATGTTAGAGCTGAAAATGATTTACTATGGGAAATGATAAAAAATGTCGCAATAAATCACTCTGTTCCCGCAAACCTCTTAACAAGTGTTCGTGTTGTAACAAATCTTTTCAAGAACTCGAGCTTCCACAACTGGTTACAATTGCATCGCAGTGAGATTCTAGATGTGTATTCAAGCTGTTATTCATCTCCAAATAAGAATTTACAGTTATCATATGCAACATTGATACTCAATTATGCTGTTTTGTTGATTGATAACAAGGACCGGGAAGGTCAGTCACAGGTTCTTTCAGCTGCACTTCAGATTGCTGAGGAGGAAAATCTGGAAATTGATGCAAAGTTTCGGGCATTAGTTGCCATTGGATCACTGATGCTCGAAGGCTCCGTAAAACAGATAGCTATCGACTTTGATGTTGAGAATATTGCTAAAATAGCAAGAGCTTCTAAAGAGACAAAGATTGCTGAAGTTGGGGTTGACATTGAATGGAAGAATTTTAACTGTTTCGATGACTTTACAGTTGACACAGGGTTAGGAAGCGTTTATCTTCAGGTTGAAGATGTTGATTGGACTGCAGAATCCATGCTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

790

Amino Acids

87.72

Weight (kDa)

5.19

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 3 - 177 4.3e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 7 - 139 8e-18 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 12 - 156 6.2e-13 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 13 - 196 2.2e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 17 - 168 2.6e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 31 - 140 5.5e-09 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 74 - 256 3.9e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 83 - 178 1.5e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 84 - 178 1.1e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 85 - 312 1.7e-18 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 85 - 147 6.5e-06 WDHD1 first WD40 domain
WDR55 PF24796 85 - 256 3.8e-10 WDR55
Beta-prop_WDR3_2nd PF25172 119 - 222 7.7e-09 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 139 - 296 1.2e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 140 - 309 7.4e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 148 - 305 3.4e-20 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 149 - 297 2.1e-07 SCAP Beta-propeller
Beta-prop_TEP1_2nd PF25047 163 - 265 1.5e-06 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 187 - 313 7.7e-06 WDR36/Utp21 second beta-propeller domain
PFU PF09070 341 - 448 1.2e-38 PFU (PLAA family ubiquitin binding)
PUL PF08324 487 - 750 6.2e-59 PUL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 334
AccB7I CCANNNNNTGG 2 cut(s) 765, 1142
AccI GTMKAC 2 cut(s) 1197, 1768
AccII CGCG 1 cut(s) 51
AciI CCGC 4 cut(s) 49, 51, 87, 1848
AclWI GGATC 3 cut(s) 1358, 1373, 2149
AcoI YGGCCR 4 cut(s) 3, 52, 421, 1697
AcsI RAATTY 4 cut(s) 75, 1649, 1978, 2269
AcuI CTGAAG 5 cut(s) 1301, 1640, 2063, 2262, 2309
AcvI CACGTG 1 cut(s) 116
AfaI GTAC 4 cut(s) 29, 873, 1407, 1617
AfiI CCNNNNNNNGG 6 cut(s) 765, 1142, 1312, 1388, 1389, 1469
AflIII ACRYGT 1 cut(s) 552
AgeI ACCGGT 1 cut(s) 656
AhdI GACNNNNNGTC 1 cut(s) 731
AjiI CACGTC 2 cut(s) 1489, 1766
AjnI CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
AleI CACNNNNGTG 1 cut(s) 281
AloI GAACNNNNNNTCC 6 cut(s) 594, 626, 768, 800, 809, 841
Alw21I GWGCWC 2 cut(s) 48, 1765
Alw26I GTCTC 3 cut(s) 366, 1675, 2222
AlwI GGATC 3 cut(s) 1358, 1373, 2149
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 1897
AoxI GGCC 9 cut(s) 3, 52, 190, 208, 250, 421, 1133, 1392, 1697
ApaI GGGCCC 1 cut(s) 212
ApeKI GCWGC 4 cut(s) 39, 455, 1228, 2072
ApoI RAATTY 4 cut(s) 75, 1649, 1978, 2269
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 1352
AspLEI GCGC 2 cut(s) 39, 172
AspS9I GGNCC 9 cut(s) 138, 145, 190, 208, 209, 316, 1133, 1392, 2041
AsuC2I CCSGG 1 cut(s) 2045
AsuHPI GGTGA 5 cut(s) 340, 646, 671, 1223, 1673
AvaI CYCGRG 1 cut(s) 1897
AvaII GGWCC 4 cut(s) 138, 145, 316, 2041
BaeGI GKGCMC 2 cut(s) 212, 231
BalI TGGCCA 3 cut(s) 5, 423, 1699
BanII GRGCYC 3 cut(s) 48, 212, 652
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BauI CACGAG 1 cut(s) 196
BbrPI CACGTG 1 cut(s) 116
BbsI GAAGAC 3 cut(s) 66, 308, 1523
Bbv12I GWGCWC 2 cut(s) 48, 1765
BbvCI CCTCAGC 1 cut(s) 2088
BbvI GCAGC 4 cut(s) 26, 467, 1215, 2059
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 343, 377
BciT130I CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 2045
BcoDI GTCTC 3 cut(s) 366, 1675, 2222
BfaI CTAG 4 cut(s) 914, 1004, 1644, 1940
BfmI CTRYAG 2 cut(s) 1521, 2349
BfuAI ACCTGC 1 cut(s) 334
BfuI GTATCC 1 cut(s) 252
BisI GCNGC 6 cut(s) 40, 52, 88, 456, 1229, 2073
BlsI GCNGC 6 cut(s) 41, 53, 89, 457, 1230, 2074
BmcAI AGTACT 1 cut(s) 1617
Bme1390I CCNGG 8 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594, 2045
Bme18I GGWCC 4 cut(s) 138, 145, 316, 2041
BmeRI GACNNNNNGTC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 1897
BmgBI CACGTC 2 cut(s) 1489, 1766
BmgT120I GGNCC 9 cut(s) 138, 145, 190, 208, 209, 316, 1133, 1392, 2041
BmiI GGNNCC 5 cut(s) 147, 210, 1027, 1393, 2161
BmrFI CCNGG 8 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594, 2045
BmrI ACTGGG 3 cut(s) 206, 315, 1398
BmsI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
BmuI ACTGGG 3 cut(s) 206, 315, 1398
BpiI GAAGAC 3 cut(s) 66, 308, 1523
BpmI CTGGAG 1 cut(s) 1760
Bpu10I CCTNAGC 1 cut(s) 2088
BpuEI CTTGAG 3 cut(s) 311, 941, 1496
BpuMI CCSGG 1 cut(s) 2045
Bsa29I ATCGAT 2 cut(s) 15, 1240
BsaAI YACGTR 3 cut(s) 116, 875, 1405
BsaBI GATNNNNATC 1 cut(s) 2177
BsaJI CCNNGG 4 cut(s) 6, 49, 141, 998
BsaWI WCCGGW 2 cut(s) 263, 656
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 768, 798
Bsc4I CCNNNNNNNGG 6 cut(s) 765, 1142, 1312, 1388, 1389, 1469
Bse118I RCCGGY 4 cut(s) 162, 172, 350, 656
Bse3DI GCAATG 5 cut(s) 97, 354, 597, 1665, 1713
Bse8I GATNNNNATC 1 cut(s) 2177
BseBI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BseCI ATCGAT 2 cut(s) 15, 1240
BseDI CCNNGG 4 cut(s) 6, 49, 141, 998
BseGI GGATG 3 cut(s) 799, 823, 1743
BseJI GATNNNNATC 1 cut(s) 2177
BseLI CCNNNNNNNGG 6 cut(s) 765, 1142, 1312, 1388, 1389, 1469
BseMI GCAATG 5 cut(s) 97, 354, 597, 1665, 1713
BseMII CTCAG 3 cut(s) 959, 1508, 2079
BseRI GAGGAG 2 cut(s) 251, 2105
BseSI GKGCMC 2 cut(s) 212, 231
BseXI GCAGC 4 cut(s) 26, 467, 1215, 2059
BseYI CCCAGC 2 cut(s) 187, 1597
BsgI GTGCAG 1 cut(s) 2058
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 9 cut(s) 5, 54, 192, 210, 252, 423, 1135, 1394, 1699
BshTI ACCGGT 1 cut(s) 656
BshVI ATCGAT 2 cut(s) 15, 1240
BsiHKAI GWGCWC 2 cut(s) 48, 1765
BsiHKCI CYCGRG 1 cut(s) 1897
BsiSI CCGG 6 cut(s) 163, 173, 264, 351, 657, 2044
BsiWI CGTACG 1 cut(s) 871
BslFI GGGAC 3 cut(s) 308, 345, 1076
BslI CCNNNNNNNGG 6 cut(s) 765, 1142, 1312, 1388, 1389, 1469
BsmAI GTCTC 3 cut(s) 366, 1675, 2222
BsmFI GGGAC 3 cut(s) 308, 345, 1076
BsmI GAATGC 1 cut(s) 1479
BsnI GGCC 9 cut(s) 5, 54, 192, 210, 252, 423, 1135, 1394, 1699
BsoBI CYCGRG 1 cut(s) 1897
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 5 cut(s) 48, 212, 231, 652, 1765
Bsp143I GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 4 cut(s) 49, 51, 87, 1848
BspANI GGCC 9 cut(s) 5, 54, 192, 210, 252, 423, 1135, 1394, 1699
BspCNI CTCAG 3 cut(s) 958, 1507, 2080
BspDI ATCGAT 2 cut(s) 15, 1240
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 5 cut(s) 147, 210, 1027, 1393, 2161
BspMAI CTGCAG 1 cut(s) 2353
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 3 cut(s) 1358, 1373, 2149
BsrDI GCAATG 5 cut(s) 97, 354, 597, 1665, 1713
BsrFI RCCGGY 4 cut(s) 162, 172, 350, 656
BssAI RCCGGY 4 cut(s) 162, 172, 350, 656
BssECI CCNNGG 4 cut(s) 6, 49, 141, 998
BssMI GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
BssSI CACGAG 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 6, 141
Bst2BI CACGAG 1 cut(s) 196
Bst2UI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
Bst4CI ACNGT 8 cut(s) 127, 577, 886, 1307, 1545, 1986, 2279, 2296
Bst6I CTCTTC 1 cut(s) 959
BstAPI GCANNNNNTGC 1 cut(s) 2132
BstBAI YACGTR 3 cut(s) 116, 875, 1405
BstC8I GCNNGC 2 cut(s) 44, 174
BstDEI CTNAG 5 cut(s) 945, 1044, 1464, 1494, 2088
BstDSI CCRYGG 2 cut(s) 6, 49
BstEII GGTNACC 1 cut(s) 346
BstENI CCTNNNNNAGG 1 cut(s) 1467
BstF5I GGATG 3 cut(s) 799, 823, 1743
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 2 cut(s) 39, 172
BstKTI GATC 5 cut(s) 121, 1244, 1366, 1381, 2144
BstMAI GTCTC 3 cut(s) 366, 1675, 2222
BstMBI GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
BstMWI GCNNNNNNNGC 6 cut(s) 43, 48, 87, 452, 2132, 2210
BstNI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BstNSI RCATGY 4 cut(s) 546, 719, 858, 1784
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 8 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592, 2043
BstSFI CTRYAG 2 cut(s) 1521, 2349
BstSLI GKGCMC 2 cut(s) 212, 231
BstSNI TACGTA 2 cut(s) 875, 1405
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 4 cut(s) 26, 467, 1215, 2059
BstV2I GAAGAC 3 cut(s) 66, 308, 1523
BstXI CCANNNNNNTGG 2 cut(s) 219, 1340
Bsu15I ATCGAT 2 cut(s) 15, 1240
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 9 cut(s) 5, 54, 192, 210, 252, 423, 1135, 1394, 1699
BsuTUI ATCGAT 2 cut(s) 15, 1240
BtgI CCRYGG 2 cut(s) 6, 49
BtgZI GCGATG 1 cut(s) 1910
BtrI CACGTC 2 cut(s) 1489, 1766
BtsCI GGATG 3 cut(s) 799, 823, 1743
BtsI GCAGTG 2 cut(s) 1260, 1936
BtsIMutI CAGTG 7 cut(s) 498, 1036, 1260, 1386, 1402, 1936, 2144
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 2 cut(s) 44, 174
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 2 cut(s) 39, 172
Cfr10I RCCGGY 4 cut(s) 162, 172, 350, 656
Cfr13I GGNCC 9 cut(s) 138, 145, 190, 208, 209, 316, 1133, 1392, 2041
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 2 cut(s) 15, 1240
CseI GACGC 1 cut(s) 1142
Csp6I GTAC 4 cut(s) 28, 872, 1406, 1616
CspAI ACCGGT 1 cut(s) 656
CviQI GTAC 4 cut(s) 28, 872, 1406, 1616
DdeI CTNAG 5 cut(s) 945, 1044, 1464, 1494, 2088
DpnI GATC 5 cut(s) 120, 1243, 1365, 1380, 2143
DpnII GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
DraI TTTAAA 2 cut(s) 405, 522
DriI GACNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 4 cut(s) 3, 52, 421, 1697
Eam1104I CTCTTC 1 cut(s) 959
Eam1105I GACNNNNNGTC 1 cut(s) 731
EarI CTCTTC 1 cut(s) 959
Ecl136II GAGCTC 1 cut(s) 46
Eco105I TACGTA 2 cut(s) 875, 1405
Eco130I CCWWGG 2 cut(s) 6, 141
Eco24I GRGCYC 3 cut(s) 48, 212, 652
Eco32I GATATC 1 cut(s) 13
Eco47I GGWCC 4 cut(s) 138, 145, 316, 2041
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 5 cut(s) 1301, 1640, 2063, 2262, 2309
Eco72I CACGTG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 1897
Eco91I GGTNACC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 1467
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
EcoRV GATATC 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 6, 141
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 3 cut(s) 48, 212, 652
ErhI CCWWGG 2 cut(s) 6, 141
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 1550, 1582
FaqI GGGAC 3 cut(s) 308, 345, 1076
FauI CCCGC 1 cut(s) 1855
FauNDI CATATG 3 cut(s) 1078, 1255, 1993
FblI GTMKAC 2 cut(s) 1197, 1768
Fnu4HI GCNGC 6 cut(s) 40, 52, 88, 456, 1229, 2073
FokI GGATG 3 cut(s) 786, 830, 1750
FriOI GRGCYC 3 cut(s) 48, 212, 652
Fsp4HI GCNGC 6 cut(s) 40, 52, 88, 456, 1229, 2073
FspBI CTAG 4 cut(s) 914, 1004, 1644, 1940
GlaI GCGC 2 cut(s) 38, 171
GluI GCNGC 6 cut(s) 40, 52, 88, 456, 1229, 2073
GsaI CCCAGC 2 cut(s) 191, 1601
GsuI CTGGAG 1 cut(s) 1760
HaeIII GGCC 9 cut(s) 5, 54, 192, 210, 252, 423, 1135, 1394, 1699
HapII CCGG 6 cut(s) 163, 173, 264, 351, 657, 2044
HgaI GACGC 1 cut(s) 1142
HhaI GCGC 2 cut(s) 39, 172
Hin6I GCGC 2 cut(s) 37, 170
HinP1I GCGC 2 cut(s) 37, 170
HincII GTYRAC 3 cut(s) 1198, 2254, 2299
HindII GTYRAC 3 cut(s) 1198, 2254, 2299
HindIII AAGCTT 1 cut(s) 476
HpaII CCGG 6 cut(s) 163, 173, 264, 351, 657, 2044
HphI GGTGA 5 cut(s) 340, 646, 671, 1223, 1673
Hpy166II GTNNAC 7 cut(s) 394, 889, 1198, 1310, 1769, 2254, 2299
Hpy8I GTNNAC 7 cut(s) 394, 889, 1198, 1310, 1769, 2254, 2299
Hpy99I CGWCG 2 cut(s) 68, 158
HpyAV CCTTC 9 cut(s) 159, 242, 307, 492, 1276, 1615, 1652, 2042, 2150
HpyCH4III ACNGT 8 cut(s) 127, 577, 886, 1307, 1545, 1986, 2279, 2296
HpyCH4IV ACGT 7 cut(s) 66, 115, 554, 874, 1404, 1488, 1765
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 48, 87, 452, 2132, 2210
HpyF3I CTNAG 5 cut(s) 945, 1044, 1464, 1494, 2088
HpySE526I ACGT 7 cut(s) 66, 115, 554, 874, 1404, 1488, 1765
HspAI GCGC 2 cut(s) 37, 170
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
LmnI GCTCC 3 cut(s) 51, 1760, 2165
Lsp1109I GCAGC 4 cut(s) 26, 467, 1215, 2059
LweI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
MaeI CTAG 4 cut(s) 914, 1004, 1644, 1940
MaeII ACGT 7 cut(s) 66, 115, 554, 874, 1404, 1488, 1765
MaeIII GTNAC 7 cut(s) 346, 496, 847, 1661, 1876, 1914, 2055
MalI GATC 5 cut(s) 120, 1243, 1365, 1380, 2143
MboI GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
MfeI CAATTG 2 cut(s) 32, 1919
MhlI GDGCHC 5 cut(s) 48, 212, 231, 652, 1765
MlsI TGGCCA 3 cut(s) 5, 423, 1699
MluNI TGGCCA 3 cut(s) 5, 423, 1699
MlyI GAGTC 3 cut(s) 876, 932, 937
MmeI TCCRAC 3 cut(s) 177, 952, 1391
Mox20I TGGCCA 3 cut(s) 5, 423, 1699
Mph1103I ATGCAT 1 cut(s) 1084
MreI CGCCGGCG 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 1352
MscI TGGCCA 3 cut(s) 5, 423, 1699
MseI TTAA 7 cut(s) 404, 521, 668, 1574, 1611, 1859, 2274
MslI CAYNNNNRTG 4 cut(s) 281, 1081, 1755, 1929
Msp20I TGGCCA 3 cut(s) 5, 423, 1699
MspA1I CMGCKG 2 cut(s) 51, 2072
MspI CCGG 6 cut(s) 163, 173, 264, 351, 657, 2044
MspR9I CCNGG 8 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594, 2045
MunI CAATTG 2 cut(s) 32, 1919
Mva1269I GAATGC 1 cut(s) 1479
MvaI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 6 cut(s) 43, 48, 87, 452, 2132, 2210
NaeI GCCGGC 1 cut(s) 174
NciI CCSGG 1 cut(s) 2045
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 3 cut(s) 1078, 1255, 1993
NdeII GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
NgoMIV GCCGGC 1 cut(s) 172
NlaIV GGNNCC 5 cut(s) 147, 210, 1027, 1393, 2161
NmuCI GTSAC 5 cut(s) 346, 496, 847, 1661, 2055
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 4 cut(s) 546, 719, 858, 1784
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 1897
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 2160
PctI GAATGC 1 cut(s) 1479
PdiI GCCGGC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 1352
PfeI GAWTC 8 cut(s) 181, 434, 710, 1018, 1288, 1507, 1936, 2354
Pfl23II CGTACG 1 cut(s) 871
PflMI CCANNNNNTGG 2 cut(s) 765, 1142
PfoI TCCNGGA 1 cut(s) 1021
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 6 cut(s) 41, 53, 89, 457, 1230, 2074
PleI GAGTC 3 cut(s) 875, 931, 937
PmaCI CACGTG 1 cut(s) 116
PmlI CACGTG 1 cut(s) 116
PpsI GAGTC 3 cut(s) 875, 931, 937
Ppu21I YACGTR 3 cut(s) 116, 875, 1405
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
PspCI CACGTG 1 cut(s) 116
PspEI GGTNACC 1 cut(s) 346
PspFI CCCAGC 2 cut(s) 187, 1597
PspGI CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
PspLI CGTACG 1 cut(s) 871
PspN4I GGNNCC 5 cut(s) 147, 210, 1027, 1393, 2161
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 9 cut(s) 138, 145, 190, 208, 209, 316, 1133, 1392, 2041
PspXI VCTCGAGB 1 cut(s) 1897
PstI CTGCAG 1 cut(s) 2353
PstNI CAGNNNCTG 1 cut(s) 575
PvuII CAGCTG 1 cut(s) 2072
RsaI GTAC 4 cut(s) 29, 873, 1407, 1617
RsaNI GTAC 4 cut(s) 28, 872, 1406, 1616
RseI CAYNNNNRTG 4 cut(s) 281, 1081, 1755, 1929
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 1196
SaqAI TTAA 7 cut(s) 404, 521, 668, 1574, 1611, 1859, 2274
SatI GCNGC 6 cut(s) 40, 52, 88, 456, 1229, 2073
Sau3AI GATC 5 cut(s) 118, 1241, 1363, 1378, 2141
Sau96I GGNCC 9 cut(s) 138, 145, 190, 208, 209, 316, 1133, 1392, 2041
ScaI AGTACT 1 cut(s) 1617
SchI GAGTC 3 cut(s) 876, 932, 937
ScrFI CCNGG 8 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594, 2045
SduI GDGCHC 5 cut(s) 48, 212, 231, 652, 1765
SfaNI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
SfcI CTRYAG 2 cut(s) 1521, 2349
Sfr274I CTCGAG 1 cut(s) 1897
Sfr303I CCGCGG 1 cut(s) 52
SgrAI CRCCGGYG 2 cut(s) 172, 656
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 4 cut(s) 138, 145, 316, 2041
SlaI CTCGAG 1 cut(s) 1897
SmiMI CAYNNNNRTG 4 cut(s) 281, 1081, 1755, 1929
SmlI CTYRAG 4 cut(s) 326, 920, 1511, 1897
SmoI CTYRAG 4 cut(s) 326, 920, 1511, 1897
SnaBI TACGTA 2 cut(s) 875, 1405
SsiI CCGC 4 cut(s) 49, 51, 87, 1848
SspI AATATT 1 cut(s) 2200
SspMI CTAG 4 cut(s) 914, 1004, 1644, 1940
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 8 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592, 2043
StyI CCWWGG 2 cut(s) 6, 141
TaaI ACNGT 8 cut(s) 127, 577, 886, 1307, 1545, 1986, 2279, 2296
TaiI ACGT 7 cut(s) 69, 118, 557, 877, 1407, 1491, 1768
TaqI TCGA 9 cut(s) 15, 121, 363, 1197, 1240, 1898, 2154, 2181, 2283
TatI WGTACW 1 cut(s) 1615
TauI GCSGC 2 cut(s) 54, 90
TfiI GAWTC 8 cut(s) 181, 434, 710, 1018, 1288, 1507, 1936, 2354
Tru1I TTAA 7 cut(s) 404, 521, 668, 1574, 1611, 1859, 2274
Tru9I TTAA 7 cut(s) 404, 521, 668, 1574, 1611, 1859, 2274
TscAI CASTG 7 cut(s) 505, 1036, 1267, 1393, 1402, 1936, 2151
TseFI GTSAC 5 cut(s) 346, 496, 847, 1661, 2055
TseI GCWGC 4 cut(s) 39, 455, 1228, 2072
Tsp45I GTSAC 5 cut(s) 346, 496, 847, 1661, 2055
TspDTI ATGAA 5 cut(s) 372, 1163, 1315, 1593, 1953
TspGWI ACGGA 2 cut(s) 1237, 2152
TspRI CASTG 7 cut(s) 505, 1036, 1267, 1393, 1402, 1936, 2151
Van91I CCANNNNNTGG 2 cut(s) 765, 1142
VpaK11BI GGWCC 4 cut(s) 138, 145, 316, 2041
XagI CCTNNNNNAGG 1 cut(s) 1467
XapI RAATTY 4 cut(s) 75, 1649, 1978, 2269
XbaI TCTAGA 1 cut(s) 1939
XceI RCATGY 4 cut(s) 546, 719, 858, 1784
XhoI CTCGAG 1 cut(s) 1897
XmiI GTMKAC 2 cut(s) 1197, 1768
XmnI GAANNNNTTC 1 cut(s) 1352
XspI CTAG 4 cut(s) 914, 1004, 1644, 1940
ZrmI AGTACT 1 cut(s) 1617
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.