FvH4_2g08030

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009603
Physical Location & Seq
Reverse (-)
6915552 .. 6928672
13121 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08030.t1

Sequence Viewer

Length: 2283 bp
ATGGCGATGGACATTGATTTCAAAGACTACCAATTGCGCTGCGAGCTCCGCGGCCACGAACAAGACGTTCGTGGAATTTGCGTGTGCGGCAATGCCGGTATAGCCACTTCTTCGCGTGATCGGACTGTTAGGTTATGGTCGTTGGACGCTTCCGACGAACGCCGGTATGAGCCGGCGAAGATTATGCTGGGCCACACGAGCTTTGTGGGCCCGGTGGTGTGGATTGAGCCCAGTGAGGAGTATCCGGAAGGCCGGGTTGTGTCTGGTGGCCTCGACACTATGGTGTTGGTGTGGGACTTGAAGACTGGGGACATGGTCCAGACTCTCAAGGGCCATCAGTTGCAGGTCACCGGCGTCGCTTTTGATGATGGCGACATTGTTTCTTCTTCTTTGGACAATACTTTGAAAAGGTGGAGGAATGGCCAACTGGTGGAATCTTGGGAGGCTCATAAGTCAGCAATCCAAGCAGTTATAAAGCTTCCTTCCGGCGAGCTTGTCACTGGTTCAAGTGATACTACTTTAAAACTTTGGAAAGGAACTACCTGCACTCACACGTTTGTTGGGCACACAGATACGGTTAGAGGCTTGGCAATGATGTCTGGATTGGGTGTTCTGTCTGCTTCACATGATGGTTCTATCAGGTTATGGGCTCTAACTGGTGAAGTTTTAATGGAGATGGTTGGGCATACTTCTATTGTCTATTCAGTTGATTCGCATGCTTCTGGACTAATAGTCAGTAGTAGTGAAGATTGTTTTGCCAAGATATGGAAAGATGGAGTTTGCGTCCAGAGCATAGAACATCCTGGTTGTGTTTGGGATGCAAAGTTCTTGGACAATGGGGATATTGTGACAGCATGTTCTGACGGAGTCGCACGTATCTGGACAGTAAACCAGGATAAAATTGCTGATGCCTTAGATCTTGAGTCATATTTCTCCCAGCTGACTCAGCACAAAATTAGCAGGAAGAGGGTTGGAGGATTGAAATTGGAAGATTTACCTGGGCTAGAGGCTTTAAAGATTCCAGGAACCAGTGATGGCCAGACTAAGATTGTCAGAGAGGGGGACAATGGTGTGGCATATGCATGGAATGTCAGAGAACAGAAGTGGGATAAAATTGGGGAAGTTGTTGATGGGCCAGATGATGGCATGAAGCGCCCTGTTTTTGATGGAAGTGAATATGATTATGTGTTTGATGTCGACATTGGAGATGGCGAGCCTGTTAGGAAGCTGCCTTACAATCGATCAGATAATCCGTATGACACTGCTGACAAGTGGCTTCTGAAGGAGAATCTTCCTCTTTCCTACCGTGAACAGGTGGTAGCGTTCATCCTTCAAAATACTGGACAGAAAGATTTCACTCTTGATCCAACATTTCGTGACCCCTACACTGGGGCTAGTGCTTATGTGCCAGGACAATCTTCAAGCAAGTCTGGTTCTGCAAAACCTACTTTCAAACATATTCCGAAGAAAGGAATGCTTGTTTTCGACATGGCTCAGTTTGATGGGATTCTCAAGAAGATTACAGAGTTCAATAATAATCTACTCTCTGACCAGGAAAAGAGCAACTTGTCTTTGAATGAAGTTGAGGTTTCCAGGCTGGGTGCTATTGTTAAAGTACTGAAGGACACATCACATTATCATTCTAGTAAATTTGCCGAAGGTGACATTGCTTTGTTGATGAGATTGCTAAAATCATGGCCAGTTACAATGTTATTTCCAGTTATTGATACCTTGAGGATGACTGTCTTACACCCCGATGGAGCGAGTCTTCTTCAACAGCATGTTAGAGCGGAAAATGATTTACTATGGGAAATGATAAAAAATGTCGCGATGAATCATTCTGTTCCTGCAAACCTCTTAACAAGTGTTCGTGTTGTAACAAATCTTTTCAAGAACTCGAGCTTCCACAACTGGTTACAACTGCATCGCAGTGAGATTCTGGATGTGTATTCAAGCTGTTGTTCATCTCCAAATAAGAATTTACAGTTATCATACGCAACATTGATACTCAATTATGCTGTTTTGTTGATTGATAACAAGGACCGGGAAGGTCAGTCACAGGTTCTTTCAGCTGCACTTCAGATTGCCGAGGAGGAAAATCTGGAAATTGATGCAAAATTCCGGGCTCTGGTTGCCATTGGATCACTGATGCATGAAGGCTCCGTAAAACAGATAGCTATCGACTTTGATGTTGAGAATATTGCTAAAATAACAAAGGCTTCTAAAGAGACAAAGATTGCTGAAGTTGGGGTTGACATTGAAGTGTTAACAAAACAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

761

Amino Acids

84.19

Weight (kDa)

5.34

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 3 - 177 1.9e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 7 - 139 1.1e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 178 1.6e-20 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 12 - 156 2.6e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 14 - 197 8.1e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 15 - 222 4.1e-17 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 82 - 256 5e-12 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 84 - 180 1.6e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 85 - 311 3.3e-17 THOC3 beta-propeller domain
WDR55 PF24796 85 - 257 5.7e-10 WDR55
Beta-prop_WDR3_1st PF25173 139 - 296 1.4e-19 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 148 - 305 1.2e-19 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 149 - 260 1.6e-07 SCAP Beta-propeller
Beta-prop_TEP1_2nd PF25047 163 - 265 8.7e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 166 - 306 8.7e-10 Prp19 WD40 domain
PFU PF09070 341 - 450 1.4e-39 PFU (PLAA family ubiquitin binding)
PUL PF08324 487 - 750 1e-59 PUL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
Acc36I ACCTGC 2 cut(s) 334, 551
AccB7I CCANNNNNTGG 3 cut(s) 430, 765, 1142
AccBSI CCGCTC 1 cut(s) 1790
AccI GTMKAC 1 cut(s) 1197
AccII CGCG 3 cut(s) 51, 115, 1829
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 4 cut(s) 49, 51, 87, 1790
AclWI GGATC 2 cut(s) 1358, 2149
AcoI YGGCCR 4 cut(s) 52, 421, 1036, 1697
AcsI RAATTY 4 cut(s) 75, 1649, 1978, 2117
AcuI CTGAAG 4 cut(s) 1301, 1640, 2063, 2262
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 1 cut(s) 1617
AfiI CCNNNNNNNGG 8 cut(s) 430, 765, 1142, 1312, 1388, 1389, 1469, 2128
AflIII ACRYGT 1 cut(s) 552
AhdI GACNNNNNGTC 1 cut(s) 731
AjnI CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
AleI CACNNNNGTG 1 cut(s) 281
AloI GAACNNNNNNTCC 4 cut(s) 594, 626, 809, 841
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 2222
AlwI GGATC 2 cut(s) 1358, 2149
Ama87I CYCGRG 1 cut(s) 1897
Aor13HI TCCGGA 1 cut(s) 244
ApaI GGGCCC 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 39, 1228, 2072
ApoI RAATTY 4 cut(s) 75, 1649, 1978, 2117
Asp700I GAANNNNTTC 1 cut(s) 1352
AspLEI GCGC 2 cut(s) 39, 1155
AspS9I GGNCC 7 cut(s) 190, 208, 209, 316, 331, 1133, 2041
AsuC2I CCSGG 4 cut(s) 212, 254, 2045, 2123
AsuHPI GGTGA 3 cut(s) 340, 671, 1673
AvaI CYCGRG 1 cut(s) 1897
AvaII GGWCC 2 cut(s) 316, 2041
BaeGI GKGCMC 2 cut(s) 212, 567
BalI TGGCCA 3 cut(s) 423, 1038, 1699
BanII GRGCYC 5 cut(s) 48, 212, 231, 652, 2128
BarI GAAGNNNNNNTAC 4 cut(s) 91, 123, 1217, 1249
BauI CACGAG 1 cut(s) 196
BbsI GAAGAC 2 cut(s) 308, 1760
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 26, 1215, 2059
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 4 cut(s) 212, 254, 2045, 2123
BcoDI GTCTC 1 cut(s) 2222
BfaI CTAG 3 cut(s) 1004, 1395, 1644
BfoI RGCGCY 1 cut(s) 1156
BfuAI ACCTGC 2 cut(s) 334, 551
BfuI GTATCC 1 cut(s) 252
BglII AGATCT 1 cut(s) 916
BisI GCNGC 5 cut(s) 40, 52, 88, 1229, 2073
BlsI GCNGC 5 cut(s) 41, 53, 89, 1230, 2074
BmcAI AGTACT 1 cut(s) 1617
Bme18I GGWCC 2 cut(s) 316, 2041
BmeRI GACNNNNNGTC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 1897
BmgT120I GGNCC 7 cut(s) 190, 208, 209, 316, 331, 1133, 2041
BmiI GGNNCC 3 cut(s) 210, 1027, 2161
BmrI ACTGGG 3 cut(s) 225, 315, 1398
BmsI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
BmuI ACTGGG 3 cut(s) 225, 315, 1398
BpiI GAAGAC 2 cut(s) 308, 1760
BpuEI CTTGAG 4 cut(s) 311, 941, 1496, 1753
BpuMI CCSGG 4 cut(s) 212, 254, 2045, 2123
Bsa29I ATCGAT 1 cut(s) 1240
BsaAI YACGTR 1 cut(s) 875
BsaBI GATNNNNATC 1 cut(s) 2177
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 49, 998, 2088
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 8 cut(s) 430, 765, 1142, 1312, 1388, 1389, 1469, 2128
Bse118I RCCGGY 4 cut(s) 95, 162, 172, 350
Bse3DI GCAATG 3 cut(s) 97, 597, 1665
Bse8I GATNNNNATC 1 cut(s) 2177
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BseCI ATCGAT 1 cut(s) 1240
BseDI CCNNGG 3 cut(s) 49, 998, 2088
BseGI GGATG 5 cut(s) 799, 823, 1326, 1743, 1948
BseJI GATNNNNATC 1 cut(s) 2177
BseLI CCNNNNNNNGG 8 cut(s) 430, 765, 1142, 1312, 1388, 1389, 1469, 2128
BseMI GCAATG 3 cut(s) 97, 597, 1665
BseMII CTCAG 2 cut(s) 959, 1508
BseRI GAGGAG 2 cut(s) 251, 2105
BseSI GKGCMC 2 cut(s) 212, 567
BseXI GCAGC 3 cut(s) 26, 1215, 2059
BseYI CCCAGC 3 cut(s) 187, 936, 1597
BsgI GTGCAG 2 cut(s) 529, 2058
Bsh1236I CGCG 3 cut(s) 51, 115, 1829
BshVI ATCGAT 1 cut(s) 1240
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 1897
BslFI GGGAC 3 cut(s) 308, 323, 1076
BslI CCNNNNNNNGG 8 cut(s) 430, 765, 1142, 1312, 1388, 1389, 1469, 2128
BsmAI GTCTC 1 cut(s) 2222
BsmFI GGGAC 3 cut(s) 308, 323, 1076
BsmI GAATGC 1 cut(s) 1479
BsoBI CYCGRG 1 cut(s) 1897
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 6 cut(s) 48, 212, 231, 567, 652, 2128
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 5 cut(s) 118, 916, 1241, 1363, 2141
Bsp68I TCGCGA 1 cut(s) 1829
BspACI CCGC 4 cut(s) 49, 51, 87, 1790
BspCNI CTCAG 2 cut(s) 958, 1507
BspDI ATCGAT 1 cut(s) 1240
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 3 cut(s) 51, 115, 1829
BspLI GGNNCC 3 cut(s) 210, 1027, 2161
BspMI ACCTGC 2 cut(s) 334, 551
BspPI GGATC 2 cut(s) 1358, 2149
BsrBI CCGCTC 1 cut(s) 1790
BsrDI GCAATG 3 cut(s) 97, 597, 1665
BsrFI RCCGGY 4 cut(s) 95, 162, 172, 350
BssAI RCCGGY 4 cut(s) 95, 162, 172, 350
BssECI CCNNGG 3 cut(s) 49, 998, 2088
BssMI GATC 5 cut(s) 118, 916, 1241, 1363, 2141
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 196
Bst2BI CACGAG 1 cut(s) 196
Bst2UI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
Bst4CI ACNGT 6 cut(s) 127, 577, 886, 1307, 1744, 1986
Bst6I CTCTTC 1 cut(s) 959
BstACI GRCGYC 1 cut(s) 354
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 5 cut(s) 44, 174, 491, 717, 1214
BstDEI CTNAG 4 cut(s) 913, 945, 1044, 1494
BstDSI CCRYGG 1 cut(s) 49
BstEII GGTNACC 1 cut(s) 346
BstF5I GGATG 5 cut(s) 799, 823, 1326, 1743, 1948
BstFNI CGCG 3 cut(s) 51, 115, 1829
BstH2I RGCGCY 1 cut(s) 1156
BstHHI GCGC 2 cut(s) 39, 1155
BstKTI GATC 5 cut(s) 121, 919, 1244, 1366, 2144
BstMAI GTCTC 1 cut(s) 2222
BstMBI GATC 5 cut(s) 118, 916, 1241, 1363, 2141
BstNI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
BstNSI RCATGY 3 cut(s) 719, 858, 1784
BstPI GGTNACC 1 cut(s) 346
BstSLI GKGCMC 2 cut(s) 212, 567
BstUI CGCG 3 cut(s) 51, 115, 1829
BstV1I GCAGC 3 cut(s) 26, 1215, 2059
BstV2I GAAGAC 2 cut(s) 308, 1760
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
Bsu15I ATCGAT 1 cut(s) 1240
BsuI GTATCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 1240
BtgI CCRYGG 1 cut(s) 49
BtgZI GCGATG 3 cut(s) 20, 1844, 1910
BtsCI GGATG 5 cut(s) 799, 823, 1326, 1743, 1948
BtsI GCAGTG 2 cut(s) 1260, 1936
BtsIMutI CAGTG 7 cut(s) 238, 498, 1036, 1260, 1386, 1936, 2144
BtuMI TCGCGA 1 cut(s) 1829
BveI ACCTGC 2 cut(s) 334, 551
Cac8I GCNNGC 5 cut(s) 44, 174, 491, 717, 1214
CfoI GCGC 2 cut(s) 39, 1155
Cfr10I RCCGGY 4 cut(s) 95, 162, 172, 350
Cfr13I GGNCC 7 cut(s) 190, 208, 209, 316, 331, 1133, 2041
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 1240
CseI GACGC 3 cut(s) 155, 343, 772
Csp6I GTAC 1 cut(s) 1616
CviQI GTAC 1 cut(s) 1616
DdeI CTNAG 4 cut(s) 913, 945, 1044, 1494
DpnI GATC 5 cut(s) 120, 918, 1243, 1365, 2143
DpnII GATC 5 cut(s) 118, 916, 1241, 1363, 2141
DraI TTTAAA 2 cut(s) 522, 1014
DriI GACNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 4 cut(s) 52, 421, 1036, 1697
Eam1104I CTCTTC 1 cut(s) 959
Eam1105I GACNNNNNGTC 1 cut(s) 731
EarI CTCTTC 1 cut(s) 959
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 5 cut(s) 48, 212, 231, 652, 2128
Eco47I GGWCC 2 cut(s) 316, 2041
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 4 cut(s) 1301, 1640, 2063, 2262
Eco88I CYCGRG 1 cut(s) 1897
Eco91I GGTNACC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
EcoT22I ATGCAT 2 cut(s) 1084, 2154
EcoT38I GRGCYC 5 cut(s) 48, 212, 231, 652, 2128
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 1550, 1582
FaqI GGGAC 3 cut(s) 308, 323, 1076
FauNDI CATATG 1 cut(s) 1078
FblI GTMKAC 1 cut(s) 1197
Fnu4HI GCNGC 5 cut(s) 40, 52, 88, 1229, 2073
FokI GGATG 5 cut(s) 786, 830, 1313, 1750, 1955
FriOI GRGCYC 5 cut(s) 48, 212, 231, 652, 2128
Fsp4HI GCNGC 5 cut(s) 40, 52, 88, 1229, 2073
FspBI CTAG 3 cut(s) 1004, 1395, 1644
GlaI GCGC 2 cut(s) 38, 1154
GluI GCNGC 5 cut(s) 40, 52, 88, 1229, 2073
GsaI CCCAGC 3 cut(s) 191, 940, 1601
HaeII RGCGCY 1 cut(s) 1156
HgaI GACGC 3 cut(s) 155, 343, 772
HhaI GCGC 2 cut(s) 39, 1155
Hin1I GRCGYC 1 cut(s) 354
Hin6I GCGC 2 cut(s) 37, 1153
HinP1I GCGC 2 cut(s) 37, 1153
HincII GTYRAC 3 cut(s) 1198, 2254, 2268
HindII GTYRAC 3 cut(s) 1198, 2254, 2268
HindIII AAGCTT 1 cut(s) 476
HpaI GTTAAC 1 cut(s) 2268
HphI GGTGA 3 cut(s) 340, 671, 1673
Hpy166II GTNNAC 5 cut(s) 889, 1198, 1310, 2254, 2268
Hpy8I GTNNAC 5 cut(s) 889, 1198, 1310, 2254, 2268
Hpy99I CGWCG 2 cut(s) 158, 359
HpyAV CCTTC 8 cut(s) 242, 492, 1276, 1340, 1615, 1652, 2042, 2150
HpyCH4III ACNGT 6 cut(s) 127, 577, 886, 1307, 1744, 1986
HpyCH4IV ACGT 3 cut(s) 66, 554, 874
HpyF3I CTNAG 4 cut(s) 913, 945, 1044, 1494
HpySE526I ACGT 3 cut(s) 66, 554, 874
Hsp92I GRCGYC 1 cut(s) 354
HspAI GCGC 2 cut(s) 37, 1153
Kpn2I TCCGGA 1 cut(s) 244
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
KspAI GTTAAC 1 cut(s) 2268
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 5 cut(s) 118, 916, 1241, 1363, 2141
LmnI GCTCC 3 cut(s) 51, 1760, 2165
Lsp1109I GCAGC 3 cut(s) 26, 1215, 2059
LweI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
MaeI CTAG 3 cut(s) 1004, 1395, 1644
MaeII ACGT 3 cut(s) 66, 554, 874
MaeIII GTNAC 9 cut(s) 346, 496, 847, 1376, 1661, 1702, 1876, 1914, 2055
MalI GATC 5 cut(s) 120, 918, 1243, 1365, 2143
MbiI CCGCTC 1 cut(s) 1790
MboI GATC 5 cut(s) 118, 916, 1241, 1363, 2141
MfeI CAATTG 1 cut(s) 32
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 6 cut(s) 48, 212, 231, 567, 652, 2128
MlsI TGGCCA 3 cut(s) 423, 1038, 1699
MluNI TGGCCA 3 cut(s) 423, 1038, 1699
MlyI GAGTC 5 cut(s) 316, 876, 932, 937, 1774
MmeI TCCRAC 4 cut(s) 123, 177, 952, 1391
Mox20I TGGCCA 3 cut(s) 423, 1038, 1699
Mph1103I ATGCAT 2 cut(s) 1084, 2154
MroI TCCGGA 1 cut(s) 244
MroNI GCCGGC 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 1352
MscI TGGCCA 3 cut(s) 423, 1038, 1699
MseI TTAA 6 cut(s) 521, 668, 1013, 1611, 1859, 2267
MslI CAYNNNNRTG 5 cut(s) 281, 1081, 1755, 1929, 2261
Msp20I TGGCCA 3 cut(s) 423, 1038, 1699
MspA1I CMGCKG 3 cut(s) 51, 940, 2072
MunI CAATTG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 1479
MvaI CCWGG 7 cut(s) 804, 893, 999, 1023, 1410, 1553, 1594
MvnI CGCG 3 cut(s) 51, 115, 1829
NaeI GCCGGC 1 cut(s) 174
NciI CCSGG 4 cut(s) 212, 254, 2045, 2123
NdeI CATATG 1 cut(s) 1078
NdeII GATC 5 cut(s) 118, 916, 1241, 1363, 2141
NgoMIV GCCGGC 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 210, 1027, 2161
NmeAIII GCCGAG 1 cut(s) 2113
NmuCI GTSAC 6 cut(s) 346, 496, 847, 1376, 1661, 2055
NruI TCGCGA 1 cut(s) 1829
NsiI ATGCAT 2 cut(s) 1084, 2154
NspI RCATGY 3 cut(s) 719, 858, 1784
OliI CACNNNNGTG 1 cut(s) 281
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 1 cut(s) 1897
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 153
PctI GAATGC 1 cut(s) 1479
PdiI GCCGGC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 1352
PfeI GAWTC 7 cut(s) 434, 710, 1018, 1288, 1507, 1834, 1936
PflFI GACNNNGTC 2 cut(s) 314, 866
PflMI CCANNNNNTGG 3 cut(s) 430, 765, 1142
PfoI TCCNGGA 1 cut(s) 1021
PkrI GCNGC 5 cut(s) 41, 53, 89, 1230, 2074
PleI GAGTC 5 cut(s) 316, 875, 931, 937, 1773
PpsI GAGTC 5 cut(s) 316, 875, 931, 937, 1773
Ppu21I YACGTR 1 cut(s) 875
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
PspEI GGTNACC 1 cut(s) 346
PspFI CCCAGC 3 cut(s) 187, 936, 1597
PspGI CCWGG 7 cut(s) 802, 891, 997, 1021, 1408, 1551, 1592
PspN4I GGNNCC 3 cut(s) 210, 1027, 2161
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 7 cut(s) 190, 208, 209, 316, 331, 1133, 2041
PspXI VCTCGAGB 1 cut(s) 1897
PsuI RGATCY 1 cut(s) 916
PsyI GACNNNGTC 2 cut(s) 314, 866
PvuII CAGCTG 2 cut(s) 940, 2072
RruI TCGCGA 1 cut(s) 1829
RsaI GTAC 1 cut(s) 1617
RsaNI GTAC 1 cut(s) 1616
RseI CAYNNNNRTG 5 cut(s) 281, 1081, 1755, 1929, 2261
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 1196
SaqAI TTAA 6 cut(s) 521, 668, 1013, 1611, 1859, 2267
SatI GCNGC 5 cut(s) 40, 52, 88, 1229, 2073
Sau3AI GATC 5 cut(s) 118, 916, 1241, 1363, 2141
Sau96I GGNCC 7 cut(s) 190, 208, 209, 316, 331, 1133, 2041
ScaI AGTACT 1 cut(s) 1617
SchI GAGTC 5 cut(s) 316, 876, 932, 937, 1774
SduI GDGCHC 6 cut(s) 48, 212, 231, 567, 652, 2128
SfaNI GCATC 5 cut(s) 808, 898, 1933, 2101, 2139
Sfr274I CTCGAG 1 cut(s) 1897
Sfr303I CCGCGG 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 2 cut(s) 316, 2041
SlaI CTCGAG 1 cut(s) 1897
SmiMI CAYNNNNRTG 5 cut(s) 281, 1081, 1755, 1929, 2261
SmlI CTYRAG 5 cut(s) 326, 920, 1511, 1732, 1897
SmoI CTYRAG 5 cut(s) 326, 920, 1511, 1732, 1897
SphI GCATGC 1 cut(s) 719
SsiI CCGC 4 cut(s) 49, 51, 87, 1790
SspI AATATT 1 cut(s) 2200
SspMI CTAG 3 cut(s) 1004, 1395, 1644
SstI GAGCTC 1 cut(s) 48
TaaI ACNGT 6 cut(s) 127, 577, 886, 1307, 1744, 1986
TaiI ACGT 3 cut(s) 69, 557, 877
TaqI TCGA 6 cut(s) 273, 1197, 1240, 1485, 1898, 2181
TatI WGTACW 1 cut(s) 1615
TauI GCSGC 2 cut(s) 54, 90
TfiI GAWTC 7 cut(s) 434, 710, 1018, 1288, 1507, 1834, 1936
Tru1I TTAA 6 cut(s) 521, 668, 1013, 1611, 1859, 2267
Tru9I TTAA 6 cut(s) 521, 668, 1013, 1611, 1859, 2267
TscAI CASTG 7 cut(s) 238, 505, 1036, 1267, 1393, 1936, 2151
TseFI GTSAC 6 cut(s) 346, 496, 847, 1376, 1661, 2055
TseI GCWGC 3 cut(s) 39, 1228, 2072
Tsp45I GTSAC 6 cut(s) 346, 496, 847, 1376, 1661, 2055
TspDTI ATGAA 6 cut(s) 1163, 1315, 1593, 1847, 1953, 2169
TspGWI ACGGA 3 cut(s) 879, 1242, 2152
TspRI CASTG 7 cut(s) 238, 505, 1036, 1267, 1393, 1936, 2151
Tth111I GACNNNGTC 2 cut(s) 314, 866
Van91I CCANNNNNTGG 3 cut(s) 430, 765, 1142
VpaK11BI GGWCC 2 cut(s) 316, 2041
XapI RAATTY 4 cut(s) 75, 1649, 1978, 2117
XceI RCATGY 3 cut(s) 719, 858, 1784
XhoI CTCGAG 1 cut(s) 1897
XmiI GTMKAC 1 cut(s) 1197
XmnI GAANNNNTTC 1 cut(s) 1352
XspI CTAG 3 cut(s) 1004, 1395, 1644
ZrmI AGTACT 1 cut(s) 1617
Zsp2I ATGCAT 2 cut(s) 1084, 2154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.