Prupe.8G104600_v2.0.a1

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009603
Physical Location & Seq
Forward (+)
13547404 .. 13560247
12844 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G104600.2

Sequence Viewer

Length: 2277 bp
ATGGACATCGATTTCAAAGAGTACCAATTGCGCTGCCAACTCCGAGGCCACGAAGACGACGTTCGTGGAATTTGTGTTTGCGGAAATGCGGGTATAGCCACCTCGTCACGTGATCGAACTGTGAGGCTGTGGTCCTTGGACCCTTCCGATGAAAGGCGGTACTCGGAGTCGAAGATTCTGCTGGGGCACACCAGTTTTGTGGGCCCATTGGTGTGGATTTCACCCAATGAGGAATACCCAGATGGCGGAATTGTGTCCGGCGGCATGGACACGATGGTATTGGTGTGGGACTTGAAGACTGGGGAGAAGGTGCAAACTCTCAGGGGCCATCAGTTGCAGGTCACGGGCCTCGCTTTGGATAATGGAGACATTGTCTCATCTTCTGTGGACTGTACTTTAAGACGGTGGAGGAAGGGCCAGTCATTAGAATCATGGGAGGCTCATAAGGCACCGATCCAAGCAGTTGTAAAGCTTCCTTCGGGCGAGCTTGTCACGGGTTCAAGTGATACTACTTTAAAACTTTGGAGAGGAAGTACATGCACTCACACTTTTGTTGGGCATACAGATACTGTTAGAGGCCTGGCAGTGATGTCTGGATTGGGAATTCTTTCTGCTTCTCATGATGGTTCCATTAGGTTATGGGCTCTAAGCGGTGAAGTTTTAATGGAGATGGTTGGGCATACTTCAATTGTATATTCAGTTGATTCACATGTGTCTGGATTAATAGTCAGTAGCAGCGAAGATTGTTTTGCTAAGATATGGAAAGATGGAGTTTGCGTGCAGAGCATAGAGCATCCTGGCTGTGTTTGGGATGCAAAATTCTTAGAAAATGGGGATATTGCGACAGCATGTTCTGATGGAGTTGTACGTATCTGGACTGTAAATCAGGATAAAATTGCTGATGCGCTAGAAGTGGAGTTATATTTCTCCCAGATTGCTCAACACAAAATTAGCAGGAAGAGGGTTGGAGGTTTGAAATTGGAAGAATTACCTGGGCTAGAGGCCTTACAGATTCCAGGAACCAGTGATGGCCAGACAAAAGTTGTCAGAGAAGGGGACAATGGTGTGGCGTATGCATGGAATATAAGAGAACAGAAGTGGGATAAGATTGGCGAAGTTGTTGATGGGCCAGATGGTGGCATGAAGCGTCCTGTTCTTGATGGAAATGAATATGACCATGTATTTGATGTTGACATTGGAGATGGCGAACCTATTCGTAAGCTGCCTTATAATCGATCAGATAATCCATATGATGCTGCTGACAAGTGGCTTCTGAAGGAGAATCTTCCTCTTTCCTACCGGGAACAGGTTGTAGCTTTCATTCTCCAAAATACTGGACAGAAAGACTTCACACTTGATCCATCATTTCGTGATCCCTATACTGGGTCTAGTGCTTATGTACCCGGACAATCTTCAAACAAGTCTGCTTCTGCAAAACCTACCTTCAAACATGTTCCTAAGAAAGGAATGCTTGTTTTTGACGTGGCTCAGTTTGATGGAATCCTTAAGAAGATTACAGAATTCAATAATAATCTACTCTCTGACCAGGAAAAGAAGAACTTGTCTTTAAATGAAGTGGAGGTTTCCAGGCTGGTTGCTATTGTTAAAATATTGAAGGACACGTCACATTATCATTCTAGTAAATTTGCCGAAGTTGACATTGCTATGTTGCTGAGGTTGCTAAAATCATGGCCAGTTGCAATGTTATTTCCAGTTATTGATACCTTGAGGATGATTGTCCTGCACCCTGATGGGGCAACTGTACTTCTCCAGCATGCTGAAGCTCAGAATGATTTTCTATTGGAAATGATCAAAAGAGTCGCAACAAATCCTTCACTTCCTGCTAATCTCTTAACTAGCGTTCGTGTTGTGACCAATCTTTTCAAGAATTCGTGCTACTACAATTGGTTACAAAAGCATCGGAGTGAGATTCTAGATGTGTATTCAAGTTGTTCTTCATCTCCGAATAAGAATTTGCAGTTATCATACGCAACCTTGATTCTCAATTATGCTGTTTTGTTGATCGAAAAGAAGGATCAGGAAGGGCAATCGCAGGTTCTTTCAGCAGCACTTCAGATTGCAGAAGAGGAAAATCTAGAAGTTGATGCAAAATTTCGAGTTTTAGTTGCCATCGGATCACTGATGCTTGAGGGCCTTGTGAAAAAGATAGCTATCGACTTTGATGTTGAAAACATTGCTAAAATATCAAAGGCTTCGAAAGAAACCAAGATTGCTGAAGTTGGAGTGGACATTGAACTTTTAACAAGACAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

759

Amino Acids

83.96

Weight (kDa)

5.56

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc36I ACCTGC 2 cut(s) 328, 2044
AccB1I GGYRCC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 1136
AciI CCGC 6 cut(s) 81, 89, 157, 246, 261, 651
AclWI GGATC 5 cut(s) 448, 1352, 1367, 2043, 2143
AcoI YGGCCR 2 cut(s) 1030, 1691
AcsI RAATTY 8 cut(s) 69, 603, 818, 1520, 1643, 1888, 1972, 2111
AcuI CTGAAG 4 cut(s) 1295, 1800, 2057, 2256
AcvI CACGTG 1 cut(s) 110
AfaI GTAC 7 cut(s) 23, 161, 394, 535, 867, 1401, 1764
AfiI CCNNNNNNNGG 9 cut(s) 153, 245, 355, 1136, 1306, 1382, 1383, 1463, 1753
AflII CTTAAG 1 cut(s) 1505
AflIII ACRYGT 3 cut(s) 709, 1450, 1620
AjiI CACGTC 2 cut(s) 1483, 1623
AjnI CCWGG 6 cut(s) 579, 796, 991, 1015, 1545, 1586
AluBI AGCT 7 cut(s) 472, 487, 1222, 1316, 1784, 2171, 2274
AluI AGCT 7 cut(s) 472, 487, 1222, 1316, 1784, 2171, 2274
Alw26I GTCTC 2 cut(s) 360, 379
AlwI GGATC 5 cut(s) 448, 1352, 1367, 2043, 2143
AlwNI CAGNNNCTG 1 cut(s) 569
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 5 cut(s) 33, 735, 1222, 1256, 2066
ApoI RAATTY 8 cut(s) 69, 603, 818, 1520, 1643, 1888, 1972, 2111
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AseI ATTAAT 1 cut(s) 722
Asp700I GAANNNNTTC 3 cut(s) 607, 1212, 1346
AspLEI GCGC 2 cut(s) 33, 907
AspS9I GGNCC 9 cut(s) 132, 139, 202, 203, 325, 346, 415, 1127, 2152
AsuC2I CCSGG 2 cut(s) 1301, 1404
AsuHPI GGTGA 2 cut(s) 213, 665
AsuII TTCGAA 1 cut(s) 2216
AvaII GGWCC 2 cut(s) 132, 139
BaeGI GKGCMC 2 cut(s) 189, 206
BalI TGGCCA 2 cut(s) 1032, 1693
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 2 cut(s) 206, 646
BbrPI CACGTG 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 60, 302
BbvCI CCTCAGC 1 cut(s) 1673
BbvI GCAGC 5 cut(s) 20, 747, 1209, 1243, 2078
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 6 cut(s) 581, 798, 993, 1017, 1547, 1588
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 2 cut(s) 1301, 1404
BcoDI GTCTC 2 cut(s) 360, 379
BfaI CTAG 7 cut(s) 908, 998, 1389, 1638, 1857, 1934, 2096
BfrI CTTAAG 1 cut(s) 1505
BfuAI ACCTGC 2 cut(s) 328, 2044
BisI GCNGC 6 cut(s) 34, 262, 736, 1223, 1257, 2067
BlsI GCNGC 6 cut(s) 35, 263, 737, 1224, 1258, 2068
Bme1390I CCNGG 8 cut(s) 581, 798, 993, 1017, 1301, 1404, 1547, 1588
Bme18I GGWCC 2 cut(s) 132, 139
BmgBI CACGTC 2 cut(s) 1483, 1623
BmgT120I GGNCC 9 cut(s) 132, 139, 202, 203, 325, 346, 415, 1127, 2152
BmiI GGNNCC 6 cut(s) 141, 204, 326, 450, 628, 1021
BmrFI CCNGG 8 cut(s) 581, 798, 993, 1017, 1301, 1404, 1547, 1588
BmrI ACTGGG 2 cut(s) 309, 1392
BmsI GCATC 7 cut(s) 802, 802, 892, 1243, 1927, 2095, 2133
BmuI ACTGGG 2 cut(s) 309, 1392
BpiI GAAGAC 2 cut(s) 60, 302
BpmI CTGGAG 1 cut(s) 1754
Bpu10I CCTNAGC 1 cut(s) 1673
Bpu14I TTCGAA 1 cut(s) 2216
BpuEI CTTGAG 2 cut(s) 1747, 2168
BpuMI CCSGG 2 cut(s) 1301, 1404
Bsa29I ATCGAT 2 cut(s) 9, 1234
BsaAI YACGTR 2 cut(s) 110, 869
BsaBI GATNNNNATC 1 cut(s) 2171
BsaJI CCNNGG 3 cut(s) 43, 135, 992
BsaXI ACNNNNNCTCC 6 cut(s) 357, 387, 517, 547, 762, 792
Bsc4I CCNNNNNNNGG 9 cut(s) 153, 245, 355, 1136, 1306, 1382, 1383, 1463, 1753
Bse1I ACTGG 8 cut(s) 192, 304, 418, 1023, 1339, 1387, 1694, 1712
Bse3DI GCAATG 3 cut(s) 1659, 1707, 2193
Bse8I GATNNNNATC 1 cut(s) 2171
BseBI CCWGG 6 cut(s) 581, 798, 993, 1017, 1547, 1588
BseCI ATCGAT 2 cut(s) 9, 1234
BseDI CCNNGG 3 cut(s) 43, 135, 992
BseGI GGATG 3 cut(s) 793, 817, 1737
BseJI GATNNNNATC 1 cut(s) 2171
BseLI CCNNNNNNNGG 9 cut(s) 153, 245, 355, 1136, 1306, 1382, 1383, 1463, 1753
BseMI GCAATG 3 cut(s) 1659, 1707, 2193
BseMII CTCAG 4 cut(s) 334, 1502, 1664, 1799
BseNI ACTGG 8 cut(s) 192, 304, 418, 1023, 1339, 1387, 1694, 1712
BseSI GKGCMC 2 cut(s) 189, 206
BseXI GCAGC 5 cut(s) 20, 747, 1209, 1243, 2078
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 2 cut(s) 800, 1727
BshNI GGYRCC 1 cut(s) 448
BshVI ATCGAT 2 cut(s) 9, 1234
BsiSI CCGG 3 cut(s) 258, 1300, 1404
BslFI GGGAC 2 cut(s) 302, 1070
BslI CCNNNNNNNGG 9 cut(s) 153, 245, 355, 1136, 1306, 1382, 1383, 1463, 1753
BsmAI GTCTC 2 cut(s) 360, 379
BsmFI GGGAC 2 cut(s) 302, 1070
BsmI GAATGC 1 cut(s) 1473
Bsp119I TTCGAA 1 cut(s) 2216
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 3 cut(s) 189, 206, 646
Bsp143I GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
BspACI CCGC 6 cut(s) 81, 89, 157, 246, 261, 651
BspCNI CTCAG 4 cut(s) 333, 1501, 1665, 1798
BspDI ATCGAT 2 cut(s) 9, 1234
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 6 cut(s) 141, 204, 326, 450, 628, 1021
BspMI ACCTGC 2 cut(s) 328, 2044
BspPI GGATC 5 cut(s) 448, 1352, 1367, 2043, 2143
BspT104I TTCGAA 1 cut(s) 2216
BspT107I GGYRCC 1 cut(s) 448
BspTI CTTAAG 1 cut(s) 1505
BsrDI GCAATG 3 cut(s) 1659, 1707, 2193
BsrI ACTGG 8 cut(s) 192, 304, 418, 1023, 1339, 1387, 1694, 1712
BssECI CCNNGG 3 cut(s) 43, 135, 992
BssMI GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 6 cut(s) 581, 798, 993, 1017, 1547, 1588
Bst4CI ACNGT 6 cut(s) 121, 392, 405, 571, 880, 1762
Bst6I CTCTTC 2 cut(s) 953, 2079
BstAFI CTTAAG 1 cut(s) 1505
BstBAI YACGTR 2 cut(s) 110, 869
BstBI TTCGAA 1 cut(s) 2216
BstC8I GCNNGC 3 cut(s) 485, 779, 1776
BstDEI CTNAG 8 cut(s) 320, 647, 753, 823, 1458, 1488, 1673, 1785
BstENI CCTNNNNNAGG 1 cut(s) 1461
BstF5I GGATG 3 cut(s) 793, 817, 1737
BstHHI GCGC 2 cut(s) 33, 907
BstKTI GATC 9 cut(s) 115, 456, 1238, 1360, 1375, 1812, 2025, 2038, 2138
BstMAI GTCTC 2 cut(s) 360, 379
BstMBI GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
BstMWI GCNNNNNNNGC 4 cut(s) 95, 446, 783, 1678
BstNI CCWGG 6 cut(s) 581, 798, 993, 1017, 1547, 1588
BstNSI RCATGY 5 cut(s) 540, 713, 852, 1454, 1778
BstSCI CCNGG 8 cut(s) 579, 796, 991, 1015, 1299, 1402, 1545, 1586
BstSLI GKGCMC 2 cut(s) 189, 206
BstSNI TACGTA 1 cut(s) 869
BstV1I GCAGC 5 cut(s) 20, 747, 1209, 1243, 2078
BstV2I GAAGAC 2 cut(s) 60, 302
BstXI CCANNNNNNTGG 3 cut(s) 199, 213, 1334
Bsu15I ATCGAT 2 cut(s) 9, 1234
BsuTUI ATCGAT 2 cut(s) 9, 1234
BtrI CACGTC 2 cut(s) 1483, 1623
BtsCI GGATG 3 cut(s) 793, 817, 1737
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 3 cut(s) 591, 1030, 2138
BveI ACCTGC 2 cut(s) 328, 2044
Cac8I GCNNGC 3 cut(s) 485, 779, 1776
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 619
CfoI GCGC 2 cut(s) 33, 907
Cfr13I GGNCC 9 cut(s) 132, 139, 202, 203, 325, 346, 415, 1127, 2152
ClaI ATCGAT 2 cut(s) 9, 1234
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 7 cut(s) 22, 160, 393, 534, 866, 1400, 1763
CviQI GTAC 7 cut(s) 22, 160, 393, 534, 866, 1400, 1763
DdeI CTNAG 8 cut(s) 320, 647, 753, 823, 1458, 1488, 1673, 1785
DpnI GATC 9 cut(s) 114, 455, 1237, 1359, 1374, 1811, 2024, 2037, 2137
DpnII GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
DraI TTTAAA 2 cut(s) 516, 1569
EaeI YGGCCR 2 cut(s) 1030, 1691
Eam1104I CTCTTC 2 cut(s) 953, 2079
EarI CTCTTC 2 cut(s) 953, 2079
EciI GGCGGA 1 cut(s) 261
Eco105I TACGTA 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 2 cut(s) 579, 1004
Eco24I GRGCYC 2 cut(s) 206, 646
Eco47I GGWCC 2 cut(s) 132, 139
Eco57I CTGAAG 4 cut(s) 1295, 1800, 2057, 2256
Eco72I CACGTG 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 1461
EcoO109I RGGNCCY 1 cut(s) 2152
EcoRI GAATTC 3 cut(s) 603, 1520, 1888
EcoRII CCWGG 6 cut(s) 579, 796, 991, 1015, 1545, 1586
EcoT14I CCWWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 2 cut(s) 206, 646
ErhI CCWWGG 1 cut(s) 135
FalI AAGNNNNNCTT 6 cut(s) 1455, 1487, 1544, 1576, 1939, 1971
FaqI GGGAC 2 cut(s) 302, 1070
FauI CCCGC 1 cut(s) 82
FauNDI CATATG 1 cut(s) 1249
FbaI TGATCA 1 cut(s) 1809
Fnu4HI GCNGC 6 cut(s) 34, 262, 736, 1223, 1257, 2067
FokI GGATG 3 cut(s) 780, 824, 1744
FriOI GRGCYC 2 cut(s) 206, 646
Fsp4HI GCNGC 6 cut(s) 34, 262, 736, 1223, 1257, 2067
FspBI CTAG 7 cut(s) 908, 998, 1389, 1638, 1857, 1934, 2096
GlaI GCGC 2 cut(s) 32, 906
GluI GCNGC 6 cut(s) 34, 262, 736, 1223, 1257, 2067
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 1 cut(s) 1754
HapII CCGG 3 cut(s) 258, 1300, 1404
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 2 cut(s) 33, 907
Hin6I GCGC 2 cut(s) 31, 905
HinP1I GCGC 2 cut(s) 31, 905
HincII GTYRAC 2 cut(s) 1192, 1657
HindII GTYRAC 2 cut(s) 1192, 1657
HindIII AAGCTT 1 cut(s) 470
HpaII CCGG 3 cut(s) 258, 1300, 1404
HphI GGTGA 2 cut(s) 213, 665
Hpy166II GTNNAC 4 cut(s) 388, 1192, 1657, 2248
Hpy8I GTNNAC 4 cut(s) 388, 1192, 1657, 2248
Hpy99I CGWCG 1 cut(s) 62
HpyCH4III ACNGT 6 cut(s) 121, 392, 405, 571, 880, 1762
HpyCH4IV ACGT 5 cut(s) 60, 109, 868, 1482, 1622
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 446, 783, 1678
HpyF3I CTNAG 8 cut(s) 320, 647, 753, 823, 1458, 1488, 1673, 1785
HpySE526I ACGT 5 cut(s) 60, 109, 868, 1482, 1622
HspAI GCGC 2 cut(s) 31, 905
Ksp22I TGATCA 1 cut(s) 1809
Kzo9I GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
Lsp1109I GCAGC 5 cut(s) 20, 747, 1209, 1243, 2078
LweI GCATC 7 cut(s) 802, 802, 892, 1243, 1927, 2095, 2133
MaeI CTAG 7 cut(s) 908, 998, 1389, 1638, 1857, 1934, 2096
MaeII ACGT 5 cut(s) 60, 109, 868, 1482, 1622
MaeIII GTNAC 6 cut(s) 105, 340, 490, 1623, 1870, 1908
MalI GATC 9 cut(s) 114, 455, 1237, 1359, 1374, 1811, 2024, 2037, 2137
MboI GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
MfeI CAATTG 3 cut(s) 26, 687, 1903
MhlI GDGCHC 3 cut(s) 189, 206, 646
MlsI TGGCCA 2 cut(s) 1032, 1693
MluNI TGGCCA 2 cut(s) 1032, 1693
MlyI GAGTC 2 cut(s) 176, 1827
MmeI TCCRAC 2 cut(s) 946, 2221
Mox20I TGGCCA 2 cut(s) 1032, 1693
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 3 cut(s) 607, 1212, 1346
MscI TGGCCA 2 cut(s) 1032, 1693
MseI TTAA 9 cut(s) 398, 515, 662, 722, 1506, 1568, 1605, 1853, 2261
MslI CAYNNNNRTG 4 cut(s) 211, 1664, 1749, 1923
Msp20I TGGCCA 2 cut(s) 1032, 1693
MspCI CTTAAG 1 cut(s) 1505
MspI CCGG 3 cut(s) 258, 1300, 1404
MspR9I CCNGG 8 cut(s) 581, 798, 993, 1017, 1301, 1404, 1547, 1588
MunI CAATTG 3 cut(s) 26, 687, 1903
Mva1269I GAATGC 1 cut(s) 1473
MvaI CCWGG 6 cut(s) 581, 798, 993, 1017, 1547, 1588
MwoI GCNNNNNNNGC 4 cut(s) 95, 446, 783, 1678
NciI CCSGG 2 cut(s) 1301, 1404
NdeI CATATG 1 cut(s) 1249
NdeII GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
NlaIV GGNNCC 6 cut(s) 141, 204, 326, 450, 628, 1021
NmuCI GTSAC 5 cut(s) 105, 340, 490, 1623, 1870
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 5 cut(s) 540, 713, 852, 1454, 1778
NspV TTCGAA 1 cut(s) 2216
PaeI GCATGC 1 cut(s) 1778
PagI TCATGA 1 cut(s) 619
PceI AGGCCT 2 cut(s) 579, 1004
PciI ACATGT 2 cut(s) 709, 1450
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 1473
PdmI GAANNNNTTC 3 cut(s) 607, 1212, 1346
PfeI GAWTC 8 cut(s) 175, 428, 704, 1012, 1282, 1500, 1930, 1999
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 1136
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 6 cut(s) 35, 263, 737, 1224, 1258, 2068
PleI GAGTC 2 cut(s) 175, 1826
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
PpsI GAGTC 2 cut(s) 175, 1826
Ppu21I YACGTR 2 cut(s) 110, 869
PscI ACATGT 2 cut(s) 709, 1450
PshBI ATTAAT 1 cut(s) 722
PsiI TTATAA 1 cut(s) 1230
Psp6I CCWGG 6 cut(s) 579, 796, 991, 1015, 1545, 1586
PspCI CACGTG 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 6 cut(s) 579, 796, 991, 1015, 1545, 1586
PspN4I GGNNCC 6 cut(s) 141, 204, 326, 450, 628, 1021
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 9 cut(s) 132, 139, 202, 203, 325, 346, 415, 1127, 2152
PstNI CAGNNNCTG 1 cut(s) 569
PsyI GACNNNGTC 1 cut(s) 371
RsaI GTAC 7 cut(s) 23, 161, 394, 535, 867, 1401, 1764
RsaNI GTAC 7 cut(s) 22, 160, 393, 534, 866, 1400, 1763
RseI CAYNNNNRTG 4 cut(s) 211, 1664, 1749, 1923
SaqAI TTAA 9 cut(s) 398, 515, 662, 722, 1506, 1568, 1605, 1853, 2261
SatI GCNGC 6 cut(s) 34, 262, 736, 1223, 1257, 2067
Sau3AI GATC 9 cut(s) 112, 453, 1235, 1357, 1372, 1809, 2022, 2035, 2135
Sau96I GGNCC 9 cut(s) 132, 139, 202, 203, 325, 346, 415, 1127, 2152
SchI GAGTC 2 cut(s) 176, 1827
ScrFI CCNGG 8 cut(s) 581, 798, 993, 1017, 1301, 1404, 1547, 1588
SduI GDGCHC 3 cut(s) 189, 206, 646
SfaNI GCATC 7 cut(s) 802, 802, 892, 1243, 1927, 2095, 2133
SfuI TTCGAA 1 cut(s) 2216
SinI GGWCC 2 cut(s) 132, 139
SmiMI CAYNNNNRTG 4 cut(s) 211, 1664, 1749, 1923
SmlI CTYRAG 3 cut(s) 1505, 1726, 2147
SmoI CTYRAG 3 cut(s) 1505, 1726, 2147
SnaBI TACGTA 1 cut(s) 869
SphI GCATGC 1 cut(s) 1778
SseBI AGGCCT 2 cut(s) 579, 1004
SsiI CCGC 6 cut(s) 81, 89, 157, 246, 261, 651
SspI AATATT 1 cut(s) 1611
SspMI CTAG 7 cut(s) 908, 998, 1389, 1638, 1857, 1934, 2096
StuI AGGCCT 2 cut(s) 579, 1004
StyD4I CCNGG 8 cut(s) 579, 796, 991, 1015, 1299, 1402, 1545, 1586
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 6 cut(s) 121, 392, 405, 571, 880, 1762
TaiI ACGT 5 cut(s) 63, 112, 871, 1485, 1625
TaqI TCGA 8 cut(s) 9, 115, 170, 1234, 2025, 2116, 2175, 2216
TatI WGTACW 3 cut(s) 392, 533, 1762
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 8 cut(s) 175, 428, 704, 1012, 1282, 1500, 1930, 1999
Tru1I TTAA 9 cut(s) 398, 515, 662, 722, 1506, 1568, 1605, 1853, 2261
Tru9I TTAA 9 cut(s) 398, 515, 662, 722, 1506, 1568, 1605, 1853, 2261
TscAI CASTG 3 cut(s) 591, 1030, 2145
TseFI GTSAC 5 cut(s) 105, 340, 490, 1623, 1870
TseI GCWGC 5 cut(s) 33, 735, 1222, 1256, 2066
Tsp45I GTSAC 5 cut(s) 105, 340, 490, 1623, 1870
TspDTI ATGAA 6 cut(s) 165, 1157, 1182, 1309, 1587, 1947
TspRI CASTG 3 cut(s) 591, 1030, 2145
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 1136
Vha464I CTTAAG 1 cut(s) 1505
VpaK11BI GGWCC 2 cut(s) 132, 139
VspI ATTAAT 1 cut(s) 722
XagI CCTNNNNNAGG 1 cut(s) 1461
XapI RAATTY 8 cut(s) 69, 603, 818, 1520, 1643, 1888, 1972, 2111
XbaI TCTAGA 2 cut(s) 1933, 2095
XceI RCATGY 5 cut(s) 540, 713, 852, 1454, 1778
XmnI GAANNNNTTC 3 cut(s) 607, 1212, 1346
XspI CTAG 7 cut(s) 908, 998, 1389, 1638, 1857, 1934, 2096
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.