MD10G1082200.v1.1

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009603
Physical Location & Seq
Forward (+)
12099719 .. 12115487
15769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1082200.v1.1.491

Sequence Viewer

Length: 2271 bp
ATGGACATCGATTTCAAAGAATACCAATTGCGCTGCCAACTCCGCGGCCACGAAGACGACGTTCGTGGGATTTCTGTGTGTGGCAATGCGGGTATAGCCACCTCGTCGCGTGATCGAACTGTTAGGTTCTGGTCCTTGGACCCTTCTGATGAACGGAGGTACTCGGAGTCGAAGATTCTGCTCGGCCACACCAGTTTTGTGGGCCCAGTGGTGTGGATTTCACCCAATGAGCAATACCCTGATGGTGGAATTGTGTCCGGCGGGATGGATACAATGGTGCTGGTGTGGGACTTGAGGACTGGGGAGAAGGTGCAAACTCTTAAGGGCCATCAGTTGCAGGTCACAGGCATCGCTTTGGATAATGGGGACATTGTCTCATCTTCTGTTGACTCTACTTTAAGGCGATGGAGGAATGGGCAGTTGGTAGAGTCTTGGGAGGCTCATAAGGCAGCCATCCAAGCAGTTATAAAGCTTCCTTCGGGCGAGCTTGTCACAGGTTCAAGCGATACTACTTTAAAACTTTGGAGGGGAAGTACATGCACTCGTACTTTTGTTGGGCATTCAGACACTGTAAGAGGCTTGGCAGTGATGTCCGAATTGGGAATTCTTTCTGCTTCACATGATGGTTCCATCAGGTTATGGGCTCTAAGTGGTGAAGTTTTAATGGAGATGGTTGGTCATACTTCAATTGTCTATTCTGTTGATTCACATGTGTCTGGACTAATAGTCAGTAGCAGCGAAGATTGTTTTGCAAAGATATGGAAGGATGGAGTTTGCATGCAGAGCATAGAGCATCCTGGCTGTGTTTGGGATGCAAAATTCTTGGAAAATGGGGATATTGTGACAGCATGTTCTGATGGAGCTGTACGCGTCTGGACCATAAATCAGGATAAAATTGCAGATGCCCTAGAAGTGGAGTCATATTTCTCCCAACTTTCTCAGCACAAAATTAGCAGGAAGAGGGTCGGAGGATTGAAACTGGAAGAATTACCTGGACTAGAAGCCTTACAGATTCCAGGAACCAGTGATGGCCAGACAAAAGTTGTCAGAGAGGGGGACAATGGTGTGGCATATGTATGGAATATGAGAGAACAGAAGTGGGATAAGATTGGCGAGGTTGTCGATGGGCCAGATGACAGCATGAAGCGTCCTGTTCTTGATGGAAACGAATATGATTATGTATTTGATGTCGACATTGGAGATGGAGAGCCTATTCGTAAGCTGCCTTACAATCGATCAGATAATCCATATGATACTGCTGACAAGTGGCTTCTGAGGGAGAACCTTCCTCTTTCCTACCGAGAACAAGTTGTCGCTTTCATCCTCCAAAATACTGGACAAAATAATTTCGACCTTGATCCATCATTTCGTGATCCCTACACTGGGTCAAGTGCTTATGTACCCGGACAATCTTCAAACAAGTCTGCTTCTGCAAAACCTACTTTCAAGCATATTCCTAAGAAAGGAATGCTTGTTTTTGACGTCGCCCAGTTTGATGGGATCCTAAAGAAGATTACAGAGTTCAACAGTAATTTACTCTCTGACCAGGATAAGAAGAACTCATCTTTAAATGAGGTTGAGATTTCCAGGTTGGGTGCTATAGTTAAAATATTGAAGGACACATCACATTATCATTCTAGTAAATTTGCCGAAGTTGACATTGCTTTGTTGGTGAGATTGCTAAAATCATGGCCAGTTGCAATGTTATTTCCAGTTATTGATATTTTGAGGATGATTGTTCTGCACCCTGATGGGGCAACTCGGCTTCTCCAGCATCTTGAAGCTGAAAATGATTTACTAATGGAGTTGATCAGAAAAGTTGCAACAAATCCGCCACTTCCTGCTAATCTCTTAACAAGTGTTCGTGCAGTGACCAATCTTTTCAAGAATTCGTGCTACTACAATTGGTTACAAAAGCATCGGACTGAGATTCTAGATGTGTATTCAAGTTGTTATTCATCTCCAAATAAGAATTTGCAGTTATCATATGCAACCTTGATTCTCAATTATGCTGTTTTGTTAACCGAAAAGAAGGATCAAGAAGGTCAATCCCAGGTTCTTTCTTCAGCACTTCAGATTGCAGAAGAAGAAAATCTAGACGCTGATGCAAAATTTCGGGCTTTAGTTGCCATTGGATCACTGATGCTTGAGGGCTTTGTGAAAAAGATAGCTATTGACTTTGATGTTGAAAACATTGCTAAAATAGCAAAGGCTTCCAAAGAAACCAAGATTGCTGAAGTTGGAGCGGACATTGAACTTTTAACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

757

Amino Acids

83.97

Weight (kDa)

5.38

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 2 - 175 1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 5 - 137 1.6e-17 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 11 - 153 9.8e-13 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 13 - 138 1.3e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 15 - 162 9.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 30 - 116 5.4e-10 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 50 - 134 3.9e-06 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 62 - 254 1.8e-08 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 80 - 175 1.8e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 83 - 174 1.5e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 83 - 176 1e-14 WDR3 first beta-propeller domain
WDR55 PF24796 84 - 254 3.7e-11 WDR55
WD40_Gbeta PF25391 119 - 254 2.3e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 137 - 294 3.4e-20 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 138 - 307 3.2e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 146 - 304 5.6e-20 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 147 - 296 2.3e-08 SCAP Beta-propeller
Beta-prop_TEP1_2nd PF25047 161 - 263 8.7e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 185 - 310 4.4e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 202 - 305 1.2e-06 THOC3 beta-propeller domain
PFU PF09070 339 - 448 1.5e-39 PFU (PLAA family ubiquitin binding)
PUL PF08324 485 - 750 4.4e-67 PUL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AatII GACGTC 1 cut(s) 1485
Acc36I ACCTGC 1 cut(s) 328
AccBSI CCGCTC 1 cut(s) 2246
AccI GTMKAC 1 cut(s) 1191
AccII CGCG 3 cut(s) 45, 109, 870
AciI CCGC 6 cut(s) 43, 45, 89, 261, 1832, 2246
AclWI GGATC 6 cut(s) 1352, 1367, 1495, 1508, 2043, 2143
AcoI YGGCCR 4 cut(s) 46, 184, 1030, 1691
AcsI RAATTY 6 cut(s) 603, 818, 1643, 1888, 1972, 2111
AcuI CTGAAG 3 cut(s) 2049, 2057, 2256
AcyI GRCGYC 1 cut(s) 1482
AfaI GTAC 5 cut(s) 161, 535, 547, 867, 1401
AfiI CCNNNNNNNGG 6 cut(s) 245, 913, 1382, 1383, 1463, 1753
AflII CTTAAG 1 cut(s) 320
AflIII ACRYGT 2 cut(s) 709, 868
AhdI GACNNNNNGTC 1 cut(s) 725
AjnI CCWGG 6 cut(s) 796, 991, 1015, 1545, 1586, 2052
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AluBI AGCT 6 cut(s) 472, 487, 863, 1222, 1784, 2171
AluI AGCT 6 cut(s) 472, 487, 863, 1222, 1784, 2171
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 6 cut(s) 1352, 1367, 1495, 1508, 2043, 2143
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 7 cut(s) 46, 184, 202, 325, 1030, 1127, 1691
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 4 cut(s) 33, 449, 735, 1222
ApoI RAATTY 6 cut(s) 603, 818, 1643, 1888, 1972, 2111
Asp700I GAANNNNTTC 1 cut(s) 607
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 7 cut(s) 132, 139, 202, 203, 325, 876, 1127
AsuC2I CCSGG 1 cut(s) 1404
AsuHPI GGTGA 3 cut(s) 213, 665, 1684
AvaII GGWCC 3 cut(s) 132, 139, 876
BaeGI GKGCMC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BalI TGGCCA 2 cut(s) 1032, 1693
BamHI GGATCC 1 cut(s) 1500
BanII GRGCYC 2 cut(s) 206, 646
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 4 cut(s) 20, 461, 747, 1209
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 6 cut(s) 798, 993, 1017, 1547, 1588, 2054
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 1 cut(s) 1404
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 5 cut(s) 908, 998, 1638, 1934, 2096
BfmI CTRYAG 1 cut(s) 1599
BfrI CTTAAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 328
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 5 cut(s) 34, 46, 450, 736, 1223
BlsI GCNGC 5 cut(s) 35, 47, 451, 737, 1224
Bme1390I CCNGG 7 cut(s) 798, 993, 1017, 1404, 1547, 1588, 2054
Bme18I GGWCC 3 cut(s) 132, 139, 876
BmeRI GACNNNNNGTC 1 cut(s) 725
BmgT120I GGNCC 7 cut(s) 132, 139, 202, 203, 325, 876, 1127
BmiI GGNNCC 5 cut(s) 141, 204, 628, 1021, 1502
BmrFI CCNGG 7 cut(s) 798, 993, 1017, 1404, 1547, 1588, 2054
BmrI ACTGGG 4 cut(s) 200, 309, 1392, 1483
BmsI GCATC 8 cut(s) 357, 802, 802, 892, 1783, 1927, 2095, 2133
BmuI ACTGGG 4 cut(s) 200, 309, 1392, 1483
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 1754
BpuEI CTTGAG 2 cut(s) 313, 2168
BpuMI CCSGG 1 cut(s) 1404
Bsa29I ATCGAT 2 cut(s) 9, 1234
BsaHI GRCGYC 1 cut(s) 1482
BsaJI CCNNGG 3 cut(s) 43, 135, 2052
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 6 cut(s) 245, 913, 1382, 1383, 1463, 1753
Bse3DI GCAATG 4 cut(s) 91, 1659, 1707, 2193
BseBI CCWGG 6 cut(s) 798, 993, 1017, 1547, 1588, 2054
BseCI ATCGAT 2 cut(s) 9, 1234
BseDI CCNNGG 3 cut(s) 43, 135, 2052
BseGI GGATG 7 cut(s) 270, 453, 772, 793, 817, 1320, 1737
BseLI CCNNNNNNNGG 6 cut(s) 245, 913, 1382, 1383, 1463, 1753
BseMI GCAATG 4 cut(s) 91, 1659, 1707, 2193
BseMII CTCAG 3 cut(s) 953, 1265, 1917
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 4 cut(s) 20, 461, 747, 1209
BsgI GTGCAG 2 cut(s) 1727, 1887
Bsh1236I CGCG 3 cut(s) 45, 109, 870
BshFI GGCC 7 cut(s) 48, 186, 204, 327, 1032, 1129, 1693
BshVI ATCGAT 2 cut(s) 9, 1234
BsiSI CCGG 2 cut(s) 258, 1404
BslFI GGGAC 3 cut(s) 302, 380, 1070
BslI CCNNNNNNNGG 6 cut(s) 245, 913, 1382, 1383, 1463, 1753
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 3 cut(s) 302, 380, 1070
BsmI GAATGC 2 cut(s) 559, 1473
BsnI GGCC 7 cut(s) 48, 186, 204, 327, 1032, 1129, 1693
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 206, 646
Bsp143I GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
BspACI CCGC 6 cut(s) 43, 45, 89, 261, 1832, 2246
BspANI GGCC 7 cut(s) 48, 186, 204, 327, 1032, 1129, 1693
BspCNI CTCAG 3 cut(s) 952, 1266, 1918
BspDI ATCGAT 2 cut(s) 9, 1234
BspFNI CGCG 3 cut(s) 45, 109, 870
BspLI GGNNCC 5 cut(s) 141, 204, 628, 1021, 1502
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 6 cut(s) 1352, 1367, 1495, 1508, 2043, 2143
BspTI CTTAAG 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 2246
BsrDI GCAATG 4 cut(s) 91, 1659, 1707, 2193
BssECI CCNNGG 3 cut(s) 43, 135, 2052
BssMI GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
BssNI GRCGYC 1 cut(s) 1482
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 6 cut(s) 798, 993, 1017, 1547, 1588, 2054
Bst4CI ACNGT 3 cut(s) 121, 571, 1529
Bst6I CTCTTC 1 cut(s) 953
BstACI GRCGYC 1 cut(s) 1482
BstAFI CTTAAG 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 485, 779
BstDEI CTNAG 5 cut(s) 647, 939, 1274, 1458, 1926
BstDSI CCRYGG 1 cut(s) 43
BstENI CCTNNNNNAGG 1 cut(s) 1461
BstF5I GGATG 7 cut(s) 270, 453, 772, 793, 817, 1320, 1737
BstFNI CGCG 3 cut(s) 45, 109, 870
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 8 cut(s) 115, 1238, 1360, 1375, 1503, 1812, 2038, 2138
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
BstMWI GCNNNNNNNGC 8 cut(s) 42, 95, 446, 458, 783, 1771, 2126, 2204
BstNI CCWGG 6 cut(s) 798, 993, 1017, 1547, 1588, 2054
BstNSI RCATGY 4 cut(s) 540, 713, 781, 852
BstSCI CCNGG 7 cut(s) 796, 991, 1015, 1402, 1545, 1586, 2052
BstSFI CTRYAG 1 cut(s) 1599
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 3 cut(s) 45, 109, 870
BstV1I GCAGC 4 cut(s) 20, 461, 747, 1209
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 1500
BstXI CCANNNNNNTGG 4 cut(s) 199, 213, 1334, 1496
BstYI RGATCY 1 cut(s) 1500
Bsu15I ATCGAT 2 cut(s) 9, 1234
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 7 cut(s) 48, 186, 204, 327, 1032, 1129, 1693
BsuTUI ATCGAT 2 cut(s) 9, 1234
BtgI CCRYGG 1 cut(s) 43
BtgZI GCGATG 2 cut(s) 334, 418
BtsCI GGATG 7 cut(s) 270, 453, 772, 793, 817, 1320, 1737
BtsI GCAGTG 2 cut(s) 591, 1875
BtsIMutI CAGTG 7 cut(s) 213, 567, 591, 1030, 1380, 1875, 2138
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 485, 779
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 7 cut(s) 132, 139, 202, 203, 325, 876, 1127
Cfr42I CCGCGG 1 cut(s) 46
ClaI ATCGAT 2 cut(s) 9, 1234
CseI GACGC 3 cut(s) 859, 1136, 2108
Csp6I GTAC 5 cut(s) 160, 534, 546, 866, 1400
CviAII CATG 7 cut(s) 537, 620, 710, 778, 849, 1141, 1689
CviQI GTAC 5 cut(s) 160, 534, 546, 866, 1400
DdeI CTNAG 5 cut(s) 647, 939, 1274, 1458, 1926
DpnI GATC 8 cut(s) 114, 1237, 1359, 1374, 1502, 1811, 2037, 2137
DpnII GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
DraI TTTAAA 2 cut(s) 516, 1569
DriI GACNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 4 cut(s) 46, 184, 1030, 1691
Eam1104I CTCTTC 1 cut(s) 953
Eam1105I GACNNNNNGTC 1 cut(s) 725
EarI CTCTTC 1 cut(s) 953
EciI GGCGGA 1 cut(s) 1821
Eco130I CCWWGG 1 cut(s) 135
Eco24I GRGCYC 2 cut(s) 206, 646
Eco47I GGWCC 3 cut(s) 132, 139, 876
Eco57I CTGAAG 3 cut(s) 2049, 2057, 2256
EcoNI CCTNNNNNAGG 1 cut(s) 1461
EcoRI GAATTC 2 cut(s) 603, 1888
EcoRII CCWGG 6 cut(s) 796, 991, 1015, 1545, 1586, 2052
EcoT14I CCWWGG 1 cut(s) 135
EcoT38I GRGCYC 2 cut(s) 206, 646
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 7 cut(s) 540, 623, 713, 781, 852, 1144, 1692
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FaqI GGGAC 3 cut(s) 302, 380, 1070
FatI CATG 7 cut(s) 536, 619, 709, 777, 848, 1140, 1688
FauI CCCGC 2 cut(s) 82, 254
FauNDI CATATG 3 cut(s) 1072, 1249, 1987
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 1191
Fnu4HI GCNGC 5 cut(s) 34, 46, 450, 736, 1223
FokI GGATG 7 cut(s) 277, 440, 779, 780, 824, 1307, 1744
FriOI GRGCYC 2 cut(s) 206, 646
Fsp4HI GCNGC 5 cut(s) 34, 46, 450, 736, 1223
FspBI CTAG 5 cut(s) 908, 998, 1638, 1934, 2096
GlaI GCGC 1 cut(s) 32
GluI GCNGC 5 cut(s) 34, 46, 450, 736, 1223
GsuI CTGGAG 1 cut(s) 1754
HaeIII GGCC 7 cut(s) 48, 186, 204, 327, 1032, 1129, 1693
HapII CCGG 2 cut(s) 258, 1404
HgaI GACGC 3 cut(s) 859, 1136, 2108
HhaI GCGC 1 cut(s) 33
Hin1I GRCGYC 1 cut(s) 1482
Hin1II CATG 7 cut(s) 540, 623, 713, 781, 852, 1144, 1692
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 4 cut(s) 388, 1192, 1657, 2022
HindII GTYRAC 4 cut(s) 388, 1192, 1657, 2022
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 9 cut(s) 167, 175, 389, 428, 704, 917, 1012, 1930, 1999
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 2 cut(s) 258, 1404
HphI GGTGA 3 cut(s) 213, 665, 1684
Hpy166II GTNNAC 4 cut(s) 388, 1192, 1657, 2022
Hpy8I GTNNAC 4 cut(s) 388, 1192, 1657, 2022
Hpy99I CGWCG 3 cut(s) 62, 109, 1487
HpyAV CCTTC 8 cut(s) 153, 301, 486, 757, 1295, 1609, 2026, 2036
HpyCH4III ACNGT 3 cut(s) 121, 571, 1529
HpyCH4IV ACGT 2 cut(s) 60, 1482
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 95, 446, 458, 783, 1771, 2126, 2204
HpyF3I CTNAG 5 cut(s) 647, 939, 1274, 1458, 1926
HpySE526I ACGT 2 cut(s) 60, 1482
Hsp92I GRCGYC 1 cut(s) 1482
Hsp92II CATG 7 cut(s) 540, 623, 713, 781, 852, 1144, 1692
HspAI GCGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 1809
KspAI GTTAAC 1 cut(s) 2022
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
LmnI GCTCC 2 cut(s) 860, 2243
Lsp1109I GCAGC 4 cut(s) 20, 461, 747, 1209
LweI GCATC 8 cut(s) 357, 802, 802, 892, 1783, 1927, 2095, 2133
MaeI CTAG 5 cut(s) 908, 998, 1638, 1934, 2096
MaeII ACGT 2 cut(s) 60, 1482
MaeIII GTNAC 5 cut(s) 340, 490, 841, 1870, 1908
MalI GATC 8 cut(s) 114, 1237, 1359, 1374, 1502, 1811, 2037, 2137
MbiI CCGCTC 1 cut(s) 2246
MboI GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
MfeI CAATTG 3 cut(s) 26, 687, 1903
MflI RGATCY 1 cut(s) 1500
MhlI GDGCHC 2 cut(s) 206, 646
MlsI TGGCCA 2 cut(s) 1032, 1693
MluI ACGCGT 1 cut(s) 868
MluNI TGGCCA 2 cut(s) 1032, 1693
MlyI GAGTC 4 cut(s) 176, 383, 437, 926
MmeI TCCRAC 2 cut(s) 946, 2221
Mox20I TGGCCA 2 cut(s) 1032, 1693
MroXI GAANNNNTTC 1 cut(s) 607
MscI TGGCCA 2 cut(s) 1032, 1693
MseI TTAA 9 cut(s) 321, 398, 515, 662, 1568, 1605, 1853, 2021, 2261
MslI CAYNNNNRTG 2 cut(s) 1075, 1749
Msp20I TGGCCA 2 cut(s) 1032, 1693
MspA1I CMGCKG 1 cut(s) 45
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 2 cut(s) 258, 1404
MspR9I CCNGG 7 cut(s) 798, 993, 1017, 1404, 1547, 1588, 2054
MunI CAATTG 3 cut(s) 26, 687, 1903
Mva1269I GAATGC 2 cut(s) 559, 1473
MvaI CCWGG 6 cut(s) 798, 993, 1017, 1547, 1588, 2054
MvnI CGCG 3 cut(s) 45, 109, 870
MwoI GCNNNNNNNGC 8 cut(s) 42, 95, 446, 458, 783, 1771, 2126, 2204
NciI CCSGG 1 cut(s) 1404
NdeI CATATG 3 cut(s) 1072, 1249, 1987
NdeII GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
NlaIII CATG 7 cut(s) 540, 623, 713, 781, 852, 1144, 1692
NlaIV GGNNCC 5 cut(s) 141, 204, 628, 1021, 1502
NmeAIII GCCGAG 2 cut(s) 162, 1741
NmuCI GTSAC 4 cut(s) 340, 490, 841, 1870
NspI RCATGY 4 cut(s) 540, 713, 781, 852
PaeI GCATGC 1 cut(s) 781
PciI ACATGT 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 2 cut(s) 559, 1473
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 5 cut(s) 175, 704, 1012, 1930, 1999
PflFI GACNNNGTC 1 cut(s) 371
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 5 cut(s) 35, 47, 451, 737, 1224
PleI GAGTC 4 cut(s) 175, 383, 436, 925
PpsI GAGTC 4 cut(s) 175, 383, 436, 925
PscI ACATGT 1 cut(s) 709
PsiI TTATAA 1 cut(s) 467
Psp6I CCWGG 6 cut(s) 796, 991, 1015, 1545, 1586, 2052
PspGI CCWGG 6 cut(s) 796, 991, 1015, 1545, 1586, 2052
PspN4I GGNNCC 5 cut(s) 141, 204, 628, 1021, 1502
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 7 cut(s) 132, 139, 202, 203, 325, 876, 1127
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 1500
PsyI GACNNNGTC 1 cut(s) 371
RsaI GTAC 5 cut(s) 161, 535, 547, 867, 1401
RsaNI GTAC 5 cut(s) 160, 534, 546, 866, 1400
RseI CAYNNNNRTG 2 cut(s) 1075, 1749
SacII CCGCGG 1 cut(s) 46
SalI GTCGAC 1 cut(s) 1190
SaqAI TTAA 9 cut(s) 321, 398, 515, 662, 1568, 1605, 1853, 2021, 2261
SatI GCNGC 5 cut(s) 34, 46, 450, 736, 1223
Sau3AI GATC 8 cut(s) 112, 1235, 1357, 1372, 1500, 1809, 2035, 2135
Sau96I GGNCC 7 cut(s) 132, 139, 202, 203, 325, 876, 1127
SchI GAGTC 4 cut(s) 176, 383, 437, 926
ScrFI CCNGG 7 cut(s) 798, 993, 1017, 1404, 1547, 1588, 2054
SduI GDGCHC 2 cut(s) 206, 646
SfaNI GCATC 8 cut(s) 357, 802, 802, 892, 1783, 1927, 2095, 2133
SfcI CTRYAG 1 cut(s) 1599
Sfr303I CCGCGG 1 cut(s) 46
SgrBI CCGCGG 1 cut(s) 46
SinI GGWCC 3 cut(s) 132, 139, 876
SmiMI CAYNNNNRTG 2 cut(s) 1075, 1749
SmlI CTYRAG 3 cut(s) 292, 320, 2147
SmoI CTYRAG 3 cut(s) 292, 320, 2147
SphI GCATGC 1 cut(s) 781
SsiI CCGC 6 cut(s) 43, 45, 89, 261, 1832, 2246
SspI AATATT 1 cut(s) 1611
SspMI CTAG 5 cut(s) 908, 998, 1638, 1934, 2096
StyD4I CCNGG 7 cut(s) 796, 991, 1015, 1402, 1545, 1586, 2052
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 3 cut(s) 121, 571, 1529
TaiI ACGT 2 cut(s) 63, 1485
TaqI TCGA 7 cut(s) 9, 115, 170, 1122, 1191, 1234, 1350
TatI WGTACW 1 cut(s) 533
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 5 cut(s) 175, 704, 1012, 1930, 1999
Tru1I TTAA 9 cut(s) 321, 398, 515, 662, 1568, 1605, 1853, 2021, 2261
Tru9I TTAA 9 cut(s) 321, 398, 515, 662, 1568, 1605, 1853, 2021, 2261
TscAI CASTG 7 cut(s) 213, 574, 591, 1030, 1387, 1875, 2145
TseFI GTSAC 4 cut(s) 340, 490, 841, 1870
TseI GCWGC 4 cut(s) 33, 449, 735, 1222
Tsp45I GTSAC 4 cut(s) 340, 490, 841, 1870
TspDTI ATGAA 4 cut(s) 165, 1157, 1309, 1947
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 7 cut(s) 213, 574, 591, 1030, 1387, 1875, 2145
Tth111I GACNNNGTC 1 cut(s) 371
Vha464I CTTAAG 1 cut(s) 320
VpaK11BI GGWCC 3 cut(s) 132, 139, 876
XagI CCTNNNNNAGG 1 cut(s) 1461
XapI RAATTY 6 cut(s) 603, 818, 1643, 1888, 1972, 2111
XbaI TCTAGA 2 cut(s) 1933, 2095
XceI RCATGY 4 cut(s) 540, 713, 781, 852
XmiI GTMKAC 1 cut(s) 1191
XmnI GAANNNNTTC 1 cut(s) 607
XspI CTAG 5 cut(s) 908, 998, 1638, 1934, 2096
ZraI GACGTC 1 cut(s) 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.