Rorug05G0598200

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009603
Physical Location & Seq
Forward (+)
79620860 .. 79650596
29737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0598200.1

Sequence Viewer

Length: 7035 bp
ATGACGTCTTGTCAATGTCTTGTGTGGTGTTGTCTTAACAGAGAAATGGAAGGAAACCAGGCACTAAATAATTTCAAAGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCTAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGGACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATTGGGCGCAGGCACATGTACACATAATGCTTTGTATTCAAATTCTGATGTGGGACTTTTCAAGAGTGATACAGATAAGTCGTTGGATGAACACCACCCATTTCCTGTCTTATTGATGCCGCGCAGTTTGCTTATATTCAAAGATAAAGCATACTCGGATTACTTGCATGCTATAAACGACAGTGAGGTACACAGTTATGATAAGGTTGTAAACGAAGCACAAGCTCGACCTTGGCAAGGCCTAGATCATCCATCTTCACAATTGGATGGAACTGTTGAAACATTGAATACTGTGGACCTCAAGAGTATTCATAGAACTACCACTAGAGTTTCTTTAACATGTCGATTAGTTTTGAAAGTTCATAAAAATTTGTTCAGAGTCGCAATTCGCAACTCGCAACGGGGAGGCGAAGAAAAGAAGGAAGCCCAGAAAGCAGGAAGGAGACACAGTAGGTTGCCTTATCTTGCACCTATTCAGGCAAGTCGTGTCGCCGCTGGTTCGACCCAAGCAACCAGATTTACAGCTGCTCGGCAGATAGGGGATATTGCCAAATCACATCCTCAGGACTTGACCTCTCTCTTAAAAAAGGTTTCACAATATCTTCGTAGCAGAAACTGGGACACAAGGGTTGCTGCTGCTCATGCAATTGGGGCAATTGCTGAGAATGTTAAGCATACCTCTGTAAATGAACTCTTTGCTTGCATTCAATCGAAAATGTGTGATGCTGGGATTTCTGGTGCTGTTGAAGACATGGTTGCTTTACCTATGTTTGATTCTAACATTGCAGGCACGTCGTTCAGAAGCTTTGATCTCAGCAAGGTTTTGGAGTTTGGTGCGCTTTTGGCATCTCGAGGACAGGAGTATGATATCGCAAATGATAATACAAAGAACCCAAGAGAGAGATTGGCTCGACAGAAACAAACACTTCGACGTCGTTTAGGTTTGGATATATGTGAGCAGTTCATGGATATGAATGATATGATAAGAGATGAAGACCTAATTTTGCACAACTCCCATGGGAACGGGATTAATTCGAGAGTTTATACCTCACGTAATATTCAGCAGTTGGTTGCAAATATGGTTCCTAGTGTTTTATCTAAAAGGCCAAGTCCAAGGGAATTGAATCTTCTAAAACGAAAAGCAAAAATCAATTCAAAAGACCAATCCAAGGGGTGGTCTGAGGATGGGGATATGGAGGTGGCCTGTGCTCAAAGTATGACCACACCAAAGGGCTCATGTCCTGATTCATTTGGCACTAATAAGGAATTTATGGAATTTGATCAAGAAGAAAACTTTGAACAAGATGGAGAGGGGCGGTGGCCTTTTCATAGTTTTGTTGAGCAACTCATCCTAGACATGTTTGATCCAGTTTGGGAAGTACGCCATGGTGGTGTGATGGCTTTGAGGGAAATTTTAACCCATCAGGGTGCTTCTGCCGGAGTATTTATGCCTGACTTGAGCCTAGATGATGCACTGTTTGTTGATTTAGAAAGTAAATGGACATCACAGACAATGAAGAGAAGCAGAGAGATTGATTTGAATTTGCAAGTTCCAATTGATGAATCTAAAACAATGGTGAAAAAACCGAAGTTTGAAGATGTTTCGTGTCCACTTTTGGAAACAATGGTCTCTGCCAGCAAGGATGGGAATGTTGATATCAGTATGCAGGTTGAAGATGGTGGGTGTAATTTGCCTTCTGAGCAAGTTAATGGTCAACTTTGTGTCAGCTCTCTAAAGGTGGAGCCTGAATTGTATCCATGTGAGCAACCGGTCAGTACAACTGAGTTGAAGTGTGAGGCATCCTCTCAGAAAATGGATCTGCTGAGAAGTCTAACTGAAAATAACGAGCTGATGAACTTGGTTAAACTGGTTAGGCATTCATGGCTTAAAAATTGTGAATTTCTTCAAGATTGTGCGATTCGTTTCCTGTGTGTATTGTCGCTAGACCGTTTTGGAGATTATGTCTCTGATCAGGTAGTTGCTCCCGTACGTGAAACCTGTGCACAAGCATTGGGTGTTGTGTTCAAGTACATGCATCCAACATTTGTTCATGAAACATTAAATATCTTGTTGAAGATGCAGTGTAGACCAGAATGGGAAATTCGTCATGGAAGCCTTTTGGGTATTAAGTATCTGGTGGCCGTCCGACAGGAAATGCTTCATAACTTACTTGATAGGGTTCTCCCTGCTTGTAAAGCTGGCCTAGAGGACCCTGATGATGATGTTCGAGCAGTAGCTGCAGATGCCTTAATACCTACTGCATCTGCTATTGTTGCATTAAAGGGTCAAACATTGCATTCTGTGGTGATGCTTCTTTGGGATATATTACTTGATTTGGATGATTTAAGTCCATCCACTAGCAGTGTAATGAATCTCCTGGCAGAAATTTACTCCCAAGAAGAGATGATCCCAAAGATTTTTGAGCCATTATCGTTGAAAGAGAACCTGGAGTTTGACTTGAATGAACTGGGTCGCATAGATGATGCTAGAGAAGGAATAATTTCACAGGATAATCCTTTCATGCTATCTACGCTGGCACCACGGTTGTGGCCTTTTATGAGGCATAGTATAACATCAGTCCGTTATTCAGCTATCCGCACTTTGGAGAGACTGCTTGAAGCAGGATACAGAAGGAACATTTCTGAGCCTTCGAGTACTTCGTTTTGGCCTTCCTTTATCCTGGGCGACACTCTCAGGATTGTTTTTCAGAATTTGCTACTTGAATCAAATGATGAAATTTTGAAACATTCGGAGAGGGTTTGGAGGCTCCTTGTTCAGTGTCCAGTAGGGGATTTGGAAATTGCTGCAAGGTCATATATGTCTTCTTGGATAGAACTTGCAACTACTTCATATGGTTCAGCATTAGATTCAGCAAAGATGTTTTGGCCTGTTACTCTTCCTCGAAAGAGTCATTTTAAAGCAGCAGCTAAAATGAGAGCTGTGAAGCTTGAAAATGAGTCTTGTGGAAACATTGGCTTGGAATCTGCAAAGGGGTCCATTTCACAGGAAAAAGCTGGAGATGCTTTAACCAATAATGTCCAGATAATTGTTGGTGCAGATGTTGAATTGTCAGTAACTCATACCAGAGTGGTTACAGCGGCAGCATTGGGAGTTTTTGCTTCTAGATTACAGGAAGGCTCTATACAGTATGTAATCGACCCGCTGACAAATGCCCTTACGTCTTTATCTGGTGTCCAGCGGCAGGTAGCATCTATGGTTCTTATTTCTTGGTTCAAAGAGATTAAAAGTAAGGGTGTATTTGATATTGCTGGAGTTATGCCAGGTTTTCCGAACCATATCAAAAGTTGGTTGTTGGATTTGTTAGCTTCTTCTGACCCTGCTTTTCCTACAAAAGATTCACTCCTTCCTTACACTGAGCTTTCGAAAACATATAGTAAGATGCGTGATCAGGCTAGTCAGTTGTTACATACTGTTGAGTCATCGGGTATGTTTGAGAGCTTCTTGTCCACTAATAAAATTCATTTGGAAAGCTTGAGCGTGGACGATGCGATTAATTTTGCATCAAAACTTCCTATGTTGTCTAATGATAATGTGGGGAACGATTCTTTGGAAAGGCATATCTTTGATGGTATAGAGTCAGCAAAGCAACAGCTTTTGACAACATCAGGATATTTAAAATGTGTTCAGAGCAATCTGCACGTTGCTGTTTCCTCCCTGGTTGCGGCTTCAGTTGTTTGGATGTCAGAGCTTCCTGCACGACTAAATCCTATTATCTTGCCTTTAATGGCTTCCATCAAAAGAGAACAGGAGGAAGTTCTTCAACAAAAGGCAGCTGAGGCACTTGCAGAGCTTATCTCTGATTGTATTTCTCGCAGGCCTAGCCCAAATGATAAGTTAATTAAGAATATTTGTAGTTTAACATGCATGGATCCTAGCGAGACACCACAAGCTGCAGTCCTCTGTTCCATAGATATTCTTGATGATCAAGACCTTCTTTCTTTGGGGACTAATAGTAGTAAGCAGAAAACAAAGGTTCATATGGTAGCTGGTAGTGAAGACCGTTCAAAGGTTGAAGGCTTTATTAGCAGACGAGGTTCTGAGCTTGCATTGAGGCATCTCTGTATGAAGTTTGGCACTTCATTATTTGATAAGCTTCCTAAACTATGGGATTGCCTCACGGAAGTTCTTAAGCCTAGTAGTATTGAGTCCCTCAGTCCTGCAGATGAAATTATAATTACACAAGCTATGGAATCTGTGAGGGATCCTCAGCTCTTGATAAACAATATCCAGGTGGTACGTTCTATAGCTCCTATGCTAAATGAGGATTTAAAACCAAAATTGCTCACTCTGCTTCCATACATCTTCAAGTGTGTGCGGCATTCTCATGTTGCTGTTCGACTAGCTTCGTCTAGATGCATCACTTCCATGGCCAAGTCCATGACGATACCTATGATGGGAGCTGTAATTGAAAATGCCATCCCAATGTTGGGGGATGTGACATCTGTTAATGCTCGACAAGGTGCAGGCATGCTTATTAGTTTGATTGTTCAGGGACTGGGTGTAGAGTTGGTTCCATATGCTCCTTTGTTGGTTGTTCCTCTTCTAAGATGTATGAGTGACTGTGATCAGTCTGTCAGACAGAGTGTGACACACAGTTTTGCTGCCCTTGTACCACTTCTTCCCCTGGCTCGTGGCCTCCCTCCACCTGTTGGGCTAAGTGAAGATTTCTCCAGAAGTGCAGAAGATGCAAAGTTTCTAGAGCAACTACTTGACAACTCTCATATTGATGATTACGAGCTTTGTACTGAACTCAAAGTGACCCTGAGGAGGTATCAACAGGAAGGTATAAATTGGTTGGCTTTTTTAAAACGTTTTAAGCTTCATGGAATCTTGTGCGATGATATGGGTCTTGGTAAGACACTTCAAGCATCTGCTATTGTGGCTTCCGACGTAGTTGAACATCGCACTTCAAATGATAGCAATATCCCACCATCTTTAATTATTTGCCCATCAACACTTGTTGCGCACTGGGCCTTCGAGATAGAGAAGTACATTGATGGTTCCGTTTTATCTACTCTCCAATATGTTGGTTCTGTTCAAGATCGCTCCAGTCTTCGAGAAAAGTTTGATAAGCATAATGTGATAATAACATCATATGATGTGGTTCGGAAAGATGTTGATTATCTTGGAAAGCTTCTCTGGAATTATTGTATCTTAGATGAAGGACATGTCATCAAGAATGCGAAGTCAAAAATAACACTTTCCGTGAAGCAATTGAAAGCTCATAATCGCTTGATACTGAGTGGAACACCAATTCAGAATAACATCATGGATTTGTGGTCCCTTTTTGATTTCCTAATGCCCGGGTTTCTTGGAACAGAGAGACAGTTCCAAGCCACTTATGGAAAACCACTGGTGGCAGCGAGGGATCCTAAATGCTCAGCCAAGGATGCTGAAGCAGGAGCACTTGCTATGGAAGCGTTGCACAAGCAGGTCATGCCTTTCCTCCTTCGTCGAACAAAAGATGAAGTCTTGTCTGATCTTCCTGAGAAAATCATTCAGGACAGATTCTGTGACTTGGGCCCTGTACAATTAAAATTGTATGAACAGTTTTCTGGTTCACATGTAAGACACGAGATATCAAGTATGGTGAAACTAAACGAGTCTGCAGATACAGGCGGGCGTACTGATTCACCCCGAGCATCGTCACACGTTTTTCAGGCACTTCAGTACTTGCTTAAACTTTGTAGCCATCCATTGCTTGTTCTTGGTGATAAGCTTCCAGACTCTACTGATTTCCTTTTGTCAGAGACATTGCCTGGCGTCTCTGACATCATTTCGGAATTGCATAAGCCATACCACTCTCCCAAGCTGGTTGCACTTCAAGAGATTCTGGAAGAGTGTGGAATAGGTGTCGACGCTTCTGGTTCTGAAGGTGCCGTGGGTGTTGGGCAGCACAGAGTCTTGATTTTTGCTCAACATAAAGCATTTCTGGACTTAATTGAAAGAGACTTGTTTCATGCTCATATGAAGAGTGTCACCTACTTGCGGCTGGATGGGTCAGTTGAACCTGAGAAACGTTTTGATATTGTTAAAGCTTTCAACTCAGATCCTACAATTGATGTCCTGCTTCTCACAACTCATGTTGGTGGGCTGGGCTTGAACCTGACATCTGCTGACACACTCGTTTTCATGGAGCACGACTGGAACCCAATGCGGGATCATCAGGCAATGGACAGGGCACACAGATTAGGTCAGAAAAAGGTTGTCAATGTCCATCGTCTGATTATGCGTGGCACTCTTGAAGAGAAAGTTATGAGCCTGCAAAAGTTTAAGCTCTCTGTAGCTAATGCAGTCATTAATGCAGAGAATGCTAGTATGAAAACAATGAATACAGACCAATTGCTTGATCTCTTTGCAACAGCAGAAACATCTAAGAAGGGAACTTCTGTATCCAAGTATCCGGATGGAAAGTTTGATGGAGATGTGAAATTAACGGGCGCTGGAAAGGGGTTGAAAGCAATTCTTGGCGGGCTGGAAGAGCTGTGGGATCAATCACAATACACTGAAGAATACAATCTTAGCCAGTTCTTAGCAAAGCTTGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

2344

Amino Acids

260.9

Weight (kDa)

5.93

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 67 - 269 6.6e-09 2OG-Fe(II) oxygenase superfamily
DUF3535 PF12054 1074 - 1532 1.1e-100 Domain of unknown function (DUF3535)
SNF2-rel_dom PF00176 1752 - 2060 3.8e-69 SNF2-related domain
Helicase_C PF00271 2126 - 2226 5.3e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4655
AatII GACGTC 2 cut(s) 8, 1396
Acc16I TGCGCA 1 cut(s) 5448
Acc36I ACCTGC 3 cut(s) 2119, 3655, 5905
AccB1I GGYRCC 2 cut(s) 2998, 6359
AccB7I CCANNNNNTGG 2 cut(s) 1635, 5132
AccI GTMKAC 2 cut(s) 2548, 6339
AccII CGCG 1 cut(s) 583
AccIII TCCGGA 1 cut(s) 6886
AclI AACGTT 2 cut(s) 5293, 6502
AcoI YGGCCR 2 cut(s) 2601, 4851
AcuI CTGAAG 5 cut(s) 4134, 5898, 6134, 6375, 7011
AcyI GRCGYC 3 cut(s) 5, 1393, 6246
AflII CTTAAG 1 cut(s) 4610
AflIII ACRYGT 6 cut(s) 476, 800, 1818, 5650, 6046, 6133
AgeI ACCGGT 1 cut(s) 2230
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 1254
AjuI GAANNNNNNNTTGG 2 cut(s) 4013, 4045
AleI CACNNNNGTG 1 cut(s) 3007
Alw21I GWGCWC 4 cut(s) 1672, 2467, 5890, 6624
Alw44I GTGCAC 1 cut(s) 2463
AlwNI CAGNNNCTG 4 cut(s) 1077, 2699, 4978, 5994
Ama87I CYCGRG 3 cut(s) 1311, 5786, 6120
Aor13HI TCCGGA 1 cut(s) 6886
ApaI GGGCCC 1 cut(s) 6008
ApaLI GTGCAC 1 cut(s) 2463
AseI ATTAAT 3 cut(s) 1491, 3975, 6783
AsiGI ACCGGT 1 cut(s) 2230
Asp700I GAANNNNTTC 5 cut(s) 345, 2364, 2619, 2962, 4239
AspLEI GCGC 5 cut(s) 470, 585, 1300, 5449, 6926
AspS9I GGNCC 8 cut(s) 380, 757, 2671, 3456, 5454, 5763, 6004, 6005
AsuC2I CCSGG 2 cut(s) 5787, 5788
AsuHPI GGTGA 7 cut(s) 414, 2050, 2779, 6085, 6108, 6206, 6454
AsuII TTCGAA 1 cut(s) 3845
AvaI CYCGRG 3 cut(s) 1311, 5786, 6120
AvaII GGWCC 4 cut(s) 757, 2671, 3456, 5763
AxyI CCTNAGG 2 cut(s) 1023, 5246
BaeGI GKGCMC 3 cut(s) 2467, 6008, 6667
BaeI ACNNNNGTAYC 2 cut(s) 6858, 6891
BalI TGGCCA 1 cut(s) 4853
BamHI GGATCC 3 cut(s) 4351, 4684, 5851
BanI GGYRCC 2 cut(s) 2998, 6359
BanII GRGCYC 2 cut(s) 1697, 6008
BarI GAAGNNNNNNTAC 8 cut(s) 123, 155, 2140, 2172, 3588, 3620, 5085, 5117
BauI CACGAG 2 cut(s) 5112, 6056
BbsI GAAGAC 5 cut(s) 1215, 1461, 3276, 4485, 5528
Bbv12I GWGCWC 4 cut(s) 1672, 2467, 5890, 6624
BbvCI CCTCAGC 2 cut(s) 4257, 4689
BceAI ACGGC 2 cut(s) 2588, 6347
BcgI CGANNNNNNTGC 2 cut(s) 1236, 1270
BciVI GTATCC 4 cut(s) 2226, 3080, 6886, 6894
BclI TGATCA 5 cut(s) 1741, 2431, 3868, 4405, 5047
BcnI CCSGG 2 cut(s) 5787, 5788
BfmI CTRYAG 6 cut(s) 2700, 4374, 4641, 4725, 6090, 6765
BfoI RGCGCY 1 cut(s) 6927
BfrI CTTAAG 1 cut(s) 4610
BfuAI ACCTGC 3 cut(s) 2119, 3655, 5905
BfuI GTATCC 4 cut(s) 2226, 3080, 6886, 6894
BlpI GCTNAGC 1 cut(s) 5863
BmcAI AGTACT 2 cut(s) 3118, 6155
Bme18I GGWCC 4 cut(s) 757, 2671, 3456, 5763
BmeRI GACNNNNNGTC 1 cut(s) 9
BmeT110I CYCGRG 3 cut(s) 1311, 5786, 6120
BmgBI CACGTC 1 cut(s) 1254
BmgT120I GGNCC 8 cut(s) 380, 757, 2671, 3456, 5454, 5763, 6004, 6005
BmrI ACTGGG 5 cut(s) 220, 1087, 2939, 4988, 5461
BmuI ACTGGG 5 cut(s) 220, 1087, 2939, 4988, 5461
BpiI GAAGAC 5 cut(s) 1215, 1461, 3276, 4485, 5528
BplI GAGNNNNNCTC 8 cut(s) 1354, 1386, 2249, 2281, 4262, 4294, 4672, 4704
BpmI CTGGAG 5 cut(s) 2930, 3498, 3753, 5137, 5515
Bpu10I CCTNAGC 2 cut(s) 4257, 4689
Bpu1102I GCTNAGC 1 cut(s) 5863
Bpu14I TTCGAA 1 cut(s) 3845
BpuEI CTTGAG 3 cut(s) 746, 1939, 3976
BpuMI CCSGG 2 cut(s) 5787, 5788
BsaAI YACGTR 2 cut(s) 1514, 2453
BsaHI GRCGYC 3 cut(s) 5, 1393, 6246
BsaI GGTCTC 1 cut(s) 2095
BsaWI WCCGGW 2 cut(s) 2230, 6886
BsaXI ACNNNNNCTCC 4 cut(s) 895, 925, 2409, 2439
Bse118I RCCGGY 1 cut(s) 2230
Bse21I CCTNAGG 2 cut(s) 1023, 5246
Bse3DI GCAATG 5 cut(s) 1242, 2753, 6179, 6236, 6660
BseAI TCCGGA 1 cut(s) 6886
BseMI GCAATG 5 cut(s) 1242, 2753, 6179, 6236, 6660
BseRI GAGGAG 1 cut(s) 5263
BseSI GKGCMC 3 cut(s) 2467, 6008, 6667
BseYI CCCAGC 2 cut(s) 1187, 6577
BsgI GTGCAG 5 cut(s) 3537, 4103, 4161, 4965, 5181
Bsh1236I CGCG 1 cut(s) 583
BshNI GGYRCC 2 cut(s) 2998, 6359
BshTI ACCGGT 1 cut(s) 2230
BsiHKAI GWGCWC 4 cut(s) 1672, 2467, 5890, 6624
BsiHKCI CYCGRG 3 cut(s) 1311, 5786, 6120
BsiSI CCGG 4 cut(s) 1899, 2231, 5787, 6887
BsiWI CGTACG 1 cut(s) 2449
BslFI GGGAC 7 cut(s) 381, 528, 1094, 4441, 4615, 4989, 5749
BsmBI CGTCTC 1 cut(s) 6253
BsmFI GGGAC 7 cut(s) 381, 528, 1094, 4441, 4615, 4989, 5749
BsmI GAATGC 6 cut(s) 1164, 2338, 2758, 4801, 5668, 6799
Bso31I GGTCTC 1 cut(s) 2095
BsoBI CYCGRG 3 cut(s) 1311, 5786, 6120
Bsp119I TTCGAA 1 cut(s) 3845
Bsp120I GGGCCC 1 cut(s) 6004
Bsp1286I GDGCHC 7 cut(s) 1672, 1697, 2467, 5890, 6008, 6624, 6667
Bsp13I TCCGGA 1 cut(s) 6886
Bsp1407I TGTACA 2 cut(s) 479, 6010
Bsp1720I GCTNAGC 1 cut(s) 5863
Bsp19I CCATGG 3 cut(s) 1477, 1846, 4848
BspEI TCCGGA 1 cut(s) 6886
BspFNI CGCG 1 cut(s) 583
BspHI TCATGA 2 cut(s) 226, 2512
BspMAI CTGCAG 4 cut(s) 2704, 4378, 4645, 6094
BspMI ACCTGC 3 cut(s) 2119, 3655, 5905
BspQI GCTCTTC 1 cut(s) 6957
BspT104I TTCGAA 1 cut(s) 3845
BspT107I GGYRCC 2 cut(s) 2998, 6359
BspTI CTTAAG 1 cut(s) 4610
BspTNI GGTCTC 1 cut(s) 2095
BsrDI GCAATG 5 cut(s) 1242, 2753, 6179, 6236, 6660
BsrFI RCCGGY 1 cut(s) 2230
BsrGI TGTACA 2 cut(s) 479, 6010
BssAI RCCGGY 1 cut(s) 2230
BssNI GRCGYC 3 cut(s) 5, 1393, 6246
BssSI CACGAG 2 cut(s) 5112, 6056
BssT1I CCWWGG 8 cut(s) 355, 692, 1477, 1574, 1629, 1846, 4848, 5868
Bst2BI CACGAG 2 cut(s) 5112, 6056
Bst6I CTCTTC 8 cut(s) 1973, 2856, 3363, 5028, 6315, 6449, 6723, 6957
BstACI GRCGYC 3 cut(s) 5, 1393, 6246
BstAFI CTTAAG 1 cut(s) 4610
BstAPI GCANNNNNTGC 4 cut(s) 2699, 5168, 5920, 6794
BstAUI TGTACA 2 cut(s) 479, 6010
BstBAI YACGTR 2 cut(s) 1514, 2453
BstBI TTCGAA 1 cut(s) 3845
BstDSI CCRYGG 5 cut(s) 1477, 1846, 3002, 4848, 6363
BstEII GGTNACC 1 cut(s) 420
BstENI CCTNNNNNAGG 2 cut(s) 354, 696
BstFNI CGCG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 6927
BstHHI GCGC 5 cut(s) 470, 585, 1300, 5449, 6926
BstPI GGTNACC 1 cut(s) 420
BstSFI CTRYAG 6 cut(s) 2700, 4374, 4641, 4725, 6090, 6765
BstSLI GKGCMC 3 cut(s) 2467, 6008, 6667
BstUI CGCG 1 cut(s) 583
BstV2I GAAGAC 5 cut(s) 1215, 1461, 3276, 4485, 5528
BstX2I RGATCY 6 cut(s) 83, 2278, 4351, 4684, 5851, 6532
BstXI CCANNNNNNTGG 2 cut(s) 3009, 5510
BstYI RGATCY 6 cut(s) 83, 2278, 4351, 4684, 5851, 6532
Bsu36I CCTNAGG 2 cut(s) 1023, 5246
BsuI GTATCC 4 cut(s) 2226, 3080, 6886, 6894
BtgI CCRYGG 5 cut(s) 1477, 1846, 3002, 4848, 6363
BtgZI GCGATG 3 cut(s) 297, 5334, 5369
BtrI CACGTC 1 cut(s) 1254
BtsI GCAGTG 2 cut(s) 2549, 2830
BtsIMutI CAGTG 9 cut(s) 649, 1934, 2549, 2830, 3245, 3834, 5449, 5834, 6987
BveI ACCTGC 3 cut(s) 2119, 3655, 5905
CaiI CAGNNNCTG 4 cut(s) 1077, 2699, 4978, 5994
CciI TCATGA 2 cut(s) 226, 2512
CfoI GCGC 5 cut(s) 470, 585, 1300, 5449, 6926
Cfr10I RCCGGY 1 cut(s) 2230
Cfr13I GGNCC 8 cut(s) 380, 757, 2671, 3456, 5454, 5763, 6004, 6005
Cfr9I CCCGGG 1 cut(s) 5786
CseI GACGC 2 cut(s) 6235, 6350
CspAI ACCGGT 1 cut(s) 2230
DraI TTTAAA 5 cut(s) 189, 3379, 4098, 4752, 5289
DriI GACNNNNNGTC 1 cut(s) 9
EaeI YGGCCR 2 cut(s) 2601, 4851
Eam1104I CTCTTC 8 cut(s) 1973, 2856, 3363, 5028, 6315, 6449, 6723, 6957
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 8 cut(s) 1973, 2856, 3363, 5028, 6315, 6449, 6723, 6957
Eco130I CCWWGG 8 cut(s) 355, 692, 1477, 1574, 1629, 1846, 4848, 5868
Eco147I AGGCCT 2 cut(s) 702, 4300
Eco24I GRGCYC 2 cut(s) 1697, 6008
Eco31I GGTCTC 1 cut(s) 2095
Eco32I GATATC 4 cut(s) 243, 1330, 2119, 6063
Eco47I GGWCC 4 cut(s) 757, 2671, 3456, 5763
Eco57I CTGAAG 5 cut(s) 4134, 5898, 6134, 6375, 7011
Eco81I CCTNAGG 2 cut(s) 1023, 5246
Eco88I CYCGRG 3 cut(s) 1311, 5786, 6120
Eco91I GGTNACC 1 cut(s) 420
EcoNI CCTNNNNNAGG 2 cut(s) 354, 696
EcoO109I RGGNCCY 2 cut(s) 2671, 6005
EcoO65I GGTNACC 1 cut(s) 420
EcoRV GATATC 4 cut(s) 243, 1330, 2119, 6063
EcoT14I CCWWGG 8 cut(s) 355, 692, 1477, 1574, 1629, 1846, 4848, 5868
EcoT22I ATGCAT 3 cut(s) 2499, 4349, 4841
EcoT38I GRGCYC 2 cut(s) 1697, 6008
ErhI CCWWGG 8 cut(s) 355, 692, 1477, 1574, 1629, 1846, 4848, 5868
Esp3I CGTCTC 1 cut(s) 6253
FalI AAGNNNNNCTT 4 cut(s) 4401, 4433, 6933, 6965
FaqI GGGAC 7 cut(s) 381, 528, 1094, 4441, 4615, 4989, 5749
FauI CCCGC 4 cut(s) 3630, 6095, 6633, 6947
FauNDI CATATG 5 cut(s) 3313, 4461, 4999, 5578, 6450
FbaI TGATCA 5 cut(s) 1741, 2431, 3868, 4405, 5047
FblI GTMKAC 2 cut(s) 2548, 6339
FriOI GRGCYC 2 cut(s) 1697, 6008
FspI TGCGCA 1 cut(s) 5448
GlaI GCGC 5 cut(s) 469, 584, 1299, 5448, 6925
GsaI CCCAGC 2 cut(s) 1191, 6581
GsuI CTGGAG 5 cut(s) 2930, 3498, 3753, 5137, 5515
HaeII RGCGCY 1 cut(s) 6927
HapII CCGG 4 cut(s) 1899, 2231, 5787, 6887
HgaI GACGC 2 cut(s) 6235, 6350
HhaI GCGC 5 cut(s) 470, 585, 1300, 5449, 6926
Hin1I GRCGYC 3 cut(s) 5, 1393, 6246
Hin6I GCGC 5 cut(s) 468, 583, 1298, 5447, 6924
HinP1I GCGC 5 cut(s) 468, 583, 1298, 5447, 6924
HincII GTYRAC 3 cut(s) 267, 2177, 6340
HindII GTYRAC 3 cut(s) 267, 2177, 6340
HindIII AAGCTT 9 cut(s) 1264, 3407, 3952, 4574, 5300, 5615, 6200, 6519, 7022
HpaI GTTAAC 1 cut(s) 267
HpaII CCGG 4 cut(s) 1899, 2231, 5787, 6887
HphI GGTGA 7 cut(s) 414, 2050, 2779, 6085, 6108, 6206, 6454
Hpy99I CGWCG 6 cut(s) 1258, 1395, 1398, 5375, 5940, 6344
Hsp92I GRCGYC 3 cut(s) 5, 1393, 6246
HspAI GCGC 5 cut(s) 468, 583, 1298, 5447, 6924
Kpn2I TCCGGA 1 cut(s) 6886
Ksp22I TGATCA 5 cut(s) 1741, 2431, 3868, 4405, 5047
KspAI GTTAAC 1 cut(s) 267
LguI GCTCTTC 1 cut(s) 6957
LmnI GCTCC 9 cut(s) 2203, 2449, 3234, 4735, 4880, 5008, 5534, 5885, 6619
MfeI CAATTG 7 cut(s) 722, 1107, 1116, 2016, 5696, 6540, 6824
MflI RGATCY 6 cut(s) 83, 2278, 4351, 4684, 5851, 6532
MhlI GDGCHC 7 cut(s) 1672, 1697, 2467, 5890, 6008, 6624, 6667
MlsI TGGCCA 1 cut(s) 4853
MluNI TGGCCA 1 cut(s) 4853
MlyI GAGTC 9 cut(s) 849, 3379, 3428, 3908, 4067, 4637, 6095, 6203, 6393
MmeI TCCRAC 6 cut(s) 61, 525, 2525, 2632, 3756, 5394
Mox20I TGGCCA 1 cut(s) 4853
Mph1103I ATGCAT 3 cut(s) 2499, 4349, 4841
MroI TCCGGA 1 cut(s) 6886
MroXI GAANNNNTTC 5 cut(s) 345, 2364, 2619, 2962, 4239
MscI TGGCCA 1 cut(s) 4853
Msp20I TGGCCA 1 cut(s) 4853
MspA1I CMGCKG 6 cut(s) 956, 986, 3560, 3625, 3661, 4256
MspCI CTTAAG 1 cut(s) 4610
MspI CCGG 4 cut(s) 1899, 2231, 5787, 6887
MunI CAATTG 7 cut(s) 722, 1107, 1116, 2016, 5696, 6540, 6824
Mva1269I GAATGC 6 cut(s) 1164, 2338, 2758, 4801, 5668, 6799
MvnI CGCG 1 cut(s) 583
NciI CCSGG 2 cut(s) 5787, 5788
NcoI CCATGG 3 cut(s) 1477, 1846, 4848
NdeI CATATG 5 cut(s) 3313, 4461, 4999, 5578, 6450
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 8 cut(s) 420, 4918, 5039, 5068, 5239, 5996, 6129, 6460
NsbI TGCGCA 1 cut(s) 5448
NsiI ATGCAT 3 cut(s) 2499, 4349, 4841
NspV TTCGAA 1 cut(s) 3845
OliI CACNNNNGTG 1 cut(s) 3007
PacI TTAATTAA 1 cut(s) 4323
PaeI GCATGC 2 cut(s) 632, 4954
PaeR7I CTCGAG 1 cut(s) 1311
PagI TCATGA 2 cut(s) 226, 2512
PceI AGGCCT 2 cut(s) 702, 4300
PciI ACATGT 5 cut(s) 476, 800, 1818, 5650, 6046
PciSI GCTCTTC 1 cut(s) 6957
PcsI WCGNNNNNNNCGW 1 cut(s) 3119
PctI GAATGC 6 cut(s) 1164, 2338, 2758, 4801, 5668, 6799
PdmI GAANNNNTTC 5 cut(s) 345, 2364, 2619, 2962, 4239
Pfl23II CGTACG 1 cut(s) 2449
PflMI CCANNNNNTGG 2 cut(s) 1635, 5132
PinAI ACCGGT 1 cut(s) 2230
PleI GAGTC 9 cut(s) 848, 3378, 3427, 3907, 4066, 4636, 6094, 6203, 6392
PpsI GAGTC 9 cut(s) 848, 3378, 3427, 3907, 4066, 4636, 6094, 6203, 6392
Ppu21I YACGTR 2 cut(s) 1514, 2453
PpuMI RGGWCCY 1 cut(s) 2671
PscI ACATGT 5 cut(s) 476, 800, 1818, 5650, 6046
PshBI ATTAAT 3 cut(s) 1491, 3975, 6783
PsiI TTATAA 1 cut(s) 4655
Psp1406I AACGTT 2 cut(s) 5293, 6502
Psp5II RGGWCCY 1 cut(s) 2671
PspEI GGTNACC 1 cut(s) 420
PspFI CCCAGC 2 cut(s) 1187, 6577
PspLI CGTACG 1 cut(s) 2449
PspOMI GGGCCC 1 cut(s) 6004
PspPI GGNCC 8 cut(s) 380, 757, 2671, 3456, 5454, 5763, 6004, 6005
PspPPI RGGWCCY 1 cut(s) 2671
PsrI GAACNNNNNNTAC 2 cut(s) 4977, 5009
PstI CTGCAG 4 cut(s) 2704, 4378, 4645, 6094
PstNI CAGNNNCTG 4 cut(s) 1077, 2699, 4978, 5994
PsuI RGATCY 6 cut(s) 83, 2278, 4351, 4684, 5851, 6532
PvuII CAGCTG 2 cut(s) 986, 4256
SalI GTCGAC 1 cut(s) 6338
SapI GCTCTTC 1 cut(s) 6957
Sau96I GGNCC 8 cut(s) 380, 757, 2671, 3456, 5454, 5763, 6004, 6005
ScaI AGTACT 2 cut(s) 3118, 6155
SchI GAGTC 9 cut(s) 849, 3379, 3428, 3908, 4067, 4637, 6095, 6203, 6393
SduI GDGCHC 7 cut(s) 1672, 1697, 2467, 5890, 6008, 6624, 6667
SfcI CTRYAG 6 cut(s) 2700, 4374, 4641, 4725, 6090, 6765
Sfr274I CTCGAG 1 cut(s) 1311
SfuI TTCGAA 1 cut(s) 3845
SinI GGWCC 4 cut(s) 757, 2671, 3456, 5763
SlaI CTCGAG 1 cut(s) 1311
SmaI CCCGGG 1 cut(s) 5788
SmlI CTYRAG 5 cut(s) 761, 1311, 1918, 3955, 4610
SmoI CTYRAG 5 cut(s) 761, 1311, 1918, 3955, 4610
SphI GCATGC 2 cut(s) 632, 4954
SseBI AGGCCT 2 cut(s) 702, 4300
SspI AATATT 3 cut(s) 154, 1519, 4330
StuI AGGCCT 2 cut(s) 702, 4300
StyI CCWWGG 8 cut(s) 355, 692, 1477, 1574, 1629, 1846, 4848, 5868
TatI WGTACW 8 cut(s) 479, 2237, 2490, 3116, 5225, 5472, 6010, 6153
TauI GCSGC 7 cut(s) 583, 956, 3563, 3664, 4148, 4801, 6475
TscAI CASTG 9 cut(s) 649, 1941, 2549, 2830, 3245, 3841, 5456, 5841, 6994
TseFI GTSAC 8 cut(s) 420, 4918, 5039, 5068, 5239, 5996, 6129, 6460
Tsp45I GTSAC 8 cut(s) 420, 4918, 5039, 5068, 5239, 5996, 6129, 6460
TspGWI ACGGA 4 cut(s) 3032, 4616, 5476, 5677
TspMI CCCGGG 1 cut(s) 5786
TspRI CASTG 9 cut(s) 649, 1941, 2549, 2830, 3245, 3841, 5456, 5841, 6994
Van91I CCANNNNNTGG 2 cut(s) 1635, 5132
Vha464I CTTAAG 1 cut(s) 4610
VneI GTGCAC 1 cut(s) 2463
VpaK11BI GGWCC 4 cut(s) 757, 2671, 3456, 5763
VspI ATTAAT 3 cut(s) 1491, 3975, 6783
XagI CCTNNNNNAGG 2 cut(s) 354, 696
XbaI TCTAGA 3 cut(s) 3584, 4832, 5179
XcmI CCANNNNNNNNNTGG 3 cut(s) 3509, 4906, 5822
XhoI CTCGAG 1 cut(s) 1311
XmaI CCCGGG 1 cut(s) 5786
XmiI GTMKAC 2 cut(s) 2548, 6339
XmnI GAANNNNTTC 5 cut(s) 345, 2364, 2619, 2962, 4239
ZraI GACGTC 2 cut(s) 6, 1394
ZrmI AGTACT 2 cut(s) 3118, 6155
Zsp2I ATGCAT 3 cut(s) 2499, 4349, 4841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.