AT3G21310

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008248
Physical Location & Seq
Forward (+)
7497240 .. 7500323
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21310.1

Sequence Viewer

Length: 1152 bp
ATGGGGATGGAGGAAGGGATTAAAGATAATGCTGCATCAGCTCCCAATTCAAGACCTACAAACCAACTTAAAGCTCTTTTACCGATGCGGGTGCTTCAAGTTTTCTTGTTGTTCTTTGTTTTGGTTCTTGGAATCTCAGTCATTAGTATGCACATGATCAAGTACTTAAAGATTCAAACTTTAGCTCCATCGACATTGATCTCTACTTATGATGAGAGAATTACGTTAGAGAGCCTTATCAAGCCTCCATTGAATGGTTGGCATTCCATGAATGACAGTGAGCTTCTTTGGCGTGCTTCGATGGAGCCTCGGATACTTGACTATCCATTTAAAAGAGTTCCTAAAATGGCATTCATGTTTCTCACCAAAGGACCTTTGCCATTTGCTCCACTTTGGGAAAGGTTTTTCAAAGGGCATGAGGGCTTTTACTCAATCTATGTTCATACTTTGCCAAATTATAGATCAGATTTCCCAAGCTCATCTGTGTTTTACAGAAGACAGATCCCAAGTCAGCATGTAGCTTGGGGAGAGATGAGTATGTGTGATGCTGAAAGGAGGCTTTTGGCTAACGCGTTGCTCGATATCTCTAACGAATGGTTTGTTTTACTATCTGAAGCGTGTATTCCTCTTCGCGGTTTCAACTTTGTCTACCGTTATGTCTCTAGATCAAGATATAGTTTCATGGGTTCTGTTGACGAGGACGGGCCTTACGGGAGAGGCAGATATAGCTATGCAATGGGACCAGAAGTAAGTCTAAACGAGTGGAGAAAAGGGTCTCAGTGGTTTGAAATAAACAGAGCACTTGCTGTTGATATTGTTGAAGACATGGTTTACTATAACAAATTCAAAGAGTTTTGTAGACCTCCTTGTTATGTAGATGAACATTACTTCCCAACAATGCTCTCTATTGGATATCCGGATTTTCTCGCTAATCGGACATTGACTTGGACAGATTGGTCAAGGGGTGGTGCTCATCCAGCTACTTTTGGCAAGGCTGATATAACAGAGAAGTTCATCAAGAAGCTGTCAAGAGGTAAAGCTTGCTTCTACAATGATCAGCCATCTCAAGTTTGTTATCTTTTTGCAAGAAAGTTTGCTCCAAGCGCATTGAAGCCTTTACTTAAACTTGCACCAAAGGTTCTTGGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

383

Amino Acids

44.28

Weight (kDa)

9.18

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 116 - 342 4e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 955
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 2 cut(s) 648, 859
AccII CGCG 2 cut(s) 572, 633
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 2 cut(s) 88, 633
AclWI GGATC 1 cut(s) 496
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 254, 632
AflIII ACRYGT 1 cut(s) 570
AloI GAACNNNNNNTCC 2 cut(s) 873, 905
Alw21I GWGCWC 2 cut(s) 802, 973
Alw26I GTCTC 2 cut(s) 664, 780
AlwI GGATC 1 cut(s) 496
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 842
AspLEI GCGC 1 cut(s) 1106
AspS9I GGNCC 3 cut(s) 371, 704, 740
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 371, 740
BbsI GAAGAC 2 cut(s) 502, 828
Bbv12I GWGCWC 2 cut(s) 802, 973
BbvI GCAGC 1 cut(s) 19
BccI CCATC 3 cut(s) 196, 295, 1069
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciVI GTATCC 1 cut(s) 306
BclI TGATCA 2 cut(s) 156, 1054
BcoDI GTCTC 2 cut(s) 664, 780
BfaI CTAG 1 cut(s) 663
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
BmcAI AGTACT 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 371, 740
BmgT120I GGNCC 3 cut(s) 371, 704, 740
BmiI GGNNCC 2 cut(s) 306, 741
BmsI GCATC 3 cut(s) 44, 75, 535
BpiI GAAGAC 2 cut(s) 502, 828
BpuEI CTTGAG 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 308
BsaWI WCCGGW 1 cut(s) 916
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 632
Bse3DI GCAATG 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 916
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 2 cut(s) 12, 973
BseLI CCNNNNNNNGG 2 cut(s) 254, 632
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 150, 791
BseXI GCAGC 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 572, 633
BshFI GGCC 1 cut(s) 706
BsiHKAI GWGCWC 2 cut(s) 802, 973
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 2 cut(s) 254, 632
BsmAI GTCTC 2 cut(s) 664, 780
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 2 cut(s) 262, 350
BsnI GGCC 1 cut(s) 706
Bso31I GGTCTC 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 802, 973
Bsp13I TCCGGA 1 cut(s) 916
Bsp143I GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
BspACI CCGC 2 cut(s) 88, 633
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 2 cut(s) 149, 790
BspEI TCCGGA 1 cut(s) 916
BspFNI CGCG 2 cut(s) 572, 633
BspLI GGNNCC 2 cut(s) 306, 741
BspPI GGATC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 780
BsrDI GCAATG 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
Bst4CI ACNGT 2 cut(s) 278, 653
Bst6I CTCTTC 1 cut(s) 633
BstC8I GCNNGC 2 cut(s) 294, 1042
BstDEI CTNAG 2 cut(s) 136, 777
BstF5I GGATG 2 cut(s) 12, 973
BstFNI CGCG 2 cut(s) 572, 633
BstHHI GCGC 1 cut(s) 1106
BstKTI GATC 6 cut(s) 159, 201, 464, 504, 668, 1057
BstMAI GTCTC 2 cut(s) 664, 780
BstMBI GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
BstMWI GCNNNNNNNGC 5 cut(s) 38, 289, 726, 977, 1103
BstNSI RCATGY 1 cut(s) 518
BstUI CGCG 2 cut(s) 572, 633
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 2 cut(s) 502, 828
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 1 cut(s) 706
BtsCI GGATG 2 cut(s) 12, 973
BtsIMutI CAGTG 2 cut(s) 283, 785
Cac8I GCNNGC 2 cut(s) 294, 1042
CfoI GCGC 1 cut(s) 1106
Cfr13I GGNCC 3 cut(s) 371, 704, 740
Csp6I GTAC 1 cut(s) 163
CviAII CATG 7 cut(s) 154, 268, 355, 416, 515, 682, 826
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 136, 777
DpnI GATC 6 cut(s) 158, 200, 463, 503, 667, 1056
DpnII GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
DraI TTTAAA 1 cut(s) 331
DrdI GACNNNNNNGTC 1 cut(s) 955
DseDI GACNNNNNNGTC 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Eco31I GGTCTC 1 cut(s) 780
Eco32I GATATC 2 cut(s) 583, 914
Eco47I GGWCC 2 cut(s) 371, 740
Eco57I CTGAAG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 371
EcoRV GATATC 2 cut(s) 583, 914
FaeI CATG 7 cut(s) 157, 271, 358, 419, 518, 685, 829
FaqI GGGAC 1 cut(s) 753
FatI CATG 7 cut(s) 153, 267, 354, 415, 514, 681, 825
FauI CCCGC 1 cut(s) 81
FbaI TGATCA 2 cut(s) 156, 1054
FblI GTMKAC 2 cut(s) 648, 859
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 2 cut(s) 19, 960
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 663
GlaI GCGC 1 cut(s) 1105
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 1 cut(s) 706
HapII CCGG 1 cut(s) 917
HhaI GCGC 1 cut(s) 1106
Hin1II CATG 7 cut(s) 157, 271, 358, 419, 518, 685, 829
Hin6I GCGC 1 cut(s) 1104
HinP1I GCGC 1 cut(s) 1104
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 1 cut(s) 1038
HinfI GANTC 2 cut(s) 132, 172
HpaII CCGG 1 cut(s) 917
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 4 cut(s) 649, 694, 832, 860
Hpy188I TCNGA 4 cut(s) 312, 466, 613, 936
Hpy188III TCNNGA 6 cut(s) 51, 663, 669, 917, 1018, 1029
Hpy8I GTNNAC 4 cut(s) 649, 694, 832, 860
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 278, 653
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 5 cut(s) 35, 151, 734, 1085, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 289, 726, 977, 1103
HpyF3I CTNAG 2 cut(s) 136, 777
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 7 cut(s) 157, 271, 358, 419, 518, 685, 829
HspAI GCGC 1 cut(s) 1104
Kpn2I TCCGGA 1 cut(s) 916
Ksp22I TGATCA 2 cut(s) 156, 1054
Kzo9I GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
LmnI GCTCC 5 cut(s) 46, 190, 304, 391, 1102
LpnPI CCDG 3 cut(s) 756, 930, 990
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 3 cut(s) 44, 75, 535
MaeI CTAG 1 cut(s) 663
MaeII ACGT 1 cut(s) 224
MalI GATC 6 cut(s) 158, 200, 463, 503, 667, 1056
MboI GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
MboII GAAGA 3 cut(s) 507, 620, 833
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 802, 973
MluCI AATT 4 cut(s) 46, 219, 454, 842
MluI ACGCGT 1 cut(s) 570
MroI TCCGGA 1 cut(s) 916
MseI TTAA 5 cut(s) 21, 69, 167, 330, 1122
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 1 cut(s) 917
Mva1269I GAATGC 2 cut(s) 262, 350
MvnI CGCG 2 cut(s) 572, 633
MwoI GCNNNNNNNGC 5 cut(s) 38, 289, 726, 977, 1103
NdeII GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
NlaIII CATG 7 cut(s) 157, 271, 358, 419, 518, 685, 829
NlaIV GGNNCC 2 cut(s) 306, 741
NspI RCATGY 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 2 cut(s) 262, 350
PfeI GAWTC 2 cut(s) 132, 172
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 306, 741
PspPI GGNCC 3 cut(s) 371, 704, 740
PspPPI RGGWCCY 1 cut(s) 371
PsuI RGATCY 1 cut(s) 501
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 5 cut(s) 21, 69, 167, 330, 1122
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 6 cut(s) 156, 198, 461, 501, 665, 1054
Sau96I GGNCC 3 cut(s) 371, 704, 740
ScaI AGTACT 1 cut(s) 164
SduI GDGCHC 2 cut(s) 802, 973
SfaNI GCATC 3 cut(s) 44, 75, 535
SinI GGWCC 2 cut(s) 371, 740
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 1065
SmoI CTYRAG 1 cut(s) 1065
Sse9I AATT 4 cut(s) 46, 219, 454, 842
SsiI CCGC 2 cut(s) 88, 633
SspMI CTAG 1 cut(s) 663
TaaI ACNGT 2 cut(s) 278, 653
TaiI ACGT 1 cut(s) 227
TaqI TCGA 3 cut(s) 191, 299, 579
TasI AATT 4 cut(s) 46, 219, 454, 842
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 2 cut(s) 132, 172
Tru1I TTAA 5 cut(s) 21, 69, 167, 330, 1122
Tru9I TTAA 5 cut(s) 21, 69, 167, 330, 1122
TscAI CASTG 2 cut(s) 283, 785
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 6 cut(s) 284, 343, 431, 670, 894, 1003
TspRI CASTG 2 cut(s) 283, 785
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 371, 740
XapI RAATTY 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 1 cut(s) 518
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
XmiI GTMKAC 2 cut(s) 648, 859
XspI CTAG 1 cut(s) 663
ZrmI AGTACT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.