Rorug02G0369700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008248
Physical Location & Seq
Reverse (-)
47136048 .. 47151571
15524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0369700.1

Sequence Viewer

Length: 2304 bp
ATGGCGGTGCTGCTCTCGTACCTTGCCACGGAGATTGTGATCCCGGTGTGCGCCGTGGTCGGGATCTTGTTCTCGCTCGCGCAGTGGTACTTGGTGTCGCGCGTGAGGGTCACGCCCGACCGCAACGCGCCGCCGGCGGGAACGAACACCAAGAAGAACGGCTTCAACGACTACCTCATTGAGGAGGAGGAAGGCGTCAACGACCAAACCGTCGTCGCTAAGTGCGCCGAGATTCAGAATGCTATCTCCGAAGGTGCTACATCCTTCCTTTTCACCGAATATCAGTATGTCGGGGTCTTCATGGTTGTTTTTGCGGTCTTGATCTTCCTCTTCCTCGGGTCCGTGAAGGGCTTCAGCACAGAGAGCCAGCCGTGCACTTATGATGCGACAAACACCTGCAAGCCAGCACTTGCTACTGCAGTCTTCAGCACTGTTGCTTTCTTACTTGGTGGTATCACCTCACTCCTCTCTGGTTTTCTTGGGATGAAAATCGCCACTTATGCCAATGCCAGGACAACATTGGAAGCAAGAAAAGGTGTGGGGAAGGCCTTTATTGTGGCCTTTAGGTCTGGCGCAGTTATGGGTTTTCTCCTTGCAGCCAATGGTCTTTTGGTCCTTTACATTACCATCAATCTCTTTCAGCTGTACTATGGTGATGACTGGGAAGGCCTTTTTGAGGCAATTACTGGCTATGGTCTTGGTGGATCAGCAATGGCTCTTTTTGGGAGAGTAGGTGGTGGTATCTATACCAAGGCTGCTGATGTTGGTGCCGACCTTGTTGGAAAAATTGAAAGAAACATTCCTGAAGATGACCCAAGAAACCCGGCTGTGATTGCTGACAATGTTGGTGATAATGTTGGGGATATTGCTGGTATGGGATCCGATCTTTTTGGCTCATATGCTGAATCATCTTGTGCTGCTCTAGTTGTTGCTTCCATTTCCTCATTTGGAATCAACCATGAGTTCACCGCTATGCTGTATCCCCTCCTCGTCAGTTCTATGGGCATTCTTGTTTGTTTAATCACAACCTTGTTTGCCACTGATTTCTTTGAGATCAAGGCGGTCAAGGACATTGAACCAGCTTTGAAAAAGCAGCTTATCATCTCCACTGTTCTTATGACTATTGGAATTGCAATTGTAAGCTGGGTTGCTCTGCCATCATCCTTCACAATTTACAATTTTGGAGTTCAGAAAGTTGAAAAGAACTGGCAATTGTTCTTGTGTGTATCTGTTGGTCTTTGGGCAGGACTTATCATTGGGTTCGTCACCGAGTATTATACTAGCAATGCATACAGCCCCGTGCAGGATGTTGCTGATTCCTGTAGGACTGGTGCTGCCACCAATGTTATCTTTGGGCTTGCTCTGGGATACAAATCAGTCATCATTCCAATTTTTGCCATTGCAGTCAGCATTTTTGTTAGTTTTAGTTTCGCTGCTATGTATGGCATTGCAGTGGCTGCTCTTGGGATGCTGAGCACCATTGCTACTGGGTTGGCAATTGATGCCTACGGGCCCATCAGTGACAATGCTGGAGGTATTGCTGAAATGGCTGGCATGAGCCATCGAATCCGTGAGAGAACTGATGCTCTTGATGCTGCTGGGAATACCACTGCTGCAATTGGAAAGGGATTTGCTATCGGATCTGCTGCTTTGGTGTCTTTGGCACTATTTGGTGCCTTTGTCAGTCGTGCAGCAATTTCAACAGTTGATGTCCTAACCCCAAAGGTCTTTATCGGATTGATCGTGGGTGCAATGCTTCCTTACTGGTTCTCTGCCATGACCATGAAGAGTGTGGGAAGTGCAGCTCTGAAAATGGTTGAGGAAGTTCGCAGGCAGTTCAATACCATCCCAGGACTCATGGAGGGTCTTGCCAAGCCCGACTATGCTACCTGTGTCAAGATCTCCACTGATGCATCCATCAAGGAGATGATTCCACCTGGTGCCCTTGTCATCCTTACACCTCTCATTGTTGGAACATTGTTTGGTGTGGAGACCCTCTCTGGTGTCCTGGCTGGTTCCCTTGTTTCTGGTGTCCAGGTAGCAATATCTGCCTCCAACACTGGTGGTGCATGGGACAATGCCAAGAAGTACATTGAGGCTGGTGCTTCGGAGCATGCAAGGACTCTTGGCCCAAAGGGGTCCGACCCACACAAGGCTGCAGTGATCGGTGACACCATTGGAGACCCACTGAAGGACACTTCAGGCCCATCACTCAACATCCTCATTAAGCTCATGGCTGTGGAGTCCCTTGTGTTTGCTCCCTTCTTTGCCACTCACGGTGGCTTGCTCTTCAAGATCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

767

Amino Acids

80.56

Weight (kDa)

5.12

Isoelectric Point (pI)

22.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 70 - 752 6.7e-255 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 3 cut(s) 767, 1673, 1940
AccII CGCG 4 cut(s) 80, 100, 102, 128
AciI CCGC 7 cut(s) 5, 121, 131, 137, 314, 969, 1061
AclWI GGATC 6 cut(s) 34, 71, 712, 873, 886, 1648
AcuI CTGAAG 5 cut(s) 337, 409, 825, 2184, 2210
AcyI GRCGYC 1 cut(s) 195
AdeI CACNNNGTG 1 cut(s) 1940
AfaI GTAC 4 cut(s) 20, 89, 647, 2090
AfiI CCNNNNNNNGG 9 cut(s) 28, 60, 137, 181, 510, 676, 1303, 2152, 2191
AgsI TTSAA 8 cut(s) 166, 791, 1076, 1087, 1199, 1701, 1840, 2293
AjnI CCWGG 5 cut(s) 509, 1849, 1936, 2007, 2034
AluBI AGCT 6 cut(s) 643, 1082, 1096, 1143, 1805, 2230
AluI AGCT 6 cut(s) 643, 1082, 1096, 1143, 1805, 2230
Alw21I GWGCWC 2 cut(s) 377, 1478
Alw26I GTCTC 2 cut(s) 1985, 2175
Alw44I GTGCAC 1 cut(s) 373
AlwI GGATC 6 cut(s) 34, 71, 712, 873, 886, 1648
AlwNI CAGNNNCTG 1 cut(s) 1457
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 6 cut(s) 546, 558, 667, 1511, 2128, 2203
ApaI GGGCCC 1 cut(s) 1515
ApaLI GTGCAC 1 cut(s) 373
Asp700I GAANNNNTTC 2 cut(s) 161, 350
AspLEI GCGC 6 cut(s) 53, 82, 102, 130, 227, 575
AspS9I GGNCC 7 cut(s) 339, 613, 1511, 1512, 2129, 2139, 2204
AsuC2I CCSGG 2 cut(s) 44, 824
AsuHPI GGTGA 7 cut(s) 265, 448, 665, 860, 958, 1258, 2180
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 3 cut(s) 339, 613, 2139
BaeGI GKGCMC 3 cut(s) 377, 1515, 1945
BamHI GGATCC 1 cut(s) 878
BanI GGYRCC 3 cut(s) 767, 1673, 1940
BanII GRGCYC 1 cut(s) 1515
BbsI GAAGAC 2 cut(s) 289, 415
Bbv12I GWGCWC 2 cut(s) 377, 1478
BccI CCATC 7 cut(s) 635, 1165, 1523, 1569, 1853, 1925, 2215
BceAI ACGGC 3 cut(s) 38, 175, 355
BciT130I CCWGG 5 cut(s) 511, 1851, 1938, 2009, 2036
BciVI GTATCC 2 cut(s) 990, 1361
BcnI CCSGG 2 cut(s) 44, 824
BcoDI GTCTC 2 cut(s) 1985, 2175
BfaI CTAG 2 cut(s) 923, 1281
BfmI CTRYAG 3 cut(s) 417, 1321, 2157
BfuAI ACCTGC 1 cut(s) 404
BfuI GTATCC 2 cut(s) 990, 1361
BglII AGATCT 2 cut(s) 1899, 2295
BlpI GCTNAGC 1 cut(s) 1472
Bme1390I CCNGG 7 cut(s) 44, 511, 824, 1851, 1938, 2009, 2036
Bme18I GGWCC 3 cut(s) 339, 613, 2139
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 7 cut(s) 339, 613, 1511, 1512, 2129, 2139, 2204
BmiI GGNNCC 8 cut(s) 340, 769, 880, 1513, 1675, 1942, 2017, 2140
BmrFI CCNGG 7 cut(s) 44, 511, 824, 1851, 1938, 2009, 2036
BmrI ACTGGG 2 cut(s) 670, 1497
BmsI GCATC 7 cut(s) 373, 1458, 1492, 1573, 1582, 1900, 1922
BmuI ACTGGG 2 cut(s) 670, 1497
BpiI GAAGAC 2 cut(s) 289, 415
BplI GAGNNNNNCTC 2 cut(s) 1982, 2014
BpmI CTGGAG 1 cut(s) 1551
Bpu1102I GCTNAGC 1 cut(s) 1472
BpuMI CCSGG 2 cut(s) 44, 824
BsaBI GATNNNNATC 3 cut(s) 38, 488, 1372
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 2 cut(s) 1985, 2175
BsaJI CCNNGG 5 cut(s) 27, 54, 334, 750, 1849
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 9 cut(s) 28, 60, 137, 181, 510, 676, 1303, 2152, 2191
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 7 cut(s) 665, 691, 1211, 1333, 1492, 1769, 2065
Bse3DI GCAATG 6 cut(s) 717, 1291, 1398, 1446, 1479, 1758
Bse8I GATNNNNATC 3 cut(s) 38, 488, 1372
BseBI CCWGG 5 cut(s) 511, 1851, 1938, 2009, 2036
BseDI CCNNGG 5 cut(s) 27, 54, 334, 750, 1849
BseGI GGATG 9 cut(s) 260, 489, 1160, 1312, 1473, 1845, 1913, 1950, 2217
BseJI GATNNNNATC 3 cut(s) 38, 488, 1372
BseLI CCNNNNNNNGG 9 cut(s) 28, 60, 137, 181, 510, 676, 1303, 2152, 2191
BseMI GCAATG 6 cut(s) 717, 1291, 1398, 1446, 1479, 1758
BseMII CTCAG 1 cut(s) 1463
BseNI ACTGG 7 cut(s) 665, 691, 1211, 1333, 1492, 1769, 2065
BseRI GAGGAG 4 cut(s) 197, 200, 455, 977
BseSI GKGCMC 3 cut(s) 377, 1515, 1945
BseYI CCCAGC 2 cut(s) 1143, 1598
BsgI GTGCAG 3 cut(s) 1322, 1710, 1821
Bsh1236I CGCG 4 cut(s) 80, 100, 102, 128
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 6 cut(s) 548, 560, 669, 1513, 2130, 2205
BshNI GGYRCC 3 cut(s) 767, 1673, 1940
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 377, 1478
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 3 cut(s) 44, 134, 824
BslFI GGGAC 2 cut(s) 2087, 2230
BslI CCNNNNNNNGG 9 cut(s) 28, 60, 137, 181, 510, 676, 1303, 2152, 2191
BsmAI GTCTC 2 cut(s) 1985, 2175
BsmFI GGGAC 2 cut(s) 2087, 2230
BsmI GAATGC 2 cut(s) 244, 1005
BsnI GGCC 6 cut(s) 548, 560, 669, 1513, 2130, 2205
Bso31I GGTCTC 2 cut(s) 1985, 2175
BsoBI CYCGRG 1 cut(s) 335
Bsp120I GGGCCC 1 cut(s) 1511
Bsp1286I GDGCHC 4 cut(s) 377, 1478, 1515, 1945
Bsp1720I GCTNAGC 1 cut(s) 1472
BspACI CCGC 7 cut(s) 5, 121, 131, 137, 314, 969, 1061
BspANI GGCC 6 cut(s) 548, 560, 669, 1513, 2130, 2205
BspCNI CTCAG 1 cut(s) 1464
BspFNI CGCG 4 cut(s) 80, 100, 102, 128
BspLI GGNNCC 8 cut(s) 340, 769, 880, 1513, 1675, 1942, 2017, 2140
BspMAI CTGCAG 2 cut(s) 421, 2161
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 6 cut(s) 34, 71, 712, 873, 886, 1648
BspQI GCTCTTC 1 cut(s) 2294
BspT107I GGYRCC 3 cut(s) 767, 1673, 1940
BspTNI GGTCTC 2 cut(s) 1985, 2175
BsrDI GCAATG 6 cut(s) 717, 1291, 1398, 1446, 1479, 1758
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 7 cut(s) 665, 691, 1211, 1333, 1492, 1769, 2065
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 5 cut(s) 27, 54, 334, 750, 1849
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 5 cut(s) 511, 1851, 1938, 2009, 2036
Bst4CI ACNGT 5 cut(s) 211, 433, 1111, 1705, 2279
Bst6I CTCTTC 3 cut(s) 335, 1781, 2294
BstACI GRCGYC 1 cut(s) 195
BstAPI GCANNNNNTGC 3 cut(s) 1457, 1502, 2048
BstDEI CTNAG 2 cut(s) 219, 1472
BstDSI CCRYGG 2 cut(s) 27, 54
BstENI CCTNNNNNAGG 2 cut(s) 179, 674
BstF5I GGATG 9 cut(s) 260, 489, 1160, 1312, 1473, 1845, 1913, 1950, 2217
BstFNI CGCG 4 cut(s) 80, 100, 102, 128
BstHHI GCGC 6 cut(s) 53, 82, 102, 130, 227, 575
BstMAI GTCTC 2 cut(s) 1985, 2175
BstMCI CGRYCG 1 cut(s) 121
BstNI CCWGG 5 cut(s) 511, 1851, 1938, 2009, 2036
BstNSI RCATGY 1 cut(s) 2117
BstSCI CCNGG 7 cut(s) 42, 509, 822, 1849, 1936, 2007, 2034
BstSFI CTRYAG 3 cut(s) 417, 1321, 2157
BstSLI GKGCMC 3 cut(s) 377, 1515, 1945
BstUI CGCG 4 cut(s) 80, 100, 102, 128
BstV2I GAAGAC 2 cut(s) 289, 415
BstX2I RGATCY 5 cut(s) 63, 878, 1640, 1899, 2295
BstYI RGATCY 5 cut(s) 63, 878, 1640, 1899, 2295
BsuI GTATCC 2 cut(s) 990, 1361
BsuRI GGCC 6 cut(s) 548, 560, 669, 1513, 2130, 2205
BtgI CCRYGG 2 cut(s) 27, 54
BtsCI GGATG 9 cut(s) 260, 489, 1160, 1312, 1473, 1845, 1913, 1950, 2217
BtsI GCAGTG 4 cut(s) 89, 1458, 1608, 2166
BveI ACCTGC 1 cut(s) 404
CaiI CAGNNNCTG 1 cut(s) 1457
CfoI GCGC 6 cut(s) 53, 82, 102, 130, 227, 575
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 7 cut(s) 339, 613, 1511, 1512, 2129, 2139, 2204
CseI GACGC 1 cut(s) 184
CsiI ACCWGGT 1 cut(s) 1936
Csp6I GTAC 4 cut(s) 19, 88, 646, 2089
CspCI CAANNNNNGTGG 4 cut(s) 16, 51, 2044, 2079
CviAII CATG 9 cut(s) 301, 959, 1555, 1777, 1783, 1858, 2070, 2114, 2233
CviQI GTAC 4 cut(s) 19, 88, 646, 2089
DdeI CTNAG 2 cut(s) 219, 1472
DraIII CACNNNGTG 1 cut(s) 1940
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 3 cut(s) 335, 1781, 2294
EarI CTCTTC 3 cut(s) 335, 1781, 2294
Eco130I CCWWGG 1 cut(s) 750
Eco147I AGGCCT 2 cut(s) 548, 669
Eco24I GRGCYC 1 cut(s) 1515
Eco31I GGTCTC 2 cut(s) 1985, 2175
Eco47I GGWCC 3 cut(s) 339, 613, 2139
Eco57I CTGAAG 5 cut(s) 337, 409, 825, 2184, 2210
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 2 cut(s) 179, 674
EcoRII CCWGG 5 cut(s) 509, 1849, 1936, 2007, 2034
EcoT14I CCWWGG 1 cut(s) 750
EcoT22I ATGCAT 2 cut(s) 1291, 1915
EcoT38I GRGCYC 1 cut(s) 1515
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 9 cut(s) 304, 962, 1558, 1780, 1786, 1861, 2073, 2117, 2236
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 2 cut(s) 2087, 2230
FatI CATG 9 cut(s) 300, 958, 1554, 1776, 1782, 1857, 2069, 2113, 2232
FauI CCCGC 1 cut(s) 130
FauNDI CATATG 1 cut(s) 898
FokI GGATG 9 cut(s) 247, 496, 1147, 1319, 1480, 1832, 1900, 1937, 2204
FriOI GRGCYC 1 cut(s) 1515
FspBI CTAG 2 cut(s) 923, 1281
GlaI GCGC 6 cut(s) 52, 81, 101, 129, 226, 574
GsaI CCCAGC 2 cut(s) 1147, 1602
GsuI CTGGAG 1 cut(s) 1551
HaeIII GGCC 6 cut(s) 548, 560, 669, 1513, 2130, 2205
HapII CCGG 3 cut(s) 44, 134, 824
HgaI GACGC 1 cut(s) 184
HhaI GCGC 6 cut(s) 53, 82, 102, 130, 227, 575
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 9 cut(s) 304, 962, 1558, 1780, 1786, 1861, 2073, 2117, 2236
Hin6I GCGC 6 cut(s) 51, 80, 100, 128, 225, 573
HinP1I GCGC 6 cut(s) 51, 80, 100, 128, 225, 573
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 9 cut(s) 232, 905, 951, 1316, 1566, 1854, 1930, 2122, 2243
HpaII CCGG 3 cut(s) 44, 134, 824
HphI GGTGA 7 cut(s) 265, 448, 665, 860, 958, 1258, 2180
Hpy166II GTNNAC 3 cut(s) 199, 375, 966
Hpy188I TCNGA 9 cut(s) 237, 250, 883, 1191, 1640, 1736, 1809, 2110, 2143
Hpy188III TCNNGA 6 cut(s) 61, 319, 803, 1589, 1897, 2293
Hpy8I GTNNAC 3 cut(s) 199, 375, 966
Hpy99I CGWCG 2 cut(s) 215, 218
HpyAV CCTTC 9 cut(s) 185, 245, 274, 340, 538, 659, 1174, 2185, 2272
HpyCH4III ACNGT 5 cut(s) 211, 433, 1111, 1705, 2279
HpyF3I CTNAG 2 cut(s) 219, 1472
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 9 cut(s) 304, 962, 1558, 1780, 1786, 1861, 2073, 2117, 2236
HspAI GCGC 6 cut(s) 51, 80, 100, 128, 225, 573
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
LguI GCTCTTC 1 cut(s) 2294
LmnI GCTCC 2 cut(s) 2110, 2263
LweI GCATC 7 cut(s) 373, 1458, 1492, 1573, 1582, 1900, 1922
MabI ACCWGGT 1 cut(s) 1936
MaeI CTAG 2 cut(s) 923, 1281
MaeIII GTNAC 4 cut(s) 109, 1264, 1520, 2168
MboII GAAGA 9 cut(s) 166, 289, 316, 322, 415, 818, 1798, 2281, 2290
MfeI CAATTG 4 cut(s) 1134, 1211, 1497, 1617
MflI RGATCY 5 cut(s) 63, 878, 1640, 1899, 2295
MhlI GDGCHC 4 cut(s) 377, 1478, 1515, 1945
MlyI GAGTC 3 cut(s) 1848, 2116, 2252
MmeI TCCRAC 4 cut(s) 760, 1951, 2079, 2166
Mph1103I ATGCAT 2 cut(s) 1291, 1915
MreI CGCCGGCG 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 133
MroXI GAANNNNTTC 2 cut(s) 161, 350
MseI TTAA 2 cut(s) 1019, 2226
MslI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 3 cut(s) 44, 134, 824
MspR9I CCNGG 7 cut(s) 44, 511, 824, 1851, 1938, 2009, 2036
MunI CAATTG 4 cut(s) 1134, 1211, 1497, 1617
Mva1269I GAATGC 2 cut(s) 244, 1005
MvaI CCWGG 5 cut(s) 511, 1851, 1938, 2009, 2036
MvnI CGCG 4 cut(s) 80, 100, 102, 128
NaeI GCCGGC 1 cut(s) 135
NciI CCSGG 2 cut(s) 44, 824
NdeI CATATG 1 cut(s) 898
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 9 cut(s) 304, 962, 1558, 1780, 1786, 1861, 2073, 2117, 2236
NlaIV GGNNCC 8 cut(s) 340, 769, 880, 1513, 1675, 1942, 2017, 2140
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 4 cut(s) 109, 1264, 1520, 2168
NsiI ATGCAT 2 cut(s) 1291, 1915
NspI RCATGY 1 cut(s) 2117
PaeI GCATGC 1 cut(s) 2117
PaqCI CACCTGC 1 cut(s) 404
PceI AGGCCT 2 cut(s) 548, 669
PciSI GCTCTTC 1 cut(s) 2294
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 207, 1740
PctI GAATGC 2 cut(s) 244, 1005
PdiI GCCGGC 1 cut(s) 135
PdmI GAANNNNTTC 2 cut(s) 161, 350
PfeI GAWTC 6 cut(s) 232, 905, 951, 1316, 1566, 1930
PleI GAGTC 3 cut(s) 1848, 2116, 2251
PpsI GAGTC 3 cut(s) 1848, 2116, 2251
Psp6I CCWGG 5 cut(s) 509, 1849, 1936, 2007, 2034
PspFI CCCAGC 2 cut(s) 1143, 1598
PspGI CCWGG 5 cut(s) 509, 1849, 1936, 2007, 2034
PspN4I GGNNCC 8 cut(s) 340, 769, 880, 1513, 1675, 1942, 2017, 2140
PspOMI GGGCCC 1 cut(s) 1511
PspPI GGNCC 7 cut(s) 339, 613, 1511, 1512, 2129, 2139, 2204
PstI CTGCAG 2 cut(s) 421, 2161
PstNI CAGNNNCTG 1 cut(s) 1457
PsuI RGATCY 5 cut(s) 63, 878, 1640, 1899, 2295
PvuII CAGCTG 1 cut(s) 643
RsaI GTAC 4 cut(s) 20, 89, 647, 2090
RsaNI GTAC 4 cut(s) 19, 88, 646, 2089
RseI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
SapI GCTCTTC 1 cut(s) 2294
SaqAI TTAA 2 cut(s) 1019, 2226
Sau96I GGNCC 7 cut(s) 339, 613, 1511, 1512, 2129, 2139, 2204
SchI GAGTC 3 cut(s) 1848, 2116, 2252
ScrFI CCNGG 7 cut(s) 44, 511, 824, 1851, 1938, 2009, 2036
SduI GDGCHC 4 cut(s) 377, 1478, 1515, 1945
SexAI ACCWGGT 1 cut(s) 1936
SfaNI GCATC 7 cut(s) 373, 1458, 1492, 1573, 1582, 1900, 1922
SfcI CTRYAG 3 cut(s) 417, 1321, 2157
SgrAI CRCCGGYG 1 cut(s) 133
SinI GGWCC 3 cut(s) 339, 613, 2139
SmiMI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
SphI GCATGC 1 cut(s) 2117
SseBI AGGCCT 2 cut(s) 548, 669
SsiI CCGC 7 cut(s) 5, 121, 131, 137, 314, 969, 1061
SspMI CTAG 2 cut(s) 923, 1281
StuI AGGCCT 2 cut(s) 548, 669
StyD4I CCNGG 7 cut(s) 42, 509, 822, 1849, 1936, 2007, 2034
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 5 cut(s) 211, 433, 1111, 1705, 2279
TaqI TCGA 1 cut(s) 1564
TatI WGTACW 2 cut(s) 645, 2088
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 6 cut(s) 232, 905, 951, 1316, 1566, 1930
Tru1I TTAA 2 cut(s) 1019, 2226
Tru9I TTAA 2 cut(s) 1019, 2226
TseFI GTSAC 4 cut(s) 109, 1264, 1520, 2168
Tsp45I GTSAC 4 cut(s) 109, 1264, 1520, 2168
TspDTI ATGAA 3 cut(s) 289, 500, 1799
TspGWI ACGGA 3 cut(s) 44, 331, 1559
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 3 cut(s) 339, 613, 2139
XagI CCTNNNNNAGG 2 cut(s) 179, 674
XceI RCATGY 1 cut(s) 2117
XcmI CCANNNNNNNNNTGG 3 cut(s) 517, 607, 1789
XmnI GAANNNNTTC 2 cut(s) 161, 350
XspI CTAG 2 cut(s) 923, 1281
Zsp2I ATGCAT 2 cut(s) 1291, 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.