FvH4_6g32820

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008248
Physical Location & Seq
Forward (+)
25913468 .. 25917223
3756 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32820.t3

Sequence Viewer

Length: 1152 bp
ATGCAGTCAAGGGTGGGGGGATTGGAGGAAGGCAAGGACCCTGGTGGTAGTTTATTAAAAACCAGGGCCTTGCCATTCAGGCTGATCCAATTCCTGTTAATGTTTGTGGTTCTGGGTCTCGGGGTTTCGATTTTGAGTATGCATACAATCCGGTATTTTGGAGTTCAACATATGGCTCCAGCAGAACCATCTACTATGAGGTCATGTTTTGCAGAGCCAAACACATTAGAGAGCTGGATTAGACCTCCATCGAGTCTGTTGCATAACATGAATGACACTGAGTTGCTGTGGCTGGCCTCAATGGCTCCTCAAGTAAAGGAATATCCGTTCAAAAGAGTTCCCAAGATTGCTTTCATGTTTTTGACCAAGGGACCTTTGCCGATGGAGCCTCTTTGGGAGCGGTTTTTTAAAGGGCACGAGGGGCTTTACTCGATCTATGTCCATTCATTGCCGTCTTATACTCCCAACTTCTCAGCTACGTCAGTGTTTTACAAGAGACAAATCCCAAGCAAGCTCGCAGAGTGGGGAGAGATGAACATGTGTGAAGCTGAGAGAAGACTCCTAGCTAATGCATTGCTTGACATCTCAAATGAATGGTTTATTCTCCTTTCAGAGTCATGTATTCCTCTGAGCAACCTCAGCATTGTCTACCATTACTTATCGAGATCAAGGTATAGCTTTATGGGTTCATTTGACGAGATTGGACCTTATGGGAGAGGACGCTATAATGAACACATGGCCCCTTTGGTCAATCTCAGTAATTGGCGTAAAGGGTCCCAGTGGTTTGAGATCAACCGGGAACTTGCACATATGATTGTTCAAGACGTAACTTACTACCCTGTATTCAGAGACTACTGCAAACCTGCATGTTATGTAGATGAGCACTACTTTCAGACGATGCTTACCATCGAGACTCCATATCTTTTGGCAAATAGGACTCTTACTTATGTTGACTGGTCAAGAGGTGGTGCTCATCCAGCTACATTCGGTAAAAATGATATTACAGAAGAGTTCTTTAAGAAGATTACTACAAGCGAACAATGTCTCTACAACAACCAGCCGACCTCGCTCTGTTTCCTTTTTGCTAGAAAGTTTGCTCCAAGCGCTTTGGAGCCTCTGTTAGAATTAGCATCTAAAGTTTTTGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

384

Amino Acids

44.15

Weight (kDa)

7.63

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 116 - 342 4.6e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 871
AccBSI CCGCTC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 648
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 79
AfeI AGCGCT 1 cut(s) 1105
AflIII ACRYGT 1 cut(s) 537
AgsI TTSAA 3 cut(s) 167, 331, 821
AjnI CCWGG 2 cut(s) 40, 62
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
AluBI AGCT 7 cut(s) 234, 476, 514, 548, 566, 678, 980
AluI AGCT 7 cut(s) 234, 476, 514, 548, 566, 678, 980
Alw21I GWGCWC 2 cut(s) 885, 973
Alw26I GTCTC 5 cut(s) 122, 490, 843, 905, 1049
AlwI GGATC 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 119
Aor51HI AGCGCT 1 cut(s) 1105
AoxI GGCC 3 cut(s) 66, 294, 738
AspLEI GCGC 1 cut(s) 1106
AspS9I GGNCC 6 cut(s) 37, 66, 371, 704, 739, 774
AsuC2I CCSGG 1 cut(s) 797
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 4 cut(s) 37, 371, 704, 774
BaeGI GKGCMC 1 cut(s) 417
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 2 cut(s) 885, 973
BbvCI CCTCAGC 1 cut(s) 638
BccI CCATC 4 cut(s) 196, 256, 376, 914
BceAI ACGGC 1 cut(s) 436
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 2 cut(s) 42, 64
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 5 cut(s) 122, 490, 843, 905, 1049
BfaI CTAG 2 cut(s) 563, 1086
BfoI RGCGCY 1 cut(s) 1107
BfuAI ACCTGC 1 cut(s) 871
BglI GCCNNNNNGGC 2 cut(s) 79, 302
Bme1390I CCNGG 3 cut(s) 42, 64, 797
Bme18I GGWCC 4 cut(s) 37, 371, 704, 774
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 6 cut(s) 37, 66, 371, 704, 739, 774
BmiI GGNNCC 9 cut(s) 39, 177, 306, 372, 387, 741, 775, 776, 1113
BmrFI CCNGG 3 cut(s) 42, 64, 797
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 2 cut(s) 888, 1139
BmuI ACTGGG 1 cut(s) 772
BpiI GAAGAC 1 cut(s) 562
BpmI CTGGAG 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 797
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 40, 63, 366
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 1103, 1133
Bse1I ACTGG 2 cut(s) 778, 959
Bse3DI GCAATG 2 cut(s) 446, 572
BseBI CCWGG 2 cut(s) 42, 64
BseDI CCNNGG 3 cut(s) 40, 63, 366
BseGI GGATG 1 cut(s) 973
BseMI GCAATG 2 cut(s) 446, 572
BseMII CTCAG 6 cut(s) 270, 486, 540, 620, 652, 769
BseNI ACTGG 2 cut(s) 778, 959
BseRI GAGGAG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 417
BshFI GGCC 3 cut(s) 68, 296, 740
BsiHKAI GWGCWC 2 cut(s) 885, 973
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 151, 796
BslFI GGGAC 2 cut(s) 384, 760
BsmAI GTCTC 5 cut(s) 122, 490, 843, 905, 1049
BsmFI GGGAC 2 cut(s) 384, 760
BsnI GGCC 3 cut(s) 68, 296, 740
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 417, 885, 973
Bsp143I GATC 4 cut(s) 84, 432, 665, 789
BspACI CCGC 1 cut(s) 400
BspANI GGCC 3 cut(s) 68, 296, 740
BspCNI CTCAG 6 cut(s) 271, 485, 541, 621, 651, 768
BspLI GGNNCC 9 cut(s) 39, 177, 306, 372, 387, 741, 775, 776, 1113
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 1 cut(s) 79
BspTNI GGTCTC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 446, 572
BsrI ACTGG 2 cut(s) 778, 959
BssECI CCNNGG 3 cut(s) 40, 63, 366
BssMI GATC 4 cut(s) 84, 432, 665, 789
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 42, 64
Bst6I CTCTTC 1 cut(s) 1002
BstC8I GCNNGC 3 cut(s) 294, 512, 516
BstDEI CTNAG 6 cut(s) 279, 472, 549, 629, 638, 755
BstF5I GGATG 1 cut(s) 973
BstH2I RGCGCY 1 cut(s) 1107
BstHHI GCGC 1 cut(s) 1106
BstKTI GATC 4 cut(s) 87, 435, 668, 792
BstMAI GTCTC 5 cut(s) 122, 490, 843, 905, 1049
BstMBI GATC 4 cut(s) 84, 432, 665, 789
BstMWI GCNNNNNNNGC 8 cut(s) 79, 302, 385, 421, 639, 977, 1066, 1103
BstNI CCWGG 2 cut(s) 42, 64
BstNSI RCATGY 2 cut(s) 541, 870
BstSCI CCNGG 3 cut(s) 40, 62, 795
BstSLI GKGCMC 1 cut(s) 417
BstV2I GAAGAC 1 cut(s) 562
BsuRI GGCC 3 cut(s) 68, 296, 740
BtsCI GGATG 1 cut(s) 973
BtsIMutI CAGTG 3 cut(s) 276, 489, 785
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 3 cut(s) 294, 512, 516
CfoI GCGC 1 cut(s) 1106
Cfr13I GGNCC 6 cut(s) 37, 66, 371, 704, 739, 774
CseI GACGC 1 cut(s) 729
CviAII CATG 7 cut(s) 204, 268, 355, 538, 618, 736, 867
DdeI CTNAG 6 cut(s) 279, 472, 549, 629, 638, 755
DpnI GATC 4 cut(s) 86, 434, 667, 791
DpnII GATC 4 cut(s) 84, 432, 665, 789
DraI TTTAAA 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
Eco130I CCWWGG 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 4 cut(s) 37, 371, 704, 774
Eco47III AGCGCT 1 cut(s) 1105
Eco88I CYCGRG 1 cut(s) 119
EcoO109I RGGNCCY 4 cut(s) 37, 66, 371, 774
EcoRII CCWGG 2 cut(s) 40, 62
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 2 cut(s) 144, 574
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 7 cut(s) 207, 271, 358, 541, 621, 739, 870
FaqI GGGAC 2 cut(s) 384, 760
FatI CATG 7 cut(s) 203, 267, 354, 537, 617, 735, 866
FauNDI CATATG 2 cut(s) 171, 810
FblI GTMKAC 1 cut(s) 648
FokI GGATG 1 cut(s) 960
FspBI CTAG 2 cut(s) 563, 1086
GlaI GCGC 1 cut(s) 1105
GsuI CTGGAG 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 1107
HaeIII GGCC 3 cut(s) 68, 296, 740
HapII CCGG 2 cut(s) 151, 796
HgaI GACGC 1 cut(s) 729
HhaI GCGC 1 cut(s) 1106
Hin1II CATG 7 cut(s) 207, 271, 358, 541, 621, 739, 870
Hin6I GCGC 1 cut(s) 1104
HinP1I GCGC 1 cut(s) 1104
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 5 cut(s) 253, 558, 614, 913, 937
HpaII CCGG 2 cut(s) 151, 796
Hpy166II GTNNAC 2 cut(s) 649, 952
Hpy188I TCNGA 4 cut(s) 613, 630, 848, 894
Hpy188III TCNNGA 4 cut(s) 663, 821, 910, 960
Hpy8I GTNNAC 2 cut(s) 649, 952
HpyAV CCTTC 1 cut(s) 23
HpyCH4IV ACGT 2 cut(s) 479, 825
HpyCH4V TGCA 8 cut(s) 4, 142, 212, 262, 572, 806, 858, 866
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 302, 385, 421, 639, 977, 1066, 1103
HpyF3I CTNAG 6 cut(s) 279, 472, 549, 629, 638, 755
HpySE526I ACGT 2 cut(s) 479, 825
Hsp92II CATG 7 cut(s) 207, 271, 358, 541, 621, 739, 870
HspAI GCGC 1 cut(s) 1104
KflI GGGWCCC 1 cut(s) 774
Kzo9I GATC 4 cut(s) 84, 432, 665, 789
LmnI GCTCC 6 cut(s) 181, 310, 385, 397, 1102, 1111
LweI GCATC 2 cut(s) 888, 1139
MaeI CTAG 2 cut(s) 563, 1086
MaeII ACGT 2 cut(s) 479, 825
MaeIII GTNAC 1 cut(s) 826
MalI GATC 4 cut(s) 86, 434, 667, 791
MbiI CCGCTC 1 cut(s) 400
MboI GATC 4 cut(s) 84, 432, 665, 789
MboII GAAGA 3 cut(s) 567, 1019, 1033
MhlI GDGCHC 3 cut(s) 417, 885, 973
MluCI AATT 3 cut(s) 89, 760, 1124
MlyI GAGTC 5 cut(s) 262, 552, 623, 907, 931
Mph1103I ATGCAT 2 cut(s) 144, 574
MseI TTAA 4 cut(s) 56, 98, 408, 1017
MspI CCGG 2 cut(s) 151, 796
MspR9I CCNGG 3 cut(s) 42, 64, 797
MvaI CCWGG 2 cut(s) 42, 64
MwoI GCNNNNNNNGC 8 cut(s) 79, 302, 385, 421, 639, 977, 1066, 1103
NciI CCSGG 1 cut(s) 797
NdeI CATATG 2 cut(s) 171, 810
NdeII GATC 4 cut(s) 84, 432, 665, 789
NlaIII CATG 7 cut(s) 207, 271, 358, 541, 621, 739, 870
NlaIV GGNNCC 9 cut(s) 39, 177, 306, 372, 387, 741, 775, 776, 1113
NsiI ATGCAT 2 cut(s) 144, 574
NspI RCATGY 2 cut(s) 541, 870
PciI ACATGT 1 cut(s) 537
PleI GAGTC 5 cut(s) 261, 552, 622, 907, 931
PpsI GAGTC 5 cut(s) 261, 552, 622, 907, 931
PpuMI RGGWCCY 3 cut(s) 37, 371, 774
PscI ACATGT 1 cut(s) 537
Psp5II RGGWCCY 3 cut(s) 37, 371, 774
Psp6I CCWGG 2 cut(s) 40, 62
PspGI CCWGG 2 cut(s) 40, 62
PspN4I GGNNCC 9 cut(s) 39, 177, 306, 372, 387, 741, 775, 776, 1113
PspPI GGNCC 6 cut(s) 37, 66, 371, 704, 739, 774
PspPPI RGGWCCY 3 cut(s) 37, 371, 774
SaqAI TTAA 4 cut(s) 56, 98, 408, 1017
Sau3AI GATC 4 cut(s) 84, 432, 665, 789
Sau96I GGNCC 6 cut(s) 37, 66, 371, 704, 739, 774
SchI GAGTC 5 cut(s) 262, 552, 623, 907, 931
ScrFI CCNGG 3 cut(s) 42, 64, 797
SduI GDGCHC 3 cut(s) 417, 885, 973
SfaNI GCATC 2 cut(s) 888, 1139
SinI GGWCC 4 cut(s) 37, 371, 704, 774
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
Sse9I AATT 3 cut(s) 89, 760, 1124
SsiI CCGC 1 cut(s) 400
SspMI CTAG 2 cut(s) 563, 1086
StyD4I CCNGG 3 cut(s) 40, 62, 795
StyI CCWWGG 1 cut(s) 366
TaiI ACGT 2 cut(s) 482, 828
TaqI TCGA 5 cut(s) 128, 251, 431, 662, 909
TasI AATT 3 cut(s) 89, 760, 1124
Tru1I TTAA 4 cut(s) 56, 98, 408, 1017
Tru9I TTAA 4 cut(s) 56, 98, 408, 1017
TscAI CASTG 3 cut(s) 283, 489, 785
TspDTI ATGAA 7 cut(s) 284, 343, 435, 548, 606, 678, 744
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 3 cut(s) 283, 489, 785
VpaK11BI GGWCC 4 cut(s) 37, 371, 704, 774
XceI RCATGY 2 cut(s) 541, 870
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 2 cut(s) 563, 1086
Zsp2I ATGCAT 2 cut(s) 144, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.