pycom09g11460

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008248
Physical Location & Seq
Reverse (-)
9667974 .. 9669525
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11460.1

Sequence Viewer

Length: 1158 bp
ATGCAGTCAAGGGGAGTGGTGGCATTGGAGGAAGGCAAGGATCCTGCTACTACATTTAAATCCAGCCATGCCAGGGCCTTGCCGTTGCGACTCGTTCAATTTCTTGTCATGTTTGTAGTTTTGGGTCTTGGGTTTTCGATGTTGAGTATGCACTCCATTCGGTTTTTCCCTGTTCAGCATGTGGCTCCAACAGCACCTTCCACGATTAGGCCTTGCTTCGAAGAGCCGAGTAGTTTAGAAAGTCGGATTAGACCTCCATCTAATCTGTTGCACGCCATGAATGACACCGAGTTGCTGTGGCTCGCTTCACATGCTTCTCAAATAAAGGAGTATCCGTTTAAGAGAGTTCCTAAAATTGCCTTCATGTTCTTAACAAAGGGACCATTGCCGATGGAGCCTCTTTGGGAGCGGTTTTTAAGAGGACACGAGGGACTTTATTCGATCTATGTGCATTCGTTGCCATCTTATAATGCCAATTTCTCACCTACATCAGTGTTTTACAAGAGACAAATCCCAAGCCAGGTTGCCGAGTGGGGAGAGATGAGTATGTGCGAAGCTGAGAGGAGACTCCTAGCTAATGCATTGCTTGACATCTCAAATGAATGGTTTATCCTCCTTTCCGAGTCATGTATTCCTCTCTACAACTTGAGCATTGTCTATCACTACCTATCAAGATCCAGGTACAGCTTCATGGGTTCGTTTGATGAAAATGGGCCCTATGGGAGAGGACGTTATGACGGACGCATGGCCCCTTTGGTCAATCTCTCTGATTGGCGTAAAGGGTCTCAGTGGTTTGAGATTAAACGGGAACTTGCAGTTAGGATTGTTGGAGACACAACTTACTACCCTAAGTTCAAGGACTTTTGCAAACCAGCCTGTTATGTGGATGAGCACTACTTCCAGACAATGCTTAGCATCGAGACTCCGCATCTTTTGGCAAACAGGACCCTTACCTATGTAGACTGGTCTAGGGGCGGTGCTCATCCGGCTACATTCGGCAAAGCTGATATTACAGAGGAGTTTTTCAAGAGGATTGGAAGTGATACATGTCTTTACAACAACCAGCCAACCTCACTCTGTTTCCTTTTTGCACGAAAGTTTGCCCCGAGTGCTTTGGAACCCCTGTTAGAATTAGCATCGAAAGTATTTGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

386

Amino Acids

44.13

Weight (kDa)

8.56

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 119 - 345 7.1e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 960
AciI CCGC 3 cut(s) 409, 926, 975
AclWI GGATC 3 cut(s) 35, 48, 669
AfaI GTAC 1 cut(s) 683
AfiI CCNNNNNNNGG 3 cut(s) 73, 207, 520
AflIII ACRYGT 1 cut(s) 1046
AgsI TTSAA 3 cut(s) 98, 856, 1027
AjnI CCWGG 3 cut(s) 71, 519, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 181, 213
AluBI AGCT 4 cut(s) 557, 575, 687, 1004
AluI AGCT 4 cut(s) 557, 575, 687, 1004
Alw21I GWGCWC 2 cut(s) 894, 982
Alw26I GTCTC 5 cut(s) 499, 559, 789, 825, 914
AlwI GGATC 3 cut(s) 35, 48, 669
Ama87I CYCGRG 1 cut(s) 1105
AoxI GGCC 4 cut(s) 75, 209, 713, 747
ApaI GGGCCC 1 cut(s) 717
AspS9I GGNCC 6 cut(s) 75, 380, 713, 714, 748, 945
AsuHPI GGTGA 1 cut(s) 474
AsuII TTCGAA 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 1105
AvaII GGWCC 2 cut(s) 380, 945
BaeGI GKGCMC 1 cut(s) 717
BamHI GGATCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 717
BauI CACGAG 1 cut(s) 425
Bbv12I GWGCWC 2 cut(s) 894, 982
BccI CCATC 3 cut(s) 265, 385, 469
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 430, 464
BciT130I CCWGG 3 cut(s) 73, 521, 679
BciVI GTATCC 1 cut(s) 342
BcoDI GTCTC 5 cut(s) 499, 559, 789, 825, 914
BfaI CTAG 2 cut(s) 572, 969
BfuI GTATCC 1 cut(s) 342
BlpI GCTNAGC 1 cut(s) 911
Bme1390I CCNGG 3 cut(s) 73, 521, 679
Bme18I GGWCC 2 cut(s) 380, 945
BmeT110I CYCGRG 1 cut(s) 1105
BmgT120I GGNCC 6 cut(s) 75, 380, 713, 714, 748, 945
BmiI GGNNCC 8 cut(s) 42, 186, 381, 396, 715, 750, 947, 1119
BmrFI CCNGG 3 cut(s) 73, 521, 679
BmsI GCATC 3 cut(s) 924, 937, 1145
Bpu1102I GCTNAGC 1 cut(s) 911
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 789
BsaJI CCNNGG 1 cut(s) 72
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 715, 745
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 207, 520
Bse1I ACTGG 1 cut(s) 968
Bse3DI GCAATG 2 cut(s) 383, 581
BseBI CCWGG 3 cut(s) 73, 521, 679
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 2 cut(s) 892, 982
BseLI CCNNNNNNNGG 3 cut(s) 73, 207, 520
BseMI GCAATG 2 cut(s) 383, 581
BseMII CTCAG 2 cut(s) 549, 800
BseNI ACTGG 1 cut(s) 968
BseRI GAGGAG 2 cut(s) 577, 1031
BseSI GKGCMC 1 cut(s) 717
BshFI GGCC 4 cut(s) 77, 211, 715, 749
BsiHKAI GWGCWC 2 cut(s) 894, 982
BsiHKCI CYCGRG 1 cut(s) 1105
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 2 cut(s) 393, 444
BslI CCNNNNNNNGG 3 cut(s) 73, 207, 520
BsmAI GTCTC 5 cut(s) 499, 559, 789, 825, 914
BsmFI GGGAC 2 cut(s) 393, 444
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 4 cut(s) 77, 211, 715, 749
Bso31I GGTCTC 1 cut(s) 789
BsoBI CYCGRG 1 cut(s) 1105
Bsp119I TTCGAA 1 cut(s) 219
Bsp120I GGGCCC 1 cut(s) 713
Bsp1286I GDGCHC 3 cut(s) 717, 894, 982
Bsp143I GATC 3 cut(s) 40, 441, 674
Bsp1720I GCTNAGC 1 cut(s) 911
BspACI CCGC 3 cut(s) 409, 926, 975
BspANI GGCC 4 cut(s) 77, 211, 715, 749
BspCNI CTCAG 2 cut(s) 550, 799
BspLI GGNNCC 8 cut(s) 42, 186, 381, 396, 715, 750, 947, 1119
BspPI GGATC 3 cut(s) 35, 48, 669
BspQI GCTCTTC 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 789
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 383, 581
BsrI ACTGG 1 cut(s) 968
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 3 cut(s) 40, 441, 674
BssSI CACGAG 1 cut(s) 425
Bst2BI CACGAG 1 cut(s) 425
Bst2UI CCWGG 3 cut(s) 73, 521, 679
Bst6I CTCTTC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 273, 303
BstDEI CTNAG 4 cut(s) 558, 786, 849, 911
BstF5I GGATG 2 cut(s) 892, 982
BstKTI GATC 3 cut(s) 43, 444, 677
BstMAI GTCTC 5 cut(s) 499, 559, 789, 825, 914
BstMBI GATC 3 cut(s) 40, 441, 674
BstMWI GCNNNNNNNGC 6 cut(s) 191, 311, 394, 457, 986, 1109
BstNI CCWGG 3 cut(s) 73, 521, 679
BstNSI RCATGY 3 cut(s) 182, 314, 1050
BstSCI CCNGG 3 cut(s) 71, 519, 677
BstSLI GKGCMC 1 cut(s) 717
BstX2I RGATCY 2 cut(s) 40, 674
BstYI RGATCY 2 cut(s) 40, 674
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 4 cut(s) 77, 211, 715, 749
BtsCI GGATG 2 cut(s) 892, 982
BtsIMutI CAGTG 2 cut(s) 498, 794
Cac8I GCNNGC 2 cut(s) 273, 303
Cfr13I GGNCC 6 cut(s) 75, 380, 713, 714, 748, 945
CseI GACGC 1 cut(s) 750
Csp6I GTAC 1 cut(s) 682
CspCI CAANNNNNGTGG 1 cut(s) 32
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 4 cut(s) 558, 786, 849, 911
DpnI GATC 3 cut(s) 42, 443, 676
DpnII GATC 3 cut(s) 40, 441, 674
DraI TTTAAA 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 1 cut(s) 789
Eco47I GGWCC 2 cut(s) 380, 945
Eco88I CYCGRG 1 cut(s) 1105
EcoO109I RGGNCCY 3 cut(s) 75, 714, 945
EcoRII CCWGG 3 cut(s) 71, 519, 677
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 717
FaqI GGGAC 2 cut(s) 393, 444
FblI GTMKAC 1 cut(s) 960
FokI GGATG 2 cut(s) 899, 969
FriOI GRGCYC 1 cut(s) 717
FspBI CTAG 2 cut(s) 572, 969
HaeIII GGCC 4 cut(s) 77, 211, 715, 749
HapII CCGG 1 cut(s) 986
HgaI GACGC 1 cut(s) 750
HinfI GANTC 4 cut(s) 90, 567, 623, 922
HpaII CCGG 1 cut(s) 986
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 1 cut(s) 961
Hpy188I TCNGA 3 cut(s) 246, 622, 769
Hpy188III TCNNGA 4 cut(s) 672, 901, 919, 1027
Hpy8I GTNNAC 1 cut(s) 961
HpyAV CCTTC 3 cut(s) 26, 207, 370
HpyCH4IV ACGT 1 cut(s) 730
HpyCH4V TGCA 8 cut(s) 4, 151, 271, 451, 581, 815, 867, 1091
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 311, 394, 457, 986, 1109
HpyF3I CTNAG 4 cut(s) 558, 786, 849, 911
HpySE526I ACGT 1 cut(s) 730
Kzo9I GATC 3 cut(s) 40, 441, 674
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 3 cut(s) 190, 394, 406
LweI GCATC 3 cut(s) 924, 937, 1145
MaeI CTAG 2 cut(s) 572, 969
MaeII ACGT 1 cut(s) 730
MalI GATC 3 cut(s) 42, 443, 676
MbiI CCGCTC 1 cut(s) 409
MboI GATC 3 cut(s) 40, 441, 674
MboII GAAGA 1 cut(s) 233
MflI RGATCY 2 cut(s) 40, 674
MhlI GDGCHC 3 cut(s) 717, 894, 982
MluCI AATT 4 cut(s) 98, 354, 475, 1130
MlyI GAGTC 4 cut(s) 84, 561, 632, 916
MmeI TCCRAC 3 cut(s) 212, 224, 808
Mph1103I ATGCAT 1 cut(s) 583
MseI TTAA 5 cut(s) 57, 339, 371, 416, 801
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 3 cut(s) 73, 521, 679
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 3 cut(s) 73, 521, 679
MwoI GCNNNNNNNGC 6 cut(s) 191, 311, 394, 457, 986, 1109
NdeII GATC 3 cut(s) 40, 441, 674
NlaIV GGNNCC 8 cut(s) 42, 186, 381, 396, 715, 750, 947, 1119
NmeAIII GCCGAG 2 cut(s) 252, 553
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 3 cut(s) 182, 314, 1050
NspV TTCGAA 1 cut(s) 219
PceI AGGCCT 1 cut(s) 211
PciI ACATGT 1 cut(s) 1046
PciSI GCTCTTC 1 cut(s) 216
PctI GAATGC 1 cut(s) 451
PleI GAGTC 4 cut(s) 84, 561, 631, 916
PpsI GAGTC 4 cut(s) 84, 561, 631, 916
PpuMI RGGWCCY 1 cut(s) 945
PscI ACATGT 1 cut(s) 1046
PsiI TTATAA 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 945
Psp6I CCWGG 3 cut(s) 71, 519, 677
PspGI CCWGG 3 cut(s) 71, 519, 677
PspN4I GGNNCC 8 cut(s) 42, 186, 381, 396, 715, 750, 947, 1119
PspOMI GGGCCC 1 cut(s) 713
PspPI GGNCC 6 cut(s) 75, 380, 713, 714, 748, 945
PspPPI RGGWCCY 1 cut(s) 945
PsuI RGATCY 2 cut(s) 40, 674
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 5 cut(s) 57, 339, 371, 416, 801
Sau3AI GATC 3 cut(s) 40, 441, 674
Sau96I GGNCC 6 cut(s) 75, 380, 713, 714, 748, 945
SchI GAGTC 4 cut(s) 84, 561, 632, 916
ScrFI CCNGG 3 cut(s) 73, 521, 679
SduI GDGCHC 3 cut(s) 717, 894, 982
SfaNI GCATC 3 cut(s) 924, 937, 1145
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 2 cut(s) 380, 945
SmiI ATTTAAAT 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 4 cut(s) 98, 354, 475, 1130
SseBI AGGCCT 1 cut(s) 211
SsiI CCGC 3 cut(s) 409, 926, 975
SspMI CTAG 2 cut(s) 572, 969
StuI AGGCCT 1 cut(s) 211
StyD4I CCNGG 3 cut(s) 71, 519, 677
SwaI ATTTAAAT 1 cut(s) 58
TaiI ACGT 1 cut(s) 733
TaqI TCGA 5 cut(s) 137, 219, 440, 918, 1139
TasI AATT 4 cut(s) 98, 354, 475, 1130
Tru1I TTAA 5 cut(s) 57, 339, 371, 416, 801
Tru9I TTAA 5 cut(s) 57, 339, 371, 416, 801
TscAI CASTG 2 cut(s) 498, 794
TspDTI ATGAA 5 cut(s) 293, 352, 615, 679, 720
TspGWI ACGGA 2 cut(s) 324, 753
TspRI CASTG 2 cut(s) 498, 794
VpaK11BI GGWCC 2 cut(s) 380, 945
XceI RCATGY 3 cut(s) 182, 314, 1050
XmiI GTMKAC 1 cut(s) 960
XspI CTAG 2 cut(s) 572, 969
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.