MD17G1178800.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008248
Physical Location & Seq
Reverse (-)
20494945 .. 20497174
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1178800.v1.1.491

Sequence Viewer

Length: 612 bp
ATGCAATCAAGGGGGGTGGCATTGGAGGAAGGCAAGGATCCTACAACTACATTTAAGTCCAGCCATGCCAGGGCCCTGCCATTGCGACTCGTTCAATTTCTTGTCATGTTTGTGCTTTTGGGTATTGGGGTTTCGATTTTGAGTATGCACACAATTAGGTTTTTTGGTGTTCAACATGTGGCTCCAACAGCACCTTCCACCATTAGGCCTTGCTTCGAAGAGCCGAGTAGTTTAGAAAGTCGGATTAGACCTCCATCTAACCTGTTGCACGCCATGAATGACACCGAGTTGCTGTGGCTCGCTTCACATACTACTCAAATAAAGGAGTATCCGTTTAAGAGAGTTCCAAAAATTGCCTTCATGTTCTTGACAAAGGGACCATTGCCAATGGAGCCTCTTTGGGAGCGGTTTTTTAGAGGACACGAGGGGATGTATTCGATCTATGTTCATTCGTTGCCATCTTATAATGCCAATTTCTCATCTACATCAGTGTTTTACAAGAGACAAATCCCAAGCCAGGTATTGAAAGATGAACTCCTCCAACCCATGTTGGATTGGACCATCCCATTGTACTGTTGCCGAGTGGGGAGAGATGAGTATGTGCGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

204

Amino Acids

23.35

Weight (kDa)

9.12

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 118 - 174 3.8e-15 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AccBSI CCGCTC 1 cut(s) 406
AciI CCGC 1 cut(s) 406
AclWI GGATC 2 cut(s) 32, 45
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 3 cut(s) 70, 204, 517
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 3 cut(s) 95, 173, 526
AjnI CCWGG 2 cut(s) 68, 516
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
Alw26I GTCTC 1 cut(s) 496
AlwI GGATC 2 cut(s) 32, 45
AoxI GGCC 2 cut(s) 72, 206
ApaI GGGCCC 1 cut(s) 76
AspS9I GGNCC 4 cut(s) 72, 73, 377, 558
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 2 cut(s) 377, 558
BaeGI GKGCMC 1 cut(s) 76
BamHI GGATCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 76
BauI CACGAG 1 cut(s) 422
BccI CCATC 3 cut(s) 262, 466, 569
BciT130I CCWGG 2 cut(s) 70, 518
BciVI GTATCC 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 339
Bme1390I CCNGG 2 cut(s) 70, 518
Bme18I GGWCC 2 cut(s) 377, 558
BmgT120I GGNCC 4 cut(s) 72, 73, 377, 558
BmiI GGNNCC 5 cut(s) 39, 74, 183, 378, 393
BmrFI CCNGG 2 cut(s) 70, 518
BmsI GCATC 1 cut(s) 596
Bpu14I TTCGAA 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 204, 517
Bse3DI GCAATG 2 cut(s) 80, 380
BseBI CCWGG 2 cut(s) 70, 518
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 2 cut(s) 435, 561
BseLI CCNNNNNNNGG 3 cut(s) 70, 204, 517
BseMI GCAATG 2 cut(s) 80, 380
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 76
BshFI GGCC 2 cut(s) 74, 208
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 3 cut(s) 70, 204, 517
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 2 cut(s) 74, 208
Bsp119I TTCGAA 1 cut(s) 216
Bsp120I GGGCCC 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 37, 438
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 74, 208
BspLI GGNNCC 5 cut(s) 39, 74, 183, 378, 393
BspPI GGATC 2 cut(s) 32, 45
BspQI GCTCTTC 1 cut(s) 213
BspT104I TTCGAA 1 cut(s) 216
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 2 cut(s) 80, 380
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 37, 438
BssSI CACGAG 1 cut(s) 422
Bst2BI CACGAG 1 cut(s) 422
Bst2UI CCWGG 2 cut(s) 70, 518
Bst4CI ACNGT 1 cut(s) 575
Bst6I CTCTTC 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 270, 300
BstF5I GGATG 2 cut(s) 435, 561
BstKTI GATC 2 cut(s) 40, 441
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 2 cut(s) 37, 438
BstMWI GCNNNNNNNGC 2 cut(s) 188, 391
BstNI CCWGG 2 cut(s) 70, 518
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 2 cut(s) 68, 516
BstSLI GKGCMC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 339
BsuRI GGCC 2 cut(s) 74, 208
BtsCI GGATG 2 cut(s) 435, 561
BtsIMutI CAGTG 1 cut(s) 495
Cac8I GCNNGC 2 cut(s) 270, 300
Cfr13I GGNCC 4 cut(s) 72, 73, 377, 558
Csp6I GTAC 1 cut(s) 571
CspCI CAANNNNNGTGG 1 cut(s) 32
CviAII CATG 6 cut(s) 65, 106, 176, 274, 361, 547
CviJI RGCY 8 cut(s) 63, 74, 182, 208, 223, 298, 394, 516
CviKI_1 RGCY 8 cut(s) 63, 74, 182, 208, 223, 298, 394, 516
CviQI GTAC 1 cut(s) 571
DpnI GATC 2 cut(s) 39, 440
DpnII GATC 2 cut(s) 37, 438
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 377, 558
EcoO109I RGGNCCY 2 cut(s) 72, 73
EcoRII CCWGG 2 cut(s) 68, 516
EcoT38I GRGCYC 1 cut(s) 76
FaeI CATG 6 cut(s) 68, 109, 179, 277, 364, 550
FaqI GGGAC 1 cut(s) 390
FatI CATG 6 cut(s) 64, 105, 175, 273, 360, 546
FokI GGATG 2 cut(s) 442, 548
FriOI GRGCYC 1 cut(s) 76
HaeIII GGCC 2 cut(s) 74, 208
Hin1II CATG 6 cut(s) 68, 109, 179, 277, 364, 550
HinfI GANTC 1 cut(s) 87
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 1 cut(s) 367
HpyAV CCTTC 3 cut(s) 23, 204, 367
HpyCH4III ACNGT 1 cut(s) 575
HpyCH4V TGCA 3 cut(s) 4, 148, 268
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 391
Hsp92II CATG 6 cut(s) 68, 109, 179, 277, 364, 550
Kzo9I GATC 2 cut(s) 37, 438
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 3 cut(s) 187, 391, 403
LpnPI CCDG 7 cut(s) 55, 73, 82, 89, 275, 503, 530
LweI GCATC 1 cut(s) 596
MalI GATC 2 cut(s) 39, 440
MbiI CCGCTC 1 cut(s) 406
MboI GATC 2 cut(s) 37, 438
MboII GAAGA 1 cut(s) 230
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 4 cut(s) 95, 153, 351, 472
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 4 cut(s) 209, 221, 531, 565
MnlI CCTC 6 cut(s) 19, 261, 405, 410, 418, 548
MseI TTAA 2 cut(s) 54, 336
MslI CAYNNNNRTG 1 cut(s) 110
MspR9I CCNGG 2 cut(s) 70, 518
MvaI CCWGG 2 cut(s) 70, 518
MwoI GCNNNNNNNGC 2 cut(s) 188, 391
NdeII GATC 2 cut(s) 37, 438
NlaIII CATG 6 cut(s) 68, 109, 179, 277, 364, 550
NlaIV GGNNCC 5 cut(s) 39, 74, 183, 378, 393
NmeAIII GCCGAG 2 cut(s) 249, 605
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 1 cut(s) 216
PceI AGGCCT 1 cut(s) 208
PciI ACATGT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 213
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 175
PsiI TTATAA 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 68, 516
PspGI CCWGG 2 cut(s) 68, 516
PspN4I GGNNCC 5 cut(s) 39, 74, 183, 378, 393
PspOMI GGGCCC 1 cut(s) 72
PspPI GGNCC 4 cut(s) 72, 73, 377, 558
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 2 cut(s) 54, 336
Sau3AI GATC 2 cut(s) 37, 438
Sau96I GGNCC 4 cut(s) 72, 73, 377, 558
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 70, 518
SduI GDGCHC 1 cut(s) 76
SetI ASST 5 cut(s) 161, 196, 253, 264, 522
SfaNI GCATC 1 cut(s) 596
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 2 cut(s) 377, 558
SmiMI CAYNNNNRTG 1 cut(s) 110
Sse9I AATT 4 cut(s) 95, 153, 351, 472
SseBI AGGCCT 1 cut(s) 208
SsiI CCGC 1 cut(s) 406
StuI AGGCCT 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 68, 516
TaaI ACNGT 1 cut(s) 575
TaqI TCGA 3 cut(s) 134, 216, 437
TasI AATT 4 cut(s) 95, 153, 351, 472
TatI WGTACW 1 cut(s) 570
Tru1I TTAA 2 cut(s) 54, 336
Tru9I TTAA 2 cut(s) 54, 336
TscAI CASTG 1 cut(s) 495
TspDTI ATGAA 4 cut(s) 290, 349, 437, 546
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 1 cut(s) 495
VpaK11BI GGWCC 2 cut(s) 377, 558
XceI RCATGY 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.