AT3G23810

adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009985
Physical Location & Seq
Reverse (-)
8587622 .. 8589754
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23810.1

Sequence Viewer

Length: 1458 bp
ATGGCTTTGCTTGTAGAGAAAACCTCAAGTGGCCGTGAATACAAGGTCAAGGACATGTCCCAAGCCGACTTCGGCCGTCTCGAAATCGAGCTAGCCGAAGTCGAAATGCCTGGACTTGTCTCTTGCGTTACCGAATTCGGACCATCTCAGCCACTCAAAGGAGCAAGAATCACCGGTTCTCTCCACATGACGATCCAAACCGCGGTTTTAATCGAAACCTTAACCGCTCTCGGCGCTGAAGTCAGATGGTGTTCTTGCAACATCTTCTCCACTCAAGACCACGCCGCGGCCGCTATTGCTCGTGATTCCGCCGCGGTGTTCGCTTGGAAAGGTGAGACTCTTCAAGAATATTGGTGGTGTACAGAGAGAGCTCTTGACTGGGGTCCTGGAGGTGGTCCTGATCTCATCGTTGATGACGGTGGTGATGCTACACTGTTGATCCATGAAGGAGTTAAAGCTGAAGAGATCTTTGCCAAGAATGGTACGTTCCCTGACCCGACCTCTACTGATAATCCCGAGTTTCAGATCGTCCTGTCTATTATCAAAGACGGTCTTCAAGTTGATCCCAAGAAGTACCATAAGATGAAGGAGAGATTGGTTGGTGTCTCTGAGGAGACTACTACTGGTGTTAAGAGGCTTTATCAGATGCAGGAGACTGGAGCTCTCTTGTTCCCTGCCATTAACGTCAATGACTCTGTCACCAAGAGCAAGTTTGACAACTTGTACGGATGCCGACACTCGCTTCCCGATGGTCTCATGAGGGCTACTGATGTTATGATTGCTGGAAAGGTTGCTGTTATCTGTGGTTATGGTGATGTAGGAAAAGGTTGTGCTGCAGCAATGAAAACCGCGGGTGCTCGTGTCATAGTGACTGAGATTGATCCAATCTGTGCTCTTCAGGCTCTTATGGAAGGTCTTCAAGTTCTGACCCTTGAGGATGTTGTCTCAGAAGCTGATATCTTCTGTACCACTACTGGTAACAAAGATATTATTATGGTTGATCACATGAGGAAGATGAAGAACAATGCCATTGTCTGTAACATTGGTCATTTTGACAATGAAATCGACATGCTTGGACTTGAGACCTACCCTGGTGTGAAACGTATTACCATCAAGCCACAGACCGATAGGTGGGTGTTTCCTGATACCAACTCTGGCATCATTGTTCTAGCTGAAGGTCGTTTGATGAACTTGGGATGCGCCACTGGACACCCGAGTTTCGTGATGTCGTGCTCATTCACCAACCAGGTGATTGCACAGCTTGAGCTCTGGAACGAGAAGTCAAGCGGGAAGTATGAGAAGAAGGTCTATGTTTTGCCTAAACACTTGGATGAGAAGGTAGCTGCACTTCACTTAGGCAAGCTTGGTGCAAGACTCACCAAGCTTACCAAGGACCAATCTGACTATGTCAGCATACCCGTTGAAGGTCCTTACAAGCCTGTTCACTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000096 GO:0003006 GO:0003674 GO:0003824 GO:0004013 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016787 GO:0016801 GO:0016802 GO:0017144 GO:0019219 GO:0019222 GO:0019752 GO:0022414 GO:0030054 GO:0031090 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0033353 GO:0034641 GO:0040029 GO:0042278 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0046872 GO:0046914 GO:0048046 GO:0048316 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0055086 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0072521 GO:0080090 GO:0090304 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564 GO:1901605 GO:1901657 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

485

Amino Acids

53.16

Weight (kDa)

5.49

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 13 - 484 1.3e-143 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 240 - 403 4.3e-83 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 259 - 349 2.5e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 227
AccII CGCG 4 cut(s) 203, 287, 314, 851
AclWI GGATC 4 cut(s) 187, 433, 557, 875
AcoI YGGCCR 3 cut(s) 31, 73, 288
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 4 cut(s) 258, 480, 881, 1194
AfaI GTAC 6 cut(s) 361, 484, 575, 725, 967, 1454
AfiI CCNNNNNNNGG 6 cut(s) 158, 202, 286, 392, 1131, 1424
AflIII ACRYGT 1 cut(s) 54
AgeI ACCGGT 1 cut(s) 173
AgsI TTSAA 4 cut(s) 344, 557, 920, 1424
AjnI CCWGG 4 cut(s) 109, 385, 1090, 1245
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 6 cut(s) 373, 664, 859, 895, 1235, 1269
Alw26I GTCTC 9 cut(s) 83, 124, 329, 608, 610, 647, 758, 949, 1076
AlwI GGATC 4 cut(s) 187, 433, 557, 875
AlwNI CAGNNNCTG 2 cut(s) 953, 1455
Ama87I CYCGRG 2 cut(s) 515, 1213
AoxI GGCC 3 cut(s) 31, 73, 288
ApeKI GCWGC 3 cut(s) 833, 836, 1343
ApoI RAATTY 1 cut(s) 134
AsiGI ACCGGT 1 cut(s) 173
Asp700I GAANNNNTTC 1 cut(s) 915
AspLEI GCGC 2 cut(s) 236, 1202
AspS9I GGNCC 5 cut(s) 140, 383, 395, 1393, 1427
AsuHPI GGTGA 8 cut(s) 163, 344, 434, 691, 824, 1231, 1261, 1369
AsuNHI GCTAGC 1 cut(s) 91
AvaI CYCGRG 2 cut(s) 515, 1213
AvaII GGWCC 5 cut(s) 140, 383, 395, 1393, 1427
BanII GRGCYC 3 cut(s) 373, 664, 1269
BarI GAAGNNNNNNTAC 2 cut(s) 1332, 1364
BauI CACGAG 2 cut(s) 300, 858
BbsI GAAGAC 2 cut(s) 545, 908
Bbv12I GWGCWC 6 cut(s) 373, 664, 859, 895, 1235, 1269
BbvI GCAGC 3 cut(s) 820, 848, 1330
BccI CCATC 4 cut(s) 151, 240, 743, 1118
BceAI ACGGC 2 cut(s) 18, 60
BciT130I CCWGG 4 cut(s) 111, 387, 1092, 1247
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 9 cut(s) 83, 124, 329, 608, 610, 647, 758, 949, 1076
BfaI CTAG 2 cut(s) 92, 1169
BfmI CTRYAG 2 cut(s) 834, 1447
BfoI RGCGCY 1 cut(s) 237
BglII AGATCT 1 cut(s) 465
BisI GCNGC 7 cut(s) 285, 288, 291, 312, 834, 837, 1344
BlsI GCNGC 7 cut(s) 286, 289, 292, 313, 835, 838, 1345
Bme1390I CCNGG 4 cut(s) 111, 387, 1092, 1247
Bme18I GGWCC 5 cut(s) 140, 383, 395, 1393, 1427
BmeT110I CYCGRG 2 cut(s) 515, 1213
BmgT120I GGNCC 5 cut(s) 140, 383, 395, 1393, 1427
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 4 cut(s) 111, 387, 1092, 1247
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 5 cut(s) 415, 636, 719, 1167, 1187
BmtI GCTAGC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 388
BoxI GACNNNNGTC 1 cut(s) 381
BpiI GAAGAC 2 cut(s) 545, 908
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 2 cut(s) 408, 678
BpuEI CTTGAG 5 cut(s) 10, 258, 953, 1100, 1283
BsaI GGTCTC 2 cut(s) 758, 1076
BsaJI CCNNGG 6 cut(s) 201, 285, 312, 849, 1090, 1389
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 202, 286, 392, 1131, 1424
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 5 cut(s) 383, 628, 661, 979, 1210
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 4 cut(s) 111, 387, 1092, 1247
BseDI CCNNGG 6 cut(s) 201, 285, 312, 849, 1090, 1389
BseGI GGATG 4 cut(s) 734, 943, 1202, 1336
BseLI CCNNNNNNNGG 6 cut(s) 158, 202, 286, 392, 1131, 1424
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 4 cut(s) 161, 600, 864, 960
BseNI ACTGG 5 cut(s) 383, 628, 661, 979, 1210
BseRI GAGGAG 1 cut(s) 626
BseX3I CGGCCG 2 cut(s) 73, 288
BseXI GCAGC 3 cut(s) 820, 848, 1330
BsgI GTGCAG 1 cut(s) 1329
Bsh1236I CGCG 4 cut(s) 203, 287, 314, 851
Bsh1285I CGRYCG 2 cut(s) 76, 291
BshFI GGCC 3 cut(s) 33, 75, 290
BshTI ACCGGT 1 cut(s) 173
BsiEI CGRYCG 2 cut(s) 76, 291
BsiHKAI GWGCWC 6 cut(s) 373, 664, 859, 895, 1235, 1269
BsiHKCI CYCGRG 2 cut(s) 515, 1213
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 6 cut(s) 158, 202, 286, 392, 1131, 1424
BsmAI GTCTC 9 cut(s) 83, 124, 329, 608, 610, 647, 758, 949, 1076
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 3 cut(s) 33, 75, 290
Bso31I GGTCTC 2 cut(s) 758, 1076
BsoBI CYCGRG 2 cut(s) 515, 1213
Bsp1286I GDGCHC 6 cut(s) 373, 664, 859, 895, 1235, 1269
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
BspANI GGCC 3 cut(s) 33, 75, 290
BspCNI CTCAG 4 cut(s) 160, 601, 865, 959
BspFNI CGCG 4 cut(s) 203, 287, 314, 851
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 384
BspMAI CTGCAG 1 cut(s) 838
BspOI GCTAGC 1 cut(s) 95
BspPI GGATC 4 cut(s) 187, 433, 557, 875
BspQI GCTCTTC 1 cut(s) 900
BspTNI GGTCTC 2 cut(s) 758, 1076
BsrBI CCGCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 359
BsrI ACTGG 5 cut(s) 383, 628, 661, 979, 1210
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 6 cut(s) 201, 285, 312, 849, 1090, 1389
BssMI GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
BssSI CACGAG 2 cut(s) 300, 858
BssT1I CCWWGG 1 cut(s) 1389
Bst2BI CACGAG 2 cut(s) 300, 858
Bst2UI CCWGG 4 cut(s) 111, 387, 1092, 1247
Bst4CI ACNGT 3 cut(s) 419, 435, 551
Bst6I CTCTTC 3 cut(s) 345, 456, 900
BstAUI TGTACA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 93, 1361
BstDEI CTNAG 5 cut(s) 147, 609, 873, 946, 1354
BstDSI CCRYGG 4 cut(s) 201, 285, 312, 849
BstF5I GGATG 4 cut(s) 734, 943, 1202, 1336
BstFNI CGCG 4 cut(s) 203, 287, 314, 851
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 1202
BstKTI GATC 8 cut(s) 195, 403, 441, 468, 528, 565, 883, 1003
BstMAI GTCTC 9 cut(s) 83, 124, 329, 608, 610, 647, 758, 949, 1076
BstMBI GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
BstMCI CGRYCG 2 cut(s) 76, 291
BstMWI GCNNNNNNNGC 5 cut(s) 233, 290, 296, 320, 899
BstNI CCWGG 4 cut(s) 111, 387, 1092, 1247
BstNSI RCATGY 2 cut(s) 58, 1072
BstPAI GACNNNNGTC 1 cut(s) 381
BstSCI CCNGG 4 cut(s) 109, 385, 1090, 1245
BstSFI CTRYAG 2 cut(s) 834, 1447
BstUI CGCG 4 cut(s) 203, 287, 314, 851
BstV1I GCAGC 3 cut(s) 820, 848, 1330
BstV2I GAAGAC 2 cut(s) 545, 908
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BstZI CGGCCG 2 cut(s) 73, 288
BsuRI GGCC 3 cut(s) 33, 75, 290
BtgI CCRYGG 4 cut(s) 201, 285, 312, 849
BtsCI GGATG 4 cut(s) 734, 943, 1202, 1336
BtsIMutI CAGTG 2 cut(s) 431, 1203
Cac8I GCNNGC 2 cut(s) 93, 1361
CaiI CAGNNNCTG 2 cut(s) 953, 1455
CciI TCATGA 1 cut(s) 756
CciNI GCGGCCGC 1 cut(s) 288
CfoI GCGC 2 cut(s) 236, 1202
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 5 cut(s) 140, 383, 395, 1393, 1427
Cfr42I CCGCGG 4 cut(s) 204, 288, 315, 852
CsiI ACCWGGT 1 cut(s) 1245
Csp6I GTAC 6 cut(s) 360, 483, 574, 724, 966, 1453
CspAI ACCGGT 1 cut(s) 173
CviAII CATG 6 cut(s) 55, 187, 443, 757, 1006, 1069
CviQI GTAC 6 cut(s) 360, 483, 574, 724, 966, 1453
DdeI CTNAG 5 cut(s) 147, 609, 873, 946, 1354
DpnI GATC 8 cut(s) 194, 402, 440, 467, 527, 564, 882, 1002
DpnII GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
EaeI YGGCCR 3 cut(s) 31, 73, 288
EagI CGGCCG 2 cut(s) 73, 288
Eam1104I CTCTTC 3 cut(s) 345, 456, 900
EarI CTCTTC 3 cut(s) 345, 456, 900
EciI GGCGGA 1 cut(s) 298
Ecl136II GAGCTC 3 cut(s) 371, 662, 1267
EclXI CGGCCG 2 cut(s) 73, 288
Eco130I CCWWGG 1 cut(s) 1389
Eco24I GRGCYC 3 cut(s) 373, 664, 1269
Eco31I GGTCTC 2 cut(s) 758, 1076
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 5 cut(s) 140, 383, 395, 1393, 1427
Eco52I CGGCCG 2 cut(s) 73, 288
Eco53kI GAGCTC 3 cut(s) 371, 662, 1267
Eco57I CTGAAG 4 cut(s) 258, 480, 881, 1194
Eco88I CYCGRG 2 cut(s) 515, 1213
EcoICRI GAGCTC 3 cut(s) 371, 662, 1267
EcoO109I RGGNCCY 2 cut(s) 383, 1427
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 4 cut(s) 109, 385, 1090, 1245
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 1389
EcoT38I GRGCYC 3 cut(s) 373, 664, 1269
ErhI CCWWGG 1 cut(s) 1389
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 6 cut(s) 58, 190, 446, 760, 1009, 1072
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 43
FatI CATG 6 cut(s) 54, 186, 442, 756, 1005, 1068
FauI CCCGC 2 cut(s) 844, 1280
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 7 cut(s) 285, 288, 291, 312, 834, 837, 1344
FokI GGATG 4 cut(s) 741, 950, 1209, 1343
FriOI GRGCYC 3 cut(s) 373, 664, 1269
Fsp4HI GCNGC 7 cut(s) 285, 288, 291, 312, 834, 837, 1344
FspBI CTAG 2 cut(s) 92, 1169
GlaI GCGC 2 cut(s) 235, 1201
GluI GCNGC 7 cut(s) 285, 288, 291, 312, 834, 837, 1344
GsuI CTGGAG 2 cut(s) 408, 678
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 3 cut(s) 33, 75, 290
HapII CCGG 1 cut(s) 174
HhaI GCGC 2 cut(s) 236, 1202
Hin1II CATG 6 cut(s) 58, 190, 446, 760, 1009, 1072
Hin6I GCGC 2 cut(s) 234, 1200
HinP1I GCGC 2 cut(s) 234, 1200
HindIII AAGCTT 2 cut(s) 1361, 1382
HinfI GANTC 5 cut(s) 168, 305, 337, 692, 1374
HpaII CCGG 1 cut(s) 174
HphI GGTGA 8 cut(s) 163, 344, 434, 691, 824, 1231, 1261, 1369
Hpy166II GTNNAC 2 cut(s) 360, 1444
Hpy188I TCNGA 8 cut(s) 140, 245, 525, 610, 645, 927, 949, 1402
Hpy8I GTNNAC 2 cut(s) 360, 1444
HpyAV CCTTC 7 cut(s) 440, 580, 905, 1169, 1297, 1330, 1418
HpyCH4III ACNGT 3 cut(s) 419, 435, 551
HpyCH4IV ACGT 3 cut(s) 485, 684, 1102
HpyCH4V TGCA 6 cut(s) 258, 649, 836, 1256, 1346, 1370
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 290, 296, 320, 899
HpyF3I CTNAG 5 cut(s) 147, 609, 873, 946, 1354
HpySE526I ACGT 3 cut(s) 485, 684, 1102
Hsp92II CATG 6 cut(s) 58, 190, 446, 760, 1009, 1072
HspAI GCGC 2 cut(s) 234, 1200
Ksp22I TGATCA 1 cut(s) 1000
KspI CCGCGG 4 cut(s) 204, 288, 315, 852
Kzo9I GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
LguI GCTCTTC 1 cut(s) 900
LmnI GCTCC 2 cut(s) 161, 659
Lsp1109I GCAGC 3 cut(s) 820, 848, 1330
LweI GCATC 5 cut(s) 415, 636, 719, 1167, 1187
MabI ACCWGGT 1 cut(s) 1245
MaeI CTAG 2 cut(s) 92, 1169
MaeII ACGT 3 cut(s) 485, 684, 1102
MaeIII GTNAC 5 cut(s) 127, 697, 868, 977, 1037
MalI GATC 8 cut(s) 194, 402, 440, 467, 527, 564, 882, 1002
MbiI CCGCTC 1 cut(s) 227
MboI GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 6 cut(s) 373, 664, 859, 895, 1235, 1269
MluCI AATT 1 cut(s) 134
MlyI GAGTC 3 cut(s) 331, 686, 1368
MnlI CCTC 8 cut(s) 34, 383, 511, 604, 627, 753, 928, 1002
MroXI GAANNNNTTC 1 cut(s) 915
MseI TTAA 5 cut(s) 209, 221, 453, 630, 681
MslI CAYNNNNRTG 1 cut(s) 1329
MspA1I CMGCKG 4 cut(s) 203, 287, 314, 851
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 4 cut(s) 111, 387, 1092, 1247
MvaI CCWGG 4 cut(s) 111, 387, 1092, 1247
MvnI CGCG 4 cut(s) 203, 287, 314, 851
MwoI GCNNNNNNNGC 5 cut(s) 233, 290, 296, 320, 899
NdeII GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 6 cut(s) 58, 190, 446, 760, 1009, 1072
NlaIV GGNNCC 1 cut(s) 384
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 2 cut(s) 697, 868
NotI GCGGCCGC 1 cut(s) 288
NspI RCATGY 2 cut(s) 58, 1072
PagI TCATGA 1 cut(s) 756
PciI ACATGT 1 cut(s) 54
PciSI GCTCTTC 1 cut(s) 900
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 93
PdmI GAANNNNTTC 1 cut(s) 915
PfeI GAWTC 2 cut(s) 168, 305
PflFI GACNNNGTC 2 cut(s) 695, 1406
PfoI TCCNGGA 1 cut(s) 385
PinAI ACCGGT 1 cut(s) 173
PkrI GCNGC 7 cut(s) 286, 289, 292, 313, 835, 838, 1345
PleI GAGTC 3 cut(s) 331, 686, 1368
PpsI GAGTC 3 cut(s) 331, 686, 1368
PpuMI RGGWCCY 2 cut(s) 383, 1427
PscI ACATGT 1 cut(s) 54
PshAI GACNNNNGTC 1 cut(s) 381
Psp124BI GAGCTC 3 cut(s) 373, 664, 1269
Psp5II RGGWCCY 2 cut(s) 383, 1427
Psp6I CCWGG 4 cut(s) 109, 385, 1090, 1245
PspGI CCWGG 4 cut(s) 109, 385, 1090, 1245
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 5 cut(s) 140, 383, 395, 1393, 1427
PspPPI RGGWCCY 2 cut(s) 383, 1427
PstI CTGCAG 1 cut(s) 838
PstNI CAGNNNCTG 2 cut(s) 953, 1455
PsuI RGATCY 1 cut(s) 465
PsyI GACNNNGTC 2 cut(s) 695, 1406
RsaI GTAC 6 cut(s) 361, 484, 575, 725, 967, 1454
RsaNI GTAC 6 cut(s) 360, 483, 574, 724, 966, 1453
RseI CAYNNNNRTG 1 cut(s) 1329
SacI GAGCTC 3 cut(s) 373, 664, 1269
SacII CCGCGG 4 cut(s) 204, 288, 315, 852
SapI GCTCTTC 1 cut(s) 900
SaqAI TTAA 5 cut(s) 209, 221, 453, 630, 681
SatI GCNGC 7 cut(s) 285, 288, 291, 312, 834, 837, 1344
Sau3AI GATC 8 cut(s) 192, 400, 438, 465, 525, 562, 880, 1000
Sau96I GGNCC 5 cut(s) 140, 383, 395, 1393, 1427
SchI GAGTC 3 cut(s) 331, 686, 1368
ScrFI CCNGG 4 cut(s) 111, 387, 1092, 1247
SduI GDGCHC 6 cut(s) 373, 664, 859, 895, 1235, 1269
SexAI ACCWGGT 1 cut(s) 1245
SfaNI GCATC 5 cut(s) 415, 636, 719, 1167, 1187
SfcI CTRYAG 2 cut(s) 834, 1447
Sfr303I CCGCGG 4 cut(s) 204, 288, 315, 852
SgrBI CCGCGG 4 cut(s) 204, 288, 315, 852
SinI GGWCC 5 cut(s) 140, 383, 395, 1393, 1427
SmiMI CAYNNNNRTG 1 cut(s) 1329
SmlI CTYRAG 5 cut(s) 25, 273, 932, 1079, 1262
SmoI CTYRAG 5 cut(s) 25, 273, 932, 1079, 1262
Sse9I AATT 1 cut(s) 134
SspI AATATT 1 cut(s) 350
SspMI CTAG 2 cut(s) 92, 1169
SstI GAGCTC 3 cut(s) 373, 664, 1269
StyD4I CCNGG 4 cut(s) 109, 385, 1090, 1245
StyI CCWWGG 1 cut(s) 1389
TaaI ACNGT 3 cut(s) 419, 435, 551
TaiI ACGT 3 cut(s) 488, 687, 1105
TaqI TCGA 5 cut(s) 81, 87, 102, 213, 1065
TaqII GACCGA 1 cut(s) 1139
TasI AATT 1 cut(s) 134
TatI WGTACW 1 cut(s) 359
TauI GCSGC 4 cut(s) 287, 290, 293, 314
TfiI GAWTC 2 cut(s) 168, 305
Tru1I TTAA 5 cut(s) 209, 221, 453, 630, 681
Tru9I TTAA 5 cut(s) 209, 221, 453, 630, 681
TscAI CASTG 2 cut(s) 438, 1210
TseFI GTSAC 2 cut(s) 697, 868
TseI GCWGC 3 cut(s) 833, 836, 1343
Tsp45I GTSAC 2 cut(s) 697, 868
TspDTI ATGAA 6 cut(s) 459, 599, 857, 1031, 1074, 1202
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 2 cut(s) 438, 1210
Tth111I GACNNNGTC 2 cut(s) 695, 1406
VpaK11BI GGWCC 5 cut(s) 140, 383, 395, 1393, 1427
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 58, 1072
XmnI GAANNNNTTC 1 cut(s) 915
XspI CTAG 2 cut(s) 92, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.