MD05G1230000.v1.1

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009985
Physical Location & Seq
Reverse (-)
36357662 .. 36358748
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1230000.v1.1.491

Sequence Viewer

Length: 798 bp
ATGAAGGACAGGTTGGTCGGAGTCTCGGAGGAGACCACCACCGGCGTCAAGAGATTGTATCAGATGCAGGCCAGCGGCGCTCTCTTGTTCCCCGCCATCAATGTCAACGACTCTGTTACCAAGAGCAAGGTTTCCGTTGTCTGTGGATATGGAGATGTCGGAAAGGGTTGTGCTGCTGCCCTCAAGCAAGCTGGATCCCGCGTGGTTGTGACTGAGATTGATCCAATCTGTGCCCTCCAGGCTCTTATGGAAGGCCTTCAGGTTCTTCCCCTTGAGGATGTTGTCGCTGAGGCCGATATCTTTGTTACCACCACCGGTAACAAGGACATCATCATGGTTGACCACATGAAGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGTCATTTTGACAATGAGATTGACATGCATGGTCTTGAGACATACCCCGGAGTGAAGCGCATCACCATCAAGCCTCAAACCGACAGGTGGGTCTTCCCCGAGACCAAATCCGGCATCATTGTGTTGGCTGAGGGCCGTCTCATGAACTTGGGCTGTGCCACTGGACACCCCAGCTTTGTGATGTCCTGCTCTTTCACCAACCAAGTGATTGCTCAGCTCGAGTTGTGGAATGAGAGGAAGTCCGGCAAGTACGAGAAGAAGGTGTATGTCTTGCCCAAGCACCTTGATGAGAAGGTTGCTGCCCTTCATCTTGGAAAGCTTGGAGCTAAGCTCACCAAGCTCACCAAAGAGCAGGCTGACTACATCAGCGTGCCGGTCGAGGGTCCATACAAGCCAGCTGCCTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

266

Amino Acids

29.04

Weight (kDa)

8.41

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 3 - 264 2.9e-59 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 36 - 183 2.3e-69 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 41 - 129 6.8e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 14, 473
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 75, 93, 199
AclWI GGATC 3 cut(s) 189, 202, 215
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 635, 794
AfiI CCNNNNNNNGG 2 cut(s) 471, 764
AgeI ACCGGT 1 cut(s) 314
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 8 cut(s) 191, 558, 601, 703, 710, 715, 724, 782
AluI AGCT 8 cut(s) 191, 558, 601, 703, 710, 715, 724, 782
Alw26I GTCTC 5 cut(s) 26, 28, 416, 479, 527
AlwI GGATC 3 cut(s) 189, 202, 215
AlwNI CAGNNNCTG 1 cut(s) 795
Ama87I CYCGRG 2 cut(s) 482, 602
AoxI GGCC 4 cut(s) 69, 253, 291, 517
ApeKI GCWGC 4 cut(s) 173, 176, 683, 782
AsiGI ACCGGT 1 cut(s) 314
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 2 cut(s) 80, 444
AspS9I GGNCC 2 cut(s) 517, 767
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 4 cut(s) 439, 571, 709, 718
AvaI CYCGRG 2 cut(s) 482, 602
AvaII GGWCC 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 235
BamHI GGATCC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 469
BbvCI CCTCAGC 2 cut(s) 288, 513
BbvI GCAGC 4 cut(s) 160, 163, 670, 769
BccI CCATC 2 cut(s) 104, 458
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 5 cut(s) 26, 28, 416, 479, 527
BfmI CTRYAG 1 cut(s) 787
BfoI RGCGCY 1 cut(s) 81
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 5 cut(s) 76, 174, 177, 684, 783
BlpI GCTNAGC 2 cut(s) 597, 711
BlsI GCNGC 5 cut(s) 77, 175, 178, 685, 784
Bme1390I CCNGG 2 cut(s) 239, 432
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 2 cut(s) 482, 602
BmgT120I GGNCC 2 cut(s) 517, 767
BmiI GGNNCC 2 cut(s) 196, 768
BmrFI CCNGG 2 cut(s) 239, 432
BmsI GCATC 3 cut(s) 54, 453, 507
BpiI GAAGAC 1 cut(s) 469
BplI GAGNNNNNCTC 2 cut(s) 699, 731
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 288, 513
Bpu1102I GCTNAGC 2 cut(s) 597, 711
BpuEI CTTGAG 3 cut(s) 167, 293, 440
BpuMI CCSGG 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 2 cut(s) 26, 479
BsaJI CCNNGG 1 cut(s) 430
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 764
Bse118I RCCGGY 3 cut(s) 41, 314, 757
Bse1I ACTGG 1 cut(s) 550
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 471, 764
BseMII CTCAG 4 cut(s) 204, 279, 504, 611
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 4 cut(s) 160, 163, 670, 769
BseYI CCCAGC 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 762
BshFI GGCC 4 cut(s) 71, 255, 293, 519
BshTI ACCGGT 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 762
BsiHKCI CYCGRG 2 cut(s) 482, 602
BsiSI CCGG 6 cut(s) 42, 315, 432, 495, 627, 758
BslI CCNNNNNNNGG 2 cut(s) 471, 764
BsmAI GTCTC 5 cut(s) 26, 28, 416, 479, 527
BsmBI CGTCTC 1 cut(s) 527
BsnI GGCC 4 cut(s) 71, 255, 293, 519
Bso31I GGTCTC 2 cut(s) 26, 479
BsoBI CYCGRG 2 cut(s) 482, 602
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 194, 220
Bsp1720I GCTNAGC 2 cut(s) 597, 711
BspACI CCGC 3 cut(s) 75, 93, 199
BspANI GGCC 4 cut(s) 71, 255, 293, 519
BspCNI CTCAG 4 cut(s) 205, 280, 505, 610
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 196, 768
BspPI GGATC 3 cut(s) 189, 202, 215
BspTNI GGTCTC 2 cut(s) 26, 479
BsrFI RCCGGY 3 cut(s) 41, 314, 757
BsrI ACTGG 1 cut(s) 550
BssAI RCCGGY 3 cut(s) 41, 314, 757
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 2 cut(s) 194, 220
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 239
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 6 cut(s) 69, 73, 189, 738, 755, 780
BstDEI CTNAG 5 cut(s) 213, 288, 513, 597, 711
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 2 cut(s) 80, 444
BstKTI GATC 2 cut(s) 197, 223
BstMAI GTCTC 5 cut(s) 26, 28, 416, 479, 527
BstMBI GATC 2 cut(s) 194, 220
BstMCI CGRYCG 1 cut(s) 762
BstMWI GCNNNNNNNGC 3 cut(s) 77, 239, 721
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 237, 430
BstSFI CTRYAG 1 cut(s) 787
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 4 cut(s) 160, 163, 670, 769
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuRI GGCC 4 cut(s) 71, 255, 293, 519
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 6 cut(s) 69, 73, 189, 738, 755, 780
CaiI CAGNNNCTG 1 cut(s) 795
CciI TCATGA 1 cut(s) 525
CfoI GCGC 2 cut(s) 80, 444
Cfr10I RCCGGY 3 cut(s) 41, 314, 757
Cfr13I GGNCC 2 cut(s) 517, 767
CseI GACGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 634, 793
CspAI ACCGGT 1 cut(s) 314
CviAII CATG 5 cut(s) 334, 346, 409, 413, 526
CviQI GTAC 2 cut(s) 634, 793
DdeI CTNAG 5 cut(s) 213, 288, 513, 597, 711
DpnI GATC 2 cut(s) 196, 222
DpnII GATC 2 cut(s) 194, 220
DrdI GACNNNNNNGTC 2 cut(s) 14, 473
DseDI GACNNNNNNGTC 2 cut(s) 14, 473
Eco147I AGGCCT 1 cut(s) 255
Eco31I GGTCTC 2 cut(s) 26, 479
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 767
Eco57I CTGAAG 1 cut(s) 242
Eco88I CYCGRG 2 cut(s) 482, 602
EcoRII CCWGG 1 cut(s) 237
EcoRV GATATC 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 5 cut(s) 337, 349, 412, 416, 529
FatI CATG 5 cut(s) 333, 345, 408, 412, 525
FauI CCCGC 2 cut(s) 100, 206
Fnu4HI GCNGC 5 cut(s) 76, 174, 177, 684, 783
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 5 cut(s) 76, 174, 177, 684, 783
GlaI GCGC 2 cut(s) 79, 443
GluI GCNGC 5 cut(s) 76, 174, 177, 684, 783
GsaI CCCAGC 1 cut(s) 558
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 4 cut(s) 71, 255, 293, 519
HapII CCGG 6 cut(s) 42, 315, 432, 495, 627, 758
HgaI GACGC 1 cut(s) 34
HhaI GCGC 2 cut(s) 80, 444
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 5 cut(s) 337, 349, 412, 416, 529
Hin6I GCGC 2 cut(s) 78, 442
HinP1I GCGC 2 cut(s) 78, 442
HincII GTYRAC 2 cut(s) 106, 340
HindII GTYRAC 2 cut(s) 106, 340
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 2 cut(s) 21, 110
HpaII CCGG 6 cut(s) 42, 315, 432, 495, 627, 758
HphI GGTGA 4 cut(s) 439, 571, 709, 718
Hpy166II GTNNAC 2 cut(s) 106, 340
Hpy188I TCNGA 4 cut(s) 20, 28, 63, 161
Hpy188III TCNNGA 3 cut(s) 49, 419, 526
Hpy8I GTNNAC 2 cut(s) 106, 340
HpyAV CCTTC 5 cut(s) 245, 266, 637, 670, 698
HpyCH4V TGCA 3 cut(s) 67, 378, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 239, 721
HpyF3I CTNAG 5 cut(s) 213, 288, 513, 597, 711
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 5 cut(s) 337, 349, 412, 416, 529
HspAI GCGC 2 cut(s) 78, 442
Kzo9I GATC 2 cut(s) 194, 220
LmnI GCTCC 1 cut(s) 707
Lsp1109I GCAGC 4 cut(s) 160, 163, 670, 769
LweI GCATC 3 cut(s) 54, 453, 507
MaeIII GTNAC 4 cut(s) 115, 208, 304, 317
MalI GATC 2 cut(s) 196, 222
MboI GATC 2 cut(s) 194, 220
MboII GAAGA 6 cut(s) 257, 361, 364, 370, 469, 652
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 235
MlyI GAGTC 2 cut(s) 30, 104
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 9 cut(s) 22, 191, 245, 268, 283, 468, 508, 612, 757
Mph1103I ATGCAT 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 255
MslI CAYNNNNRTG 4 cut(s) 332, 503, 669, 752
MspA1I CMGCKG 2 cut(s) 75, 782
MspI CCGG 6 cut(s) 42, 315, 432, 495, 627, 758
MspR9I CCNGG 2 cut(s) 239, 432
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 3 cut(s) 77, 239, 721
NciI CCSGG 1 cut(s) 432
NdeII GATC 2 cut(s) 194, 220
NlaIII CATG 5 cut(s) 337, 349, 412, 416, 529
NlaIV GGNNCC 2 cut(s) 196, 768
NmuCI GTSAC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 414
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 602
PagI TCATGA 1 cut(s) 525
PceI AGGCCT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 255
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 5 cut(s) 77, 175, 178, 685, 784
PleI GAGTC 2 cut(s) 29, 104
PpsI GAGTC 2 cut(s) 29, 104
Psp6I CCWGG 1 cut(s) 237
PspFI CCCAGC 1 cut(s) 554
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 196, 768
PspPI GGNCC 2 cut(s) 517, 767
PspXI VCTCGAGB 1 cut(s) 602
PstNI CAGNNNCTG 1 cut(s) 795
PsuI RGATCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 2 cut(s) 635, 794
RsaNI GTAC 2 cut(s) 634, 793
RseI CAYNNNNRTG 4 cut(s) 332, 503, 669, 752
SatI GCNGC 5 cut(s) 76, 174, 177, 684, 783
Sau3AI GATC 2 cut(s) 194, 220
Sau96I GGNCC 2 cut(s) 517, 767
SchI GAGTC 2 cut(s) 30, 104
ScrFI CCNGG 2 cut(s) 239, 432
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 3 cut(s) 54, 453, 507
SfcI CTRYAG 1 cut(s) 787
Sfr274I CTCGAG 1 cut(s) 602
SgrAI CRCCGGYG 1 cut(s) 41
SinI GGWCC 1 cut(s) 767
SlaI CTCGAG 1 cut(s) 602
SmiMI CAYNNNNRTG 4 cut(s) 332, 503, 669, 752
SmlI CTYRAG 4 cut(s) 182, 272, 419, 602
SmoI CTYRAG 4 cut(s) 182, 272, 419, 602
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 3 cut(s) 75, 93, 199
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 237, 430
TaqI TCGA 2 cut(s) 603, 762
TauI GCSGC 1 cut(s) 78
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 4 cut(s) 173, 176, 683, 782
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 5 cut(s) 17, 362, 371, 542, 680
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 767
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 602
XmnI GAANNNNTTC 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.