MD13G1281900.v1.1

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009985
Physical Location & Seq
Reverse (-)
41027647 .. 41029904
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1281900.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGCTCTCTCCGTCGAGAAAAGTAGCAGCGGCCGCGAGTACAAGGTCAAGGACATGTCTCAGGCCGACTTCGGCCGCCTCGAGATCGAGCTCGCTGAGGTCGAAATGCCCGGCCTCATCTCCTGCCGCACCGAATTCGGACCCTCCCAACCCTTAAAGGGGGCCCGCATCACCGGTTCCCTCCACATGACCATCCAAACTGCCGTCCTTATTGAAACCCTTACCGCCCTCGGCGCCGAGGTCCGCTGGTGCTCCTGCAACATTTTCTCCACCCAGGACCACGCTGCCGCCGCCATCGCCCGCGATAGCGCCGCCGTCTTCGCCTGGAAGGGCGAGACCCTCCAGGAGTACTGGTGGTGCACCGAGCGCGCCCTCGACTGGGGCCCCGGTGGCGGACCCGATCTGATCGTCGACGACGGCGGTGACGCCACCCTCTTGATCCACGAGGGAGTCAAGGCTGAGGAGGAGTTCGAGAAGTCCGGTACCCTCCCCGACCCATCTTCCACCGATAACCAGGAGTTCCAGCTGGTTCTGACCATCATCAGAGATGGCTTGAAGACCGACCCCAAGAGGTACCACAAGATGAAGGACAGGTTGGTCGGAGTTTCGGAGGAGACCACAACCGGCGTCAAGAGGTTGTATCAGATGCAGGCTAGCGGCGCTCTGTTGTTCCCTGCCATTAATGTCAATGATTCTGTTACAAAAAGCAAGTTTGACAACTTGTACGGATGCCGTCACTCTCTCCCTGATGGTTTGATGAGGGCCACGGATGTCATGATTGCCGGAAAGGTTTCCGTTGTCTGTGGATACGGAGATGTTGGAAAGGGTTGTGCTGCTGCCCTCAAGCAAGCAGGATCTCGTGTGATTGTGACTGAGATCGATCCAATTTGTGCCCTCCAGGCTCTTATGGAAGGCCTTCAGGTTCTTCCTCTTGAGGATGTTGTCTCTGAGGCTGATATCTTTGTTACCACCACCGGTAACAAGGACATCATCATGGTTGACCACATGAGGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGTCACTTTGACAATGAGATTGACATGCACGGTCTTGAGACATACCCCGGAGTGAAGCGCATCACCATTAAGCCTCAAACTGACAGGTGGGTCTTCCCAGAGACCAACACTGGCATCATTGTGTTGGCTGAGGGCCGTCTCATGAACTTGGGATGTGCCACTGGACACCCCAGCTTTGTGATGTCCTGCTCTTTCACCAACCAAGTGATTGCTCAGCTTGAGTTGTGGAACGAAAGGAAGTCTGGCAAGTACGAGAAGAAGGTGTATGTCTTGCCTAAGCACCTTGACGAGAAGGTTGCTGCCCTTCATCTTGGAAAGCTTGGAGCTAAGCTCACCAAGCTCAGCAAGGAACAAGCTGACTACATCAGTGTGCCGGTCGAGGGTCCATATAAGCCAGCTCACTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

486

Amino Acids

53.27

Weight (kDa)

5.72

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 13 - 484 1.4e-146 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 240 - 403 5.1e-83 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 259 - 349 9.9e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 596, 1133
Acc65I GGTACC 2 cut(s) 482, 573
AccB1I GGYRCC 3 cut(s) 233, 482, 573
AccI GTMKAC 1 cut(s) 411
AccII CGCG 3 cut(s) 36, 303, 369
AclWI GGATC 3 cut(s) 433, 862, 875
AcoI YGGCCR 2 cut(s) 31, 73
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 902
AcyI GRCGYC 3 cut(s) 234, 426, 627
AfaI GTAC 7 cut(s) 41, 350, 484, 575, 725, 1295, 1454
AfiI CCNNNNNNNGG 6 cut(s) 158, 159, 378, 379, 392, 1424
AflIII ACRYGT 1 cut(s) 54
AgeI ACCGGT 2 cut(s) 173, 974
AgsI TTSAA 2 cut(s) 215, 556
AjnI CCWGG 5 cut(s) 273, 323, 342, 513, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 3 cut(s) 93, 254, 362
Alw26I GTCTC 7 cut(s) 63, 329, 608, 949, 1076, 1139, 1187
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 3 cut(s) 433, 862, 875
AlwNI CAGNNNCTG 1 cut(s) 1455
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 9 cut(s) 31, 63, 73, 112, 162, 382, 762, 913, 1177
ApaI GGGCCC 2 cut(s) 166, 386
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 5 cut(s) 27, 284, 833, 836, 1343
ApoI RAATTY 1 cut(s) 134
AseI ATTAAT 1 cut(s) 681
AsiGI ACCGGT 2 cut(s) 173, 974
Asp700I GAANNNNTTC 2 cut(s) 790, 915
Asp718I GGTACC 2 cut(s) 482, 573
AspLEI GCGC 6 cut(s) 236, 311, 369, 371, 662, 1104
AsuC2I CCSGG 3 cut(s) 111, 387, 1092
AsuHPI GGTGA 5 cut(s) 163, 434, 1099, 1231, 1369
AsuNHI GCTAGC 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 5 cut(s) 140, 241, 277, 395, 1427
BaeGI GKGCMC 4 cut(s) 166, 362, 386, 895
BanI GGYRCC 3 cut(s) 233, 482, 573
BanII GRGCYC 3 cut(s) 93, 166, 386
BauI CACGAG 2 cut(s) 443, 858
BbsI GAAGAC 3 cut(s) 310, 563, 1129
Bbv12I GWGCWC 3 cut(s) 93, 254, 362
BbvCI CCTCAGC 3 cut(s) 96, 459, 1173
BbvI GCAGC 5 cut(s) 39, 271, 820, 823, 1330
BccI CCATC 6 cut(s) 200, 302, 505, 542, 545, 743
BceAI ACGGC 5 cut(s) 188, 299, 433, 717, 1164
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 1322, 1356
BciT130I CCWGG 5 cut(s) 275, 325, 344, 515, 899
BciVI GTATCC 1 cut(s) 800
BcnI CCSGG 3 cut(s) 111, 387, 1092
BcoDI GTCTC 7 cut(s) 63, 329, 608, 949, 1076, 1139, 1187
BfaI CTAG 1 cut(s) 654
BfmI CTRYAG 1 cut(s) 1447
BfoI RGCGCY 3 cut(s) 237, 312, 663
BfuI GTATCC 1 cut(s) 800
BglI GCCNNNNNGGC 2 cut(s) 390, 899
BlpI GCTNAGC 3 cut(s) 1257, 1371, 1385
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 8 cut(s) 111, 275, 325, 344, 387, 515, 899, 1092
Bme18I GGWCC 5 cut(s) 140, 241, 277, 395, 1427
BmeT110I CYCGRG 1 cut(s) 80
BmrFI CCNGG 8 cut(s) 111, 275, 325, 344, 387, 515, 899, 1092
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 5 cut(s) 177, 636, 719, 1113, 1167
BmtI GCTAGC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 3 cut(s) 310, 563, 1129
BplI GAGNNNNNCTC 2 cut(s) 1359, 1391
BpmI CTGGAG 2 cut(s) 326, 881
Bpu10I CCTNAGC 4 cut(s) 96, 459, 1173, 1320
Bpu1102I GCTNAGC 3 cut(s) 1257, 1371, 1385
BpuEI CTTGAG 4 cut(s) 827, 953, 1100, 1283
BpuMI CCSGG 3 cut(s) 111, 387, 1092
Bsa29I ATCGAT 1 cut(s) 879
BsaHI GRCGYC 3 cut(s) 234, 426, 627
BsaI GGTCTC 3 cut(s) 329, 608, 1139
BsaJI CCNNGG 6 cut(s) 229, 237, 273, 385, 765, 1090
BsaWI WCCGGW 3 cut(s) 173, 479, 974
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 338, 368
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 159, 378, 379, 392, 1424
Bse118I RCCGGY 4 cut(s) 173, 623, 974, 1417
Bse1I ACTGG 4 cut(s) 356, 383, 1159, 1210
BseBI CCWGG 5 cut(s) 275, 325, 344, 515, 899
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 6 cut(s) 229, 237, 273, 385, 765, 1090
BseGI GGATG 5 cut(s) 192, 734, 775, 943, 1202
BseLI CCNNNNNNNGG 6 cut(s) 158, 159, 378, 379, 392, 1424
BseMII CTCAG 8 cut(s) 74, 87, 450, 864, 939, 1164, 1271, 1399
BseNI ACTGG 4 cut(s) 356, 383, 1159, 1210
BsePI GCGCGC 1 cut(s) 367
BseRI GAGGAG 3 cut(s) 476, 479, 626
BseSI GKGCMC 4 cut(s) 166, 362, 386, 895
BseX3I CGGCCG 2 cut(s) 31, 73
BseXI GCAGC 5 cut(s) 39, 271, 820, 823, 1330
BseYI CCCAGC 1 cut(s) 1214
Bsh1236I CGCG 3 cut(s) 36, 303, 369
Bsh1285I CGRYCG 3 cut(s) 34, 76, 1422
BshFI GGCC 9 cut(s) 33, 65, 75, 114, 164, 384, 764, 915, 1179
BshNI GGYRCC 3 cut(s) 233, 482, 573
BshTI ACCGGT 2 cut(s) 173, 974
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 3 cut(s) 34, 76, 1422
BsiHKAI GWGCWC 3 cut(s) 93, 254, 362
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 9 cut(s) 111, 174, 387, 480, 624, 783, 975, 1092, 1418
BslI CCNNNNNNNGG 6 cut(s) 158, 159, 378, 379, 392, 1424
BsmAI GTCTC 7 cut(s) 63, 329, 608, 949, 1076, 1139, 1187
BsmBI CGTCTC 1 cut(s) 1187
BsnI GGCC 9 cut(s) 33, 65, 75, 114, 164, 384, 764, 915, 1179
Bso31I GGTCTC 3 cut(s) 329, 608, 1139
BsoBI CYCGRG 1 cut(s) 80
Bsp120I GGGCCC 2 cut(s) 162, 382
Bsp1286I GDGCHC 6 cut(s) 93, 166, 254, 362, 386, 895
Bsp143I GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
Bsp1720I GCTNAGC 3 cut(s) 1257, 1371, 1385
BspANI GGCC 9 cut(s) 33, 65, 75, 114, 164, 384, 764, 915, 1179
BspCNI CTCAG 8 cut(s) 73, 88, 451, 865, 940, 1165, 1270, 1398
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 3 cut(s) 36, 303, 369
BspHI TCATGA 2 cut(s) 774, 1185
BspOI GCTAGC 1 cut(s) 657
BspPI GGATC 3 cut(s) 433, 862, 875
BspT107I GGYRCC 3 cut(s) 233, 482, 573
BspTNI GGTCTC 3 cut(s) 329, 608, 1139
BsrFI RCCGGY 4 cut(s) 173, 623, 974, 1417
BsrI ACTGG 4 cut(s) 356, 383, 1159, 1210
BssAI RCCGGY 4 cut(s) 173, 623, 974, 1417
BssECI CCNNGG 6 cut(s) 229, 237, 273, 385, 765, 1090
BssHII GCGCGC 1 cut(s) 367
BssMI GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
BssNI GRCGYC 3 cut(s) 234, 426, 627
BssSI CACGAG 2 cut(s) 443, 858
Bst2BI CACGAG 2 cut(s) 443, 858
Bst2UI CCWGG 5 cut(s) 275, 325, 344, 515, 899
Bst4CI ACNGT 1 cut(s) 1076
BstACI GRCGYC 3 cut(s) 234, 426, 627
BstC8I GCNNGC 8 cut(s) 93, 166, 301, 369, 651, 655, 849, 1440
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 5 cut(s) 192, 734, 775, 943, 1202
BstFNI CGCG 3 cut(s) 36, 303, 369
BstH2I RGCGCY 3 cut(s) 237, 312, 663
BstHHI GCGC 6 cut(s) 236, 311, 369, 371, 662, 1104
BstKTI GATC 7 cut(s) 87, 403, 408, 441, 857, 879, 883
BstMAI GTCTC 7 cut(s) 63, 329, 608, 949, 1076, 1139, 1187
BstMBI GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
BstMCI CGRYCG 3 cut(s) 34, 76, 1422
BstNI CCWGG 5 cut(s) 275, 325, 344, 515, 899
BstNSI RCATGY 2 cut(s) 58, 1072
BstSCI CCNGG 8 cut(s) 109, 273, 323, 342, 385, 513, 897, 1090
BstSFI CTRYAG 1 cut(s) 1447
BstSLI GKGCMC 4 cut(s) 166, 362, 386, 895
BstUI CGCG 3 cut(s) 36, 303, 369
BstV1I GCAGC 5 cut(s) 39, 271, 820, 823, 1330
BstV2I GAAGAC 3 cut(s) 310, 563, 1129
BstX2I RGATCY 1 cut(s) 854
BstYI RGATCY 1 cut(s) 854
BstZI CGGCCG 2 cut(s) 31, 73
Bsu15I ATCGAT 1 cut(s) 879
BsuI GTATCC 1 cut(s) 800
BsuRI GGCC 9 cut(s) 33, 65, 75, 114, 164, 384, 764, 915, 1179
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 765
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 5 cut(s) 192, 734, 775, 943, 1202
BtsIMutI CAGTG 3 cut(s) 1152, 1203, 1417
Cac8I GCNNGC 8 cut(s) 93, 166, 301, 369, 651, 655, 849, 1440
CaiI CAGNNNCTG 1 cut(s) 1455
CciI TCATGA 2 cut(s) 774, 1185
CciNI GCGGCCGC 1 cut(s) 31
CfoI GCGC 6 cut(s) 236, 311, 369, 371, 662, 1104
Cfr10I RCCGGY 4 cut(s) 173, 623, 974, 1417
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 2 cut(s) 434, 616
Csp6I GTAC 7 cut(s) 40, 349, 483, 574, 724, 1294, 1453
CspAI ACCGGT 2 cut(s) 173, 974
CviAII CATG 7 cut(s) 55, 187, 775, 994, 1006, 1069, 1186
CviQI GTAC 7 cut(s) 40, 349, 483, 574, 724, 1294, 1453
DinI GGCGCC 1 cut(s) 235
DpnI GATC 7 cut(s) 86, 402, 407, 440, 856, 878, 882
DpnII GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
DrdI GACNNNNNNGTC 2 cut(s) 596, 1133
DseDI GACNNNNNNGTC 2 cut(s) 596, 1133
EaeI YGGCCR 2 cut(s) 31, 73
EagI CGGCCG 2 cut(s) 31, 73
EciI GGCGGA 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 91
EclXI CGGCCG 2 cut(s) 31, 73
Eco147I AGGCCT 1 cut(s) 915
Eco24I GRGCYC 3 cut(s) 93, 166, 386
Eco31I GGTCTC 3 cut(s) 329, 608, 1139
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 5 cut(s) 140, 241, 277, 395, 1427
Eco52I CGGCCG 2 cut(s) 31, 73
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 3 cut(s) 162, 382, 383
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 5 cut(s) 273, 323, 342, 513, 897
EcoRV GATATC 1 cut(s) 958
EcoT38I GRGCYC 3 cut(s) 93, 166, 386
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 1187
FaeI CATG 7 cut(s) 58, 190, 778, 997, 1009, 1072, 1189
FatI CATG 7 cut(s) 54, 186, 774, 993, 1005, 1068, 1185
FauI CCCGC 2 cut(s) 173, 308
FblI GTMKAC 1 cut(s) 411
FokI GGATG 5 cut(s) 179, 741, 782, 950, 1209
FriOI GRGCYC 3 cut(s) 93, 166, 386
FspBI CTAG 1 cut(s) 654
GlaI GCGC 6 cut(s) 235, 310, 368, 370, 661, 1103
GsaI CCCAGC 1 cut(s) 1218
GsuI CTGGAG 2 cut(s) 326, 881
HaeII RGCGCY 3 cut(s) 237, 312, 663
HaeIII GGCC 9 cut(s) 33, 65, 75, 114, 164, 384, 764, 915, 1179
HapII CCGG 9 cut(s) 111, 174, 387, 480, 624, 783, 975, 1092, 1418
HgaI GACGC 2 cut(s) 434, 616
HhaI GCGC 6 cut(s) 236, 311, 369, 371, 662, 1104
Hin1I GRCGYC 3 cut(s) 234, 426, 627
Hin1II CATG 7 cut(s) 58, 190, 778, 997, 1009, 1072, 1189
Hin6I GCGC 6 cut(s) 234, 309, 367, 369, 660, 1102
HinP1I GCGC 6 cut(s) 234, 309, 367, 369, 660, 1102
HincII GTYRAC 2 cut(s) 412, 1000
HindII GTYRAC 2 cut(s) 412, 1000
HindIII AAGCTT 1 cut(s) 1361
HinfI GANTC 2 cut(s) 450, 692
HpaII CCGG 9 cut(s) 111, 174, 387, 480, 624, 783, 975, 1092, 1418
HphI GGTGA 5 cut(s) 163, 434, 1099, 1231, 1369
Hpy166II GTNNAC 3 cut(s) 360, 412, 1000
Hpy188I TCNGA 8 cut(s) 140, 405, 534, 545, 602, 610, 645, 949
Hpy188III TCNNGA 9 cut(s) 16, 82, 436, 472, 631, 775, 932, 1079, 1186
Hpy8I GTNNAC 3 cut(s) 360, 412, 1000
Hpy99I CGWCG 4 cut(s) 17, 413, 416, 419
HpyAV CCTTC 7 cut(s) 322, 580, 905, 926, 1297, 1330, 1358
HpyCH4III ACNGT 1 cut(s) 1076
HpyCH4V TGCA 5 cut(s) 258, 360, 649, 1038, 1072
Hsp92I GRCGYC 3 cut(s) 234, 426, 627
Hsp92II CATG 7 cut(s) 58, 190, 778, 997, 1009, 1072, 1189
HspAI GCGC 6 cut(s) 234, 309, 367, 369, 660, 1102
KasI GGCGCC 1 cut(s) 233
KpnI GGTACC 2 cut(s) 486, 577
Kzo9I GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
LmnI GCTCC 2 cut(s) 257, 1367
Lsp1109I GCAGC 5 cut(s) 39, 271, 820, 823, 1330
LweI GCATC 5 cut(s) 177, 636, 719, 1113, 1167
MaeI CTAG 1 cut(s) 654
MaeIII GTNAC 7 cut(s) 422, 697, 734, 868, 964, 977, 1046
MalI GATC 7 cut(s) 86, 402, 407, 440, 856, 878, 882
MboI GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
MboII GAAGA 8 cut(s) 310, 492, 568, 917, 1024, 1030, 1129, 1312
MflI RGATCY 1 cut(s) 854
MhlI GDGCHC 6 cut(s) 93, 166, 254, 362, 386, 895
MluCI AATT 2 cut(s) 134, 885
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 1 cut(s) 459
MmeI TCCRAC 2 cut(s) 580, 799
MroXI GAANNNNTTC 2 cut(s) 790, 915
MseI TTAA 3 cut(s) 155, 681, 1113
MslI CAYNNNNRTG 3 cut(s) 992, 1163, 1412
MspA1I CMGCKG 3 cut(s) 30, 246, 526
MspI CCGG 9 cut(s) 111, 174, 387, 480, 624, 783, 975, 1092, 1418
MspR9I CCNGG 8 cut(s) 111, 275, 325, 344, 387, 515, 899, 1092
MvaI CCWGG 5 cut(s) 275, 325, 344, 515, 899
MvnI CGCG 3 cut(s) 36, 303, 369
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 3 cut(s) 111, 387, 1092
NdeII GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
NheI GCTAGC 1 cut(s) 653
NlaIII CATG 7 cut(s) 58, 190, 778, 997, 1009, 1072, 1189
NmeAIII GCCGAG 2 cut(s) 210, 262
NmuCI GTSAC 4 cut(s) 422, 734, 868, 1046
NotI GCGGCCGC 1 cut(s) 31
NspI RCATGY 2 cut(s) 58, 1072
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 2 cut(s) 774, 1185
PauI GCGCGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 915
PciI ACATGT 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 99
PdmI GAANNNNTTC 2 cut(s) 790, 915
PfeI GAWTC 1 cut(s) 692
PfoI TCCNGGA 1 cut(s) 342
PinAI ACCGGT 2 cut(s) 173, 974
PleI GAGTC 1 cut(s) 458
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 458
PscI ACATGT 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 5 cut(s) 273, 323, 342, 513, 897
PspFI CCCAGC 1 cut(s) 1214
PspGI CCWGG 5 cut(s) 273, 323, 342, 513, 897
PspOMI GGGCCC 2 cut(s) 162, 382
PstNI CAGNNNCTG 1 cut(s) 1455
PsuI RGATCY 1 cut(s) 854
PteI GCGCGC 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 7 cut(s) 41, 350, 484, 575, 725, 1295, 1454
RsaNI GTAC 7 cut(s) 40, 349, 483, 574, 724, 1294, 1453
RseI CAYNNNNRTG 3 cut(s) 992, 1163, 1412
SacI GAGCTC 1 cut(s) 93
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 3 cut(s) 155, 681, 1113
Sau3AI GATC 7 cut(s) 84, 400, 405, 438, 854, 876, 880
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 8 cut(s) 111, 275, 325, 344, 387, 515, 899, 1092
SduI GDGCHC 6 cut(s) 93, 166, 254, 362, 386, 895
SfaNI GCATC 5 cut(s) 177, 636, 719, 1113, 1167
SfcI CTRYAG 1 cut(s) 1447
SfoI GGCGCC 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 80
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 5 cut(s) 140, 241, 277, 395, 1427
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 992, 1163, 1412
SmlI CTYRAG 5 cut(s) 80, 842, 932, 1079, 1262
SmoI CTYRAG 5 cut(s) 80, 842, 932, 1079, 1262
Sse9I AATT 2 cut(s) 134, 885
SseBI AGGCCT 1 cut(s) 915
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 1 cut(s) 654
SstI GAGCTC 1 cut(s) 93
StuI AGGCCT 1 cut(s) 915
StyD4I CCNGG 8 cut(s) 109, 273, 323, 342, 385, 513, 897, 1090
TaaI ACNGT 1 cut(s) 1076
TaqI TCGA 9 cut(s) 15, 81, 87, 102, 375, 411, 471, 879, 1422
TaqII GACCGA 1 cut(s) 575
TasI AATT 2 cut(s) 134, 885
TatI WGTACW 2 cut(s) 39, 348
TauI GCSGC 8 cut(s) 33, 36, 78, 129, 290, 293, 314, 660
TfiI GAWTC 1 cut(s) 692
Tru1I TTAA 3 cut(s) 155, 681, 1113
Tru9I TTAA 3 cut(s) 155, 681, 1113
TscAI CASTG 3 cut(s) 1159, 1210, 1417
TseFI GTSAC 4 cut(s) 422, 734, 868, 1046
TseI GCWGC 5 cut(s) 27, 284, 833, 836, 1343
Tsp45I GTSAC 4 cut(s) 422, 734, 868, 1046
TspDTI ATGAA 4 cut(s) 599, 1031, 1202, 1340
TspGWI ACGGA 4 cut(s) 741, 782, 784, 825
TspRI CASTG 3 cut(s) 1159, 1210, 1417
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 5 cut(s) 140, 241, 277, 395, 1427
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 58, 1072
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 2 cut(s) 790, 915
XspI CTAG 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.