FvH4_4g13280

adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009985
Physical Location & Seq
Reverse (-)
16864345 .. 16867553
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13280.t1

Sequence Viewer

Length: 1458 bp
ATGGCTCTCTCCGTCGAGAAGCATAGCTCCGGCCGCGAGTACAAGGTCAAGGACATGTCCCAGGCCGACTTCGGCAGGCTCGAGATCGAGCTCGCCGAGGTCGAAATGCCTGGTCTCATGGCCTCCCGTGTCGAATTCGGCCCGTCCCAGCCCTTCAAGGGTGCCCGGATCACCGGATCTCTCCACATGACCATCCAGACCGCCGTCCTCATCGAAACCCTAACCGCTCTCGGAGCCGAGGTCCGCTGGTGCTCCTGCAACATCTTCTCCACCCAGGACCACGCCGCCGCCGCCATTGCTCGCGATAGCGCCGCTGTTTTCGCCTGGAAGGGAGAGACTCTCCAGGAGTACTGGTGGTGCACTGAGCGCGCCTTGGACTGGGGCCCCGGCGGAGGCCCAGATCTGATCGTCGACGACGGTGGTGACACTACTCTCTTGATCCATGAGGGTGTTAAGGCCGAGGAGATCTTTGAGAAGACCGGGCAGCTACCTGACCCGACTTCCACCGACAACGCGGAGTTCCAGATCGTGCTCTCTATTATCAAGGAGGGGTTGAAGACCGACCCCAAGAGGTACCGCAAGATGAAGGACAGGATTGTCGGTGTCTCGGAGGAGACCACAACCGGAGTCAAGAGGCTGTACCAGATGCAGAACAGCGGCTCATTGTTGTTCCCTGCTATCAATGTCAATGACTCTGTCACCAAGAGCAAGTTTGACAACTTGTATGGTTGCCGTCACTCTCTCCCTGATGGTTTGATGAGAGCCACTGATGTTATGATTGCCGGAAAGGTTTCTGTTGTCTGTGGATATGGTGATGTTGGAAAGGGTTGTGCTGCTGCTCTCAAGCAAGCCGGATCCCGTGTGATCGTGACTGAGATTGACCCAATCTGTGCTCTTCAGGCTCTTATGGAAGGCCTTCAGGTTCTTCCCATTGAGGACGTCGTCTCTGATGCTGATATCTTTGTCACCACCACCGGAAACAAGGACATCATCATGCTTGAGCACATGAGGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGGCATTTTGACAATGAGATCGACATGCATGGACTTGAGACATTCCCAGGAGTGCAGCGTATCACCATCAAGCCTCAAACAGATAGGTGGGTTTTCCCCGATACCAAGACTGGTATCATTGTGTTGGCTGAGGGCCGTCTCATGAACTTGGGTTGTGCCACTGGACACCCTAGCTTTGTGATGTCCTGCTCTTTCACCAACCAAGTGATTGCTCAGCTTGAGTTGTGGAATGAGAGGAAGAGCGGCAAGTATGAGAAGAAGGTCTATGTCTTGCCTAAGCACCTTGATGAGAAGGTTGCTGCTCTTCATCTTGGAAAGCTCGGAGCCAAGCTCACCAAGCTCAGCAAGGAGCAGGCTGACTATATCAGCGTTCCAATTGAGGGTCCATACAAGCCTGCTCACTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

486

Amino Acids

53.42

Weight (kDa)

5.9

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 13 - 484 6e-146 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 240 - 403 9.8e-83 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 259 - 349 4.2e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 596
AatII GACGTC 1 cut(s) 942
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 2 cut(s) 161, 573
AccBSI CCGCTC 2 cut(s) 227, 1287
AccI GTMKAC 1 cut(s) 411
AccII CGCG 4 cut(s) 36, 303, 369, 515
AclWI GGATC 5 cut(s) 176, 184, 433, 849, 862
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 2 cut(s) 881, 902
AcyI GRCGYC 1 cut(s) 939
AfaI GTAC 5 cut(s) 41, 350, 575, 641, 1454
AfiI CCNNNNNNNGG 4 cut(s) 158, 378, 392, 1424
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 2 cut(s) 157, 556
AjnI CCWGG 6 cut(s) 60, 109, 273, 323, 342, 1090
AluBI AGCT 8 cut(s) 27, 91, 487, 1218, 1261, 1363, 1375, 1384
AluI AGCT 8 cut(s) 27, 91, 487, 1218, 1261, 1363, 1375, 1384
Alw21I GWGCWC 6 cut(s) 93, 254, 362, 534, 895, 1005
Alw26I GTCTC 7 cut(s) 119, 329, 608, 610, 949, 1076, 1187
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 5 cut(s) 176, 184, 433, 849, 862
AlwNI CAGNNNCTG 1 cut(s) 1455
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 9 cut(s) 31, 63, 120, 139, 382, 394, 456, 913, 1177
ApaI GGGCCC 1 cut(s) 386
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 5 cut(s) 484, 833, 836, 1099, 1343
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 2 cut(s) 790, 915
Asp718I GGTACC 1 cut(s) 573
AspLEI GCGC 3 cut(s) 311, 369, 371
AspS9I GGNCC 8 cut(s) 140, 241, 277, 382, 383, 395, 1177, 1427
AsuC2I CCSGG 3 cut(s) 166, 387, 481
AsuHPI GGTGA 8 cut(s) 163, 434, 691, 824, 958, 1099, 1231, 1369
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 3 cut(s) 241, 277, 1427
BaeGI GKGCMC 3 cut(s) 166, 362, 386
BamHI GGATCC 1 cut(s) 854
BanI GGYRCC 2 cut(s) 161, 573
BanII GRGCYC 2 cut(s) 93, 386
BbsI GAAGAC 2 cut(s) 482, 563
Bbv12I GWGCWC 6 cut(s) 93, 254, 362, 534, 895, 1005
BbvCI CCTCAGC 1 cut(s) 1173
BbvI GCAGC 5 cut(s) 496, 820, 823, 1111, 1330
BccI CCATC 3 cut(s) 200, 743, 1118
BceAI ACGGC 3 cut(s) 188, 717, 1164
BciT130I CCWGG 6 cut(s) 62, 111, 275, 325, 344, 1092
BcnI CCSGG 3 cut(s) 166, 387, 481
BcoDI GTCTC 7 cut(s) 119, 329, 608, 610, 949, 1076, 1187
BfaI CTAG 1 cut(s) 1215
BfmI CTRYAG 1 cut(s) 1447
BfoI RGCGCY 1 cut(s) 312
BglII AGATCT 2 cut(s) 400, 465
BlpI GCTNAGC 2 cut(s) 1257, 1385
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 9 cut(s) 62, 111, 166, 275, 325, 344, 387, 481, 1092
Bme18I GGWCC 3 cut(s) 241, 277, 1427
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 8 cut(s) 140, 241, 277, 382, 383, 395, 1177, 1427
BmiI GGNNCC 9 cut(s) 163, 235, 383, 384, 385, 575, 856, 1369, 1428
BmrFI CCNGG 9 cut(s) 62, 111, 166, 275, 325, 344, 387, 481, 1092
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 2 cut(s) 636, 940
BmuI ACTGGG 1 cut(s) 388
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 2 cut(s) 482, 563
BplI GAGNNNNNCTC 4 cut(s) 324, 356, 1359, 1391
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 2 cut(s) 1173, 1320
Bpu1102I GCTNAGC 2 cut(s) 1257, 1385
BpuEI CTTGAG 4 cut(s) 827, 1019, 1100, 1283
BpuMI CCSGG 3 cut(s) 166, 387, 481
BsaHI GRCGYC 1 cut(s) 939
BsaI GGTCTC 2 cut(s) 119, 608
BsaJI CCNNGG 8 cut(s) 60, 96, 237, 273, 372, 385, 459, 1090
BsaWI WCCGGW 3 cut(s) 173, 623, 974
BsaXI ACNNNNNCTCC 6 cut(s) 225, 251, 255, 281, 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 378, 392, 1424
Bse1I ACTGG 4 cut(s) 356, 383, 1159, 1210
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 6 cut(s) 62, 111, 275, 325, 344, 1092
BseDI CCNNGG 8 cut(s) 60, 96, 237, 273, 372, 385, 459, 1090
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 4 cut(s) 158, 378, 392, 1424
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 5 cut(s) 354, 864, 1164, 1271, 1399
BseNI ACTGG 4 cut(s) 356, 383, 1159, 1210
BsePI GCGCGC 1 cut(s) 367
BseRI GAGGAG 2 cut(s) 476, 626
BseSI GKGCMC 3 cut(s) 166, 362, 386
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 5 cut(s) 496, 820, 823, 1111, 1330
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 1118
Bsh1236I CGCG 4 cut(s) 36, 303, 369, 515
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 9 cut(s) 33, 65, 122, 141, 384, 396, 458, 915, 1179
BshNI GGYRCC 2 cut(s) 161, 573
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 6 cut(s) 93, 254, 362, 534, 895, 1005
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 9 cut(s) 30, 166, 174, 387, 480, 624, 783, 852, 975
BslFI GGGAC 2 cut(s) 43, 130
BslI CCNNNNNNNGG 4 cut(s) 158, 378, 392, 1424
BsmAI GTCTC 7 cut(s) 119, 329, 608, 610, 949, 1076, 1187
BsmBI CGTCTC 2 cut(s) 949, 1187
BsmFI GGGAC 2 cut(s) 43, 130
BsnI GGCC 9 cut(s) 33, 65, 122, 141, 384, 396, 458, 915, 1179
Bso31I GGTCTC 2 cut(s) 119, 608
BsoBI CYCGRG 1 cut(s) 80
Bsp120I GGGCCC 1 cut(s) 382
Bsp1286I GDGCHC 8 cut(s) 93, 166, 254, 362, 386, 534, 895, 1005
Bsp1720I GCTNAGC 2 cut(s) 1257, 1385
Bsp68I TCGCGA 1 cut(s) 303
BspANI GGCC 9 cut(s) 33, 65, 122, 141, 384, 396, 458, 915, 1179
BspCNI CTCAG 5 cut(s) 355, 865, 1165, 1270, 1398
BspFNI CGCG 4 cut(s) 36, 303, 369, 515
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 9 cut(s) 163, 235, 383, 384, 385, 575, 856, 1369, 1428
BspPI GGATC 5 cut(s) 176, 184, 433, 849, 862
BspQI GCTCTTC 3 cut(s) 900, 1277, 1353
BspT107I GGYRCC 2 cut(s) 161, 573
BspTNI GGTCTC 2 cut(s) 119, 608
BsrBI CCGCTC 2 cut(s) 227, 1287
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 4 cut(s) 356, 383, 1159, 1210
BssECI CCNNGG 8 cut(s) 60, 96, 237, 273, 372, 385, 459, 1090
BssHII GCGCGC 1 cut(s) 367
BssNI GRCGYC 1 cut(s) 939
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 6 cut(s) 62, 111, 275, 325, 344, 1092
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 3 cut(s) 900, 1277, 1353
BstACI GRCGYC 1 cut(s) 939
BstC8I GCNNGC 7 cut(s) 77, 93, 301, 369, 849, 1398, 1440
BstDEI CTNAG 6 cut(s) 363, 873, 1173, 1257, 1320, 1385
BstF5I GGATG 1 cut(s) 192
BstFNI CGCG 4 cut(s) 36, 303, 369, 515
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 3 cut(s) 311, 369, 371
BstMAI GTCTC 7 cut(s) 119, 329, 608, 610, 949, 1076, 1187
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 8 cut(s) 33, 233, 290, 296, 320, 366, 899, 1381
BstNI CCWGG 6 cut(s) 62, 111, 275, 325, 344, 1092
BstNSI RCATGY 2 cut(s) 58, 1072
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 9 cut(s) 60, 109, 164, 273, 323, 342, 385, 479, 1090
BstSFI CTRYAG 1 cut(s) 1447
BstSLI GKGCMC 3 cut(s) 166, 362, 386
BstUI CGCG 4 cut(s) 36, 303, 369, 515
BstV1I GCAGC 5 cut(s) 496, 820, 823, 1111, 1330
BstV2I GAAGAC 2 cut(s) 482, 563
BstX2I RGATCY 4 cut(s) 176, 400, 465, 854
BstYI RGATCY 4 cut(s) 176, 400, 465, 854
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 9 cut(s) 33, 65, 122, 141, 384, 396, 458, 915, 1179
BtsCI GGATG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 360, 765, 1203
BtuMI TCGCGA 1 cut(s) 303
Cac8I GCNNGC 7 cut(s) 77, 93, 301, 369, 849, 1398, 1440
CaiI CAGNNNCTG 1 cut(s) 1455
CciI TCATGA 1 cut(s) 1185
CfoI GCGC 3 cut(s) 311, 369, 371
Cfr13I GGNCC 8 cut(s) 140, 241, 277, 382, 383, 395, 1177, 1427
Csp6I GTAC 5 cut(s) 40, 349, 574, 640, 1453
CviAII CATG 9 cut(s) 55, 118, 187, 443, 994, 1006, 1069, 1073, 1186
CviQI GTAC 5 cut(s) 40, 349, 574, 640, 1453
DdeI CTNAG 6 cut(s) 363, 873, 1173, 1257, 1320, 1385
DrdI GACNNNNNNGTC 1 cut(s) 596
DseDI GACNNNNNNGTC 1 cut(s) 596
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 900, 1277, 1353
EarI CTCTTC 3 cut(s) 900, 1277, 1353
EciI GGCGGA 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 91
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 915
Eco24I GRGCYC 2 cut(s) 93, 386
Eco31I GGTCTC 2 cut(s) 119, 608
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 3 cut(s) 241, 277, 1427
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 2 cut(s) 881, 902
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 2 cut(s) 382, 383
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 6 cut(s) 60, 109, 273, 323, 342, 1090
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 1074
EcoT38I GRGCYC 2 cut(s) 93, 386
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 2 cut(s) 949, 1187
FaeI CATG 9 cut(s) 58, 121, 190, 446, 997, 1009, 1072, 1076, 1189
FaqI GGGAC 2 cut(s) 43, 130
FatI CATG 9 cut(s) 54, 117, 186, 442, 993, 1005, 1068, 1072, 1185
FblI GTMKAC 1 cut(s) 411
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 93, 386
FspBI CTAG 1 cut(s) 1215
GlaI GCGC 3 cut(s) 310, 368, 370
GsaI CCCAGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 9 cut(s) 33, 65, 122, 141, 384, 396, 458, 915, 1179
HapII CCGG 9 cut(s) 30, 166, 174, 387, 480, 624, 783, 852, 975
HhaI GCGC 3 cut(s) 311, 369, 371
Hin1I GRCGYC 1 cut(s) 939
Hin1II CATG 9 cut(s) 58, 121, 190, 446, 997, 1009, 1072, 1076, 1189
Hin6I GCGC 3 cut(s) 309, 367, 369
HinP1I GCGC 3 cut(s) 309, 367, 369
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 3 cut(s) 337, 627, 692
HpaII CCGG 9 cut(s) 30, 166, 174, 387, 480, 624, 783, 852, 975
HphI GGTGA 8 cut(s) 163, 434, 691, 824, 958, 1099, 1231, 1369
Hpy166II GTNNAC 2 cut(s) 360, 412
Hpy188I TCNGA 5 cut(s) 233, 405, 610, 949, 1367
Hpy188III TCNNGA 9 cut(s) 16, 82, 196, 302, 436, 523, 631, 868, 1186
Hpy8I GTNNAC 2 cut(s) 360, 412
Hpy99I CGWCG 5 cut(s) 17, 413, 416, 419, 944
HpyAV CCTTC 7 cut(s) 163, 322, 580, 905, 926, 1297, 1330
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 6 cut(s) 258, 360, 649, 1038, 1072, 1099
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 233, 290, 296, 320, 366, 899, 1381
HpyF3I CTNAG 6 cut(s) 363, 873, 1173, 1257, 1320, 1385
HpySE526I ACGT 1 cut(s) 939
Hsp92I GRCGYC 1 cut(s) 939
Hsp92II CATG 9 cut(s) 58, 121, 190, 446, 997, 1009, 1072, 1076, 1189
HspAI GCGC 3 cut(s) 309, 367, 369
KpnI GGTACC 1 cut(s) 577
LguI GCTCTTC 3 cut(s) 900, 1277, 1353
LmnI GCTCC 5 cut(s) 32, 233, 257, 1367, 1393
Lsp1109I GCAGC 5 cut(s) 496, 820, 823, 1111, 1330
LweI GCATC 2 cut(s) 636, 940
MaeI CTAG 1 cut(s) 1215
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 5 cut(s) 422, 697, 734, 868, 964
MbiI CCGCTC 2 cut(s) 227, 1287
MfeI CAATTG 1 cut(s) 1419
MflI RGATCY 4 cut(s) 176, 400, 465, 854
MhlI GDGCHC 8 cut(s) 93, 166, 254, 362, 386, 534, 895, 1005
MluCI AATT 2 cut(s) 134, 1419
MlyI GAGTC 3 cut(s) 331, 636, 686
MmeI TCCRAC 1 cut(s) 799
Mph1103I ATGCAT 1 cut(s) 1074
MroXI GAANNNNTTC 2 cut(s) 790, 915
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 3 cut(s) 447, 992, 1329
MspA1I CMGCKG 3 cut(s) 246, 314, 657
MspI CCGG 9 cut(s) 30, 166, 174, 387, 480, 624, 783, 852, 975
MspR9I CCNGG 9 cut(s) 62, 111, 166, 275, 325, 344, 387, 481, 1092
MunI CAATTG 1 cut(s) 1419
MvaI CCWGG 6 cut(s) 62, 111, 275, 325, 344, 1092
MvnI CGCG 4 cut(s) 36, 303, 369, 515
MwoI GCNNNNNNNGC 8 cut(s) 33, 233, 290, 296, 320, 366, 899, 1381
NciI CCSGG 3 cut(s) 166, 387, 481
NlaIII CATG 9 cut(s) 58, 121, 190, 446, 997, 1009, 1072, 1076, 1189
NlaIV GGNNCC 9 cut(s) 163, 235, 383, 384, 385, 575, 856, 1369, 1428
NmeAIII GCCGAG 3 cut(s) 121, 262, 484
NmuCI GTSAC 5 cut(s) 422, 697, 734, 868, 964
NruI TCGCGA 1 cut(s) 303
NsiI ATGCAT 1 cut(s) 1074
NspI RCATGY 2 cut(s) 58, 1072
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 1185
PauI GCGCGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 915
PciI ACATGT 1 cut(s) 54
PciSI GCTCTTC 3 cut(s) 900, 1277, 1353
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 93, 99
PdmI GAANNNNTTC 2 cut(s) 790, 915
PflFI GACNNNGTC 2 cut(s) 695, 941
PfoI TCCNGGA 1 cut(s) 342
PleI GAGTC 3 cut(s) 331, 635, 686
PpsI GAGTC 3 cut(s) 331, 635, 686
PscI ACATGT 1 cut(s) 54
PshAI GACNNNNGTC 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 6 cut(s) 60, 109, 273, 323, 342, 1090
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 6 cut(s) 60, 109, 273, 323, 342, 1090
PspN4I GGNNCC 9 cut(s) 163, 235, 383, 384, 385, 575, 856, 1369, 1428
PspOMI GGGCCC 1 cut(s) 382
PspPI GGNCC 8 cut(s) 140, 241, 277, 382, 383, 395, 1177, 1427
PstNI CAGNNNCTG 1 cut(s) 1455
PsuI RGATCY 4 cut(s) 176, 400, 465, 854
PsyI GACNNNGTC 2 cut(s) 695, 941
PteI GCGCGC 1 cut(s) 367
RruI TCGCGA 1 cut(s) 303
RsaI GTAC 5 cut(s) 41, 350, 575, 641, 1454
RsaNI GTAC 5 cut(s) 40, 349, 574, 640, 1453
RseI CAYNNNNRTG 3 cut(s) 447, 992, 1329
SacI GAGCTC 1 cut(s) 93
SalI GTCGAC 1 cut(s) 410
SapI GCTCTTC 3 cut(s) 900, 1277, 1353
SaqAI TTAA 1 cut(s) 453
Sau96I GGNCC 8 cut(s) 140, 241, 277, 382, 383, 395, 1177, 1427
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 331, 636, 686
ScrFI CCNGG 9 cut(s) 62, 111, 166, 275, 325, 344, 387, 481, 1092
SduI GDGCHC 8 cut(s) 93, 166, 254, 362, 386, 534, 895, 1005
SfaNI GCATC 2 cut(s) 636, 940
SfcI CTRYAG 1 cut(s) 1447
Sfr274I CTCGAG 1 cut(s) 80
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 3 cut(s) 241, 277, 1427
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 447, 992, 1329
SmlI CTYRAG 5 cut(s) 80, 842, 998, 1079, 1262
SmoI CTYRAG 5 cut(s) 80, 842, 998, 1079, 1262
Sse9I AATT 2 cut(s) 134, 1419
SseBI AGGCCT 1 cut(s) 915
SspMI CTAG 1 cut(s) 1215
SstI GAGCTC 1 cut(s) 93
StuI AGGCCT 1 cut(s) 915
StyD4I CCNGG 9 cut(s) 60, 109, 164, 273, 323, 342, 385, 479, 1090
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 1 cut(s) 942
TaqI TCGA 8 cut(s) 15, 81, 87, 102, 132, 213, 411, 1065
TaqII GACCGA 1 cut(s) 575
TasI AATT 2 cut(s) 134, 1419
TatI WGTACW 2 cut(s) 39, 348
TauI GCSGC 7 cut(s) 36, 287, 290, 293, 314, 660, 1290
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 367, 772, 1210
TseFI GTSAC 5 cut(s) 422, 697, 734, 868, 964
TseI GCWGC 5 cut(s) 484, 833, 836, 1099, 1343
Tsp45I GTSAC 5 cut(s) 422, 697, 734, 868, 964
TspDTI ATGAA 4 cut(s) 599, 1031, 1202, 1340
TspRI CASTG 3 cut(s) 367, 772, 1210
Tth111I GACNNNGTC 2 cut(s) 695, 941
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 3 cut(s) 241, 277, 1427
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 58, 1072
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 2 cut(s) 790, 915
XspI CTAG 1 cut(s) 1215
ZraI GACGTC 1 cut(s) 940
ZrmI AGTACT 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.