Rh4BG179900

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009985
Physical Location & Seq
Reverse (-)
31780668 .. 31784068
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG179900.1

Sequence Viewer

Length: 1458 bp
ATGGCTCTCTGCGTCGAGAAAAACAGCTCCGGCCGCGAGTACAAGGTCAAGGACATGTCCCAGGCCGACTTCGGCAGGCTCGAGATCGAGCTCGCCGAGGTCGAAATGCCCGGTCTCATGGCCTCCCGGGCCGAATTCGGCCCGGCCCAGCCCTTCAAGGGAGCCCGCATCACCGGATCCCTCCACATGACCATCCAGACCGCCGTCCTCATCGAAACCCTAACCGCCCTCGGCGCCGAGGTCCGCTGGTGCTCCTGCAACATCTTCTCCACTCAGGACCACGCCGCCGCCGCCATTGCCCGCGACAGCGCCGCCGTCTTCGCCTGGAAGGGCGAGACCCTCCAGGAGTACTGGTGGTGCACCGAGCGCGCTCTTGACTGGGGCCCCGGCGGAGGCCCAGATCTGATCGTCGACGACGGCGGTGACGCCACTCTTCTGATCCACGAGGGTGTCAAGGCCGAGGAGCTCTTTGAGAAGACCGGGCAGCTACCCGACCCGTCTTCCACCGATAACCAGGAGTTCCAGCTGGTTCTCACCATCATCAGAGATGGCTTGAAGACCGACCCCAAGAGGTACCACAAGATGAAGGACAGGTTGGTTGGTGTTTCCGAGGAGACCACCACCGGAGTCAAGAGGCTCTACCAGATGCAGGCCAGTGGCACATTGTTGTTCCCTGCTATCAATGTCAATGACTCTGTTACCAAGAGCAAGTTTGACAACTTGTATGGTTGCCGTCACTCTCTCCCTGATGGTTTGATGAGAGCCACTGATGTTATGATTGCCGGAAAGGTTTCTGTTGTCTGTGGATATGGTGATGTTGGAAAGGGTTGTGCTGCTGCCCTCAAGCAAGCTGGATCCCGTGTGATCGTGACTGAGATTGACCCAATCTGTGCTCTTCAGGCTACTATGGAGGGTCTTCAGGTTCTTCCCCTTGAGGACGTTGTCTCTGAGGCTGACATCTTTGTCACCACCACCGGAAACAAGGACATCATCATGGTTGACCAGATGAGGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGGCATTTTGACAATGAGATCGACATGCTTGGACTTGAGACATACCCAGGAGTGAAGCGCATCACCATCAAGCCTCAAACAGACAGGTGGGTCTTCCCTGAGACCAAGTCTGGTATCATTGTGTTGGCTGAGGGCCGTCTCATGAACTTGGGTTGTGCCACTGGACACCCCAGCTTTGTGATGTCCTGCTCTTTCACCAACCAAGTGATTGCTCAGCTTGAGTTGTGGAATGAGAGGAAGAGCGGCAAGTATGAGAAGAAGGTCTATGTCTTGCCCAAGCACCTTGATGAGAAGGTTGCTGCTCTTCATCTTGGAAAGCTTGGAGCCAAGCTCACCAAGCTCACCAAGGACCAAGCCGACTACATCAACGTTCCAATTGAGGGTCCATACAAGCCTGCTCAGTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

485

Amino Acids

53.33

Weight (kDa)

5.72

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 13 - 484 6.8e-146 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 240 - 403 2.7e-82 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 259 - 349 3.2e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 962, 1133
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 2 cut(s) 233, 573
AccBSI CCGCTC 1 cut(s) 1287
AccI GTMKAC 1 cut(s) 411
AccII CGCG 3 cut(s) 36, 303, 369
AclI AACGTT 1 cut(s) 1413
AclWI GGATC 5 cut(s) 171, 184, 433, 849, 862
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 2 cut(s) 881, 902
AcyI GRCGYC 2 cut(s) 234, 426
AfaI GTAC 5 cut(s) 41, 350, 575, 1448, 1454
AfiI CCNNNNNNNGG 4 cut(s) 158, 392, 649, 1424
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 2 cut(s) 157, 556
AjnI CCWGG 5 cut(s) 60, 323, 342, 513, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AleI CACNNNNGTG 1 cut(s) 447
Alw21I GWGCWC 5 cut(s) 93, 254, 362, 468, 895
Alw26I GTCTC 7 cut(s) 119, 329, 608, 949, 1076, 1139, 1187
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 5 cut(s) 171, 184, 433, 849, 862
AlwNI CAGNNNCTG 1 cut(s) 1455
Ama87I CYCGRG 2 cut(s) 80, 126
ApaI GGGCCC 1 cut(s) 386
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 4 cut(s) 484, 833, 836, 1343
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 790
Asp718I GGTACC 1 cut(s) 573
AspLEI GCGC 5 cut(s) 236, 311, 369, 371, 1104
AsuC2I CCSGG 6 cut(s) 111, 127, 128, 143, 387, 481
AsuHPI GGTGA 9 cut(s) 163, 434, 526, 824, 958, 1099, 1231, 1369, 1378
AvaI CYCGRG 2 cut(s) 80, 126
AvaII GGWCC 4 cut(s) 241, 277, 1393, 1427
BaeGI GKGCMC 2 cut(s) 362, 386
BamHI GGATCC 2 cut(s) 176, 854
BanI GGYRCC 2 cut(s) 233, 573
BanII GRGCYC 4 cut(s) 93, 166, 386, 468
BauI CACGAG 1 cut(s) 443
BbsI GAAGAC 6 cut(s) 310, 482, 492, 563, 908, 1129
Bbv12I GWGCWC 5 cut(s) 93, 254, 362, 468, 895
BbvCI CCTCAGC 1 cut(s) 1173
BbvI GCAGC 4 cut(s) 496, 820, 823, 1330
BccI CCATC 5 cut(s) 200, 542, 545, 743, 1118
BceAI ACGGC 5 cut(s) 188, 299, 433, 717, 1164
BciT130I CCWGG 5 cut(s) 62, 325, 344, 515, 1092
BcnI CCSGG 6 cut(s) 111, 127, 128, 143, 387, 481
BcoDI GTCTC 7 cut(s) 119, 329, 608, 949, 1076, 1139, 1187
BfoI RGCGCY 2 cut(s) 237, 312
BglI GCCNNNNNGGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 400
BlpI GCTNAGC 1 cut(s) 1257
BmcAI AGTACT 1 cut(s) 350
Bme18I GGWCC 4 cut(s) 241, 277, 1393, 1427
BmeT110I CYCGRG 2 cut(s) 80, 126
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 3 cut(s) 177, 636, 1113
BmuI ACTGGG 1 cut(s) 388
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 6 cut(s) 310, 482, 492, 563, 908, 1129
BplI GAGNNNNNCTC 2 cut(s) 1359, 1391
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 1173
Bpu1102I GCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 4 cut(s) 827, 953, 1100, 1283
BpuMI CCSGG 6 cut(s) 111, 127, 128, 143, 387, 481
BsaHI GRCGYC 2 cut(s) 234, 426
BsaI GGTCTC 4 cut(s) 119, 329, 608, 1139
BsaWI WCCGGW 3 cut(s) 173, 623, 974
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 392, 649, 1424
Bse1I ACTGG 4 cut(s) 356, 383, 654, 1210
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 5 cut(s) 62, 325, 344, 515, 1092
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 4 cut(s) 158, 392, 649, 1424
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 7 cut(s) 287, 864, 939, 1134, 1164, 1271, 1457
BseNI ACTGG 4 cut(s) 356, 383, 654, 1210
BsePI GCGCGC 1 cut(s) 367
BseRI GAGGAG 2 cut(s) 476, 626
BseSI GKGCMC 2 cut(s) 362, 386
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 4 cut(s) 496, 820, 823, 1330
BseYI CCCAGC 2 cut(s) 147, 1214
Bsh1236I CGCG 3 cut(s) 36, 303, 369
Bsh1285I CGRYCG 1 cut(s) 34
BshNI GGYRCC 2 cut(s) 233, 573
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 5 cut(s) 93, 254, 362, 468, 895
BsiHKCI CYCGRG 2 cut(s) 80, 126
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 4 cut(s) 158, 392, 649, 1424
BsmAI GTCTC 7 cut(s) 119, 329, 608, 949, 1076, 1139, 1187
BsmBI CGTCTC 1 cut(s) 1187
BsmFI GGGAC 1 cut(s) 43
Bso31I GGTCTC 4 cut(s) 119, 329, 608, 1139
BsoBI CYCGRG 2 cut(s) 80, 126
Bsp120I GGGCCC 1 cut(s) 382
Bsp1286I GDGCHC 7 cut(s) 93, 166, 254, 362, 386, 468, 895
Bsp143I GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
Bsp1720I GCTNAGC 1 cut(s) 1257
BspCNI CTCAG 7 cut(s) 286, 865, 940, 1135, 1165, 1270, 1456
BspFNI CGCG 3 cut(s) 36, 303, 369
BspHI TCATGA 1 cut(s) 1185
BspPI GGATC 5 cut(s) 171, 184, 433, 849, 862
BspQI GCTCTTC 3 cut(s) 900, 1277, 1353
BspT107I GGYRCC 2 cut(s) 233, 573
BspTNI GGTCTC 4 cut(s) 119, 329, 608, 1139
BsrBI CCGCTC 1 cut(s) 1287
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 4 cut(s) 356, 383, 654, 1210
BssHII GCGCGC 1 cut(s) 367
BssMI GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
BssNI GRCGYC 2 cut(s) 234, 426
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 1389
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 5 cut(s) 62, 325, 344, 515, 1092
Bst6I CTCTTC 4 cut(s) 438, 900, 1277, 1353
BstACI GRCGYC 2 cut(s) 234, 426
BstC8I GCNNGC 8 cut(s) 77, 93, 166, 301, 369, 651, 849, 1440
BstDEI CTNAG 7 cut(s) 273, 873, 948, 1143, 1173, 1257, 1443
BstF5I GGATG 1 cut(s) 192
BstFNI CGCG 3 cut(s) 36, 303, 369
BstH2I RGCGCY 2 cut(s) 237, 312
BstHHI GCGC 5 cut(s) 236, 311, 369, 371, 1104
BstKTI GATC 8 cut(s) 87, 179, 403, 408, 441, 857, 867, 1065
BstMAI GTCTC 7 cut(s) 119, 329, 608, 949, 1076, 1139, 1187
BstMBI GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 9 cut(s) 33, 128, 233, 290, 296, 320, 366, 899, 1381
BstNI CCWGG 5 cut(s) 62, 325, 344, 515, 1092
BstNSI RCATGY 2 cut(s) 58, 1072
BstPAI GACNNNNGTC 1 cut(s) 203
BstSLI GKGCMC 2 cut(s) 362, 386
BstUI CGCG 3 cut(s) 36, 303, 369
BstV1I GCAGC 4 cut(s) 496, 820, 823, 1330
BstV2I GAAGAC 6 cut(s) 310, 482, 492, 563, 908, 1129
BstX2I RGATCY 3 cut(s) 176, 400, 854
BstYI RGATCY 3 cut(s) 176, 400, 854
BstZI CGGCCG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 661, 765, 1203
Cac8I GCNNGC 8 cut(s) 77, 93, 166, 301, 369, 651, 849, 1440
CaiI CAGNNNCTG 1 cut(s) 1455
CciI TCATGA 1 cut(s) 1185
CfoI GCGC 5 cut(s) 236, 311, 369, 371, 1104
Cfr9I CCCGGG 1 cut(s) 126
CseI GACGC 1 cut(s) 434
Csp6I GTAC 5 cut(s) 40, 349, 574, 1447, 1453
CviAII CATG 6 cut(s) 55, 118, 187, 994, 1069, 1186
CviQI GTAC 5 cut(s) 40, 349, 574, 1447, 1453
DdeI CTNAG 7 cut(s) 273, 873, 948, 1143, 1173, 1257, 1443
DinI GGCGCC 1 cut(s) 235
DpnI GATC 8 cut(s) 86, 178, 402, 407, 440, 856, 866, 1064
DpnII GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
DrdI GACNNNNNNGTC 2 cut(s) 962, 1133
DseDI GACNNNNNNGTC 2 cut(s) 962, 1133
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 4 cut(s) 438, 900, 1277, 1353
EarI CTCTTC 4 cut(s) 438, 900, 1277, 1353
EciI GGCGGA 1 cut(s) 405
Ecl136II GAGCTC 2 cut(s) 91, 466
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 1389
Eco24I GRGCYC 4 cut(s) 93, 166, 386, 468
Eco31I GGTCTC 4 cut(s) 119, 329, 608, 1139
Eco47I GGWCC 4 cut(s) 241, 277, 1393, 1427
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 2 cut(s) 91, 466
Eco57I CTGAAG 2 cut(s) 881, 902
Eco88I CYCGRG 2 cut(s) 80, 126
EcoICRI GAGCTC 2 cut(s) 91, 466
EcoO109I RGGNCCY 2 cut(s) 382, 383
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 5 cut(s) 60, 323, 342, 513, 1090
EcoT14I CCWWGG 1 cut(s) 1389
EcoT38I GRGCYC 4 cut(s) 93, 166, 386, 468
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 1389
Esp3I CGTCTC 1 cut(s) 1187
FaeI CATG 6 cut(s) 58, 121, 190, 997, 1072, 1189
FaqI GGGAC 1 cut(s) 43
FatI CATG 6 cut(s) 54, 117, 186, 993, 1068, 1185
FauI CCCGC 2 cut(s) 173, 308
FblI GTMKAC 1 cut(s) 411
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 4 cut(s) 93, 166, 386, 468
GlaI GCGC 5 cut(s) 235, 310, 368, 370, 1103
GsaI CCCAGC 2 cut(s) 151, 1218
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 2 cut(s) 237, 312
HgaI GACGC 1 cut(s) 434
HhaI GCGC 5 cut(s) 236, 311, 369, 371, 1104
Hin1I GRCGYC 2 cut(s) 234, 426
Hin1II CATG 6 cut(s) 58, 121, 190, 997, 1072, 1189
Hin6I GCGC 5 cut(s) 234, 309, 367, 369, 1102
HinP1I GCGC 5 cut(s) 234, 309, 367, 369, 1102
HincII GTYRAC 2 cut(s) 412, 1000
HindII GTYRAC 2 cut(s) 412, 1000
HindIII AAGCTT 1 cut(s) 1361
HinfI GANTC 2 cut(s) 627, 692
HphI GGTGA 9 cut(s) 163, 434, 526, 824, 958, 1099, 1231, 1369, 1378
Hpy166II GTNNAC 3 cut(s) 360, 412, 1000
Hpy188I TCNGA 5 cut(s) 405, 438, 545, 610, 949
Hpy188III TCNNGA 8 cut(s) 16, 82, 196, 275, 374, 631, 868, 1186
Hpy8I GTNNAC 3 cut(s) 360, 412, 1000
Hpy99I CGWCG 4 cut(s) 17, 413, 416, 419
HpyAV CCTTC 5 cut(s) 163, 322, 580, 1297, 1330
HpyCH4IV ACGT 2 cut(s) 939, 1413
HpyCH4V TGCA 4 cut(s) 258, 360, 649, 1038
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 128, 233, 290, 296, 320, 366, 899, 1381
HpyF3I CTNAG 7 cut(s) 273, 873, 948, 1143, 1173, 1257, 1443
HpySE526I ACGT 2 cut(s) 939, 1413
Hsp92I GRCGYC 2 cut(s) 234, 426
Hsp92II CATG 6 cut(s) 58, 121, 190, 997, 1072, 1189
HspAI GCGC 5 cut(s) 234, 309, 367, 369, 1102
KasI GGCGCC 1 cut(s) 233
KpnI GGTACC 1 cut(s) 577
Kzo9I GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
LguI GCTCTTC 3 cut(s) 900, 1277, 1353
LmnI GCTCC 5 cut(s) 32, 161, 257, 463, 1367
Lsp1109I GCAGC 4 cut(s) 496, 820, 823, 1330
LweI GCATC 3 cut(s) 177, 636, 1113
MaeII ACGT 2 cut(s) 939, 1413
MaeIII GTNAC 5 cut(s) 422, 697, 734, 868, 964
MalI GATC 8 cut(s) 86, 178, 402, 407, 440, 856, 866, 1064
MbiI CCGCTC 1 cut(s) 1287
MboI GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
MfeI CAATTG 1 cut(s) 1419
MflI RGATCY 3 cut(s) 176, 400, 854
MhlI GDGCHC 7 cut(s) 93, 166, 254, 362, 386, 468, 895
MluCI AATT 2 cut(s) 134, 1419
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 2 cut(s) 636, 686
MmeI TCCRAC 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 790
MslI CAYNNNNRTG 3 cut(s) 447, 992, 1329
MspA1I CMGCKG 2 cut(s) 246, 526
MunI CAATTG 1 cut(s) 1419
MvaI CCWGG 5 cut(s) 62, 325, 344, 515, 1092
MvnI CGCG 3 cut(s) 36, 303, 369
MwoI GCNNNNNNNGC 9 cut(s) 33, 128, 233, 290, 296, 320, 366, 899, 1381
NarI GGCGCC 1 cut(s) 234
NciI CCSGG 6 cut(s) 111, 127, 128, 143, 387, 481
NdeII GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
NlaIII CATG 6 cut(s) 58, 121, 190, 997, 1072, 1189
NmeAIII GCCGAG 4 cut(s) 121, 210, 262, 484
NmuCI GTSAC 4 cut(s) 422, 734, 868, 964
NspI RCATGY 2 cut(s) 58, 1072
OliI CACNNNNGTG 1 cut(s) 447
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 1185
PauI GCGCGC 1 cut(s) 367
PciI ACATGT 1 cut(s) 54
PciSI GCTCTTC 3 cut(s) 900, 1277, 1353
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 93, 99
PdmI GAANNNNTTC 1 cut(s) 790
PflFI GACNNNGTC 2 cut(s) 941, 1150
PfoI TCCNGGA 1 cut(s) 342
PleI GAGTC 2 cut(s) 635, 686
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 2 cut(s) 635, 686
PscI ACATGT 1 cut(s) 54
PshAI GACNNNNGTC 1 cut(s) 203
Psp124BI GAGCTC 2 cut(s) 93, 468
Psp1406I AACGTT 1 cut(s) 1413
Psp6I CCWGG 5 cut(s) 60, 323, 342, 513, 1090
PspFI CCCAGC 2 cut(s) 147, 1214
PspGI CCWGG 5 cut(s) 60, 323, 342, 513, 1090
PspOMI GGGCCC 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 1455
PsuI RGATCY 3 cut(s) 176, 400, 854
PsyI GACNNNGTC 2 cut(s) 941, 1150
PteI GCGCGC 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 5 cut(s) 41, 350, 575, 1448, 1454
RsaNI GTAC 5 cut(s) 40, 349, 574, 1447, 1453
RseI CAYNNNNRTG 3 cut(s) 447, 992, 1329
SacI GAGCTC 2 cut(s) 93, 468
SalI GTCGAC 1 cut(s) 410
SapI GCTCTTC 3 cut(s) 900, 1277, 1353
Sau3AI GATC 8 cut(s) 84, 176, 400, 405, 438, 854, 864, 1062
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 2 cut(s) 636, 686
SduI GDGCHC 7 cut(s) 93, 166, 254, 362, 386, 468, 895
SfaNI GCATC 3 cut(s) 177, 636, 1113
SfiI GGCCNNNNNGGCC 1 cut(s) 128
SfoI GGCGCC 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 80
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 4 cut(s) 241, 277, 1393, 1427
SlaI CTCGAG 1 cut(s) 80
SmaI CCCGGG 1 cut(s) 128
SmiMI CAYNNNNRTG 3 cut(s) 447, 992, 1329
SmlI CTYRAG 5 cut(s) 80, 842, 932, 1079, 1262
SmoI CTYRAG 5 cut(s) 80, 842, 932, 1079, 1262
Sse9I AATT 2 cut(s) 134, 1419
SspDI GGCGCC 1 cut(s) 233
SstI GAGCTC 2 cut(s) 93, 468
StyI CCWWGG 1 cut(s) 1389
TaiI ACGT 2 cut(s) 942, 1416
TaqI TCGA 7 cut(s) 15, 81, 87, 102, 213, 411, 1065
TaqII GACCGA 1 cut(s) 575
TasI AATT 2 cut(s) 134, 1419
TatI WGTACW 3 cut(s) 39, 348, 1446
TauI GCSGC 6 cut(s) 36, 287, 290, 293, 314, 1290
TscAI CASTG 3 cut(s) 661, 772, 1210
TseFI GTSAC 4 cut(s) 422, 734, 868, 964
TseI GCWGC 4 cut(s) 484, 833, 836, 1343
Tsp45I GTSAC 4 cut(s) 422, 734, 868, 964
TspDTI ATGAA 4 cut(s) 599, 1031, 1202, 1340
TspMI CCCGGG 1 cut(s) 126
TspRI CASTG 3 cut(s) 661, 772, 1210
Tth111I GACNNNGTC 2 cut(s) 941, 1150
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 4 cut(s) 241, 277, 1393, 1427
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 58, 1072
XhoI CTCGAG 1 cut(s) 80
XmaI CCCGGG 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 790
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.