AT3G24130

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015806
Physical Location & Seq
Reverse (-)
8711663 .. 8713424
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24130.1

Sequence Viewer

Length: 1008 bp
ATGGGAACTCATCGAATTTTCATAGGTCTCATCGCTCTTTGTTGTTTTTGTCTACCTCACCTCATTGAAGCTAAGCCATTCGGAGTATACCAACAACAAGTATTTGTTGATCAATCCGGCCATGGAAATTTCACGACAATACAAAAAGCTATTGATTCTGTTCCAATCAACAATCGTCATTGGTTCTTCATCAACGTTAAAGCCGGTCTTTACAGAGAGAAAATAAAGATACCGTATGAAAAACCGTTCATAGTACTAGTAGGAGCTGGAAAAAGGTTAACGAGAGTTGAATGGGACGACCATTACTCGGTTGCACAAAGCCCTACCTTTTCTACTCTTGCCGATAACACCGTTGTTAAAAGCATCACTTTCGCCAATTCCTATAATTTCCCTAGCAAAGGGAAAATGAACAAGAACCCTAGAACTCCAGCCGTGGCAGCGTTGATAGGCGGCGATAAGTCGGCTTTTTACTCGGTAGGGTTTGCTGGAATTCAAGATACCTTGTGGGACTTTGATGGCCGGCACTACTTCCACCGGTGTACCATACAAGGCGCGGTCGATTTCATCTTTGGTACCGGTCAATCTATTTACCAGAGCTGTGTGATACAAGTGCTAGGTGGGCAGCTAGAACCGGGGCTAGCCGGTTACATAACGGCTCAAGGACGGACCAACCCGTACGATGCAAATGGATTCATCTTCATCAACTGCCTCGTATATGGAACAGGCATGGCCTTCTTGGGCAGGCCGTGGCGCGGTTATTCTCGAGTGATCTTTTACAACTCGAACCTGACCGATGTAGTTGTTCCTGAAGGATGGGATGCATGGAATTTCGTGGGCCACGAAAACCAGCTGGTCTTTGCAGAACATGGATGCTTTGGAAGTGGAGCAAATATAGGAAGACGTGTGAAGTGGGTTAAGAAGCTTAGTGAATCTGCCATTCAAAATTTGGCTGATCTCAGCTTTATTAATCGTGGTGGATGGGTTGAAGATTTGCCCATCCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

335

Amino Acids

37.27

Weight (kDa)

8.75

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 322 1.9e-61 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 1 cut(s) 572
AccI GTMKAC 2 cut(s) 52, 87
AccII CGCG 2 cut(s) 554, 753
AciI CCGC 3 cut(s) 450, 554, 753
AclI AACGTT 1 cut(s) 195
AcoI YGGCCR 2 cut(s) 118, 517
AcsI RAATTY 5 cut(s) 15, 127, 489, 826, 943
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 4 cut(s) 255, 541, 574, 677
AfiI CCNNNNNNNGG 3 cut(s) 307, 398, 752
AflIII ACRYGT 1 cut(s) 901
AgeI ACCGGT 2 cut(s) 534, 575
AgsI TTSAA 5 cut(s) 68, 290, 494, 941, 986
AhlI ACTAGT 1 cut(s) 256
AjiI CACGTC 1 cut(s) 902
AluBI AGCT 8 cut(s) 71, 149, 266, 597, 625, 850, 922, 960
AluI AGCT 8 cut(s) 71, 149, 266, 597, 625, 850, 922, 960
Alw26I GTCTC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 5 cut(s) 118, 517, 729, 743, 835
ApeKI GCWGC 2 cut(s) 437, 622
ApoI RAATTY 5 cut(s) 15, 127, 489, 826, 943
AseI ATTAAT 1 cut(s) 966
AsiGI ACCGGT 2 cut(s) 534, 575
Asp718I GGTACC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 554, 753
AspS9I GGNCC 2 cut(s) 666, 835
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 1 cut(s) 50
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 1 cut(s) 666
BaeI ACNNNNGTAYC 2 cut(s) 523, 556
BanI GGYRCC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 904
BbvI GCAGC 2 cut(s) 449, 634
BccI CCATC 4 cut(s) 509, 807, 972, 1004
BceAI ACGGC 3 cut(s) 416, 669, 730
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 32
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 6 cut(s) 257, 393, 420, 614, 626, 638
BisI GCNGC 3 cut(s) 438, 451, 623
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 3 cut(s) 439, 452, 624
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 633
Bme18I GGWCC 1 cut(s) 666
BmeT110I CYCGRG 1 cut(s) 762
BmgBI CACGTC 1 cut(s) 902
BmgT120I GGNCC 2 cut(s) 666, 835
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 1 cut(s) 633
BmsI GCATC 4 cut(s) 372, 670, 808, 860
BmtI GCTAGC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 904
BpmI CTGGAG 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 633
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 121, 432, 632, 746
BsaWI WCCGGW 2 cut(s) 534, 575
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 398, 752
Bse118I RCCGGY 5 cut(s) 203, 519, 534, 575, 641
BseDI CCNNGG 4 cut(s) 121, 432, 632, 746
BseGI GGATG 5 cut(s) 818, 823, 875, 983, 996
BseLI CCNNNNNNNGG 3 cut(s) 307, 398, 752
BseMII CTCAG 1 cut(s) 970
BseXI GCAGC 2 cut(s) 449, 634
Bsh1236I CGCG 2 cut(s) 554, 753
Bsh1285I CGRYCG 1 cut(s) 558
BshFI GGCC 5 cut(s) 120, 519, 731, 745, 837
BshNI GGYRCC 1 cut(s) 572
BshTI ACCGGT 2 cut(s) 534, 575
BsiEI CGRYCG 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 7 cut(s) 117, 204, 520, 535, 576, 632, 642
BsiWI CGTACG 1 cut(s) 675
BslFI GGGAC 2 cut(s) 308, 521
BslI CCNNNNNNNGG 3 cut(s) 307, 398, 752
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 308, 521
BsnI GGCC 5 cut(s) 120, 519, 731, 745, 837
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 762
Bsp143I GATC 3 cut(s) 109, 768, 952
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 3 cut(s) 450, 554, 753
BspANI GGCC 5 cut(s) 120, 519, 731, 745, 837
BspCNI CTCAG 1 cut(s) 969
BspFNI CGCG 2 cut(s) 554, 753
BspLI GGNNCC 1 cut(s) 574
BspOI GCTAGC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 32
BsrFI RCCGGY 5 cut(s) 203, 519, 534, 575, 641
BssAI RCCGGY 5 cut(s) 203, 519, 534, 575, 641
BssECI CCNNGG 4 cut(s) 121, 432, 632, 746
BssMI GATC 3 cut(s) 109, 768, 952
BssNAI GTATAC 1 cut(s) 88
BssT1I CCWWGG 1 cut(s) 121
Bst1107I GTATAC 1 cut(s) 88
Bst4CI ACNGT 3 cut(s) 234, 246, 352
BstC8I GCNNGC 3 cut(s) 521, 639, 743
BstDEI CTNAG 3 cut(s) 72, 923, 956
BstDSI CCRYGG 3 cut(s) 121, 432, 746
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 5 cut(s) 818, 823, 875, 983, 996
BstFNI CGCG 2 cut(s) 554, 753
BstHHI GCGC 2 cut(s) 554, 753
BstKTI GATC 3 cut(s) 112, 771, 955
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 109, 768, 952
BstMCI CGRYCG 1 cut(s) 558
BstMWI GCNNNNNNNGC 2 cut(s) 437, 619
BstSCI CCNGG 1 cut(s) 631
BstUI CGCG 2 cut(s) 554, 753
BstV1I GCAGC 2 cut(s) 449, 634
BstV2I GAAGAC 1 cut(s) 904
BstZ17I GTATAC 1 cut(s) 88
BsuRI GGCC 5 cut(s) 120, 519, 731, 745, 837
BtgI CCRYGG 3 cut(s) 121, 432, 746
BtgZI GCGATG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 902
BtsCI GGATG 5 cut(s) 818, 823, 875, 983, 996
Cac8I GCNNGC 3 cut(s) 521, 639, 743
CfoI GCGC 2 cut(s) 554, 753
Cfr10I RCCGGY 5 cut(s) 203, 519, 534, 575, 641
Cfr13I GGNCC 2 cut(s) 666, 835
Csp6I GTAC 4 cut(s) 254, 540, 573, 676
CspAI ACCGGT 2 cut(s) 534, 575
CviAII CATG 4 cut(s) 122, 727, 822, 866
CviQI GTAC 4 cut(s) 254, 540, 573, 676
DdeI CTNAG 3 cut(s) 72, 923, 956
DpnI GATC 3 cut(s) 111, 770, 954
DpnII GATC 3 cut(s) 109, 768, 952
EaeI YGGCCR 2 cut(s) 118, 517
Eco130I CCWWGG 1 cut(s) 121
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 828
Eco88I CYCGRG 1 cut(s) 762
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 4 cut(s) 125, 730, 825, 869
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 352, 384
FaqI GGGAC 2 cut(s) 308, 521
FatI CATG 4 cut(s) 121, 726, 821, 865
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 52, 87
Fnu4HI GCNGC 3 cut(s) 438, 451, 623
FokI GGATG 5 cut(s) 825, 830, 882, 983, 990
Fsp4HI GCNGC 3 cut(s) 438, 451, 623
FspBI CTAG 6 cut(s) 257, 393, 420, 614, 626, 638
GlaI GCGC 2 cut(s) 553, 752
GluI GCNGC 3 cut(s) 438, 451, 623
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 5 cut(s) 120, 519, 731, 745, 837
HapII CCGG 7 cut(s) 117, 204, 520, 535, 576, 632, 642
HhaI GCGC 2 cut(s) 554, 753
Hin1II CATG 4 cut(s) 125, 730, 825, 869
Hin6I GCGC 2 cut(s) 552, 751
HinP1I GCGC 2 cut(s) 552, 751
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 3 cut(s) 155, 690, 929
HpaI GTTAAC 1 cut(s) 279
HpaII CCGG 7 cut(s) 117, 204, 520, 535, 576, 632, 642
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 4 cut(s) 53, 88, 279, 540
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 4 cut(s) 133, 494, 762, 806
Hpy8I GTNNAC 4 cut(s) 53, 88, 279, 540
HpyAV CCTTC 2 cut(s) 742, 803
HpyCH4III ACNGT 3 cut(s) 234, 246, 352
HpyCH4IV ACGT 2 cut(s) 195, 901
HpyCH4V TGCA 4 cut(s) 314, 683, 821, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 619
HpyF3I CTNAG 3 cut(s) 72, 923, 956
HpySE526I ACGT 2 cut(s) 195, 901
Hsp92II CATG 4 cut(s) 125, 730, 825, 869
HspAI GCGC 2 cut(s) 552, 751
KpnI GGTACC 1 cut(s) 576
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
Ksp22I TGATCA 1 cut(s) 109
KspAI GTTAAC 1 cut(s) 279
Kzo9I GATC 3 cut(s) 109, 768, 952
LmnI GCTCC 2 cut(s) 263, 884
Lsp1109I GCAGC 2 cut(s) 449, 634
LweI GCATC 4 cut(s) 372, 670, 808, 860
MaeI CTAG 6 cut(s) 257, 393, 420, 614, 626, 638
MaeII ACGT 2 cut(s) 195, 901
MaeIII GTNAC 1 cut(s) 644
MalI GATC 3 cut(s) 111, 770, 954
MboI GATC 3 cut(s) 109, 768, 952
MboII GAAGA 4 cut(s) 178, 688, 909, 998
MluCI AATT 7 cut(s) 15, 127, 376, 385, 489, 826, 943
MnlI CCTC 3 cut(s) 66, 71, 719
Mph1103I ATGCAT 1 cut(s) 823
MroNI GCCGGC 1 cut(s) 519
MseI TTAA 5 cut(s) 198, 278, 357, 915, 966
MspA1I CMGCKG 1 cut(s) 850
MspI CCGG 7 cut(s) 117, 204, 520, 535, 576, 632, 642
MspR9I CCNGG 1 cut(s) 633
MvnI CGCG 2 cut(s) 554, 753
MwoI GCNNNNNNNGC 2 cut(s) 437, 619
NaeI GCCGGC 1 cut(s) 521
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 121
NdeII GATC 3 cut(s) 109, 768, 952
NgoMIV GCCGGC 1 cut(s) 519
NheI GCTAGC 1 cut(s) 637
NlaIII CATG 4 cut(s) 125, 730, 825, 869
NlaIV GGNNCC 1 cut(s) 574
NsiI ATGCAT 1 cut(s) 823
PaeR7I CTCGAG 1 cut(s) 762
PcsI WCGNNNNNNNCGW 1 cut(s) 837
PdiI GCCGGC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 155, 690, 929
Pfl23II CGTACG 1 cut(s) 675
PinAI ACCGGT 2 cut(s) 534, 575
PkrI GCNGC 3 cut(s) 439, 452, 624
PshBI ATTAAT 1 cut(s) 966
Psp1406I AACGTT 1 cut(s) 195
PspLI CGTACG 1 cut(s) 675
PspN4I GGNNCC 1 cut(s) 574
PspPI GGNCC 2 cut(s) 666, 835
PvuII CAGCTG 1 cut(s) 850
RsaI GTAC 4 cut(s) 255, 541, 574, 677
RsaNI GTAC 4 cut(s) 254, 540, 573, 676
SaqAI TTAA 5 cut(s) 198, 278, 357, 915, 966
SatI GCNGC 3 cut(s) 438, 451, 623
Sau3AI GATC 3 cut(s) 109, 768, 952
Sau96I GGNCC 2 cut(s) 666, 835
ScaI AGTACT 1 cut(s) 255
ScrFI CCNGG 1 cut(s) 633
SfaNI GCATC 4 cut(s) 372, 670, 808, 860
Sfr274I CTCGAG 1 cut(s) 762
SgrAI CRCCGGYG 1 cut(s) 534
SinI GGWCC 1 cut(s) 666
SlaI CTCGAG 1 cut(s) 762
SmlI CTYRAG 2 cut(s) 657, 762
SmoI CTYRAG 2 cut(s) 657, 762
SpeI ACTAGT 1 cut(s) 256
Sse9I AATT 7 cut(s) 15, 127, 376, 385, 489, 826, 943
SsiI CCGC 3 cut(s) 450, 554, 753
SspMI CTAG 6 cut(s) 257, 393, 420, 614, 626, 638
StyD4I CCNGG 1 cut(s) 631
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 3 cut(s) 234, 246, 352
TaiI ACGT 2 cut(s) 198, 904
TaqI TCGA 4 cut(s) 13, 558, 763, 782
TaqII GACCGA 1 cut(s) 806
TasI AATT 7 cut(s) 15, 127, 376, 385, 489, 826, 943
TatI WGTACW 1 cut(s) 253
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 3 cut(s) 155, 690, 929
Tru1I TTAA 5 cut(s) 198, 278, 357, 915, 966
Tru9I TTAA 5 cut(s) 198, 278, 357, 915, 966
TseI GCWGC 2 cut(s) 437, 622
TspDTI ATGAA 8 cut(s) 10, 178, 238, 252, 422, 553, 682, 688
TspGWI ACGGA 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 666
VspI ATTAAT 1 cut(s) 966
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 5 cut(s) 15, 127, 489, 826, 943
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 762
XmiI GTMKAC 2 cut(s) 52, 87
XspI CTAG 6 cut(s) 257, 393, 420, 614, 626, 638
ZrmI AGTACT 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.