MD04G1008100.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015806
Physical Location & Seq
Forward (+)
948800 .. 951491
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1008100.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGTCGTCGTTTTTCCAGTGTGTTGCCCTACTTTTAGTAGGTCTTGGATTATTTGGAGAAGCAGATGCCCAACTGTATAGAGTATACGGCAATAAGAAGATTGCTTATTACACCACCACCGTTGATAAATCCGGCCGTGGCGATTTCACCTCCATACAATCTGCCATCAATGCTGTTCCTAAAAACAACCGGCACTGGGTTTCTATCAAGATCAAGGCTGGCACGTATAGGGAAAAAGTGACTATCCCGAGTGATAAGCCGTACATAATTCTCAAAGGAGAAAATAGGCGCCAAACACAGATTGTTTGGGGTGATCATGACTCACTTGCACAAAGCCCTACTTTCGCCTCTTTCGCTGATAATATCATTGCCAGATCCATCACCTTTGTGAATTCATACAACAACCCTGTGAATAGCAACCCTCAAATGCCGGCAGTGGCGGCGATGGTAGCCGGTGACAAGTTGAAATTTTATCGATGTGGTTTCTTTGGGTTGCAAGACACTTTGTGGGATGTTAGTGGTCGTCACTACTATAAGCTTTGCACCATCCAGGGTGCGGTCGATTTCATCCTCGGCAGTGGCCAATCCATTTTTGAGAAATGTTCCATATCAGTTCTTGGTGGAGCTTTGGGACGTGGTGTGGCGGGTTTTATCACAGCACAGGGAAGAGACAACCCAAAGGATCCCAATGGGTTTGTGTTCAAGGATTGCAAAGTGACTGGTACTGGTTCAACCTTCTTGGGAAGGCCATGGAGAGGTTACTCAAGGGTCATATTCTACAATTCCAATTTCTCAAACGTCGTCGTTCCTGAGGGCTGGGCTCCTTGGAATATGGTTGGCCACGAAGAGCAATTGACGTACGCAGAGCAGGAGTGCTATGGACCAGGAGCCAACACTTCAAAGCGAGTGAGTTGGGAAAAGAAGCTTAGCAGTGATACAGTGCAGCAGTTTGCAAGCTTGGAATTTATTAATGCTGATGGATGGCTCAGTGACCAGCCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

335

Amino Acids

36.99

Weight (kDa)

8.89

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 40 - 325 4.4e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 84
AciI CCGC 3 cut(s) 440, 557, 644
AclWI GGATC 3 cut(s) 369, 677, 690
AcoI YGGCCR 3 cut(s) 133, 580, 838
AcsI RAATTY 3 cut(s) 391, 467, 962
AcyI GRCGYC 1 cut(s) 289
AdeI CACNNNGTG 1 cut(s) 507
AfaI GTAC 3 cut(s) 263, 724, 860
AfiI CCNNNNNNNGG 3 cut(s) 196, 556, 755
AgsI TTSAA 4 cut(s) 466, 703, 732, 900
AjiI CACGTC 1 cut(s) 635
AjnI CCWGG 2 cut(s) 549, 883
AleI CACNNNNGTG 1 cut(s) 386
AluBI AGCT 4 cut(s) 538, 626, 925, 957
AluI AGCT 4 cut(s) 538, 626, 925, 957
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 3 cut(s) 369, 677, 690
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 4 cut(s) 133, 580, 746, 838
ApeKI GCWGC 1 cut(s) 943
ApoI RAATTY 3 cut(s) 391, 467, 962
AseI ATTAAT 1 cut(s) 969
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 881
AsuHPI GGTGA 4 cut(s) 139, 323, 373, 467
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 1 cut(s) 881
AxyI CCTNAGG 1 cut(s) 810
BalI TGGCCA 2 cut(s) 582, 840
BamHI GGATCC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 823
BbvI GCAGC 1 cut(s) 955
BccI CCATC 6 cut(s) 173, 386, 439, 554, 971, 975
BceAI ACGGC 3 cut(s) 103, 120, 244
BciT130I CCWGG 2 cut(s) 551, 885
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 292
BisI GCNGC 2 cut(s) 441, 944
BlpI GCTNAGC 1 cut(s) 926
BlsI GCNGC 2 cut(s) 442, 945
Bme1390I CCNGG 2 cut(s) 551, 885
Bme18I GGWCC 1 cut(s) 881
BmeT110I CYCGRG 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 881
BmiI GGNNCC 4 cut(s) 290, 684, 822, 889
BmrFI CCNGG 2 cut(s) 551, 885
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 1 cut(s) 55
BmuI ACTGGG 1 cut(s) 205
Bpu1102I GCTNAGC 1 cut(s) 926
BpuEI CTTGAG 1 cut(s) 748
Bsa29I ATCGAT 1 cut(s) 475
BsaAI YACGTR 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 289
BsaJI CCNNGG 5 cut(s) 136, 550, 571, 749, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 556, 755
Bse118I RCCGGY 3 cut(s) 189, 430, 452
Bse1I ACTGG 4 cut(s) 16, 200, 724, 730
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 2 cut(s) 551, 885
BseCI ATCGAT 1 cut(s) 475
BseDI CCNNGG 5 cut(s) 136, 550, 571, 749, 824
BseGI GGATG 4 cut(s) 517, 546, 567, 986
BseLI CCNNNNNNNGG 3 cut(s) 196, 556, 755
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 801, 1000
BseNI ACTGG 4 cut(s) 16, 200, 724, 730
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 955
BseYI CCCAGC 1 cut(s) 816
BsgI GTGCAG 1 cut(s) 962
Bsh1285I CGRYCG 2 cut(s) 136, 561
BshFI GGCC 4 cut(s) 135, 582, 748, 840
BshNI GGYRCC 1 cut(s) 288
BshVI ATCGAT 1 cut(s) 475
BsiEI CGRYCG 2 cut(s) 136, 561
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 4 cut(s) 132, 190, 431, 453
BsiWI CGTACG 1 cut(s) 858
BslFI GGGAC 1 cut(s) 645
BslI CCNNNNNNNGG 3 cut(s) 196, 556, 755
BsmAI GTCTC 1 cut(s) 663
BsmFI GGGAC 1 cut(s) 645
BsnI GGCC 4 cut(s) 135, 582, 748, 840
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 823
Bsp143I GATC 4 cut(s) 210, 313, 374, 682
Bsp1720I GCTNAGC 1 cut(s) 926
Bsp19I CCATGG 1 cut(s) 749
BspACI CCGC 3 cut(s) 440, 557, 644
BspANI GGCC 4 cut(s) 135, 582, 748, 840
BspCNI CTCAG 2 cut(s) 802, 999
BspDI ATCGAT 1 cut(s) 475
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 4 cut(s) 290, 684, 822, 889
BspPI GGATC 3 cut(s) 369, 677, 690
BspQI GCTCTTC 1 cut(s) 840
BspT107I GGYRCC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 3 cut(s) 189, 430, 452
BsrI ACTGG 4 cut(s) 16, 200, 724, 730
BssAI RCCGGY 3 cut(s) 189, 430, 452
BssECI CCNNGG 5 cut(s) 136, 550, 571, 749, 824
BssMI GATC 4 cut(s) 210, 313, 374, 682
BssNAI GTATAC 1 cut(s) 85
BssNI GRCGYC 1 cut(s) 289
BssT1I CCWWGG 2 cut(s) 749, 824
Bst1107I GTATAC 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 551, 885
Bst4CI ACNGT 3 cut(s) 75, 121, 940
Bst6I CTCTTC 2 cut(s) 661, 840
BstACI GRCGYC 1 cut(s) 289
BstBAI YACGTR 1 cut(s) 225
BstC8I GCNNGC 3 cut(s) 220, 432, 955
BstDEI CTNAG 3 cut(s) 810, 926, 986
BstDSI CCRYGG 2 cut(s) 136, 749
BstF5I GGATG 4 cut(s) 517, 546, 567, 986
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 4 cut(s) 213, 316, 377, 685
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 4 cut(s) 210, 313, 374, 682
BstMCI CGRYCG 2 cut(s) 136, 561
BstMWI GCNNNNNNNGC 4 cut(s) 170, 353, 440, 449
BstNI CCWGG 2 cut(s) 551, 885
BstSCI CCNGG 2 cut(s) 549, 883
BstV1I GCAGC 1 cut(s) 955
BstX2I RGATCY 2 cut(s) 374, 682
BstYI RGATCY 2 cut(s) 374, 682
BstZ17I GTATAC 1 cut(s) 85
BstZI CGGCCG 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 4 cut(s) 135, 582, 748, 840
BsuTUI ATCGAT 1 cut(s) 475
BtgI CCRYGG 2 cut(s) 136, 749
BtgZI GCGATG 1 cut(s) 458
BtrI CACGTC 1 cut(s) 635
BtsCI GGATG 4 cut(s) 517, 546, 567, 986
BtsI GCAGTG 3 cut(s) 441, 583, 937
BtsIMutI CAGTG 7 cut(s) 23, 193, 441, 583, 937, 945, 994
Cac8I GCNNGC 3 cut(s) 220, 432, 955
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 3 cut(s) 189, 430, 452
Cfr13I GGNCC 1 cut(s) 881
ClaI ATCGAT 1 cut(s) 475
Csp6I GTAC 3 cut(s) 262, 723, 859
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 2 cut(s) 317, 750
CviQI GTAC 3 cut(s) 262, 723, 859
DdeI CTNAG 3 cut(s) 810, 926, 986
DinI GGCGCC 1 cut(s) 290
DpnI GATC 4 cut(s) 212, 315, 376, 684
DpnII GATC 4 cut(s) 210, 313, 374, 682
DraIII CACNNNGTG 1 cut(s) 507
EaeI YGGCCR 3 cut(s) 133, 580, 838
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 661, 840
EarI CTCTTC 2 cut(s) 661, 840
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 749, 824
Eco24I GRGCYC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 881
Eco52I CGGCCG 1 cut(s) 133
Eco81I CCTNAGG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 549, 883
EcoT14I CCWWGG 2 cut(s) 749, 824
EcoT38I GRGCYC 1 cut(s) 823
EgeI GGCGCC 1 cut(s) 290
EheI GGCGCC 1 cut(s) 290
ErhI CCWWGG 2 cut(s) 749, 824
FaeI CATG 2 cut(s) 320, 753
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FaqI GGGAC 1 cut(s) 645
FatI CATG 2 cut(s) 316, 749
FauI CCCGC 1 cut(s) 637
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 441, 944
FokI GGATG 4 cut(s) 524, 533, 554, 993
FriOI GRGCYC 1 cut(s) 823
Fsp4HI GCNGC 2 cut(s) 441, 944
GlaI GCGC 1 cut(s) 290
GluI GCNGC 2 cut(s) 441, 944
GsaI CCCAGC 1 cut(s) 820
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 4 cut(s) 135, 582, 748, 840
HapII CCGG 4 cut(s) 132, 190, 431, 453
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 289
Hin1II CATG 2 cut(s) 320, 753
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HindIII AAGCTT 3 cut(s) 536, 923, 955
HinfI GANTC 1 cut(s) 320
HpaII CCGG 4 cut(s) 132, 190, 431, 453
HphI GGTGA 4 cut(s) 139, 323, 373, 467
Hpy166II GTNNAC 1 cut(s) 85
Hpy188III TCNNGA 4 cut(s) 208, 247, 317, 809
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 3 cut(s) 10, 803, 806
HpyAV CCTTC 2 cut(s) 738, 745
HpyCH4III ACNGT 3 cut(s) 75, 121, 940
HpyCH4IV ACGT 4 cut(s) 224, 634, 798, 857
HpyCH4V TGCA 6 cut(s) 329, 496, 543, 711, 943, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 353, 440, 449
HpyF3I CTNAG 3 cut(s) 810, 926, 986
HpySE526I ACGT 4 cut(s) 224, 634, 798, 857
Hsp92I GRCGYC 1 cut(s) 289
Hsp92II CATG 2 cut(s) 320, 753
HspAI GCGC 1 cut(s) 289
KasI GGCGCC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 430
KroNI GCCGGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 4 cut(s) 210, 313, 374, 682
LguI GCTCTTC 1 cut(s) 840
LmnI GCTCC 3 cut(s) 623, 826, 887
Lsp1109I GCAGC 1 cut(s) 955
LweI GCATC 1 cut(s) 55
MaeII ACGT 4 cut(s) 224, 634, 798, 857
MaeIII GTNAC 6 cut(s) 238, 455, 524, 715, 758, 989
MalI GATC 4 cut(s) 212, 315, 376, 684
MboI GATC 4 cut(s) 210, 313, 374, 682
MboII GAAGA 3 cut(s) 109, 678, 857
MfeI CAATTG 1 cut(s) 851
MflI RGATCY 2 cut(s) 374, 682
MhlI GDGCHC 1 cut(s) 823
MlsI TGGCCA 2 cut(s) 582, 840
MluCI AATT 7 cut(s) 267, 391, 467, 781, 787, 851, 962
MluNI TGGCCA 2 cut(s) 582, 840
Mly113I GGCGCC 1 cut(s) 289
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 6 cut(s) 160, 358, 432, 581, 749, 805
Mox20I TGGCCA 2 cut(s) 582, 840
MroNI GCCGGC 1 cut(s) 430
MscI TGGCCA 2 cut(s) 582, 840
MseI TTAA 1 cut(s) 969
MslI CAYNNNNRTG 1 cut(s) 386
Msp20I TGGCCA 2 cut(s) 582, 840
MspI CCGG 4 cut(s) 132, 190, 431, 453
MspR9I CCNGG 2 cut(s) 551, 885
MunI CAATTG 1 cut(s) 851
MvaI CCWGG 2 cut(s) 551, 885
MwoI GCNNNNNNNGC 4 cut(s) 170, 353, 440, 449
NaeI GCCGGC 1 cut(s) 432
NarI GGCGCC 1 cut(s) 289
NcoI CCATGG 1 cut(s) 749
NdeII GATC 4 cut(s) 210, 313, 374, 682
NgoMIV GCCGGC 1 cut(s) 430
NlaIII CATG 2 cut(s) 320, 753
NlaIV GGNNCC 4 cut(s) 290, 684, 822, 889
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 5 cut(s) 238, 455, 524, 715, 989
OliI CACNNNNGTG 1 cut(s) 386
PagI TCATGA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 840
PdiI GCCGGC 1 cut(s) 432
Pfl23II CGTACG 1 cut(s) 858
PkrI GCNGC 2 cut(s) 442, 945
PleI GAGTC 1 cut(s) 314
PluTI GGCGCC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 225
PshBI ATTAAT 1 cut(s) 969
Psp6I CCWGG 2 cut(s) 549, 883
PspFI CCCAGC 1 cut(s) 816
PspGI CCWGG 2 cut(s) 549, 883
PspLI CGTACG 1 cut(s) 858
PspN4I GGNNCC 4 cut(s) 290, 684, 822, 889
PspPI GGNCC 1 cut(s) 881
PsuI RGATCY 2 cut(s) 374, 682
RsaI GTAC 3 cut(s) 263, 724, 860
RsaNI GTAC 3 cut(s) 262, 723, 859
RseI CAYNNNNRTG 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 969
SatI GCNGC 2 cut(s) 441, 944
Sau3AI GATC 4 cut(s) 210, 313, 374, 682
Sau96I GGNCC 1 cut(s) 881
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 551, 885
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 1 cut(s) 55
SfoI GGCGCC 1 cut(s) 290
SinI GGWCC 1 cut(s) 881
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 763
SmoI CTYRAG 1 cut(s) 763
Sse9I AATT 7 cut(s) 267, 391, 467, 781, 787, 851, 962
SsiI CCGC 3 cut(s) 440, 557, 644
SspDI GGCGCC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 549, 883
StyI CCWWGG 2 cut(s) 749, 824
TaaI ACNGT 3 cut(s) 75, 121, 940
TaiI ACGT 4 cut(s) 227, 637, 801, 860
TaqI TCGA 2 cut(s) 475, 561
TasI AATT 7 cut(s) 267, 391, 467, 781, 787, 851, 962
TauI GCSGC 1 cut(s) 443
Tru1I TTAA 1 cut(s) 969
Tru9I TTAA 1 cut(s) 969
TscAI CASTG 7 cut(s) 23, 200, 441, 583, 937, 945, 994
TseFI GTSAC 5 cut(s) 238, 455, 524, 715, 989
TseI GCWGC 1 cut(s) 943
Tsp45I GTSAC 5 cut(s) 238, 455, 524, 715, 989
TspDTI ATGAA 2 cut(s) 384, 556
TspRI CASTG 7 cut(s) 23, 200, 441, 583, 937, 945, 994
VpaK11BI GGWCC 1 cut(s) 881
VspI ATTAAT 1 cut(s) 969
XapI RAATTY 3 cut(s) 391, 467, 962
XmiI GTMKAC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.