AT5G18990

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015806
Physical Location & Seq
Forward (+)
6340076 .. 6341699
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18990.1

Sequence Viewer

Length: 993 bp
ATGGGAACTCATCGAATTATCCTTGGTCTCGCCGCTCTTTGTTGTTTTTGTCTACCTCATCTCATTGAAGCTAAACCATTCGAAGTGATCGTCGATCAATCGGGCCATGGAAATTTCACGACCATACAAAAAGCTATTGATTCGGTCCCAATCAACAATACTCATTGGTTCTTCATCAACGTTAAAGCCGGCCTTTACAGAGAGAAAATAACGATACCGCAAAAAAAACCTTTCATAGTAATCGTGGGAGCTGGGAAACGGTCAACGAGAGTAGAATGGGACGACCATGCCTCGCTTGCACAAAGCCCTACCTTTGCTACTCTAGCTGATAACACCGTCGTTAAAAAGATCACTTTCGCCAATTCCTACAACTTCCCTAGCAATGGGAAAATAAACAAGAACCCTAGAGTTCCGGCCGTGGCAGCATTTATCGGCGGTGATAAGTCGGCTTTTTACTCGGTAGGATTTGCTGGAATTCAAGATACCTTGTGGGACTCGGATGGCCGACACTACTTCCATAGATGCACCATCCAAGGCGCGGTTGATTTCATCTTAGGGAGCGGCCAATCTATTTACCAGAGCTGTGTGATACAAGTGCTAGGTGGGCAGCTAGGACCGGGGGTAACGGGTTACATAACGGCTCAAGGACGGACCAACGCAAACGACGCGAATGGATTCGTATTCATCAACTGCCTCGTTCATGGATTCGGCAAGGCTTACTTGGGCAGAGCCTGGCGCCCATACTCTCGAGTGATATTTTACAACTCGAACCTGACGGATGTGGTTGATCCTCTAGGATGGTGGGAATGGAACTACCAGGGTTACGAAAAGCAGCTGACCTATGCAGAACATGGATGCTTCGGAAGTGGATCAAATACATCAAGACGTGCCAAATGGGTTAAGAAGCTGAGTGCATCTGCCGTCCAACATTTGGCTGATCTCAGCTTCATTAATCGTGGTGGATGGGTTGAAGATTTACCCATCCGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

330

Amino Acids

36.46

Weight (kDa)

9.18

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 29 - 307 1e-62 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 735
AccB7I CCANNNNNTGG 1 cut(s) 931
AccBSI CCGCTC 2 cut(s) 35, 561
AccI GTMKAC 1 cut(s) 52
AccII CGCG 2 cut(s) 539, 668
AciI CCGC 5 cut(s) 33, 218, 435, 539, 561
AclI AACGTT 1 cut(s) 180
AclWI GGATC 2 cut(s) 782, 877
AcoI YGGCCR 3 cut(s) 414, 502, 562
AcsI RAATTY 2 cut(s) 112, 474
AcyI GRCGYC 1 cut(s) 736
AfiI CCNNNNNNNGG 3 cut(s) 383, 538, 931
AgsI TTSAA 3 cut(s) 68, 479, 971
AjiI CACGTC 1 cut(s) 887
AjnI CCWGG 2 cut(s) 731, 816
AluBI AGCT 9 cut(s) 71, 134, 251, 326, 582, 610, 835, 907, 945
AluI AGCT 9 cut(s) 71, 134, 251, 326, 582, 610, 835, 907, 945
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 782, 877
AlwNI CAGNNNCTG 1 cut(s) 732
Ama87I CYCGRG 1 cut(s) 747
AoxI GGCC 5 cut(s) 103, 190, 414, 502, 562
ApeKI GCWGC 3 cut(s) 422, 607, 832
ApoI RAATTY 2 cut(s) 112, 474
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AseI ATTAAT 1 cut(s) 951
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 2 cut(s) 539, 738
AspS9I GGNCC 4 cut(s) 103, 145, 614, 651
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 449
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 747
AvaII GGWCC 3 cut(s) 145, 614, 651
BanI GGYRCC 1 cut(s) 735
BbvI GCAGC 3 cut(s) 434, 619, 844
BccI CCATC 5 cut(s) 494, 536, 792, 957, 989
BceAI ACGGC 3 cut(s) 401, 654, 905
BciT130I CCWGG 2 cut(s) 733, 818
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 6 cut(s) 323, 378, 405, 599, 611, 794
BfoI RGCGCY 1 cut(s) 739
BisI GCNGC 5 cut(s) 33, 423, 562, 608, 833
BlsI GCNGC 5 cut(s) 34, 424, 563, 609, 834
Bme1390I CCNGG 3 cut(s) 618, 733, 818
Bme18I GGWCC 3 cut(s) 145, 614, 651
BmeT110I CYCGRG 1 cut(s) 747
BmgBI CACGTC 1 cut(s) 887
BmgT120I GGNCC 4 cut(s) 103, 145, 614, 651
BmiI GGNNCC 2 cut(s) 147, 737
BmrFI CCNGG 3 cut(s) 618, 733, 818
BmsI GCATC 3 cut(s) 512, 845, 923
Bpu14I TTCGAA 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 627
BpuMI CCSGG 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 6 cut(s) 22, 106, 417, 532, 617, 817
Bsc4I CCNNNNNNNGG 3 cut(s) 383, 538, 931
Bse118I RCCGGY 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 2 cut(s) 733, 818
BseDI CCNNGG 6 cut(s) 22, 106, 417, 532, 617, 817
BseGI GGATG 7 cut(s) 505, 528, 784, 803, 860, 968, 981
BseLI CCNNNNNNNGG 3 cut(s) 383, 538, 931
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 899, 955
BseX3I CGGCCG 1 cut(s) 414
BseXI GCAGC 3 cut(s) 434, 619, 844
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 2 cut(s) 539, 668
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 5 cut(s) 105, 192, 416, 504, 564
BshNI GGYRCC 1 cut(s) 735
BsiEI CGRYCG 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 747
BsiSI CCGG 3 cut(s) 189, 413, 617
BslFI GGGAC 3 cut(s) 131, 293, 506
BslI CCNNNNNNNGG 3 cut(s) 383, 538, 931
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 3 cut(s) 131, 293, 506
BsnI GGCC 5 cut(s) 105, 192, 416, 504, 564
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 747
Bsp119I TTCGAA 1 cut(s) 81
Bsp143I GATC 6 cut(s) 87, 94, 348, 787, 869, 937
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 5 cut(s) 33, 218, 435, 539, 561
BspANI GGCC 5 cut(s) 105, 192, 416, 504, 564
BspCNI CTCAG 2 cut(s) 900, 954
BspFNI CGCG 2 cut(s) 539, 668
BspLI GGNNCC 2 cut(s) 147, 737
BspPI GGATC 2 cut(s) 782, 877
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 32
BsrBI CCGCTC 2 cut(s) 35, 561
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 6 cut(s) 22, 106, 417, 532, 617, 817
BssMI GATC 6 cut(s) 87, 94, 348, 787, 869, 937
BssNI GRCGYC 1 cut(s) 736
BssT1I CCWWGG 3 cut(s) 22, 106, 532
Bst2UI CCWGG 2 cut(s) 733, 818
Bst4CI ACNGT 2 cut(s) 261, 337
BstACI GRCGYC 1 cut(s) 736
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 190, 297
BstDEI CTNAG 3 cut(s) 553, 908, 941
BstDSI CCRYGG 2 cut(s) 106, 417
BstF5I GGATG 7 cut(s) 505, 528, 784, 803, 860, 968, 981
BstFNI CGCG 2 cut(s) 539, 668
BstH2I RGCGCY 1 cut(s) 739
BstHHI GCGC 2 cut(s) 539, 738
BstKTI GATC 6 cut(s) 90, 97, 351, 790, 872, 940
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 6 cut(s) 87, 94, 348, 787, 869, 937
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 5 cut(s) 296, 323, 422, 604, 665
BstNI CCWGG 2 cut(s) 733, 818
BstSCI CCNGG 3 cut(s) 616, 731, 816
BstUI CGCG 2 cut(s) 539, 668
BstV1I GCAGC 3 cut(s) 434, 619, 844
BstZI CGGCCG 1 cut(s) 414
BsuRI GGCC 5 cut(s) 105, 192, 416, 504, 564
BtgI CCRYGG 2 cut(s) 106, 417
BtrI CACGTC 1 cut(s) 887
BtsCI GGATG 7 cut(s) 505, 528, 784, 803, 860, 968, 981
Cac8I GCNNGC 2 cut(s) 190, 297
CaiI CAGNNNCTG 1 cut(s) 732
CfoI GCGC 2 cut(s) 539, 738
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 4 cut(s) 103, 145, 614, 651
CseI GACGC 1 cut(s) 674
CviAII CATG 4 cut(s) 107, 287, 701, 851
DdeI CTNAG 3 cut(s) 553, 908, 941
DinI GGCGCC 1 cut(s) 737
DpnI GATC 6 cut(s) 89, 96, 350, 789, 871, 939
DpnII GATC 6 cut(s) 87, 94, 348, 787, 869, 937
EaeI YGGCCR 3 cut(s) 414, 502, 562
EagI CGGCCG 1 cut(s) 414
EclXI CGGCCG 1 cut(s) 414
Eco130I CCWWGG 3 cut(s) 22, 106, 532
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 3 cut(s) 145, 614, 651
Eco52I CGGCCG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 731, 816
EcoT14I CCWWGG 3 cut(s) 22, 106, 532
EgeI GGCGCC 1 cut(s) 737
EheI GGCGCC 1 cut(s) 737
ErhI CCWWGG 3 cut(s) 22, 106, 532
FaeI CATG 4 cut(s) 110, 290, 704, 854
FalI AAGNNNNNCTT 4 cut(s) 177, 209, 704, 736
FaqI GGGAC 3 cut(s) 131, 293, 506
FatI CATG 4 cut(s) 106, 286, 700, 850
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 33, 423, 562, 608, 833
FokI GGATG 7 cut(s) 512, 515, 791, 810, 867, 968, 975
Fsp4HI GCNGC 5 cut(s) 33, 423, 562, 608, 833
FspBI CTAG 6 cut(s) 323, 378, 405, 599, 611, 794
GlaI GCGC 2 cut(s) 538, 737
GluI GCNGC 5 cut(s) 33, 423, 562, 608, 833
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 739
HaeIII GGCC 5 cut(s) 105, 192, 416, 504, 564
HapII CCGG 3 cut(s) 189, 413, 617
HgaI GACGC 1 cut(s) 674
HhaI GCGC 2 cut(s) 539, 738
Hin1I GRCGYC 1 cut(s) 736
Hin1II CATG 4 cut(s) 110, 290, 704, 854
Hin6I GCGC 2 cut(s) 537, 736
HinP1I GCGC 2 cut(s) 537, 736
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 4 cut(s) 140, 494, 675, 705
HpaII CCGG 3 cut(s) 189, 413, 617
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 53, 264
Hpy188I TCNGA 2 cut(s) 499, 863
Hpy188III TCNNGA 4 cut(s) 118, 479, 747, 882
Hpy8I GTNNAC 2 cut(s) 53, 264
Hpy99I CGWCG 3 cut(s) 95, 341, 668
HpyCH4III ACNGT 2 cut(s) 261, 337
HpyCH4IV ACGT 2 cut(s) 180, 886
HpyCH4V TGCA 4 cut(s) 299, 525, 845, 914
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 323, 422, 604, 665
HpyF3I CTNAG 3 cut(s) 553, 908, 941
HpySE526I ACGT 2 cut(s) 180, 886
Hsp92I GRCGYC 1 cut(s) 736
Hsp92II CATG 4 cut(s) 110, 290, 704, 854
HspAI GCGC 2 cut(s) 537, 736
KasI GGCGCC 1 cut(s) 735
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 6 cut(s) 87, 94, 348, 787, 869, 937
LmnI GCTCC 2 cut(s) 248, 558
Lsp1109I GCAGC 3 cut(s) 434, 619, 844
LweI GCATC 3 cut(s) 512, 845, 923
MaeI CTAG 6 cut(s) 323, 378, 405, 599, 611, 794
MaeII ACGT 2 cut(s) 180, 886
MaeIII GTNAC 3 cut(s) 622, 629, 821
MalI GATC 6 cut(s) 89, 96, 350, 789, 871, 939
MbiI CCGCTC 2 cut(s) 35, 561
MboI GATC 6 cut(s) 87, 94, 348, 787, 869, 937
MboII GAAGA 2 cut(s) 163, 983
MluCI AATT 4 cut(s) 15, 112, 361, 474
Mly113I GGCGCC 1 cut(s) 736
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 949
MnlI CCTC 4 cut(s) 66, 301, 704, 801
MroNI GCCGGC 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 4 cut(s) 183, 342, 900, 951
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 3 cut(s) 189, 413, 617
MspR9I CCNGG 3 cut(s) 618, 733, 818
MvaI CCWGG 2 cut(s) 733, 818
MvnI CGCG 2 cut(s) 539, 668
MwoI GCNNNNNNNGC 5 cut(s) 296, 323, 422, 604, 665
NaeI GCCGGC 1 cut(s) 190
NarI GGCGCC 1 cut(s) 736
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 1 cut(s) 106
NdeII GATC 6 cut(s) 87, 94, 348, 787, 869, 937
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 4 cut(s) 110, 290, 704, 854
NlaIV GGNNCC 2 cut(s) 147, 737
NspV TTCGAA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 3 cut(s) 140, 675, 705
PflMI CCANNNNNTGG 1 cut(s) 931
PkrI GCNGC 5 cut(s) 34, 424, 563, 609, 834
PleI GAGTC 1 cut(s) 488
PluTI GGCGCC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 488
PshBI ATTAAT 1 cut(s) 951
Psp1406I AACGTT 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 731, 816
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 2 cut(s) 731, 816
PspN4I GGNNCC 2 cut(s) 147, 737
PspPI GGNCC 4 cut(s) 103, 145, 614, 651
PstNI CAGNNNCTG 1 cut(s) 732
PvuII CAGCTG 1 cut(s) 835
SaqAI TTAA 4 cut(s) 183, 342, 900, 951
SatI GCNGC 5 cut(s) 33, 423, 562, 608, 833
Sau3AI GATC 6 cut(s) 87, 94, 348, 787, 869, 937
Sau96I GGNCC 4 cut(s) 103, 145, 614, 651
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 3 cut(s) 618, 733, 818
SfaNI GCATC 3 cut(s) 512, 845, 923
SfoI GGCGCC 1 cut(s) 737
Sfr274I CTCGAG 1 cut(s) 747
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 3 cut(s) 145, 614, 651
SlaI CTCGAG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 642, 747
SmoI CTYRAG 2 cut(s) 642, 747
Sse9I AATT 4 cut(s) 15, 112, 361, 474
SsiI CCGC 5 cut(s) 33, 218, 435, 539, 561
SspDI GGCGCC 1 cut(s) 735
SspMI CTAG 6 cut(s) 323, 378, 405, 599, 611, 794
StyD4I CCNGG 3 cut(s) 616, 731, 816
StyI CCWWGG 3 cut(s) 22, 106, 532
TaaI ACNGT 2 cut(s) 261, 337
TaiI ACGT 2 cut(s) 183, 889
TaqI TCGA 5 cut(s) 13, 81, 93, 748, 767
TaqII GACCGA 1 cut(s) 133
TasI AATT 4 cut(s) 15, 112, 361, 474
TauI GCSGC 2 cut(s) 35, 564
TfiI GAWTC 3 cut(s) 140, 675, 705
Tru1I TTAA 4 cut(s) 183, 342, 900, 951
Tru9I TTAA 4 cut(s) 183, 342, 900, 951
TseI GCWGC 3 cut(s) 422, 607, 832
TspDTI ATGAA 6 cut(s) 163, 223, 538, 673, 689, 937
TspGWI ACGGA 3 cut(s) 664, 791, 974
Van91I CCANNNNNTGG 1 cut(s) 931
VpaK11BI GGWCC 3 cut(s) 145, 614, 651
VspI ATTAAT 1 cut(s) 951
XapI RAATTY 2 cut(s) 112, 474
XhoI CTCGAG 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 6 cut(s) 323, 378, 405, 599, 611, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.