FvH4_4g15880

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015806
Physical Location & Seq
Forward (+)
19811255 .. 19813457
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15880.t1

Sequence Viewer

Length: 1026 bp
ATGACAATATTTCCTCCATGGTTTGGTTACTATTCATTTGTCGTCATCTTGCTAGGGTTTGGATTAGGGCAACTAGGTACTCATGGCCAGATCTATAGATTATACGGCCAAAAGAAATTAGCTACTTACACCATCACCGTCGATCAATCCGGCAAAGGAAACTTCACCAAAGTACAATCCGCCATTGATGCTGTTCCTTCAAACAACAAGCACTGGGTTTGTATCAAGATCTCCTCCGGAACCTATAGGGAGAAGGTGAAAATTCCTTCTGATAAGCCGTACATAATTCTAAAAGGAGAAAACAAGCGACGAACACAAATCGTTTGGGATGATATTGGCCCTGTTGACAGAAGTGCCACTTTCATTTCTGAAGCTCCTAGTATCACTATCAAATCCATCACTTTTGCTAATTCATACAATAATCCTTTTAACAGATATCGTCAAATTACACAAGCATTGGCAGCTATGATAAGTGGCGATAAAACATTATTCTACAAATGTGGATTTTTTGGGTTTCAAGATACTTTGTGGGCTGTTGAAGGACGCCAGTTTTACAAGAAATGCACTATCCAAGGTGCTATCGATTTCATCTTTGGCCATGCCCAGGCCGTTTTCCAGGGTTGCGCCATATCAGTGCTAGCGGGAAATCTCCAGCCTGGTGGGATAGGGTACATCACAGCACACGGGAGAGACAATCCGGATGAATCAGGCGGATTCATTTTCAACGAATGCATGGTGCATGGTACTGGTTTAACCTATTTGGGAAGGGCGTGGAGACCTTATGCAAGGGTCATTTTCTACAGGTCTAACTTATCAAACATTGTAGTCCCTGAAGGTTGGGATGGTTGGCAAGCTCTTGGTCATGAGGAGCAATTGACATTTGGAGAGTATGGATGCTATGGAGCAGGAGCCAACACTTCGAAGCGTGTGAGTTGGGAAAGGAAGCTTGGCCCTGATATGGTGCAACAGTTTACTAGTTTGAATTTTATTGATTCTGAAGGATGGCTCATTAACCAACCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

342

Amino Acids

38.13

Weight (kDa)

8.98

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 45 - 332 1.1e-67 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 23
AccIII TCCGGA 2 cut(s) 236, 697
AciI CCGC 3 cut(s) 180, 641, 711
AcoI YGGCCR 3 cut(s) 85, 106, 595
AcsI RAATTY 2 cut(s) 261, 982
AcuI CTGAAG 3 cut(s) 390, 852, 1017
AcyI GRCGYC 1 cut(s) 544
AfaI GTAC 5 cut(s) 79, 174, 281, 671, 745
AfiI CCNNNNNNNGG 3 cut(s) 23, 604, 958
AgsI TTSAA 5 cut(s) 201, 518, 539, 724, 982
AhlI ACTAGT 1 cut(s) 974
AjnI CCWGG 3 cut(s) 603, 615, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 930, 962
AluBI AGCT 5 cut(s) 122, 374, 464, 854, 946
AluI AGCT 5 cut(s) 122, 374, 464, 854, 946
Alw26I GTCTC 2 cut(s) 684, 769
Aor13HI TCCGGA 2 cut(s) 236, 697
AoxI GGCC 6 cut(s) 85, 106, 337, 595, 606, 949
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 261, 982
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 2 cut(s) 338, 950
AsuHPI GGTGA 3 cut(s) 127, 157, 268
AsuII TTCGAA 1 cut(s) 920
AsuNHI GCTAGC 1 cut(s) 637
BalI TGGCCA 2 cut(s) 87, 597
BbvI GCAGC 1 cut(s) 473
BccI CCATC 4 cut(s) 140, 404, 836, 996
BceAI ACGGC 3 cut(s) 121, 262, 593
BciT130I CCWGG 3 cut(s) 605, 617, 657
BcoDI GTCTC 2 cut(s) 684, 769
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 5 cut(s) 53, 74, 378, 638, 975
BfmI CTRYAG 3 cut(s) 94, 244, 799
BglII AGATCT 2 cut(s) 90, 228
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 3 cut(s) 605, 617, 657
BmgT120I GGNCC 2 cut(s) 338, 950
BmiI GGNNCC 2 cut(s) 241, 910
BmrFI CCNGG 3 cut(s) 605, 617, 657
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 2 cut(s) 178, 884
BmtI GCTAGC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 635
Bpu14I TTCGAA 1 cut(s) 920
Bsa29I ATCGAT 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 544
BsaI GGTCTC 1 cut(s) 769
BsaJI CCNNGG 4 cut(s) 17, 571, 603, 616
BsaWI WCCGGW 2 cut(s) 236, 697
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 604, 958
Bse1I ACTGG 3 cut(s) 218, 547, 751
BseAI TCCGGA 2 cut(s) 236, 697
BseBI CCWGG 3 cut(s) 605, 617, 657
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 4 cut(s) 17, 571, 603, 616
BseGI GGATG 5 cut(s) 334, 706, 847, 899, 1007
BseLI CCNNNNNNNGG 3 cut(s) 23, 604, 958
BseNI ACTGG 3 cut(s) 218, 547, 751
BseRI GAGGAG 2 cut(s) 223, 881
BseXI GCAGC 1 cut(s) 473
BshFI GGCC 6 cut(s) 87, 108, 339, 597, 608, 951
BshVI ATCGAT 1 cut(s) 582
BsiSI CCGG 3 cut(s) 150, 237, 698
BslFI GGGAC 1 cut(s) 812
BslI CCNNNNNNNGG 3 cut(s) 23, 604, 958
BsmAI GTCTC 2 cut(s) 684, 769
BsmFI GGGAC 1 cut(s) 812
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 6 cut(s) 87, 108, 339, 597, 608, 951
Bso31I GGTCTC 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 920
Bsp13I TCCGGA 2 cut(s) 236, 697
Bsp143I GATC 3 cut(s) 90, 142, 228
Bsp19I CCATGG 1 cut(s) 17
BspACI CCGC 3 cut(s) 180, 641, 711
BspANI GGCC 6 cut(s) 87, 108, 339, 597, 608, 951
BspDI ATCGAT 1 cut(s) 582
BspEI TCCGGA 2 cut(s) 236, 697
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 2 cut(s) 241, 910
BspOI GCTAGC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 769
BsrI ACTGG 3 cut(s) 218, 547, 751
BssECI CCNNGG 4 cut(s) 17, 571, 603, 616
BssMI GATC 3 cut(s) 90, 142, 228
BssNI GRCGYC 1 cut(s) 544
BssT1I CCWWGG 2 cut(s) 17, 571
Bst2UI CCWGG 3 cut(s) 605, 617, 657
Bst4CI ACNGT 2 cut(s) 139, 969
BstACI GRCGYC 1 cut(s) 544
BstBI TTCGAA 1 cut(s) 920
BstC8I GCNNGC 2 cut(s) 639, 852
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 5 cut(s) 334, 706, 847, 899, 1007
BstHHI GCGC 1 cut(s) 626
BstKTI GATC 3 cut(s) 93, 145, 231
BstMAI GTCTC 2 cut(s) 684, 769
BstMBI GATC 3 cut(s) 90, 142, 228
BstMWI GCNNNNNNNGC 2 cut(s) 188, 461
BstNI CCWGG 3 cut(s) 605, 617, 657
BstSCI CCNGG 3 cut(s) 603, 615, 655
BstSFI CTRYAG 3 cut(s) 94, 244, 799
BstV1I GCAGC 1 cut(s) 473
BstX2I RGATCY 2 cut(s) 90, 228
BstXI CCANNNNNNTGG 1 cut(s) 659
BstYI RGATCY 2 cut(s) 90, 228
Bsu15I ATCGAT 1 cut(s) 582
BsuRI GGCC 6 cut(s) 87, 108, 339, 597, 608, 951
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 5 cut(s) 334, 706, 847, 899, 1007
BtsIMutI CAGTG 2 cut(s) 211, 639
Cac8I GCNNGC 2 cut(s) 639, 852
CciI TCATGA 1 cut(s) 862
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 338, 950
ClaI ATCGAT 1 cut(s) 582
CseI GACGC 1 cut(s) 552
Csp6I GTAC 5 cut(s) 78, 173, 280, 670, 744
CviAII CATG 6 cut(s) 18, 83, 599, 733, 740, 863
CviQI GTAC 5 cut(s) 78, 173, 280, 670, 744
DpnI GATC 3 cut(s) 92, 144, 230
DpnII GATC 3 cut(s) 90, 142, 228
EaeI YGGCCR 3 cut(s) 85, 106, 595
EciI GGCGGA 2 cut(s) 169, 726
Eco130I CCWWGG 2 cut(s) 17, 571
Eco31I GGTCTC 1 cut(s) 769
Eco32I GATATC 1 cut(s) 437
Eco57I CTGAAG 3 cut(s) 390, 852, 1017
EcoRII CCWGG 3 cut(s) 603, 615, 655
EcoRV GATATC 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 17, 571
EcoT22I ATGCAT 1 cut(s) 734
ErhI CCWWGG 2 cut(s) 17, 571
FaeI CATG 6 cut(s) 21, 86, 602, 736, 743, 866
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 1 cut(s) 812
FatI CATG 6 cut(s) 17, 82, 598, 732, 739, 862
FauI CCCGC 1 cut(s) 634
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 5 cut(s) 341, 713, 854, 906, 1014
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 5 cut(s) 53, 74, 378, 638, 975
GlaI GCGC 1 cut(s) 625
GluI GCNGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 6 cut(s) 87, 108, 339, 597, 608, 951
HapII CCGG 3 cut(s) 150, 237, 698
HgaI GACGC 1 cut(s) 552
HhaI GCGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 544
Hin1II CATG 6 cut(s) 21, 86, 602, 736, 743, 866
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 3 cut(s) 704, 714, 992
HpaII CCGG 3 cut(s) 150, 237, 698
HphI GGTGA 3 cut(s) 127, 157, 268
Hpy166II GTNNAC 2 cut(s) 346, 972
Hpy188I TCNGA 3 cut(s) 271, 370, 997
Hpy188III TCNNGA 5 cut(s) 226, 237, 518, 698, 863
Hpy8I GTNNAC 2 cut(s) 346, 972
Hpy99I CGWCG 2 cut(s) 143, 312
HpyAV CCTTC 7 cut(s) 207, 247, 276, 533, 759, 827, 992
HpyCH4III ACNGT 2 cut(s) 139, 969
HpyCH4V TGCA 5 cut(s) 564, 732, 739, 785, 964
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 461
Hsp92I GRCGYC 1 cut(s) 544
Hsp92II CATG 6 cut(s) 21, 86, 602, 736, 743, 866
HspAI GCGC 1 cut(s) 624
Kpn2I TCCGGA 2 cut(s) 236, 697
Kzo9I GATC 3 cut(s) 90, 142, 228
LmnI GCTCC 4 cut(s) 379, 868, 902, 908
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 178, 884
MaeI CTAG 5 cut(s) 53, 74, 378, 638, 975
MaeIII GTNAC 1 cut(s) 26
MalI GATC 3 cut(s) 92, 144, 230
MboI GATC 3 cut(s) 90, 142, 228
MfeI CAATTG 1 cut(s) 872
MflI RGATCY 2 cut(s) 90, 228
MlsI TGGCCA 2 cut(s) 87, 597
MluCI AATT 7 cut(s) 116, 261, 285, 409, 444, 872, 982
MluNI TGGCCA 2 cut(s) 87, 597
MnlI CCTC 3 cut(s) 24, 244, 859
Mox20I TGGCCA 2 cut(s) 87, 597
Mph1103I ATGCAT 1 cut(s) 734
MroI TCCGGA 2 cut(s) 236, 697
MscI TGGCCA 2 cut(s) 87, 597
MseI TTAA 4 cut(s) 429, 752, 1011, 1024
MslI CAYNNNNRTG 1 cut(s) 632
Msp20I TGGCCA 2 cut(s) 87, 597
MspI CCGG 3 cut(s) 150, 237, 698
MspR9I CCNGG 3 cut(s) 605, 617, 657
MunI CAATTG 1 cut(s) 872
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 3 cut(s) 605, 617, 657
MwoI GCNNNNNNNGC 2 cut(s) 188, 461
NcoI CCATGG 1 cut(s) 17
NdeII GATC 3 cut(s) 90, 142, 228
NheI GCTAGC 1 cut(s) 637
NlaIII CATG 6 cut(s) 21, 86, 602, 736, 743, 866
NlaIV GGNNCC 2 cut(s) 241, 910
NsiI ATGCAT 1 cut(s) 734
NspV TTCGAA 1 cut(s) 920
PagI TCATGA 1 cut(s) 862
PctI GAATGC 1 cut(s) 734
PfeI GAWTC 3 cut(s) 704, 714, 992
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 1 cut(s) 463
Psp6I CCWGG 3 cut(s) 603, 615, 655
PspGI CCWGG 3 cut(s) 603, 615, 655
PspN4I GGNNCC 2 cut(s) 241, 910
PspPI GGNCC 2 cut(s) 338, 950
PsuI RGATCY 2 cut(s) 90, 228
RsaI GTAC 5 cut(s) 79, 174, 281, 671, 745
RsaNI GTAC 5 cut(s) 78, 173, 280, 670, 744
RseI CAYNNNNRTG 1 cut(s) 632
SaqAI TTAA 4 cut(s) 429, 752, 1011, 1024
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 3 cut(s) 90, 142, 228
Sau96I GGNCC 2 cut(s) 338, 950
ScrFI CCNGG 3 cut(s) 605, 617, 657
SfaNI GCATC 2 cut(s) 178, 884
SfcI CTRYAG 3 cut(s) 94, 244, 799
SfuI TTCGAA 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 632
SpeI ACTAGT 1 cut(s) 974
Sse9I AATT 7 cut(s) 116, 261, 285, 409, 444, 872, 982
SsiI CCGC 3 cut(s) 180, 641, 711
SspI AATATT 1 cut(s) 9
SspMI CTAG 5 cut(s) 53, 74, 378, 638, 975
StyD4I CCNGG 3 cut(s) 603, 615, 655
StyI CCWWGG 2 cut(s) 17, 571
TaaI ACNGT 2 cut(s) 139, 969
TaqI TCGA 3 cut(s) 141, 582, 920
TasI AATT 7 cut(s) 116, 261, 285, 409, 444, 872, 982
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 3 cut(s) 704, 714, 992
Tru1I TTAA 4 cut(s) 429, 752, 1011, 1024
Tru9I TTAA 4 cut(s) 429, 752, 1011, 1024
TscAI CASTG 2 cut(s) 218, 639
TseI GCWGC 1 cut(s) 461
TspDTI ATGAA 6 cut(s) 24, 352, 402, 577, 706, 717
TspRI CASTG 2 cut(s) 218, 639
Van91I CCANNNNNTGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 261, 982
XspI CTAG 5 cut(s) 53, 74, 378, 638, 975
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.