Rh4CG205800

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015806
Physical Location & Seq
Forward (+)
43332670 .. 43334954
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG205800.1

Sequence Viewer

Length: 1020 bp
ATGTCATTTCTTCTTCGATGGTTAGCAATTGTTGCCATCTTGCTAGGGATTGGATTATTAGGGCAACTAGGTGTAGATGGCCAGGTCTATAAGTTGTACGGCCAAAAGAAATTGGCTACTTACACCATCACCGTAGATCAATCCGGCCAAGGAAACTTCACCAAAATACAATCTGCCATTGATGCTGTTCCTTCAAACAACAAGCATTGGGTTTGCATCAAGATCTCCGCCGGCACCTATAGGGAGAAGGTGAAAATCCCTTTTGATAAGCCGTACATACTTCTGAAAGGAGAAAACAAGCGACAAACACAAATTGTTTGGGATGATATTGGCCCTGTTGACCAAAGTGCCACTTTCACTTCTGAAGCCCCTAGTATCACTGTCAAATCTATCACTTTTGCTAATTCATACAACAATCCTTTTAATAGCAATCGTCAAATTACACAAGCATTGGCAGCGATGATAAGTGGTGACAAAACTTCCTTCTATAAATGTGGATTCTTTGGGTTTCAAGACACTTTGTGGGCGGTTGAAGGACGCCACTTTTACAAGCATTGCACTATCCAAGGTGCTATTGATTTTATCTTTGGCCATGCCCAGGCCATTTTCCAGGGATGCTCCATATCAGTGCTTGCGGGAAATCTGCAGCCCGGTGGGATAGGATACATCACAGCACAGGGGAGAGAACAACCAAATGAACCTGGTGCTTTTATTTTCAAGAATTGCAGGGTGCATGGTACTGGTTTAACCTATTTGGGAAGGGCATGGAGACTGTACTCGAGGGTCATTTTCTACGATTCCAACTTATCAGACATTGTAGTCCCTCAGGGTTGGGATGGTTGGCAGGCTCTTGGCCATGAGGACCTATTGACATTTGCCGAGTATGGGTGCTATGGTCCAGGAGCCGACACTTCGAAGCGTGTGAGTTGGGAAAAGAAGCTTAGCCCTAATATCGTGCAACAGTTTACTAGCTTGAATTTCATTGATTCTGATGGATGGCTCATTGACCAGCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

339

Amino Acids

37.69

Weight (kDa)

8.41

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 43 - 330 7.9e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 3 cut(s) 228, 527, 635
AcoI YGGCCR 5 cut(s) 79, 100, 145, 589, 853
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 538
AdeI CACNNNGTG 1 cut(s) 522
AfaI GTAC 4 cut(s) 98, 275, 739, 776
AfiI CCNNNNNNNGG 2 cut(s) 598, 885
AgsI TTSAA 5 cut(s) 195, 512, 533, 718, 976
AjnI CCWGG 5 cut(s) 81, 597, 609, 700, 898
AluBI AGCT 2 cut(s) 940, 972
AluI AGCT 2 cut(s) 940, 972
Alw26I GTCTC 1 cut(s) 763
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 7 cut(s) 79, 100, 145, 331, 589, 600, 853
ApeKI GCWGC 2 cut(s) 455, 646
ApoI RAATTY 1 cut(s) 976
AspS9I GGNCC 3 cut(s) 332, 862, 896
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 4 cut(s) 121, 151, 262, 482
AsuII TTCGAA 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 2 cut(s) 862, 896
AxyI CCTNAGG 1 cut(s) 825
BalI TGGCCA 3 cut(s) 81, 591, 855
BanI GGYRCC 1 cut(s) 233
BbvI GCAGC 2 cut(s) 467, 658
BccI CCATC 7 cut(s) 12, 44, 71, 134, 830, 986, 990
BceAI ACGGC 2 cut(s) 115, 256
BciT130I CCWGG 5 cut(s) 83, 599, 611, 702, 900
BciVI GTATCC 1 cut(s) 656
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 763
BfaI CTAG 4 cut(s) 44, 68, 372, 969
BfmI CTRYAG 2 cut(s) 238, 644
BfuI GTATCC 1 cut(s) 656
BglII AGATCT 1 cut(s) 222
BisI GCNGC 2 cut(s) 456, 647
BlpI GCTNAGC 1 cut(s) 941
BlsI GCNGC 2 cut(s) 457, 648
Bme1390I CCNGG 6 cut(s) 83, 599, 611, 651, 702, 900
Bme18I GGWCC 2 cut(s) 862, 896
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 332, 862, 896
BmiI GGNNCC 2 cut(s) 235, 904
BmrFI CCNGG 6 cut(s) 83, 599, 611, 651, 702, 900
BmsI GCATC 3 cut(s) 172, 225, 605
Bpu1102I GCTNAGC 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 914
BpuMI CCSGG 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 148, 565, 597, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 885
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 745
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 553
BseBI CCWGG 5 cut(s) 83, 599, 611, 702, 900
BseDI CCNNGG 4 cut(s) 148, 565, 597, 610
BseGI GGATG 4 cut(s) 328, 620, 841, 1001
BseLI CCNNNNNNNGG 2 cut(s) 598, 885
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 1 cut(s) 745
BseXI GCAGC 2 cut(s) 467, 658
BshFI GGCC 7 cut(s) 81, 102, 147, 333, 591, 602, 855
BshNI GGYRCC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 3 cut(s) 144, 231, 651
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 2 cut(s) 598, 885
BsmAI GTCTC 1 cut(s) 763
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 7 cut(s) 81, 102, 147, 333, 591, 602, 855
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 1 cut(s) 914
Bsp143I GATC 2 cut(s) 136, 222
Bsp1720I GCTNAGC 1 cut(s) 941
BspACI CCGC 3 cut(s) 228, 527, 635
BspANI GGCC 7 cut(s) 81, 102, 147, 333, 591, 602, 855
BspCNI CTCAG 1 cut(s) 838
BspLI GGNNCC 2 cut(s) 235, 904
BspMAI CTGCAG 1 cut(s) 648
BspT104I TTCGAA 1 cut(s) 914
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 745
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 4 cut(s) 148, 565, 597, 610
BssMI GATC 2 cut(s) 136, 222
BssNI GRCGYC 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 148, 565
Bst2UI CCWGG 5 cut(s) 83, 599, 611, 702, 900
Bst4CI ACNGT 4 cut(s) 133, 382, 774, 963
BstACI GRCGYC 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 914
BstC8I GCNNGC 3 cut(s) 232, 633, 846
BstDEI CTNAG 2 cut(s) 825, 941
BstF5I GGATG 4 cut(s) 328, 620, 841, 1001
BstKTI GATC 2 cut(s) 139, 225
BstMAI GTCTC 1 cut(s) 763
BstMBI GATC 2 cut(s) 136, 222
BstMWI GCNNNNNNNGC 3 cut(s) 32, 182, 455
BstNI CCWGG 5 cut(s) 83, 599, 611, 702, 900
BstSCI CCNGG 6 cut(s) 81, 597, 609, 649, 700, 898
BstSFI CTRYAG 2 cut(s) 238, 644
BstV1I GCAGC 2 cut(s) 467, 658
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 825
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 7 cut(s) 81, 102, 147, 333, 591, 602, 855
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 4 cut(s) 328, 620, 841, 1001
BtsIMutI CAGTG 2 cut(s) 378, 633
Cac8I GCNNGC 3 cut(s) 232, 633, 846
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 332, 862, 896
CseI GACGC 1 cut(s) 546
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 4 cut(s) 97, 274, 738, 775
CviAII CATG 4 cut(s) 593, 734, 765, 857
CviQI GTAC 4 cut(s) 97, 274, 738, 775
DdeI CTNAG 2 cut(s) 825, 941
DpnI GATC 2 cut(s) 138, 224
DpnII GATC 2 cut(s) 136, 222
DraIII CACNNNGTG 1 cut(s) 522
DrdI GACNNNNNNGTC 1 cut(s) 818
DseDI GACNNNNNNGTC 1 cut(s) 818
EaeI YGGCCR 5 cut(s) 79, 100, 145, 589, 853
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 148, 565
Eco47I GGWCC 2 cut(s) 862, 896
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 862
EcoRII CCWGG 5 cut(s) 81, 597, 609, 700, 898
EcoT14I CCWWGG 2 cut(s) 148, 565
ErhI CCWWGG 2 cut(s) 148, 565
FaeI CATG 4 cut(s) 596, 737, 768, 860
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 1 cut(s) 806
FatI CATG 4 cut(s) 592, 733, 764, 856
FauI CCCGC 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 456, 647
FokI GGATG 4 cut(s) 335, 627, 848, 1008
Fsp4HI GCNGC 2 cut(s) 456, 647
FspBI CTAG 4 cut(s) 44, 68, 372, 969
GluI GCNGC 2 cut(s) 456, 647
HaeIII GGCC 7 cut(s) 81, 102, 147, 333, 591, 602, 855
HapII CCGG 3 cut(s) 144, 231, 651
HgaI GACGC 1 cut(s) 546
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 4 cut(s) 596, 737, 768, 860
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 3 cut(s) 498, 797, 986
HpaII CCGG 3 cut(s) 144, 231, 651
HphI GGTGA 4 cut(s) 121, 151, 262, 482
Hpy166II GTNNAC 2 cut(s) 340, 966
Hpy188I TCNGA 4 cut(s) 285, 364, 811, 991
Hpy188III TCNNGA 3 cut(s) 220, 512, 718
Hpy8I GTNNAC 2 cut(s) 340, 966
HpyAV CCTTC 5 cut(s) 201, 241, 493, 527, 753
HpyCH4III ACNGT 4 cut(s) 133, 382, 774, 963
HpyCH4V TGCA 6 cut(s) 216, 558, 646, 726, 733, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 182, 455
HpyF3I CTNAG 2 cut(s) 825, 941
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 4 cut(s) 596, 737, 768, 860
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 136, 222
LmnI GCTCC 2 cut(s) 623, 902
Lsp1109I GCAGC 2 cut(s) 467, 658
LweI GCATC 3 cut(s) 172, 225, 605
MabI ACCWGGT 1 cut(s) 700
MaeI CTAG 4 cut(s) 44, 68, 372, 969
MaeIII GTNAC 1 cut(s) 470
MalI GATC 2 cut(s) 138, 224
MboI GATC 2 cut(s) 136, 222
MboII GAAGA 1 cut(s) 5
MfeI CAATTG 1 cut(s) 27
MflI RGATCY 1 cut(s) 222
MlsI TGGCCA 3 cut(s) 81, 591, 855
MluCI AATT 7 cut(s) 27, 110, 312, 403, 438, 721, 976
MluNI TGGCCA 3 cut(s) 81, 591, 855
MmeI TCCRAC 1 cut(s) 825
MnlI CCTC 3 cut(s) 774, 834, 853
Mox20I TGGCCA 3 cut(s) 81, 591, 855
MroNI GCCGGC 1 cut(s) 230
MscI TGGCCA 3 cut(s) 81, 591, 855
MseI TTAA 2 cut(s) 423, 746
MslI CAYNNNNRTG 1 cut(s) 626
Msp20I TGGCCA 3 cut(s) 81, 591, 855
MspI CCGG 3 cut(s) 144, 231, 651
MspR9I CCNGG 6 cut(s) 83, 599, 611, 651, 702, 900
MunI CAATTG 1 cut(s) 27
MvaI CCWGG 5 cut(s) 83, 599, 611, 702, 900
MwoI GCNNNNNNNGC 3 cut(s) 32, 182, 455
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 651
NdeII GATC 2 cut(s) 136, 222
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 596, 737, 768, 860
NlaIV GGNNCC 2 cut(s) 235, 904
NmeAIII GCCGAG 1 cut(s) 904
NmuCI GTSAC 1 cut(s) 470
NspV TTCGAA 1 cut(s) 914
PaeR7I CTCGAG 1 cut(s) 778
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 3 cut(s) 498, 797, 986
PfoI TCCNGGA 1 cut(s) 898
PkrI GCNGC 2 cut(s) 457, 648
PpuMI RGGWCCY 1 cut(s) 862
Psp5II RGGWCCY 1 cut(s) 862
Psp6I CCWGG 5 cut(s) 81, 597, 609, 700, 898
PspGI CCWGG 5 cut(s) 81, 597, 609, 700, 898
PspN4I GGNNCC 2 cut(s) 235, 904
PspPI GGNCC 3 cut(s) 332, 862, 896
PspPPI RGGWCCY 1 cut(s) 862
PspXI VCTCGAGB 1 cut(s) 778
PstI CTGCAG 1 cut(s) 648
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 4 cut(s) 98, 275, 739, 776
RsaNI GTAC 4 cut(s) 97, 274, 738, 775
RseI CAYNNNNRTG 1 cut(s) 626
SaqAI TTAA 2 cut(s) 423, 746
SatI GCNGC 2 cut(s) 456, 647
Sau3AI GATC 2 cut(s) 136, 222
Sau96I GGNCC 3 cut(s) 332, 862, 896
ScrFI CCNGG 6 cut(s) 83, 599, 611, 651, 702, 900
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 3 cut(s) 172, 225, 605
SfcI CTRYAG 2 cut(s) 238, 644
Sfr274I CTCGAG 1 cut(s) 778
SfuI TTCGAA 1 cut(s) 914
SinI GGWCC 2 cut(s) 862, 896
SlaI CTCGAG 1 cut(s) 778
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 778
SmoI CTYRAG 1 cut(s) 778
Sse9I AATT 7 cut(s) 27, 110, 312, 403, 438, 721, 976
SsiI CCGC 3 cut(s) 228, 527, 635
SspMI CTAG 4 cut(s) 44, 68, 372, 969
StyD4I CCNGG 6 cut(s) 81, 597, 609, 649, 700, 898
StyI CCWWGG 2 cut(s) 148, 565
TaaI ACNGT 4 cut(s) 133, 382, 774, 963
TaqI TCGA 3 cut(s) 16, 779, 914
TasI AATT 7 cut(s) 27, 110, 312, 403, 438, 721, 976
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 3 cut(s) 498, 797, 986
Tru1I TTAA 2 cut(s) 423, 746
Tru9I TTAA 2 cut(s) 423, 746
TscAI CASTG 2 cut(s) 385, 633
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 455, 646
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 396, 711, 970
TspRI CASTG 2 cut(s) 385, 633
VpaK11BI GGWCC 2 cut(s) 862, 896
XapI RAATTY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 778
XspI CTAG 4 cut(s) 44, 68, 372, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.